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Assessing the anti-inflammatory effects of whole-body vibration: a meta-analysis based on pre-clinical and clinical evidences. 评估全身振动的抗炎效果:基于临床前和临床证据的荟萃分析。
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/LLGY4023
Yiying Wang, Renxin Ji, Bo Yu

Background: Whole-body vibration (WBV) is a commonly used physical exercise for disease prevention and rehabilitation. Recent studies indicated the beneficial mechanism of WBV may be associated with its anti-inflammatory potential, however, its regulatory roles on different inflammatory mediators remained controversial. The aim of this study was to perform a meta-analysis to re-confirm the effects of WBV exercise on various inflammatory factors.

Methods: The PubMed, EMBASE and Cochrane Library databases were searched up to September 2023 to collect all articles comparing WBV with control (or post-pre trials). The effect size was expressed as the standardized mean difference (SMD) and 95% confidence intervals (CI).

Results: A total of 31 eligible studies were included, including 14 pre-clinical and 17 clinical studies. The meta-analysis of pre-clinical studies showed that compared with the control group, WBV exercise could significantly reduce the level of IL-6 (SMD: -1.03, 95% CI: -1.93, -0.13), TNF-α (SMD: -1.36, 95% CI: -2.54, -0.17) (for disease subgroup), IL-1β (SMD: -2.20, 95% CI: -3.24, -1.15), IFN-γ (SMD: -1.91, 95% CI: -2.71, -1.12), IL-4 (SMD: -0.71, 95% CI: -1.39, -0.03) and IL-17 (SMD: -1.32, 95% CI: -2.05, -0.59) overall. Pooling of clinical studies revealed WBV exercise significantly reduced the level of TNF-α (WBV vs control: SMD: -1.11, 95% CI: -2.16, -0.06; post vs pre: SMD: -1.29, 95% CI: -1.91, -0.67), CRP (SMD: -3.59, 95% CI: -6.36, -0.82, P = 0.011) and enhanced the level of IL-10 (WBV vs control: SMD: 2.90, 95% CI: 1.10, 4.71; post vs pre: SMD: 1.75, 95% CI: 0.64, 2.87) and IL-6 (SMD: 0.91, 95% CI: 0.31, 1.52) (healthy subgroup).

Conclusion: WBV may be an effective prevention and rehabilitation tool for inflammatory diseases.

背景:全身振动(WBV)是一种常用的疾病预防和康复体育锻炼方法。最近的研究表明,全身振动的有益机制可能与它的抗炎潜力有关,但它对不同炎症介质的调节作用仍存在争议。本研究旨在进行一项荟萃分析,以重新确认 WBV 运动对各种炎症因子的影响:方法:检索 PubMed、EMBASE 和 Cochrane Library 数据库,收集截至 2023 年 9 月所有比较 WBV 与对照(或后-前试验)的文章。效应大小以标准化平均差异(SMD)和 95% 置信区间(CI)表示:结果:共纳入了 31 项符合条件的研究,包括 14 项临床前研究和 17 项临床研究。临床前研究的荟萃分析表明,与对照组相比,WBV 运动能显著降低 IL-6 (SMD:-1.03,95% CI:-1.93,-0.13)、TNF-α (SMD:-1.36,95% CI:-2.54,-0.17)(疾病亚组)、IL-1β(SMD:-2.20,95% CI:-3.24,-1.15)、IFN-γ(SMD:-1.91,95% CI:-2.71,-1.12)、IL-4(SMD:-0.71,95% CI:-1.39,-0.03)和 IL-17(SMD:-1.32,95% CI:-2.05,-0.59)。对临床研究进行汇总后发现,WBV 运动能显著降低 TNF-α 的水平(WBV 与对照组相比,SMD:-1.11,95% CI:-1.39,-0.03):SMD:-1.11,95% CI:-2.16,-0.06;后与前:SMD:-1.29,95% CI:-1.91,-0.67)、CRP(SMD:-3.59,95% CI:-6.36,-0.82,P = 0.011)的水平,并提高了 IL-10 的水平(WBV 与对照组相比,SMD:2.90,95% CI:-2.05,-0.59):SMD:2.90,95% CI:1.10,4.71;后与前:SMD:1.75,95% CI:0.64,2.87)和 IL-6(SMD:0.91,95% CI:0.31,1.52)(健康亚组):WBV可能是预防和康复炎症性疾病的有效工具。
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引用次数: 0
Plasma free cell RNA profiling for the prediction of preeclampsia. 用于预测先兆子痫的血浆游离细胞 RNA 图谱。
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/RGRU1280
Yuting Liang
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引用次数: 0
Cutaneous manifestations associated with COVID-19 infection at a university hospital in eastern China. 中国东部某大学医院与COVID-19感染相关的皮肤表现。
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/BDUC7952
Lingyi Lu, Lu Cao, Jing Zhang, Bingjiang Lin

Background: Coronavirus disease 2019 (COVID-19) affects different organ systems, including the skin. A retrospective analysis of skin manifestations in Chinese outpatient and inpatient settings is lacking. The study aims to analyze cutaneous manifestations in COVID-19 patients and the recurrence or aggravation of previous skin diseases.

