Ultrasonics Sonochemistry最新文献_第7页

Dual-stage ultrasound application for rice bran protein extraction: A novel method to improve yield, functional properties, and nutritional profile 双级超声在米糠蛋白提取中的应用：一种提高产量、功能特性和营养成分的新方法

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-14 DOI: 10.1016/j.ultsonch.2025.107681

Saydul Md Safwa, Nikitha Modupalli, Md Mahfuzur Rahman

While ultrasound-assisted protein extraction is well-studied, the application of ultrasound in both the extraction and precipitation stages has not been explored. This study explored the ultrasound (U)-assisted alkaline extraction (AE) at pH 9.5 and ultrasound-assisted isoelectric precipitation (UIP) at pH 3.5 on the rice bran protein yield, functionality, and nutritional quality. The UAE and conventional precipitation (UAE-IP) yielded the highest protein yield of 65.7 %, while conventional extraction yielded 38.9 %. FTIR showed that UAE-IP increased β-sheets by 96.8 % while AE-UIP decreased the α-helices by 42.4 %. Although UAE-IP showed no significant change in surface hydrophobicity, UAE-UIP decreased it by 34.7 % to the control. Consequently, functional properties such as solubility, emulsifying, and foaming capacities were significantly improved by 5 %, 0.05, and 3 % respectively. AE-UIP exhibited the highest solubility at pH 7, while UAE-IP showed better solubility at acidic and alkaline pHs. UAE-UIP demonstrated superior emulsion and foaming properties. Ultrasound application enhanced protein digestibility, with AE-UIP showing the highest digestibility of 96.91 %. However, dual-stage ultrasound reduced protein digestibility and subsequently affected the amino acid score (AAS). The amino acid profile varied with extraction methods, with AE-IP showing the highest levels of essential amino acids. AE-IP and UAE-IP showed no significant difference in PDCAAS (protein digestibility–corrected amino acid score) and AAS, whereas AE-UIP and UAE-UIP reduced them to 0.9 and 0.6, respectively. In summary, while ultrasound-assisted extraction enhances protein yield and certain properties, ultrasound-assisted precipitation shows great promise in further enhancing mass yield and functionality, which underscores the importance of a dual-stage ultrasound.

虽然超声辅助蛋白质提取已经得到了很好的研究，但超声在提取和沉淀阶段的应用还没有探索。本研究探讨了pH为9.5的超声(U)辅助碱性提取（AE）和pH为3.5的超声辅助等电沉淀法（UIP）对米糠蛋白产量、功能和营养品质的影响。UAE和常规沉淀法（UAE- ip）的蛋白质产量最高，为65.7% %，而常规提取法的蛋白质产量为38.9% %。FTIR结果表明，UAE-IP使α-螺旋增加96.8% %，AE-UIP使α-螺旋减少42.4 %。虽然UAE-IP对表面疏水性没有明显的影响，但与对照组相比，UAE-UIP使表面疏水性降低了34.7% %。因此，溶解度、乳化能力和起泡能力等功能性能分别显著提高了5 %、0.05和3 %。AE-UIP在pH值为7时的溶解度最高，而UAE-IP在酸性和碱性pH值下的溶解度较好。UAE-UIP具有优异的乳化和发泡性能。超声处理提高了蛋白质的消化率，AE-UIP的消化率最高，为96.91 %。然而，双级超声降低了蛋白质消化率，随后影响了氨基酸评分（AAS）。氨基酸分布随提取方法的不同而变化，AE-IP中必需氨基酸含量最高。AE-IP和UAE-IP在蛋白质消化率校正氨基酸评分（PDCAAS）和AAS方面无显著差异，而AE-UIP和UAE-UIP分别使PDCAAS和AAS降低至0.9和0.6。综上所述，超声辅助提取提高了蛋白质收率和某些特性，超声辅助沉淀在进一步提高质量收率和功能方面显示出巨大的前景，这强调了双级超声的重要性。

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引用次数: 0

Metabolic nanoblockers for synergistic cancer treatment through glutaminase inhibition and sonodynamic therapy 代谢纳米阻滞剂通过谷氨酰胺酶抑制和声动力疗法协同治疗癌症

