Journal of Agricultural and Food Chemistry最新文献_第8页

Multisite Mutagenesis of 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) Enhances Rice Resistance to HPPD Inhibitors and Its Carotenoid Contents 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) 的多位点突变增强了水稻对 HPPD 抑制剂的抗性及其类胡萝卜素含量

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-25 DOI: 10.1021/acs.jafc.4c05600

Yuwen Yang, Zhenzhen Zhou, Tingli Liu, Qing Tan, Le Chen, Jinyan Wang, Zeyu Qiu, Xitie Ling, Tianzi Chen, Xia Yang, Qing Liu, Dongshu Guo, Baolong Zhang

While frequently used herbicides display limited efficacy against herbicide-resistant weeds, it becomes imperative to explore novel herbicides that ensure both effective weed management and environmental safety. Though 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitory herbicides like mesotrione are prevalent in maize weed management, their integration into rice production is hindered due to the inherent sensitivity of rice HPPD (OsHPPD). In this study, a mutant allele of OsHPPD featuring six amino acid substitutions, termed OsHPPD-6M, maintains enzymatic activity in 200 μm mesotrione while the wild type can only withstand 1 μm. Enzymatic assays in vitro indicated that the HPPD activity of OsHPPD-6M surpassed that of the WT by 2-fold through enhanced substrate-binding. Its overexpression in transgenic rice conferred greater tolerance to mesotrione, topramezone, and isoxaflutole by 36.7-, 41.6-, and 37.1-fold relative to that in the WT rice. Interestingly, these 6M-OE plants demonstrated substantially elevated contents of carotenoids compared to WT plants without a significant impact on agronomic traits.

常用的除草剂对抗药性杂草的防效有限，因此必须探索新型除草剂，以确保有效的杂草管理和环境安全。尽管 4-羟基苯基丙酮酸二氧酶（HPPD）抑制性除草剂（如美索三酮）在玉米杂草管理中非常普遍，但由于水稻 HPPD（OsHPPD）固有的敏感性，它们在水稻生产中的应用受到了阻碍。在这项研究中，OsHPPD 的一个突变等位基因（称为 OsHPPD-6M）具有六个氨基酸取代，能在 200 μm 的介草酮中保持酶活性，而野生型只能承受 1 μm 的介草酮。体外酶促实验表明，OsHPPD-6M 的 HPPD 活性比 WT 高出 2 倍，原因是底物结合力增强。在转基因水稻中，OsHPPD-6M 的过表达使其对介草酮、托吡酮和异噁唑草酮的耐受性比 WT 水稻分别提高了 36.7 倍、41.6 倍和 37.1 倍。有趣的是，与 WT 植物相比，这些 6M-OE 植物的类胡萝卜素含量大幅提高，但对农艺性状没有显著影响。

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引用次数: 0

Fungicidal Activity of Novel 6-Isothiazol-5-ylpyrimidin-4-amine-Containing Compounds Targeting Complex I Reduced Nicotinamide Adenine Dinucleotide Oxidoreductase. 以还原型烟酰胺腺嘌呤二核苷酸氧化还原酶 I 复合物为靶点的新型 6-异噻唑-5-基嘧啶-4-胺化合物的杀菌活性

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-25 DOI: 10.1021/acs.jafc.4c07259

Kun Li,You Lv,Rongzhang Wu,Zhenwu Yu,Yulin Liang,Zecong Yu,Ruobing Liang,Liangfu Tang,Hongyu Chen,Zhijin Fan

To discover novel inhibitors of the complex I reduced nicotinamide adenine dinucleotide (NADH) oxidoreductase as fungicides, a series of 6-isothiazol-5-ylpyrimidin-4-amine-containing compounds were designed using a computer-aided pesticide design method and splicing of substructures from diflumetorim and isotianil. In vitro fungicidal bioassays indicated that compounds T17-T24 showed high inhibitory activity against Rhizoctonia solani with an effective concentration (EC50) value falling between 2.20 and 23.85 μg/mL, which were more active than or equivalent to the lead diflumetorim with its EC50 of 19.80 μg/mL. In vivo antifungal bioassays demonstrated that, at a concentration of 200 μg/mL, T7 and T21 showed higher inhibition against Pseudoperonospora cubensis than all other compounds, while T23 exhibited the highest inhibition against Sphaerotheca fuliginea. T23 showed an approximately twofold lower inhibition potency against R. solani complex I NADH oxidoreductase than diflumetorim. Molecular docking and transcriptomic analyses indicated that T23 and diflumetorim both might share the same mode of action, targeting NADH oxidoreductase. T23 as a good fungicidal candidate against R. solani is worthy of further investigation.

