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High-fat/high-fructose diet and Opisthorchis viverrini infection promote metabolic dysfunction-associated steatotic liver disease via inflammation, fibrogenesis, and metabolic dysfunction 高脂肪/高果糖饮食和猪腹蛇感染通过炎症、纤维生成和代谢功能障碍促进代谢功能障碍相关的脂肪变性肝病。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107491
Lakhanawan Charoensuk , Phonpilas Thongpon , Chutima Sitthirach , Apisit Chaidee , Kitti Intuyod , Chawalit Pairojkul , Ei Htet Htet Khin , Chanakan Jantawong , Kanjana Thumanu , Porntip Pinlaor , Nuttanan Hongsrichan , Somchai Pinlaor
Metabolic dysfunction-associated steatotic liver disease (MASLD) and opisthorchiasis, caused by Opisthorchis viverrini (O. viverrini) infection, frequently co-exist in Northeast Thailand. However, the underlying pathophysiology remains unknown. We aimed to investigate the effect of a high-fat/high-fructose (HFF) diet combined with O. viverrini infection on MASLD. Four groups each of ten male golden hamsters were established: normal controls (NC), O. viverrini-infected (OV), HFF-fed, and HFF-fed plus O. viverrini infection (HFF+OV). After four months of treatment, histopathological study indicated substantial hepatic damage in groups given the HFF diet. In particular, the HFF+OV group demonstrated marked lipid-droplet accumulation, hepatocyte ballooning, inflammatory-cell clustering, and widespread fibrosis. Biochemical tests indicated that the HFF+OV group had the highest concentrations of alanine aminotransferase and triglycerides, but cholesterol and low-density lipoprotein levels had increased in both HFF groups. Increased expression of Tgf-β1 and α-SMA, indicative of greater fibrosis, was demonstrated by picrosirius-red staining in the HFF+OV group. There was a significant increase in levels of inflammatory markers (HMGB-1, p65, and F4/80) and expression of genes related to the synthesis of fatty acids and glucose. FTIR microspectroscopy revealed distinct changes in fatty acids and proteins, associated with the more pronounced histopathology and impaired liver function in the HFF+OV group. The findings indicate that the interplay of a HFF diet and O. viverrini infection aggravates the progression of MASLD by augmenting liver damage, inflammation, fibrogenesis, and metabolic dysfunction. This study highlights the significance of incorporating both nutritional and infection factors into the management of liver disorders, especially in areas where opisthorchiasis is common.
在泰国东北部,代谢功能障碍相关的脂肪变性肝病(MASLD)和由猪胸虫(O. viverrini)感染引起的蛇胸虫病经常共存。然而,其潜在的病理生理机制尚不清楚。我们的目的是研究高脂肪/高果糖(HFF)饮食与O. viverrini感染对MASLD的影响。每10只雄性金仓鼠设4组,分别为正常对照组(NC)、活弧菌感染组(OV)、HFF喂养组和HFF喂养加活弧菌感染组(HFF+OV)。治疗4个月后,组织病理学研究表明,给予HFF饮食的组肝损伤明显。特别是,HFF+OV组表现出明显的脂滴积聚、肝细胞球囊、炎症细胞聚集和广泛的纤维化。生化试验表明,HFF+OV组丙氨酸转氨酶和甘油三酯浓度最高,但两组的胆固醇和低密度脂蛋白水平均升高。HFF+OV组小天狼星红染色显示Tgf-β1和α-SMA表达增加,表明纤维化程度加重。炎症标志物(HMGB-1、p65和F4/80)水平以及脂肪酸和葡萄糖合成相关基因的表达显著增加。FTIR显微光谱显示脂肪酸和蛋白质的明显变化,与HFF+OV组更明显的组织病理学和肝功能受损相关。研究结果表明,HFF饮食和O. viverrini感染的相互作用通过增加肝损伤、炎症、纤维生成和代谢功能障碍来加剧MASLD的进展。这项研究强调了将营养和感染因素纳入肝脏疾病管理的重要性,特别是在阿片吸虫病常见的地区。
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引用次数: 0
New host record and redescription of Amblyomma cordiferum nymphs and larvae infesting Malaysian house rats (Rattus rattus diardii) in Peninsular Malaysia, with molecular evidence of Rickettsia, Borrelia, and Bartonella 马来西亚半岛马来西亚家鼠(Rattus Rattus diardii)感染的cordiblyomma amblyomum若虫和幼虫的新寄主记录和重新描述,并附有立克次体、疏螺旋体和巴尔通体的分子证据。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107496
Abdul Rahman Kazim , Dzulhelmi Muhammad Nasir , Tiong Kai Tan , Vinnie-Siow Wei Yin , Ariff Ateed Mohd Noh , Chong-Chin Heo , Van Lun Low
Nymphal and larval Amblyomma cordiferum ticks, a relatively rare species, were collected from the Malaysian house rat (Rattus rattus diardii) in Peninsular Malaysia. Redescription and molecular analysis of nymphs and larvae, based on the 12S rRNA, 16S rRNA, and COI genes, revealed divergence from A. cordiferum in Taiwan, suggesting that the Taiwanese tick specimens may represent a different taxon. Molecular analysis of the pathogens in these specimens revealed three sequences of Rickettsia closely related or identical to Rickettsia raoultii (99.71–100%), two sequences of relapsing fever Borrelia identical to Borrelia theileri, and four sequences of Bartonella identical to Bartonella phoceensis. This study also identifies a new host record for A. cordiferum in R. r. diardii and reports the first detection of Rickettsia, Borrelia, and Bartonella in this tick species.