Materials and methods: A retrospective cross-sectional study was conducted from November 2022 to July 2023 in a university hospital in eastern China. It involved reverse transcriptase polymerase chain reaction (RT-PCR)-positive COVID-19 patients, documenting various skin manifestations and the recurrence or aggravation of pre-existing skin conditions. The pattern of skin lesions and other variables were assessed.

Results: The study included 303 patients, with 127 males and 176 females. Maculopapular rash was the predominant new cutaneous manifestation (54.92%), mainly in middle-aged individuals. Other findings included urticaria (16.39%), herpes zoster (11.89%), and herpes simplex (4.10%), vesicular rashes (2.46%), purpura (2.05%), erythema multiforme (1.64%), livedo reticularis (0.41%) and so on. Severe disease was associated with herpes zoster and livedo reticularis. Critical COVID-19 cases were linked to vesicular rashes, purpura, and erythema multiforme. The mean time for skin lesion emergence post-infection varied from 3 days for seborrheic dermatitis to 17.48 days for herpes zoster. Vasculitic manifestations correlated with elevated D-dimer levels. A total of 59 cases (19.47%) of recurrent or aggravated skin diseases were reported following infection with COVID-19, with dermatitis being the most common, followed by acne and folliculitis, psoriasis, urticaria, bullous pemphigoid, pemphigus, tinea corporis and androgenetic alopecia.

Conclusion: The cutaneous phenotypes delineated in this study expand the dermatologic spectrum associated with COVID-19. Cutaneous manifestations may result from overactive immune responses, complement activation, and microvascular damage. Herpes zoster typically occurs in elderly COVID-19 patients with weaker immune systems or more severe diseases. Purpura and livedo reticularis, although rare, may indicate disease severity. It is possible to predict the course of COVID-19 with different severity through cutaneous manifestations. Recognizing these skin manifestations could aid in predicting COVID-19 severity and guide dermatologists in managing the pandemic response.

背景:冠状病毒病2019(COVID-19)影响不同的器官系统,包括皮肤。目前尚缺乏对中国门诊和住院患者皮肤表现的回顾性分析。本研究旨在分析COVID-19患者的皮肤表现以及既往皮肤病的复发或加重情况:一项回顾性横断面研究于 2022 年 11 月至 2023 年 7 月在华东某大学附属医院进行。研究涉及逆转录酶聚合酶链反应(RT-PCR)阳性的 COVID-19 患者,记录了患者的各种皮肤表现以及既往皮肤病的复发或加重情况。对皮损模式和其他变量进行了评估:研究共纳入 303 名患者,其中男性 127 人,女性 176 人。斑丘疹是最主要的新皮肤表现(54.92%),主要发生在中年人身上。其他表现还包括荨麻疹(16.39%)、带状疱疹(11.89%)、单纯疱疹(4.10%)、水泡疹(2.46%)、紫癜(2.05%)、多形性红斑(1.64%)、网状青斑(0.41%)等。重症病例与带状疱疹和筋膜炎有关。COVID-19 重症病例与水泡性皮疹、紫癜和多形性红斑有关。感染后出现皮损的平均时间从3天(脂溢性皮炎)到17.48天(带状疱疹)不等。血管炎表现与 D-二聚体水平升高有关。感染 COVID-19 后,共有 59 例(19.47%)皮肤病复发或加重,其中皮炎最为常见,其次是痤疮和毛囊炎、银屑病、荨麻疹、大丘疹性类风湿、丘疹性荨麻疹、体癣和雄激素性脱发:本研究中描述的皮肤表型扩大了与 COVID-19 相关的皮肤病范围。皮肤表现可能源于过度活跃的免疫反应、补体激活和微血管损伤。带状疱疹通常发生在免疫系统较弱或疾病较严重的 COVID-19 老年患者身上。紫癜和网状青斑虽然罕见,但可表明疾病的严重程度。通过皮肤表现可以预测不同严重程度的 COVID-19 病程。识别这些皮肤表现有助于预测 COVID-19 的严重程度,并指导皮肤科医生管理大流行反应。
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引用次数: 0
Analysis of immune cells and risk factors related to lower limb deep vein thrombosis in patients with cerebral infarction. 脑梗塞患者下肢深静脉血栓形成的免疫细胞及相关风险因素分析。
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/DRPN1199
Feng-Dan Hu, Yun Miao, Bo Yu, Xiao-Zhen Deng, Ran Sun, Jin Qian, Hai-Xin Yuan

