Pub Date : 2022-07-20DOI: 10.7324/jabb.2022.100501
Seetha Babu Manepalli, S. Tomar, Dinkar Gaikwad, S. Maitra
The plants are exposed seasonally and continuously to various environmental and biodiversity stresses that inhibit and affect their life processes from seedling to harvest stage. Several irregularities are seen in light intensity, temperature, mineral and water availability, etc. These changes keep on challenges the plant to grow and reproduce itself and produce several environmental signals. To receive these signals, the plants themselves develop a signaling network with several receptors such as phytohormones, G-protein-coupled receptors, kinases, and hormone receptors. Signal transduction produces a cellular response in plants which initiates the physiological and developmental responses. This article reveals a keen and in-depth analysis of several mechanisms and perceptions of signal transduction during exposure to several kinds of abiotic stresses in plants, along with a generic pathway of signaling in plants. Plant abiotic stress often plays a pivotal role in causing losses through salinity, heat, cold, drought, etc. To understand and overcome these problems through conventional breeding, which was mainly dependent on genetic variations, several studies are going on model plants such as Arabidopsis, rice, and Brachypodium ; the accessibility of sources for these genomes is in the processing stage in wheat. On the other hand, the advancements in genome editing opened the doors for scientists to incorporate the desired trait in a particular plant species. The emerging developments in the second-generation genome editing technologies like CRISPR/cas9 paved the path for plant biologists to develop a trait more efficiently and rapidly, unlike conventional breeding methods. This review plots the importance of signaling during abiotic stress and transgene technology to prevail over abiotic stress in plants by ingesting desired traits in a plant.
{"title":"Abiotic stress signaling in plants and transgenic technology as a triumph: A review","authors":"Seetha Babu Manepalli, S. Tomar, Dinkar Gaikwad, S. Maitra","doi":"10.7324/jabb.2022.100501","DOIUrl":"https://doi.org/10.7324/jabb.2022.100501","url":null,"abstract":"The plants are exposed seasonally and continuously to various environmental and biodiversity stresses that inhibit and affect their life processes from seedling to harvest stage. Several irregularities are seen in light intensity, temperature, mineral and water availability, etc. These changes keep on challenges the plant to grow and reproduce itself and produce several environmental signals. To receive these signals, the plants themselves develop a signaling network with several receptors such as phytohormones, G-protein-coupled receptors, kinases, and hormone receptors. Signal transduction produces a cellular response in plants which initiates the physiological and developmental responses. This article reveals a keen and in-depth analysis of several mechanisms and perceptions of signal transduction during exposure to several kinds of abiotic stresses in plants, along with a generic pathway of signaling in plants. Plant abiotic stress often plays a pivotal role in causing losses through salinity, heat, cold, drought, etc. To understand and overcome these problems through conventional breeding, which was mainly dependent on genetic variations, several studies are going on model plants such as Arabidopsis, rice, and Brachypodium ; the accessibility of sources for these genomes is in the processing stage in wheat. On the other hand, the advancements in genome editing opened the doors for scientists to incorporate the desired trait in a particular plant species. The emerging developments in the second-generation genome editing technologies like CRISPR/cas9 paved the path for plant biologists to develop a trait more efficiently and rapidly, unlike conventional breeding methods. This review plots the importance of signaling during abiotic stress and transgene technology to prevail over abiotic stress in plants by ingesting desired traits in a plant.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125353992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-20DOI: 10.7324/jabb.2022.100520
Darshankumar Prajapati, A. Bhatt, A. Gupte
The present study represents the purification and characterization of exopolysaccharide produced by Fomitopsis meliae AGDP-2. The water-soluble EPS obtained was purified using Sephadex G-75 column and characterized for its physical and biochemical properties. HPTLC and HPLC analysis indicated that EPS is composed of glucose and mannose residues. Many functional groups such as carboxyl, amide, hydroxyl, and glycosidic linkages were detected by FT-IR spectroscopy. Furthermore, glucose and mannose were detected from NMR spectra with α -(1→6) linkages, thereby signifying the heteropolymeric nature. Using GPC, molecular weight of EPS was estimated to be 2.48 × 10 6 Daltons. The SEM-EDX and AFM analysis revealed that EPS is compact and possesses cube-like structural units and nano-cracks with elemental composition of O, K, Na, Mg, S, Cl, and Ca. While studying its rheological behavior, EPS was found to be pseudoplastic in nature. As the physical properties and biochemical composition influence the bioactivities; the purified EPS from F. meliae AGDP-2 can be further evaluated for various bioactive properties such as antioxidant, anti-cancer, anti-inflammatory, antidiabetic, antibacterial, anti- biofilm, as well as antiviral properties. the present study was conducted to purify and characterize the EPS produced under submerged cultivation of Fomitopsis meliae AGDP-2. The characterization of EPS including monosaccharide composition (high-performance thin-layer chromatography [HPTLC] and HPLC), molecular weight (GPC), rheological behavior, surface morphology (SEM and AFM), as well as IR and nuclear magnetic resonance (NMR) spectra was thoroughly studied.