在马来西亚半岛,从马来西亚家鼠(Rattus Rattus diardii)身上采集到一种较为罕见的蜱虫。利用12S rRNA、16S rRNA和COI基因对台湾蜱虫若虫和幼虫进行重新描述和分子分析,发现台湾蜱虫与台湾a . cordiferum存在差异,表明台湾蜱虫可能属于不同的分类群。病原分子分析显示,3个立克次体序列与拉乌尔立克次体密切相关或相同(99.71 ~ 100%),2个回归热伯氏疏螺旋体序列与他们的伯氏疏螺旋体相同,4个巴尔通体序列与phoceensis巴尔通体相同。本研究还在地鼠中发现了一种新的寄主记录,并报道了首次在地鼠中检测到立克次体、伯氏疏螺旋体和巴尔通体。
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引用次数: 0
Fauna of simuliids (Diptera: Simuliidae) of the Tormes River hydrographic basin in central-western Spain and their responses to abiotic factors in their habitats 西班牙中西部托尔梅斯河流域拟蝇科(双翅目:拟蝇科)区系及其对生境非生物因子的响应。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107516
David López-Peña , Manuel Salvador Portillo-Rubio , Eduardo Moisés García-Roger , Álvaro Lis-Cantín , José Vicente Falcó-Garí
Delving into knowing the blackfly (Diptera: Simuliidae) species composition of Spain and their ecological requirements is crucial, due to their instrumental role in natural food webs as intermediaries in the flow of energy in aquatic ecosystems, and because of the haematophagic behaviour displayed by females of several species. The present study has analysed the abundance and distribution of the larvae and pupae of blackfly species in 105 sampling stations located in lotic water bodies of the provinces of Ávila, Salamanca, and Zamora of the Tormes River basin. The study has allowed to identify 24 species: 17 from Ávila, 13 from Salamanca, and seven from Zamora, classified in three genera (Metacnephia, Prosimulium, and Simulium), and five subgenera (Boophthora, Eusimulium, Nevermannia, Simulium, Wilhelmia). The results include four first records for Ávila province: Simulium (Nevermannia) armoricanum Doby & David, 1961, Simulium (Nevermannia) cryophilum (Rubtsov, 1959), Simulium (Nevermannia) naturale Davies, 1966, and Simulium (Simulium) variegatum Meigen, 1818. Among them, S. armoricanum shows the widest elevational range (900-1650 m a.s.l.), S. naturale shows the widest water temperature range (6-15 °C), and S. cryophilum stands out for inhabiting very fast waters. Blackfly communities were best discriminated by elevation and temperature. Our analyses allowed us to estimate the optima and tolerance ranges of the described species, with S. argygreatum, S. reptans, P. latimucro and P. tomosvaryi standing out for their preference for altitudes above 1000 m a.s.l., although with wide tolerance ranges. In terms of temperature, these two Prosimulium species were best represented at the coldest waters.
