G. Yilmazbas-Mecitoglu, A. Keskin, E. Karakaya-Bilen, U. Taşdemir, A. Gumen
The aim of this study was to determine the effects of the follicle stimulating hormone (FSH) supplemented in the Ovsynch protocol on pregnancy per timed artificial insemination (P/TAI) in cyclic dairy cows. All cows (n = 383) included in the study received the Ovsynch protocol. The cows in FSH3 (n = 92), FSH4 (n = 88), and FSH3&4 (n = 91) were administered 20 mg FSH on day 3, or day 4, or both on days 3 and 4 of the protocol, respectively, whereas the control group (n = 112) did not receive any FSH treatment. The mean follicle number at TAI and ovulation number after TAI were similar among the FSH groups. However, the mean follicular size was smaller (~14.9 mm, P = 0.02) in FSH4 compared to the other groups (~15.9 mm). Pregnancy/TAI of the FSH4 group on day 31 (59.1%) and day 62 (53.4%) was higher than those of other groups on day 31 (45.5% in control, 45.7% in FSH3, and 46.2% in FSH3&4) and day 62 (42.9% in control, 43.5% in FSH3, and 40.7% in FSH3&4), but the difference was non-significant. Likewise, FSH4 (62.7%) had more (P = 0.05) P/TAI than the other groups (48.6% in control, 47.7% in FSH3, and 49.4% in FSH3&4) in synchronized cows. The cows which responded to the first gonadotropin-releasing hormone (GnRH) of Ovsynch in the FSH4 group (64.8%) had higher (P < 0.02) P/AI rate than the responding control cows (44.6%). We concluded that application of FSH on day 4 in cyclic cows which were responsive to the Ovsynch protocol can yield higher pregnancy rates, but more studies are needed to better demonstrate this potential effect.
{"title":"The effect of supplementation of follicle-stimulating hormone (FSH) into the Ovsynch protocol to increase the pregnancy rate in cyclic lactating dairy cows","authors":"G. Yilmazbas-Mecitoglu, A. Keskin, E. Karakaya-Bilen, U. Taşdemir, A. Gumen","doi":"10.2754/avb202291020125","DOIUrl":"https://doi.org/10.2754/avb202291020125","url":null,"abstract":"The aim of this study was to determine the effects of the follicle stimulating hormone (FSH) supplemented in the Ovsynch protocol on pregnancy per timed artificial insemination (P/TAI) in cyclic dairy cows. All cows (n = 383) included in the study received the Ovsynch protocol. The cows in FSH3 (n = 92), FSH4 (n = 88), and FSH3&4 (n = 91) were administered 20 mg FSH on day 3, or day 4, or both on days 3 and 4 of the protocol, respectively, whereas the control group (n = 112) did not receive any FSH treatment. The mean follicle number at TAI and ovulation number after TAI were similar among the FSH groups. However, the mean follicular size was smaller (~14.9 mm, P = 0.02) in FSH4 compared to the other groups (~15.9 mm). Pregnancy/TAI of the FSH4 group on day 31 (59.1%) and day 62 (53.4%) was higher than those of other groups on day 31 (45.5% in control, 45.7% in FSH3, and 46.2% in FSH3&4) and day 62 (42.9% in control, 43.5% in FSH3, and 40.7% in FSH3&4), but the difference was non-significant. Likewise, FSH4 (62.7%) had more (P = 0.05) P/TAI than the other groups (48.6% in control, 47.7% in FSH3, and 49.4% in FSH3&4) in synchronized cows. The cows which responded to the first gonadotropin-releasing hormone (GnRH) of Ovsynch in the FSH4 group (64.8%) had higher (P < 0.02) P/AI rate than the responding control cows (44.6%). We concluded that application of FSH on day 4 in cyclic cows which were responsive to the Ovsynch protocol can yield higher pregnancy rates, but more studies are needed to better demonstrate this potential effect.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Lenz, Dominika Macháčová, P. Konecná, L. Fiala, M. Kyllar, F. Tichý
Tissue fixation is an essential step in the performance of ancillary studies, including immunohistochemistry. The aim of this study was to compare the effect of various fixatives and fixative times on immunohistochemistry (IHC) in bovine and porcine endometrium using progesterone receptors and SOX2 antibodies. Immunohistochemical staining with progesterone receptors and SOX2 was performed on tissue samples fixed in formalin, Antigenfix, Greenfix, Bouin’s solution and methacarn at 1.5, 8.5, 15.5, 29.5, 64.5, 189.5, 249.5, 309.5 and 369.5 days of fixation. Formalin and Antigenfix proved to be the best fixative for both short-term and long-term fixation for IHC. Bouin’s solution was partially applicable for short-term (24 h) fixation. Greenfix and methacarn were absolutely inappropriate fixatives for IHC (completely negative staining using methacarn). These results were obtained