Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献_第8页

Regulatory variants of glycogen synthase contribute to differential glycogen content between Crassostrea gigas and C. sikamea 糖原合成酶的调节变异导致长牡蛎和狐獴之间糖原含量的差异

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-26 DOI: 10.1016/j.cbpb.2025.111101

Ying Tan , Chenyu Shi , Qi Li , Hongqiang Xu , Shikai Liu

In marine bivalves, glycogen functions as a key energy source, influencing essential physiological processes such as growth, development, gametogenesis, and the reproductive cycle. Glycogen synthase (GYS), the key enzyme regulating glycogen biosynthesis, shows expression patterns directly correlated with seasonal glycogen fluctuations. This study identified genetic variations within the transcriptional regulatory region of the GYS gene in populations of Crassostrea gigas and C. sikamea. Two key single nucleotide polymorphism (SNP) sites, SNP -792-G/T and SNP -60-T/C, were identified to be associated with glycogen content. The polymorphisms in the GYS gene promoter result in species-specific differences in transcriptional binding activity, with C. sikamea showing higher activity in core region compared to C. gigas. Polymorphisms in this highly conserved promoter region alter the affinity of transcription regulatory elements for hypoxia-inducible factor 1 alpha (HIF-1α) and octamer-binding transcription factor 1 (OCT-1), thereby influencing GYS gene expression levels. These changes were related with interspecific differences in glycogen content between C. sikamea and C. gigas. Experimental evidence confirmed the specific binding of transcription factors to SNP-mutated promoter motifs. These SNPs represent critical species-specific regulatory sites and may serve as valuable markers for selecting oyster strains with high glycogen content. Our findings demonstrate that SNPs within the transcription regulatory elements of the GYS gene alter the binding affinity for transcription factors OCT-1 and HIF-1α, thereby playing a critical role in energy metabolism in oysters. Our study offers novel insights into the transcriptional regulation of the GYS gene and its contribution to glycogen storage.

在海洋双壳类动物中，糖原是一种重要的能量来源，影响着生长、发育、配子体发生和生殖周期等重要生理过程。糖原合成酶（GYS）是调节糖原生物合成的关键酶，其表达模式与糖原的季节波动直接相关。本研究确定了长牡蛎和狐蝠种群中GYS基因转录调控区域内的遗传变异。两个关键的单核苷酸多态性位点SNP -792-G/T和SNP -60-T/C与糖原含量相关。GYS基因启动子的多态性导致了物种特异性转录结合活性的差异，与C. gigas相比，C. sikamea在核心区域表现出更高的活性。这个高度保守的启动子区域的多态性改变了转录调控元件对缺氧诱导因子1α (HIF-1α)和八聚体结合转录因子1 （OCT-1）的亲和力，从而影响GYS基因的表达水平。这些变化与狐獴和巨蝉种间糖原含量的差异有关。实验证据证实了转录因子与snp突变的启动子基序的特异性结合。这些snp代表了关键的物种特异性调控位点，可能作为选择高糖原含量牡蛎菌株的有价值的标记。我们的研究结果表明，GYS基因转录调控元件内的snp改变了转录因子OCT-1和HIF-1α的结合亲和力，从而在牡蛎的能量代谢中发挥关键作用。我们的研究为GYS基因的转录调控及其对糖原储存的贡献提供了新的见解。

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引用次数: 0

Effects of dietary oregano essential oil on digestive tissue structure and function, antioxidant and immune responses and gut microbiota of red swamp crayfish (Procambarus clarkii) 饲粮中添加牛至精油对克氏原螯虾消化组织结构和功能、抗氧化和免疫反应及肠道微生物群的影响

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-21 DOI: 10.1016/j.cbpb.2025.111100

Xinzi Guo , Zhaoxuan Zhu , Hanxiao Wang , Yue Chen , Songrun Zhang , Dian Wang , Xingxing Fang , Ping Yang

