Pub Date : 2023-11-01Epub Date: 2023-11-29DOI: 10.3114/fuse.2023.12.14
O Miettinen, J Vlasák, E Larsson, J Vlasák, J S S Seelan, Hernawati, Q Levicky, K-H Larsson, V Spirin
Cerrenaceae is a small family of polypores and hydnoid fungi in the order Polyporales (Basidiomycota). The family consists of white-rot fungi, some of which are serious tree pathogens. Combining morphological evidence with a phylogenetic dataset of six genetic markers, we revise generic concepts in the family and propose a seven-genus classification system for the family. Two genera are introduced as new: the monotypic Acanthodontia for Radulodon cirrhatinus, and Lividopora for the Rigidoporus vinctus complex. We re-introduce the name Somion for the Spongipellis delectans complex. Other recognized genera in the family are Cerrena, Irpiciporus, Pseudolagarobasidium, and Radulodon. New species introduced are Irpiciporus branchiformis from Tanzania, Lividopora armeniaca, and L. facilis from Southeast Asia, and Somion strenuum from East Asia. We provide nomenclatural comments on all the names combined to the above Cerrenaceae genera and typify Cerrena unicolor, C. zonata, Polyporus carneopallens (= L. vincta), Somion occarium, and S. unicolor. The genus Hyphoradulum belongs to Cystostereaceae (Agaricales), and we transfer the type species H. conspicuum to Crustomyces. Our study highlights the importance of integrating different basidiocarp types in analyses when revising genus classification in macrofungi. Citation: Miettinen O, Vlasák J, Larsson E, Vlasák J Jr., Seelan JSS, Hernawati, Levicky Q, Larsson K-H, Spirin V (2023). A revised genus-level classification for Cerrenaceae (Polyporales, Agaricomycetes). Fungal Systematics and Evolution12: 271-322. doi: 10.3114/fuse.2023.12.14.
Cerrenaceae 是多孔菌目（担子菌纲）中的一个小家族，由多孔菌和水螅菌组成。该科由白腐真菌组成，其中一些是严重的树木病原体。结合形态学证据和由六个遗传标记组成的系统发育数据集，我们修订了该科的属概念，并提出了该科的七属分类系统。我们引入了两个新属：Radulodon cirrhatinus 的单型属 Acanthodontia 和 Rigidoporus vinctus 复合属 Lividopora。我们为 Spongipellis delectans 复属重新引入了 Somion 这个名称。该科中其他公认的属是 Cerrena、Irpiciporus、Pseudolagarobasidium 和 Radulodon。引入的新物种有坦桑尼亚的 Irpiciporus branchiformis、东南亚的 Lividopora armeniaca 和 L. facilis 以及东亚的 Somion strenuum。我们对上述 Cerrenaceae 属的所有合并名称进行了命名注释，并对 Cerrena unicolor、C. zonata、Polyporus carneopallens（= L. vincta）、Somion occarium 和 S. unicolor 进行了分类。Hyphoradulum属属于子囊菌科（姬松茸属），我们将模式种H. conspicuum归入Cryustomces。我们的研究强调了在修订大型真菌的属分类时将不同基生果实类型纳入分析的重要性。引用：Miettinen O, Vlasák J, Larsson E, Vlasák J Jr., Seelan JSS, Hernawati, Levicky Q, Larsson K-H, Spirin V (2023).修订的 Cerrenaceae（多孔菌目，姬松茸科）属级分类。Fungal Systematics and Evolution 12: 271-322. doi: 10.3114/fuse.2023.12.14.