on an automated immunostainer using EDTA buffer pH 8.4 as the antigen retrieval solution. When the staining procedure was performed manually and citrate buffer with different pH values was used, weakly positive results were obtained with both progesterone receptors (pH 8 and 9) and SOX2 (pH 7.2); however, significantly lower staining quality was obtained using the methacarn fixative compared to aldehyde fixatives. The reported findings demonstrated the superiority of aldehyde fixatives (formalin and Antigenfix) over alcohol fixatives (methacarn) and fixatives combining both denaturing and cross-linking proteins (Greenfix and Bouin’s solution) for IHC. Antigen retrieval-IHC using EDTA buffer was found to be excellent for aldehyde fixatives, but proved to be completely unsuitable for methacarn, Greenfix, and Bouin’s solution.
{"title":"Effects of different fixatives over different fixation times, including Antigenfix, on immunohistochemical studies","authors":"J. Lenz, Dominika Macháčová, P. Konecná, L. Fiala, M. Kyllar, F. Tichý","doi":"10.2754/avb202291020179","DOIUrl":"https://doi.org/10.2754/avb202291020179","url":null,"abstract":"Tissue fixation is an essential step in the performance of ancillary studies, including immunohistochemistry. The aim of this study was to compare the effect of various fixatives and fixative times on immunohistochemistry (IHC) in bovine and porcine endometrium using progesterone receptors and SOX2 antibodies. Immunohistochemical staining with progesterone receptors and SOX2 was performed on tissue samples fixed in formalin, Antigenfix, Greenfix, Bouin’s solution and methacarn at 1.5, 8.5, 15.5, 29.5, 64.5, 189.5, 249.5, 309.5 and 369.5 days of fixation. Formalin and Antigenfix proved to be the best fixative for both short-term and long-term fixation for IHC. Bouin’s solution was partially applicable for short-term (24 h) fixation. Greenfix and methacarn were absolutely inappropriate fixatives for IHC (completely negative staining using methacarn). These results were obtained on an automated immunostainer using EDTA buffer pH 8.4 as the antigen retrieval solution. When the staining procedure was performed manually and citrate buffer with different pH values was used, weakly positive results were obtained with both progesterone receptors (pH 8 and 9) and SOX2 (pH 7.2); however, significantly lower staining quality was obtained using the methacarn fixative compared to aldehyde fixatives. The reported findings demonstrated the superiority of aldehyde fixatives (formalin and Antigenfix) over alcohol fixatives (methacarn) and fixatives combining both denaturing and cross-linking proteins (Greenfix and Bouin’s solution) for IHC. Antigen retrieval-IHC using EDTA buffer was found to be excellent for aldehyde fixatives, but proved to be completely unsuitable for methacarn, Greenfix, and Bouin’s solution.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ninčáková, V. Vecerek, L. Válková, E. Voslářová, Michael J. Kaluza, Veronika Zavřelová
The study focused on the comparison of health of cattle, pigs, sheep, goats, rabbits, poultry and ostriches slaughtered in slaughterhouses in the Czech Republic based on the occurrence of findings detected during a postmortem veterinary inspection in the period from 2010 to 2019. The level of health was expressed as the so-called PA index obtained by the ratio of the number of findings to the total number of observations (15) during the pathoanatomical examination in the slaughterhouse multiplied by one hundred. Mammals (cattle, pigs, sheep and goats) generally had a higher PA index than birds (domestic chickens, turkeys, ducks, geese, ostriches), with the exception of kids and rabbits. The highest PA index was found in cows (15.13) and piglets (12.18); whereas the lowest PA index was found in broiler chickens (0.102), rabbits, and geese (both 0.14). In poultry, the PA index values were below 1, with the exception of laying hens (PA index 2.165). A higher PA index was found in a group of adult animals (the PA index ranged from 2.17 to 15.13) and groups of young animals culled from farms (the PA index ranged from 10.79 to 12.18) than in fattened animals (the PA index ranged from 0.10 to 5.32). A comprehensive overview of the health condition of slaughtered animals enables the farmers, veterinarians, transporters and slaughterhouse operators to take appropriate and precisely targeted preventive measures, thereby increasing the animal welfare and health in the future while reducing the incidence of carcass damage.
