Journal of Herbal Medicine最新文献_第6页

Therapeutic potential of wild rare plant-derived herbal ointment: a promising intervention for managing chronic dermatological conditions 野生珍稀植物草药软膏的治疗潜力：治疗慢性皮肤病的有效干预措施

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-23 DOI: 10.1016/j.hermed.2024.100920

Mohamad Al Dhareef Al Shamsi

Introduction

Chronic skin conditions have become a significant global healthcare issue, necessitating the development of novel therapeutic approaches. This study investigates the medicinal properties of ABBA Herbal Ointment, derived from the wild plant Cymbopogon schoenanthus ssp. proximus. Introduced in 2001, this ointment contains natural ingredients such as Aloe vera, jojoba oil, and calendula, extracted from desert medicinal plants. It has demonstrated swift and conclusive efficacy in treating chronic skin conditions. Clinically verified and patented in Gulf Cooperation Council (GCC) countries (Patent Number: GC 0001218), its therapeutic potential is supported by extensive research conducted by three French laboratories.

Methods

The traditional method is used to produce the ointment from the plant extract. To make the ointment, the extracted plant chlorophyll was homogenised with olive oil and natural beeswax. To further validate the therapeutic potential of the ointment, in vitro occlusive tests were performed to assess its efficacy on human skin, with results observed after 48 hours. In this study, 24 volunteers participated, of which two discontinued.

Results

The topical application of the ointment shows no side effects, and further trials demonstrate its beneficial effects in managing chronic skin diseases.

Conclusion

ABBA Herbal Ointment, derived from the rare wild plant C. schoenanthus ssp. proximus, shows significant potential as an effective treatment for chronic dermatological conditions. The study supports the ointment's efficacy and safety, aligning with the goal of developing novel therapeutic approaches for chronic skin diseases.

导言：慢性皮肤病已成为一个重要的全球医疗保健问题，因此有必要开发新的治疗方法。本研究调查了 ABBA 草本软膏的药用特性，该软膏提取自野生植物 Cymbopogon schoenanthus ssp.proximus。这种软膏于 2001 年推出，含有从沙漠药用植物中提取的芦荟、荷荷巴油和金盏花等天然成分。它在治疗慢性皮肤病方面具有迅速和确凿的疗效。经临床验证，该药膏在海湾合作委员会（GCC）国家获得了专利（专利号：GC 0001218），其治疗潜力得到了法国三家实验室广泛研究的支持。制作软膏时，将提取的植物叶绿素与橄榄油和天然蜂蜡混合均匀。为了进一步验证药膏的治疗潜力，还进行了体外闭塞试验，以评估其对人体皮肤的疗效，48 小时后观察结果。这项研究共有 24 名志愿者参加，其中两人中断了研究。结果软膏的局部使用没有显示出副作用，进一步的试验证明了它在治疗慢性皮肤病方面的有益效果。这项研究支持药膏的有效性和安全性，符合开发新型慢性皮肤病治疗方法的目标。

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引用次数: 0

The inheritance and development of traditional Chinese medicine formulas in treating childhood asthma based on the perspective of clinical research 基于临床研究视角的中药方剂治疗小儿哮喘的继承与发展

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-23 DOI: 10.1016/j.hermed.2024.100921

Fangyang Guo , Yonghuan Xiao , Chaitanya N. Makwana , Yuan He , Pan Luo , Muhammad Irfan , Zhiwen Xiao , Ling Li , Meiying Ao , Qian Liu

Introduction

The inheritance and development status are illustrated with research hotspots, medication patterns, and research quality based on the two literature databases of previous and recent clinical investigations of Traditional Chinese Medicine formulas in treating childhood asthma.

Methods

The relevant literature was retrieved from seven databases. Based on the year of publication, dividing the body of literature produced since the database's founding into previous and recent databases. Bibliometric, VOSviewer, IBM SPSS Modeller and modified Jadad scale were used to conduct characteristic analysis, visual analysis, herb compatibility rule analysis and research quality evaluation respectively.

