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Impact of green synthesized zinc oxide nanoparticles for treating dry rot in potato tubers 绿色合成氧化锌纳米粒子对处理马铃薯块茎干腐病的影响
Pub Date : 2024-02-09 DOI: 10.15376/biores.19.2.2106-2119
Mohamed A. Abdelhady, Tarek M. Abdelghany, Sonya H. Mohamed, Salah A. Abdelbary
Potato (Solanum tuberosum) crops have experienced a 22% reduction due to attacks from microbial pathogens and pests. Dry rot disease occurred from risks in El-Minya governorate, Egypt, where potato is the major cultivated crop. Potato cultivar ‘Cara’ samples were collected from the markets of six regions in El-Minya for isolation. They were also tested for the application of biosynthesized zinc oxide nanoparticles (ZnONPs) for disease management. The ability of Exserohilum rostratum to synthesize ZnONPs was documented via UV-visible, X-ray diffraction, Fourier transform infrared spectrum, and transmission and scanning electron microscopy. Spherical shape and crystallite small size (51.0 ± 3.0 nm) were attributed to the created ZnONPs. The ZnONPs were applied in the bio-control of the causative agent (Fusarium nygamai) of dry rot disease and large economic loss of potato cv. ‘Cara’ productivity. The mycosynthesized ZnONPs by E. rostratum at 100 μg/ mL (3 mM) showed antifungal activity against F. nygamai with higher reducing value of mycelium growth diameter 2.0 ± 0.14 cm compared to Revanol 50%, traditional product with a diameter of 3.85 ± 0.10 cm. Overall, ZnONPs are an excellent agent that can be applied to repress the most common fungal disease of potato.
由于微生物病原体和害虫的侵袭,马铃薯(Solanum tuberosum)作物产量减少了 22%。马铃薯是埃及 El-Minya 省的主要种植作物,该省的干腐病发生风险较高。从 El-Minya 六个地区的市场上收集了马铃薯栽培品种 "Cara "的样本进行分离。还对生物合成的氧化锌纳米粒子(ZnONPs)在病害防治中的应用进行了测试。通过紫外可见光、X 射线衍射、傅立叶变换红外光谱以及透射和扫描电子显微镜,证明了 Exserohilum rostratum 合成 ZnONPs 的能力。合成的 ZnONPs 呈球形,结晶尺寸较小(51.0 ± 3.0 nm)。这些 ZnONPs 被应用于生物防治干腐病的病原菌(镰刀菌),对马铃薯品种 "Cara "的产量造成了巨大的经济损失。E. rostratum 真菌合成的 ZnONPs 在 100 μg/ mL (3 mM) 的浓度下对 F. nygamai 具有抗真菌活性,与传统产品 Revanol 50%(直径为 3.85 ± 0.10 厘米)相比,其菌丝生长直径减少值更高,为 2.0 ± 0.14 厘米。总之,ZnONPs 是一种可用于抑制马铃薯最常见真菌病害的优良制剂。
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引用次数: 0
Are biological pretreatments of lignocellulosic residues a real option for biofuels production? 木质纤维素残渣的生物预处理是生物燃料生产的真正选择吗?
Pub Date : 2024-02-07 DOI: 10.15376/biores.19.2.albafierro
Carlos Antonio Alba Fierro, Miguel Ángel Escobedo Bretado, D. M. Núñez Ramírez, María Angélica Martell Nevárez, Francisco Javier Ríos Fránquez
The use of lignocellulosic residues as feedstocks for biofuels production represents an economic and ecofriendly option, since they are generated as byproducts or wastes from different industrial areas. Nevertheless, a pretreatment method aimed at eliminating the lignin content of these residues must be performed. This is required in order to increase cellulose bioavailability, which favors the production of reducing sugars through microbial or enzymatic attack. Some performed pretreatments can be classified as physical, chemical, and physicochemical methods. Although such methods are the most used pretreatments, they are expensive and generate or make use of harmful compounds. Biological methods, by the action of microorganisms or their enzymes for lignin content reduction, may be regarded as an alternative, being cheaper and more friendly to the environment than the aforementioned methods. However, until now, biological pretreatments have not shown the same yield as the previously mentioned methods in both sugar recovery and biofuel production. In that sense, the aim of this work is to review the efficiency of these methods, with the goal of clarifying their advantages and disadvantages for improvement of biofuel production.