To explore the characteristics of hematologic indicators and related risk factors of lower extremity deep vein thrombosis (LDVT) in patients with cerebral infarction.

Methods: This study retrospectively analyzed data from 174 patients with cerebral infarction admitted to The Rehabilitation Department of Shanghai Fifth Rehabilitation Hospital and Shanghai First People's Hospital from June 2022 to June 2023. Based on the results of lower limb venous color Doppler ultrasound examinations, patients were divided into two groups: the LDVT group (35 cases) and the non-LDVT group (139 cases). We compared the clinical data and hematologic indicators (D-dimer value, fibrinogen, white blood cells, platelets, uric acid, creatinine, etc.) of the two groups to identify the risk factors of cerebral infarction complicated with LDVT.

Results: Statistical analysis revealed that the D-dimer values of the LDVT group were significantly (P<0.05) higher than those of the non-LDVT group. The uric acid value of the LDVT group was significantly lower than that of the non-LDVT group, with statistical significance (P<0.05). The Brunnstrom staging in the LDVT group was significantly different from that in the non-LDVT group (P<0.05). Meanwhile, binary logistic regression analysis showed that LDVT complicated with cerebral infarction was associated with D-dimer level [OR=1.302, 95% CI (1.077, 1.575)], uric acid level [OR=0.995, 95% CI (0.990, 1.000)], and Brunnstrom staging [OR=3.005, 95% CI (1.312, 6.880)].

Conclusion: D-dimer value, uric acid value, and Brunnstrom stage I to II are closely related to the occurrence of LDVT in patients with cerebral infarction. High D-dimer value, low uric acid value, and Brunnstrom stage I to II are independent risk factors for LDVT in cerebral infarction. Early assessment of D-dimer value, uric acid value, and Brunnstrom stage of cerebral infarction should be considered in clinical practice.

摘要】 目的 探讨脑梗死患者下肢深静脉血栓形成(LDVT)的血液学指标特点及相关危险因素:本研究回顾性分析了2022年6月至2023年6月期间上海市第五康复医院康复科和上海市第一人民医院收治的174例脑梗死患者的资料。根据下肢静脉彩色多普勒超声检查结果,将患者分为两组:LDVT组(35例)和非LDVT组(139例)。我们比较了两组患者的临床资料和血液学指标(D-二聚体值、纤维蛋白原、白细胞、血小板、尿酸、肌酐等),以确定LDVT并发脑梗死的危险因素:统计分析表明,LDVT 组的 D-二聚体值显著(PConclusion:D-二聚体值、尿酸值、BrunnstromⅠ~Ⅱ期与脑梗死患者LDVT的发生密切相关。高 D-二聚体值、低尿酸值和 Brunnstrom I 至 II 期是脑梗死 LDVT 的独立危险因素。临床实践中应考虑对脑梗死的 D-二聚体值、尿酸值和 Brunnstrom 分期进行早期评估。
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引用次数: 0
Prognostic significance of LRPPRC and its association with immune infiltration in liver hepatocellular carcinoma. 肝肝细胞癌中 LRPPRC 的预后意义及其与免疫浸润的关系。
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/XTLJ1335
Yanqiu Zhang, Bin Feng, Yuting Liang, Qingqin Tang, Sheng Zhang, Zheng Zhang, Li Xu, Jingping Yin

Background: Leucine rich pentatricopeptide repeat containing (LRPPRC) protein is a multifunctional protein involved in cell cycle progression and tumor development. However, its prognostic significance and association with immune infiltration in Liver hepatocellular carcinoma (LIHC) remain unclear.

Methods: We utilized transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases of LIHC patients to investigate the potential pro-cancer role of LRPPRC, including differential expression of LRPPRC in LIHC, prognostic value, clinicopathological features, immune cell infiltration relevance and function enrichment analysis.