深入了解西班牙黑蝇(双翅目:蚋科)的物种组成及其生态需求是至关重要的,因为它们在天然食物网中作为水生生态系统能量流动的媒介发挥着重要作用,而且由于一些物种的雌性表现出吸血行为。本研究分析了托尔梅斯河流域Ávila、萨拉曼卡省和萨莫拉省的105个采样点的黑蝇幼虫和蛹的丰度和分布情况。该研究已鉴定出24种:Ávila 17种,Salamanca 13种,Zamora 7种,分为3个属(Metacnephia, Prosimulium, Simulium)和5个亚属(Boophthora, Eusimulium, Nevermannia, Simulium, Wilhelmia)。结果包括Ávila省的四个第一记录:Simulium (Nevermannia) armoricanum Doby & David, 1961年,Simulium (Nevermannia) cryophilum (Rubtsov, 1959年),Simulium (Nevermannia) naturale Davies, 1966年和Simulium (Simulium) variegatum Meigen, 1818年。其中,S. armoricanum海拔范围最宽(900-1650 m a.s.l.), S. naturale水温范围最宽(6-15°C), S. cryophilum以生活在非常快的水域而突出。黑蝇群落的最佳区分依据是海拔和温度。我们的分析使我们能够估计所描述物种的最佳和耐受范围,其中S. argygreatum, S. reptans, P. latimucro和P. tomosvaryi在海拔1000 m以上的高度表现出偏好,尽管它们的耐受范围很广。就温度而言,这两种原藻在最冷的海水中最具代表性。
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引用次数: 0
A review of acaricides and their resistance mechanisms in hard ticks and control alternatives with synergistic agents 杀螨剂及其对硬蜱的抗性机制及协同药剂防治的研究进展。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107519
Jose Felix Rojas-Cabeza , Elena N. Moreno-Cordova , Jesus Fernando Ayala-Zavala , Adrian Ochoa-Teran , Daniel E. Sonenshine , Jesus G. Valenzuela , Rogerio R. Sotelo-Mundo
Ticks are significant ectoparasites that transmit a variety of pathogens, leading to serious human and animal diseases, including Lyme disease, Rocky Mountain spotted fever, anaplasmosis, and many others. The emergence of acaricide resistance in hard ticks presents a formidable challenge for public health and livestock management, exacerbated by the increasing incidence of tick-borne diseases and associated economic losses, estimated at $20 billion annually in the livestock sector alone. This review examines the mechanisms underlying acaricide resistance, focusing on genetic mutations, metabolic detoxification processes, and behavioral adaptations in tick populations. We detail the role of commercial acaricides in tick control while emphasizing the adverse effects of their overuse, which contributes to the development of resistant strains. Innovative control strategies are explored, including using pesticide synergists that enhance the efficacy of existing acaricides by targeting the tick's phosphagen system. Additionally, this review highlights the importance of understanding the synergistic interactions between various control methods, including non-chemical approaches such as personal protection measures and landscape management. The review concludes by underscoring the urgent need for novel acaricides with new modes of action and implementing regular monitoring practices to combat acaricide resistance effectively. Addressing these challenges is vital for the sustainable management of tick populations and protecting public health and livestock productivity.
蜱是重要的体外寄生虫,传播多种病原体,导致严重的人类和动物疾病,包括莱姆病、落基山斑疹热、无形体病和许多其他疾病。硬蜱中出现的杀螨剂耐药性给公共卫生和牲畜管理带来了巨大挑战,而蜱传疾病发病率的增加和相关的经济损失(仅畜牧业每年估计就高达200亿美元)加剧了这一挑战。本文综述了蜱虫种群中杀螨剂抗性的机制,重点是基因突变、代谢解毒过程和行为适应。我们详细介绍了商业杀螨剂在蜱虫控制中的作用,同时强调其过度使用的不利影响,这有助于抗性菌株的发展。探索了创新的控制策略,包括使用农药增效剂,通过针对蜱虫的磷系统来提高现有杀螨剂的功效。此外,本综述强调了了解各种控制方法之间协同作用的重要性,包括非化学方法,如个人保护措施和景观管理。该综述最后强调,迫切需要开发具有新作用方式的新型杀螨剂,并实施定期监测措施,以有效防治杀螨剂耐药性。应对这些挑战对于蜱虫种群的可持续管理以及保护公众健康和牲畜生产力至关重要。
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引用次数: 0
ToRCH pathogens-induced histopathological changes in placental tissues and associated post obstetric complications ToRCH病原体诱导的胎盘组织病理改变及相关的产后并发症。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107466
Maryam Naseem , Sanaullah Khan , Dalal Sulaiman Alshaya , Tawaf Ali Shah , Sarwat Noreen , Faiz Ur Rehman , Kotab A. Attia , Nuzhat Sultana
