Tung Nguyen-Thanh, Bao-Song Nguyen-Tran, S. Cruciani, Thuan Dang-Cong, M. Maioli
Articular cartilage degeneration represents one of the main features of osteoarthritis. Recently, novel approaches based on biomaterials have been successfully applied to osteochondral regeneration. Our study was carried out on rabbits to assess a model of articular cartilage damage to test biomaterials for osteochondral regeneration. We created osteochondral defects on the surface of the trochlear groove area of the femurs in 15 white male New Zealand rabbits of the size of 3 mm × 3 mm (diameter × depth). Rabbits were then monitored and samples were collected 2 weeks, 4 weeks, and 6 weeks after the operation. The reconstruction of defects was assessed macroscopically according to the International Cartilage Repair Society (ICRS) scale and radiography (X-ray). For microscopic evaluation, haematoxylin-eosin staining and safranin O staining were used. The defects were repaired by regenerative tissue, and the recovery results gradually increased after 2 weeks, 4 weeks, and 6 weeks, showing both microscopically and macroscopically. However, the regenerative tissue was mainly fibrous connective tissue, not cartilage or bone. This is a model of articular cartilage damage that is suitable for early screening of preclinical studies related to osteochondral regeneration using biomaterials.
{"title":"A rabbit femoral trochlear defect model for chondral and osteochondral regeneration","authors":"Tung Nguyen-Thanh, Bao-Song Nguyen-Tran, S. Cruciani, Thuan Dang-Cong, M. Maioli","doi":"10.2754/avb202291030293","DOIUrl":"https://doi.org/10.2754/avb202291030293","url":null,"abstract":"Articular cartilage degeneration represents one of the main features of osteoarthritis. Recently, novel approaches based on biomaterials have been successfully applied to osteochondral regeneration. Our study was carried out on rabbits to assess a model of articular cartilage damage to test biomaterials for osteochondral regeneration. We created osteochondral defects on the surface of the trochlear groove area of the femurs in 15 white male New Zealand rabbits of the size of 3 mm × 3 mm (diameter × depth). Rabbits were then monitored and samples were collected 2 weeks, 4 weeks, and 6 weeks after the operation. The reconstruction of defects was assessed macroscopically according to the International Cartilage Repair Society (ICRS) scale and radiography (X-ray). For microscopic evaluation, haematoxylin-eosin staining and safranin O staining were used. The defects were repaired by regenerative tissue, and the recovery results gradually increased after 2 weeks, 4 weeks, and 6 weeks, showing both microscopically and macroscopically. However, the regenerative tissue was mainly fibrous connective tissue, not cartilage or bone. This is a model of articular cartilage damage that is suitable for early screening of preclinical studies related to osteochondral regeneration using biomaterials.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Risvanli, T. Safak, O. Yılmaz, Burak Yuksel, M. Kilinc, I. Seker
This study was aimed to reveal the relationship between the B-mode and colour Doppler ultrasonography features of the portio vaginalis of the cervix in Simmental cattle with different physiological and pathological conditions of genital organs. For this purpose, 90 cattle were used in the study. The length, width and circumference of the portio vaginalis of the cervix were measured ultrasonographically using a 5 MHz linear probe. In addition, the portio vaginalis of the cervix was ultrasonographically evaluated in terms of echogenicity, cystic formations, calcification and integrity of its borders. Regarding the colour Doppler ultrasonography, measurements of the vascular distribution category, vascular density and the vascular morphological appearance were made. In this study, it was found that the rate of irregularity of the border of the portio vaginalis in B-mode ultrasonography according to the pregnancy status was 10.9% in non-pregnant cows, while this was not observed in pregnant cows (0.0%). According to the metritis status, it was observed that the rate of regularity of the border of the portio vaginalis was higher in animals with metritis (45.5%) in B-mode ultrasonography. As a result, it was concluded that there were significant changes in the B-mode and colour Doppler ultrasonography characteristics of the portio vaginalis of the cervix in different physiological and pathological conditions in cattle, and that hypotheses can be developed regarding the fertility of the animals by evaluating these characteristics.