Modifying the diet is an effective strategy to enhance the production and health of farmed red swamp crayfish (Procambarus clarkii). In this study the effects of adding dietary oregano essential oil (OEO) at 0, 100, 300, and 500 mg/kg concentrations were investigated in red swamp crayfish after a 3-week feeding trial. Histology showed no negative impacts on the structural integrity of the hepatopancreas and intestine. Total protease activity in the hepatopancreas, hemolymph alkaline and acid phosphatase activity, hemolymph superoxide dismutase activity and hepatopancreas glutathione levels were higher in the 500 mg/kg OEO group than in the 0 mg/kg group. No significant differences were observed in hepatopancreas phenoloxidase activity or hemolymph malondialdehyde content between treatment groups. Supplementing the diet with OEO upregulated the expression of antioxidant and immune-related genes in the hepatopancreas including metallothionein, gamma interferon-inducible lysosomal thiol reductase, and catalase in the 500 mg/kg OEO group relative to the 0 mg/kg control. Ferritin gene expression was unaltered. The intestinal microbiota of crayfish fed OEO showed an increased relative abundance of Proteobacteria and Actinobacteriota, and a lower abundance of Firmicutes. This study demonstrates the potential of OEO as a novel feed additive for enhancing antioxidant capacity and the immune response in red swamp crayfish. This research addresses the growing need for sustainable aquaculture practices and highlights the application of plant-derived bioactivities in improving crustacean health.

改变饲料是提高克氏原螯虾（Procambarus clarkii）产量和健康的有效策略。本试验通过3周的饲养试验，研究了在红沼泽小龙虾中添加0、100、300和500 mg/kg浓度的牛至精油（OEO）的效果。组织学显示对肝胰腺和肠的结构完整性没有负面影响。500 mg/kg OEO组肝胰脏总蛋白酶活性、血淋巴碱性和酸性磷酸酶活性、血淋巴超氧化物歧化酶活性和肝胰脏谷胱甘肽水平均高于0 mg/kg组。肝胰腺酚氧化酶活性和血淋巴丙二醛含量在治疗组间无显著差异。与0 mg/kg对照组相比，500 mg/kg OEO组肝胰腺中金属硫蛋白、γ干扰素诱导溶酶体硫醇还原酶和过氧化氢酶等抗氧化和免疫相关基因的表达上调。铁蛋白基因表达未见改变。小龙虾肠道菌群中变形菌门和放线菌门的相对丰度增加，厚壁菌门的相对丰度降低。本研究证明了OEO作为一种新型饲料添加剂在提高红沼泽小龙虾抗氧化能力和免疫应答方面的潜力。本研究解决了对可持续水产养殖实践日益增长的需求，并强调了植物源性生物活性在改善甲壳类动物健康方面的应用。

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引用次数: 0

Integrative whole-genome methylation and transcriptome analysis reveals epigenetic modulation of glucose metabolism by dietary berberine in blunt snout bream (Megalobrama amblycephala) 全基因组甲基化和转录组整合分析揭示钝口鳊鱼（Megalobrama amblycephala）膳食小檗碱对葡萄糖代谢的表观遗传调节作用

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-16 DOI: 10.1016/j.cbpb.2025.111098

Xiaoyan Jia, Wei Qiang, Le Chang, Kang Xiao, Ronghua Zhou, Qiyong Qiu, Guangzhen Jiang, Xiangfei Li, Cheng Chi, Wenbin Liu, Dingdong Zhang

The present research was designed to explore the epigenetic mechanism by which dietary berberine (BBR) affects glucose metabolism in fish. Blunt snout bream (Megalobrama amblycephala) is susceptible to disturbances in glucose metabolism when subjected to prolonged high-carbohydrate diets. This study aimed to elucidate whether BBR can enhance glucose regulation in M. amblycephala via modulating DNA methylation levels. Fish (average weight of 20.36 ± 1.44 g) were administered a normal-carbohydrate diet (NC, 30 % carbohydrate), a high-carbohydrate diet (HC, 43 % carbohydrate), or a high-carbohydrate diet supplemented with 50 mg/kg berberine (HB) for 10 weeks. Subsequently, global DNA methylation level, whole-genome bisulfite sequencing (WGBS), RNA-seq, bisulfite sequencing PCR, and real-time quantitative PCR were employed to analyze the DNA methylation patterns and transcription results of the liver genome. The findings indicated that high carbohydrate diets induced glucose metabolism disorders in M. amblycephala, whereas BBR mitigated these metabolic disturbances by reducing methylation levels. WGBS results revealed that CG-type cytosine methylation predominated, and that DNA methylation mainly occurred in promoter, intron, and exon regions. Furthermore, analyses demonstrated a negative correlation between DNA methylation around the transcriptional start site and gene expression levels for 47 genes. Functional enrichment analysis revealed that these genes were associated with 60 KEGG pathways, including 12 genes implicated in the amelioration of insulin resistance, reduction of gluconeogenesis, and maintenance of glucose homeostasis. Consequently, we generated a comprehensive catalog of liver DNA methylation in M. amblycephala, which provides a foundational framework for future investigations into the epigenetic regulation of glucose metabolism by BBR.