{"title":"Health status of slaughtered animals as indicated by postmortem inspection at slaughterhouses","authors":"S. Ninčáková, V. Vecerek, L. Válková, E. Voslářová, Michael J. Kaluza, Veronika Zavřelová","doi":"10.2754/avb202291010099","DOIUrl":"https://doi.org/10.2754/avb202291010099","url":null,"abstract":"The study focused on the comparison of health of cattle, pigs, sheep, goats, rabbits, poultry and ostriches slaughtered in slaughterhouses in the Czech Republic based on the occurrence of findings detected during a postmortem veterinary inspection in the period from 2010 to 2019. The level of health was expressed as the so-called PA index obtained by the ratio of the number of findings to the total number of observations (15) during the pathoanatomical examination in the slaughterhouse multiplied by one hundred. Mammals (cattle, pigs, sheep and goats) generally had a higher PA index than birds (domestic chickens, turkeys, ducks, geese, ostriches), with the exception of kids and rabbits. The highest PA index was found in cows (15.13) and piglets (12.18); whereas the lowest PA index was found in broiler chickens (0.102), rabbits, and geese (both 0.14). In poultry, the PA index values were below 1, with the exception of laying hens (PA index 2.165). A higher PA index was found in a group of adult animals (the PA index ranged from 2.17 to 15.13) and groups of young animals culled from farms (the PA index ranged from 10.79 to 12.18) than in fattened animals (the PA index ranged from 0.10 to 5.32). A comprehensive overview of the health condition of slaughtered animals enables the farmers, veterinarians, transporters and slaughterhouse operators to take appropriate and precisely targeted preventive measures, thereby increasing the animal welfare and health in the future while reducing the incidence of carcass damage.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This case report describes nodular dermatofibrosis in an 11-year-old female dog of the German Shepherd breed. Previously, at the age of 6 years (initial stage), a sample from a tumorous nodule on her back was removed. Histological examination of the sample from this period showed hyperplasia of cells with a lobular structure. Immunohistochemistry staining demonstrated focal positivity to pancytokeratin. In the terminal stage (at 11 years of age), clinical examination revealed apathy, uncoordinated movement of the hind limbs, obstipation, anorexia and occasional vomiting with progressive weight loss. Skin inspection found multiple skin ulcerating tumorous lesions localized in the sacral region of the back and intercostally, partly fluctuating around the size 4 cm in diameter. Necropsy revealed an intraabdominal tumour localized among intestinal loops. Nodular lesions were found also in the lung parenchyma, on the dorsal surface of the epiglottis, in the myocardium, the cortex and the medulla of the kidneys, the adrenal gland, and in the intestinal wall. Histological analysis showed systemic production of fibrous nodules and formation of fibrous tissue with atrophy of parenchyma tissue. However, no connection between dermatofibrosis and adenocarcinoma of the kidney was found in this case, which was supplemented with pancytokeratin antibody. CD3 + lymphocytes were observed mainly in the zone of cell proliferation and in the interface towards the fibrous layer. Macrophages were seen mainly in the transitional zone between cellular and fibrous part. This indicated participation of monitored immunocompetent cells in fibroblast degradation.
