The rice leaffolder, Cnaphalocrocis medinalis (Guenée), is a major migratory insect pest in paddy fields that damages rice by folding and feeding on leaves, causing chlorophyll loss and resulting in significant yield losses when its population density exceeds an economic threshold. Sustainable pest management requires ‘green plant protection’ solutions. Advances in science and technology have introduced numerous green methods for sustainable management of the rice leaffolder. This paper reviews recent research advancements in rice leaffolder management, such as ecological regulation, healthy cultivation, behavioral regulation, biological control, and rational insecticide application. Based on accurate monitoring and early warning systems, rice leaffolder management can incorporate comprehensive green control products, green control technologies, and control modes. This paper provides prospects for discussing the future of rice leaffolder management, achieving sustainable management of the rice leaffolder, and ensuring rice production safety.
{"title":"Sustainable Management Strategies for Rice Leaffolder, Cnaphalocrocis medinalis (Guenée): Progress and Prospects","authors":"Yajun Yang, Yanhui Lu, Junce Tian, Xusong Zheng, Jiawen Guo, Xiaowei Liu, Zhongxian Lü, Hongxing Xu","doi":"10.1016/j.rsci.2024.12.011","DOIUrl":"10.1016/j.rsci.2024.12.011","url":null,"abstract":"<div><div>The rice leaffolder, <em>Cnaphalocrocis medinalis</em> (Guenée), is a major migratory insect pest in paddy fields that damages rice by folding and feeding on leaves, causing chlorophyll loss and resulting in significant yield losses when its population density exceeds an economic threshold. Sustainable pest management requires ‘green plant protection’ solutions. Advances in science and technology have introduced numerous green methods for sustainable management of the rice leaffolder. This paper reviews recent research advancements in rice leaffolder management, such as ecological regulation, healthy cultivation, behavioral regulation, biological control, and rational insecticide application. Based on accurate monitoring and early warning systems, rice leaffolder management can incorporate comprehensive green control products, green control technologies, and control modes. This paper provides prospects for discussing the future of rice leaffolder management, achieving sustainable management of the rice leaffolder, and ensuring rice production safety.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 322-338"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-01DOI: 10.1016/j.rsci.2024.12.010
Huijuan Li , Haipeng Yu , Guanrong Huang , Zengying Huang , Lu Tang , Pengfei Yang , Zhengzheng Zhong , Guocheng Hu , Peng Zhang , Hanhua Tong
{"title":"LR is a Novel Gene Regulating Amylose Content in Rice Revealed by Genome-Wide Association Study","authors":"Huijuan Li , Haipeng Yu , Guanrong Huang , Zengying Huang , Lu Tang , Pengfei Yang , Zhengzheng Zhong , Guocheng Hu , Peng Zhang , Hanhua Tong","doi":"10.1016/j.rsci.2024.12.010","DOIUrl":"10.1016/j.rsci.2024.12.010","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 277-282"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.rsci.2025.01.004
He Chen , Ruan Yunze , Jia Zhongjun
To improve the yield and quality of rice grown on saline-alkali soil, a meta-analysis combined with micro-district experimental studies was conducted in China to examine the impact of humic acid- based organic fertilizer and chemical fertilizer on rice yield and quality. This study employed a two-factor fully randomized experimental design, incorporating four levels of humic acid (F0, 0.0 g/pot; F1, 4.8 g/pot; F2, 12.0 g/pot; and F3, 19.2 g/pot) and three levels of chemical fertilizer (A1, full conventional dosage; A2, 85% of conventional dosage; and A3, 70% of conventional dosage). The meta-analysis revealed that the application of organic fertilizer (at a rate of 1500‒3000 kg/hm2) combined with chemical fertilizer had a significantly positive effect on the theoretical yield, tiller number, partial factor productivity, and SPAD value of rice. Temperature, organic fertilizer application, and chemical fertilizer levels were identified as critical factors affecting rice yield. The micro-experiments demonstrated that the application of humic acid organic fertilizer with treatment F3 significantly elevated the SPAD value at the full heading and grain filling stages. Increased panicle number and seed-setting rate were the main contributors to the rise in yield, with the F3 treatment yielding the highest overall. The effective leaf area, high-efficiency leaf area, and dry matter accumulation in rice treated with F3 were all higher compared with the F0 treatment. Our findings indicated that the addition of humic acid organic fertilizer can markedly improve the partial factor productivity and agronomic efficiency of rice. In conclusion, the application of F3 organic fertilizer combined with A3 chemical fertilizer (F3A3) significantly increased the yield of saline-alkali rice, which was 6.62% higher than that of the F0A1 treatment, thereby validating the meta-analysis outcomes. We propose that the combined use of humic acid organic fertilizer and chemical fertilizer can promote the growth of rice in saline-alkali soils. Consequently, these management practices provide a means to foster the green and healthy development of rice in saline-alkali regions across China.