{"title":"Evaluation of the portio vaginalis of the cervix by B-mode and colour Doppler ultrasound in Simmental cattle","authors":"A. Risvanli, T. Safak, O. Yılmaz, Burak Yuksel, M. Kilinc, I. Seker","doi":"10.2754/avb202291010003","DOIUrl":"https://doi.org/10.2754/avb202291010003","url":null,"abstract":"This study was aimed to reveal the relationship between the B-mode and colour Doppler ultrasonography features of the portio vaginalis of the cervix in Simmental cattle with different physiological and pathological conditions of genital organs. For this purpose, 90 cattle were used in the study. The length, width and circumference of the portio vaginalis of the cervix were measured ultrasonographically using a 5 MHz linear probe. In addition, the portio vaginalis of the cervix was ultrasonographically evaluated in terms of echogenicity, cystic formations, calcification and integrity of its borders. Regarding the colour Doppler ultrasonography, measurements of the vascular distribution category, vascular density and the vascular morphological appearance were made. In this study, it was found that the rate of irregularity of the border of the portio vaginalis in B-mode ultrasonography according to the pregnancy status was 10.9% in non-pregnant cows, while this was not observed in pregnant cows (0.0%). According to the metritis status, it was observed that the rate of regularity of the border of the portio vaginalis was higher in animals with metritis (45.5%) in B-mode ultrasonography. As a result, it was concluded that there were significant changes in the B-mode and colour Doppler ultrasonography characteristics of the portio vaginalis of the cervix in different physiological and pathological conditions in cattle, and that hypotheses can be developed regarding the fertility of the animals by evaluating these characteristics.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Karakurt, E. Beytut, S. Dağ, H. Nuhoğlu, A. Yıldız, Emre Kurtbaş
This study aimed to reveal the presence of reactive oxygen species (ROS)-induced lipid peroxidation and DNA damage in ovine pulmonary adenocarcinomas (OPA) by evaluating malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expressions by immunohistochemical and immunofluorescence methods. Lung tissue samples were collected from 26 sheep brought to the Pathology Department for routine diagnosis. Lung tissues were fixed in 10% neutral buffered formalin, following routine procedures tissues were stained with hematoxylin and eosin. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Indirect immunofluorescence method was applied to the sections. Tumoral cells showed acinar, papillary or mixed type patterns. Only 2 of 20 cases metastasized to regional lymph nodes. All OPAs were immune positive for Jaagsiekte Sheep Retrovirus Capsid Protein (JSRV CA), MDA and 8-OHdG. The control group was negative for JSRV CA, MDA and 8-OHdG expressions. Malondialdehyde and 8-OHdG immune positive cells were statistically increased in the OPA group compared to the control group. In conclusion, our results demonstrate that higher MDA and 8-OHdG expressions in sheep with OPA suggest that OPA may be closely related to lipid peroxidation and oxidative DNA damage.
{"title":"Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods","authors":"E. Karakurt, E. Beytut, S. Dağ, H. Nuhoğlu, A. Yıldız, Emre Kurtbaş","doi":"10.2754/avb202291030235","DOIUrl":"https://doi.org/10.2754/avb202291030235","url":null,"abstract":"This study aimed to reveal the presence of reactive oxygen species (ROS)-induced lipid peroxidation and DNA damage in ovine pulmonary adenocarcinomas (OPA) by evaluating malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expressions by immunohistochemical and immunofluorescence methods. Lung tissue samples were collected from 26 sheep brought to the Pathology Department for routine diagnosis. Lung tissues were fixed in 10% neutral buffered formalin, following routine procedures tissues were stained with hematoxylin and eosin. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Indirect immunofluorescence method was applied to the sections. Tumoral cells showed acinar, papillary or mixed type patterns. Only 2 of 20 cases metastasized to regional lymph nodes. All OPAs were immune positive for Jaagsiekte Sheep Retrovirus Capsid Protein (JSRV CA), MDA and 8-OHdG. The control group was negative for JSRV CA, MDA and 8-OHdG expressions. Malondialdehyde and 8-OHdG immune positive cells were statistically increased in the OPA group compared to the control group. In conclusion, our results demonstrate that higher MDA and 8-OHdG expressions in sheep with OPA suggest that OPA may be closely related to lipid peroxidation and oxidative DNA damage.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lehmann, L. Anskienė, J. Sabeckienė, N. Sutkevičienė
