Biology-Basel最新文献

Mining activities in arid regions of China have led to severe environmental degradation, including soil erosion, vegetation loss, and contamination of soil and water resources. These impacts are particularly pronounced in abandoned mining areas, where the cessation of mining operations has left vast landscapes unrehabilitated. In response, the Chinese government has implemented a series of legal and regulatory frameworks, such as the "Mine Environmental Protection and Restoration Program", aimed at promoting ecological restoration in these areas. However, the unique environmental conditions of arid regions, including water scarcity, extreme temperatures, and poor soil quality, present significant challenges to restoration efforts. This review provides a comprehensive analysis of the ecological restoration of abandoned mining areas in China's arid regions, focusing on the legal framework, restoration techniques, and evaluation systems. The restoration methods, their initiation timelines, monitoring systems, and the cost-benefit aspects of various strategies are critically reviewed alongside case studies from regions such as the Mu Us Desert and Qaidam Basin. Key strategies like phytoremediation, soil rehabilitation, and water resource management are assessed for their effectiveness, while challenges in enforcement, socioeconomic integration, and community engagement are discussed. This review concludes that while significant progress has been made, further improvements in restoration practices and evaluation systems are essential for long-term sustainability. Integration of socioeconomic indicators, community involvement, and advanced monitoring technologies are necessary for successful outcomes.

The intraspecies and interspecies Comparative Genomic Hybridization (CGH) between the closely related Cebidae species, capuchin monkeys (Cebus capucinus, Sapajus apella), and the tamarins (Saguinus mystax, Leontocebus fuscicollis) was performed to analyze their genomes. In particular, this approach determines balanced and unbalanced repetitive DNA sequence distribution and reveals dynamics during evolution. Capuchin monkeys are considered the most ancestral group with conserved syntenies compared to the hypothetical ancestral New World monkeys' karyotype. Also, more derived karyotypes of phylogenetically distant species from the Saguinus and Leontocebus genera are analyzed here. The distribution of repetitive sequences has been traditionally studied through classical staining methods of cytogenetics. It has been hypothesized that repeats are species-specific and their conservation across closely related species are also common; their role in the genome has been extensively studied even though its role in speciation is not well studied and understood. The CGH shows bright signals with balanced and imbalanced DNA involving different genome regions: such as predominantly repetitive DNA at centromeric positions, and interstitial distribution with extended blocks. Cross-species CGH demonstrated the origin of some heterochromatic regions and identified apomorphic heterochromatin expansion events. The uncovered distribution of repetitive sequences is analyzed from an evolutionary perspective to elucidate the genomic dynamics of the repetitive sequences at the level of chromosomal organization.

Gerbera (Gerbera hybrida) is a popular cut flower on the market, so extending its vase life (VL) is an important goal in the horticultural industry. The aim of this study was to improve the freshness of gerbera cut flowers through the optimal solution (OS) and to analyze its preservation mechanism. We used chitosan (COS), calcium chloride (CaCl₂), and citric acid (CA) as the main ingredients of the vase solution and determined the OS ratio of 104 mg/L of COS, 92 mg/L of CA, and 93 mg/L of CaCl₂ using the Box-Behnken design-response surface method (BBD-RSM). Gerbera preservation results showed that the VL of the OS was 14.5 days, which was significantly longer than that of flowers maintained in the Basic Vase Solution (BVS) and the Commercial Formulation (CF) and was highly consistent with the theoretical VL of 14.57 d. Transcriptome analysis indicated that the OS might extend VL by regulating phytohormone signaling pathways, such as cytokinin and salicylic acid signaling. The qRT-PCR analysis of key candidate genes supported these findings, with significant upregulation observed in genes related to cytokinin synthesis (e.g., GhIPT1 and GhIPT9), salicylic acid signaling related to pathogen defense (e.g., GhTGA1, GhTGA4, GhNPR1, and GhRBOHA), and plant wax synthesis and stress response (e.g., GhKCS5, GhCUT1, and GhKCS6). Further, transcriptome GO-enrichment and physiological analysis showed that the OS might extend VL of Gerbera cut flowers by scavenging reactive oxygen species, including by activating the expression of genes related to oxidoreductase activity and the activities of antioxidant-system-related enzymes catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and superoxide dismutase (SOD), while decreasing the malondialdehyde (MDA) content. These results provide valuable insights into the mechanisms underlying the extended VL of gerbera cut flowers and offer a foundation for developing more effective preservation techniques.

