首页 > 最新文献

American journal of clinical and experimental immunology最新文献

英文 中文
Prognostic significance of LRPPRC and its association with immune infiltration in liver hepatocellular carcinoma. 肝肝细胞癌中 LRPPRC 的预后意义及其与免疫浸润的关系。
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/XTLJ1335
Yanqiu Zhang, Bin Feng, Yuting Liang, Qingqin Tang, Sheng Zhang, Zheng Zhang, Li Xu, Jingping Yin

Background: Leucine rich pentatricopeptide repeat containing (LRPPRC) protein is a multifunctional protein involved in cell cycle progression and tumor development. However, its prognostic significance and association with immune infiltration in Liver hepatocellular carcinoma (LIHC) remain unclear.

Methods: We utilized transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases of LIHC patients to investigate the potential pro-cancer role of LRPPRC, including differential expression of LRPPRC in LIHC, prognostic value, clinicopathological features, immune cell infiltration relevance and function enrichment analysis.

Results: Our findings suggest that LRPPRC is upregulated in LIHC and exhibits correlations with survival, clinical stage, and tumor grade in LIHC patients. Additionally, immune infiltration analysis revealed significant negative correlations between LRPPRC expression and multiple tumor-infiltrating immune cells, including CTLs, DCs, pDCs, B cells, Th17 cells, neutrophils, T cells, Mast cells, Th1 cells, Tregs, and NK cells, whereas a significant positive correlation was observed with infiltration of Th2 cells, T helper cells and Tcms. Furthermore, functional enrichment analysis indicated that LRPPRC may be involved in G2m checkpoint, mitotic spindle, E2f targets, Wnt Beta catenin signaling, spermatogenesis and other processes.

背景:富亮氨酸五肽重复蛋白(LRPPRC)是一种参与细胞周期进展和肿瘤发生的多功能蛋白。然而,它在肝肝细胞癌(LIHC)中的预后意义及其与免疫浸润的关系仍不清楚:我们利用癌症基因组图谱(TCGA)和基因型组织表达(GTEx)数据库中LIHC患者的转录组和临床数据研究了LRPPRC的潜在促癌作用,包括LRPPRC在LIHC中的差异表达、预后价值、临床病理特征、免疫细胞浸润相关性和功能富集分析:我们的研究结果表明,LRPPRC在LIHC中上调,并与LIHC患者的生存期、临床分期和肿瘤分级相关。此外,免疫浸润分析表明,LRPPRC 的表达与多种肿瘤浸润免疫细胞(包括 CTLs、DCs、pDCs、B 细胞、Th17 细胞、中性粒细胞、T 细胞、肥大细胞、Th1 细胞、Tregs 和 NK 细胞)呈显著负相关,而与 Th2 细胞、T 辅助细胞和 Tcms 的浸润呈显著正相关。此外,功能富集分析表明,LRPPRC 可能参与了 G2m 检查点、有丝分裂纺锤体、E2f 靶点、Wnt Beta 连环素信号转导、精子发生等过程。
{"title":"Prognostic significance of LRPPRC and its association with immune infiltration in liver hepatocellular carcinoma.","authors":"Yanqiu Zhang, Bin Feng, Yuting Liang, Qingqin Tang, Sheng Zhang, Zheng Zhang, Li Xu, Jingping Yin","doi":"10.62347/XTLJ1335","DOIUrl":"10.62347/XTLJ1335","url":null,"abstract":"<p><strong>Background: </strong>Leucine rich pentatricopeptide repeat containing (LRPPRC) protein is a multifunctional protein involved in cell cycle progression and tumor development. However, its prognostic significance and association with immune infiltration in Liver hepatocellular carcinoma (LIHC) remain unclear.</p><p><strong>Methods: </strong>We utilized transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases of LIHC patients to investigate the potential pro-cancer role of LRPPRC, including differential expression of LRPPRC in LIHC, prognostic value, clinicopathological features, immune cell infiltration relevance and function enrichment analysis.</p><p><strong>Results: </strong>Our findings suggest that LRPPRC is upregulated in LIHC and exhibits correlations with survival, clinical stage, and tumor grade in LIHC patients. Additionally, immune infiltration analysis revealed significant negative correlations between LRPPRC expression and multiple tumor-infiltrating immune cells, including CTLs, DCs, pDCs, B cells, Th17 cells, neutrophils, T cells, Mast cells, Th1 cells, Tregs, and NK cells, whereas a significant positive correlation was observed with infiltration of Th2 cells, T helper cells and Tcms. Furthermore, functional enrichment analysis indicated that LRPPRC may be involved in G2m checkpoint, mitotic spindle, E2f targets, Wnt Beta catenin signaling, spermatogenesis and other processes.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of macrophage-related genes in bladder cancer patients using single-cell sequencing and construction of a prognostic model. 利用单细胞测序鉴定膀胱癌患者的巨噬细胞相关基因并构建预后模型
IF 1.4 Q4 IMMUNOLOGY Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.62347/VLDZ7581
Weizhuo Wang, Junheng Shen, Dalong Song, Kai Fu, Xu Fu

Single-cell sequencing is an emerging technology that can effectively identify cell types in tumors. In the tumor microenvironment of bladder cancer, macrophages play a crucial role in invasion and immune escape. This study aimed to assess the expression of macrophage-related genes (MRGs) in the tumor microenvironment of bladder cancer patients and construct a prognostic model based on MRGs using bioinformatics methods.