Collection of epididymal stallion sperm offers the opportunity to retain and use genetic material from the males after elective castration or even post mortem. The aim of the present study was to evaluate the effect of the Ringer B. Braun solution on stallion epididymal sperm viability and motility during liquid storage for 72 h at 4 °C and at 20 °C. Eight stallions (3–10 years old) were used in the study. Sperm from the cauda epididymis was harvested immediately after routine castration. The sperm from epididymis was washed out and diluted with Ringer B. Braun solution and with a commercial semen extender which was used as a control. Samples from each horse were divided into two parts: one part of samples was stored at 4 ± 1 °C as recommended for the commercial semen extender, the other one was stored at 20 ± 1 °C. Sperm viability, motility, and pH were checked one hour after collection and dilution, and after 24, 48 and 72 h of incubation. The results of sperm viability, subjective and progressive sperm motility showed slightly higher results in semen samples diluted with Ringer B. Braun solution in one hour compared to the commercial extender, by 2.40 ± 0.49% for viability (P > 0.05), by 0.30 ± 9.92% for subjective motility (P > 0.05) and by 5.70 ± 6.07% for progressive motility (P > 0.05). We suggest that Ringer solution could be used for a short term storage (1–24 h) of equine epididymal semen at a 4 °C temperature.
{"title":"Efficiency of Ringer B. Braun solution on stallion epididymal sperm motility and viability compared to the commercial extender within 72 hours of storage","authors":"A. Lehmann, L. Anskienė, J. Sabeckienė, N. Sutkevičienė","doi":"10.2754/avb202291040355","DOIUrl":"https://doi.org/10.2754/avb202291040355","url":null,"abstract":"Collection of epididymal stallion sperm offers the opportunity to retain and use genetic material from the males after elective castration or even post mortem. The aim of the present study was to evaluate the effect of the Ringer B. Braun solution on stallion epididymal sperm viability and motility during liquid storage for 72 h at 4 °C and at 20 °C. Eight stallions (3–10 years old) were used in the study. Sperm from the cauda epididymis was harvested immediately after routine castration. The sperm from epididymis was washed out and diluted with Ringer B. Braun solution and with a commercial semen extender which was used as a control. Samples from each horse were divided into two parts: one part of samples was stored at 4 ± 1 °C as recommended for the commercial semen extender, the other one was stored at 20 ± 1 °C. Sperm viability, motility, and pH were checked one hour after collection and dilution, and after 24, 48 and 72 h of incubation. The results of sperm viability, subjective and progressive sperm motility showed slightly higher results in semen samples diluted with Ringer B. Braun solution in one hour compared to the commercial extender, by 2.40 ± 0.49% for viability (P > 0.05), by 0.30 ± 9.92% for subjective motility (P > 0.05) and by 5.70 ± 6.07% for progressive motility (P > 0.05). We suggest that Ringer solution could be used for a short term storage (1–24 h) of equine epididymal semen at a 4 °C temperature.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Experiments assessed the comparative efficacy of oxalic acid solution (OA) and combination of formic acid (FA) evaporation and trickling OA to control the honey bee mite, Varroa destructor, at two apiary locations. Queen caging, consecutive OA treatments of broodless colonies, or combined treatments using OA and FA in colonies with brood increased mite mortality (P < 0.05) in comparison to the pre-treatment period. FA application in colonies with brood in September and October resulted in an average mite mortality of 18% in the first apiary and subsequent FA applications in the same period in the second apiary killed, on average, 73% and 71% of mites; respectively. OA treatment of broodless colonies after queen caging at two apiaries resulted in 18% and 47% mite mortality. Caging the queens and OA treatments in broodless colonies or subsequently use consecutive OA or FA treatments ensure adequate mite reduction before wintering the colonies. Synergistic control methods of Varroa mites using OA and FA along with queen caging is discussed.