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-14 DOI: 10.1016/j.ultsonch.2025.107680

Wen Zhao , Jintao Du , Yunxuan Song , Zilin Ma , Songyan Li , Yuhua Wei , Guiqiang Zhang

The immunotherapeutic potential of sonodynamic therapy (SDT) is hindered by tumor defense mechanisms driven by glutamine metabolism, including glutathione (GSH)-dependent redox homeostasis and an immunosuppressive tumor microenvironment (TME). To address these challenges, we herein developed metabolic nanoblockers (CBE@AM NPs) by encapsulating a glutamine metabolism inhibitor CB839 and a sonosensitizer chlorin e6 (Ce6) into melanin-inspired nanoparticles. The resulting nanoblockers triggered robust reactive oxygen species (ROS) production upon ultrasound irradiation, thereby destroying tumor cells and inducing immunogenic cell death (ICD). Concurrently, they inhibited the glutamine metabolism in the tumor, disrupting redox homeostasis and remodeling the immunosuppressive TME, thereby amplifying both SDT-generated oxidative stress and ICD-induced antitumor immunity. CBE@AM NPs demonstrated a potent tumor-inhibitory effect in tumor-bearing mice, highlighting their potential for immunometabolic reprogramming to enhance the therapeutic efficacy of SDT.

由谷氨酰胺代谢驱动的肿瘤防御机制，包括谷胱甘肽（GSH）依赖的氧化还原稳态和免疫抑制肿瘤微环境（TME），阻碍了声动力疗法（SDT）的免疫治疗潜力。为了解决这些挑战，我们在此通过将谷氨酰胺代谢抑制剂CB839和声敏剂氯e6 （Ce6）封装到黑色素激发的纳米颗粒中，开发了代谢纳米阻断剂（CBE@AM NPs）。由此产生的纳米阻滞剂在超声照射下触发强大的活性氧（ROS）产生，从而破坏肿瘤细胞并诱导免疫原性细胞死亡（ICD）。同时，它们抑制肿瘤中的谷氨酰胺代谢，破坏氧化还原稳态，重塑免疫抑制TME，从而放大sdt产生的氧化应激和icd诱导的抗肿瘤免疫。CBE@AM NPs在荷瘤小鼠中显示出强大的肿瘤抑制作用，突出了它们通过免疫代谢重编程增强SDT治疗效果的潜力。

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引用次数: 0

Eco-friendly production of AgNPs by ultrasound-intensified continuous method, and process evaluation via life cycle assessment and machine learning 超声波强化连续法生产AgNPs，并通过生命周期评估和机器学习进行工艺评估

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-14 DOI: 10.1016/j.ultsonch.2025.107682

Juncheng Hu , Wenyu Nie , Suxu Zhao , Yong Liu , Hengyi Zhu , Shijie Tu , Jiawei Zhang , Kris Y. Yang , Ning Xue , Justin Z. Lian , Bin Dong , Stefano Cucurachi , Yuan Gao

Owing to their excellent biocompatibility and antibacterial properties, the global annual production of silver nanoparticles (AgNPs) is estimated at 400–800 tons. Therefore, developing a green and safe approach for AgNPs synthesis is urgently required. However, the conventional AgNPs manufacturing methods required toxic reductant and higher energy consumption, which makes the reaction system harmful to humans and environment. In this study, an eco-friendly ultrasound-intensified continuous system employing minimal toxic reagents was developed. The AgNPs formation process was intensified by cavitation instead of using toxic and strong reductants. Several analytical methods were conducted to systematically investigate the mechanism and performance of as-synthesized AgNPs, such as particle size distribution, zeta potential, UV–visible absorption, morphology, yield calculation, as well as in vitro photothermal, and antibacterial. The results clearly indicated that the physicochemical properties of AgNPs could be tuned by adjusting process parameters, including temperature, feeding rate, and ultrasonic power. Specifically, the AgNPs prepared at 90 ℃, 600 W and 50 µL/min exhibited smaller size (2–5 nm) with monodisperse, spherical shape, higher yield, enhanced photothermal, and antibacterial activities. Furthermore, life-cycle assessment (LCA) and machine learning (ML) were employed to evaluate the process sustainability and identify the key influencing parameters. Comprehensive consideration of safety, experimental results, and LCA outcomes demonstrated that the ultrasound-intensified continuous method proposed in this study is superior to the traditional NaBH₄-based batch synthesis at the laboratory scale.