Methods: Single-cell sequencing data from bladder cancer patients was downloaded from the GEO. After quality control and cell type identification, macrophages in the samples were extracted for re-clustering. Feature genes were then identified, and MRGs were assessed. Genetic data from TCGA database bladder cancer patients was also downloaded and organized. The intersection of MRGs and the TCGA gene set was determined. Clinical information was connected with this intersection, and the data was divided into training and validation sets. The training set was used for model construction and the validation set for model verification. A prognostic model based on MRGs was built using the LASSO algorithm and Cox regression. Patients were divided into high-risk and low-risk groups based on their prognostic features, and survival information in the training and validation sets was observed. The predictive ability of the model was assessed using a ROC curve, followed by a calibration plot to predict 1-, 3-, and 5-year survival rates.

Results: Four cell types were identified, and after extracting macrophages, three cell subgroups were clustered, resulting in 1,078 feature genes. The top 100 feature genes from each macrophage subgroup were extracted and intersected with TCGA expressed genes to construct the model. A risk prediction model composed of CD74, METRN, PTPRR, and CDC42EP5 was obtained. The survival and ROC curves showed that this model had good predictive ability. A calibration curve also demonstrated good prognostic ability for patients.

Conclusion: This study, based on single-cell data, TCGA data, and clinical information, constructed an MRG-based prognostic model for bladder cancer using multi-omics methods. This model has good accuracy and reliability in predicting the survival and prognosis of patients with bladder cancer, providing a reference for understanding the interaction between MRGs and bladder cancer.

单细胞测序是一种新兴技术,能有效识别肿瘤中的细胞类型。在膀胱癌的肿瘤微环境中,巨噬细胞对肿瘤的侵袭和免疫逃逸起着至关重要的作用。本研究旨在评估膀胱癌患者肿瘤微环境中巨噬细胞相关基因(MRGs)的表达情况,并利用生物信息学方法构建基于MRGs的预后模型:方法:从 GEO 下载膀胱癌患者的单细胞测序数据。方法:从 GEO 下载膀胱癌患者的单细胞测序数据,经过质量控制和细胞类型鉴定后,提取样本中的巨噬细胞进行重新聚类。然后确定特征基因,评估MRGs。此外,还从 TCGA 数据库下载并整理了膀胱癌患者的基因数据。确定MRGs与TCGA基因集的交集。临床信息与该交集相连,数据被分为训练集和验证集。训练集用于构建模型,验证集用于验证模型。利用 LASSO 算法和 Cox 回归建立了基于 MRGs 的预后模型。根据预后特征将患者分为高风险组和低风险组,并观察训练集和验证集的生存信息。使用 ROC 曲线评估了模型的预测能力,随后使用校准图预测了 1 年、3 年和 5 年的生存率:结果:确定了四种细胞类型,提取巨噬细胞后,对三个细胞亚群进行了聚类,得出了 1,078 个特征基因。从每个巨噬细胞亚群中提取前100个特征基因,并与TCGA表达基因交叉构建模型。得到了一个由CD74、METRN、PTPRR和CDC42EP5组成的风险预测模型。生存曲线和 ROC 曲线显示,该模型具有良好的预测能力。校准曲线也证明了该模型对患者具有良好的预后能力:本研究基于单细胞数据、TCGA 数据和临床信息,利用多组学方法构建了基于 MRG 的膀胱癌预后模型。该模型在预测膀胱癌患者的生存期和预后方面具有良好的准确性和可靠性,为了解MRGs与膀胱癌之间的相互作用提供了参考。
{"title":"Identification of macrophage-related genes in bladder cancer patients using single-cell sequencing and construction of a prognostic model.","authors":"Weizhuo Wang, Junheng Shen, Dalong Song, Kai Fu, Xu Fu","doi":"10.62347/VLDZ7581","DOIUrl":"10.62347/VLDZ7581","url":null,"abstract":"<p><p>Single-cell sequencing is an emerging technology that can effectively identify cell types in tumors. In the tumor microenvironment of bladder cancer, macrophages play a crucial role in invasion and immune escape. This study aimed to assess the expression of macrophage-related genes (MRGs) in the tumor microenvironment of bladder cancer patients and construct a prognostic model based on MRGs using bioinformatics methods.</p><p><strong>Methods: </strong>Single-cell sequencing data from bladder cancer patients was downloaded from the GEO. After quality control and cell type identification, macrophages in the samples were extracted for re-clustering. Feature genes were then identified, and MRGs were assessed. Genetic data from TCGA database bladder cancer patients was also downloaded and organized. The intersection of MRGs and the TCGA gene set was determined. Clinical information was connected with this intersection, and the data was divided into training and validation sets. The training set was used for model construction and the validation set for model verification. A prognostic model based on MRGs was built using the LASSO algorithm and Cox regression. Patients were divided into high-risk and low-risk groups based on their prognostic features, and survival information in the training and validation sets was observed. The predictive ability of the model was assessed using a ROC curve, followed by a calibration plot to predict 1-, 3-, and 5-year survival rates.</p><p><strong>Results: </strong>Four cell types were identified, and after extracting macrophages, three cell subgroups were clustered, resulting in 1,078 feature genes. The top 100 feature genes from each macrophage subgroup were extracted and intersected with TCGA expressed genes to construct the model. A risk prediction model composed of CD74, METRN, PTPRR, and CDC42EP5 was obtained. The survival and ROC curves showed that this model had good predictive ability. A calibration curve also demonstrated good prognostic ability for patients.</p><p><strong>Conclusion: </strong>This study, based on single-cell data, TCGA data, and clinical information, constructed an MRG-based prognostic model for bladder cancer using multi-omics methods. This model has good accuracy and reliability in predicting the survival and prognosis of patients with bladder cancer, providing a reference for understanding the interaction between MRGs and bladder cancer.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11249859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insight into NSCLC through novel analysis of gene interactions and characteristics. 通过对基因相互作用和特征的新分析,深入了解 NSCLC。
Pub Date : 2024-04-25 eCollection Date: 2024-01-01 DOI: 10.62347/ANLV4963
Eric Pan, Yongsheng Bai