{"title":"Purification and physicochemical characterization of exopolysaccharide produced by a novel brown-rot fungus Fomitopsis meliae AGDP-2","authors":"Darshankumar Prajapati, A. Bhatt, A. Gupte","doi":"10.7324/jabb.2022.100520","DOIUrl":"https://doi.org/10.7324/jabb.2022.100520","url":null,"abstract":"The present study represents the purification and characterization of exopolysaccharide produced by Fomitopsis meliae AGDP-2. The water-soluble EPS obtained was purified using Sephadex G-75 column and characterized for its physical and biochemical properties. HPTLC and HPLC analysis indicated that EPS is composed of glucose and mannose residues. Many functional groups such as carboxyl, amide, hydroxyl, and glycosidic linkages were detected by FT-IR spectroscopy. Furthermore, glucose and mannose were detected from NMR spectra with α -(1→6) linkages, thereby signifying the heteropolymeric nature. Using GPC, molecular weight of EPS was estimated to be 2.48 × 10 6 Daltons. The SEM-EDX and AFM analysis revealed that EPS is compact and possesses cube-like structural units and nano-cracks with elemental composition of O, K, Na, Mg, S, Cl, and Ca. While studying its rheological behavior, EPS was found to be pseudoplastic in nature. As the physical properties and biochemical composition influence the bioactivities; the purified EPS from F. meliae AGDP-2 can be further evaluated for various bioactive properties such as antioxidant, anti-cancer, anti-inflammatory, antidiabetic, antibacterial, anti- biofilm, as well as antiviral properties. the present study was conducted to purify and characterize the EPS produced under submerged cultivation of Fomitopsis meliae AGDP-2. The characterization of EPS including monosaccharide composition (high-performance thin-layer chromatography [HPTLC] and HPLC), molecular weight (GPC), rheological behavior, surface morphology (SEM and AFM), as well as IR and nuclear magnetic resonance (NMR) spectra was thoroughly studied.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126785144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-20DOI: 10.7324/jabb.2022.100509
Subhasmita Panda, S. Swain, Rachita Sarangi
Scrub typhus is a vector-borne infectious disease caused by Orientia tsutsugamushi a gram-negative bacterium belonging to order Rickettsiales, endemic in Asia–Pacific region known as “Tsutsugamushi triangle.” This review aims to describe the seasonal outbreak of scrub typhus endemic in many places of India and in a global map which will be systematically analyze the current situation. The review focused on the diagnosis methods used to detect the disease also on the severity and complications during the clinical presentation. The data of scrub typhus outbreaks in different countries from 2000 to 2020 were retrieved from various sources. Gradually, increased case load and incidence rates have been recorded. The majority of the reported cases were from the North-East region of India during the cooler months. In Odisha, very few case reports were found, mostly from the capital, Bhubaneswar, but other areas have no records as it is still considered as an underreported disease. We concluded that India has experienced a large increase in scrub typhus incidence and documented an expansion in geographic distribution throughout the country. Therefore, people in rural areas need to be more conscious and aware to prevent the spread. Although no vaccine is available, several other preventive measures can be taken.