{"title":"A Meta-Analysis of 30 Years in China and Micro-District Experiments Shows Organic Fertilizer Quantification Combined with Chemical Fertilizer Reduction Enhances Rice Yield on Saline-Alkali Land","authors":"He Chen , Ruan Yunze , Jia Zhongjun","doi":"10.1016/j.rsci.2025.01.004","DOIUrl":"10.1016/j.rsci.2025.01.004","url":null,"abstract":"<div><div>To improve the yield and quality of rice grown on saline-alkali soil, a meta-analysis combined with micro-district experimental studies was conducted in China to examine the impact of humic acid- based organic fertilizer and chemical fertilizer on rice yield and quality. This study employed a two-factor fully randomized experimental design, incorporating four levels of humic acid (F0, 0.0 g/pot; F1, 4.8 g/pot; F2, 12.0 g/pot; and F3, 19.2 g/pot) and three levels of chemical fertilizer (A1, full conventional dosage; A2, 85% of conventional dosage; and A3, 70% of conventional dosage). The meta-analysis revealed that the application of organic fertilizer (at a rate of 1500‒3000 kg/hm<sup>2</sup>) combined with chemical fertilizer had a significantly positive effect on the theoretical yield, tiller number, partial factor productivity, and SPAD value of rice. Temperature, organic fertilizer application, and chemical fertilizer levels were identified as critical factors affecting rice yield. The micro-experiments demonstrated that the application of humic acid organic fertilizer with treatment F3 significantly elevated the SPAD value at the full heading and grain filling stages. Increased panicle number and seed-setting rate were the main contributors to the rise in yield, with the F3 treatment yielding the highest overall. The effective leaf area, high-efficiency leaf area, and dry matter accumulation in rice treated with F3 were all higher compared with the F0 treatment. Our findings indicated that the addition of humic acid organic fertilizer can markedly improve the partial factor productivity and agronomic efficiency of rice. In conclusion, the application of F3 organic fertilizer combined with A3 chemical fertilizer (F3A3) significantly increased the yield of saline-alkali rice, which was 6.62% higher than that of the F0A1 treatment, thereby validating the meta-analysis outcomes. We propose that the combined use of humic acid organic fertilizer and chemical fertilizer can promote the growth of rice in saline-alkali soils. Consequently, these management practices provide a means to foster the green and healthy development of rice in saline-alkali regions across China.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 259-272"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.rsci.2024.11.002
He Zhenrui , Zhao Wenhua , Cheng Baoping , Yang Mei , Yang Yingqing , Zhu Yiming , Zhou Erxun
Rice false smut (RFS), caused by the ascomycete fungus Ustilaginoidea virens (Cooke) Takahashi, is a notable panicle disease of rice. Mycoviruses represent a category of viruses capable of infecting various fungal groups, garnering interest for their potential application in the biological control of plant fungal diseases. We identified a novel positive-sense single-stranded RNA virus, named Ustilaginoidea virens mitovirus 1 (UvMV1), from an abnormal U. virens strain Uv488. The complete genome sequence of UvMV1 is 2 474 nt in length and contains a large open reading frame encoding RNA-dependent RNA polymerase. UvMV1 is classified as a new member of the genus Unuamitovirus in the family Mitoviridae based on phylogenetic analysis. It is effectively transmitted vertically through conidia of strain Uv488 and horizontally through hyphal fusion between vegetatively compatible individuals with an 80% transmission efficiency. We further demonstrated that UvMV1 significantly influenced conidiation, colony morphology, growth rate, secondary metabolite production, and the response to environmental stress in U. virens. Furthermore, qRT-PCR results aimed at detecting the expression levels of autophagy-related genes suggested that UvMV1 infection had the potential to induce autophagy in U. virens. Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in U. virens, while inhibition of autophagy results in decreased UvMV1 titers. Transcriptome analyses consistently demonstrated that UvMV1 regulated the expression of genes associated with secondary metabolism, mycelial growth, virulence, and the mitogen-activated protein kinase signaling pathway, thereby influencing both the antiviral response and the virulence of U. virens. The data presented collectively identified a novel mycovirus responsible for inducing growth abnormalities in U. virens, and elucidated the regulatory mechanisms during mycovirus-host fungus interactions, particularly the autophagy pathway. These findings offer new perspectives and potential control strategies for managing RFS in agricultural settings.
