Variations in precipitation related to climate change affect soil carbon cycling processes in terrestrial ecosystems, particularly soil respiration (Rs). However, how Rs and its components-heterotrophic respiration (Rh) and autotrophic respiration (Ra)-respond to precipitation changes remains largely unclear in alpine ecosystems, given their distinct substrate sources and biological processes. In this study, we investigated the effects of altered precipitation levels on Rs and its components through a 3-year field experiment, where precipitation was adjusted by ±50 % in the alpine meadows of the northeastern Qinghai-Tibetan Plateau (QTP). Our results showed that precipitation variability did not significantly affect total Rs, but it increased Ra and decreased Rh, leading to a stable overall Rs. Specifically, increased precipitation (IP) and decreased precipitation (DP) reduced Rh by 22.75 % and 20.60 %, respectively, while Ra was elevated by 56.39 % and 40.24 % compared to the control (CK). Regression analysis revealed a significant exponential relationship between Rs and temperature. Both IP and DP treatments reduced the temperature sensitivity (Q10) of Rs and its components compared to CK, suggesting that deviations from typical moisture levels suppress the response of Rs to temperature changes. The direct negative effect of IP on Rs was mitigated by a positive indirect effect through fungal richness, while DP produced opposite indirect effects via Rh and Ra, resulting in a weak overall impact on Rs. These site-specific results reveal the different responses of Ra and Rh to changing precipitation and suggest that extreme changes in precipitation impact soil microbial richness, suppress Rh, and weaken the decomposition and release of soil organic carbon in alpine meadows on the QTP.
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