本研究旨在探讨饲料中小檗碱（BBR）影响鱼类葡萄糖代谢的表观遗传机制。钝口鲷（Megalobrama amblycephala）在长期高碳水化合物饮食时易受葡萄糖代谢紊乱的影响。本研究旨在阐明BBR是否可以通过调节DNA甲基化水平来增强双头m.s amblycephala的葡萄糖调节。鱼（平均体重20.36±1.44 g）被给予正常碳水化合物饮食（NC， 30%碳水化合物），高碳水化合物饮食（HC， 43%碳水化合物），或高碳水化合物饮食中添加50 mg/kg小檗碱（HB），为期10周。随后，采用全局DNA甲基化水平、全基因组亚硫酸氢盐测序（WGBS）、RNA-seq、亚硫酸氢盐测序PCR和实时定量PCR分析肝脏基因组DNA甲基化模式和转录结果。研究结果表明，高碳水化合物饮食会引起双头m.a blblycephala的糖代谢紊乱，而BBR通过降低甲基化水平来减轻这些代谢紊乱。WGBS结果显示cg型胞嘧啶甲基化占主导地位，DNA甲基化主要发生在启动子、内含子和外显子区域。此外，分析表明47个基因转录起始位点附近的DNA甲基化与基因表达水平呈负相关。功能富集分析显示，这些基因与60个KEGG通路相关，其中12个基因与改善胰岛素抵抗、减少糖异生和维持葡萄糖稳态有关。因此，我们生成了一个全面的双头m.b amblycephala肝脏DNA甲基化目录，这为未来研究BBR对葡萄糖代谢的表观遗传调控提供了基础框架。

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引用次数: 0

Effects of Vibrio alginolyticus on intestinal health and intestinal flora of sea urchin (Strongylocentrotus intermedius) 藻溶弧菌对海胆（Strongylocentrotus intermedius）肠道健康和肠道菌群的影响

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-16 DOI: 10.1016/j.cbpb.2025.111099

Xiaochen Zhang , Wei Chen , Haoyu Zhang , Yan Li , Yijing Han , Wan Liu , Yaqiong Liu , Xiaona Wang , Xuekai Zhang , Deyang Tian , Xiaotong Wang

The aim of this study was to understand the effect of Vibrio alginolyticus on the intestinal tract of Strongylocentrotus intermedius. The effects of injecting V. alginolyticus into the body cavity via the perioral membrane at concentrations of 0 CFU/mL (C), 1.5 × 10⁷ CFU/mL (VA1), and 1.5 × 10⁸ CFU/mL (VA10) on the intestinal tract of S. intermedius were analyzed using histological examination, immunoenzyme activity, and 16S rRNA sequencing. The results showed that V. alginolyticus caused intestinal tissue damage and oxidative stress (e.g. altered levels of superoxide dismutase). In addition, the intestinal flora was altered. At the phylum level, the abundance of Bacteroidota was significantly decreased in the VA10 group, at the genus level, Vibrio spp. exhibited a significant increase following V. alginolyticus injection. Prediction of Kyoto Encyclopedia of Genes and Genomes function in the intestinal flora revealed that high concentrations of V. alginolyticus may have induced pathways such as energy metabolism. These results indicated that V. alginolyticus caused lesions in the intestinal morphology of S. intermedius and disrupted the balance of intestinal flora.

