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2010 4th International Conference on Bioinformatics and Biomedical Engineering最新文献

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Evaluation of Groundwater Environment Carrying Capacity Based on Catastrophe Theory 基于突变理论的地下水环境承载力评价
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5515459
Nanxiang Chen, Ji-hong Qu, Jianxin Xu, Zhi-ping Li, Li Yang
Evaluation of groundwater environment carrying capacity is a multi-objective, multi-criteria and multi-level comprehensive issue. Evaluation index system of groundwater environment carrying capacity was built and divided into some subsystems in accordance with each index's inherent stratification link. Every index's effect on carrying capacity system was obtained by normalization operations catastrophe-based, so the ultimate value of fuzzy membership function catastrophe-based, which explains the groundwater environment carrying capacity, can been calculated from the lowermost layer to the topmost layer. The method was applied to evaluate groundwater environment carrying capacity in People's Victory Canal Irrigation District. In order to reveal outer and inner factor's effect to groundwater system, evaluation index system was divided into 3 kinds of top-level subsystem, i.e., the development and utilization degree of water resources, economy society and eco-environment subsystem, which include 17 kinds of bottom-level sub-index. The comprehensive evaluation result catastrophe-based is 0.9282, 0.9212, and 0.9150 in 1995, 2000, and 2005 separately, and groundwater environment carrying capacity are all weak and shows a downtrend year by year along with rapid economy expanding. The result reveals that it is a fact that groundwater resources and eco-environment in irrigation district is increasingly prominent. As it is not necessary to define the weight of evaluation indexes in dynamic fuzzy comprehensive evaluation model catastrophe-based, subjectivity of the decision-making has been reduced. The examples show that the method in this paper is reasonable and feasible by fuzzy comprehensive evaluation method's validation.
地下水环境承载力评价是一个多目标、多标准、多层次的综合问题。建立了地下水环境承载力评价指标体系,并按照各指标的内在分层联系将其划分为若干个子系统。通过基于灾变的归一化运算得到各指标对地下水环境承载力系统的影响,从而可以从最下层到最上层计算解释地下水环境承载力的模糊隶属函数灾变的极值。应用该方法对人民胜利渠灌区地下水环境承载力进行了评价。为了揭示外部因素和内部因素对地下水系统的影响,将评价指标体系划分为水资源开发利用程度、经济社会和生态环境3个顶层子系统,其中包括17个底层子指标。1995年、2000年和2005年基于灾变的综合评价结果分别为0.9282、0.9212和0.9150,随着经济的快速发展,地下水环境承载力均较弱,并呈逐年下降趋势。结果表明,灌区地下水资源和生态环境问题日益突出是一个事实。基于灾变的动态模糊综合评价模型由于不需要确定评价指标的权重,降低了决策的主观性。实例通过模糊综合评判法的验证,证明了本文方法的合理性和可行性。
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引用次数: 3
Safety Early-Warning Model for Coal Mines Based on Artificial Neural Network 基于人工神经网络的煤矿安全预警模型
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5518062
Q. Zeng, Xu Wang
With the rapid development of economy part of which is devoted by coal mines, safety evaluation can not be the perfect method to promote the safety level of coal industry especially when factors holding back safe production come up. Thus the early-warning system must be established so as to send out signals before disaster happens. Artificial neural network is the basis in this article to set up early-warning system for coal mines to save both human lives and industry property.