Results: Our findings suggest that LRPPRC is upregulated in LIHC and exhibits correlations with survival, clinical stage, and tumor grade in LIHC patients. Additionally, immune infiltration analysis revealed significant negative correlations between LRPPRC expression and multiple tumor-infiltrating immune cells, including CTLs, DCs, pDCs, B cells, Th17 cells, neutrophils, T cells, Mast cells, Th1 cells, Tregs, and NK cells, whereas a significant positive correlation was observed with infiltration of Th2 cells, T helper cells and Tcms. Furthermore, functional enrichment analysis indicated that LRPPRC may be involved in G2m checkpoint, mitotic spindle, E2f targets, Wnt Beta catenin signaling, spermatogenesis and other processes.

背景:富亮氨酸五肽重复蛋白(LRPPRC)是一种参与细胞周期进展和肿瘤发生的多功能蛋白。然而,它在肝肝细胞癌(LIHC)中的预后意义及其与免疫浸润的关系仍不清楚:我们利用癌症基因组图谱(TCGA)和基因型组织表达(GTEx)数据库中LIHC患者的转录组和临床数据研究了LRPPRC的潜在促癌作用,包括LRPPRC在LIHC中的差异表达、预后价值、临床病理特征、免疫细胞浸润相关性和功能富集分析:我们的研究结果表明,LRPPRC在LIHC中上调,并与LIHC患者的生存期、临床分期和肿瘤分级相关。此外,免疫浸润分析表明,LRPPRC 的表达与多种肿瘤浸润免疫细胞(包括 CTLs、DCs、pDCs、B 细胞、Th17 细胞、中性粒细胞、T 细胞、肥大细胞、Th1 细胞、Tregs 和 NK 细胞)呈显著负相关,而与 Th2 细胞、T 辅助细胞和 Tcms 的浸润呈显著正相关。此外,功能富集分析表明,LRPPRC 可能参与了 G2m 检查点、有丝分裂纺锤体、E2f 靶点、Wnt Beta 连环素信号转导、精子发生等过程。
{"title":"Prognostic significance of LRPPRC and its association with immune infiltration in liver hepatocellular carcinoma.","authors":"Yanqiu Zhang, Bin Feng, Yuting Liang, Qingqin Tang, Sheng Zhang, Zheng Zhang, Li Xu, Jingping Yin","doi":"10.62347/XTLJ1335","DOIUrl":"10.62347/XTLJ1335","url":null,"abstract":"<p><strong>Background: </strong>Leucine rich pentatricopeptide repeat containing (LRPPRC) protein is a multifunctional protein involved in cell cycle progression and tumor development. However, its prognostic significance and association with immune infiltration in Liver hepatocellular carcinoma (LIHC) remain unclear.</p><p><strong>Methods: </strong>We utilized transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases of LIHC patients to investigate the potential pro-cancer role of LRPPRC, including differential expression of LRPPRC in LIHC, prognostic value, clinicopathological features, immune cell infiltration relevance and function enrichment analysis.</p><p><strong>Results: </strong>Our findings suggest that LRPPRC is upregulated in LIHC and exhibits correlations with survival, clinical stage, and tumor grade in LIHC patients. Additionally, immune infiltration analysis revealed significant negative correlations between LRPPRC expression and multiple tumor-infiltrating immune cells, including CTLs, DCs, pDCs, B cells, Th17 cells, neutrophils, T cells, Mast cells, Th1 cells, Tregs, and NK cells, whereas a significant positive correlation was observed with infiltration of Th2 cells, T helper cells and Tcms. Furthermore, functional enrichment analysis indicated that LRPPRC may be involved in G2m checkpoint, mitotic spindle, E2f targets, Wnt Beta catenin signaling, spermatogenesis and other processes.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 3","pages":"105-116"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of macrophage-related genes in bladder cancer patients using single-cell sequencing and construction of a prognostic model. 利用单细胞测序鉴定膀胱癌患者的巨噬细胞相关基因并构建预后模型
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/VLDZ7581
Weizhuo Wang, Junheng Shen, Dalong Song, Kai Fu, Xu Fu

Single-cell sequencing is an emerging technology that can effectively identify cell types in tumors. In the tumor microenvironment of bladder cancer, macrophages play a crucial role in invasion and immune escape. This study aimed to assess the expression of macrophage-related genes (MRGs) in the tumor microenvironment of bladder cancer patients and construct a prognostic model based on MRGs using bioinformatics methods.