为了发现可作为杀菌剂的复合物 I 还原型烟酰胺腺嘌呤二核苷酸（NADH）氧化还原酶的新型抑制剂，我们采用计算机辅助农药设计方法，通过二氟甲嘧啶和异噻菌胺的子结构拼接，设计了一系列含 6-异噻唑-5-基嘧啶-4-胺的化合物。体外杀菌生物测定结果表明，化合物 T17-T24 对根瘤菌表现出较高的抑制活性，有效浓度（EC50）值介于 2.20 至 23.85 μg/mL 之间，活性高于或相当于二氟甲嘧啶（其 EC50 值为 19.80 μg/mL）。体内抗真菌生物测定表明，在 200 μg/mL 的浓度下，T7 和 T21 对立方假包囊孢子菌的抑制率高于所有其他化合物，而 T23 对富贵竹孢子菌的抑制率最高。T23 对 R. solani 复合物 I NADH 氧化还原酶的抑制效力比双氟美托林低大约两倍。分子对接和转录组分析表明，T23 和二氟美托林可能具有相同的作用模式，都以 NADH 氧化还原酶为靶标。T23 作为一种很好的候选杀真菌剂，值得进一步研究。

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引用次数: 0

Galangin Alleviates Alcohol-Provoked Liver Injury Associated with Gut Microbiota Disorder and Intestinal Barrier Dysfunction in Mice 高良姜素能缓解酒精诱发的肝损伤以及小鼠肠道微生物群紊乱和肠屏障功能障碍

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-25 DOI: 10.1021/acs.jafc.4c05617

Yanan Zhao, Bin Li, Hongting Deng, Chang Zhang, Yitong Wang, Lei Chen, Hui Teng

Prolonged and excessive intake of alcohol results in the onset of alcoholic liver disease, which is marked by oxidative stress, intestinal barrier dysfunction, and disturbance in the intestinal microbiome. Galangin, a potent flavonoid from Alpinia officinarum Hance, has been recognized for its diverse biological properties; however, its ability for protecting against alcohol-stimulated hepatotoxicity remains unexplored in prior research. In the current study, a Gao-Binge mouse model was established to assess the positive role and mechanisms of galangin upon alcohol-induced liver injury. The administration of galangin relieved liver pathological damage, oxidative stress, and NLRP3-mediated inflammation induced by alcohol. In addition, galangin significantly reversed abnormal intestinal histopathological manifestations and damaged the intestinal barrier function. Furthermore, microbiota composition revealed that galangin improved intestinal imbalance by improving the gut microbiota dysbiosis and short-chain fatty acid level. Collectively, this study explored the interactions between phytochemical factors and virulence factors and discovered that galangin powerfully improved alcohol-induced liver disease by repressing the inflammatory cascade via the gut microbiota-mediated gut–liver axis. These results suggested that alcohol-targeted natural products could have potential applications in promoting food safety and human health and offer valuable insights into the possible use of these substances in these important areas.

长期过量摄入酒精会导致酒精性肝病的发生，其特征是氧化应激、肠道屏障功能障碍和肠道微生物群紊乱。高良姜素是一种从高良姜（Alpinia officinarum Hance）中提取的强效黄酮类化合物，它的多种生物特性已得到公认；然而，在以往的研究中，高良姜素保护肝脏免受酒精刺激的能力仍未得到探索。在本研究中，我们建立了一个 "Gao-Binge "小鼠模型，以评估高良姜素对酒精诱导的肝损伤的积极作用和机制。高良姜素缓解了酒精诱导的肝脏病理损伤、氧化应激和NLRP3介导的炎症反应。此外，高良姜素还能明显逆转异常的肠道组织病理学表现和受损的肠道屏障功能。此外，微生物群组成显示，高良姜素通过改善肠道微生物群失调和短链脂肪酸水平，改善了肠道失衡。总之，本研究探索了植物化学因子与致病因子之间的相互作用，发现高良姜素通过肠道微生物群介导的肠道-肝脏轴抑制炎症级联，从而有力地改善了酒精诱导的肝病。这些结果表明，以酒精为靶标的天然产品在促进食品安全和人类健康方面具有潜在的应用价值，并为这些物质在这些重要领域的可能应用提供了宝贵的见解。