由于其优异的生物相容性和抗菌性能，全球纳米银（AgNPs）的年产量估计在400-800吨之间。因此，开发一种绿色、安全的AgNPs合成方法是迫切需要的。然而，传统的AgNPs制造方法需要有毒的还原剂和较高的能量消耗，这使得反应体系对人类和环境有害。在本研究中，开发了一种使用最小毒性试剂的环保超声强化连续系统。用空化代替毒性强的还原剂，强化了AgNPs的形成过程。通过粒度分布、zeta电位、紫外可见吸收、形貌、产率计算、体外光热性能、抗菌性能等分析方法，系统考察了AgNPs的机理和性能。结果表明，AgNPs的物理化学性质可以通过调节工艺参数，包括温度、加料速率和超声波功率来调节。其中，在90 ℃、600 W和50 µL/min条件下制备的AgNPs具有尺寸较小（2-5 nm）、单分散、球形、产率高、光热和抗菌活性增强等特点。此外，采用生命周期评估（LCA）和机器学习（ML）来评估过程可持续性并确定关键影响参数。综合考虑安全性、实验结果和LCA结果，本研究提出的超声强化连续合成方法在实验室规模上优于传统的基于nabh4的间歇合成方法。

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引用次数: 0

Synergistic degradation of levofloxacin (LEV) by Cu2+-activated peroxymonosulfate (PMS) under hydrodynamic cavitation (HC): Efficiency and mechanistic insights 水动力空化（HC）条件下Cu2+活化过氧单硫酸根（PMS）协同降解左氧氟沙星（LEV）的效率和机理

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-13 DOI: 10.1016/j.ultsonch.2025.107679

Zheng Li , Weibin You , Sivakumar Manickam , Haiyan Bie , Wenlong Wang , Xun Sun

To effectively eliminate excess antibiotics from aqueous environments and to mitigate the dissemination of antibiotic resistance genes (ARGs), this study proposes a novel degradation system that activates peroxymonosulfate (PMS) through a synergistic combination of hydrodynamic cavitation (HC) and divalent copper ions (Cu²⁺). Levofloxacin (LEV) is employed as the representative target contaminant to evaluate the system’s performance. HC has emerged as a promising technique for pollutant removal. In this study, the localized high-temperature and high-pressure conditions generated by HC not only partially activated PMS but also facilitated its interaction with Cu²⁺ ions, leading to a pronounced synergistic enhancement in sulfate radical (SO₄⁻) generation and efficient pollutant degradation. Under optimized HC/Cu²⁺/PMS conditions (Cu²⁺ = 5 mM, PMS = 2.5 mM, inlet pressure = 0.15 MPa, pH = 10), complete removal of LEV (30 mg/L) was achieved within 50 min. This study elucidates the degradation mechanisms and pathways of LEV within the coupled HC/Cu²⁺/PMS system and evaluates the ecological safety of its degradation intermediates using the U.S. EPA’s T.E.S.T. (Toxicity Estimation Software Tool). Furthermore, the system’s applicability was validated through degradation experiments involving a range of representative pollutants, demonstrating its broad-spectrum effectiveness. Crucially, the HC/Cu²⁺/PMS system demonstrated a superior cavitation yield (2.78 × 10⁻⁵ mg/J) and a low electrical energy per order (EE/O) of 229.48 kWh/m³, highlighting its high energy efficiency and practical potential for sustainable wastewater treatment. The experimental results emphasize the system’s strong potential for the effective removal of organic pollutants from water, offering a novel and sustainable approach for advanced water treatment.