Results

One hundred and seven previous studies and 212 recent studies were included. The outcome indicators are inherited symptoms and syndrome, and develop into mechanistic indicators such as pulmonary function. The common herbs are Glycyrrhiza uralensis Fisch., Ephedra sinica Stapf and Prunus armeniaca L.var.ansu Maxim., and the development of Schisandra chinensis (Turcz.) Baill., Astragalus membranaceus (Fisch.) Bge.var. mongholicus (Bge.) Hsiao and Poria cocos (Schw.) Wolf are increasing. The compatibility of herbs inherited the combination of E. sinica Stapf and G. uralensis Fisch. based on the development of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag., S. chinensis (Turcz.) Baill. and Pinellia ternata (Thunb.) Breit. The research quality has been improved.

Conclusion

From the clinical research perspective, the study of Traditional Chinese Medicine formulas for the treatment of childhood asthma has its own unique inheritace and development, covering a number of areas.

引言基于中医方剂治疗小儿哮喘的既往和近期临床研究两个文献数据库，从研究热点、用药规律、研究质量等方面阐述了中医方剂治疗小儿哮喘的继承与发展现状。根据发表年份，将数据库建立以来的文献分为以前和最近的数据库。采用文献计量学、VOSviewer、IBM SPSS Modeller 和修正的 Jadad 量表分别进行特征分析、可视化分析、草本相容性规则分析和研究质量评价。结果指标由遗传症状和综合征发展为肺功能等机理指标。常见的中草药有甘草、麻黄、杨梅，五味子、黄芪、白术、茯苓等中草药的应用也在不断增加。在发展 Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag.、S. chinensis (Turcz.) Baill.和 Pinellia ternata (Thunb.) Breit.结论从临床研究的角度来看，中药方剂治疗小儿哮喘的研究有其独特的继承性和发展性，涉及多个领域。

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引用次数: 0

Estrogenic analysis of ten Thai medicinal plants used for treating menstrual irregularity by ERα and ERβ yeast two-hybrid systems 利用ERα和ERβ酵母双杂交系统分析治疗月经不调的十种泰国药用植物的雌激素作用

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-23 DOI: 10.1016/j.hermed.2024.100922

Palita Paewthaisong, Ratima Janthima, Sineenat Siri

Introduction

Several traditional Thai medicinal plants have historically been utilised to alleviate menstrual irregularities. While it is hypothesised that phytoestrogens are pivotal in their medicinal efficacy, more empirical evidence is needed to verify this assumption. Furthermore, our understanding of the estrogenic effects mediated by estrogen receptors (ERα and ERβ) and their alterations post-intestinal enzymatic digestion is limited. This study aims to assess the estrogenic activity of 10 medicinal plants before and after in vitro simulated gastrointestinal digestion using yeast two-hybrid (Y2H) systems.

Methods

Y2H systems were constructed based on the interaction between the ligand-binding domain of estrogen receptors and the receptor interaction domain of coactivators. The medicinal plants were extracted in water at 95 °C for 1 hour or in 70% ethanol for 24 hours (1: 8 w/v). Their estrogenic activities before and after in vitro gastrointestinal digestion were determined using Y2H systems.

Results

The majority of ethanol extracts exhibited higher estrogenic activity compared to water extracts, with detection notably facilitated by the ERβ-Y2H system. This study represents the first documentation of the estrogenic activities of Dendrolobium lanceolatum and Morinda coreia, both prominently identified by the ERβ-Y2H system. Specifically, the D. lanceolatum extract demonstrated the highest estrogenic activity, showing a significant enhancement following gastrointestinal enzymatic digestion, suggesting the presence of active metabolites.

Conclusions

These findings advance our understanding of the estrogenic properties of medicinal plants for treating menstrual irregularities, clarifying their effects on estrogen receptor subtypes and post-hydrolysis activities. Such insights are vital for determining their potential as alternative medicines.