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引用次数: 0

Design, Synthesis, and Antifungal Evaluation of Novel Pyrazole-5-sulfonamide Derivatives for Plant Protection 用于植物保护的新型吡唑-5-磺酰胺衍生物的设计、合成和抗真菌评估

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-25 DOI: 10.1021/acs.jafc.4c05050

Xiang Cheng, Yaping Song, Jiexiu Gong, Fanglei Wang, Dandan Wang, Xihao Chang, Xianhai Lv

To develop further novel environmentally friendly antifungal agents with high efficacy, a series of pyrazole-5-sulfonamide derivatives were designed and synthesized by using the active molecules synthesized in previous works as lead compounds. Their antifungal activities were evaluated in vitro against ten highly destructive plant pathogenic fungi. The bioassay results indicated that more than half of the target compounds displayed potent antifungal activities (inhibition rate ≥85%) against Valsa mali and Sclerotinia sclerotiorum at 20 mg/L. Among them, compound C22 exhibited significant broad-spectrum antifungal activities against V. mali, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, and Trichoderma viride, with EC₅₀ values of 0.45, 0.49, 3.06, 0.57, and 1.43 mg/L, respectively. Moreover, compounds C21 and C22 exhibited remarkable protective effects on apple Valsa canker similar to tebuconazole (89.5%) at 50 mg/L. Preliminary antifungal mechanism investigations demonstrated that compound C22 may have inhibited V. mali mycelial growth by inducing oxidative damage to the mycelium and compromising the integrity of the cell membrane. Meanwhile, compounds C21 and C22 exhibited no obvious toxicity to worker bees (Apis mellifera ligustica). Taken together, these pyrazole-5-sulfonamide derivatives, particularly compound C22, possess huge potential to be developed as novel environmentally friendly fungicides with high efficacy.

为了进一步开发新型高效的环境友好型抗真菌剂，研究人员以之前合成的活性分子为先导化合物，设计并合成了一系列吡唑-5-磺酰胺衍生物。体外评估了这些衍生物对十种高破坏性植物病原真菌的抗真菌活性。生物测定结果表明，半数以上的目标化合物在 20 毫克/升的浓度下对马来疫霉和硬皮病菌具有很强的抗真菌活性（抑制率≥85%）。其中，化合物 C22 对 V.mali、S. sclerotiorum、Rhizoctonia solani、Botrytis cinerea 和 Trichoderma viride 具有显著的广谱抗真菌活性，EC50 值分别为 0.45、0.49、3.06、0.57 和 1.43 mg/L。此外，化合物 C21 和 C22 在 50 毫克/升浓度下对苹果瓦萨腐烂病的保护作用与戊唑醇（89.5%）相似。初步的抗真菌机理研究表明，化合物 C22 可能是通过诱导菌丝氧化损伤和破坏细胞膜完整性来抑制苹果蠹蛾菌丝生长的。同时，化合物 C21 和 C22 对工蜂（Apis mellifera ligustica）无明显毒性。综上所述，这些吡唑-5-磺酰胺衍生物，尤其是化合物 C22，具有巨大的潜力，可开发为新型高效环保杀菌剂。

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引用次数: 0

Mechanochemistry in Glycation Research. 糖化研究中的机理化学。

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-25 Epub Date: 2024-09-06 DOI: 10.1021/acs.jafc.4c05591

Haoran Xing, Varoujan Yaylayan

Mechanochemistry by milling has recently attracted considerable interest for its ability to drive solvent-free chemical transformations exclusively through mechanical energy and at ambient temperatures. Despite its popularity and expanding applications in different fields of chemistry, its impact on Food Science remains limited. This review aims to demonstrate the specific benefits that mechanochemistry can provide in performing controlled glycation, and in "activating" sugar and amino acid mixtures, thereby allowing for continued generation of colors and aromas even after termination of milling. The generated mechanical energy can be tuned under specific conditions either to form only the corresponding Schiff bases and Amadori compounds or to generate their degradation products, as a function of the frequency of the oscillations in combination with the reactivity of the selected substrates. Similarly, its ability to initiate the Strecker degradation and generate pyrazines and Strecker aldehydes was also demonstrated when proteogenic amino acids were milled with glyoxal.