{"title":"Sustainable varroa mite (Varroa destructor) control in field conditions","authors":"A. Gregorc, I. Planinc","doi":"10.2754/avb202291040401","DOIUrl":"https://doi.org/10.2754/avb202291040401","url":null,"abstract":"Experiments assessed the comparative efficacy of oxalic acid solution (OA) and combination of formic acid (FA) evaporation and trickling OA to control the honey bee mite, Varroa destructor, at two apiary locations. Queen caging, consecutive OA treatments of broodless colonies, or combined treatments using OA and FA in colonies with brood increased mite mortality (P < 0.05) in comparison to the pre-treatment period. FA application in colonies with brood in September and October resulted in an average mite mortality of 18% in the first apiary and subsequent FA applications in the same period in the second apiary killed, on average, 73% and 71% of mites; respectively. OA treatment of broodless colonies after queen caging at two apiaries resulted in 18% and 47% mite mortality. Caging the queens and OA treatments in broodless colonies or subsequently use consecutive OA or FA treatments ensure adequate mite reduction before wintering the colonies. Synergistic control methods of Varroa mites using OA and FA along with queen caging is discussed.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Codl, J. Ducháček, M. Vacek, J. Pytlík, L. Stádník, M. Vrhel
The aim of this study was to evaluate the amounts of time that selected crossbreeds of Holstein (H), Red-Holstein (R), and Czech Fleckvieh (C) cattle spent chewing, eating, being physically active using a Vitalimetr 5P neck responder during the period around oestrus in all seasons of the year. Oestrus and the days preceding it greatly affected the times of events of increased activity, eating, and rumination. Eating and chewing times were longest for H cows, at 51–74%, and shortest for C cows, at > 88%. The number of events of increased activity was highest (533.4 ± 8.94 events, P < 0.01) for cows R 51–74%. Oestrus in dairy cows was manifested by decreases in the times of chewing and eating and the frequency of events of increased activity. Feeding time decreased by 8.01 min from the second day before oestrus to the day of oestrus. Rumination time also decreased from the second day before oestrus to the day of oestrus. Rumination time on the day of oestrus decreased by an average of 61.80 min but increased the next day by 33.39 min. In contrast, events of increased activity were highest on the day of oestrus (623 ± 7.68 events). Our findings on differences between breeds, lactation order, and months may contribute to the improvement of algorithms for detecting oestrus, thereby improving the economic status of farms.
{"title":"Relationship between daily activities duration and oestrus in dairy cows over the year","authors":"R. Codl, J. Ducháček, M. Vacek, J. Pytlík, L. Stádník, M. Vrhel","doi":"10.2754/avb202291010011","DOIUrl":"https://doi.org/10.2754/avb202291010011","url":null,"abstract":"The aim of this study was to evaluate the amounts of time that selected crossbreeds of Holstein (H), Red-Holstein (R), and Czech Fleckvieh (C) cattle spent chewing, eating, being physically active using a Vitalimetr 5P neck responder during the period around oestrus in all seasons of the year. Oestrus and the days preceding it greatly affected the times of events of increased activity, eating, and rumination. Eating and chewing times were longest for H cows, at 51–74%, and shortest for C cows, at > 88%. The number of events of increased activity was highest (533.4 ± 8.94 events, P < 0.01) for cows R 51–74%. Oestrus in dairy cows was manifested by decreases in the times of chewing and eating and the frequency of events of increased activity. Feeding time decreased by 8.01 min from the second day before oestrus to the day of oestrus. Rumination time also decreased from the second day before oestrus to the day of oestrus. Rumination time on the day of oestrus decreased by an average of 61.80 min but increased the next day by 33.39 min. In contrast, events of increased activity were highest on the day of oestrus (623 ± 7.68 events). Our findings on differences between breeds, lactation order, and months may contribute to the improvement of algorithms for detecting oestrus, thereby improving the economic status of farms.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Akyüz, O. Merhan, U. Aydın, M. Sezer, M. Kuru, E. Karakurt, Uğur Yıldız, K. Bozukluhan, Y. U. Batı, A. Yıldız, G. Gökce
This study aims to determine serum neopterin, procalcitonin, total sialic acid (TSA), paraoxonase-1 (PON1), and some haematological indices in calves with aspiration pneumonia (ASP). The patient group consisted of 30 calves of the Simmental breed, 0–28 days old, diagnosed with ASP upon clinical examination, anamnesis, auscultation examination, and radiographic results. The control group consisted of 10 healthy calves, otherwise with the same characteristics. A significant difference was found between calves with ASP and the control group of calves in serum iron (Fe, P < 0.001), total iron-binding capacity (TIBC, P < 0.05), PON1, TSA, procalcitonin, neopterin, and total leukocyte count (WBC) indices as a result of the analyses (P < 0.001). Serum procalcitonin concentration was found to be 285.71 ng/ml in the ASP group and 30.34 ng/ml in the control group. Serum neopterin concentration was found to be 37.68 nmol/l in the ASP group and 15.14 nmol/l in the control group. Serum procalcitonin, neopterin, and TSA concentrations were found to be significantly higher in the ASP group compared to the control group. It was concluded that biomarkers such as serum procalcitonin, neopterin, and TSA are high in calves with ASP, and as a result, these markers provide diagnostically and prognostically important information.