使用木质纤维素残渣作为生物燃料生产的原料是一种经济且环保的选择,因为它们是不同工业领域产生的副产品或废物。然而,必须采用一种预处理方法来消除这些残留物中的木质素含量。这样做的目的是提高纤维素的生物利用率,从而有利于通过微生物或酶的作用产生还原糖。有些预处理方法可分为物理、化学和物理化学方法。虽然这些方法是最常用的预处理方法,但成本高昂,而且会产生或使用有害化合物。通过微生物或其酶的作用来降低木质素含量的生物方法可被视为一种替代方法,它比上述方法更便宜,对环境也更友好。然而,到目前为止,生物预处理在糖分回收和生物燃料生产方面还没有显示出与上述方法相同的产量。从这个意义上说,这项工作的目的是审查这些方法的效率,以明确它们在提高生物燃料生产方面的优缺点。
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引用次数: 0
Are biological pretreatments of lignocellulosic residues a real option for biofuels production? 木质纤维素残渣的生物预处理是生物燃料生产的真正选择吗?
Pub Date : 2024-02-07 DOI: 10.15376/biores.19.2.albafierro
Carlos Antonio Alba Fierro, Miguel Ángel Escobedo Bretado, D. M. Núñez Ramírez, María Angélica Martell Nevárez, Francisco Javier Ríos Fránquez
The use of lignocellulosic residues as feedstocks for biofuels production represents an economic and ecofriendly option, since they are generated as byproducts or wastes from different industrial areas. Nevertheless, a pretreatment method aimed at eliminating the lignin content of these residues must be performed. This is required in order to increase cellulose bioavailability, which favors the production of reducing sugars through microbial or enzymatic attack. Some performed pretreatments can be classified as physical, chemical, and physicochemical methods. Although such methods are the most used pretreatments, they are expensive and generate or make use of harmful compounds. Biological methods, by the action of microorganisms or their enzymes for lignin content reduction, may be regarded as an alternative, being cheaper and more friendly to the environment than the aforementioned methods. However, until now, biological pretreatments have not shown the same yield as the previously mentioned methods in both sugar recovery and biofuel production. In that sense, the aim of this work is to review the efficiency of these methods, with the goal of clarifying their advantages and disadvantages for improvement of biofuel production.
使用木质纤维素残渣作为生物燃料生产的原料是一种经济且环保的选择,因为它们是不同工业领域产生的副产品或废物。然而,必须采用一种预处理方法来消除这些残留物中的木质素含量。这样做的目的是提高纤维素的生物利用率,从而有利于通过微生物或酶的作用产生还原糖。有些预处理方法可分为物理、化学和物理化学方法。虽然这些方法是最常用的预处理方法,但成本高昂,而且会产生或使用有害化合物。通过微生物或其酶的作用来降低木质素含量的生物方法可被视为一种替代方法,它比上述方法更便宜,对环境也更友好。然而,到目前为止,生物预处理在糖分回收和生物燃料生产方面还没有显示出与上述方法相同的产量。从这个意义上说,这项工作的目的是审查这些方法的效率,以明确它们在提高生物燃料生产方面的优缺点。
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引用次数: 0
Assessment of the ability for early detection of newly hatched larvae of Hylotrupes bajulus L. using the acoustic emission method in Scots pine wood 使用声发射法评估苏格兰松木中新孵化幼虫 Hylotrupes bajulus L. 的早期检测能力
Pub Date : 2024-02-07 DOI: 10.15376/biores.19.2.2092-2105
A. Krajewski, Piotr Bilski, P. Witomski, Piotr Bobiński
The acoustic emission analysis method was used to determine the activity of very young Hylotrupes bajulus larvae in their long-term development. In the laboratory conditions the simulated scenario of a fresh, intense infestation inside the construction wood was evaluated. The sounds generated by the insects during their feeding on the wooden samples were processed by the measurement system, which was able to detect and count the larva-originated sound pulses and calculate related energy. So far, no one has examined possibilities of detecting infant larvae of Hylotrupes bajulus L. This work is a continuation of research on the influence of the insect mass on its AE-based detection. The experiments performed with multiple wood samples analyzed through the period of one year have shown dependency between the mass of the larva and the intensity of the sound generated by them. The moment of the earliest possible detection of infestation in the wooden structure was evaluated. In the appropriate conditions (large number of young larvae inside the wood and optimal temperature) the insects reach the mass of tens of mg, which makes them detectable using the AE method.