本研究旨在了解溶藻弧菌对中间圆心弧菌肠道的影响。采用组织学检查、免疫酶活性、16S rRNA测序等方法分析了浓度为0 CFU/mL (C)、1.5 × 107 CFU/mL （VA1）、1.5 × 108 CFU/mL （VA10）的溶藻弧菌经口周膜体腔注射对中间链球菌肠道的影响。结果表明，溶藻弧菌引起肠道组织损伤和氧化应激（如超氧化物歧化酶水平改变）。此外，肠道菌群也发生了改变。在门水平上，VA10组拟杆菌门的丰度显著降低；在属水平上，溶藻弧菌组弧菌门的丰度显著增加。《京都基因与基因组百科全书》对肠道菌群功能的预测表明，高浓度的溶藻弧菌可能具有能量代谢等诱导途径。这些结果表明，溶藻弧菌引起了中间葡萄球菌肠道形态的病变，破坏了肠道菌群的平衡。

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引用次数: 0

Effect of prebiotics added to the culture water on the bacterial profiles of biofloc and the gills, hepatopancreas, and intestine of Penaeus vannamei 在培养水中添加益生元对凡纳滨对虾生物群落及鳃、肝胰腺和肠道细菌分布的影响。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-01 DOI: 10.1016/j.cbpb.2025.111097

Misael Rosales-Leija , Delbert M. Gatlin III , Jonathan P. Holt , Addison L. Lawrence

Prebiotics alter microbial communities by supplying carbon to specific bacteria that benefit the host. However, the effect of prebiotics added to the culture water on shrimp production and bacterial composition has not been demonstrated. Therefore, this study evaluated the effects of four prebiotics — short-chain fructooligosaccharide, galactooligosaccharide, inulin, and mannan-oligosaccharide — and two non-prebiotic carbohydrates — sucrose and wheat starch — on shrimp growth, nutrient composition of shrimp muscle and biofloc, biofloc concentration, and bacterial profiles in biofloc and gills, hepatopancreas, and intestine of Penaeus vannamei. Shrimp (6.0 ± 0.5 g) were stocked into 36 tanks (72 shrimp/m²) and raised in a biofloc technology system consisting of two phases: an autotrophic-dominated phase (days 0–4) and a heterotrophic-dominated phase (days 5–26). The prebiotic and non-prebiotic carbohydrates were added to the water at a rate of 3 % of the feed provided. Key findings revealed that adding prebiotics to the water did not affect shrimp growth. Notably, shrimp muscle from the mannan-oligosaccharide treatment exhibited a significantly higher lipid content than that from the galactooligosaccharide treatment (p ≤ 0.05). No significant effect was observed on the nutrient composition of biofloc. Biofloc concentration was lower in the wheat starch treatment (p ≤ 0.05). Finally, the prebiotics altered the bacterial composition of biofloc and shrimp gills, hepatopancreas, and intestine. These results revealed that adding prebiotics to the culture water of a biofloc system has an effect on shrimp quality and microbial communities of biofloc and various shrimp tissues, suggesting their potential benefit on shrimp production and health.

益生素通过向特定细菌提供碳来改变微生物群落，从而使宿主受益。然而，添加到养殖水中的益生元对对虾产量和细菌组成的影响尚未得到证实。因此，本研究评估了四种益生元（短链果寡糖、半乳寡糖、菊粉和甘露寡糖）和两种非益生元碳水化合物（蔗糖和小麦淀粉）对对虾生长、对虾肌肉和生物絮团的营养成分、生物絮团浓度以及生物絮团、对虾鳃、肝胰脏和肠道中细菌特征的影响。将对虾（6.0 ± 0.5 克）放入 36 个水箱（72 只/平方米），在生物絮凝技术系统中养殖，该系统包括两个阶段：自养为主的阶段（第 0-4 天）和异养为主的阶段（第 5-26 天）。益生菌和非益生碳水化合物按所提供饲料的 3% 的比例添加到水中。主要研究结果表明，在水中添加益生素不会影响虾的生长。值得注意的是，来自甘露寡糖处理的对虾肌肉的脂质含量明显高于来自半乳寡糖处理的对虾肌肉的脂质含量（p ≤ 0.05）。生物絮凝物的营养成分没有受到明显影响。小麦淀粉处理的生物絮凝物浓度较低（p ≤ 0.05）。最后，益生素改变了生物絮凝物和对虾鳃、肝胰腺和肠道的细菌组成。这些结果表明，在生物絮团系统的养殖水中添加益生素对对虾的质量以及生物絮团和对虾各种组织的微生物群落有影响，表明益生素对对虾的生产和健康有潜在的益处。