本研究旨在测定吸入性肺炎(ASP)犊牛血清新蝶呤、降钙素原、总唾液酸(TSA)、对氧磷酶-1 (PON1)及一些血液学指标。患者组为30头西门塔尔犊牛，0-28日龄，经临床检查、记忆、听诊及影像学检查诊断为ASP。对照组为10头健康犊牛，其余特征相同。结果表明，ASP组犊牛血清铁(Fe, P < 0.001)、总铁结合力(TIBC, P < 0.05)、PON1、TSA、降钙素原、新蝶呤、白细胞总数(WBC)等指标与对照组犊牛差异显著(P < 0.001)。ASP组血清降钙素原浓度为285.71 ng/ml，对照组为30.34 ng/ml。ASP组血清新蝶呤浓度为37.68 nmol/l，对照组为15.14 nmol/l。与对照组相比，ASP组血清降钙素原、新蝶呤和TSA浓度显著升高。结果表明，血清降钙素原、新蝶呤素和TSA等生物标志物在ASP犊牛中含量较高，这些标志物为ASP犊牛的诊断和预后提供了重要信息。
{"title":"Neopterin, procalcitonin, total sialic acid, paraoxonase-1 and selected haematological indices in calves with aspiration pneumonia","authors":"E. Akyüz, O. Merhan, U. Aydın, M. Sezer, M. Kuru, E. Karakurt, Uğur Yıldız, K. Bozukluhan, Y. U. Batı, A. Yıldız, G. Gökce","doi":"10.2754/avb202291020115","DOIUrl":"https://doi.org/10.2754/avb202291020115","url":null,"abstract":"This study aims to determine serum neopterin, procalcitonin, total sialic acid (TSA), paraoxonase-1 (PON1), and some haematological indices in calves with aspiration pneumonia (ASP). The patient group consisted of 30 calves of the Simmental breed, 0–28 days old, diagnosed with ASP upon clinical examination, anamnesis, auscultation examination, and radiographic results. The control group consisted of 10 healthy calves, otherwise with the same characteristics. A significant difference was found between calves with ASP and the control group of calves in serum iron (Fe, P < 0.001), total iron-binding capacity (TIBC, P < 0.05), PON1, TSA, procalcitonin, neopterin, and total leukocyte count (WBC) indices as a result of the analyses (P < 0.001). Serum procalcitonin concentration was found to be 285.71 ng/ml in the ASP group and 30.34 ng/ml in the control group. Serum neopterin concentration was found to be 37.68 nmol/l in the ASP group and 15.14 nmol/l in the control group. Serum procalcitonin, neopterin, and TSA concentrations were found to be significantly higher in the ASP group compared to the control group. It was concluded that biomarkers such as serum procalcitonin, neopterin, and TSA are high in calves with ASP, and as a result, these markers provide diagnostically and prognostically important information.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"45 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to determine and compare the plasma and erythrocyte (RBCs) levels of vitamin E in cows of 3 different categories (2–3 weeks antepartum, 1–3 weeks and 2.5–3 months of lactation) and 1-month-old calves on dairy nutrition. We were interested in the degree of correlation of vitamin E in plasma and RBCs within the individual categories. Blood on EDTA was collected from 61 cows and 12 calves. As a part of the haematological examination, we determined haematocrit (HCT) immediately after the collection. We determined vitamin E from plasma and RBCs by a standard HPLC method. We compared the results of vitamin E in plasma and RBCs and correlated them. The concentration of vitamin E in the plasma and RBCs was 6.98 and 3.45 μmol/l, respectively, in cows 2–3 weeks antepartum; 1–3 weeks of lactation it was 4.98 and 3.34; 2.5–3 months of lactation 11.76 and 2.80 μmol/l; and in the case of calves 12.07 and 6.29 μmol/l. Weak correlations were observed between vitamin E in plasma and the RBC concentrations in the antepartum category R2 = 0.2076; 1–3 weeks of lactation R2 = 0.0369; 2.5–3 months of lactation R2 = 0.2403 and calves on dairy nutrition R2 = 0.4628. Vitamin E concentrations in RBCs were shown to be more stable than in plasma, where the concentrations varied. It is possible that vitamin E in RBCs could tell us more about the longer-term reserves of vitamin E in the organism. The highest concentration of vitamin E in plasma and RBCs, as well as a stronger correlation was found in calves.