为了有效地消除水环境中过量的抗生素并减轻抗生素耐药基因（ARGs）的传播，本研究提出了一种新的降解系统，该系统通过水动力空化（HC）和二价铜离子（Cu2+）的协同组合激活过氧单硫酸根（PMS）。以左氧氟沙星（LEV）为代表性目标污染物评价系统的性能。HC已成为一种很有前途的污染物去除技术。在本研究中，HC产生的局部高温高压条件不仅部分激活了PMS，而且促进了PMS与Cu2+离子的相互作用，从而显著增强了硫酸盐自由基（SO4−）的生成和有效的污染物降解。HC / Cu2 + / PMS优化条件下(Cu2 + = 5 mM, PMS = 2.5 mM,进气压力 = 0.15 MPa, pH值 = 10),完整切除列弗(30 mg / L)在50 分钟之内就完成了。本研究阐明了HC/Cu2+/PMS耦合体系中LEV的降解机制和途径，并利用美国环保局的毒性评估软件工具（T.E.S.T.）对其降解中间体的生态安全性进行了评价。此外，通过涉及一系列代表性污染物的降解实验验证了该系统的适用性，证明了其广谱有效性。最重要的是，HC/Cu2+/PMS系统表现出优异的空化产率（2.78 × 10−5 mg/J）和低的每单次电能（EE/O）为229.48 kWh/m3，突出了其高能效和可持续废水处理的实际潜力。实验结果强调了该系统在有效去除水中有机污染物方面的强大潜力，为高级水处理提供了一种新颖且可持续的方法。

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引用次数: 0

Unveiling the pathways of flavor formation in marinated lamb driven by ultrasound-assisted vacuum tumbling: An integrated multi-omics approach 揭示超声辅助真空翻滚驱动腌羊肉风味形成的途径：一种集成的多组学方法

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-12 DOI: 10.1016/j.ultsonch.2025.107677

Chenchen Xu , Shouwei Wang , Yan Zhao , Jiapeng Li , Shunliang Zhang , Jing Bai , Yuxuan Shi , Xianqi Yao , Anyu Gao , Bing Zhao

The development of desirable flavor in processed lamb remains a significant challenge for the meat industry. This study aimed to investigate the effects of ultrasound-assisted vacuum tumbling (UVT) on the flavor characteristics of lamb using a combined analytical approach of flavoromics, lipidomics, and metabolomics. The results indicated that UVT significantly expedited the degradation and subsequent oxidation of crucial flavor precursors, including phospholipid metabolites (phosphatidylethanolamine, phosphatidylserine, cytidine diphosphate-diglyceride, phosphatidylcholine, and lysophosphatidylcholine), glyceride metabolites (triacylglycerols), nucleotide metabolites (inosine monophosphate and guanosine monophosphate), and amino acid metabolites (L-glutamine). This acceleration was associated with the formation of desirable flavor compounds in the marinated lamb, including Hexanal, 1-Octen-3-ol, Methional, 2-Acetylfuran, and various esters. In conclusion, this study demonstrates that UVT is a powerful strategy for intensifying and controlling the flavor profile of meat products, providing a robust theoretical foundation for the precise application of ultrasound technology in the modern meat industry.

在加工羊肉中开发理想的风味仍然是肉类工业面临的重大挑战。本研究旨在利用风味组学、脂质组学和代谢组学相结合的分析方法，研究超声辅助真空翻滚（UVT）对羊肉风味特性的影响。结果表明，UVT显著加速了关键风味前体的降解和随后的氧化，包括磷脂代谢物（磷脂酰乙醇胺、磷脂酰丝氨酸、胞苷二磷酸二甘酯、磷脂酰胆碱和溶血磷脂酰胆碱）、甘油代谢物（三酰基甘油）、核苷酸代谢物（单磷酸肌苷和单磷酸鸟苷）和氨基酸代谢物（l -谷氨酰胺）。这种加速与腌羊肉中理想风味化合物的形成有关，包括己醛、1-辛-3-醇、甲基、2-乙酰呋喃和各种酯。综上所述，该研究表明UVT是强化和控制肉制品风味特征的有力策略，为超声技术在现代肉类工业中的精确应用提供了坚实的理论基础。

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引用次数: 0

Ultrasonic-Assisted extraction of luteolin from peanut shells using supramolecular solvents and its molecular mechanism 超分子溶剂超声辅助提取花生壳木犀草素及其分子机理研究