最近，研磨机械化学因其能够在常温下完全通过机械能驱动无溶剂化学转化而引起了广泛关注。尽管机械化学很受欢迎，而且在不同化学领域的应用也在不断扩大，但它对食品科学的影响仍然有限。本综述旨在展示机械化学在进行受控糖化以及 "活化 "糖和氨基酸混合物方面的具体优势，从而使其在研磨结束后仍能继续产生颜色和香味。所产生的机械能可在特定条件下进行调整，要么只形成相应的席夫碱和阿莫多利化合物，要么产生它们的降解产物，这取决于振荡频率与所选底物的反应性。同样，当蛋白氨基酸与乙二醛一起研磨时，也证明了它启动斯特雷克降解并生成吡嗪和斯特雷克醛的能力。

引用次数: 0

Unlocking Industrial Potential: Phase-Transition Coimmobilization of Multienzyme Systems for High-Efficiency Uridine Diphosphate Galactose Production 释放工业潜力：高效生产二磷酸尿苷半乳糖的多酶系统的相变共固定化

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-24 DOI: 10.1021/acs.jafc.4c07173

Yajing Li, Xinrui Yang, Qi Chen, Yuejun Li, Renjun Gao

Transitioning from batch to continuous industrial production often improves the economic returns and production efficiency. Immobilization is a critical strategy that can facilitate this shift. This study refined the previously established method for synthesizing uridine diphosphate galactose (UDP-Gal) by employing thermophilic enzymes. Three thermophilic enzymes (galactokinase, uridine diphosphate glucose pyrophosphorylase, and inorganic pyrophosphatase) were coimmobilized on the pH-responsive carrier Eudragit S-100, promoting enzyme recovery and reuse while their industrial potential was assessed. The coimmobilization system efficiently catalyzed UDP-Gal production, yielding 13.69 mM in 1.5 h, attaining a UTP conversion rate of 91.2% and a space-time yield (STY) of 5.16 g/L/h. Moreover, the system exhibited exceptional reproducibility, retaining 58.9% of its initial activity after five cycles. This research highlighted promising prospects for coimmobilization in industrial synthesis and proposed a novel methodology for enhancing UDP-Gal production in the industry. In addition, the phase-transition property of Eudragit S-100 paves the way for further exploration with the one-pot synthesis of poorly soluble galactosides.

从批量工业生产过渡到连续工业生产往往能提高经济回报和生产效率。固定化是促进这一转变的关键策略。本研究通过使用嗜热酶，改进了之前建立的合成二磷酸尿苷半乳糖（UDP-Gal）的方法。三种嗜热酶（半乳糖激酶、尿苷二磷酸葡萄糖焦磷酸化酶和无机焦磷酸酶）被共固定在 pH 响应载体 Eudragit S-100 上，促进了酶的回收和再利用，同时对其工业潜力进行了评估。该共固定化系统高效催化了 UDP-Gal 的产生，1.5 小时内产生 13.69 mM，UTP 转化率达 91.2%，时空产率（STY）达 5.16 g/L/h。此外，该系统还表现出卓越的可重复性，在五个周期后仍能保持 58.9% 的初始活性。这项研究凸显了共固定化技术在工业合成中的广阔前景，并提出了一种提高工业中 UDP-Gal 产量的新方法。此外，Eudragit S-100 的相变特性为进一步探索一锅合成难溶性半乳糖苷铺平了道路。

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引用次数: 0

Lactoferrin-Based Heteroprotein Systems, From Their Formation Mechanism, Properties, To Applications 基于乳铁蛋白的异种蛋白系统：从形成机制、特性到应用

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-24 DOI: 10.1021/acs.jafc.4c05298

Runxuan Chen, Jiaqi Ding, Yichen Li, Yuyu Zhang, Rui Yang

Lactoferrin (LF) is an important iron-binding glycoprotein found in milk and mucosal secretions. The alkaline lactoferrin can interact with some acidic proteins to form heteroprotein systems with multifunctional properties and a wide range of applications. Lactoferrin can interact with animal and plant proteins mainly through the electrostatic forces, dipolar attraction, and hydrophobic interactions. In this review, the types of heteroprotein complexes formed by the complex coacervation of lactoferrin with other proteins are introduced, including the preparation, structure, and applications. The factors affecting the formation of heteroprotein complexes are described, such as pH, ionic strength, mixing ratio, total protein concentration, and temperature. The issues and challenges in the formation of heteroprotein complexes are also discussed.