<div><h3>Background</h3><div>Maternal infections caused by the ToRCH complex, comprising <em>Toxoplasma gondii</em> (<em>T.gondii</em>), Rubella Virus (RV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV), are significant contributors to Bad Obstetric History (BOH). These infections can vertically transmit through the placental barrier, leading to complications in fetal development. This study investigates the histopathological changes induced by ToRCH pathogens in placental tissues and their association with post-obstetric complications in Pakistani women.</div></div><div><h3>Methodology</h3><div>A total of 83 women were enrolled in the study and divided into two groups: the Experimental group and the Control group. The Experimental group consisted of women with BOH and was further subdivided into two categories: Group A and Group B. Group A comprised women who experienced a recent miscarriage at the time of sample collection, while Group B consisted of women who gave birth normally at the time of sample collection. In contrast, the Control group included women with normal delivery and a normal obstetric history. To investigate ToRCH infections, venous blood samples were analyzed for ToRCH antibodies using enzyme-linked immunosorbent assay (ELISA). Additionally, small pieces of placental tissue collected after miscarriage or delivery were subjected to nucleic acid (NA) detection by conventional polymerase chain reaction (PCR) using ToRCH pathogen-specific primers. Histopathological examination of placental tissue was also performed to identify microscopic changes. The data obtained were analyzed using descriptive statistics (percentages) and chi-square tests to determine significant associations.</div></div><div><h3>Results</h3><div>The study revealed an overall seroprevalence of ToRCH pathogens of 57.83 % in the study population, with 53.01 % detected in the Experimental group and 4.81 % in the Control group. In the Experimental group, IgM and IgG antibodies were detected in 14.45 % and 37.34 % of participants, respectively, with 1.20 % testing positive for both. Notably, the Control group showed no IgM positivity, while IgG was detected in 4.81 %. Nucleic acid (NA) detection revealed ToRCH pathogens in 54.21 % of placental tissues from the Experimental group only. Specifically, antibodies for <em>T.gondii</em> and RV were most prevalent (19.27 % each), while NA detection was highest for RV, CMV, and HSV (14.45 % each). In contrast, the Control group showed minimal antibody detection, with only 2.40 % positivity for <em>T.gondii</em> and CMV each. Statistical analysis revealed significant differences in ToRCH infection (antibodies and NA) between study groups for CMV only (<em>p</em> > 0.05). Discrepancies between ToRCH antibodies and NA positivity were observed in 75.90 % of cases, with 71.08 % occurring in the Experimental group and 4.81 % in the Control group. Histopathological examination revealed chorionic villitis (CV) in 16.86 %
背景:由刚地弓形虫(T.gondii)、风疹病毒(RV)、巨细胞病毒(CMV)和单纯疱疹病毒(HSV)组成的ToRCH复合物引起的孕产妇感染是不良产科史(BOH)的重要因素。这些感染可通过胎盘屏障垂直传播,导致胎儿发育并发症。本研究调查了巴基斯坦妇女胎盘组织中ToRCH病原体引起的组织病理学变化及其与产后并发症的关系。方法:共有83名女性被纳入研究,并分为两组:实验组和对照组。实验组由患有BOH的妇女组成,并进一步分为两类:A组和B组。A组包括在收集样本时最近经历过流产的妇女,而B组包括在收集样本时正常分娩的妇女。相比之下,对照组包括正常分娩和正常产科史的妇女。采用酶联免疫吸附试验(ELISA)对静脉血样本进行ToRCH抗体检测。此外,利用ToRCH病原体特异性引物,对流产或分娩后收集的小块胎盘组织进行常规聚合酶链反应(PCR)核酸检测。同时对胎盘组织进行组织病理学检查,以确定显微镜下的变化。使用描述性统计(百分比)和卡方检验分析获得的数据,以确定显著相关性。结果:研究人群ToRCH病原菌血清总阳性率为57.83%,其中实验组为53.01%,对照组为4.81%。实验组中IgM和IgG抗体检出率分别为14.45%和37.34%,其中1.20%均为阳性。对照组未检出IgM阳性,IgG阳性率为4.81%。核酸(NA)检测仅实验组胎盘组织中就检出54.21%的ToRCH病原体。其中,弓形虫和RV抗体检出率最高(分别为19.27%),而RV、CMV和HSV抗体检出率最高(分别为14.45%)。相比之下,对照组抗体检出率最低,弓形虫和巨细胞病毒阳性率仅为2.40%。统计分析显示,仅CMV组间ToRCH感染(抗体和NA)差异有统计学意义(p < 0.05)。75.90%的病例ToRCH抗体与NA阳性不一致,其中实验组为71.08%,对照组为4.81%。组织病理学检查显示,实验组有16.86%的患者出现绒毛膜绒毛炎(CV);其中,感染弓形虫(4例)、RV(6例)、巨细胞病毒(CMV)和HSV(各2例)的妇女CV阳性率分别为10.84%和6.02%。既往最常见的BOH并发症为复发性自然流产(RSAs)(47.05%),其中21.87%为CV阳性。结论:本研究的关键结论是ToRCH病原体在研究人群中普遍存在,CV被确定为与BOH并发症(主要是RSAs)相关的主要组织病理学改变。这些结果强调了在经历BOH并发症的妇女中调查ToRCH感染的重要性。