The abiotic stresses to which plants are exposed, especially in times of climate change, can result in the disruption of natural plant physiological processes. Sudden atmospheric phenomena may increase the risk of failure in protecting rare and extinction-threatened plant species by translocation. This study aimed to determine the effect of extreme ambient temperatures on the condition and physiological response of Salix lapponum plantlets used for their reintroduction into the natural habitat. Salix lapponum plants obtained by micropropagation methods at different stages of growth under laboratory conditions were subjected to a biological experiment. Plants were exposed for 12 h to temperature extremes (0 °C and 30 °C), after which the values of selected markers of the biochemical response were determined, such as photosynthetic pigments and anthocyanin content, guaiacol peroxidase and catalase activity, the presence of ROS and the RWC value. The study showed that plants at early growth stages were sensitive to low-temperature stress. In contrast, older ones showed a stronger response to high temperature, marked by an increased anthocyanin content and guaiacol peroxidase activity. It was also found that a short exposure to temperature extremes did not change the photosynthetic pigment content or catalase activity. The results of the study may be an important indication for the optimization of plant acclimatization methods in the process of their active protection by species translocation.

Antimicrobial resistance (AMR) poses a critical global health threat, driving the search for alternative treatments to conventional antibiotics. In this study, the antibacterial properties of honeybee venom (BV) and fungal Monascus purpureus red dye (RD) were evaluated against three multidrug-resistant bacterial pathogens. Extracts of BV and RD exhibited dose-dependent antibacterial activity against the three tested bacteria, with their strongest effectiveness against S. aureus (minimum inhibitory concentrations [MIC] = 3.18 and 6.315 μg·mL^-1, respectively). Although the three bacterial strains were resistant to the antibiotic ampicillin-sulbactam (10/10 µg), both extracts exhibited superior antibacterial activity against the three bacterial strains compared to five standard antibiotics, especially RD extract, which produced the largest inhibition zone (20 ± 0.20 mm) against S. aureus. The larger inhibition zones against S. aureus suggest its high sensitivity, whereas E. coli and E. faecalis exhibited smaller inhibition zones, indicating less sensitivity to BV and RD extracts. Differences in the inhibition zones suggest the variations in antimicrobial activity between the two extracts and their strain-specific effectiveness. Scanning electron microscopy (SEM) revealed that BV and RD extracts disrupted the bacterial plasma membrane, suggesting that the bioactive compounds penetrate the bacterial cell wall and alter its integrity. Furthermore, GC-MS-based analysis revealed that the chemical composition of BV and RD extracts exhibited highly diverse structures, including complex polycyclic systems, porphyrins, steroids, and esters. For instance, 42 metabolites were identified in the BV extract, which mainly were organic and metal-organic compounds; however, only 23 molecules were identified in RD extract, which mainly were fatty acids and their derivatives. The diversity in the chemical compositions of both extracts highlights their potential applications in pharmaceuticals, materials, and biochemistry fields. Collectively, these findings indicate that honeybee venom and the red dye from M. purpureus have promising antibacterial properties and warrant further investigation as potential alternatives to conventional antibiotics. Further multi-ligand docking-based virtual screening studies are required to identify the most promising detected metabolite(s) within both BV and RD extracts.

Plant lectin receptor-like kinases (LecRLKs) are plant membrane protein receptor kinases. Lectin-like receptor kinases play a crucial role in regulating plant growth, development, and responses to environmental stimuli. It can rapidly respond to both biotic and abiotic stresses while mediating mechanisms of plant immune responses. This study represents the first identification of the LecRLK family genes in maize. It analyzes the gene structure, chromosomal locations, phylogenetic classification, promoter homoeotropic elements, and expression patterns under both biotic and abiotic stresses. The results indicate that these genes possess kinase and transmembrane domains, along with specific L-type or G-type extracellular domains. Most ZmLecRLK gene promoters contain cis-acting elements that are responsive to known hormones and stressors. Furthermore, these genes have been identified as being sensitive to both biotic and abiotic stresses. This discovery establishes a significant theoretical foundation for the selection of corn varieties in adverse environments. Additionally, it provides a basis for further in-depth exploration of the molecular regulatory mechanisms of LecRLK family genes.