乳铁蛋白（LF）是存在于牛奶和粘膜分泌物中的一种重要的铁结合糖蛋白。碱性乳铁蛋白可与一些酸性蛋白质相互作用，形成具有多功能特性和广泛应用的异种蛋白系统。乳铁蛋白主要通过静电力、偶极子吸引力和疏水作用与动植物蛋白质相互作用。本综述介绍了乳铁蛋白与其他蛋白质复合共轭形成的异种蛋白复合物的类型，包括制备、结构和应用。介绍了影响异种蛋白复合物形成的因素，如 pH 值、离子强度、混合比、总蛋白浓度和温度。还讨论了异种蛋白复合物形成过程中的问题和挑战。

引用次数: 0

Sustainable Natural Resources for Aphid Management: β-Ionone and Its Derivatives as Promising Ecofriendly Botanical-Based Products 用于治理蚜虫的可持续自然资源：β-壬酮及其衍生物是前景看好的生态友好型植物产品

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-24 DOI: 10.1021/acs.jafc.4c04053

Shi-Xiang Pan, Cheng Qu, Yan Liu, Zhuo Shi, Xing-Xing Lu, Zhao-Kai Yang, Xiao-Bo Huang, Wan Li, Xue-Sheng Li, Zheng-Xin Zhou, Chen Chen, Chen Luo, Yao-Guo Qin, Xin-Ling Yang

β-Ionone, sustainably derived from Petunia hybrida as a natural bioresource, was identified as a lead compound for integrated aphid management. A series of β-ionone derivatives containing ester groups were designed and synthesized for the purpose of discovering renewable botanical-based products. The odorant-binding protein (OBP) binding test indicated that β-ionone and its derivatives displayed binding affinities with Acyrthosiphon pisum OBP9 (ApisOBP9) and Harmonia axyridis OBP15 (HaxyOBP15). Bioactivity assays revealed that most β-ionone derivatives exhibited a higher repellent activity than that of β-ionone. β-Ionone and derivatives 4g and 4l displayed attractiveness to H. axyridis. Specifically, 4g was a highly promising derivative, possessing good repellent activity against A. pisum and attractiveness to H. axyridis. Molecular dynamics simulations revealed that integrating the hydrophobic ester group into the β-ionone framework strengthened the van der Waals interactions of 4g with ApisOBP9/HaxyOBP15, improving the binding affinity with OBPs and producing higher push–pull activity than β-ionone; 4g also had low toxicity toward nontarget organisms. Thus, 4g is a potential ecofriendly, botanical-based option for aphid management.

从矮牵牛杂交种中可持续提取的β-葱酮是一种天然生物资源，被确定为蚜虫综合治理的先导化合物。为了发现基于植物的可再生产品，我们设计并合成了一系列含有酯基的 β-ionone 衍生物。气味结合蛋白（OBP）结合试验表明，β-ionone 及其衍生物与 Acyrthosiphon pisum OBP9（ApisOBP9）和 Harmonia axyridis OBP15（HaxyOBP15）具有结合亲和力。生物活性测定显示，大多数 β-ionone 衍生物的驱虫活性高于 β-ionone 衍生物。β-ionone 及其衍生物 4g 和 4l 对 H. axyridis 具有吸引力。具体来说，4g 是一种极具潜力的衍生物，对 A. pisum 具有良好的驱避活性，对 H. axyridis 具有吸引力。分子动力学模拟显示，将疏水酯基团整合到 β-ionone 框架中可加强 4g 与 ApisOBP9/HaxyOBP15 的范德华相互作用，提高与 OBPs 的结合亲和力，并产生比 β-ionone 更高的推拉活性；4g 对非目标生物的毒性也很低。因此，4g 是一种潜在的生态友好型植物性蚜虫防治方法。

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引用次数: 0

Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags 利用 SUMO 融合标签增强具有多功能特性的多拷贝粘蛋白型序列的新型胶原蛋白类似物

IF 6.1 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-24 DOI: 10.1021/acs.jafc.4c07179