Around 80 to 85% of all lung cancers are non-small cell lung cancer (NSCLC). Previous research has aimed at exploring the genetic basis of NSCLC through individual approaches, but studies have yet to investigate the results of combining them. Here we show that analyzing NSCLC genetics through three approaches simultaneously creates unique insights into our understanding of the disease. Through a combination of previous research and bioinformatics tools, we determined 35 NSCLC candidate genes. We analyzed these genes in 3 different approaches. First, we found the gene fusions between these candidate genes. Second, we found the common superfamilies between genes. Finally, we identified mutational signatures that are possibly associated with NSCLC. Each approach has its individual, unique results. Fusion relationships identify specific gene fusion targets, common superfamilies identify possible avenues to determine novel target genes, and identifying NSCLC associated mutational signatures has diagnostic and prognostic benefits. Combining the approaches, we found that gene CD74 has significant fusion relationships, but it has no association with the other two approaches, suggesting that CD74 is associated with NSCLC mainly because of its fusion relationships. Targeting the gene fusions of CD74 may be an alternative NSCLC treatment. This genetic analysis has indeed created unique insight into NSCLC genes. Both the results from each of the approaches separately and combined allow pursuit of more effective treatment strategies for this cancer. The methodology presented can also apply to other cancers, creating insights that current analytical methods could not find.

大约 80% 至 85% 的肺癌属于非小细胞肺癌(NSCLC)。以往的研究旨在通过单独的方法探索非小细胞肺癌的遗传基础,但尚未研究将这些方法结合起来的结果。在这里,我们展示了通过三种方法同时分析 NSCLC 遗传学能为我们了解这种疾病提供独特的见解。通过结合以往的研究和生物信息学工具,我们确定了 35 个 NSCLC 候选基因。我们用三种不同的方法对这些基因进行了分析。首先,我们发现了这些候选基因之间的基因融合。其次,我们发现了基因之间的共同超家族。最后,我们确定了可能与 NSCLC 相关的突变特征。每种方法都有其各自独特的结果。融合关系确定了特定的基因融合靶点,共同超家族确定了确定新靶基因的可能途径,而确定与 NSCLC 相关的突变特征则具有诊断和预后方面的益处。综合上述方法,我们发现 CD74 基因有显著的融合关系,但与其他两种方法没有关联,这表明 CD74 与 NSCLC 相关主要是因为其融合关系。针对 CD74 的基因融合可能是治疗 NSCLC 的另一种方法。这项基因分析确实对 NSCLC 基因有了独特的见解。无论是将每种方法的结果分开还是结合起来,都可以为这种癌症寻求更有效的治疗策略。所介绍的方法也可应用于其他癌症,从而提出目前的分析方法无法发现的见解。
{"title":"Insight into NSCLC through novel analysis of gene interactions and characteristics.","authors":"Eric Pan, Yongsheng Bai","doi":"10.62347/ANLV4963","DOIUrl":"10.62347/ANLV4963","url":null,"abstract":"<p><p>Around 80 to 85% of all lung cancers are non-small cell lung cancer (NSCLC). Previous research has aimed at exploring the genetic basis of NSCLC through individual approaches, but studies have yet to investigate the results of combining them. Here we show that analyzing NSCLC genetics through three approaches simultaneously creates unique insights into our understanding of the disease. Through a combination of previous research and bioinformatics tools, we determined 35 NSCLC candidate genes. We analyzed these genes in 3 different approaches. First, we found the gene fusions between these candidate genes. Second, we found the common superfamilies between genes. Finally, we identified mutational signatures that are possibly associated with NSCLC. Each approach has its individual, unique results. Fusion relationships identify specific gene fusion targets, common superfamilies identify possible avenues to determine novel target genes, and identifying NSCLC associated mutational signatures has diagnostic and prognostic benefits. Combining the approaches, we found that gene CD74 has significant fusion relationships, but it has no association with the other two approaches, suggesting that CD74 is associated with NSCLC mainly because of its fusion relationships. Targeting the gene fusions of CD74 may be an alternative NSCLC treatment. This genetic analysis has indeed created unique insight into NSCLC genes. Both the results from each of the approaches separately and combined allow pursuit of more effective treatment strategies for this cancer. The methodology presented can also apply to other cancers, creating insights that current analytical methods could not find.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IFN-γ, IL-17, IL-22+ CD4+ subset in patients with hepatitis C virus and correlation with clinical factor. 丙型肝炎病毒感染者的 IFN-γ、IL-17、IL-22+ CD4+ 亚群及其与临床因素的相关性。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Soolmaz Khansalar, Zahra Faghih, Shaghik Barani, Mehdi Kalani, Mohammad Reza Ataollahi, Zeinab Mohammadi, Sepideh Namdari, Kurosh Kalantar

Background: CD4+ T cell responses in HCV infection have a crucial role in the immunopathology of hepatitis C virus (HCV) infection. Our aim was to investigate the frequency of Th1, Th17, and Th22 cells in HCV-infected patients and elucidate their role in the progression of the disease.