{"title":"Concentration of vitamin E in bovine plasma and erythrocytes","authors":"R. Kadek, J. Filípek, K. Mikulková, J. Illek","doi":"10.2754/avb202291020133","DOIUrl":"https://doi.org/10.2754/avb202291020133","url":null,"abstract":"The aim of this study was to determine and compare the plasma and erythrocyte (RBCs) levels of vitamin E in cows of 3 different categories (2–3 weeks antepartum, 1–3 weeks and 2.5–3 months of lactation) and 1-month-old calves on dairy nutrition. We were interested in the degree of correlation of vitamin E in plasma and RBCs within the individual categories. Blood on EDTA was collected from 61 cows and 12 calves. As a part of the haematological examination, we determined haematocrit (HCT) immediately after the collection. We determined vitamin E from plasma and RBCs by a standard HPLC method. We compared the results of vitamin E in plasma and RBCs and correlated them. The concentration of vitamin E in the plasma and RBCs was 6.98 and 3.45 μmol/l, respectively, in cows 2–3 weeks antepartum; 1–3 weeks of lactation it was 4.98 and 3.34; 2.5–3 months of lactation 11.76 and 2.80 μmol/l; and in the case of calves 12.07 and 6.29 μmol/l. Weak correlations were observed between vitamin E in plasma and the RBC concentrations in the antepartum category R2 = 0.2076; 1–3 weeks of lactation R2 = 0.0369; 2.5–3 months of lactation R2 = 0.2403 and calves on dairy nutrition R2 = 0.4628. Vitamin E concentrations in RBCs were shown to be more stable than in plasma, where the concentrations varied. It is possible that vitamin E in RBCs could tell us more about the longer-term reserves of vitamin E in the organism. The highest concentration of vitamin E in plasma and RBCs, as well as a stronger correlation was found in calves.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giedrė Vokietytė-Vilėniškė, Simona Nagreckienė, Iveta Duliebaitė, V. Žilaitis
A horse needs to move in different gaits and carry a rider during riding. Therefore, the equine back must be in a good functional state. Preventing back disorders is one of the keys to ensuring a horse’s health. This study aimed to assess cranial osteopathy therapy as a treatment and prevention method for preventing and reducing back pain. Thirty-two thoroughbred horses were categorised by their backs’ functional status (16 without back pain and 16 with back pain). The mechanical nociceptive threshold was determined before and after osteopathic treatment by an algometer (pain test FPX 100) with pressure points between T14-T15, T18-L1, and L5-L6. The data were analysed with a significance of P < 0.05. The study found that cranial osteopathic therapy raised the mechanical nociceptive threshold average in 83.3% measured points for horses without back pain and in 50% measured points for those with back pain. This study revealed that both horses without back pain and horses with back pain had a positive response to cranial osteopathic therapy, as evidenced by the increased nociceptive threshold limits, indicating that osteopathic therapy can be used as a primary or additional treatment method for back dysfunction.
