Ultrasonics Sonochemistry最新文献_第8页

Theoretical and experimental research on the impacts of the Joukowsky hydrofoils on the bubble collapse dynamics within a confined space 关于焦科夫斯基水翼对密闭空间内气泡坍塌动力学影响的理论和实验研究

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-24 DOI: 10.1016/j.ultsonch.2024.107174

Junwei Shen , Hongbo Wang , Cheng Zhang , Yuning Zhang , Yuning Zhang

The present paper investigates the bubble collapse dynamics near hydrofoils within a confined space. Experiments involving high-speed photography reveal in detail the typical bubble morphological evolution near different hydrofoils between two glass plates (namely the confined space), and the partitioning of the thickness-related and camber-related parameters is analyzed quantitatively. Based on conformal transformation, the liquid velocity field and Kelvin impulse are used to analyze the bubble collapse characteristics qualitatively and quantitatively, including the bubble interface motion, cross-sectional roundness, and collapse jet. The main conclusions are summarized as follows. (1) The bubble morphological evolution near the hydrofoils can be categorized into five typical collapse shapes, and their partition ranges are significantly affected by the thickness-related and camber-related parameters. (2) The thickness-related hydrofoil parameter positively correlated with the bubble interface motion and cross-sectional roundness, while the camber-related parameter is inversely correlated with them. (3) High-velocity regions between the bubble and the hydrofoil head and tail endpoints explain the bubble interface depressions observed in the experiments

本文研究了密闭空间内水翼附近的气泡坍塌动力学。高速摄影实验详细揭示了两块玻璃板（即密闭空间）之间不同水翼附近典型的气泡形态演变，并定量分析了厚度相关参数和凸度相关参数的分区。在保角变换的基础上，利用液体速度场和开尔文冲量定性和定量分析了气泡塌陷特征，包括气泡界面运动、横截面圆度和塌陷射流。主要结论归纳如下。(1) 水翼附近的气泡形态演变可分为五种典型的塌陷形状，其分区范围受厚度相关参数和凸度相关参数的显著影响。(2）与厚度相关的水翼参数与气泡界面运动和横截面圆度呈正相关，而与凸度相关的参数与之呈反相关。(3) 气泡与水翼头尾端点之间的高速区域解释了实验中观察到的气泡界面凹陷现象

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引用次数: 0

Ultrasonication modifies the structural, thermal and functional properties of pumpkin seed protein isolate (PSPI) 超声波改变南瓜籽分离蛋白（PSPI）的结构、热和功能特性

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-22 DOI: 10.1016/j.ultsonch.2024.107172

Mehvish Habib , Sakshi Singh , Sameer Ahmad , Shumaila Jan , Ankit Gupta , Kulsum Jan , Khalid Bashir

Protein isolates from pumpkin seeds were prepared and then treated with high-intensity ultrasound (HIUS) using a probe-based method. The impact of ultrasonication on the physicochemical, molecular, and thermal properties of these isolates were analyzed and compared to untreated controls. Results showed significant improvements (p ≤ 0.05) in color (L*, a*, b* values), solubility, emulsification capacity, and stability, as well as a reduction in molecular weight, indicating enhanced functionality of the pumpkin seed protein isolates (PSPIs) after HIUS treatment. However, HIUS treatment decreased the denaturation temperature (Td), denaturation enthalpy (ΔH), thermal stability, and particle size of the isolates. With treatment durations ranging from 5 to 20 min, T_d dropped from 67.31 °C to 56.38 °C, and ΔH declined from 45.78 to 35.43 J/g, likely due to structural and conformational modifications from ultrasonic-induced molecular bond disruptions. The greatest reduction in particle size, from 117.46 μm to 85.26 μm, was observed after 20 min of ultrasonication. X-ray diffraction (XRD) analysis showed two distinct diffraction peaks at 2θ = 10° and 2θ = 20°, indicating altered crystallite sizes post-ultrasound treatment. Ultrasonication induced structural and conformational changes in the pumpkin seed protein isolates, as confirmed by SDS-PAGE and weight loss analyses. Alterations in the SDS-PAGE profile and reduced weight loss were associated with improved solubility and enhanced thermal and functional properties in the treated pumpkin seed protein isolates. This emphasizes the potential of PSPI to increase their value-added potential through ultrasonication.