Methods: Twenty-six HCV-infected patients and 26 healthy individuals were recruited. Peripheral blood mononuclear cells (PBMCs) were stained to separate CD4, IFN-γ, IL-17, and IL-22 producing cells using flow cytometry.

Results: Results showed that the mean expression of IL-22 in CD4+ T cells was significantly lower in HCV-infected patients compared to healthy controls. About correlation with clinical factor and T subsets, a negative correlation between the frequency of CD4+ IFN-γ+ cells and Thyroxine level (T4) was observed in the patients. The data showed a positive link between thyroid-stimulating hormone (TSH), cholesterol levels, and the frequency of Th17 cells. In addition, a positive correlation was seen between serum creatinine level with both Th1 and Th17. Ultimately, it was found that there was a positive link between viral burden and IL-17+ IL-22+ cells and a negative correlation between viral load and pure Th22.

Conclusions: Our findings indicate that Th22 cells may play a part in the immunopathology of HCV and show the associations between Thelper subsets and the clinical signs of the disease.

背景:HCV感染中的CD4+T细胞反应在丙型肝炎病毒(HCV)感染的免疫病理学中起着至关重要的作用。我们的目的是调查 HCV 感染者体内 Th1、Th17 和 Th22 细胞的频率,并阐明它们在疾病进展中的作用:方法:我们招募了 26 名 HCV 感染者和 26 名健康人。采用流式细胞术对外周血单核细胞(PBMCs)进行染色,以分离产生 CD4、IFN-γ、IL-17 和 IL-22 的细胞:结果显示:与健康对照组相比,HCV 感染者 CD4+ T 细胞中 IL-22 的平均表达量明显较低。关于与临床因素和 T 亚群的相关性,在患者中观察到 CD4+ IFN-γ+ 细胞的频率与甲状腺素水平(T4)呈负相关。数据显示,促甲状腺激素(TSH)、胆固醇水平与 Th17 细胞的频率呈正相关。此外,血清肌酐水平与 Th1 和 Th17 细胞之间也存在正相关。最后,研究发现病毒负荷与 IL-17+ IL-22+ 细胞之间存在正相关,而病毒负荷与纯 Th22 细胞之间存在负相关:我们的研究结果表明,Th22细胞可能在HCV的免疫病理中起一定作用,并显示了Thlper亚群与疾病临床症状之间的关联。
{"title":"IFN-γ, IL-17, IL-22<sup>+</sup> CD4<sup>+</sup> subset in patients with hepatitis C virus and correlation with clinical factor.","authors":"Soolmaz Khansalar, Zahra Faghih, Shaghik Barani, Mehdi Kalani, Mohammad Reza Ataollahi, Zeinab Mohammadi, Sepideh Namdari, Kurosh Kalantar","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>CD4<sup>+</sup> T cell responses in HCV infection have a crucial role in the immunopathology of hepatitis C virus (HCV) infection. Our aim was to investigate the frequency of Th1, Th17, and Th22 cells in HCV-infected patients and elucidate their role in the progression of the disease.</p><p><strong>Methods: </strong>Twenty-six HCV-infected patients and 26 healthy individuals were recruited. Peripheral blood mononuclear cells (PBMCs) were stained to separate CD4, IFN-γ, IL-17, and IL-22 producing cells using flow cytometry.</p><p><strong>Results: </strong>Results showed that the mean expression of IL-22 in CD4<sup>+</sup> T cells was significantly lower in HCV-infected patients compared to healthy controls. About correlation with clinical factor and T subsets, a negative correlation between the frequency of CD4<sup>+</sup> IFN-γ<sup>+</sup> cells and Thyroxine level (T4) was observed in the patients. The data showed a positive link between thyroid-stimulating hormone (TSH), cholesterol levels, and the frequency of Th17 cells. In addition, a positive correlation was seen between serum creatinine level with both Th1 and Th17. Ultimately, it was found that there was a positive link between viral burden and IL-17<sup>+</sup> IL-22<sup>+</sup> cells and a negative correlation between viral load and pure Th22.</p><p><strong>Conclusions: </strong>Our findings indicate that Th22 cells may play a part in the immunopathology of HCV and show the associations between Thelper subsets and the clinical signs of the disease.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reevaluating elevated HDL cholesterol levels in healthy older persons as a risk factor for various disease states. 重新评估健康老年人高密度脂蛋白胆固醇水平升高作为各种疾病风险因素的情况。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Ren-Xin Ji, Zhou-Ying Duan

This article reviews the role of high-density lipoprotein cholesterol (HDL-C) in the elderly population, questioning the established view that advocates the ubiquitous health benefits of HDL cholesterol. High levels of HDL-C have been found to be associated with an increased risk of debilitating fractures, dementia, and cardiovascular disease, predominantly affecting older men, through the use of large population-based studies such as the ASPREE trial and the UK Biobank. Possible mechanisms are closely linked to cholesterol crystallization and altered HDL particle function. These findings call for a refinement of the understanding of high-density lipoprotein cholesterol (HDL-C), which implies adjustments to clinical guidelines and risk assessment strategies in older populations.