导言：泰国的几种传统药用植物历来被用来缓解月经不调。虽然有人假设植物雌激素在其药用功效中起着关键作用，但还需要更多的经验证据来验证这一假设。此外，我们对雌激素受体（ERα和ERβ）介导的雌激素效应及其在肠道酶解后的变化了解有限。本研究旨在利用酵母双杂交（Y2H）系统评估10种药用植物在体外模拟胃肠道消化前后的雌激素活性。药用植物在 95 °C 的水中提取 1 小时或在 70% 的乙醇中提取 24 小时（1:8 w/v）。结果与水提取物相比，大多数乙醇提取物表现出更高的雌激素活性，ERβ-Y2H 系统的检测效果显著。本研究首次记录了Dendrobium lanceolatum和Morinda coreia的雌激素活性，这两种植物都是通过ERβ-Y2H系统鉴定的。具体来说，D. lanceolatum 提取物的雌激素活性最高，在胃肠道酶解后活性显著增强，表明存在活性代谢产物。这些见解对于确定它们作为替代药物的潜力至关重要。

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引用次数: 0

The use of herbal medicine for hypertension in rural and urban Thailand: a cross sectional study 泰国城乡居民使用草药治疗高血压的情况：横断面研究

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-17 DOI: 10.1016/j.hermed.2024.100916

Nutchar Wiwatkunupakarn , Chanchanok Aramrat , Pimtip Sanguanwai , Yanee Choksomngam , Mary E. Gilder , Wichuda Jiraporncharoen , Rose McGready , Chaisiri Angkurawaranon

Introduction

Using herbal medicine to treat hypertension is common in Thailand. However, there is variation in the use of herbs between rural and urban areas. This study aims to observe the prevalence of herb use, describe herb users’ behaviours, and describe anti-hypertensive drug compliance among the users.

Methods

A cross-sectional survey among hypertensive patients visiting primary care services in Tak (rural) and Chiang Mai (urban) provinces was conducted between March and April 2019. Patients were assessed by using questionnaires that gathered information about patient demographic data, history of hypertension, herb use behaviours, and the concurrent use of standard treatment.

Results

In total, 400 participants were included in this study—200 from rural and 200 from urban areas. Sixty-two percent were female. The prevalence of herb use was 13.0% in urban areas and 17.5% in the rural areas. Rural participants reported a significantly longer duration of herb use (P < 0.01), and a significantly higher proportion of using unapproved herbal products (91% vs 54%, P < 0.001). Participants mostly use herbs along with standard treatment (86.9%); nevertheless, poor drug compliance was similar in both areas, with rural participants more frequently taking their medications at the wrong time (P = 0.03).

Conclusion

There was a trend of higher use of unapproved herbals in rural participants. Drug compliance among herb users was poor. It is recommended that a comprehensive assessment of herb use is conducted during care for hypertensive patients in this and similar contexts.

导言使用草药治疗高血压在泰国很常见。然而，农村和城市地区在使用草药方面存在差异。本研究旨在观察草药使用的普遍程度，描述草药使用者的行为，并说明使用者服用抗高血压药物的依从性。方法 2019年3月至4月期间，在德府（农村）和清迈府（城市）的基层医疗服务机构对高血压患者进行了横断面调查。调查问卷收集了患者的人口统计学数据、高血压病史、草药使用行为以及同时使用标准治疗的情况。女性占 62%。城市地区使用草药的比例为 13.0%，农村地区为 17.5%。农村参与者使用草药的时间明显更长（P <0.01），使用未经批准的草药产品的比例明显更高（91% 对 54%，P <0.001）。参与者大多在接受标准治疗的同时使用草药（86.9%）；然而，两个地区的服药依从性都很差，农村参与者更常在错误的时间服药（P = 0.03）。草药使用者的服药依从性很差。建议在该地区及类似地区对高血压患者进行护理时对草药使用情况进行全面评估。