制备南瓜籽蛋白质分离物，然后用探针法进行高强度超声（HIUS）处理。分析了超声处理对这些分离物的理化、分子和热特性的影响，并与未经处理的对照组进行了比较。结果表明，经 HIUS 处理后，南瓜籽蛋白质分离物（PSPIs）的色泽（L*、a*、b* 值）、溶解度、乳化能力和稳定性均有明显改善（p ≤ 0.05），分子量也有所降低，这表明南瓜籽蛋白质分离物的功能得到了增强。然而，HIUS 处理降低了分离物的变性温度（Td）、变性焓（ΔH）、热稳定性和粒度。处理持续时间从 5 分钟到 20 分钟不等，Td 从 67.31 °C 下降到 56.38 °C，ΔH 从 45.78 焦耳/克下降到 35.43 焦耳/克，这可能是由于超声波引起的分子键破坏导致了结构和构象的改变。在超声处理 20 分钟后，观察到粒径从 117.46 微米降至 85.26 微米，降幅最大。X 射线衍射 (XRD) 分析显示，在 2θ = 10° 和 2θ = 20° 处有两个不同的衍射峰，表明超声处理后晶体尺寸发生了变化。经 SDS-PAGE 和失重分析证实，超声波处理可诱导南瓜籽蛋白质分离物的结构和构象发生变化。SDS-PAGE 分析结果的改变和重量损失的减少与经处理的南瓜籽蛋白质分离物溶解度的提高以及热性能和功能特性的增强有关。这强调了南瓜籽蛋白质分离物通过超声波处理提高其增值潜力的潜力。

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引用次数: 0

Hydrogel carrier with bubble vibration enhancer for ultrasound-triggered drug release 带有气泡振动增强器的水凝胶载体，用于超声波触发的药物释放

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-22 DOI: 10.1016/j.ultsonch.2024.107173

Ryuto Yamakawa , Hiroaki Onoe , Yuta Kurashina

Hydrogel-based drug carriers provide on-demand drug release via external stimuli. Ultrasound is a promising method because of the potential for remotely releasing the drug. However, intense ultrasound irradiation has been required in previous studies. This paper reports drug model release from hydrogel carriers encapsulating bubble vibration enhancers (BVEs) consisting of microbubbles coated with a lipid membrane. Vibration of BVEs induced by ultrasound stimulation promoted the release of drug models with ultrasound irradiation controlled to a biologically safe acoustic pressure based on spatial-peak temporal-average intensity (I_SPTA). The release ratio increased significantly from 2.3 % without BVEs and ultrasound to 10.2 % with both. To evaluate the frequency response, the release ratio was measured at three different ultrasound frequencies (0.3, 1.8, and 2.5 MHz), showing increased efficiency as the frequency approached the resonance frequency of the BVEs. For in vivo applications, hydrogel microspherical carriers with BVEs achieved a 12 % release ratio. Poly-L-lysine coating successfully suppressed the drug release to 0.2 %. The carriers demonstrated repeated responsiveness when ultrasound was applied in three 5-minute intervals. The hydrogel carrier encapsulating BVEs we proposed is a promising in vivo device capable of releasing drugs on demand by ultrasound irradiation based on its high biosafety and acoustic responsiveness.

水凝胶药物载体可通过外部刺激按需释放药物。超声波是一种很有前景的方法，因为它具有远程释放药物的潜力。然而，以往的研究需要强烈的超声波照射。本文报告了水凝胶载体包裹气泡振动增强剂（BVEs）的药物释放模型，气泡振动增强剂由涂有脂质膜的微气泡组成。根据空间-峰值-时间-平均强度（ISPTA）将超声辐照控制在生物安全声压范围内，超声刺激诱导的 BVEs 振动促进了药物模型的释放。药物释放率从没有 BVE 和超声的 2.3% 显著增加到有 BVE 和超声的 10.2%。为了评估频率响应，在三种不同的超声频率（0.3、1.8 和 2.5 兆赫）下测量了释放率，结果表明，当频率接近 BVE 的共振频率时，释放率会提高。在体内应用中，带有 BVE 的水凝胶微球载体达到了 12% 的释放率。聚 L-赖氨酸涂层成功地将药物释放率抑制到了 0.2%。当超声波以三个 5 分钟的时间间隔施加时，载体表现出重复响应性。我们提出的包裹 BVE 的水凝胶载体具有很高的生物安全性和声学响应性，是一种很有前途的体内装置，能够通过超声照射按需释放药物。