{"title":"Effectiveness of cranial osteopathy therapy on nociception in equine back as evaluated by pressure algometry","authors":"Giedrė Vokietytė-Vilėniškė, Simona Nagreckienė, Iveta Duliebaitė, V. Žilaitis","doi":"10.2754/avb202291040347","DOIUrl":"https://doi.org/10.2754/avb202291040347","url":null,"abstract":"A horse needs to move in different gaits and carry a rider during riding. Therefore, the equine back must be in a good functional state. Preventing back disorders is one of the keys to ensuring a horse’s health. This study aimed to assess cranial osteopathy therapy as a treatment and prevention method for preventing and reducing back pain. Thirty-two thoroughbred horses were categorised by their backs’ functional status (16 without back pain and 16 with back pain). The mechanical nociceptive threshold was determined before and after osteopathic treatment by an algometer (pain test FPX 100) with pressure points between T14-T15, T18-L1, and L5-L6. The data were analysed with a significance of P < 0.05. The study found that cranial osteopathic therapy raised the mechanical nociceptive threshold average in 83.3% measured points for horses without back pain and in 50% measured points for those with back pain. This study revealed that both horses without back pain and horses with back pain had a positive response to cranial osteopathic therapy, as evidenced by the increased nociceptive threshold limits, indicating that osteopathic therapy can be used as a primary or additional treatment method for back dysfunction.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Margaréta Kulcsár, Michaela Karamanová, F. Novotny, Z. Vilhanová, M. Pettenello, C. Tóthová
The aim of this study was to offer an alternative therapy for gastric ulcers in horses as the interest in natural products is growing not only among practicioners but also horse owners. We compared the treatment of equine gastric squamous mucosa ulcers by omeprazol and by nutraceutical herbal mixture PREVUL (Dzsar Ltd., FitoCavallo, Hungary). A total of 36 horses, 18 geldings, 15 non-pregnant mares and 3 stallions were diagnosed with Equine Squamous Gastric Disease (ESGD) by gastroscopy. Horses were randomly divided into three groups. The first group was treated with 4 mg/kg omeprazole paste (Peptizole, Norbrook, United Kingdom) per os once daily on empty stomach for 28 days. The second group received 20 g of pulverized herbal mixture PREVUL twice daily in cracked oats for 28 days. The third experimental group received 2 mg/kg omeprazole per os once daily on empty stomach followed by 15 g of PREVUL mixed with cracked oats twice daily for 28 days. Ten healthy horses were used as control to evaluate the palatability and voluntary intake of PREVUL. Control gastroscopy was performed afted 30 days. The use of PREVUL alone P < 0.05 or in combination with omeprazole paste P < 0.01 appears to improve the healing of gastric ulcers in horses.
{"title":"Phytotherapy compared to omeprazol therapy in equine gastric ulcers","authors":"Margaréta Kulcsár, Michaela Karamanová, F. Novotny, Z. Vilhanová, M. Pettenello, C. Tóthová","doi":"10.2754/avb202291040309","DOIUrl":"https://doi.org/10.2754/avb202291040309","url":null,"abstract":"The aim of this study was to offer an alternative therapy for gastric ulcers in horses as the interest in natural products is growing not only among practicioners but also horse owners. We compared the treatment of equine gastric squamous mucosa ulcers by omeprazol and by nutraceutical herbal mixture PREVUL (Dzsar Ltd., FitoCavallo, Hungary). A total of 36 horses, 18 geldings, 15 non-pregnant mares and 3 stallions were diagnosed with Equine Squamous Gastric Disease (ESGD) by gastroscopy. Horses were randomly divided into three groups. The first group was treated with 4 mg/kg omeprazole paste (Peptizole, Norbrook, United Kingdom) per os once daily on empty stomach for 28 days. The second group received 20 g of pulverized herbal mixture PREVUL twice daily in cracked oats for 28 days. The third experimental group received 2 mg/kg omeprazole per os once daily on empty stomach followed by 15 g of PREVUL mixed with cracked oats twice daily for 28 days. Ten healthy horses were used as control to evaluate the palatability and voluntary intake of PREVUL. Control gastroscopy was performed afted 30 days. The use of PREVUL alone P < 0.05 or in combination with omeprazole paste P < 0.01 appears to improve the healing of gastric ulcers in horses.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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