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-12 DOI: 10.1016/j.ultsonch.2025.107678

Yuhan Fang , Ping Zhang , Shuang Wang , Lulu Li , Zunlai Sheng

This study innovatively employs ultrasound-assisted extraction (UAE) in conjunction with supramolecular solvents (SUPRAS) for the extraction of luteolin from peanut shells, while also investigating the potential mechanism underlying this extraction process. Initially, the formation mechanism of SUPRAS was characterized using Fourier-transform infrared spectroscopy (FTIR). Based on the results of single-factor experiments, the extraction process was optimized using response surface methodology (RSM), resulting in the determination of optimal conditions: an ultrasound temperature of 67 °C, a SUPRAS/equilibrium solution (EqS) volume ratio of 5:1, and a solid-to-liquid ratio of 1:36 g/mL. Under these conditions, the maximum luteolin yield reached 1.645 mg/g. Furthermore, through independent gradient model (IGMH) calculations, molecular orbital theory, interaction energy analysis, and molecular dynamics simulations, it was revealed that the predominant interaction force between the supramolecular solvent and the target compound is hydrogen bonding. Notably, this study also compares the SUPRAS-UAE extraction method with the traditional extraction in terms of efficiency, economic costs, and environmental impact. The results indicate that the SUPRAS-UAE technique demonstrates significant advantages in terms of its green and high-efficiency characteristics. It provides a new sustainable strategy for the efficient recovery of natural active ingredients in chemical engineering and related fields.

本研究创新性地采用超声辅助萃取（UAE）联合超分子溶剂（SUPRAS）从花生壳中提取木犀草素，并探讨了这一提取过程的潜在机制。首先，利用傅里叶变换红外光谱（FTIR）表征了SUPRAS的形成机理。在单因素实验的基础上，采用响应面法（RSM）对提取工艺进行优化，确定最佳提取条件为：超声温度67℃，SUPRAS/平衡溶液（EqS）体积比为5:1，料液比为1:36 g/mL。在此条件下，木犀草素产量最高可达1.645 mg/g。此外，通过独立梯度模型（IGMH）计算、分子轨道理论、相互作用能分析和分子动力学模拟，揭示了超分子溶剂与目标化合物之间的主要相互作用力是氢键。值得注意的是，本研究还将supra - uae提取方法与传统提取方法在效率、经济成本和环境影响方面进行了比较。结果表明，supra - uae技术在绿色和高效方面具有显著的优势。为化工及相关领域高效回收天然活性成分提供了新的可持续发展策略。

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引用次数: 0

Enhancing Nucleation using a Vortex-based Hydrodynamic Cavitation Device: Application to Antisolvent Crystallization of Paracetamol – Methanol – Water System 涡流型水动力空化装置增强成核：在扑热息痛-甲醇-水体系反溶剂结晶中的应用

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-11 DOI: 10.1016/j.ultsonch.2025.107668

Vidit Tiwari , Subhrajit Swain , Vivek V. Ranade

Nucleation is the onset of the crystallization process, and enhancing its rate is fundamental to intensifying crystallization, be it in a batch or continuous mode of operation. The present work investigates, for the first time, the influence of hydrodynamic cavitation (HC) on enhancing the nucleation rate of paracetamol crystallization in a methanol–water system. Initially, the experiments were performed in a batch mode to determine the induction time using a vortex-based HC device (VD). The experimentally determined induction time was found to decrease with supersaturation. The use of HC was found to enhance nucleation and significantly reduce induction time. An appropriate correlation to estimate induction time is developed. Based on these findings, the VD-based continuous nucleator using a loop configuration was developed to intensify the performance of a continuous oscillatory baffle crystallizer (COBC). The VD-based nucleator improved the yield and productivity of the COBC, significantly reducing the risk of encrustation and clogging in the bends of the COBC. The approach and results presented in the current study will provide a sound basis for harnessing VD-based hydrodynamic cavitation for enhancing nucleation and intensifying antisolvent crystallization.