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引用次数: 0

Effect of driving frequency and power on droplet size atomized by a multimodal transducer 驱动频率和功率对多模态传感器雾化液滴大小的影响

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.ultsonch.2024.107166

Weiquan Wang , Zhirui Chen , Hiroshi Hasegawa , Kohsuke Hirano , Chikahiro Imashiro , Takeshi Morita

Ultrasonic atomization is used in various applications such as medical devices, material synthesis, and humidity control. To meet the needs of different applications, the required droplet size ranges from a few microns to several hundred microns. In this study, we realized wideband multiple-frequency atomization with a multimodal transducer, which could control the size of atomized droplets by switching operating frequencies. A parabolic reflector served to focus the incident ultrasound waves, and a circular plate was employed to excite bending modes. By comparing the results of frequency response experiments with those of finite element simulation, nine different bending modes were acquired in the range of 500–2500 kHz. Then, 734, 949, 1530, and 2063 kHz were selected to examine atomization performance for producing 1–10 μm water droplets. The relationship between the droplet diameter and the operating frequency was clarified. The droplet diameter was found to follow a log-normal distribution. At these four frequencies, the mean droplet diameter was 7.71, 6.57, 4.87, and 4.43 μm, respectively, meaning that the diameter decreased with increasing frequency. The mean droplet diameter decreased with increasing input power at 734 kHz, whereas at 2063 kHz, no significant trend was shown. Furthermore, the atomization rate was found to increase at higher power and exhibited a frequency dependence. These results indicated that the multimodal transducer has promising potential for high-frequency ultrasonic atomization and the capacity to control the droplet size.

超声波雾化技术应用广泛，如医疗器械、材料合成和湿度控制等。为满足不同应用的需要，所需的液滴尺寸从几微米到几百微米不等。在这项研究中，我们利用多模态换能器实现了宽带多频雾化，通过切换工作频率来控制雾化液滴的大小。抛物面反射器用于聚焦入射超声波，圆板用于激发弯曲模式。通过比较频率响应实验结果和有限元模拟结果，在 500-2500 kHz 范围内获得了九种不同的弯曲模式。然后，选择 734、949、1530 和 2063 kHz 来检测产生 1-10 μm 水滴的雾化性能。液滴直径与工作频率之间的关系得到了明确。研究发现，水滴直径呈对数正态分布。在这四种频率下，水滴的平均直径分别为 7.71、6.57、4.87 和 4.43 μm，这意味着水滴直径随着频率的增加而减小。在频率为 734 kHz 时，平均液滴直径随输入功率的增加而减小，而在频率为 2063 kHz 时，则没有明显的趋势。此外，还发现雾化率在较高功率下会增加，并表现出频率依赖性。这些结果表明，多模态换能器在高频超声雾化和控制液滴大小方面具有良好的潜力。

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引用次数: 0

Effect of ultrasound on the functional properties and structural changes of chicken liver insoluble proteins isolated by isoelectric solubilization/precipitation 超声波对等电位溶解/沉淀法分离的鸡肝不溶性蛋白质的功能特性和结构变化的影响