Methods: Single-cell sequencing data from bladder cancer patients was downloaded from the GEO. After quality control and cell type identification, macrophages in the samples were extracted for re-clustering. Feature genes were then identified, and MRGs were assessed. Genetic data from TCGA database bladder cancer patients was also downloaded and organized. The intersection of MRGs and the TCGA gene set was determined. Clinical information was connected with this intersection, and the data was divided into training and validation sets. The training set was used for model construction and the validation set for model verification. A prognostic model based on MRGs was built using the LASSO algorithm and Cox regression. Patients were divided into high-risk and low-risk groups based on their prognostic features, and survival information in the training and validation sets was observed. The predictive ability of the model was assessed using a ROC curve, followed by a calibration plot to predict 1-, 3-, and 5-year survival rates.

Results: Four cell types were identified, and after extracting macrophages, three cell subgroups were clustered, resulting in 1,078 feature genes. The top 100 feature genes from each macrophage subgroup were extracted and intersected with TCGA expressed genes to construct the model. A risk prediction model composed of CD74, METRN, PTPRR, and CDC42EP5 was obtained. The survival and ROC curves showed that this model had good predictive ability. A calibration curve also demonstrated good prognostic ability for patients.

Conclusion: This study, based on single-cell data, TCGA data, and clinical information, constructed an MRG-based prognostic model for bladder cancer using multi-omics methods. This model has good accuracy and reliability in predicting the survival and prognosis of patients with bladder cancer, providing a reference for understanding the interaction between MRGs and bladder cancer.

单细胞测序是一种新兴技术,能有效识别肿瘤中的细胞类型。在膀胱癌的肿瘤微环境中,巨噬细胞对肿瘤的侵袭和免疫逃逸起着至关重要的作用。本研究旨在评估膀胱癌患者肿瘤微环境中巨噬细胞相关基因(MRGs)的表达情况,并利用生物信息学方法构建基于MRGs的预后模型:方法:从 GEO 下载膀胱癌患者的单细胞测序数据。方法:从 GEO 下载膀胱癌患者的单细胞测序数据,经过质量控制和细胞类型鉴定后,提取样本中的巨噬细胞进行重新聚类。然后确定特征基因,评估MRGs。此外,还从 TCGA 数据库下载并整理了膀胱癌患者的基因数据。确定MRGs与TCGA基因集的交集。临床信息与该交集相连,数据被分为训练集和验证集。训练集用于构建模型,验证集用于验证模型。利用 LASSO 算法和 Cox 回归建立了基于 MRGs 的预后模型。根据预后特征将患者分为高风险组和低风险组,并观察训练集和验证集的生存信息。使用 ROC 曲线评估了模型的预测能力,随后使用校准图预测了 1 年、3 年和 5 年的生存率:结果:确定了四种细胞类型,提取巨噬细胞后,对三个细胞亚群进行了聚类,得出了 1,078 个特征基因。从每个巨噬细胞亚群中提取前100个特征基因,并与TCGA表达基因交叉构建模型。得到了一个由CD74、METRN、PTPRR和CDC42EP5组成的风险预测模型。生存曲线和 ROC 曲线显示,该模型具有良好的预测能力。校准曲线也证明了该模型对患者具有良好的预后能力:本研究基于单细胞数据、TCGA 数据和临床信息,利用多组学方法构建了基于 MRG 的膀胱癌预后模型。该模型在预测膀胱癌患者的生存期和预后方面具有良好的准确性和可靠性,为了解MRGs与膀胱癌之间的相互作用提供了参考。
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引用次数: 0
Insight into NSCLC through novel analysis of gene interactions and characteristics. 通过对基因相互作用和特征的新分析,深入了解 NSCLC。
Q4 IMMUNOLOGY Pub Date : 2024-04-25 eCollection Date: 2024-01-01 DOI: 10.62347/ANLV4963
Eric Pan, Yongsheng Bai

Around 80 to 85% of all lung cancers are non-small cell lung cancer (NSCLC). Previous research has aimed at exploring the genetic basis of NSCLC through individual approaches, but studies have yet to investigate the results of combining them. Here we show that analyzing NSCLC genetics through three approaches simultaneously creates unique insights into our understanding of the disease. Through a combination of previous research and bioinformatics tools, we determined 35 NSCLC candidate genes. We analyzed these genes in 3 different approaches. First, we found the gene fusions between these candidate genes. Second, we found the common superfamilies between genes. Finally, we identified mutational signatures that are possibly associated with NSCLC. Each approach has its individual, unique results. Fusion relationships identify specific gene fusion targets, common superfamilies identify possible avenues to determine novel target genes, and identifying NSCLC associated mutational signatures has diagnostic and prognostic benefits. Combining the approaches, we found that gene CD74 has significant fusion relationships, but it has no association with the other two approaches, suggesting that CD74 is associated with NSCLC mainly because of its fusion relationships. Targeting the gene fusions of CD74 may be an alternative NSCLC treatment. This genetic analysis has indeed created unique insight into NSCLC genes. Both the results from each of the approaches separately and combined allow pursuit of more effective treatment strategies for this cancer. The methodology presented can also apply to other cancers, creating insights that current analytical methods could not find.