{"title":"Molecular and Biological Characterization of Novel Mitovirus Infecting Phytopathogenic Fungus Ustilaginoidea virens","authors":"He Zhenrui , Zhao Wenhua , Cheng Baoping , Yang Mei , Yang Yingqing , Zhu Yiming , Zhou Erxun","doi":"10.1016/j.rsci.2024.11.002","DOIUrl":"10.1016/j.rsci.2024.11.002","url":null,"abstract":"<div><div>Rice false smut (RFS), caused by the ascomycete fungus <em>Ustilaginoidea virens</em> (Cooke) Takahashi, is a notable panicle disease of rice. Mycoviruses represent a category of viruses capable of infecting various fungal groups, garnering interest for their potential application in the biological control of plant fungal diseases. We identified a novel positive-sense single-stranded RNA virus, named <em>Ustilaginoidea virens</em> mitovirus 1 (UvMV1), from an abnormal <em>U. virens</em> strain Uv488. The complete genome sequence of UvMV1 is 2 474 nt in length and contains a large open reading frame encoding RNA-dependent RNA polymerase. UvMV1 is classified as a new member of the genus <em>Unuamitovirus</em> in the family <em>Mitoviridae</em> based on phylogenetic analysis. It is effectively transmitted vertically through conidia of strain Uv488 and horizontally through hyphal fusion between vegetatively compatible individuals with an 80% transmission efficiency. We further demonstrated that UvMV1 significantly influenced conidiation, colony morphology, growth rate, secondary metabolite production, and the response to environmental stress in <em>U. virens</em>. Furthermore, qRT-PCR results aimed at detecting the expression levels of autophagy-related genes suggested that UvMV1 infection had the potential to induce autophagy in <em>U. virens</em>. Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in <em>U. virens</em>, while inhibition of autophagy results in decreased UvMV1 titers. Transcriptome analyses consistently demonstrated that UvMV1 regulated the expression of genes associated with secondary metabolism, mycelial growth, virulence, and the mitogen-activated protein kinase signaling pathway, thereby influencing both the antiviral response and the virulence of <em>U. virens</em>. The data presented collectively identified a novel mycovirus responsible for inducing growth abnormalities in <em>U. virens</em>, and elucidated the regulatory mechanisms during mycovirus-host fungus interactions, particularly the autophagy pathway. These findings offer new perspectives and potential control strategies for managing RFS in agricultural settings.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 243-258"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.rsci.2024.12.005
Fang Pengpeng , Sun Pingyong , Zhang Li , Wang Pengfei , Zeng Jia , He Qiang , Shu Fu , Xing Yongzhong , Deng Huafeng , Zhang Wuhan
{"title":"Mapping of Red Hull Gene OsRH3 and Its Great Value in Mechanized Hybrid Rice Seed Production","authors":"Fang Pengpeng , Sun Pingyong , Zhang Li , Wang Pengfei , Zeng Jia , He Qiang , Shu Fu , Xing Yongzhong , Deng Huafeng , Zhang Wuhan","doi":"10.1016/j.rsci.2024.12.005","DOIUrl":"10.1016/j.rsci.2024.12.005","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 147-151"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.rsci.2024.12.001
Jiajun Liu , Cuili Wang , Mingmao Sun , Xiaoding Ma , Bing Han , Xiaohong Guo , Longzhi Han , Di Cui
{"title":"Genome-Wide Discovery of Candidate Genes Associated with Cold Tolerance in Rice at Various Growth Stages","authors":"Jiajun Liu , Cuili Wang , Mingmao Sun , Xiaoding Ma , Bing Han , Xiaohong Guo , Longzhi Han , Di Cui","doi":"10.1016/j.rsci.2024.12.001","DOIUrl":"10.1016/j.rsci.2024.12.001","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 137-142"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-01DOI: 10.1016/j.rsci.2024.09.002
Wang Jingqing , Wang Yaliang , Chen Yulin, Chen Huizhe, Xiang Jing, Zhang Yikai, Wang Zhigang, Zhang Yuping