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.ultsonch.2024.107165

Rongrong Mao , Guoyuan Xiong , Haibo Zheng , Jun Qi , Chunhui Zhang

The studies investigated the effects of different ultrasonic powers (180, 360 and 540 W) on the functional properties and structural changes of chicken liver insoluble proteins (CLIPs) isolated by isoelectric solubilization/precipitation (ISP) (with alkaline solubilization at pH 11.0 and pH 12.0 respectively, and acid precipitation at pH 5.5). Results indicated that ultrasonic significantly increased the solubility of ISP-isolated CLIPs, and narrowed the particle size distribution of D₃,₂ and D₄,₃ (P < 0.05). The highest solubility was observed at pH 11.0 and 360 W ultrasound treatment, reaching 77.26 %. The ultrasonic with 360 W exhibited higher shear stress and apparent viscosity. Spectroscopy revealed that compared to without ultrasonic treatment, there was an increase in β-sheet and random curling content accompanied by a decrease in β-turn and α-helix structure when ultrasonication. Ultrasound altered the tyrosine hydrophobic residues to be exposed to the surface of the ISP-isolated CLIPs, thus improving the hydrophilicity. Overall, ultrasound combined with ISP treatment effectively improved the functional properties of CLIPs, and it might be a potential, safe and efficient method for improving the processing properties and broadening the application of insoluble animal-derived proteins.

研究探讨了不同超声功率（180、360 和 540 W）对等电溶解/沉淀法（ISP）分离的鸡肝不溶性蛋白（CLIPs）（分别在 pH 11.0 和 pH 12.0 下进行碱溶解，在 pH 5.5 下进行酸沉淀）的功能特性和结构变化的影响。结果表明，超声波能显著提高 ISP 分离出的 CLIP 的溶解度，并缩小 D3,2 和 D4,3 的粒度分布（P <0.05）。在 pH 值为 11.0、超声处理功率为 360 W 时，溶解度最高，达到 77.26%。360 瓦的超声波具有更高的剪切应力和表观粘度。光谱分析显示，与不使用超声波处理相比，超声波处理时β-片状结构和无规卷曲结构含量增加，而β-匝状结构和α-螺旋结构含量减少。超声改变了酪氨酸疏水残基，使其暴露于 ISP 分离的 CLIPs 表面，从而提高了亲水性。总之，超声结合ISP处理可有效改善CLIPs的功能特性，它可能是一种潜在的、安全的、高效的方法，可用于改善不溶性动物源性蛋白质的加工性能并拓宽其应用领域。

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引用次数: 0

Advancements in Acoustic Cavitation Modelling: Progress, Challenges, and Future Directions in Sonochemical Reactor Design 声空化建模的进展：声化学反应器设计的进展、挑战和未来方向

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.ultsonch.2024.107163

T.Joyce Tiong , Jin Kiat Chu , Khang Wei Tan

This review provides a comprehensive overview of ultrasonic wave propagation, with a primary focus on high-power ultrasound systems where cavitation bubbles are likely to occur. The review is structured to guide readers through the historical development of cavitation models, from early works such as the Rayleigh-Plesset equation to more advanced numerical approaches. It explores the dynamics of cavitation bubbles, their physical effects, and the key factors influencing bubble formation, growth, and collapse. In addition to bubble-induced cavitation, the review addresses nonlinear wave propagation in the absence of bubbles, highlighting phenomena such as harmonic generation and shock wave formation. A detailed discussion on the numerical modelling of ultrasonic systems follows, covering linear and nonlinear approaches, boundary conditions, and the challenges of accurately simulating cavitating systems. The review concludes with an analysis of recent developments, emerging trends, and future directions in computational modelling for ultrasonic applications. By presenting a structured overview of both the theoretical and practical aspects of ultrasonic wave propagation, this work aims to provide a foundation for future research and design improvements in sonochemical and acoustic systems.

这篇综述提供了超声波传播的全面概述，主要集中在可能发生空化气泡的高功率超声系统上。回顾的结构是引导读者通过空化模型的历史发展，从早期的作品，如瑞利-普莱塞特方程更先进的数值方法。它探讨了空化气泡的动力学，它们的物理效应，以及影响气泡形成，生长和崩溃的关键因素。除了气泡引起的空化外，该综述还讨论了在没有气泡的情况下的非线性波传播，突出了谐波产生和激波形成等现象。详细讨论了超声系统的数值模拟，包括线性和非线性方法，边界条件，以及准确模拟空化系统的挑战。本文最后分析了超声应用计算模型的最新发展、新趋势和未来方向。通过对超声波传播的理论和实践方面进行结构化的概述，本工作旨在为声化学和声学系统的未来研究和设计改进提供基础。