声发射分析方法用于确定巴氏木虱幼虫在长期发育过程中的活动情况。在实验室条件下,对建筑木材内部新发生的严重虫害进行了模拟评估。昆虫在木质样本上取食时发出的声音由测量系统进行处理,该系统能够检测和计算幼虫发出的声音脉冲并计算相关能量。迄今为止,还没有人研究过检测 Hylotrupes bajulus L 幼虫的可能性。通过对多个木材样本进行为期一年的分析,实验结果表明幼虫的质量与其产生的声音强度之间存在依赖关系。对木结构中最早可能发现虫害的时刻进行了评估。在适当的条件下(木材内有大量幼虫和最佳温度),昆虫的质量达到数十毫克,因此可以使用声发射法检测到它们。
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引用次数: 0
Assessment of the ability for early detection of newly hatched larvae of Hylotrupes bajulus L. using the acoustic emission method in Scots pine wood 使用声发射法评估苏格兰松木中新孵化幼虫 Hylotrupes bajulus L. 的早期检测能力
Pub Date : 2024-02-07 DOI: 10.15376/biores.19.2.2092-2105
A. Krajewski, Piotr Bilski, P. Witomski, Piotr Bobiński
The acoustic emission analysis method was used to determine the activity of very young Hylotrupes bajulus larvae in their long-term development. In the laboratory conditions the simulated scenario of a fresh, intense infestation inside the construction wood was evaluated. The sounds generated by the insects during their feeding on the wooden samples were processed by the measurement system, which was able to detect and count the larva-originated sound pulses and calculate related energy. So far, no one has examined possibilities of detecting infant larvae of Hylotrupes bajulus L. This work is a continuation of research on the influence of the insect mass on its AE-based detection. The experiments performed with multiple wood samples analyzed through the period of one year have shown dependency between the mass of the larva and the intensity of the sound generated by them. The moment of the earliest possible detection of infestation in the wooden structure was evaluated. In the appropriate conditions (large number of young larvae inside the wood and optimal temperature) the insects reach the mass of tens of mg, which makes them detectable using the AE method.
