The disturbance wave caused by excavation or blasting of underground surrounding rock causes fatigue degradation effect of rock and eventually leads to disasters. However, the fatigue damage characteristics and fatigue models of rock under true triaxial disturbance are scare. Therefore, a series of true triaxial disturbance tests were conducted to investigate the rock fatigue deformation, strength and damage behaviors under different conditions. The evolutions of static damage and fatigue damage are separated and investigated respectively. Fatigue deformation and damage of rock under true triaxial stress undergoes three stages: attenuation, constant velocity and acceleration stage. The crack initiation stress can be as the initial condition of the fatigue deformation; the fatigue critical stress σdc of rock entering the acceleration failure stage was proposed and explored, with increasing frequency, σdc increase slightly and with increasing σ2, σdc increase obviously. Then, a novel fractional fatigue mechanical model considering the fatigue damage and intermediate principal stress effects of rock under true triaxial disturbance was proposed. The theoretical results of the model agree well with the results of the tests. Finally, the sensitivity analysis of stresses and model parameters and the model predictions under other untesting conditions were carried out to improve the understanding and prediction level of fatigue failure in underground engineering.