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引用次数: 0

Study on optimization of extraction and purification processes for total flavonoids from Lycopi herba roots and their anti-proliferative effects on fibrous synoviocytes in human rheumatoid arthritis 从枸杞根中提取和纯化总黄酮及其对人类类风湿性关节炎纤维滑膜细胞抗增殖作用的优化工艺研究

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.ultsonch.2024.107164

Jie Chen , Chunyang Jiang , Zhenyu Liu , Panpan Wang , Qiang Ma , Nong Zhou

Lycopi herba, a traditional Chinese medicinal plant, has long been valued for its aerial parts. however, its roots, which are often discarded as non-medicinal waste, actually contain flavonoid compounds that possess potential medicinal values such as anti-inflammatory, antioxidant, and anti-tumor activities. Despite this, studies on the extraction, purification, and biological activity assessment of total flavonoids from L. herba roots (TFLHR) remain inadequate. Our study aimed to optimize the extraction and purification processes for TFLHR and evaluate their anti-proliferative effects on human fibroblast-like synoviocytes (HFLS-RA), which are key pathological cells in rheumatoid arthritis. By utilizing ultrasound-assisted extraction combined with response surface methodology (RSM), we optimized the extraction conditions, achieving a total flavonoid content of 90.484 ± 0.974 mg/g under the optimal settings: a liquid–solid ratio of 48:1 mL/g, 13 min of ultrasound treatment, 70 % ethanol, and an extraction temperature of 43°C. Subsequently, macroporous resin chromatography was employed for flavonoid purification, with AB-8 resin exhibiting the highest performance, achieving adsorption and desorption rates of 79.64 ± 1.51 % and 88.61 ± 1.02 %, respectively. By further refining the purification conditions through RSM, the purity of flavonoids was increased to 63.9 ± 1.86 %. Through ultra performance liquid chromatography tandem-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) analysis, 74 flavonoid compounds across 15 categories were identified. Further activity studies demonstrated that purified TFLHR exhibited significant concentration-dependent anti-proliferative effects on HFLS-RA cells. This study not only provides a scientific basis for the comprehensive utilization of L. herba root resources but also highlights the potential medicinal value of TFLHR in the treatment of rheumatoid arthritis, laying a foundation for future research into its specific mechanisms and clinical applications.

枸杞是一种传统的中药植物，长期以来其气生部分一直受到人们的重视。然而，枸杞根（通常作为非药用废物丢弃）实际上含有黄酮类化合物，具有潜在的药用价值，如抗炎、抗氧化和抗肿瘤活性。尽管如此，有关茜草根（TFLHR）中总黄酮类化合物的提取、纯化和生物活性评估的研究仍然不足。我们的研究旨在优化 TFLHR 的提取和纯化过程，并评估其对类风湿关节炎的关键病理细胞--人成纤维细胞样滑膜细胞（HFLS-RA）的抗增殖作用。我们利用超声辅助萃取结合响应面方法（RSM）优化了萃取条件，在液固比48:1 mL/g、超声处理13分钟、70%乙醇、萃取温度43°C的最佳设置下，总黄酮含量达到90.484 ± 0.974 mg/g。随后，采用大孔树脂色谱法纯化黄酮类化合物，其中 AB-8 树脂的性能最高，吸附率和解吸率分别为 79.64 ± 1.51 % 和 88.61 ± 1.02 %。通过 RSM 进一步改进纯化条件，黄酮类化合物的纯度提高到 63.9 ± 1.86 %。通过超高效液相色谱-串联四极杆飞行时间串联质谱（UPLC-Q-TOF-MS/MS）分析，共鉴定出 15 个类别的 74 种黄酮类化合物。进一步的活性研究表明，纯化的 TFLHR 对 HFLS-RA 细胞具有显著的浓度依赖性抗增殖作用。这项研究不仅为枸杞根资源的综合利用提供了科学依据，而且凸显了TFLHR在治疗类风湿性关节炎方面的潜在药用价值，为今后研究其具体机制和临床应用奠定了基础。