本文回顾了高密度脂蛋白胆固醇(HDL-C)在老年人群中的作用,对鼓吹高密度脂蛋白胆固醇无处不在的健康益处的既定观点提出了质疑。通过使用大型人群研究(如 ASPREE 试验和英国生物库)发现,高密度脂蛋白胆固醇水平与骨折、痴呆症和心血管疾病的风险增加有关,这些疾病主要影响老年男性。可能的机制与胆固醇结晶和高密度脂蛋白颗粒功能的改变密切相关。这些发现要求完善对高密度脂蛋白胆固醇(HDL-C)的认识,这意味着要调整老年人群的临床指南和风险评估策略。
{"title":"Reevaluating elevated HDL cholesterol levels in healthy older persons as a risk factor for various disease states.","authors":"Ren-Xin Ji, Zhou-Ying Duan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This article reviews the role of high-density lipoprotein cholesterol (HDL-C) in the elderly population, questioning the established view that advocates the ubiquitous health benefits of HDL cholesterol. High levels of HDL-C have been found to be associated with an increased risk of debilitating fractures, dementia, and cardiovascular disease, predominantly affecting older men, through the use of large population-based studies such as the ASPREE trial and the UK Biobank. Possible mechanisms are closely linked to cholesterol crystallization and altered HDL particle function. These findings call for a refinement of the understanding of high-density lipoprotein cholesterol (HDL-C), which implies adjustments to clinical guidelines and risk assessment strategies in older populations.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the role of NAA40 in immune infiltrates and prognostic prediction in hepatocellular carcinoma. 探索 NAA40 在肝细胞癌免疫浸润和预后预测中的作用。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Tong Zhou, Jun Cao, Qingqin Tang, Jieyu Jin, Yuting Liang, Bin Feng

NAA40 belongs to the N-terminal acetyltransferase (NATs) family, responsible for protein N-terminal modification, and it exerts crucial roles across various cancers. However, its impact on patient prognosis and immune infiltration in hepatocellular carcinoma (HCC) remains elusive. To address this, our study delved into the comprehensive analysis of NAA40 in the context of cancer. Our pan-cancer analysis unveiled elevated NAA40 expression in multiple tumor types, including BLCA, BRCA, CHOL, COAD, ESCA, HNSC, LIHC, LUAD, LUSC, STAD, and THCA. Additionally, through a comprehensive examination across various cancer types within TCGA, we discovered that high NAA40 gene expression correlated with poor prognosis in HCC, pointing toward its role in promoting oncogenesis. Further investigation illuminated the association of increased NAA40 expression with T stage, pathologic stage, tumor status, and histologic grade. Interestingly, we noted a significant inverse correlation between NAA40 expression and the infiltration levels of immune cells, such as DC cells, neutrophils, NK cells, and T cells, in liver cancer. This observation underpins the hypothesis that NAA40 influences HCC development by modulating immune cell infiltration. Functional enrichment analysis provided valuable insights into the pathways influenced by NAA40. Enriched pathways encompassed oxidative phosphorylation, xenobiotic metabolism, bile acid metabolism, fatty acid metabolism, G2M checkpoint, and E2F targets. These findings collectively position NAA40 as a potential biomarker for prognostic prediction and monitoring the effects of immunotherapy in HCC.

NAA40属于N端乙酰转移酶(NATs)家族,负责蛋白质的N端修饰,在各种癌症中发挥着重要作用。然而,它对肝细胞癌(HCC)患者预后和免疫浸润的影响仍然难以捉摸。为了解决这个问题,我们的研究深入研究了癌症背景下 NAA40 的全面分析。我们的泛癌症分析揭示了NAA40在多种肿瘤类型中的高表达,包括BLCA、BRCA、CHOL、COAD、ESCA、HNSC、LIHC、LUAD、LUSC、STAD和THCA。此外,通过对TCGA中各种癌症类型的全面检查,我们发现NAA40基因的高表达与HCC的不良预后相关,这表明NAA40在促进肿瘤发生中的作用。进一步的研究表明,NAA40表达的增加与T分期、病理分期、肿瘤状态和组织学分级有关。有趣的是,我们注意到NAA40的表达与肝癌中免疫细胞(如DC细胞、中性粒细胞、NK细胞和T细胞)的浸润水平呈显著的反相关。这一观察结果支持了NAA40通过调节免疫细胞浸润影响HCC发展的假设。功能富集分析为了解受NAA40影响的通路提供了宝贵的信息。富集途径包括氧化磷酸化、异生物代谢、胆汁酸代谢、脂肪酸代谢、G2M检查点和E2F靶点。这些发现共同将NAA40定位为一种潜在的生物标记物,用于预测HCC的预后和监测免疫疗法的效果。
{"title":"Exploring the role of NAA40 in immune infiltrates and prognostic prediction in hepatocellular carcinoma.","authors":"Tong Zhou, Jun Cao, Qingqin Tang, Jieyu Jin, Yuting Liang, Bin Feng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>NAA40 belongs to the N-terminal acetyltransferase (NATs) family, responsible for protein N-terminal modification, and it exerts crucial roles across various cancers. However, its impact on patient prognosis and immune infiltration in hepatocellular carcinoma (HCC) remains elusive. To address this, our study delved into the comprehensive analysis of NAA40 in the context of cancer. Our pan-cancer analysis unveiled elevated NAA40 expression in multiple tumor types, including BLCA, BRCA, CHOL, COAD, ESCA, HNSC, LIHC, LUAD, LUSC, STAD, and THCA. Additionally, through a comprehensive examination across various cancer types within TCGA, we discovered that high NAA40 gene expression correlated with poor prognosis in HCC, pointing toward its role in promoting oncogenesis. Further investigation illuminated the association of increased NAA40 expression with T stage, pathologic stage, tumor status, and histologic grade. Interestingly, we noted a significant inverse correlation between NAA40 expression and the infiltration levels of immune cells, such as DC cells, neutrophils, NK cells, and T cells, in liver cancer. This observation underpins the hypothesis that NAA40 influences HCC development by modulating immune cell infiltration. Functional enrichment analysis provided valuable insights into the pathways influenced by NAA40. Enriched pathways encompassed oxidative phosphorylation, xenobiotic metabolism, bile acid metabolism, fatty acid metabolism, G2M checkpoint, and E2F targets. These findings collectively position NAA40 as a potential biomarker for prognostic prediction and monitoring the effects of immunotherapy in HCC.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of glycosyltransferase-related genes signature and integrative analyses in patients with ovarian cancer. 鉴定卵巢癌患者的糖基转移酶相关基因特征并进行综合分析。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Yanqiu Zhang, Tong Zhou, Qingqin Tang, Bin Feng, Yuting Liang