{"title":"An epidemiological outbreak of scrub typhus caused by Orientia tsutsugamushi – A comprehensive review","authors":"Subhasmita Panda, S. Swain, Rachita Sarangi","doi":"10.7324/jabb.2022.100509","DOIUrl":"https://doi.org/10.7324/jabb.2022.100509","url":null,"abstract":"Scrub typhus is a vector-borne infectious disease caused by Orientia tsutsugamushi a gram-negative bacterium belonging to order Rickettsiales, endemic in Asia–Pacific region known as “Tsutsugamushi triangle.” This review aims to describe the seasonal outbreak of scrub typhus endemic in many places of India and in a global map which will be systematically analyze the current situation. The review focused on the diagnosis methods used to detect the disease also on the severity and complications during the clinical presentation. The data of scrub typhus outbreaks in different countries from 2000 to 2020 were retrieved from various sources. Gradually, increased case load and incidence rates have been recorded. The majority of the reported cases were from the North-East region of India during the cooler months. In Odisha, very few case reports were found, mostly from the capital, Bhubaneswar, but other areas have no records as it is still considered as an underreported disease. We concluded that India has experienced a large increase in scrub typhus incidence and documented an expansion in geographic distribution throughout the country. Therefore, people in rural areas need to be more conscious and aware to prevent the spread. Although no vaccine is available, several other preventive measures can be taken.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115162273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-20DOI: 10.7324/jabb.2022.100524
V. Srivathsan, Mahima Bhandari, P. Swaminathan
Amylases are starch degrading enzymes. These enzymes play a pivotal role in the biotechnology industries in food, fermentation, textiles, and paper production. Many industries lack local amylase supply, so the demand for amylase is often high. The study focuses on the screening and isolation of amylase producing bacteria from disparate samples of soil. The promising hydrolytic strains of bacteria from the collected samples were characterized using enzyme kinetics and further investigation is carried out to characterize the amylase enzyme produced by optimization of pH and temperature. The shortlisted isolate was identified using 16s rRNA sequencing. This study is an initial result of an exploration where soil is a source of industrially important amylase producing bacterial strains.
{"title":"Isolation and characterization of starch degrading bacteria from disparate soil samples","authors":"V. Srivathsan, Mahima Bhandari, P. Swaminathan","doi":"10.7324/jabb.2022.100524","DOIUrl":"https://doi.org/10.7324/jabb.2022.100524","url":null,"abstract":"Amylases are starch degrading enzymes. These enzymes play a pivotal role in the biotechnology industries in food, fermentation, textiles, and paper production. Many industries lack local amylase supply, so the demand for amylase is often high. The study focuses on the screening and isolation of amylase producing bacteria from disparate samples of soil. The promising hydrolytic strains of bacteria from the collected samples were characterized using enzyme kinetics and further investigation is carried out to characterize the amylase enzyme produced by optimization of pH and temperature. The shortlisted isolate was identified using 16s rRNA sequencing. This study is an initial result of an exploration where soil is a source of industrially important amylase producing bacterial strains.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125673986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-20DOI: 10.7324/jabb.2022.100511
Shilpi Singh, Kamlesh Choure, P. Rai, S. Gour, V. Agnihotri
The study was conducted with an aim of isolating and identifying bacterial endophytes associated with Musa acuminata and assessing their plant growth-promoting properties. Endophytic bacteria show mutualistic relationship with plants and help them in alleviating several biotic and abiotic stress without showing any apparent negative effect to the host plant. In the present study, explants samples from different parts of M. acuminata plant such as root, stem, and leaves were collected and cultured. A total of 33 bacterial isolates were obtained and screened for their biotechnological potential for promoting plant growth. From which, 19 isolates were selected for further analysis based on their in vitro plant growth-promoting activities that include indole-3-acetic acid production, phosphate solubilization, nitrogen fixation, ammonia production, hydrogen cyanide (HCN) production, and siderophore production. In addition, these isolates also evaluated for the antagonist activity against Fusarium oxysporum and Macrophomina phaseolina. Among them, five isolates were sequenced, on the basis of 16S rRNA gene sequencing homology of the representative strains was identified EMS1 and EMS4 as Bacillus cereus , EMS13, 14, and 18 as Enterobacter cloacae, and EMS16 as Enterobacter hormaechei. Phylogenetic tree indicated evolutionary relationship of these bacteria to their closely related species. The result of this study demonstrated that based on growth-promoting competencies, all isolated strains have ability that influence the growth of host plants and have potential to be used as effective growth promoting bioinoculant for M. acuminata .