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引用次数: 0

Experimental and numerical research on jet dynamics of cavitation bubble near dual particles 双颗粒附近空化气泡射流动力学的实验和数值研究

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.ultsonch.2024.107168

Yuning Zhang , Xuan Lu , Jinsen Hu , Jiaxin Yu , Yuning Zhang

The current paper delves into the jet dynamics arising from a cavitation bubble in proximity to a dual-particle system, employing both experimental methodology and numerical simulation. The morphological development of a laser-induced bubble as well as the production of jets are captured by utilizing high-speed photography. The principles of bubble morphology evolution and jet formation are revealed by a OpenFOAM solver, which takes into account the effects of two-phase fluid compressibility, phase changes, heat transfer, and surface tension. Fluid temperature variations induced by bubble oscillations are discussed. The results indicate that the jet dynamics can be categorized into three cases, i.e. bubble-splitting double jets, impacting single jet, non-impacting double jets. For bubble-splitting double jets, bubble splitting is induced by an annular pressure gradient towards the bubble axis. This resulted in the production of two unequal-sized sub-bubbles, which subsequently produced double jets in opposite directions. The fluid temperature close to the bubble interface is low, while the bubble center is high. For impacting single jet, it is induced by a conical pressure gradient towards the nearest particle and the jet impacts the particle. The fluid temperature is low near the jet and high near the particle. When the jet penetrates the bubble interface, the temperature inside the bubble reaches its peak. For non-impacting double jets, they are induced by pressure gradients facing each other and they do not impact particles. The temperature inside the bubble increases with the proximity of the two jets.

本文采用实验方法和数值模拟，深入研究了双粒子系统附近的空化气泡产生的喷流动力学。通过高速摄影捕捉了激光诱导气泡的形态发展以及喷流的产生。气泡形态演变和喷流形成的原理由 OpenFOAM 求解器揭示，该求解器考虑了两相流体可压缩性、相变、传热和表面张力的影响。讨论了气泡振荡引起的流体温度变化。结果表明，射流动力学可分为三种情况，即气泡分裂双射流、冲击单射流和非冲击双射流。对于气泡分裂双射流，气泡分裂是由朝向气泡轴的环形压力梯度引起的。这导致产生两个大小不等的子气泡，随后产生方向相反的双射流。靠近气泡界面的流体温度较低，而气泡中心的温度较高。对于撞击单射流，它是由朝向最近颗粒的锥形压力梯度引起的，射流撞击颗粒。射流附近的流体温度较低，而粒子附近的流体温度较高。当射流穿透气泡界面时，气泡内的温度达到峰值。对于非撞击型双射流，它们是由相互对峙的压力梯度引起的，不会撞击颗粒。气泡内的温度随着两个射流的靠近而升高。

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引用次数: 0

Research on the dynamic characteristics of the cavitation bubble collapsing between multiple particles 多颗粒间空化气泡坍塌的动态特性研究

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-19 DOI: 10.1016/j.ultsonch.2024.107169

Xiaoyu Wang , Jingrong Hu , Yufei Wang , Yuning Zhang , Yuning Zhang

The combined action of multiple particles and cavitation bubbles can severely damage hydraulic machinery. Combining the Kelvin impulse theory and the results of high-speed photography experiments, this paper researches the dynamic behaviors of a single bubble located between three equal-sized spherical particles. Non-spherical morphological evolution characteristics in the collapse stage of the bubble are described. The influence of the arrangement of the three particles on the direction and intensity of the bubble migration is quantitatively analyzed. On this basis, the spatial distribution characteristics of the zero impulse points with the Kelvin impulse equal to zero are explored. The results show that: (1) As the bubble is induced in the symmetric positions, three typical cases of collapse characteristics are summarized according to the bubble morphology, including V-shaped, T-shaped, and ginkgo leaf-shaped. (2) As the bubble incipient position is shifted on the symmetry axis, the Kelvin impulse intensity shows a non-monotonic trend with its direction varying many times. Both the impulse intensity and direction are significantly affected by the arrangement of particles. (3) There are multiple zero impulse points among the three particles, and both the spatial location and the number of the zero impulse points are affected by the arrangement of the particles.