Zilong Zhao, Weigang Yuwen, Zhiguang Duan, Chenhui Zhu, Daidi Fan

Multifunctional enhanced collagen materials in green biomanufacturing are highly desired yet challenging due to the poor comprehensive performance caused by the adoption of targeting monofunctional peptides. Herein, novel collagen analog design strategy using multicopy tandem of mucin-type sequence (GAPGAPGSQGAPGLQ) derived from human COL1α1 to construct basic building blocks is reported, in which SUMO tag is added to the N-terminal of the protein as a stabilizing core. In particular, novel collagen analogs (named S1506, S1511, S1523, and S1552) with multicopy mucin-type sequences (repeated 6, 11, 23, and 52 times), which were constructed in Escherichia coli, have distinct orientation preferences of functional enhancement (including cell proliferation, differentiation, migration, antioxidant activity, and anti-inflammatory property) compared to COL1α1 in HaCaT and THP-1 cell experiments due to variant three-dimensional structures (the different-length mucin-type polypeptide chains wind around central SUMO tag). Our findings suggest that the innovative protein design and synthesis approaches employed in the construction of these novel S15 proteins have the potential to advance the development of new types of recombinant collagen analogs.

多功能增强型胶原蛋白材料在绿色生物制造中备受期待，但由于采用靶向性单功能肽导致综合性能较差，因此具有挑战性。本文报道了新型胶原蛋白类似物的设计策略，即利用源自人类 COL1α1 的多拷贝串联粘蛋白型序列（GAPGAPGSQGAPGLQ）构建基本构件，并在蛋白的 N 端添加 SUMO 标签作为稳定核心。特别是在大肠杆菌中构建的具有多拷贝粘蛋白型序列（重复 6、11、23 和 52 次）的新型胶原类似物（命名为 S1506、S1511、S1523 和 S1552），具有不同的功能增强取向偏好（包括细胞增殖、分化、迁移、抗氧化等）、在 HaCaT 和 THP-1 细胞实验中，与 COL1α1 相比，它们的功能增强（包括细胞增殖、分化、迁移、抗氧化活性和抗炎特性）具有不同的取向偏好，这是因为它们的三维结构不同（不同长度的粘蛋白型多肽链缠绕在中心 SUMO 标签周围）。我们的研究结果表明，在构建这些新型 S15 蛋白时所采用的创新蛋白质设计和合成方法有望推动新型重组胶原类似物的开发。

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引用次数: 0

Identification of a Novel Ibuprofen Biotransformation Pathway in Streptomyces sp. D218 and Detoxification as Indicated by the Green Algae Scenedesmus obliquus. 鉴定链霉菌 D218 中布洛芬的新型生物转化途径以及绿藻 Scenedesmus obliquus 的解毒作用。

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry

Pub Date : 2024-09-24 DOI: 10.1021/acs.jafc.4c05967

Junwei Huang, Zilin Zhu, Ruomu Chen, Dandan Pan, Qing X Li, Xiangwei Wu

Ibuprofen, a widely used nonsteroidal anti-inflammatory drug, contaminates agricultural products and potentially threatens human health due to its frequent detection and poor biodegradability. Microbial metabolism dominates the elimination of residual ibuprofen in the environment. In mineral salt medium at pH 6 with 5 mM glucose, Streptomyces sp. D218 transformed ibuprofen concentrations ranging from 0.05 to 0.40 mM in 24 h. The optimal temperature, pH, and initial OD_600 nm for ibuprofen transformation by strain D218 were 25-37 °C, 5.0-6.0, and 1.0-1.5, respectively. Strain D218 could simultaneously transform ibuprofen into the intermediates 2-hydroxyibuprofen and ibuprofen amide (IBUA). The two intermediates were further metabolized to 2-hydroxyibuprofen amide (2HIBUA), thus relieving the growth inhibition of ibuprofen in Scenedesmus obliquus. This is the first complete pathway reported for the detoxification of ibuprofen transformation by a Gram-positive strain. These findings further our understanding of the microbial catabolism of the IBU.

布洛芬是一种广泛使用的非甾体类消炎药，由于其经常被检测到且生物降解性差，它不仅污染农产品，还可能威胁人类健康。微生物代谢是消除环境中残留布洛芬的主要方式。菌株 D218 转化布洛芬的最佳温度、pH 值和初始 OD600 nm 分别为 25-37℃、5.0-6.0 和 1.0-1.5。菌株 D218 可同时将布洛芬转化为中间产物 2-羟基布洛芬和布洛芬酰胺（IBUA）。这两种中间产物可进一步代谢为 2-羟基布洛芬酰胺（2HIBUA），从而缓解布洛芬对双孢蘑菇生长的抑制作用。这是首次报道革兰氏阳性菌株对布洛芬转化进行解毒的完整途径。这些发现进一步加深了我们对 IBU 微生物分解代谢的了解。

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引用次数: 0