成核是结晶过程的开始，提高成核速率是强化结晶的基础，无论是在间歇还是连续操作模式下。本文首次研究了在甲醇-水体系中流体动力空化（HC）对提高对乙酰氨基酚结晶成核速率的影响。首先，使用基于涡的HC装置（VD）以批处理模式进行实验，以确定诱导时间。实验确定的诱导时间随着过饱和度的增加而减少。发现HC的使用可以增强成核并显著缩短诱导时间。建立了一个适当的相关性来估计感应时间。基于这些发现，开发了基于dvd的连续成核器，采用环路结构来增强连续振荡挡板结晶器（COBC）的性能。基于dvd的成核器提高了COBC的产量和生产率，显著降低了COBC弯道结皮和堵塞的风险。本研究的方法和结果将为利用基于真空的流体动力空化来增强成核和增强抗溶剂结晶提供良好的基础。

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引用次数: 0

Ultrasonically synthesized Cu/Ag/Zn nanocomposite coatings for antibacterial and antibiofilm applications in urological devices 超声合成Cu/Ag/Zn纳米复合涂层用于泌尿外科设备的抗菌和抗菌膜

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-11 DOI: 10.1016/j.ultsonch.2025.107674

Houhong Wang , Chun Luo , Wenli Chen , Weilin Wang , Yongyun Chen , Kelei Shang , Yan Peng , Zhongmin Li

Catheter-associated urinary tract infections (CAUTIs) pose a persistent clinical challenge due to bacterial colonization and biofilm formation on indwelling urological devices. This study investigates the efficacy of Cu/Ag/Zn trimetallic nanocomposites (TMNCs) as antimicrobial coatings for urinary catheters, synthesized via a green, ultrasonic–autoclave-assisted method. The nanocomposites were thoroughly characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), Brunauer–Emmett–Teller (BET) surface area analysis, and field emission scanning electron microscopy (FESEM) to confirm their mesoporous, crystalline, uniformly distributed, and thermally stable nanoparticles. An optimized ultrasonic embedding technique was employed to uniformly coat TMNCs onto commercial urinary catheters, ensuring strong adhesion and consistent coverage. Antibacterial assays demonstrated concentration-dependent inhibition zones, reaching up to 21.5 mm for Escherichia coli and 24.0 mm for Staphylococcus aureus. Minimum inhibitory concentration (MIC) tests confirmed potent bactericidal activity, with MIC values of 32 µg/mL for E. coli and 64 µg/mL for S. aureus. Anti-biofilm assessments revealed that TMNCs matched or outperformed the efficacy of vancomycin, achieving up to 86.12 % inhibition for S. aureus and 73.59 % for E. coli at 2 × MIC. Cytotoxicity testing using U87 glioblastoma cells indicated good biocompatibility, with over 79 % cell viability at 0.1 mg/mL, followed by a dose-dependent decline at higher concentrations. Long-term stability studies conducted over 60 days under varied storage conditions confirmed the photothermal and colloidal stability of the TMNCs. In conclusion, Cu/Ag/Zn TMNC-coated urinary catheters demonstrated excellent antibacterial and anti-biofilm properties, biocompatibility, and long-term stability. These multi-functional coatings present a promising strategy for reducing CAUTI incidence and improving patient safety and clinical outcomes in urological applications.