随着煤矿经济的快速发展,特别是在制约煤矿安全生产的因素出现的情况下,安全评价已不是提高煤矿安全水平的完美方法。因此,必须建立预警系统,在灾害发生前发出信号。本文以人工神经网络为基础,建立煤矿事故预警系统,以挽救生命和工业财产。
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引用次数: 0
Effects of Sub-Chronic Aluminum Intoxication on Apoptosis of Cerebrum Neurocytes in Chickens: Preliminary Study on Effect of Aluminum Accumulation on Nervous System in Chickens 亚慢性铝中毒对鸡大脑神经细胞凋亡的影响:铝蓄积对鸡神经系统影响的初步研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5515279
Hao Sun, Yan-fei Li, Chong-wei Hu, Y. She, Lingyu Gao
The model of different level's sub-chronic aluminum intoxication in chickens was established by continuous peritoneal injection of fixed volume and different gradient aluminum trichloride (AlCl3). The apoptosis of cerebrum neurocytes was detected by agarose gel electrophor-esis, AO/EB double fluorescent staining and flow cytometry. The results showed that: (1) The typical apoptosis and necrosis of cerebrum neurocytes were observed in the fluorescence microscope, and the apoptotic index in the experimental groups was significantly higher than that in the control groups (P<0.01), which increased along with increase of aluminum concentration; (2) The number of cerebrum neurocytes increased in G0/G1 phases, while decreased in G2/M and S phases. The cell proliferation index decreased and apoptosis rate increased, which were significantly different compared with the control ones (P<0.01); (3) The typical DNA Ladder-shaped of cerebrum neurocytes of chickens in experimental groups was displayed by agarose gel electropho- resis. The results indicated that aluminum can cause the DNA damage, inhibite the proliferation and induce the apoptosis of cerebrum cell.
采用固定体积、不同梯度三氯化铝(AlCl3)连续腹腔注射的方法,建立了鸡不同程度亚慢性铝中毒模型。采用琼脂糖凝胶电泳、AO/EB双荧光染色和流式细胞术检测大脑神经细胞凋亡情况。结果表明:(1)荧光显微镜下观察到典型的脑神经细胞凋亡和坏死,且各实验组的凋亡指数显著高于对照组(P<0.01),且随铝浓度的升高而升高;(2)大脑神经细胞数量在G0/G1期增加,在G2/M期和S期减少。细胞增殖指数降低,细胞凋亡率升高,与对照组相比差异极显著(P<0.01);(3)琼脂糖凝胶电泳显示了实验组鸡大脑神经细胞典型的DNA阶梯状结构。结果表明,铝能引起大脑细胞DNA损伤,抑制细胞增殖,诱导细胞凋亡。
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引用次数: 1
Synthesis and Performance Research of Biodegradable Modified Polyaspartic Acid 可生物降解改性聚天冬氨酸的合成及性能研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516477
Yu-hua Gao, Zhenfa Liu, Lihui Zhang, Yanji Wang
Modified polyaspartic acid (PASP-SEA-ASP) was prepared from polysuccinimide (PSI) that was thermal condensation polymer of aspartic acid (ASP), taurine (SEA) and ASP. The property of scale inhibition, dispersant and biodegradation of PASP-SEA-ASP were studied. The structure of modified polyaspartic acid was characterized by means of FTIR. Calcium carbonate crystals in scale samples were analysed by means of SEM. The results showed that under Ca2+ 400 mg/L, HCO3- 800 mg/L and PASP-SEA-ASP composite 4 mg/L, the scale inhibition rate of PASP-SEA-ASP was 67%. Under PASP-SEA-ASP composite 15 mg/L, the least diaphaneity of the top liquid on dispersing iron oxide was 53.8 %. And PASP-SEA-ASP possess better biodegradation performance and the highest biodegradation rate can reach 70%. Modified polyaspartic acid could distort calcium carbonate crystallite and made calcium carbonate crystallite more dispersive.