{"title":"ToRCH pathogens-induced histopathological changes in placental tissues and associated post obstetric complications","authors":"Maryam Naseem ,&nbsp;Sanaullah Khan ,&nbsp;Dalal Sulaiman Alshaya ,&nbsp;Tawaf Ali Shah ,&nbsp;Sarwat Noreen ,&nbsp;Faiz Ur Rehman ,&nbsp;Kotab A. Attia ,&nbsp;Nuzhat Sultana","doi":"10.1016/j.actatropica.2024.107466","DOIUrl":"10.1016/j.actatropica.2024.107466","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Maternal infections caused by the ToRCH complex, comprising &lt;em&gt;Toxoplasma gondii&lt;/em&gt; (&lt;em&gt;T.gondii&lt;/em&gt;), Rubella Virus (RV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV), are significant contributors to Bad Obstetric History (BOH). These infections can vertically transmit through the placental barrier, leading to complications in fetal development. This study investigates the histopathological changes induced by ToRCH pathogens in placental tissues and their association with post-obstetric complications in Pakistani women.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methodology&lt;/h3&gt;&lt;div&gt;A total of 83 women were enrolled in the study and divided into two groups: the Experimental group and the Control group. The Experimental group consisted of women with BOH and was further subdivided into two categories: Group A and Group B. Group A comprised women who experienced a recent miscarriage at the time of sample collection, while Group B consisted of women who gave birth normally at the time of sample collection. In contrast, the Control group included women with normal delivery and a normal obstetric history. To investigate ToRCH infections, venous blood samples were analyzed for ToRCH antibodies using enzyme-linked immunosorbent assay (ELISA). Additionally, small pieces of placental tissue collected after miscarriage or delivery were subjected to nucleic acid (NA) detection by conventional polymerase chain reaction (PCR) using ToRCH pathogen-specific primers. Histopathological examination of placental tissue was also performed to identify microscopic changes. The data obtained were analyzed using descriptive statistics (percentages) and chi-square tests to determine significant associations.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The study revealed an overall seroprevalence of ToRCH pathogens of 57.83 % in the study population, with 53.01 % detected in the Experimental group and 4.81 % in the Control group. In the Experimental group, IgM and IgG antibodies were detected in 14.45 % and 37.34 % of participants, respectively, with 1.20 % testing positive for both. Notably, the Control group showed no IgM positivity, while IgG was detected in 4.81 %. Nucleic acid (NA) detection revealed ToRCH pathogens in 54.21 % of placental tissues from the Experimental group only. Specifically, antibodies for &lt;em&gt;T.gondii&lt;/em&gt; and RV were most prevalent (19.27 % each), while NA detection was highest for RV, CMV, and HSV (14.45 % each). In contrast, the Control group showed minimal antibody detection, with only 2.40 % positivity for &lt;em&gt;T.gondii&lt;/em&gt; and CMV each. Statistical analysis revealed significant differences in ToRCH infection (antibodies and NA) between study groups for CMV only (&lt;em&gt;p&lt;/em&gt; &gt; 0.05). Discrepancies between ToRCH antibodies and NA positivity were observed in 75.90 % of cases, with 71.08 % occurring in the Experimental group and 4.81 % in the Control group. Histopathological examination revealed chorionic villitis (CV) in 16.86 %","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"261 ","pages":"Article 107466"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Theranostics in the management of Acanthamoeba infections 棘阿米巴感染的治疗学。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107494