{"title":"Evaluation of plant growth-promoting activities of endophytic bacteria of Musa acuminata and their characterization","authors":"Shilpi Singh, Kamlesh Choure, P. Rai, S. Gour, V. Agnihotri","doi":"10.7324/jabb.2022.100511","DOIUrl":"https://doi.org/10.7324/jabb.2022.100511","url":null,"abstract":"The study was conducted with an aim of isolating and identifying bacterial endophytes associated with Musa acuminata and assessing their plant growth-promoting properties. Endophytic bacteria show mutualistic relationship with plants and help them in alleviating several biotic and abiotic stress without showing any apparent negative effect to the host plant. In the present study, explants samples from different parts of M. acuminata plant such as root, stem, and leaves were collected and cultured. A total of 33 bacterial isolates were obtained and screened for their biotechnological potential for promoting plant growth. From which, 19 isolates were selected for further analysis based on their in vitro plant growth-promoting activities that include indole-3-acetic acid production, phosphate solubilization, nitrogen fixation, ammonia production, hydrogen cyanide (HCN) production, and siderophore production. In addition, these isolates also evaluated for the antagonist activity against Fusarium oxysporum and Macrophomina phaseolina. Among them, five isolates were sequenced, on the basis of 16S rRNA gene sequencing homology of the representative strains was identified EMS1 and EMS4 as Bacillus cereus , EMS13, 14, and 18 as Enterobacter cloacae, and EMS16 as Enterobacter hormaechei. Phylogenetic tree indicated evolutionary relationship of these bacteria to their closely related species. The result of this study demonstrated that based on growth-promoting competencies, all isolated strains have ability that influence the growth of host plants and have potential to be used as effective growth promoting bioinoculant for M. acuminata .","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124967150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20DOI: 10.7324/jabb.2022.10s205
Manali Singh, K. Jayant, Anshi Mehra, Shivani Bhutani, T. Kaur, Divjot Kour, D. Suyal, Sangram Singh, A. Rai, Ajar Nath Yadav
{"title":"Bioremediation— sustainable tool for diverse contaminants management: Current scenario and future aspects","authors":"Manali Singh, K. Jayant, Anshi Mehra, Shivani Bhutani, T. Kaur, Divjot Kour, D. Suyal, Sangram Singh, A. Rai, Ajar Nath Yadav","doi":"10.7324/jabb.2022.10s205","DOIUrl":"https://doi.org/10.7324/jabb.2022.10s205","url":null,"abstract":"","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124017444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20DOI: 10.7324/jabb.2022.10s208
R. Prabha, V. Nigam
The reduction of non-renewable material has raised significant concerns for several years over the sustainable production of organic acids through bio-based methods in the world. One such way to overcome this problem is to use reactive extractants, in which appropriate extractants are employed to recover various organic and inorganic acids. The extraction of acrylic acid by solvent extraction is an illustration of this technique. The current study focuses on the synthesis of acrylic acid from acrylamidase produced by Bacillus tequilensis , succeeded by acid extraction from the amidase-catalyzed reaction by a solvent technique. Among the various solvents, ethyl acetate (2:1, v/v) was established as the most appropriate solvent for the extraction. Exactly 65 mg of raw acrylic acid was recovered from 20 ml of the amidase-catalyzed reaction. Various analytical methods such as thin layer chromatography, fourier transform infrared spectroscopy, high-performance liquid chromatography (HPLC), and mass spectrophotometry were accomplished for the identification, validation, and quantification of the extracted acrylic acid. The m / z value of acrylic acid obtained in the extracted product was 73.18, which was similar to the standard acrylic acid. From HPLC, almost 34% of bioconversion was quantified (3.4 mM) from 10 mM of acrylamide consumed. The extracted acrylic acid can be further exploited as chemical intermediates and pharmaceuticals in the future.