以天冬氨酸(ASP)、牛磺酸(SEA)和ASP的热缩合聚合物聚琥珀酰亚胺(PSI)为原料,制备了改性聚天冬氨酸(PASP-SEA-ASP)。研究了PASP-SEA-ASP的阻垢、分散剂和生物降解性能。用红外光谱对改性聚天冬氨酸的结构进行了表征。用扫描电子显微镜对水垢样中的碳酸钙晶体进行了分析。结果表明,在Ca2+ 400 mg/L、HCO3- 800 mg/L和PASP-SEA-ASP复合4 mg/L条件下,PASP-SEA-ASP的阻垢率为67%。在PASP-SEA-ASP复合物浓度为15 mg/L时,分散氧化铁的上液透明度最低为53.8%。PASP-SEA-ASP具有较好的生物降解性能,最高生物降解率可达70%。改性聚天冬氨酸能使碳酸钙晶体变形,使碳酸钙晶体更加分散。
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引用次数: 9
Acute Effects of Oil Dispersant and No.0 Diesel Oil on Sea Urchin, Hemicentrotus pulcherrimus 油类分散剂和0号柴油对海胆的急性影响
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5515278
F. Lv, D. Xiong
The acute toxicities of water-accomodated fractions(WAFs) of No.0 diesel oil and dispersed water-accomodated fractions(dis-WAFs) on sea urchin, Hemicentrotus pulcherrimus were studied and tissue-specific response against oxidative stress were analyzed in this paper. Dispersed WAFs of No.0 diesel oil was found to be more toxic to sea urchin than WAFs. 24, 48, 72, 96-h EC50 values of WAFs were 18.2 mg/L, 15.5 mg/L, 11.5 mg/L and 9.5mg/L respectively; and those of dis-WAFs were 11.7 mg/L, 9.1mg/L, 7.4mg/L and 5.1mg/L respectively. GC-MS analysis showed that more aromatic compounds dissolve into the sea water after adding chemical dispersant to oil. Followed by the exposure time, superoxide dismutase (SOD), catalase(CAT) and glutathione S-transferase(GST) activities in intestine were induced first, and then decreased gradually. The dose-effect relationship occurrs in 13d samples; SOD , CAT and GST activities of all concentration groups were recovered to the corresponding control level after 11d recovery experiment. The activities first increased in short time at low concentration of WAFs, and was significantly depressed at high concentrations. SOD, CAT and GST had important roles in detoxification, and showed great potential as biomarkers for benthic marine oil pollution.
本文研究了0号柴油水溶馏分(WAFs)和分散水溶馏分(dis-WAFs)对海胆的急性毒性作用,并分析了它们对氧化应激的组织特异性反应。发现0号柴油中分散的waf对海胆的毒性大于waf。24、48、72、96 h的EC50值分别为18.2 mg/L、15.5 mg/L、11.5 mg/L和9.5mg/L;disf - was分别为11.7 mg/L、9.1mg/L、7.4mg/L和5.1mg/L。气相色谱-质谱分析表明,在石油中加入化学分散剂后,更多芳香族化合物溶解到海水中。随着暴露时间的延长,肠道超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽s -转移酶(GST)活性先被诱导,后逐渐降低。在13d样品中出现剂量效应关系;11d恢复试验后,各浓度组SOD、CAT和GST活性均恢复到相应的对照水平。在低浓度条件下,活性先在短时间内升高,在高浓度条件下,活性显著降低。SOD、CAT和GST在解毒过程中具有重要作用,具有作为底栖海洋油类污染生物标志物的潜力。
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引用次数: 2
The Dynamic Study in Astrocytes and Cytokines in a Rat Model of Vascular Dementia by Xiongma Dropping Pill 雄马滴丸对血管性痴呆模型大鼠星形细胞和细胞因子的动态研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5518136
Ying Zhao, Lin Liu, Dan Li, H. Teng
OBJECTIVE: To observe the change of Astrocyte (Ast) and cytokines, during vascular dementia (VD) formation to explore the prevention and treatment VD mechanisms of Xiongma dropping pill (XMDP). METHODS: To permanent bilateral ligation of the common carotid artery (2-VO) to establish a VD model. The glial fibrillary acidic protein(GFAP)on the rats brain, and IL-1β, IL-6 and TNF-α were observed by immunohistochemical staining and radioimmunoassay after administration 30 and 60 days. RESULTS: Compared with the sham-operation group, the number of GFAP positive cells and the contents of IL-1β, IL-6 and TNF-α were increased in the model rats (P < 0.05 or P < 0.01). Compared with the model group, XMDP and Ginkgo leaf tablets group could active or proliferative hippocampus and frontal cortex Asts, decrease the number of GFAP positive cells(P < 0.05), and decrease the contents of IL-1β, IL-6 and TNF-α(P < 0.05 or P < 0.01). In addition, the contents of IL-1β, IL-6 and TNF-α in all XMDP groups, after 60 days of administration, were remarkably greater than corresponding concentrations at 30 days(P < 0.05 or P < 0.01). CONCLUSION: XMDP could prevent and treatment VD may be associated with inhibiting the excessive activation or proliferation of Ast and to reduce the generation of the cytokines.