导尿管相关性尿路感染（CAUTIs）由于细菌定植和生物膜的形成，对留置泌尿系统设备构成了持续的临床挑战。本研究研究了Cu/Ag/Zn三金属纳米复合材料（TMNCs）作为导尿管抗菌涂层的效果，该材料是通过绿色超声-高压灭菌辅助方法合成的。利用x射线衍射（XRD）、x射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）、拉曼光谱（Raman）、热重分析（TGA）、布鲁诺尔-埃米特-泰勒（BET）表面积分析和场发射扫描电镜（FESEM）对纳米复合材料进行了全面表征，证实了纳米颗粒具有介孔、结晶性、均匀分布和热稳定性。采用一种优化的超声包埋技术，将tmnc均匀地包埋在商用导尿管上，保证了其强附着力和一致的覆盖。抑菌试验显示出浓度依赖的抑制区，大肠杆菌和金黄色葡萄球菌的抑制区分别达到21.5 mm和24.0 mm。最低抑菌浓度（MIC）试验证实了有效的杀菌活性，大肠杆菌的MIC值为32µg/mL，金黄色葡萄球菌的MIC值为64µg/mL。抗生物膜评价显示，在2 × MIC下，TMNCs对金黄色葡萄球菌的抑制率高达86.12%，对大肠杆菌的抑制率为73.59%，与万古霉素的效果相当或优于万古霉素。使用U87胶质母细胞瘤细胞进行的细胞毒性测试显示出良好的生物相容性，在0.1 mg/mL浓度下细胞存活率超过79%，随后在更高浓度下呈剂量依赖性下降。在不同的储存条件下进行了超过60天的长期稳定性研究，证实了tmnc的光热稳定性和胶体稳定性。综上所述，Cu/Ag/Zn tmnc涂层导尿管具有良好的抗菌和抗生物膜性能、生物相容性和长期稳定性。这些多功能涂层提供了一个很有前途的策略，以减少CAUTI的发病率，提高患者的安全性和临床结果在泌尿外科应用。

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引用次数: 0

Unveiling the mechanism of ultrasound-assisted phenolic extraction from Psidium cattleianum leaves: Kinetic, mass transfer, and thermodynamic insights 揭示超声波辅助从牛菖蒲叶中提取酚类物质的机理：动力学、传质和热力学见解

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-11 DOI: 10.1016/j.ultsonch.2025.107675

Hoang Duy Huynh , Parushi Nargotra , Hui-Min David Wang , Yung-Hsiang Tsai , Chien-Chih Chiu , Chwen-Jen Shieh , Yung-Chuan Liu , Chia-Hung Kuo

Strawberry guava leaves (Psidium cattleianum) are an attractive phenolic-rich byproduct for food and pharmaceutical production, with potential benefits for human health due to their exceptional antioxidant activity among plant-based resources. However, there are no systematic studies on sonochemical phenolic extraction from this material. Therefore, the goal of this study was to unveil the mechanism of ultrasound-assisted extraction (UAE) for phenolic recovery from Psidium cattleianum leaves. Pseudo-second-order kinetic modeling, Biot number, and thermodynamic analysis were introduced to describe the extraction mechanisms and then kinetic parameters such as saturation concentration (C_s), extraction rate (k), diffusion coefficient (D_eff), and mass transfer coefficient (K_T) compared with conventional shaking extraction (CSE) to establish the process intensification benefits. The result of pseudo-second-order kinetic analysis revealed a biphasic extraction mechanism consisting of initial rapid release, followed by diffusion-controlled mass transfer, with internal mass transfer identified as the rate-limiting step (Bi > 50). The UAE process was spontaneous (ΔG^o < 0), endothermic (ΔH^o = 3.8151 kJ/mol), and entropy-driven (ΔS^o > 0). The presence of ultrasound technology enabled UAE to demonstrate superior performance compared to CSE, which significantly increased C_s, k, D_e and K_T. Taken together, the integration of kinetic and thermodynamic analyses offers a robust framework for understanding sonochemical processes and fundamental insights into the nature of UAE from Psidium cattleianum leaves, thereby overcoming scale-up challenges and facilitating industrial applications.

草莓番石榴叶（Psidium cattleanum）是一种有吸引力的富含酚的食品和制药副产品，由于其在植物性资源中具有出色的抗氧化活性，对人类健康具有潜在的益处。然而，目前还没有系统的研究从这种材料中提取声化学酚。因此，本研究的目的是揭示超声辅助提取（UAE）从牛菖蒲叶中提取酚类物质的机理。引入准二阶动力学模型、Biot数和热力学分析来描述萃取机理，并通过饱和浓度（Cs）、萃取速率(k)、扩散系数（Deff）和传质系数（KT）等动力学参数与常规摇提（CSE）进行比较，确定工艺强化效益。准二级动力学分析结果显示，萃取过程为初始快速释放，然后是扩散控制的传质，其中内部传质为限速步骤（Bi > 50）。UAE过程为自发反应（ΔGo < 0）、吸热反应（ΔHo = 3.8151 kJ/mol）和熵驱动反应（ΔSo > 0）。与CSE相比，超声技术的存在使UAE表现出优越的性能，显著提高了Cs， k， De和KT。综上所述，动力学和热力学分析的整合为理解声化学过程和从牛菖蒲叶子中获得阿联酋性质的基本见解提供了一个强大的框架，从而克服了扩大规模的挑战，促进了工业应用。