大约 80% 至 85% 的肺癌属于非小细胞肺癌(NSCLC)。以往的研究旨在通过单独的方法探索非小细胞肺癌的遗传基础,但尚未研究将这些方法结合起来的结果。在这里,我们展示了通过三种方法同时分析 NSCLC 遗传学能为我们了解这种疾病提供独特的见解。通过结合以往的研究和生物信息学工具,我们确定了 35 个 NSCLC 候选基因。我们用三种不同的方法对这些基因进行了分析。首先,我们发现了这些候选基因之间的基因融合。其次,我们发现了基因之间的共同超家族。最后,我们确定了可能与 NSCLC 相关的突变特征。每种方法都有其各自独特的结果。融合关系确定了特定的基因融合靶点,共同超家族确定了确定新靶基因的可能途径,而确定与 NSCLC 相关的突变特征则具有诊断和预后方面的益处。综合上述方法,我们发现 CD74 基因有显著的融合关系,但与其他两种方法没有关联,这表明 CD74 与 NSCLC 相关主要是因为其融合关系。针对 CD74 的基因融合可能是治疗 NSCLC 的另一种方法。这项基因分析确实对 NSCLC 基因有了独特的见解。无论是将每种方法的结果分开还是结合起来,都可以为这种癌症寻求更有效的治疗策略。所介绍的方法也可应用于其他癌症,从而提出目前的分析方法无法发现的见解。
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引用次数: 0
IFN-γ, IL-17, IL-22+ CD4+ subset in patients with hepatitis C virus and correlation with clinical factor. 丙型肝炎病毒感染者的 IFN-γ、IL-17、IL-22+ CD4+ 亚群及其与临床因素的相关性。
Q4 IMMUNOLOGY Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Soolmaz Khansalar, Zahra Faghih, Shaghik Barani, Mehdi Kalani, Mohammad Reza Ataollahi, Zeinab Mohammadi, Sepideh Namdari, Kurosh Kalantar

Background: CD4+ T cell responses in HCV infection have a crucial role in the immunopathology of hepatitis C virus (HCV) infection. Our aim was to investigate the frequency of Th1, Th17, and Th22 cells in HCV-infected patients and elucidate their role in the progression of the disease.

Methods: Twenty-six HCV-infected patients and 26 healthy individuals were recruited. Peripheral blood mononuclear cells (PBMCs) were stained to separate CD4, IFN-γ, IL-17, and IL-22 producing cells using flow cytometry.

Results: Results showed that the mean expression of IL-22 in CD4+ T cells was significantly lower in HCV-infected patients compared to healthy controls. About correlation with clinical factor and T subsets, a negative correlation between the frequency of CD4+ IFN-γ+ cells and Thyroxine level (T4) was observed in the patients. The data showed a positive link between thyroid-stimulating hormone (TSH), cholesterol levels, and the frequency of Th17 cells. In addition, a positive correlation was seen between serum creatinine level with both Th1 and Th17. Ultimately, it was found that there was a positive link between viral burden and IL-17+ IL-22+ cells and a negative correlation between viral load and pure Th22.

Conclusions: Our findings indicate that Th22 cells may play a part in the immunopathology of HCV and show the associations between Thelper subsets and the clinical signs of the disease.

背景:HCV感染中的CD4+T细胞反应在丙型肝炎病毒(HCV)感染的免疫病理学中起着至关重要的作用。我们的目的是调查 HCV 感染者体内 Th1、Th17 和 Th22 细胞的频率,并阐明它们在疾病进展中的作用:方法:我们招募了 26 名 HCV 感染者和 26 名健康人。采用流式细胞术对外周血单核细胞(PBMCs)进行染色,以分离产生 CD4、IFN-γ、IL-17 和 IL-22 的细胞:结果显示:与健康对照组相比,HCV 感染者 CD4+ T 细胞中 IL-22 的平均表达量明显较低。关于与临床因素和 T 亚群的相关性,在患者中观察到 CD4+ IFN-γ+ 细胞的频率与甲状腺素水平(T4)呈负相关。数据显示,促甲状腺激素(TSH)、胆固醇水平与 Th17 细胞的频率呈正相关。此外,血清肌酐水平与 Th1 和 Th17 细胞之间也存在正相关。最后,研究发现病毒负荷与 IL-17+ IL-22+ 细胞之间存在正相关,而病毒负荷与纯 Th22 细胞之间存在负相关:我们的研究结果表明,Th22细胞可能在HCV的免疫病理中起一定作用,并显示了Thlper亚群与疾病临床症状之间的关联。
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引用次数: 0
Exploring the role of NAA40 in immune infiltrates and prognostic prediction in hepatocellular carcinoma. 探索 NAA40 在肝细胞癌免疫浸润和预后预测中的作用。
Q4 IMMUNOLOGY Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Tong Zhou, Jun Cao, Qingqin Tang, Jieyu Jin, Yuting Liang, Bin Feng