目的:观察血管性痴呆(VD)形成过程中星形胶质细胞(Ast)和细胞因子的变化,探讨雄马滴丸(XMDP)防治VD的作用机制。方法:双侧永久性结扎颈总动脉(2-VO)建立VD模型。大鼠脑上的胶质纤维酸性蛋白(GFAP) IL-1β IL-6和TNF-α给药30、60 d后进行免疫组化染色和放射免疫分析。结果:与假手术组比较,GFAP阳性细胞数及IL-1β、IL-6、TNF-α含量;(P < 0.05或P < 0.01)。与模型组比较,XMDP和银杏叶片组均能激活或增殖海马和额叶皮层ast,减少GFAP阳性细胞数量(P < 0.05),降低il -1、IL-6、TNF-α含量(P < 0.05或P < 0.01)。此外,IL-1β、IL-6和TNF-α给药60 d后,各XMDP组均显著或极显著高于给药30 d时的相应浓度(P < 0.05或P < 0.01)。结论:XMDP具有预防和治疗VD的作用,可能与抑制Ast的过度活化或增殖、减少细胞因子的产生有关。
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引用次数: 0
Comparison Study of Peptide Retention Time Prediction Model Based on Five Kinds of Amino Acid Descriptors in HPLC by Support Vector Machine 基于五种氨基酸描述符的高效液相色谱多肽保留时间预测模型的支持向量机比较研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516374
Jiajian Yin
Based on amino acid descriptors(z-scales, c-scales, ISA-ECI,MS-WHIM and PRIN) and additive method, evaluation of predict performance of five amino acid descriptors in peptide QSRR(Quantitative structure-retention relationships) with 101 promiscuous peptides in High-Performance Liquid Chromato- graphy by support vector regression(SVR) is made in the article, and RBF(radical basis function) is selected as kernel function. Using leave-one-out cross-validation (LOO-CV), we suppose that predicting accuracy of ISA-ECI is better than the other descriptors in SVR with RBF. The prediction correlation coefficient of the SVR model (ε = 0.001,σ= 5 and C= 100) is 0.8445 by leave-one-out cross validation. The standard error of prediction (SEP) error of the dataset is 1.03 by fitting calculation, and the prediction correlation coefficient is 0.9642.The prediction results are in agreement with the experimental values. This paper provided a simple and effective method for predicting the retention behavior of peptide and some insight into what structural features are related to the retention time of peptides. Moreover, it also offered an idea about nonlinear relation between retention time of peptides and their structural descriptors (ISA-ECI).Therefore, SVR is assumed to be a feasible method in peptide QSAR (Quantitative structure-activity relationships) model.
基于氨基酸描述符(z-scale、c-scale、ISA-ECI、MS-WHIM和PRIN)和加性法,采用支持向量回归(SVR)评价了5个氨基酸描述符在高效液相色谱中对101个混杂肽的QSRR(定量结构-保留关系)预测性能,并选择RBF(radical basis function)作为核函数。利用留一交叉验证(LOO-CV),我们假设在RBF的SVR中,ISA-ECI的预测精度优于其他描述符。SVR模型的预测相关系数(ε= 0.001,σ= 5, C= 100)通过留一交叉验证为0.8445。经拟合计算,该数据集的预测标准差(SEP)误差为1.03,预测相关系数为0.9642。预测结果与实验值吻合较好。本文提供了一种简单有效的预测多肽保留行为的方法,并对多肽的结构特征与保留时间的关系有了一些认识。此外,还提出了多肽保留时间与其结构描述符(ISA-ECI)之间的非线性关系。因此,假设SVR是一种可行的多肽QSAR(定量构效关系)模型方法。
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引用次数: 0
Electroencephalograph Bicoherence for Assessing the Analgesia of Acupuncture 脑电图双相干性评价针刺镇痛作用
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516448
Ji-jun Tong, Yaming Wang, Yingying Lv, Huade Chen
n the past decades of years the systematic research on pain mechanisms has been the focus of scientists. The traditional Chinese medicine such as acupuncture has shown great potential in pain relief. Transcutaneous Electrical Acupoint Stimulation (TEAS) has been used and bicoherence of EEG has been extracted and used as evaluation criterion of pain relief in the research. In this study 12 volunteers were enrolled and each one received pain stimulation and attended two group experiments, one was transcutaneous electrical stimulation on Hegu acupoint (TEAS), the other was control group (no stimulation). The EEG was recorded and the volume of bicoherence (BIC_V) was extracted, while attended VAS test. The BIC_V increased after pain stimulation, especially in certain cerebral location (Fp1,P3,O1,O2,T6). The BIC_V decreased larger in extent in TEAS group than control group, which has the same change trend with VAS. The study primary proved the efficiency of acupuncture analgesia and BIC_V could be used as an objective assessment criterion of pain perception.