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引用次数: 0

Effect of ultrasound treatment on kidney bean protein isolate-oat β-glucan glycation conjugates: The structure, digestibility and emulsifying properties 超声处理对芸豆分离蛋白-燕麦β-葡聚糖糖基化偶联物的影响：结构、消化率和乳化性能

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-11-11 DOI: 10.1016/j.ultsonch.2025.107676

Zhigang Sun , Chaojiang Dong , Yue Gu , Yiying Jin , Fangxiao Lou , Tianfu Cheng , Lingling Zhang , Zengwang Guo

Conjugates of kidney bean protein isolate (KBPI) and oat β-glucan (OG) were successfully prepared via ultrasonic-assisted Maillard reaction (wet heating). The effects of ultrasonic power density (9.62–29.37 W/cm²) on its structural characteristics, in vitro digestive behavior, and emulsifying properties were investigated. The results demonstrated that ultrasonic treatment significantly enhanced the covalent conjugation between KBPI and OG. Specifically, the ultrasonically modified conjugate (UHKBPI-OG3) prepared at 22.42 W/cm² exhibited higher grafting degree of 23.53 %. Appropriate ultrasonic treatment reduced the average particle size and polydispersity index (PDI) of conjugates, as well as increasing the absolute value of their ζ-potential. Circular dichroism (CD) analysis revealed that ultrasound-assisted wet heating induced conformational changes in KBPI, showing a significant reduction in α-helix content accompanied by an increase in random coil structure. This structural transition suggests enhanced molecular flexibility, which is favorable for achieving higher glycosylation efficiency. Ultrasonic treatment enhanced the emulsifying properties of UHKBPI-OG3, increasing emulsifying activity index (EAI) and emulsion stability index (ESI) by 20.75 % and 15.65 %, respectively, compared to wet heating. Concurrent reductions in interfacial tension, apparent viscosity, and droplet size further contributed to improved emulsion stability. Furthermore, UHKBPI-OG3 conjugate (24.22 W/cm²) exhibited the lowest protein digestibility. These findings demonstrate that optimized ultrasonic power density enhances KBPI-OG covalent conjugation. The improved functional properties of conjugates, achieved through modulated protein-polysaccharide interactions, provide theoretical support for developing novel emulsion stabilizers.

采用超声辅助美拉德反应（湿加热）法制备了芸豆分离蛋白（KBPI）与燕麦β-葡聚糖（OG）的偶联物。研究了超声功率密度（9.62 ~ 29.37 W/cm2）对其结构特性、体外消化行为和乳化性能的影响。结果表明，超声处理显著增强了KBPI与OG之间的共价共轭作用。其中，在22.42 W/cm2下制备的超声修饰共轭物（UHKBPI-OG3）接枝度较高，达到23.53%。适当的超声处理降低了共轭物的平均粒径和多分散性指数（PDI），并增加了共轭物的ζ势绝对值。圆二色性（CD）分析表明，超声辅助湿加热诱导KBPI构象发生变化，α-螺旋含量显著降低，随机螺旋结构增加。这种结构转变表明分子柔韧性增强，有利于实现更高的糖基化效率。超声处理提高了UHKBPI-OG3的乳化性能，乳化活性指数（EAI）和乳液稳定性指数（ESI）分别比湿法加热提高了20.75%和15.65%。界面张力、表观粘度和液滴尺寸的同时降低进一步提高了乳液的稳定性。此外，UHKBPI-OG3偶联物（24.22 W/cm2）的蛋白质消化率最低。结果表明，优化后的超声功率密度可以增强KBPI-OG的共价共轭作用。通过调节蛋白质-多糖的相互作用，改善了缀合物的功能特性，为开发新型乳液稳定剂提供了理论支持。

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引用次数: 0