多个颗粒和空化气泡的共同作用会严重损坏液压机械。本文结合开尔文脉冲理论和高速摄影实验结果，研究了位于三个大小相等的球形颗粒之间的单个气泡的动态行为。描述了气泡坍塌阶段的非球形形态演变特征。定量分析了三个颗粒的排列对气泡迁移方向和强度的影响。在此基础上，探讨了开尔文脉冲等于零时零脉冲点的空间分布特征。结果表明(1) 气泡在对称位置诱发时，根据气泡形态总结出三种典型的塌陷特征，包括 V 形、T 形和银杏叶形。(2) 随着气泡初生位置在对称轴上的移动，开尔文脉冲强度呈现非单调趋势，其方向多次变化。脉冲强度和方向都受到粒子排列的显著影响。(3) 三个粒子中存在多个零脉冲点，零脉冲点的空间位置和数量都受粒子排列的影响。

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引用次数: 0

Study on the effect and mechanism of ultrasonic-assisted enzymolysis on antioxidant peptide activity in walnuts 超声波辅助酶解对核桃中抗氧化肽活性的影响和机理研究

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2024-11-17 DOI: 10.1016/j.ultsonch.2024.107159

Jiaying Huo , Zhaowei Cui , Ruru Zhang , Hui Ouyang , Xiaolong Liu , Ping Wang , Xiongwei Yu , Tingting Xie , Sihai Gao , Shugang Li

Walnut meal is a large quantity and high-quality resource with great exploitation value. Ultrasonic-assisted enzymolysis (UAE) was utilized in the preparation of peptides from walnut meal protein. Results indicated that by optimizing the UAE process with neutral protease, an ultrasound power of 180 W, a 4.3 h duration and an enzyme dosage of 10 KU/g, the walnut peptides exhibited the most potent antioxidant activity. In comparison to the control group, the WPI treated with ultrasound and neutral enzymes in combination (UNWPI) demonstrated a significant enhancement in their DPPH, ABTS, and ·OH scavenging capabilities, with increases of 234.23 %, 240.22 %, and 69.52 %, respectively. By analyzing the structure of walnut antioxidant peptides with or without ultrasound, it was observed that the underlying mechanism for the increased antioxidant activity was that UAE not only formed more small peptides, but also produced more peptides with hydrophobic amino acids at their terminal ends. Subsequently, six peptides were identified and screened from UNWPI, namely IFW, IIPF, IVAF, IIFY, ILAFF, and IFIP, which exhibited high antioxidant activity and could bind to Keap1 protein through hydrogen bonding, π-alkyl interactions, and π-π stacking interactions. The research results provided theoretical basis and technical support for the preparation of walnut antioxidant peptides and the high-value utilization of walnut meal.

核桃粉是一种量大质优、极具开发价值的资源。超声波辅助酶解（UAE）被用于从核桃粉蛋白中制备肽。结果表明，通过优化 UAE 工艺，使用中性蛋白酶、180 W 超声波功率、4.3 小时持续时间和 10 KU/g 酶用量，核桃肽表现出了最强的抗氧化活性。与对照组相比，经超声波和中性酶组合处理的核桃肽（UNWPI）的 DPPH、ABTS 和 -OH 清除能力显著增强，分别提高了 234.23 %、240.22 % 和 69.52 %。通过分析使用或不使用超声波的核桃抗氧化肽的结构，可以观察到抗氧化活性提高的根本机制是 UAE 不仅形成了更多的小肽，而且产生了更多末端带有疏水氨基酸的肽。随后，从 UNWPI 中鉴定并筛选出了六种肽，即 IFW、IIPF、IVAF、IIFY、ILAFF 和 IFIP，这六种肽具有较高的抗氧化活性，并能通过氢键、π-烷基相互作用和 π-π 堆积相互作用与 Keap1 蛋白结合。该研究成果为核桃抗氧化肽的制备和核桃粉的高值化利用提供了理论依据和技术支持。

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引用次数: 0