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引用次数: 0

A review on ethnomedicine, phytochemistry, pharmacology, and toxicology of Pedalium murex L. "Pedalium murex L 的民族医药学、植物化学、药理学和毒理学综述"。

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-17 DOI: 10.1016/j.hermed.2024.100917

Amrender Singh Rao, Surender Singh Yadav, Neetu Singh, Abhishek Nandal

Introduction

Pedalium murex L. (Gokhru) is a succulent herbaceous plant with multifarious medicinal values in various traditional therapeutic systems. The review summarises the previous research findings on P. murex, with major emphasis on ethnomedicine, phytochemistry, pharmacology, and toxicology.

Material and methods

The study was performed by retrieving relevant literature from scientific databases like Scopus, Science Direct, Springer Link, Wiley, PubMed, Google Scholar, Elsevier, and Web of Science. The data collection was carried out using specific keywords like ‘P. murex,’ ‘Gokhru,’ ‘ethnomedicinal uses,’ ‘Phytochemistry,’ ‘Pharmacology,’ ‘Toxicity,’ ‘Clinical studies’ and their combinations.

Results

The study's findings revealed a strong connection between P. murex traditional use and pharmacological activities. The plant shows aphrodisiac, antiulcer, anti-inflammatory, antioxidant, antimicrobial, antidiabetic, antivenereal, anticancer, analgesic, antipyretic, immune-modulator, and other pharmacological properties. Phytocompounds like diosgenin, pedalin, pedalitin, β-sitosterol, isatin, lupeol acetate, and ursolic acid are amongst the many which are responsible for various therapeutic applications and have the potential to transform into oral drugs.The plant shows immune-modulatory effects by influencing corticosterone, low-density lipoprotein, thiobarbituric acid, lipid peroxidation, nitric oxide, exo-polysaccharides, Cyclooxygenase and free radical inhibitory mechanisms for neuroprotection, hypolipidemic, antidiabetic, antibiofilm, aphrodisiac, and anti-inflammatory activities respectively. Toxicological studies implied safety and protection indicating potential to be used as herbal medicine.

Conclusion

The different parts of the plant have potential bioactive properties with prospective ethnopharmacological relevance for aphrodisiac, venereal, urinary tract infections and inflammation. Further, it is necessary to conduct controlled clinical trials, in-depth pharmacokinetic, detailed phytochemical and pharmacological studies to achieve the plant’s full potential.

引言 Pedalium murex L.（Gokhru）是一种肉质草本植物，在各种传统治疗系统中具有多种药用价值。本综述总结了以前关于 P. murex 的研究成果，主要侧重于民族医药学、植物化学、药理学和毒理学。材料与方法本研究通过检索 Scopus、Science Direct、Springer Link、Wiley、PubMed、Google Scholar、Elsevier 和 Web of Science 等科学数据库中的相关文献进行。数据收集使用了特定的关键词，如 "P. murex"、"Gokhru"、"民族药用"、"植物化学"、"药理学"、"毒性"、"临床研究 "及其组合。该植物具有壮阳、抗溃疡、抗炎、抗氧化、抗菌、抗糖尿病、抗蛇毒、抗癌、镇痛、解热、免疫调节等药理特性。许多植物化合物，如 diosgenin、pedalin、pedalitin、β-谷甾醇、isatin、lupeol acetate 和熊果酸等，都具有多种治疗作用，并有可能转化为口服药物。该植物通过影响皮质酮、低密度脂蛋白、硫代巴比妥酸、脂质过氧化、一氧化氮、外多糖、环氧化酶和自由基抑制机制，显示出免疫调节作用，分别具有神经保护、降血脂、抗糖尿病、抗生物膜、壮阳和抗炎活性。毒理学研究表明，该植物的不同部分具有潜在的生物活性，在壮阳、性病、尿路感染和炎症方面具有民族药理学意义。此外，有必要进行对照临床试验、深入的药代动力学、详细的植物化学和药理学研究，以充分发挥该植物的潜力。