NAA40 belongs to the N-terminal acetyltransferase (NATs) family, responsible for protein N-terminal modification, and it exerts crucial roles across various cancers. However, its impact on patient prognosis and immune infiltration in hepatocellular carcinoma (HCC) remains elusive. To address this, our study delved into the comprehensive analysis of NAA40 in the context of cancer. Our pan-cancer analysis unveiled elevated NAA40 expression in multiple tumor types, including BLCA, BRCA, CHOL, COAD, ESCA, HNSC, LIHC, LUAD, LUSC, STAD, and THCA. Additionally, through a comprehensive examination across various cancer types within TCGA, we discovered that high NAA40 gene expression correlated with poor prognosis in HCC, pointing toward its role in promoting oncogenesis. Further investigation illuminated the association of increased NAA40 expression with T stage, pathologic stage, tumor status, and histologic grade. Interestingly, we noted a significant inverse correlation between NAA40 expression and the infiltration levels of immune cells, such as DC cells, neutrophils, NK cells, and T cells, in liver cancer. This observation underpins the hypothesis that NAA40 influences HCC development by modulating immune cell infiltration. Functional enrichment analysis provided valuable insights into the pathways influenced by NAA40. Enriched pathways encompassed oxidative phosphorylation, xenobiotic metabolism, bile acid metabolism, fatty acid metabolism, G2M checkpoint, and E2F targets. These findings collectively position NAA40 as a potential biomarker for prognostic prediction and monitoring the effects of immunotherapy in HCC.

NAA40属于N端乙酰转移酶(NATs)家族,负责蛋白质的N端修饰,在各种癌症中发挥着重要作用。然而,它对肝细胞癌(HCC)患者预后和免疫浸润的影响仍然难以捉摸。为了解决这个问题,我们的研究深入研究了癌症背景下 NAA40 的全面分析。我们的泛癌症分析揭示了NAA40在多种肿瘤类型中的高表达,包括BLCA、BRCA、CHOL、COAD、ESCA、HNSC、LIHC、LUAD、LUSC、STAD和THCA。此外,通过对TCGA中各种癌症类型的全面检查,我们发现NAA40基因的高表达与HCC的不良预后相关,这表明NAA40在促进肿瘤发生中的作用。进一步的研究表明,NAA40表达的增加与T分期、病理分期、肿瘤状态和组织学分级有关。有趣的是,我们注意到NAA40的表达与肝癌中免疫细胞(如DC细胞、中性粒细胞、NK细胞和T细胞)的浸润水平呈显著的反相关。这一观察结果支持了NAA40通过调节免疫细胞浸润影响HCC发展的假设。功能富集分析为了解受NAA40影响的通路提供了宝贵的信息。富集途径包括氧化磷酸化、异生物代谢、胆汁酸代谢、脂肪酸代谢、G2M检查点和E2F靶点。这些发现共同将NAA40定位为一种潜在的生物标记物,用于预测HCC的预后和监测免疫疗法的效果。
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引用次数: 0
Identification of glycosyltransferase-related genes signature and integrative analyses in patients with ovarian cancer. 鉴定卵巢癌患者的糖基转移酶相关基因特征并进行综合分析。
Q4 IMMUNOLOGY Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Yanqiu Zhang, Tong Zhou, Qingqin Tang, Bin Feng, Yuting Liang

Background: Glycosyltransferases (GT) play a crucial role in glycosylation reactions, and aberrant expression of glycosyltransferase-related genes (GTs) leads to abnormal glycosylation, which is associated with tumor progression. However, the prognostic value of aberrant expression of GTs in ovarian cancer (OC) and the correlation between GTs and tumor microenvironment (TME) remain unknown.