{"title":"Immunohistochemistry of nodular dermatofibrosis in a German Shepherd – a case report","authors":"M. Levkut, M. Levkutová, M. Čechová","doi":"10.2754/avb202291030273","DOIUrl":"https://doi.org/10.2754/avb202291030273","url":null,"abstract":"This case report describes nodular dermatofibrosis in an 11-year-old female dog of the German Shepherd breed. Previously, at the age of 6 years (initial stage), a sample from a tumorous nodule on her back was removed. Histological examination of the sample from this period showed hyperplasia of cells with a lobular structure. Immunohistochemistry staining demonstrated focal positivity to pancytokeratin. In the terminal stage (at 11 years of age), clinical examination revealed apathy, uncoordinated movement of the hind limbs, obstipation, anorexia and occasional vomiting with progressive weight loss. Skin inspection found multiple skin ulcerating tumorous lesions localized in the sacral region of the back and intercostally, partly fluctuating around the size 4 cm in diameter. Necropsy revealed an intraabdominal tumour localized among intestinal loops. Nodular lesions were found also in the lung parenchyma, on the dorsal surface of the epiglottis, in the myocardium, the cortex and the medulla of the kidneys, the adrenal gland, and in the intestinal wall. Histological analysis showed systemic production of fibrous nodules and formation of fibrous tissue with atrophy of parenchyma tissue. However, no connection between dermatofibrosis and adenocarcinoma of the kidney was found in this case, which was supplemented with pancytokeratin antibody. CD3 + lymphocytes were observed mainly in the zone of cell proliferation and in the interface towards the fibrous layer. Macrophages were seen mainly in the transitional zone between cellular and fibrous part. This indicated participation of monitored immunocompetent cells in fibroblast degradation.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Necidová, Š. Bursová, Danka Haruštiaková, Kateřina Bogdanovičová
Staphylococcal food poisoning is one of the most prevalent causes of foodborne intoxication worldwide. Sandwiches and desserts are susceptible to contamination by S. aureus due to the high proportion of manual work during their production. Our study aimed to evaluate the impact of storage conditions on staphylococcal enterotoxin production in sandwiches and buttercream puffs. Foods were inoculated with different S. aureus strains capable of producing type A, B, and C staphylococcal enterotoxins and incubated at 15, 25, and 30 °C. During the storage, samples were analysed for S. aureus counts and for staphylococcal enterotoxins. An enzyme-linked fluorescence assay was used to detect staphylococcal enterotoxins. The influence of inappropriate storage on S. aureus growth and staphylococcal enterotoxin production was evaluated. No staphylococcal enterotoxins were detected in sandwiches stored for 72 h at any of the tested temperatures. In buttercream puffs, enterotoxins type A, B, and C were detected within 24 h of storage at 25 °C.