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引用次数: 0

Optimisation and Evaluation of the Correlation of Extraction Conditions for Total Flavonoid Content and Antixanthine Oxidase Activity in Psidium guajava 优化和评估萃取条件对番石榴总黄酮含量和抗黄嘌呤氧化酶活性的相关性

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-14 DOI: 10.1016/j.hermed.2024.100915

T.M. Le , C.D.P. Nguyen , A.C. Ha

Introduction

Psidium guajava L. (P guajava) and its leaf, one of the most popular plants in traditional medicine and cuisine, were demonstrated to have high flavonoid content with many bioactivities, including antigout activity. However, the correlation between total flavonoid content (TFC) and antigout activities and the optimal conditions for extracting P guajava have not been reported.

Methods

This study established the optimisation of ethanol extracts of P guajava leaves for TFC and antigout activities using the single-factor method and response surface methodology. The antigout activities were determined through the xanthine oxidase inhibition (XOI) method.

Results

The four independent variables (ethanol concentration, plant-solvent ratio, extraction time, and temperature) significantly impacted two response variables (TFC and XOI activity). The optimal extracts for XOI activity reach the maximum TFC of 323.86 ± 2.26 mg quercetin/g and XOI activity of 26.37 ± 0.5 μg/ml. The changes in the chemical structure of extracts were analysed by Fourier transform infrared, which indicated that several flavonoids in P guajava do not have XOI activity. The correlation of TFC and XOI is robust with the Pearson correlation of −0.882 and Spearman Rho test of −0.884. Additionally, the biological activities, such as antioxidant and anti-inflammatory, of optimal extracts for XOI and TFC were determined to extend the application capacity to support the treatment of gout diseases.

Conclusions

The optimised extraction conditions are critical to isolating flavonoids, which can serve as functional food or medicine for managing gout and its complications.

引言番石榴（Psidium guajava L.）及其叶片是传统医学和烹饪中最受欢迎的植物之一，其黄酮类化合物含量高，具有多种生物活性，包括抗痛风活性。本研究采用单因素法和响应面法对番石榴叶乙醇提取物的总黄酮含量和抗痛风活性进行了优化。结果四个自变量（乙醇浓度、植物-溶剂比率、提取时间和温度）对两个响应变量（TFC 和 XOI 活性）有显著影响。XOI 活性的最佳提取物达到了最大 TFC（323.86 ± 2.26 毫克槲皮素/克）和 XOI 活性（26.37 ± 0.5 微克/毫升）。通过傅立叶变换红外光谱分析了提取物化学结构的变化，结果表明番石榴中的几种黄酮类化合物不具有 XOI 活性。TFC 与 XOI 的相关性很强，Pearson 相关性为-0.882，Spearman Rho 检验为-0.884。此外，还确定了 XOI 和 TFC 最佳提取物的抗氧化和抗炎等生物活性，以扩大其应用能力，支持痛风疾病的治疗。

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引用次数: 0

Corrigendum to “Effect of Lantana camara ethanolic leaf extract on survival and migration of MDA-MB-231 triple-negative breast cancer cell line” [Journal of Herbal Medicine 43 (2024) 100837] 香蒲乙醇叶提取物对 MDA-MB-231 三阴性乳腺癌细胞系存活和迁移的影响》[《中草药杂志》43 (2024) 100837]更正

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-13 DOI: 10.1016/j.hermed.2024.100910

Arundhaty Pal, Sourav Sanyal, Sayantani Das, Tapas K. Sengupta

引用次数: 0

Can herbal teas be used as complementary therapies for kidney disorders? Results from a cross-sectional study 草药茶可以作为肾脏疾病的辅助疗法吗？横断面研究结果

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-09 DOI: 10.1016/j.hermed.2024.100914

Firouzeh Moeinzadeh , Mohammad Hossein Rouhani , Mojgan Mortazavi , Farnaz Shahdadian

Introduction

Chronic kidney disease (CKD) has limited treatment options and is becoming more and more common. Since apparent evidence in the literature shows that herbs may be reno-protective, we discussed different kinds of herbal tea in relation to CKD and related disorders.