声发射分析方法用于确定巴氏木虱幼虫在长期发育过程中的活动情况。在实验室条件下,对建筑木材内部新发生的严重虫害进行了模拟评估。昆虫在木质样本上取食时发出的声音由测量系统进行处理,该系统能够检测和计算幼虫发出的声音脉冲并计算相关能量。迄今为止,还没有人研究过检测 Hylotrupes bajulus L 幼虫的可能性。通过对多个木材样本进行为期一年的分析,实验结果表明幼虫的质量与其产生的声音强度之间存在依赖关系。对木结构中最早可能发现虫害的时刻进行了评估。在适当的条件下(木材内有大量幼虫和最佳温度),昆虫的质量达到数十毫克,因此可以使用声发射法检测到它们。
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引用次数: 0
Gendang Melayu Sarawak (GMS) – Sarawak Malay Drum, the dying and forgotten tradition Gendang Melayu Sarawak(GMS)--沙捞越马来鼓,濒临消亡和被遗忘的传统
Pub Date : 2024-02-06 DOI: 10.15376/biores.19.2.2065-2076
A. E. Sinin, S. Hamdan, K. A. Mohamad Said, Santrol Abdullah, A. F. Musib
This work was conducted using the Picoscope signal extraction procedure, which revealed significant insights regarding the belian wood and its application in Gendang Melayu Sarawak (GMS) production. The amplitude of belian wood GMS signal remains constant, allowing it to sustain its timbre for a longer duration compared to durian wood GMS using the same procedure. Considering that the dimensions of the big belian (BB) and big durian (BD) GMS are almost the same, both GMS yield almost the same note, i.e. G1# (51.9 Hz). Considering that the dimensions of both the small belian (SB) and small durian (SD) GMS are almost the same, both GMS yield almost similar note, i.e. F3 (174 Hz) and E3 (164 Hz). Although both BB and BD showed consistent harmonics, BD only displays 2 harmonics. The SB and SD both display consistent harmonics. Both BB and BD showed pleasing tonal qualities. These occurred due to the closeness of the principal overtones to the consonant interval.
这项工作是利用 Picoscope 信号提取程序进行的,该程序揭示了有关榴莲木及其在砂拉越甘当马来语(GMS)生产中的应用的重要信息。与使用相同程序的榴莲木 GMS 相比,大榴莲木 GMS 信号的振幅保持恒定,使其音色的持续时间更长。考虑到大榴莲(BB)和大榴莲(BD)GMS 的尺寸几乎相同,两种 GMS 产生的音几乎相同,即 G1#(51.9 Hz)。考虑到小榴莲(SB)和小榴莲(SD)GMS 的尺寸几乎相同,两种 GMS 产生的音几乎相似,即 F3(174 赫兹)和 E3(164 赫兹)。虽然 BB 和 BD 都显示出一致的谐波,但 BD 只显示出 2 个谐波。SB 和 SD 都显示出一致的谐波。BB 和 BD 都显示出悦耳的音质。这是因为主泛音与辅音音程很接近。
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引用次数: 0
Gendang Melayu Sarawak (GMS) – Sarawak Malay Drum, the dying and forgotten tradition Gendang Melayu Sarawak(GMS)--沙捞越马来鼓,濒临消亡和被遗忘的传统
Pub Date : 2024-02-06 DOI: 10.15376/biores.19.2.2065-2076
A. E. Sinin, S. Hamdan, K. A. Mohamad Said, Santrol Abdullah, A. F. Musib
This work was conducted using the Picoscope signal extraction procedure, which revealed significant insights regarding the belian wood and its application in Gendang Melayu Sarawak (GMS) production. The amplitude of belian wood GMS signal remains constant, allowing it to sustain its timbre for a longer duration compared to durian wood GMS using the same procedure. Considering that the dimensions of the big belian (BB) and big durian (BD) GMS are almost the same, both GMS yield almost the same note, i.e. G1# (51.9 Hz). Considering that the dimensions of both the small belian (SB) and small durian (SD) GMS are almost the same, both GMS yield almost similar note, i.e. F3 (174 Hz) and E3 (164 Hz). Although both BB and BD showed consistent harmonics, BD only displays 2 harmonics. The SB and SD both display consistent harmonics. Both BB and BD showed pleasing tonal qualities. These occurred due to the closeness of the principal overtones to the consonant interval.