Background: Glycosyltransferases (GT) play a crucial role in glycosylation reactions, and aberrant expression of glycosyltransferase-related genes (GTs) leads to abnormal glycosylation, which is associated with tumor progression. However, the prognostic value of aberrant expression of GTs in ovarian cancer (OC) and the correlation between GTs and tumor microenvironment (TME) remain unknown.

Methods: TCGA and GSE53963 databases were used to obtain data on OC patient samples. The association of GTs with OC was analyzed. Molecular subtypes were identified by consensus unsupervised clustering, followed by immune infiltration and functional enrichment analyses. Survival analysis was performed using Kaplan-Meier curves and log-rank tests. Least Absolute Shrinkage and Selection Operator (LASSO) and multifactorial cox regression were used to screen for signature genes associated with OC and used to establish prognostic models.

Result: OC patients were categorized into 5 GTs clusters using consensus unsupervised cluster analysis. Clusters D and E showed significant differences between survival, signaling pathways and immune infiltration. Then, a risk model was developed based on the 12 signature genes, which provides a more accurate evaluation of the prognosis of OC patients. We categorized patients into high-risk and low-risk groups based on the risk score and found that the survival of patients in the high-risk group was significantly lower than that in the low-risk group. Moreover, the risk score was significantly correlated with tumor microenvironment, immune infiltration, and chemotherapy sensitivity.

Conclusion: Overall, we performed a comprehensive analysis of GTs in OC patients and developed a risk model for OC. Our findings will provide a new insight to OC prognosis and treatment.

背景:糖基转移酶(GT)在糖基化反应中起着至关重要的作用,糖基转移酶相关基因(GTs)的异常表达会导致糖基化异常,而糖基化异常与肿瘤进展有关。然而,GTs异常表达在卵巢癌(OC)中的预后价值以及GTs与肿瘤微环境(TME)之间的相关性仍然未知:方法:利用TCGA和GSE53963数据库获取OC患者样本数据。方法:利用 TCGA 和 GSE53963 数据库获取 OC 患者样本数据,分析 GTs 与 OC 的关联。通过共识无监督聚类确定分子亚型,然后进行免疫浸润和功能富集分析。利用卡普兰-梅耶曲线和对数秩检验进行了生存分析。利用最小绝对收缩和选择操作器(LASSO)和多因素cox回归筛选与OC相关的特征基因,并用于建立预后模型:结果:采用共识无监督聚类分析将OC患者分为5个GTs群。聚类D和E在生存、信号通路和免疫浸润方面存在显著差异。然后,根据这12个特征基因建立了一个风险模型,该模型能更准确地评估OC患者的预后。我们根据风险评分将患者分为高风险组和低风险组,发现高风险组患者的生存率明显低于低风险组。此外,风险评分与肿瘤微环境、免疫浸润和化疗敏感性有明显相关性:总之,我们对OC患者的GT进行了全面分析,并建立了OC的风险模型。总之,我们对 OC 患者的 GTs 进行了全面分析,并建立了 OC 的风险模型,我们的研究结果将为 OC 的预后和治疗提供新的见解。
{"title":"Identification of glycosyltransferase-related genes signature and integrative analyses in patients with ovarian cancer.","authors":"Yanqiu Zhang, Tong Zhou, Qingqin Tang, Bin Feng, Yuting Liang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Glycosyltransferases (GT) play a crucial role in glycosylation reactions, and aberrant expression of glycosyltransferase-related genes (GTs) leads to abnormal glycosylation, which is associated with tumor progression. However, the prognostic value of aberrant expression of GTs in ovarian cancer (OC) and the correlation between GTs and tumor microenvironment (TME) remain unknown.</p><p><strong>Methods: </strong>TCGA and GSE53963 databases were used to obtain data on OC patient samples. The association of GTs with OC was analyzed. Molecular subtypes were identified by consensus unsupervised clustering, followed by immune infiltration and functional enrichment analyses. Survival analysis was performed using Kaplan-Meier curves and log-rank tests. Least Absolute Shrinkage and Selection Operator (LASSO) and multifactorial cox regression were used to screen for signature genes associated with OC and used to establish prognostic models.</p><p><strong>Result: </strong>OC patients were categorized into 5 GTs clusters using consensus unsupervised cluster analysis. Clusters D and E showed significant differences between survival, signaling pathways and immune infiltration. Then, a risk model was developed based on the 12 signature genes, which provides a more accurate evaluation of the prognosis of OC patients. We categorized patients into high-risk and low-risk groups based on the risk score and found that the survival of patients in the high-risk group was significantly lower than that in the low-risk group. Moreover, the risk score was significantly correlated with tumor microenvironment, immune infiltration, and chemotherapy sensitivity.</p><p><strong>Conclusion: </strong>Overall, we performed a comprehensive analysis of GTs in OC patients and developed a risk model for OC. Our findings will provide a new insight to OC prognosis and treatment.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
cfDNA from maternal plasma for noninvasive screening of fetal exomes. 利用母体血浆中的 cfDNA 对胎儿外显子进行无创筛查。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Longwei Qiao