Methods

This cross-sectional study was conducted in health care centres located in a region in central Iran by enroling subjects aged 18 years and older. A self-reported questionnaire was used to evaluate the frequency and type of herbal tea drinks by participants. Anthropometric characteristics were measured and blood samples were obtained using a standard procedure. The Kidney Disease Outcome Quality Initiative (K/DOQI) guideline was the reference for diagnosis of CKD. Through multiple logistic models, the association between herbal tea drinking with CKD and related disorders was examined.

Results

Overall, 3 322 subjects were examined in this study. Three types of herbal tea were consumed by the study participants including green tea, chamomile-thyme tea, and borage tea. No statistically significant consistent associations between kidney disorders, including CKD, haematuria, albuminuria, and kidney stone and consumption of these herbal preparations were identified.

Conclusion

Overall, this study failed to suggest any consistent association between renal function and three categories of herbal tea in adult Iranian individuals.

引言慢性肾脏病（CKD）的治疗方法有限，而且越来越常见。由于文献中有明显证据表明草药可能具有肾脏保护作用，因此我们讨论了不同种类的草药茶与 CKD 及相关疾病的关系。研究采用自我报告问卷调查法评估受试者饮用凉茶的频率和类型。采用标准程序测量了人体测量特征并采集了血液样本。肾脏疾病结果质量倡议（K/DOQI）指南是诊断慢性肾脏病的参考标准。通过多重逻辑模型，研究了饮用凉茶与 CKD 及相关疾病之间的关系。研究对象饮用了三种花草茶，包括绿茶、洋甘菊-百里香茶和琉璃苣茶。结论总体而言，这项研究未能表明伊朗成年人的肾功能与三类草药茶之间存在任何一致的联系。

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引用次数: 0

Comparative Study on the Effects of Myrtle and Chamomile Essential Oils on Pain and Episiotomy Wound Healing in Primiparous Women: A Randomised Controlled Clinical 桃金娘精油和洋甘菊精油对初产妇疼痛和外阴切开术伤口愈合影响的比较研究：随机对照临床研究

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-07-04 DOI: 10.1016/j.hermed.2024.100913

S. Moradkhani , S. Abdoli , E.T. Ghane , E. Jenabi

Introduction

Episiotomy is a common surgical procedure that affects the quality of life of women. Chamomile and myrtle have been used to treat wounds since antiquity. This study aimed to compare the effects of topical formulations containing essential oils (EOs) of chamomile, myrtle, , or a placebo on pain intensity and episiotomy wound healing in primiparous women.

Methods

This randomised and controlled clinical trial was conducted in the city of Hamadan in 2020. To this end, 120 parturient mothers were randomly assigned to one of three groups. They applied topical formulations containing 1% EO of chamomile, myrtle, or placebo twice daily for 10 days. Data collection was subsequently performed by assessing demographic, gynaecological, possible signs of side effects, pain intensity, and the wound-healing process 6 hours after surgery, days fifth and tenth after the intervention. In addition, the Visual Analogue Scale and Redness, Oedema, Ecchymosis, Discharge, and Approximation were utilised to examine pain intensity and healing processes. R software (v. 4.0.5) was employed for data analysis. The statistical significance level was P < 0.05.

Results

There was no significant difference between groups at baseline. Furthermore, the mean pain intensity score in the three groups indicated no statistically significant differences. The group that received chamomile experienced the best wound-healing process (P < 0.05) on the 10th day. Phytochemical investigations revealed the presence of α-bisabolol oxide A, α-bisabolol oxide B, β-farnesene, and chamazulene in chamomile EO, and α-pinene, 1,8-cineol, linalool, α-terpineol, linalool acetate, and limonene in myrtle EO.

Conclusions

A chamomile-containing formulation may promote episiotomy healing.