Methods: TCGA and GSE53963 databases were used to obtain data on OC patient samples. The association of GTs with OC was analyzed. Molecular subtypes were identified by consensus unsupervised clustering, followed by immune infiltration and functional enrichment analyses. Survival analysis was performed using Kaplan-Meier curves and log-rank tests. Least Absolute Shrinkage and Selection Operator (LASSO) and multifactorial cox regression were used to screen for signature genes associated with OC and used to establish prognostic models.

Result: OC patients were categorized into 5 GTs clusters using consensus unsupervised cluster analysis. Clusters D and E showed significant differences between survival, signaling pathways and immune infiltration. Then, a risk model was developed based on the 12 signature genes, which provides a more accurate evaluation of the prognosis of OC patients. We categorized patients into high-risk and low-risk groups based on the risk score and found that the survival of patients in the high-risk group was significantly lower than that in the low-risk group. Moreover, the risk score was significantly correlated with tumor microenvironment, immune infiltration, and chemotherapy sensitivity.

Conclusion: Overall, we performed a comprehensive analysis of GTs in OC patients and developed a risk model for OC. Our findings will provide a new insight to OC prognosis and treatment.

背景:糖基转移酶(GT)在糖基化反应中起着至关重要的作用,糖基转移酶相关基因(GTs)的异常表达会导致糖基化异常,而糖基化异常与肿瘤进展有关。然而,GTs异常表达在卵巢癌(OC)中的预后价值以及GTs与肿瘤微环境(TME)之间的相关性仍然未知:方法:利用TCGA和GSE53963数据库获取OC患者样本数据。方法:利用 TCGA 和 GSE53963 数据库获取 OC 患者样本数据,分析 GTs 与 OC 的关联。通过共识无监督聚类确定分子亚型,然后进行免疫浸润和功能富集分析。利用卡普兰-梅耶曲线和对数秩检验进行了生存分析。利用最小绝对收缩和选择操作器(LASSO)和多因素cox回归筛选与OC相关的特征基因,并用于建立预后模型:结果:采用共识无监督聚类分析将OC患者分为5个GTs群。聚类D和E在生存、信号通路和免疫浸润方面存在显著差异。然后,根据这12个特征基因建立了一个风险模型,该模型能更准确地评估OC患者的预后。我们根据风险评分将患者分为高风险组和低风险组,发现高风险组患者的生存率明显低于低风险组。此外,风险评分与肿瘤微环境、免疫浸润和化疗敏感性有明显相关性:总之,我们对OC患者的GT进行了全面分析,并建立了OC的风险模型。总之,我们对 OC 患者的 GTs 进行了全面分析,并建立了 OC 的风险模型,我们的研究结果将为 OC 的预后和治疗提供新的见解。
{"title":"Identification of glycosyltransferase-related genes signature and integrative analyses in patients with ovarian cancer.","authors":"Yanqiu Zhang, Tong Zhou, Qingqin Tang, Bin Feng, Yuting Liang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Glycosyltransferases (GT) play a crucial role in glycosylation reactions, and aberrant expression of glycosyltransferase-related genes (GTs) leads to abnormal glycosylation, which is associated with tumor progression. However, the prognostic value of aberrant expression of GTs in ovarian cancer (OC) and the correlation between GTs and tumor microenvironment (TME) remain unknown.</p><p><strong>Methods: </strong>TCGA and GSE53963 databases were used to obtain data on OC patient samples. The association of GTs with OC was analyzed. Molecular subtypes were identified by consensus unsupervised clustering, followed by immune infiltration and functional enrichment analyses. Survival analysis was performed using Kaplan-Meier curves and log-rank tests. Least Absolute Shrinkage and Selection Operator (LASSO) and multifactorial cox regression were used to screen for signature genes associated with OC and used to establish prognostic models.</p><p><strong>Result: </strong>OC patients were categorized into 5 GTs clusters using consensus unsupervised cluster analysis. Clusters D and E showed significant differences between survival, signaling pathways and immune infiltration. Then, a risk model was developed based on the 12 signature genes, which provides a more accurate evaluation of the prognosis of OC patients. We categorized patients into high-risk and low-risk groups based on the risk score and found that the survival of patients in the high-risk group was significantly lower than that in the low-risk group. Moreover, the risk score was significantly correlated with tumor microenvironment, immune infiltration, and chemotherapy sensitivity.</p><p><strong>Conclusion: </strong>Overall, we performed a comprehensive analysis of GTs in OC patients and developed a risk model for OC. Our findings will provide a new insight to OC prognosis and treatment.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":"13 1","pages":"12-25"},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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American journal of clinical and experimental immunology
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