{"title":"A revised genus-level classification for <i>Cerrenaceae</i> (<i>Polyporales, Agaricomycetes</i>).","authors":"O Miettinen, J Vlasák, E Larsson, J Vlasák, J S S Seelan, Hernawati, Q Levicky, K-H Larsson, V Spirin","doi":"10.3114/fuse.2023.12.14","DOIUrl":"10.3114/fuse.2023.12.14","url":null,"abstract":"<p><p><b></b> <i>Cerrenaceae</i> is a small family of polypores and hydnoid fungi in the order <i>Polyporales</i> (<i>Basidiomycota</i>). The family consists of white-rot fungi, some of which are serious tree pathogens. Combining morphological evidence with a phylogenetic dataset of six genetic markers, we revise generic concepts in the family and propose a seven-genus classification system for the family. Two genera are introduced as new: the monotypic <i>Acanthodontia</i> for <i>Radulodon cirrhatinus</i>, and <i>Lividopora</i> for the <i>Rigidoporus vinctus</i> complex. We re-introduce the name <i>Somion</i> for the <i>Spongipellis delectans</i> complex. Other recognized genera in the family are <i>Cerrena, Irpiciporus, Pseudolagarobasidium</i>, and <i>Radulodon</i>. New species introduced are <i>Irpiciporus branchiformis</i> from Tanzania<i>, Lividopora armeniaca</i>, and <i>L. facilis</i> from Southeast Asia, and <i>Somion strenuum</i> from East Asia. We provide nomenclatural comments on all the names combined to the above <i>Cerrenaceae</i> genera and typify <i>Cerrena unicolor</i>, <i>C. zonata</i>, <i>Polyporus carneopallens</i> (= <i>L. vincta</i>), <i>Somion occarium</i>, and <i>S. unicolor</i>. The genus <i>Hyphoradulum</i> belongs to <i>Cystostereaceae</i> (<i>Agaricales</i>), and we transfer the type species <i>H. conspicuum</i> to <i>Crustomyces</i>. Our study highlights the importance of integrating different basidiocarp types in analyses when revising genus classification in macrofungi. <b>Citation:</b> Miettinen O, Vlasák J, Larsson E, Vlasák J Jr., Seelan JSS, Hernawati, Levicky Q, Larsson K-H, Spirin V (2023). A revised genus-level classification for <i>Cerrenaceae</i> (<i>Polyporales</i>, <i>Agaricomycetes</i>). <i>Fungal Systematics and Evolution</i> <b>12</b>: 271-322. doi: 10.3114/fuse.2023.12.14.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"12 ","pages":"271-322"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-01Epub Date: 2023-11-22DOI: 10.3114/fuse.2023.12.13
A Suija, R T McMullin, P Lõhmus
The globally distributed genus Trichaptum is one of the most species-rich among polypores in terms of hosting other fungi. Among Trichaptum-associates, there is a group of mazaediate lichenized fungi (Coniocybomycetes, Ascomycota) that previously had an uncertain phylogenetic position. DNA sequences - mitochondrial small subunit (mtSSU), nuclear large subunit rDNA (nuLSU), and internal transcribed spacer (ITS) - were obtained from 29 specimens collected from Europe and North America. Maximum likelihood and Bayesian inference analyses of these three gene loci were used to infer phylogenetic position and relationships among lineages. Statistical tests were used to find which phenotypical characteristics distinguish species. The molecular sequence data provide evidence that the fungicolous specimens form a distinct lineage within Coniocybomycetes sister to the combined clade of Chaenotheca s. lat. and Sclerophora. Considering its phylogenetic placement and strict specialization, we describe a new genus - Chaenotricha. This fungicolous lineage contains three species based on molecular characteristics. Morphological characters mostly overlap except for spore size and stalk length of apothecia. We provide a new combination, Chaenotricha obscura, for the only previously described species for which we designate an epitype, and introduce a new species - Chaenotricha cilians. The third lineage remains undescribed because of a small sample size, which did not allow us to clearly delineate species boundaries. Citation: Suija A, McMullin RT, Lõhmus P (2023). A phylogenetic assessment of a fungicolous lineage in Coniocybomycetes: Chaenotricha, a new genus of Trichaptum-inhabiting species. Fungal Systematics and Evolution 12: 255-269. doi: 10.3114/fuse.2023.12.13.
分布于全球的毛癣菌属是多孔菌中寄生其他真菌种类最丰富的属之一。在 Trichaptum 的同属真菌中，有一组马扎地衣化真菌（子囊菌目，子囊菌科），它们以前的系统发育位置并不确定。从欧洲和北美采集的 29 个标本中获得了 DNA 序列--线粒体小亚基（mtSSU）、核大亚基 rDNA（nuLSU）和内部转录间隔（ITS）。对这三个基因位点的最大似然法和贝叶斯推断分析被用来推断系统发育位置和各系之间的关系。统计检验用于确定哪些表型特征可区分物种。分子序列数据提供的证据表明，嗜真菌标本在壳斗科（Coniocybomycetes）中形成了一个独特的支系，是Chaenotheca s. lat.和Sclerophora联合支系的姐妹支系。考虑到其系统发育的位置和严格的特化，我们描述了一个新属--Chaenotricha。根据分子特征，该真菌系包含三个物种。除孢子大小和叶柄长度外，形态特征大多重叠。我们提供了一个新的组合，即 Chaenotricha obscura，这是之前唯一描述过的物种，我们为其指定了一个外型，并引入了一个新种--Chaenotricha cilians。由于样本量较小，我们无法明确划分物种界限，因此第三系仍未被描述。引用：Suija A, McMullin RT, Lõhmus P (2023)。Coniocybomycetes 中一个菌系的系统发育评估：Chaenotricha, a new genus of Trichaptum-inhabiting species.Fungal Systematics and Evolution 12: 255-269. doi: 10.3114/fuse.2023.12.13.