{"title":"Evaluation of Staphylococcus aureus growth and staphylococcal enterotoxin production in delicatessen and fine bakery products","authors":"L. Necidová, Š. Bursová, Danka Haruštiaková, Kateřina Bogdanovičová","doi":"10.2754/avb202291040417","DOIUrl":"https://doi.org/10.2754/avb202291040417","url":null,"abstract":"Staphylococcal food poisoning is one of the most prevalent causes of foodborne intoxication worldwide. Sandwiches and desserts are susceptible to contamination by S. aureus due to the high proportion of manual work during their production. Our study aimed to evaluate the impact of storage conditions on staphylococcal enterotoxin production in sandwiches and buttercream puffs. Foods were inoculated with different S. aureus strains capable of producing type A, B, and C staphylococcal enterotoxins and incubated at 15, 25, and 30 °C. During the storage, samples were analysed for S. aureus counts and for staphylococcal enterotoxins. An enzyme-linked fluorescence assay was used to detect staphylococcal enterotoxins. The influence of inappropriate storage on S. aureus growth and staphylococcal enterotoxin production was evaluated. No staphylococcal enterotoxins were detected in sandwiches stored for 72 h at any of the tested temperatures. In buttercream puffs, enterotoxins type A, B, and C were detected within 24 h of storage at 25 °C.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Kačírová, M. Sondorová, Natália Šurín Hudáková, T. Lipták, A. Maďari, K. Mravcová, Lívia Kolesár Fecskeová, R. Mucha, M. Maďar
Periodontal diseases are the most common condition in companion animal practice. The administration of antibiotics is associated with the therapy of these diseases. The aim of the present study was to verify the effect of antibiotics on canine dental biofilm bacteria due to increasing antibiotic resistance. Dental biofilm samples were taken from six dogs before and after administration of antibiotics, specifically, the combination of spiramycin and metronidazole. The samples were cultured on solid media under aerobic and anaerobic conditions. Sequencing analysis of the 16S rRNA gene was used to identify bacterial isolates. In addition, total bacterial DNA was extracted from samples from one dog and the V3−V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq platform. Using cultivation, 55 isolates belonging to 4 phyla were isolated before antibiotics administration, and 36 isolates belonging to 3 phyla were isolated after antibiotics administration. A significant decrease was noted in the genera Porphyromonas, Neisseria and Frederiksenia, whereas there was a significant increase in the genus Streptococcus. Of the total microbiota, there were 69 operational taxonomic units (OTUs) belonging to 11 phyla before antibiotics administration, and 51 OTUs belonging to 8 phyla after antibiotics administration. A significant decrease was recorded in the genus Porphyromonas, while a significant increase in the genus Capnocytophaga. The significant effect of spiramycin and metronidazole on the genus Porphyromonas at the time of their administration was confirmed by both cultivation and amplicon sequencing.
{"title":"Effect of spiramycin and metronidazole on canine dental biofilm bacteria","authors":"J. Kačírová, M. Sondorová, Natália Šurín Hudáková, T. Lipták, A. Maďari, K. Mravcová, Lívia Kolesár Fecskeová, R. Mucha, M. Maďar","doi":"10.2754/avb202291040375","DOIUrl":"https://doi.org/10.2754/avb202291040375","url":null,"abstract":"Periodontal diseases are the most common condition in companion animal practice. The administration of antibiotics is associated with the therapy of these diseases. The aim of the present study was to verify the effect of antibiotics on canine dental biofilm bacteria due to increasing antibiotic resistance. Dental biofilm samples were taken from six dogs before and after administration of antibiotics, specifically, the combination of spiramycin and metronidazole. The samples were cultured on solid media under aerobic and anaerobic conditions. Sequencing analysis of the 16S rRNA gene was used to identify bacterial isolates. In addition, total bacterial DNA was extracted from samples from one dog and the V3−V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq platform. Using cultivation, 55 isolates belonging to 4 phyla were isolated before antibiotics administration, and 36 isolates belonging to 3 phyla were isolated after antibiotics administration. A significant decrease was noted in the genera Porphyromonas, Neisseria and Frederiksenia, whereas there was a significant increase in the genus Streptococcus. Of the total microbiota, there were 69 operational taxonomic units (OTUs) belonging to 11 phyla before antibiotics administration, and 51 OTUs belonging to 8 phyla after antibiotics administration. A significant decrease was recorded in the genus Porphyromonas, while a significant increase in the genus Capnocytophaga. The significant effect of spiramycin and metronidazole on the genus Porphyromonas at the time of their administration was confirmed by both cultivation and amplicon sequencing.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"28 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Maďar, J. Kačírová, A. Maďari, R. Mucha, E. Styková, R. Nemcová