导言开腹手术是一种影响妇女生活质量的常见外科手术。洋甘菊和桃金娘自古以来就被用来治疗伤口。本研究旨在比较含有洋甘菊、桃金娘精油（EO）或安慰剂的外用配方对初产妇疼痛强度和外阴切开术伤口愈合的影响。方法这项随机对照临床试验于 2020 年在哈马丹市进行。为此，120 名产妇被随机分配到三组中的一组。她们使用含 1%洋甘菊、桃金娘或安慰剂的外用制剂，每天两次，持续 10 天。随后通过评估人口统计学、妇科、可能出现的副作用迹象、疼痛强度以及术后 6 小时、干预后第五天和第十天的伤口愈合过程来收集数据。此外，还使用了视觉模拟量表和红肿、水肿、瘀斑、脱落和接近度来检查疼痛强度和愈合过程。数据分析采用了 R 软件（4.0.5 版）。统计显著性水平为 P < 0.05。此外，三组的平均疼痛强度评分在统计学上也无明显差异。接受洋甘菊治疗的组在第 10 天的伤口愈合情况最好（P < 0.05）。植物化学调查显示，洋甘菊环氧乙烷中含有α-氧化二羟基苯乙醇A、α-氧化二羟基苯乙醇B、β-法呢烯和洋甘菊烯，桃金娘环氧乙烷中含有α-蒎烯、1,8-松油醇、芳樟醇、α-松油醇、乙酸芳樟醇酯和柠檬烯。

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引用次数: 0

Phytochemical analysis and in vitro anti-inflammatory, anticancer activities of Marrubium lutescens on melanoma cancer cell line and molecular docking studies 马钱子对黑色素瘤癌细胞系的植物化学分析、体外抗炎和抗癌活性以及分子对接研究

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine

Pub Date : 2024-06-27 DOI: 10.1016/j.hermed.2024.100907

Cengiz Sarikurkcu, Sevim F. Erdoğmuş, Tuğba Yazar

Introduction

The use of plants and their derivatives in the search for new therapeutic agents is on the increase every day due to their versatile applications.

Methods

Phytochemical analysis of Marrubium lutescens extract was carried out by liquid chromatography–electrospray tandem mass spectrometry (LC–ESI–MS/MS). Cytotoxicity of plant extract was determined by MTT assay on G361 cells. Moreover, the binding energetics of the two predominant compounds, rosmarinic acid and chlorogenic acid, against the target proteins microtubule affinity-regulating kinase 4 (MARK4) and human tyrosinase-related protein 1 (TYRP1) were also determined using molecular docking to gain molecular insights into the induced anti-cancer effect in G361. TAS, Total oxidant activity, and oxidative stress index of the cells lysates were determined. Also, TNF-α, TGF-β, DEF-β2, IL-1β cytokine levels were determined.

Results

Twenty distinct phytochemicals were determined and among the identified compounds, rosmarinic acid and chlorogenic acid as major components. Rosmarinic acid and chlorogenic acid have exhibited energetically highly favourable binding with intracellular target proteins MARK4 and TYRP1, as shown by their respective binding affinity values (ΔG = −7.97 kcal/mol; ΔG = −7.38 kcal/mol, respectively), suggesting further molecular evidence for the induced anti-cancer effect observed in the G361 cell line. Also, we have evaluated the anticancer prospective of M. lutescens. Anti-inflammatory mediators and oxidative stress parameters were increased to plant extract exposed cell line.

Conclusions

Reported results show that plant extract shown anticancer activity on G361 cells. According to the docking results, rosmarinic acid and chlorogenic acid, the two predominant compounds identified in the extract, may be responsible for the anti-cancer effect through synergistic action via prominent inhibition of MARK4 and TYRP1.