Ruqaiyyah Siddiqui , David Lloyd , Naveed Ahmed Khan
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引用次数: 0
ICOSL deficiency promotes M1 polarization to alleviate liver fibrosis in schistosomiasis mice ICOSL 缺乏会促进 M1 极化,从而缓解血吸虫病小鼠的肝纤维化。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107470
Lei Liu , Peng Wang , Shi-Qi Xie , Wen-Jie Pu , Jing Xu , Chao-Ming Xia
The expression of inducible co-stimulator ligand (ICOSL) on macrophage (Mφ) implies their ability to interact with inducible co-stimulator (ICOS)-expressing T cells, thereby modulating immune responses within the liver microenvironment. This study aimed to elucidate the mechanism underlying ICOS/ICOSL signaling in the regulation of Mφ polarization during Schistosomiasis-induced liver fibrosis. To investigate this, ICOSL-knock out (KO) and wildtype (WT) C57BL/6 mice were infected with Schistosoma japonicum (S. japonicum) to examine the dynamic changes in Mφ phenotype and observe the pathology alterations in the liver. There was significantly decreased expression of ICOSL both in monocytes of cirrhosis patients and the liver tissue of mice infected with S. japonicum. Furthermore, ICOSL-KO mice exhibited reduced liver granuloma formation and fibrosis during S. japonicum infection. Simultaneously, Mφ in ICOSL-KO mice polarized towards M1-type and induced apoptosis of hepatic stellate cells (HSCs). Overall, the blockade of ICOSL signaling could promote M1 polarization, induce HSCs apoptosis, and ameliorate hepatic fibrosis, suggesting that ICOSL may serve as a potential biomarker for prognosis and therapeutic target for schistosomiasis-induced hepatic fibrosis.
巨噬细胞(Mφ)上表达的诱导性共刺激因子配体(ICOSL)意味着它们能够与表达诱导性共刺激因子(ICOS)的T细胞相互作用,从而调节肝脏微环境中的免疫反应。本研究旨在阐明 ICOS/ICOSL 信号在血吸虫病诱导的肝纤维化过程中调控 Mφ 极化的机制。为此,研究人员用日本血吸虫(S. japonicum)感染ICOSL基因敲除(KO)和野生型(WT)C57BL/6小鼠,研究Mφ表型的动态变化,并观察肝脏的病理改变。在肝硬化患者的单核细胞和感染日本核虫小鼠的肝组织中,ICOSL的表达量都明显下降。此外,ICOSL-KO小鼠在感染日本痢疾杆菌时,肝脏肉芽肿的形成和纤维化都有所减少。同时,ICOSL-KO 小鼠体内的 Mφ 向 M1 型极化,并诱导肝星状细胞(HSCs)凋亡。总之,阻断ICOSL信号传导可促进M1极化、诱导造血干细胞凋亡并改善肝纤维化,这表明ICOSL可作为血吸虫病肝纤维化预后的潜在生物标志物和治疗靶点。
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引用次数: 0
Integrated surveillance for Oropouche Virus: Molecular evidence of potential urban vectors during an outbreak in the Brazilian Amazon Oropouche病毒综合监测:巴西亚马逊地区暴发期间潜在城市媒介的分子证据
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107487
Luiz Henrique Maciel Feitoza , Nadson Willian Felipe Gasparelo , Anne Caroline Alves Meireles , Flávia Geovana Fontineles Rios , Karolaine Santos Teixeira , Michelli Santos da Silva , Matheus de Araújo Paz , Tárcio Peixoto Roca , Hillquias Monteiro Moreira , Kamila Pereira de França , Deusilene Souza Vieira Dall'Acqua , Genimar Rebouças Julião , Jansen Fernandes de Medeiros
The Oropouche Virus (OROV) has Culicoides paraensis as its main vector in the urban cycle. Diagnoses of acute febrile cases and entomological collections were performed between January and April 2024 in the urban setting of Porto Velho, a city located in the western Brazilian Amazon. A total of 904 human samples were evaluated using RT-qPCR, of which 328 were positive for OROV. In addition, 3,950 insects were captured and Culex quinquefasciatus was the most abundant and predominant species. Seven mosquito samples showed amplification for OROV from pools of Cx. quinquefasciatus, Limatus durhamii and Aedes albopictus. Due to the first record of OROV in the two latter species, our findings can support new hypotheses about potential or bridge vectors. In turn, the high density of Cx. quinquefasciatus reinforces the supposition of this species as the main urban vector in the Amazon in the absence of C. paraensis. Complementary studies of vector competence and capacity are necessary for confirmation.