Dental diseases are a persistent problem, not only in humans, but very often in companion animals as well. Aetiological agents of these diseases are the dental plaque bacteria. In the present study, we focused on identifying cultivable bacteria living in the dental plaque of dogs, specifically dogs suffering from the early stages of periodontal disease. Canine oral bacteria pose a risk to humans that get bitten by the dog, but they also have a zoonotic potential. Dental plaque samples were taken from five dogs of small breeds. Samples were cultured under aerobic and anaerobic conditions on several types of microbiological agars. All obtained and selected bacterial colonies were identified by PCR with universal primers for the 16S rRNA gene and the sequences of the 16S rRNA gene were compared with the sequences available in the GenBank database using BLASTn analysis. A total of 75 bacteria belonging to five phyla, predominantly to Firmicutes and Proteobacteria, were identified. The most frequent species was Pasteurella canis which was detected in all samples. In addition, representatives of the genera Actinomyces, Bacillus, Bacteroides, Corynebacterium, Frederiksenia, Fusobacterium, Haemophilus, Lactobacillus, Leucobacter, Neisseria, Ottowia, Porphyromonas, Pseudomonas, Staphylococcus, Stenotrophomonas and Streptococcus were detected in the samples. In the present study, a broad spectrum of bacteria in dental plaque samples, including canine periodontal pathogens such as Porphyromonas gulae or Porphyromonas macacae were identified. In addition, highly pathogenic bacteria, specifically Actinomyces hordeovulneris, Bacillus circulans, and Bacteroides pyogenes, which pose a serious risk to human health, were detected in samples.
{"title":"Cultivable bacterial diversity of the canine dental plaque as a potential source of bacterial infections","authors":"M. Maďar, J. Kačírová, A. Maďari, R. Mucha, E. Styková, R. Nemcová","doi":"10.2754/avb202190020171","DOIUrl":"https://doi.org/10.2754/avb202190020171","url":null,"abstract":"Dental diseases are a persistent problem, not only in humans, but very often in companion animals as well. Aetiological agents of these diseases are the dental plaque bacteria. In the present study, we focused on identifying cultivable bacteria living in the dental plaque of dogs, specifically dogs suffering from the early stages of periodontal disease. Canine oral bacteria pose a risk to humans that get bitten by the dog, but they also have a zoonotic potential. Dental plaque samples were taken from five dogs of small breeds. Samples were cultured under aerobic and anaerobic conditions on several types of microbiological agars. All obtained and selected bacterial colonies were identified by PCR with universal primers for the 16S rRNA gene and the sequences of the 16S rRNA gene were compared with the sequences available in the GenBank database using BLASTn analysis. A total of 75 bacteria belonging to five phyla, predominantly to Firmicutes and Proteobacteria, were identified. The most frequent species was Pasteurella canis which was detected in all samples. In addition, representatives of the genera Actinomyces, Bacillus, Bacteroides, Corynebacterium, Frederiksenia, Fusobacterium, Haemophilus, Lactobacillus, Leucobacter, Neisseria, Ottowia, Porphyromonas, Pseudomonas, Staphylococcus, Stenotrophomonas and Streptococcus were detected in the samples. In the present study, a broad spectrum of bacteria in dental plaque samples, including canine periodontal pathogens such as Porphyromonas gulae or Porphyromonas macacae were identified. In addition, highly pathogenic bacteria, specifically Actinomyces hordeovulneris, Bacillus circulans, and Bacteroides pyogenes, which pose a serious risk to human health, were detected in samples.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44297475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angiomyolipoma is an extremely rare neoplasm in animals. It belongs to a group of perivascular epithelioid cell tumours (so-called PEComas). This study reports a case of primary soft tissue angiomyolipoma in a dog with some unusual clinical features. A 4-year old female Labrador dog with a rapidly growing pelvic tumour measuring 30 × 20 cm with a short history of 12 weeks was presented. The tumour was well-circumscribed and pushed into the right vaginal wall and into the perineum. The tumour was completely surgically excised. An extensive histological examination was performed, including immunohistochemical analysis. Histology revealed a mesenchymal neoplasm consisting of three tissue components – mature adipose tissue (which dominated), vessels, and smooth muscle (spindle) cells. The lesion showed positive immunohistochemical staining with smooth muscle actin and desmin in mature-appearing smooth muscle cells and S-100 protein positivity in adipocytes. The diagnosis of angiomyolipoma must be considered in different types of benign and malignant tumours of various lineages. The diagnostic approach to soft tissue tumours, including angiomyolipoma, requires optimal processing and sectioning of resected specimens. This paper is believed to be the first case of primary soft tissue angiomyolipoma reported in the veterinary literature.