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引用次数: 0

Dietary short-chain fatty acid supplementation does not affect growth performance, metabolism, and oxidative status of European seabass (Dicentrarchus labrax) juveniles 饲料中添加短链脂肪酸不影响欧洲鲈鱼幼鱼的生长性能、代谢和氧化状态。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-27 DOI: 10.1016/j.cbpb.2025.111096

F. Fontinha , N. Martins , R. Magalhães , H. Peres , A. Oliva-Teles

This study investigated the effects of dietary short-chain fatty acid (SCFA) supplementation, on growth performance, metabolism, and antioxidant status of European seabass juveniles. Six isoproteic (43 % crude protein) and isolipidic (18 % crude lipid) diets were formulated to include 0.25 and 0.50 % Sodium acetate (SA), Sodium propionate (SP), or Sodium butyrate (SB). A diet without SCFA supplementation was used as a control. The diets were fed to triplicate groups of European seabass juveniles (initial body weight of 15 g) for 56 days. The supplementation of SCFA in the diet had no impact on the growth, feed utilization, or body composition of seabass. In the intestine, gene expression of pyruvate kinase (pk) and glucokinase (gk), phosphoenolpyruvate carboxykinase (pepck), glucose facilitative carrier type 2 (glut2), and citrate synthase (cs) was lower in fish fed the SP0.50 diet than in the other groups. Moreover, fatty acid synthase (fas) gene expression was lower in fish fed the SA0.25, SA0.50, and SB0.25 diets than in the other groups. Further, catalase (CAT) and glutathione reductase (GR) activity and lipid peroxidation (LPO) levels showed no differences between groups. In contrast, glutathione peroxidase (GPX) activity was higher in fish fed the SP0.50 diet. In the liver, GR activity and LPO levels showed no differences between groups. In contrast, CAT activity was lower in all dietary treatments than in control, and GPX and G6PDH activity was lower in fish fed with the SB (0.25 and 0.50 %) diet than in the other diets. Overall, SCFA supplementation did not affect growth performance and feed utilization and only had minor effects on metabolism and antioxidant defense mechanisms.

本试验研究了饲料中添加短链脂肪酸（SCFA）对欧洲鲈鱼幼鱼生长性能、代谢和抗氧化状态的影响。配制6种异蛋白（43 %粗蛋白质）和等脂（18 %粗脂肪）饲粮，分别为0.25和0.50 %醋酸钠（SA）、丙酸钠（SP）和丁酸钠（SB）。不添加短链脂肪酸的日粮作为对照。将饲料投喂初始体重为15 g的三组欧洲鲈鱼幼鱼，投喂56 d。饲料中添加短链脂肪酸对鲈鱼的生长、饲料利用率和体组成均无影响。在肠道中，SP0.50饲料中丙酮酸激酶（pk）和葡萄糖激酶（gk）、磷酸烯醇丙酮酸羧激酶（pepck）、葡萄糖促进载体2型（glut2）和柠檬酸合成酶（cs）的基因表达量低于其他各组。此外，SA0.25、SA0.50和SB0.25组的脂肪酸合成酶（fas）基因表达量低于其他各组。过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）活性及脂质过氧化（LPO）水平各组间无显著差异。相比之下，SP0.50饲料的谷胱甘肽过氧化物酶（GPX）活性较高。肝脏中GR活性和LPO水平各组间无显著差异。与此相反，各饲料处理的CAT活性均低于对照组，添加SB（0.25和0.50 %）饲料的GPX和G6PDH活性均低于其他饲料。总体而言，添加短链脂肪酸不会影响生长性能和饲料利用率，仅对代谢和抗氧化防御机制有轻微影响。

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引用次数: 0

Effects of acute low-temperature stress on respiratory metabolism, antioxidants, and metabolomics of red swamp crayfish, Procambarus clarkii 急性低温胁迫对克氏原螯虾呼吸代谢、抗氧化剂和代谢组学的影响

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-25 DOI: 10.1016/j.cbpb.2025.111095