In recent years, a shift in prenatal screening methods has been observed, moving away from traditional approaches such as ultrasound and maternal serologic markers towards the utilization of noninvasive prenatal testing (NIPT) based on cfDNA extracted from peripheral blood. This cutting-edge technology has established itself as the primary screening method, attributed to its superior detection rate and reduced false-positive rate. Although NIPT predominantly focuses on screening for chromosomal abnormalities, it currently does not encompass the identification of single-gene disorders. Considering that single-gene disorders contribute significantly to birth defects, accounting for 7.5% to 12% of cases, it becomes imperative to integrate screening for single-gene disorders into the birth defect prevention and control system. This study aims to provide a succinct overview of the recent advancements in NIPT specifically tailored for monogenic disorders.

近年来,产前筛查方法发生了转变,从超声波和母体血清学标记等传统方法转向使用基于外周血中提取的 cfDNA 的无创产前检测(NIPT)。这项尖端技术因其卓越的检测率和较低的假阳性率,已成为主要的筛查方法。虽然 NIPT 主要侧重于筛查染色体异常,但目前还不包括单基因疾病的鉴定。考虑到单基因遗传病在出生缺陷中的比例高达 7.5% 至 12%,将单基因遗传病筛查纳入出生缺陷防控体系势在必行。本研究旨在简明扼要地概述专门针对单基因疾病的 NIPT 的最新进展。
{"title":"cfDNA from maternal plasma for noninvasive screening of fetal exomes.","authors":"Longwei Qiao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In recent years, a shift in prenatal screening methods has been observed, moving away from traditional approaches such as ultrasound and maternal serologic markers towards the utilization of noninvasive prenatal testing (NIPT) based on cfDNA extracted from peripheral blood. This cutting-edge technology has established itself as the primary screening method, attributed to its superior detection rate and reduced false-positive rate. Although NIPT predominantly focuses on screening for chromosomal abnormalities, it currently does not encompass the identification of single-gene disorders. Considering that single-gene disorders contribute significantly to birth defects, accounting for 7.5% to 12% of cases, it becomes imperative to integrate screening for single-gene disorders into the birth defect prevention and control system. This study aims to provide a succinct overview of the recent advancements in NIPT specifically tailored for monogenic disorders.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of pulmonary rehabilitation on systemic inflammation in chronic obstructive pulmonary disease: a meta-analysis. 肺康复对慢性阻塞性肺病全身炎症的影响:一项荟萃分析。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Xiaotian Alex Yue, Yilan Sheng, Jianhua Li

Chronic obstructive pulmonary disease (COPD) is marked by both lung-related and systemic symptoms, notably chronic inflammation. Despite pulmonary rehabilitation (PR) being a critical treatment for COPD, its influence on systemic inflammation remains unclear. This meta-analysis was conducted to assess PR's effect on circulating inflammatory markers in COPD patients. We systematically reviewed databases like PubMed, EMBASE, and Web of Science to select randomized controlled trials and observational studies that investigated the impact of PR on systemic inflammation. We calculated the mean differences (MD) in inflammatory markers before and after PR using a random-effects model and assessed the risk of bias with established tools. Our study included six investigations (four RCTs, two observational) with 147 COPD patients. Our findings show notable increases in IL-6 (MD 0.44, 95% CI 0.17-0.70, P = 0.001), CRP (MD 0.56, 95% CI 0.31-0.81, P<0.00001), and TNF-alpha (MD 0.41, 95% CI 0.12-0.70, P = 0.005) following PR. However, sensitivity analysis pinpointed the study by El-Kader et al. as a key influence on these results. Excluding this study led to nonsignificant changes. Thus, our meta-analysis uncovers an unanticipated rise in inflammatory markers post-PR in COPD patients, questioning the assumed anti-inflammatory benefits of PR.

慢性阻塞性肺疾病(COPD)既有肺部相关症状,也有全身症状,尤其是慢性炎症。尽管肺康复(PR)是慢性阻塞性肺病的一种重要治疗方法,但其对全身炎症的影响仍不明确。本荟萃分析旨在评估肺康复对慢性阻塞性肺病患者循环炎症标志物的影响。我们系统地查阅了 PubMed、EMBASE 和 Web of Science 等数据库,选择了调查 PR 对全身炎症影响的随机对照试验和观察性研究。我们使用随机效应模型计算了 PR 前后炎症指标的平均差异 (MD),并使用既定工具评估了偏倚风险。我们的研究包括六项调查(四项 RCT,两项观察性研究),涉及 147 名慢性阻塞性肺病患者。
{"title":"Effects of pulmonary rehabilitation on systemic inflammation in chronic obstructive pulmonary disease: a meta-analysis.","authors":"Xiaotian Alex Yue, Yilan Sheng, Jianhua Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chronic obstructive pulmonary disease (COPD) is marked by both lung-related and systemic symptoms, notably chronic inflammation. Despite pulmonary rehabilitation (PR) being a critical treatment for COPD, its influence on systemic inflammation remains unclear. This meta-analysis was conducted to assess PR's effect on circulating inflammatory markers in COPD patients. We systematically reviewed databases like PubMed, EMBASE, and Web of Science to select randomized controlled trials and observational studies that investigated the impact of PR on systemic inflammation. We calculated the mean differences (MD) in inflammatory markers before and after PR using a random-effects model and assessed the risk of bias with established tools. Our study included six investigations (four RCTs, two observational) with 147 COPD patients. Our findings show notable increases in IL-6 (MD 0.44, 95% CI 0.17-0.70, P = 0.001), CRP (MD 0.56, 95% CI 0.31-0.81, P<0.00001), and TNF-alpha (MD 0.41, 95% CI 0.12-0.70, P = 0.005) following PR. However, sensitivity analysis pinpointed the study by El-Kader et al. as a key influence on these results. Excluding this study led to nonsignificant changes. Thus, our meta-analysis uncovers an unanticipated rise in inflammatory markers post-PR in COPD patients, questioning the assumed anti-inflammatory benefits of PR.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retrospective analysis of laboratory results in 18 cases of severe asthma treated with omalizumab. 对使用奥马珠单抗治疗的 18 例重症哮喘患者的化验结果进行回顾性分析。
Pub Date : 2024-02-25 eCollection Date: 2024-01-01
Wei-Ze Li, Yi-Qin Ge, Xuan-Yue Qu, Yi Liu, Cheng-Jian Lv, Jia Li, Juan Wang, Li Li, Xia Peng