{"title":"Primary soft tissue angiomyolipoma in a dog with unusual clinical features","authors":"J. Lenz, P. Konecná, F. Tichý, L. Fiala","doi":"10.2754/avb202190020179","DOIUrl":"https://doi.org/10.2754/avb202190020179","url":null,"abstract":"Angiomyolipoma is an extremely rare neoplasm in animals. It belongs to a group of perivascular epithelioid cell tumours (so-called PEComas). This study reports a case of primary soft tissue angiomyolipoma in a dog with some unusual clinical features. A 4-year old female Labrador dog with a rapidly growing pelvic tumour measuring 30 × 20 cm with a short history of 12 weeks was presented. The tumour was well-circumscribed and pushed into the right vaginal wall and into the perineum. The tumour was completely surgically excised. An extensive histological examination was performed, including immunohistochemical analysis. Histology revealed a mesenchymal neoplasm consisting of three tissue components – mature adipose tissue (which dominated), vessels, and smooth muscle (spindle) cells. The lesion showed positive immunohistochemical staining with smooth muscle actin and desmin in mature-appearing smooth muscle cells and S-100 protein positivity in adipocytes. The diagnosis of angiomyolipoma must be considered in different types of benign and malignant tumours of various lineages. The diagnostic approach to soft tissue tumours, including angiomyolipoma, requires optimal processing and sectioning of resected specimens. This paper is believed to be the first case of primary soft tissue angiomyolipoma reported in the veterinary literature.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41530345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Marino, C. Vullo, Stefania Di Giorgio, A. Zanghì, G. Catone, A. Sfacteria
This study aimed to investigate the morphological patterns of the genital tract after long-term treatment of deslorelin acetate in a female cat, a gonadotropin-releasing hormone agonist currently used in adult cats to obtain transient oestrus suppression. A 1-year-old Chartreux female cat was treated with 4.7 mg deslorelin acetate to suppress oestrus manifestations. The treatment was repeated for a total of × 3 every 2 years. After 8 years, the female cat came into oestrus again, but she was no more implanted, and an ovariohysterectomy was performed. Before surgery, an ultrasound examination was performed that showed a normal uterus and the presence of about 5 follicles in ovaries. Concentrations of oestradiol, progesterone, and vaginal smears were compatible with oestrus. During surgery, a very short ovarian pedicle was observed yet neither uterus nor ovaries presented appreciable alterations. At histology, the ovaries presented a juvenile appearance with numerous primordial and periovulatory follicles. The uterus showed marked endometrial hyperplasia with polypoid projection and atrophic myometrium. Based on this case report, deslorelin acetate is a powerful drug able to preserve ovarian function. However, the suppression of gonadotrophin, especially for a long period, has a detrimental atrophic effect on the target organs during treatment and, on the opposite, hyperplastic changes may occur after the restoring of normal cyclicity.