Yu Ding , Wenbin Sha , Yunfei Sun , Yongxu Cheng

Crayfish (Procambarus clarkii) aquaculture is threatened by abrupt temperature decreases caused by climatic phenomena, such as cold waves and seasonal fluctuations. In this study, crayfish were exposed to an abrupt temperature change from 17 °C to 7 °C for 24 h to investigate the effects of acute low-temperatures on respiratory metabolism, antioxidants, and metabolomics. The results showed that acute low-temperatures significantly reduced the activities of pyruvate kinase, lactate dehydrogenase, and succinate dehydrogenase in the gills and hemolymph, associated with decreases in anaerobic and aerobic respiratory capacities, and significant decreases in oxygen consumption, ammonia excretion, and maximum metabolic rates. Antioxidant enzymes in the hepatopancreas and hemolymph initially increased then decreased within 24 h. Metabolomics revealed that glycerophospholipid metabolism and glycosylphosphatidylinositol anchor biosynthesis pathways responded to acute low-temperatures, with glycerophospholipids being the most significantly differentially expressed metabolites. These results supported the hypothesis that crayfish exhibit lower metabolic activity at low temperatures. Our data provide mechanistic insight into the biological changes induced by acute low-temperature and may provide insight into culture of P. clarkii in cold waters.

由于寒潮和季节波动等气候现象引起的气温骤降，对克氏原螯虾（Procambarus clarkii）的养殖构成了威胁。本研究将小龙虾置于17°C到7°C的温度突变环境中24 h，研究急性低温对小龙虾呼吸代谢、抗氧化剂和代谢组学的影响。结果表明，急性低温显著降低了鳃和血淋巴中丙酮酸激酶、乳酸脱氢酶和琥珀酸脱氢酶的活性，导致无氧和有氧呼吸能力下降，耗氧量、氨排泄量和最大代谢率显著降低。肝胰脏和血淋巴抗氧化酶在24 h内先升高后降低。代谢组学显示，甘油磷脂代谢和糖基磷脂酰肌醇锚定生物合成途径对急性低温有反应，其中甘油磷脂是差异表达最显著的代谢物。这些结果支持了小龙虾在低温下表现出较低代谢活性的假设。我们的数据为急性低温诱导的生物学变化提供了机制上的见解，并可能为克拉氏杆菌在冷水中的培养提供见解。

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引用次数: 0

Insight into Ca2+- inositol 1,4,5-trisphosphate receptor 2 (IP3R2)-mediated unfolded protein response and apoptosis in scallop Patinopecten yessoensis under high temperature stress 高温胁迫下扇贝Ca2+-肌醇1,4,5-三磷酸受体2 （IP3R2）介导的未折叠蛋白反应和凋亡的研究

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-25 DOI: 10.1016/j.cbpb.2025.111092

Wenfei Gu , Xiaoxue Ma , Chuanyan Yang , Dongli Jiang , Hongmei Fan , Lingling Wang , Linsheng Song

Inositol 1,4,5-trisphosphate receptor 2 (IP₃R2) is an essential Ca²⁺ release channel protein located in the endoplasmic reticulum (ER), and plays a significant role in responding to various environmental stimuli. In the present study, the function of IP₃R2 from Yesso scallop Patinopecten yessoensis (PyIP₃R2) in regulating the Ca²⁺-mediated unfolded protein response (UPR) and apoptosis after high temperature (25 °C) treatment was investigated. Three MIR domains, one RYDR_ITPR domain, one RIH_assoc domain and one Ion_trans domain were identified in PyIP₃R2. Both D-myo-inositol-1,4,5-triphosphate (IP3, an activator of IP₃R) and high temperature significantly upregulated the mRNA expression level of PyIP₃R2 and genes related to apoptosis and the UPR, and also increased intracellular Ca²⁺ content (p < 0.05). Furthermore, the IP₃R antagonist 2-aminoethyl diphenylborinate (2-APB) had the opposite effect, decreasing intracellular Ca²⁺ content and the mRNA expression level of PyIP₃R2, glucose regulated protein 78 (PyGRP78) and PyCaspase-3 (p < 0.05). However, the apoptosis rate and Caspase-3 activity remained comparable to those in the injection control group. These findings indicate that PyIP₃R2 mediates UPR and apoptosis in scallop haemocytes by regulating Ca²⁺content and distribution, and providing insight into the cellular responses of scallops to high temperature.