在过去的几十年里,对疼痛机制的系统研究一直是科学家关注的焦点。针灸等传统中医在缓解疼痛方面显示出巨大的潜力。本研究采用经皮穴位电刺激(TEAS),提取脑电图的双相干性,并将其作为疼痛缓解的评价标准。本研究共招募12名志愿者,每人接受疼痛刺激,分为两组实验,一组为经皮电刺激合谷穴(TEAS),另一组为对照组(无刺激)。记录脑电图,提取双相干体积(BIC_V),同时进行VAS测试。疼痛刺激后BIC_V增加,特别是在大脑的某些部位(Fp1,P3,O1,O2,T6)。tea组BIC_V下降幅度大于对照组,与VAS变化趋势相同。本研究初步证明针刺镇痛效果及BIC_V可作为疼痛感知的客观评价标准。
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引用次数: 0
Dissolution Characteristics of Limestone with Organic Acid 石灰石与有机酸的溶解特性
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516025
Shengyu Liu, W. Qiu, Bin Qu, Y. Xu
The effects of organic acids on limestone dissolution rate were investigated by pH-stat method. The results showed that the concentration of organic acid, the acidity of limestone slurry and the kind of organic acid were the main factor to increase limestone dissolution rate. By increasing concentration of organic acid added, the granular limestone can be used as a desulfurizing reagent. Thus, the energy of the pulverizing limestone can be reduced. Furthermore, the mechanism of organic acid promoting limestone dissolution was suggested.
采用pH-stat法研究了有机酸对石灰石溶解速率的影响。结果表明,有机酸浓度、灰岩浆的酸度和有机酸种类是提高灰岩溶解速率的主要因素。通过增加有机酸的添加浓度,颗粒状石灰石可作为脱硫剂。因此,可以降低石灰石粉化的能量。进一步探讨了有机酸促进石灰石溶解的机理。
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引用次数: 0
Study on the Effects of Ecological Engineering for Ecological Restoration on Bacterial Community Structure by Molecular Biological Method in a Eutrophic Lake 富营养化湖泊生态修复工程对细菌群落结构影响的分子生物学研究
Pub Date : 2010-06-18 DOI: 10.1109/ICBBE.2010.5516097
Sheng Feng, Dinglong Li, J. Ma
Abstract:Study on microbial community structure and diversity in freshwater is important for exploiting the relationship of microbial community and its habitats, predicting and monitoring the ecological changing of contaminated water habitat. The retrieval of 16SrDNA genes directly from the environment has made it possible to estimate the phylogenetic diversity of a natural bacterial community without preceding cultivation steps, which reflect more comprehensively of bacterial community in the water samples in different ecological restoration areas. The genomic DNA was extracted from water, PCR amplification of this gene fragments; DGGE was analyzed; the abundance of bacteria were observed. The result showed that the bacterial community and abundance changed in different ecological restoration sections. With the depth of bio-disposal, the diversity of microbe community increased, however, the abundance of bacteria declined, which demonstrated the ecological restoration engineering improved the water habitat, promoted the microbe community diversity in the eco-restoration areas.
摘要淡水中微生物群落结构和多样性的研究对于揭示微生物群落与其生境的关系,预测和监测污染水体生境的生态变化具有重要意义。直接从环境中提取16SrDNA基因,使得无需预先培养就能估算天然细菌群落的系统发育多样性成为可能,更全面地反映了不同生态修复区水样中细菌群落的情况。从水中提取基因组DNA, PCR扩增该基因片段;分析DGGE;观察了细菌的丰度。结果表明,不同生态恢复区段的细菌群落和丰度发生了变化。随着生物处理深度的增加,微生物群落多样性增加,但细菌丰度下降,说明生态修复工程改善了水体生境,促进了生态修复区内微生物群落多样性。
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引用次数: 0
期刊
2010 4th International Conference on Bioinformatics and Biomedical Engineering
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