{"title":"Extraction and quantification of acrylic acid from acrylamidase-catalyzed reaction produced by Bacillus tequilensis","authors":"R. Prabha, V. Nigam","doi":"10.7324/jabb.2022.10s208","DOIUrl":"https://doi.org/10.7324/jabb.2022.10s208","url":null,"abstract":"The reduction of non-renewable material has raised significant concerns for several years over the sustainable production of organic acids through bio-based methods in the world. One such way to overcome this problem is to use reactive extractants, in which appropriate extractants are employed to recover various organic and inorganic acids. The extraction of acrylic acid by solvent extraction is an illustration of this technique. The current study focuses on the synthesis of acrylic acid from acrylamidase produced by Bacillus tequilensis , succeeded by acid extraction from the amidase-catalyzed reaction by a solvent technique. Among the various solvents, ethyl acetate (2:1, v/v) was established as the most appropriate solvent for the extraction. Exactly 65 mg of raw acrylic acid was recovered from 20 ml of the amidase-catalyzed reaction. Various analytical methods such as thin layer chromatography, fourier transform infrared spectroscopy, high-performance liquid chromatography (HPLC), and mass spectrophotometry were accomplished for the identification, validation, and quantification of the extracted acrylic acid. The m / z value of acrylic acid obtained in the extracted product was 73.18, which was similar to the standard acrylic acid. From HPLC, almost 34% of bioconversion was quantified (3.4 mM) from 10 mM of acrylamide consumed. The extracted acrylic acid can be further exploited as chemical intermediates and pharmaceuticals in the future.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"145 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115786229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20DOI: 10.7324/jabb.2022.10s201
Ajar Nath Yadav, D. Suyal, Divjot Kour, V. Rajput, A. Rastegari, Joginder Singh
gricultural practices, industrial manufacturing, and lifestyle of human beings have raised the accumulation of hazardous compounds into the surrounding. It has drastically increased health issues and environmental concerns. Therefore, there is an urgent need for eco-friendly approaches to solving these multiple issues at a time. Contrary to the harmful chemical methods, bioremediation has emerged as an eco-friendly substitute for sustainable development. Bioremediation utilizes living microbiomes for cleaning environment and their sustainability. As advancement in science and technology, researchers have developed various bioremediation techniques but because of nature and type of pollutant, there is no single ‘silver bullet’ which can be applied to restore the polluted environment. The ever-increasing population and our reckless acts are turning the Earth into a huge garbage dump. The indiscriminate exploitation of resources making this condition worsen. Moreover, it is a major cause of climatic irregularities, environmental destructions, biodiversity shrinking, disease outbreaks, and agricultural issues. The present waste management efforts are proving insufficient to handle this situation.