Native to tropical America, the charru mussel, Mytella strigata, has been spreading rapidly in the West Pacific Ocean, including the South China Sea. In order to study the adaptive evolution of M. strigata and examine the present status of invasion in China, the mitochondrial nad2 gene fragment was employed to analyze the genetic variations of seven populations sampled in both spring and autumn 2023. Results showed that all the populations had high haplotype diversity (>0.5) and low nucleotide diversity (<0.005), suggesting the ongoing rapid expansion following a genetic bottleneck. The Zhanjiang population had the highest genetic diversity in spring with 22 haplotypes, 37 polymorphic sites, and haplotype diversity, nucleotide diversity, and the average number of nucleotide differences being 0.911, 0.00623, and 4.341, respectively. However, in autumn, the Shanwei population had the most haplotypes (11) and polymorphic sites (19), with the highest haplotype diversity value of 0.891, while the Qunjian population had the highest nucleotide diversity (0.00392) and average number of nucleotide differences (2.809). Combining geographic populations by seasons confirmed lower genetic diversity in autumn compared to spring, evidenced by fewer haplotypes and polymorphic sites, reduced haplotype diversity and nucleotide diversity, and lower genetic distance within populations. These findings provided evidence for understanding the molecular characteristics of M. strigata population expansion in China.

Public acceptance of genetically modified crops engineered with Bacillus thuringiensis (Bt) insecticidal protein genes (BT-GMCs), which confer resistance to various lepidopteran insect pests, is generally lacking. As a major concern over BT-GMCs is the allergenicity of insecticidal proteins, alleviating safety concerns should help increase public acceptance. In this study, three lepidopteran-specific Bt toxins, Cry1Aa, Cy1Ab, and Cry1Ac, were treated with simulated digestive fluids under various conditions. Western blotting using antiserum raised against individual segments (α-helices of domain I and β-sheets of domains II and III) of Cry1Aa showed that digestion produces a variety of polypeptides. In particular, the transmembrane α4-α5 of domain I, which may retain the ability to form pores, was the most resistant to digestion. Intact Cry1A toxins and these digests were then applied to RBL-2H3 cultured rat mast cells to determine whether the toxins directly induce histamine release. However, fluorescence microscopy revealed no specific binding of Cry1A toxins to RBL-2H3 cultured rat mast cells. In addition, neither the OPA method nor HPLC analysis detected significant histamine release from mast cells treated with Cry1A toxins and these digests. Our results provide important data supporting the safety of Cry1A toxins and potentially BT-GMCs.

In this study, we investigated Acridoidea species and their populations in the grasslands managed by 13 divisions of the Xinjiang Production and Construction Corps (hereafter referred to as the Corps) during 2022-2024. We analyzed how the Corps' grassland habitats influenced Acridoidea diversity. Using the netting method, we collected 5290 Acridoidea specimens, representing eight families, 37 genera, and 83 species. The Acridoidea were most abundant in mountain meadows and temperate grasslands, with 42 and 43 species, respectively, while the species richness in temperate desert grasslands and temperate steppe desert grasslands was comparably high. The highest diversity index of Acridoidea was recorded in temperate grasslands (3.053), followed by mountain meadows (2.563). Regarding altitude, the diversity index was highest in the 1000-1500 m range (3.237), followed by 500-1000 m (2.976) and 1500-2000 m (2.867). However, above 1500 m, the diversity index began to decline. The Acridoidea species diversity was significantly positively correlated with the average temperatures of the wettest and warmest seasons, as well as with precipitation levels during the wettest and driest months. The dominant grassland Acridoidea species were primarily distributed in areas with low-to-medium-low soil erodibility. These findings indicate that grassland types, elevation, temperature, humidity, and soil erodibility significantly influence Acridoidea habitats, survival, reproduction, and feeding behaviors, ultimately shaping their composition, distribution, and abundance across different habitats.

Chronic kidney disease (CKD) is a global health concern caused by conditions such as hypertension, diabetes, hyperlipidemia, and chronic nephritis, leading to structural and functional kidney injury. Kidney fibrosis is a common outcome of CKD progression, with abnormal fatty acid oxidation (FAO) disrupting renal energy homeostasis and leading to functional impairments. This results in maladaptive repair mechanisms and the secretion of profibrotic factors, and exacerbates renal fibrosis. Understanding the molecular mechanisms of renal fibrosis is crucial for delaying CKD progression. Ferroptosis is a type of discovered an iron-dependent lipid peroxidation-regulated cell death. Notably, Ferroptosis contributes to tissue and organ fibrosis, which is correlated with the degree of renal fibrosis. This study aims to clarify the complex mechanisms of ferroptosis in renal parenchymal cells and explore how ferroptosis intervention may help alleviate renal fibrosis, particularly by addressing the gap in CKD mechanisms related to abnormal lipid metabolism under the ferroptosis context. The goal is to provide a new theoretical basis for clinically delaying CKD progression.