由于植物及其衍生物的用途广泛，人们越来越多地使用它们来寻找新的治疗药物。提取物的植物化学分析采用液相色谱-电喷雾串联质谱法（LC-ESI-MS/MS）进行。通过 MTT 法测定了植物提取物对 G361 细胞的细胞毒性。此外，还利用分子对接法测定了两种主要化合物迷迭香酸和绿原酸与靶蛋白微管亲和性调节激酶 4（MARK4）和人酪氨酸酶相关蛋白 1（TYRP1）的结合能，从而从分子角度了解了诱导 G361 细胞抗癌的作用。还测定了细胞裂解物的 TAS、总氧化活性和氧化应激指数。此外，还测定了 TNF-α、TGF-β、DEF-β2、IL-1β 细胞因子的水平。测定了 20 种不同的植物化学物质，在已确定的化合物中，香豆酸和绿原酸是主要成分。迷迭香酸和绿原酸与细胞内靶蛋白 MARK4 和 TYRP1 的结合亲和值（Δ=-7.97 kcal/mol；Δ=-7.38 kcal/mol，分别为 7.97 kcal/mol、7.38 kcal/mol）表明，它们与细胞内靶蛋白 MARK4 和 TYRP1 的结合能量非常高，这为在 G361 细胞系中观察到的诱导抗癌效应提供了进一步的分子证据。此外，我们还评估了暴露于植物提取物的细胞系的抗炎介质和氧化应激参数的抗癌前景。报告结果表明，植物提取物对 G361 细胞具有抗癌活性。根据对接结果，提取物中的两种主要化合物迷迭香酸和绿原酸可能是通过协同作用突出抑制 MARK4 和 TYRP1 而产生抗癌效果的。

{"title":"Phytochemical analysis and in vitro anti-inflammatory, anticancer activities of Marrubium lutescens on melanoma cancer cell line and molecular docking studies","authors":"Cengiz Sarikurkcu, Sevim F. Erdoğmuş, Tuğba Yazar","doi":"10.1016/j.hermed.2024.100907","DOIUrl":"10.1016/j.hermed.2024.100907","url":null,"abstract":"<div><h3>Introduction</h3><p>The use of plants and their derivatives in the search for new therapeutic agents is on the increase every day due to their versatile applications.</p></div><div><h3>Methods</h3><p>Phytochemical analysis of <em>Marrubium lutescens</em> extract was carried out by liquid chromatography–electrospray tandem mass spectrometry (LC–ESI–MS/MS). Cytotoxicity of plant extract was determined by MTT assay on G361 cells. Moreover, the binding energetics of the two predominant compounds, rosmarinic acid and chlorogenic acid, against the target proteins microtubule affinity-regulating kinase 4 (MARK4) and human tyrosinase-related protein 1 (TYRP1) were also determined using molecular docking to gain molecular insights into the induced anti-cancer effect in G361. TAS, Total oxidant activity, and oxidative stress index of the cells lysates were determined. Also, TNF-α, TGF-β, DEF-β2, IL-1β cytokine levels were determined.</p></div><div><h3>Results</h3><p>Twenty distinct phytochemicals were determined and among the identified compounds, rosmarinic acid and chlorogenic acid as major components. Rosmarinic acid and chlorogenic acid have exhibited energetically highly favourable binding with intracellular target proteins MARK4 and TYRP1, as shown by their respective binding affinity values (Δ<em>G</em>  =−7.38 kcal/mol, respectively), suggesting further molecular evidence for the induced anti-cancer effect observed in the G361 cell line. Also, we have evaluated the anticancer prospective of <em>M. lutescens</em>. Anti-inflammatory mediators and oxidative stress parameters were increased to plant extract exposed cell line.</p></div><div><h3>Conclusions</h3><p>Reported results show that plant extract shown anticancer activity on G361 cells. According to the docking results, rosmarinic acid and chlorogenic acid, the two predominant compounds identified in the extract, may be responsible for the anti-cancer effect through synergistic action <em>via</em> prominent inhibition of MARK4 and TYRP1.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"46 ","pages":"Article 100907"},"PeriodicalIF":2.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141574452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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