这项工作是利用 Picoscope 信号提取程序进行的,该程序揭示了有关榴莲木及其在砂拉越甘当马来语(GMS)生产中的应用的重要信息。与使用相同程序的榴莲木 GMS 相比,大榴莲木 GMS 信号的振幅保持恒定,使其音色的持续时间更长。考虑到大榴莲(BB)和大榴莲(BD)GMS 的尺寸几乎相同,两种 GMS 产生的音几乎相同,即 G1#(51.9 Hz)。考虑到小榴莲(SB)和小榴莲(SD)GMS 的尺寸几乎相同,两种 GMS 产生的音几乎相似,即 F3(174 赫兹)和 E3(164 赫兹)。虽然 BB 和 BD 都显示出一致的谐波,但 BD 只显示出 2 个谐波。SB 和 SD 都显示出一致的谐波。BB 和 BD 都显示出悦耳的音质。这是因为主泛音与辅音音程很接近。
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引用次数: 0
The study of children’s preferences for the design elements of learning desks based on AHP-QCA 基于 AHP-QCA 的儿童对学习桌设计要素偏好的研究
Pub Date : 2024-02-05 DOI: 10.15376/biores.19.2.2045-2066
Yanfeng Miao, Shuqi Yan, Wei Xu
Children’s study tables are an integral part of a child’s learning life. Consumers are often attracted to their styling when deciding on the use and purchase of a children’s study desk. This study focused on consumption preference for the styling of children’s study desks and delved into the factors that influence these preferences. An important aspect of this research is to understand how different shapes of children’s desks influence consumer preferences through morphological analysis. The study breaks down these desks into six different parts based on morphological analysis: backplane, bookshelves, desktop, cabinet, drawer, and table legs. Through a hierarchical analysis (AHP) and pairwise comparisons, the study created a hierarchy of preferred morphological elements. The hierarchy ranked the importance of each element in influencing consumption preference, revealing the order of preference from backsplash to table legs. In addition, by integrating personal interviews and employing Qualitative Comparative Analysis (QCA), this study provides insights into the most preferred components – backplane and bookshelves. This integrated approach revealed a preference for desks with curved backsplashes and multi-tiered functional shelves, which was ultimately validated by successfully combining weighted rankings of specific component styles.
儿童学习桌是儿童学习生活中不可或缺的一部分。消费者在决定使用和购买儿童学习桌时,往往会被其造型所吸引。本研究重点关注消费者对儿童学习桌造型的偏好,并深入研究影响这些偏好的因素。这项研究的一个重要方面是通过形态分析了解不同形状的儿童书桌如何影响消费者的偏好。研究根据形态分析将这些书桌分成六个不同的部分:背板、书架、桌面、柜子、抽屉和桌腿。通过层次分析法(AHP)和配对比较法,研究建立了首选形态元素的层次结构。该层次结构对每个元素在影响消费偏好方面的重要性进行了排序,揭示了从背板到桌腿的偏好顺序。此外,通过整合个人访谈和采用定性比较分析法(QCA),本研究深入了解了最受偏好的组件--背板和书架。这种综合方法揭示了人们对带有弧形背板和多层功能书架的书桌的偏好,并通过成功结合特定组件风格的加权排名,最终验证了这一偏好。
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引用次数: 0
Culture of Trichoderma sp. with biochar to produce high-activity cellulase in a laboratory 在实验室用生物炭培养毛霉以生产高活性纤维素酶
Pub Date : 2024-02-05 DOI: 10.15376/biores.19.2.2029-2044
Seongwoo Myeong, Jeonghee Yun
Biochar (BC) was used in Trichoderma sp. culture to produce high-activity cellulase on a laboratory scale. The biochar was added into the flask before being applied to the fermenter to identify the enhancement effect and to determine the best amount of addition and the most suitable incubation period. Cellulase production was performed with a working volume of 4 L, and enzymatic hydrolysis was conducted to evaluate the saccharification ability of the enzyme. During incubation, the activities of three enzymes (Endoglucanase (EG), β-glucosidase (BGL), and cellobiohydrolase (CBH)) were measured for three days, and the cellulase activity was determined using a filter paper unit (FPU). In flask scale, EG, BGL, and CBH activities were increased by 1.4, 2.1, and 1.8 folds, respectively, and the incubation period was shortened by adding BC. In the fermenter scale, EG, BGL, and CBH activities were noticeably enhanced by 12.1, 5.8, and 7.2 folds, respectively, and FPU was 42.1 (9.8 folds). Additionally, the conversion rates of cellulose and steam exploded softwood and hardwood were 109.4%, 75.4%, and 87.3%, which were similar to a commercial enzyme (Cellic CTecⅡ). This study demonstrated that biochar could be used to produce high-activity cellulase in a shorter period and suggests a novel method for effective cellulase production.