肌醇1,4,5-三磷酸受体2 （IP3R2）是一种必需的Ca2+释放通道蛋白，位于内质网（ER）中，在响应各种环境刺激中起重要作用。本研究研究了Yesso扇贝（Patinopecten yessoensis）的IP3R2 （PyIP3R2）在高温（25 °C）处理后对Ca2+介导的未折叠蛋白反应（UPR）和细胞凋亡的调控作用。在PyIP3R2中鉴定出3个MIR结构域、1个RYDR_ITPR结构域、1个RIH_assoc结构域和1个Ion_trans结构域。d -肌醇-1,4,5-三磷酸（IP3R的激活剂IP3）和高温均显著上调PyIP3R2、凋亡相关基因和UPR的mRNA表达水平，并增加细胞内Ca2+含量(p 3R拮抗剂2-氨基乙基二苯硼酸盐（2-APB）具有相反的作用，降低细胞内Ca2+含量和PyIP3R2的mRNA表达水平。葡萄糖调节蛋白78 （PyGRP78）和PyCaspase-3 （p 3R2）通过调节Ca2+含量和分布介导扇贝血细胞的UPR和凋亡，并为扇贝对高温的细胞反应提供了见解。

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引用次数: 0

Changes in the biochemical and gene expression profiles of Palaemon carinicauda during ovarian development Palaemon carinicauda卵巢发育过程中生化和基因表达谱的变化。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-18 DOI: 10.1016/j.cbpb.2025.111093

Weitao Cheng , Chao Wang , Dexue Liu , Menghao Fan , Yanrong Cao , Wanyu Han , Tinglu Hu , Guangwei Hu , Huan Gao

Palaemon carinicauda is an economically important shrimp species in China. In this study, muscle, hepatopancreas and ovaries were collected to measure their biochemical composition during ovarian development. In addition, dynamic changes in the transcriptome of P. carinicauda ovaries were investigated. The results showed steady increases in glycogen, triglyceride (TG) and protein levels in the ovaries during maturation. The glycogen level increased consistently in the hepatopancreas, while in the muscle, glycogen showed a downward trend after reaching its highest value at Stage III. Similarly, TG concentration in the hepatopancreas decreased before subsequently increasing, while in the case of muscle, the TG level increased from Stage I to Stage IV prior to a significant decrease in Stage V. In hepatopancreas and muscle, the highest level of protein was observed at Stage III and Stage II respectively and at subsequent stages, the levels decreased, with the lowest value reached in Stage V. Results of RNA-seq revealed dynamic changes in the ovarian transcriptome during development, with extensive changes taking place from Stage I to Stage II and Stage IV to Stage V. KEGG pathway enrichment analysis of upregulated differentially expressed genes (DEGs) revealed that metabolic pathway, lysosome, protein processing in the endoplasmic reticulum, mitogen-activated protein kinase (MAPK) signaling pathway, extracellular matrix (ECM)-receptor interaction, purine metabolism and phagosome were the main enriched pathways. The above results provide a framework for understanding the biochemical and transcriptome dynamics during P. carinicauda ovarian development.

大虾是中国重要的经济虾种。本研究采集了肌肉、肝胰腺和卵巢，测定其在卵巢发育过程中的生化组成。此外，我们还研究了石竹子房转录组的动态变化。结果显示，在成熟过程中，卵巢中的糖原、甘油三酯（TG）和蛋白质水平稳步上升。肝胰腺糖原水平持续升高，而肌肉糖原在III期达到最高值后呈下降趋势。同样，肝胰脏中TG浓度先下降后上升，而肌肉中TG浓度从ⅰ期上升到ⅳ期，然后在ⅴ期显著下降。在肝胰脏和肌肉中，蛋白质水平分别在ⅲ期和ⅱ期达到最高水平，在随后的阶段水平下降。RNA-seq结果显示卵巢转录组在发育过程中发生了动态变化，从I期到II期和IV期到v期发生了广泛的变化。KEGG途径富集分析上调的差异表达基因（DEGs）表明代谢途径、溶酶体、内质网蛋白加工、丝裂原活化蛋白激酶（MAPK）信号通路细胞外基质-受体相互作用、嘌呤代谢和吞噬体是主要富集途径。上述结果为了解斑拟南芥卵巢发育过程中的生化和转录组动力学提供了一个框架。

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引用次数: 0

Editorial: Methods in Comparative Biochemistry and Physiology – Challenges and Opportunities 社论：比较生物化学和生理学的方法——挑战和机遇。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-03-15 DOI: 10.1016/j.cbpb.2025.111094

B.G. Borowiec , W. Joyce , C.N. Glover

引用次数: 0