Rice yield is heavily reliant on the number of spikelets per panicle, a factor determined by the processes of spikelet differentiation and degeneration. In rice cultivars with large panicles, spikelet degeneration negates the advantages of large panicle and constrains yield potential. Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration, with the sensitive period for this process commencing approximately 15‒20 d before panicle heading. Notable positional variations occur within the panicle, with significantly higher spikelet degeneration rates at the basal than at the upper positions. An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress. Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport, which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation. Sucrose serves as the main carbohydrate source for spikelet development, demonstrating an apical dominance pattern that favors spikelet formation. However, under abiotic stress, the inhibition of sucrose decomposition, rather than sucrose transport impairment, predominantly contributes to aggravated spikelet degeneration at the basal panicle positions. Brassinolide and auxin have a significant relationship with spikelet formation, potentially mediating apical dominance. Specifically, brassinolide enhances sucrose accumulation and utilization, thereby alleviating spikelet degeneration. At present, the mechanisms underlying rice spikelet degeneration have not been fully revealed, and the joint effects of hormones, carbohydrates, and carbon and nitrogen metabolism on this process require further investigation. To reduce the spikelet degeneration, the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’ resilience to abiotic stress. An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.
水稻产量在很大程度上取决于每个圆锥花序的小穗数，而小穗数是由小穗分化和退化过程决定的。在大圆锥花序的水稻栽培品种中，小穗退化抵消了大圆锥花序的优势，限制了产量潜力。环境胁迫引起的植物新陈代谢紊乱会加剧小穗退化，这一过程的敏感期大约在圆锥花序抽穗前 15-20 d 开始。圆锥花序内的位置差异显著，基部位置的小穗退化率明显高于上部位置。碳氮代谢失衡是非生物胁迫下小穗退化加剧的主要生理基础。碳氮代谢失调会导致能量代谢紊乱和呼吸电子传递失调，从而通过过量的活性氧积累加速幼穗凋亡。蔗糖是小穗发育的主要碳水化合物来源，其顶端优势模式有利于小穗的形成。然而，在非生物胁迫下，抑制蔗糖分解，而不是蔗糖运输障碍，是导致基部圆锥花序位置小穗退化加剧的主要原因。芸苔素内酯和辅助素与小穗的形成有重要关系，可能是顶端优势的介导因素。具体来说，芸苔素内酯能促进蔗糖的积累和利用，从而缓解小穗退化。目前，水稻小穗退化的机理尚未完全揭示，激素、碳水化合物和碳氮代谢对这一过程的共同影响还需要进一步研究。为减少小穗退化，有策略地施用水肥以建立稳定的水稻群体，可增强水稻植株对非生物性胁迫的抗逆性。减少小穗退化的有效方法是在圆锥花序萌发期增加每个小穗的干物质占有量。
{"title":"Progress on Physiological Mechanisms of Rice Spikelet Degeneration at Different Panicle Positions Caused by Abiotic Stress","authors":"Wang Jingqing , Wang Yaliang , Chen Yulin, Chen Huizhe, Xiang Jing, Zhang Yikai, Wang Zhigang, Zhang Yuping","doi":"10.1016/j.rsci.2024.09.002","DOIUrl":"10.1016/j.rsci.2024.09.002","url":null,"abstract":"<div><div>Rice yield is heavily reliant on the number of spikelets per panicle, a factor determined by the processes of spikelet differentiation and degeneration. In rice cultivars with large panicles, spikelet degeneration negates the advantages of large panicle and constrains yield potential. Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration, with the sensitive period for this process commencing approximately 15‒20 d before panicle heading. Notable positional variations occur within the panicle, with significantly higher spikelet degeneration rates at the basal than at the upper positions. An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress. Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport, which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation. Sucrose serves as the main carbohydrate source for spikelet development, demonstrating an apical dominance pattern that favors spikelet formation. However, under abiotic stress, the inhibition of sucrose decomposition, rather than sucrose transport impairment, predominantly contributes to aggravated spikelet degeneration at the basal panicle positions. Brassinolide and auxin have a significant relationship with spikelet formation, potentially mediating apical dominance. Specifically, brassinolide enhances sucrose accumulation and utilization, thereby alleviating spikelet degeneration. At present, the mechanisms underlying rice spikelet degeneration have not been fully revealed, and the joint effects of hormones, carbohydrates, and carbon and nitrogen metabolism on this process require further investigation. To reduce the spikelet degeneration, the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’ resilience to abiotic stress. An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 193-202"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although metabolic homeostasis disruption, cellular damage, and premature senescence caused by salinity stress are well-documented in the literature, there are few studies investigating cytological changes induced by salinity stress