生物炭(BC)用于毛霉培养,在实验室规模上产生高活性纤维素酶。在将生物炭加入发酵罐之前,先将其加入烧瓶中,以确定生物炭的增强效果,并确定最佳添加量和最合适的培养期。纤维素酶生产的工作容积为 4 升,通过酶水解来评估酶的糖化能力。在培养过程中,连续三天测量三种酶(内切葡聚糖酶(EG)、β-葡萄糖苷酶(BGL)和纤维素水解酶(CBH))的活性,并使用滤纸装置(FPU)测定纤维素酶活性。在烧瓶规模中,EG、BGL 和 CBH 的活性分别提高了 1.4、2.1 和 1.8 倍,加入 BC 缩短了培养时间。在发酵罐规模中,EG、BGL 和 CBH 活性分别明显提高了 12.1、5.8 和 7.2 倍,FPU 为 42.1(9.8 倍)。此外,纤维素和蒸汽引爆软木和硬木的转化率分别为 109.4%、75.4% 和 87.3%,与商业酶(Cellic CTecⅡ)相似。这项研究表明,生物炭可用于在较短时间内生产高活性纤维素酶,并提出了一种有效生产纤维素酶的新方法。
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引用次数: 0
The study of children’s preferences for the design elements of learning desks based on AHP-QCA 基于 AHP-QCA 的儿童对学习桌设计要素偏好的研究
Pub Date : 2024-02-05 DOI: 10.15376/biores.19.2.2045-2066
Yanfeng Miao, Shuqi Yan, Wei Xu
Children’s study tables are an integral part of a child’s learning life. Consumers are often attracted to their styling when deciding on the use and purchase of a children’s study desk. This study focused on consumption preference for the styling of children’s study desks and delved into the factors that influence these preferences. An important aspect of this research is to understand how different shapes of children’s desks influence consumer preferences through morphological analysis. The study breaks down these desks into six different parts based on morphological analysis: backplane, bookshelves, desktop, cabinet, drawer, and table legs. Through a hierarchical analysis (AHP) and pairwise comparisons, the study created a hierarchy of preferred morphological elements. The hierarchy ranked the importance of each element in influencing consumption preference, revealing the order of preference from backsplash to table legs. In addition, by integrating personal interviews and employing Qualitative Comparative Analysis (QCA), this study provides insights into the most preferred components – backplane and bookshelves. This integrated approach revealed a preference for desks with curved backsplashes and multi-tiered functional shelves, which was ultimately validated by successfully combining weighted rankings of specific component styles.
儿童学习桌是儿童学习生活中不可或缺的一部分。消费者在决定使用和购买儿童学习桌时,往往会被其造型所吸引。本研究重点关注消费者对儿童学习桌造型的偏好,并深入研究影响这些偏好的因素。这项研究的一个重要方面是通过形态分析了解不同形状的儿童书桌如何影响消费者的偏好。研究根据形态分析将这些书桌分成六个不同的部分:背板、书架、桌面、柜子、抽屉和桌腿。通过层次分析法(AHP)和配对比较法,研究建立了首选形态元素的层次结构。该层次结构对每个元素在影响消费偏好方面的重要性进行了排序,揭示了从背板到桌腿的偏好顺序。此外,通过整合个人访谈和采用定性比较分析法(QCA),本研究深入了解了最受偏好的组件--背板和书架。这种综合方法揭示了人们对带有弧形背板和多层功能书架的书桌的偏好,并通过成功结合特定组件风格的加权排名,最终验证了这一偏好。
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引用次数: 0
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