{"title":"Bioremediation and Waste Management for Environmental Sustainability","authors":"Ajar Nath Yadav, D. Suyal, Divjot Kour, V. Rajput, A. Rastegari, Joginder Singh","doi":"10.7324/jabb.2022.10s201","DOIUrl":"https://doi.org/10.7324/jabb.2022.10s201","url":null,"abstract":"gricultural practices, industrial manufacturing, and lifestyle of human beings have raised the accumulation of hazardous compounds into the surrounding. It has drastically increased health issues and environmental concerns. Therefore, there is an urgent need for eco-friendly approaches to solving these multiple issues at a time. Contrary to the harmful chemical methods, bioremediation has emerged as an eco-friendly substitute for sustainable development. Bioremediation utilizes living microbiomes for cleaning environment and their sustainability. As advancement in science and technology, researchers have developed various bioremediation techniques but because of nature and type of pollutant, there is no single ‘silver bullet’ which can be applied to restore the polluted environment. The ever-increasing population and our reckless acts are turning the Earth into a huge garbage dump. The indiscriminate exploitation of resources making this condition worsen. Moreover, it is a major cause of climatic irregularities, environmental destructions, biodiversity shrinking, disease outbreaks, and agricultural issues. The present waste management efforts are proving insufficient to handle this situation.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121204551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20DOI: 10.7324/jabb.2022.10s209
Anu Kumar, S. Shivani, B. Krishan, M. Samtiya, T. Dhewa
Industrialization plays a major role in strengthening the economy of any country. However, these industries directly or indirectly affect the environment. Industrial wastewater discharge has been reported with certain heavy metals such as chromium, lead, cobalt, and others which are a potential hazard to the water bodies and humans as well. Biofilm is an applied method in the fields of bioremediation for reliving this emerging problem and in the efficient removal of heavy metals from wastewater. Biofilms of Escherichia coli and petroleum soil isolated microorganisms ( PSIM ) were developed at the V bottom of 96 well microtiter plate. The contaminated water sample was collected from the textile industry, Solan, Himachal Pradesh, India. The biofilms were incubated with the industrial water tested for heavy metals assuming the microbes have the potential to assimilate the heavy metals up to 5 mg/mL of concentration. After the incubation for 1–2 weeks, the microorganisms were able to reduce the level of heavy metals present in the samples which was conveyed by biomass comparison of microorganisms in the successive intervals of time. 0.74 × 10 10 cells/mL and 0.77 × 10 10 cell/mL of E. coli and PSIM biofilms were able to tolerate the metal toxicity on incubation for 2 weeks at the highest concentration due to the functional group present extracellular polymeric substance which forms complexes with heavy metals. This leads to the fact that these biofilms have assimilated the heavy metals and are potent for the removal of heavy metals from industrial wastewater.
{"title":"Ex-situ biofilm mediated approach for bioremediation of selected heavy metals in wastewater of textile industry","authors":"Anu Kumar, S. Shivani, B. Krishan, M. Samtiya, T. Dhewa","doi":"10.7324/jabb.2022.10s209","DOIUrl":"https://doi.org/10.7324/jabb.2022.10s209","url":null,"abstract":"Industrialization plays a major role in strengthening the economy of any country. However, these industries directly or indirectly affect the environment. Industrial wastewater discharge has been reported with certain heavy metals such as chromium, lead, cobalt, and others which are a potential hazard to the water bodies and humans as well. Biofilm is an applied method in the fields of bioremediation for reliving this emerging problem and in the efficient removal of heavy metals from wastewater. Biofilms of Escherichia coli and petroleum soil isolated microorganisms ( PSIM ) were developed at the V bottom of 96 well microtiter plate. The contaminated water sample was collected from the textile industry, Solan, Himachal Pradesh, India. The biofilms were incubated with the industrial water tested for heavy metals assuming the microbes have the potential to assimilate the heavy metals up to 5 mg/mL of concentration. After the incubation for 1–2 weeks, the microorganisms were able to reduce the level of heavy metals present in the samples which was conveyed by biomass comparison of microorganisms in the successive intervals of time. 0.74 × 10 10 cells/mL and 0.77 × 10 10 cell/mL of E. coli and PSIM biofilms were able to tolerate the metal toxicity on incubation for 2 weeks at the highest concentration due to the functional group present extracellular polymeric substance which forms complexes with heavy metals. This leads to the fact that these biofilms have assimilated the heavy metals and are potent for the removal of heavy metals from industrial wastewater.","PeriodicalId":423079,"journal":{"name":"Journal of Applied Biology & Biotechnology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131204884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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