within the altered metabolic landscape of rice, and this study aims to fill that gap. The cytological characterization of root tips (in terms of mitotic index and chromosomal abnormalities such as stickiness, laggards, fragments, bridges, micronuclei, ring chromosomes, and total mitotic abnormalities) was conducted on 10 experimental rice landraces from coastal Bangladesh, grown under post-imbibitional salinity stress (PISS), while correlating these changes with their metabolic status. The results revealed a strong correlation between salinity-induced cytological changes in root cells (mitotic index and chromosomal abnormalities) and the redox interactome status of all experimental rice landraces. The landraces Kutepatnai, Talmugur, Nonakochi, and Benapol, which exhibited a higher ability to mitigate PISS-induced chromosomal abnormalities and improve mitotic index, also showed lower accumulation of oxidative stress markers (protein carbonylation, lipid peroxidation, prooxidant accumulation, oxidative stress index, reactive oxygen species (ROS)-antioxidative stress index, and efficiency of ROS processing via the Halliwell-Asada pathway) compared with more susceptible landraces (Charobalam, Jotaibalam, Kachra, and Lalmota). These findings underscore the role of redox biology in preventing chromotoxic effects under salinity stress. Hierarchical cluster analysis and principal component analysis, used to determine variations and similarities among the experimental rice landraces based on cytological attributes, redox interactome, and physiological phenotypes, classified the landraces according to their salinity tolerance and sensitivity. This study proposes a novel approach for exploring redox-regulated cytological fingerprints as a tool for identifying salinity-tolerant rice landraces.
{"title":"A Novel Approach for Screening Salinity-Tolerant Rice Germplasm by Exploring Redox-Regulated Cytological Fingerprint","authors":"Uthpal Krishna Roy, Babita Pal, Soumen Bhattacharjee","doi":"10.1016/j.rsci.2025.03.006","DOIUrl":"10.1016/j.rsci.2025.03.006","url":null,"abstract":"<div><div>Although metabolic homeostasis disruption, cellular damage, and premature senescence caused by salinity stress are well-documented in the literature, there are few studies investigating cytological changes induced by salinity stress within the altered metabolic landscape of rice, and this study aims to fill that gap. The cytological characterization of root tips (in terms of mitotic index and chromosomal abnormalities such as stickiness, laggards, fragments, bridges, micronuclei, ring chromosomes, and total mitotic abnormalities) was conducted on 10 experimental rice landraces from coastal Bangladesh, grown under post-imbibitional salinity stress (PISS), while correlating these changes with their metabolic status. The results revealed a strong correlation between salinity-induced cytological changes in root cells (mitotic index and chromosomal abnormalities) and the redox interactome status of all experimental rice landraces. The landraces Kutepatnai, Talmugur, Nonakochi, and Benapol, which exhibited a higher ability to mitigate PISS-induced chromosomal abnormalities and improve mitotic index, also showed lower accumulation of oxidative stress markers (protein carbonylation, lipid peroxidation, prooxidant accumulation, oxidative stress index, reactive oxygen species (ROS)-antioxidative stress index, and efficiency of ROS processing via the Halliwell-Asada pathway) compared with more susceptible landraces (Charobalam, Jotaibalam, Kachra, and Lalmota). These findings underscore the role of redox biology in preventing chromotoxic effects under salinity stress. Hierarchical cluster analysis and principal component analysis, used to determine variations and similarities among the experimental rice landraces based on cytological attributes, redox interactome, and physiological phenotypes, classified the landraces according to their salinity tolerance and sensitivity. This study proposes a novel approach for exploring redox-regulated cytological fingerprints as a tool for identifying salinity-tolerant rice landraces.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 228-242"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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