{"title":"Hyperplastic and atrophic changes in the genital tract of a female cat following repeated treatment with deslorelin acetate – a case report","authors":"G. Marino, C. Vullo, Stefania Di Giorgio, A. Zanghì, G. Catone, A. Sfacteria","doi":"10.2754/avb202190020207","DOIUrl":"https://doi.org/10.2754/avb202190020207","url":null,"abstract":"This study aimed to investigate the morphological patterns of the genital tract after long-term treatment of deslorelin acetate in a female cat, a gonadotropin-releasing hormone agonist currently used in adult cats to obtain transient oestrus suppression. A 1-year-old Chartreux female cat was treated with 4.7 mg deslorelin acetate to suppress oestrus manifestations. The treatment was repeated for a total of × 3 every 2 years. After 8 years, the female cat came into oestrus again, but she was no more implanted, and an ovariohysterectomy was performed. Before surgery, an ultrasound examination was performed that showed a normal uterus and the presence of about 5 follicles in ovaries. Concentrations of oestradiol, progesterone, and vaginal smears were compatible with oestrus. During surgery, a very short ovarian pedicle was observed yet neither uterus nor ovaries presented appreciable alterations. At histology, the ovaries presented a juvenile appearance with numerous primordial and periovulatory follicles. The uterus showed marked endometrial hyperplasia with polypoid projection and atrophic myometrium. Based on this case report, deslorelin acetate is a powerful drug able to preserve ovarian function. However, the suppression of gonadotrophin, especially for a long period, has a detrimental atrophic effect on the target organs during treatment and, on the opposite, hyperplastic changes may occur after the restoring of normal cyclicity.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49462421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Proximal interphalangeal joint (PIPJ) arthrodesis is performed commonly in horses with chronic osteoarthritis of the pastern joint or in cases of acute traumatic injury to the pastern, in which the weight-bearing bony column must be restored. Chronic osteoarthritis of the pastern joint is a frequent cause of lameness in the equine athlete and is evidenced by chronic lameness which is supported radiographically by periosteal proliferation and loss of joint space. The conventional method of joint fusion involves the opening of the joint, debridement of the joint cartilage, osteostixis of the subchondral bone plate and application of either lag screws or plate. This case report describes the successful treatment of chronic proliferative ossified osteoarthritis and periarthritis of the proximal interphalangeal joint of the front limb of two mares by performing simple percutaneous drilling destruction of the articular cartilage and stabilization with the application of three lag screws. After three years of conservative treatment with local corticosteroids, the mares exhibited non-weight-bearing lameness. Radiographs revealed extensive periarticular new bone formation over the proximal interphalangeal joint. Three 4.5 mm transcortical bone screws were placed in the lag fashion. A lower limb fiberglass cast was applied in both cases for 3 days. The antibiotic and analgesic protocol included gentamicin, penicillin, and flunixin meglumine for several days. Control radiographic examination after a period of 6 and 12 months showed reduction of the periarticular bone tissue. Both of the mares in this case study successfully returned to pleasure riding after undergoing forelimb arthrodesis.
{"title":"Proximal interphalangeal joint arthrodesis in a case of chronic proliferative osteoarthritis in the horse – a case report","authors":"F. Koľvek, Š. Krisová, K. Žuffová, Z. Žert","doi":"10.2754/avb202190020155","DOIUrl":"https://doi.org/10.2754/avb202190020155","url":null,"abstract":"Proximal interphalangeal joint (PIPJ) arthrodesis is performed commonly in horses with chronic osteoarthritis of the pastern joint or in cases of acute traumatic injury to the pastern, in which the weight-bearing bony column must be restored. Chronic osteoarthritis of the pastern joint is a frequent cause of lameness in the equine athlete and is evidenced by chronic lameness which is supported radiographically by periosteal proliferation and loss of joint space. The conventional method of joint fusion involves the opening of the joint, debridement of the joint cartilage, osteostixis of the subchondral bone plate and application of either lag screws or plate. This case report describes the successful treatment of chronic proliferative ossified osteoarthritis and periarthritis of the proximal interphalangeal joint of the front limb of two mares by performing simple percutaneous drilling destruction of the articular cartilage and stabilization with the application of three lag screws. After three years of conservative treatment with local corticosteroids, the mares exhibited non-weight-bearing lameness. Radiographs revealed extensive periarticular new bone formation over the proximal interphalangeal joint. Three 4.5 mm transcortical bone screws were placed in the lag fashion. A lower limb fiberglass cast was applied in both cases for 3 days. The antibiotic and analgesic protocol included gentamicin, penicillin, and flunixin meglumine for several days. Control radiographic examination after a period of 6 and 12 months showed reduction of the periarticular bone tissue. Both of the mares in this case study successfully returned to pleasure riding after undergoing forelimb arthrodesis.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2021-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44507655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：