Objective: The aim of this study was to explore the laboratory results in severe as asthma patients with omalizumab therapy and provide evidence for estimating omalizumab efficacy.

Methods: Retrospective study of 18 patients with severe asthma received omalizumab therapy in Shanghai General Hospital from 2020 to 2022 was performed. The basic data of patients were collected. The absolute number and the percentage of basophil and eosinophil in peripheral blood, total IgE level in serum, and as pulmonary function were detected at the beginning of treatment and 4 months after treatment. Differences between two groups were analyzed using Paired T test.

Results: The most common allergens collected from patients with moderate to severe asthma were dust mite (positive ratio 55.56%), mixed mold (16.67%), cat and dog dander, and Aspergillus fumigatus (11.11%). There was no significant difference in eosinophil and basophil counts in peripheral blood between the two groups. However, serum total IgE levels increased from (437.55±279.35) KU/L to (1071.42±721.28) KU/L (P=0.004), and FEV1/FVC ratio increased from (65.53±14.15)% to (73.91±13.63)% (P=0.005) after 4 months of treatment.

Conclusions: The existing laboratory indicators for evaluation of omalizumab efficacy are still very limited, and new biomarkers need to be further developed. Elevated serum IgE levels at four weeks of treatment and FEV1/FVC may be potential indicators for omalizumab monitoring.

研究目的本研究旨在探讨接受奥马珠单抗治疗的重症哮喘患者的实验室结果,为估算奥马珠单抗的疗效提供证据:方法:对2020年至2022年在上海总医院接受奥马珠单抗治疗的18例重症哮喘患者进行回顾性研究。收集了患者的基本数据。在治疗开始时和治疗 4 个月后检测外周血中嗜碱性粒细胞和嗜酸性粒细胞的绝对数量和百分比、血清总 IgE 水平以及肺功能。两组间的差异采用配对 T 检验进行分析:从中重度哮喘患者身上收集到的最常见过敏原是尘螨(阳性率为 55.56%)、混合霉菌(16.67%)、猫和狗的皮屑以及曲霉菌(11.11%)。两组患者外周血中的嗜酸性粒细胞和嗜碱性粒细胞计数无明显差异。然而,治疗4个月后,血清总IgE水平从(437.55±279.35)KU/L升高至(1071.42±721.28)KU/L(P=0.004),FEV1/FVC比值从(65.53±14.15)%升高至(73.91±13.63)%(P=0.005):现有的评估奥马珠单抗疗效的实验室指标仍然非常有限,需要进一步开发新的生物标志物。治疗四周时血清 IgE 水平升高和 FEV1/FVC 可能是监测奥马珠单抗的潜在指标。
{"title":"Retrospective analysis of laboratory results in 18 cases of severe asthma treated with omalizumab.","authors":"Wei-Ze Li, Yi-Qin Ge, Xuan-Yue Qu, Yi Liu, Cheng-Jian Lv, Jia Li, Juan Wang, Li Li, Xia Peng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to explore the laboratory results in severe as asthma patients with omalizumab therapy and provide evidence for estimating omalizumab efficacy.</p><p><strong>Methods: </strong>Retrospective study of 18 patients with severe asthma received omalizumab therapy in Shanghai General Hospital from 2020 to 2022 was performed. The basic data of patients were collected. The absolute number and the percentage of basophil and eosinophil in peripheral blood, total IgE level in serum, and as pulmonary function were detected at the beginning of treatment and 4 months after treatment. Differences between two groups were analyzed using Paired T test.</p><p><strong>Results: </strong>The most common allergens collected from patients with moderate to severe asthma were dust mite (positive ratio 55.56%), mixed mold (16.67%), cat and dog dander, and Aspergillus fumigatus (11.11%). There was no significant difference in eosinophil and basophil counts in peripheral blood between the two groups. However, serum total IgE levels increased from (437.55±279.35) KU/L to (1071.42±721.28) KU/L (P=0.004), and FEV1/FVC ratio increased from (65.53±14.15)% to (73.91±13.63)% (P=0.005) after 4 months of treatment.</p><p><strong>Conclusions: </strong>The existing laboratory indicators for evaluation of omalizumab efficacy are still very limited, and new biomarkers need to be further developed. Elevated serum IgE levels at four weeks of treatment and FEV1/FVC may be potential indicators for omalizumab monitoring.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10944360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
American journal of clinical and experimental immunology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1