Oropouche病毒(OROV)在城市循环中以副库蚊(Culicoides paraensis)为主要媒介。2024年1月至4月期间,在巴西西部亚马逊地区韦柳港的城市环境中对急性发热病例进行了诊断并进行了昆虫学采集。RT-qPCR检测904份人标本,其中328份OROV阳性。捕获昆虫3950只,其中致倦库蚊数量最多,优势种最多。从Cx池中提取的7份蚊虫标本显示OROV扩增。致倦库蚊、杜哈伊蚊及白纹伊蚊。由于在后两个物种中首次记录了OROV,我们的发现可以支持关于潜在或桥梁载体的新假设。反过来,Cx的高密度。在没有拟倦库蚊的情况下,拟倦库蚊加强了该物种作为亚马逊地区主要城市媒介的假设。需要对病媒能力和能力进行补充性研究才能得到确认。
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引用次数: 0
Corrigendum to:“Molecular characteristics of Echinococcus multilocularis FABP1 and its regulatory functions on murine macrophages” [Acta Tropica, volume 255, article number 107247] 多房棘球绦虫FABP1的分子特性及其对小鼠巨噬细胞的调控作用[热带学报,255卷,文章号107247]的更正。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107495
Xiang Li, Peng Liao, Wenjing Zhou, Xinqi Yang, Bin Ye
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引用次数: 0
Immature survival and female longevity of Aedes aegypti under natural winter conditions in the temperate region of Argentina 阿根廷温带地区冬季自然条件下埃及伊蚊的未成年存活率和雌性寿命。
IF 2.1 3区 医学 Q2 PARASITOLOGY Pub Date : 2025-01-01 DOI: 10.1016/j.actatropica.2024.107507
Cristian M. Di Battista , Raúl E. Campos , Sylvia Fischer
The performance of Aedes aegypti was evaluated under natural winter nutritional and thermal conditions in the temperate region of Argentina. Immature stages were reared using leaf litter as a food source. The rearing was structured in three cohorts, the first started in late-fall, the second in early-winter and the last in mid-winter, and in each cohort two treatments were arranged according to solar exposure (sun and shade). The mean monthly temperature during the experiment ranged from 10.1 °C in July to 14.3 °C in the early days of October. Survival in the experiment was low (average 16.2 %), with a maximum (33 %) in mid-winter cohort. Development time from first instar larva to adult emergence varied between 24 and 103 days, was affected by cohort, treatment and their interaction, and showed an inverse relationship with temperature, particularly in the sun treatment. Individuals from the sun treatments of mid-winter and late-fall cohorts had shorter development times. Adult longevity was very low and was only affected by sex, being higher in females (4.5 days) than in males (3.1 days). The results of this work suggest that although immature stages may complete their development and reach the adult stage during the winter in temperate Argentina, females may not survive long enough to reproduce successfully.
在阿根廷温带地区,对埃及伊蚊在冬季自然营养和热条件下的表现进行了评价。未成熟阶段以落叶为食物来源饲养。饲养分为三个队列,第一组在深秋开始,第二组在初冬开始,最后一组在仲冬开始,每个队列中根据日照(阳光和阴凉处)安排两个处理。试验期间的月平均气温为7月的10.1℃~ 10月上旬的14.3℃。实验中生存率较低(平均16.2%),冬季中期生存率最高(33%)。从1龄幼虫到成虫羽化的发育时间在24 ~ 103 d之间,受种群、处理及其交互作用的影响,与温度呈反比关系,特别是在阳光处理下。中冬和晚秋日照组的个体发育时间较短。成虫寿命很低,仅受性别影响,雌性成虫寿命(4.5天)高于雄性成虫寿命(3.1天)。这项工作的结果表明,在温带的阿根廷,尽管未成熟的阶段可能在冬季完成发育并达到成年阶段,但雌性可能无法存活足够长的时间来成功繁殖。
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