{"title":"A phylogenetic assessment of a fungicolous lineage in <i>Coniocybomycetes: Chaenotricha</i>, a new genus of <i>Trichaptum</i>-inhabiting species.","authors":"A Suija, R T McMullin, P Lõhmus","doi":"10.3114/fuse.2023.12.13","DOIUrl":"10.3114/fuse.2023.12.13","url":null,"abstract":"<p><p><b></b> The globally distributed genus <i>Trichaptum</i> is one of the most species-rich among polypores in terms of hosting other fungi. Among Trichaptum-associates, there is a group of mazaediate lichenized fungi <i>(Coniocybomycetes, Ascomycota)</i> that previously had an uncertain phylogenetic position. DNA sequences - mitochondrial small subunit (mtSSU), nuclear large subunit rDNA (nuLSU), and internal transcribed spacer (ITS) - were obtained from 29 specimens collected from Europe and North America. Maximum likelihood and Bayesian inference analyses of these three gene loci were used to infer phylogenetic position and relationships among lineages. Statistical tests were used to find which phenotypical characteristics distinguish species. The molecular sequence data provide evidence that the fungicolous specimens form a distinct lineage within <i>Coniocybomycetes</i> sister to the combined clade of <i>Chaenotheca s. lat</i>. and <i>Sclerophora</i>. Considering its phylogenetic placement and strict specialization, we describe a new genus - <i>Chaenotricha</i>. This fungicolous lineage contains three species based on molecular characteristics. Morphological characters mostly overlap except for spore size and stalk length of apothecia. We provide a new combination, <i>Chaenotricha obscura</i>, for the only previously described species for which we designate an epitype, and introduce a new species - <i>Chaenotricha cilians</i>. The third lineage remains undescribed because of a small sample size, which did not allow us to clearly delineate species boundaries. <b>Citation</b>: Suija A, McMullin RT, Lõhmus P (2023). A phylogenetic assessment of a fungicolous lineage in <i>Coniocybomycetes: Chaenotricha</i>, a new genus of <i>Trichaptum-inhabiting</i> species. <i>Fungal Systematics and Evolution</i> 12: 255-269. doi: 10.3114/fuse.2023.12.13.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"12 ","pages":"255-269"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-01-23DOI: 10.3114/fuse.2023.11.01
A Ohmaki, I Okane, P W Crous, G J M Verkley
A nectrioid fungus forming a pinkish colony with mainly solitary phialides producing ellipsoid, aseptate conidia in mucoid packets was isolated from Dirinaria applanata. Our taxonomic study based on morphology and phylogenetic analysis using ITS rDNA sequences revealed that the isolates represented a member of the genus Cylindromonium. Based on further morphological examination, nucleotide sequence comparison, and phylogenetic analysis based on LSU rDNA, tef1, and rpb2 in addition to the phylogenetic analysis using the ITS rDNA sequences, the fungus from Dirinaria represents a new species, which is described here as Cylindromonium dirinariaesp. nov. Furthermore, inoculation experiments revealed that this species can also produce perithecia when inoculated on the host lichen in laboratory environments. Citation: Ohmaki A, Okane I, Crous PW, Verkley GJM (2023). Cylindromonium dirinariaesp. nov. (Ascomycota, Hypocreales), a new nectrioid lichenicolous species on Dirinariaapplanata in Japan. Fungal Systematics and Evolution11: 1-10. doi: 10.3114/fuse.2023.11.01.
从 Dirinaria applanata 中分离出了一种蜜腺真菌，其菌落呈粉红色，主要是单生分生孢子，产生椭圆形、无菌的分生孢子，呈粘液状。我们根据形态学和使用 ITS rDNA 序列进行的系统发育分析进行了分类研究，结果表明分离物代表了圆柱孢属的一个成员。根据进一步的形态学检查、核苷酸序列比较，以及基于 LSU rDNA、tef1 和 rpb2 的系统发育分析和基于 ITS rDNA 序列的系统发育分析，来自 Dirinaria 的真菌代表了一个新种，在此描述为 Cylindromonium dirinariae sp.此外，接种实验表明，在实验室环境中，接种到寄主地衣上，该物种也能产生栖息孢子。引用：Ohmaki A, Okane I：Ohmaki A, Okane I, Crous PW, Verkley GJM (2023).(Ascomycota, Hypocreales), a new nectrioid lichenicolous species on Dirinaria applanata in Japan.Fungal Systematics and Evolution 11: 1-10. doi: 10.3114/fuse.2023.11.01.
{"title":"<i>Cylindromonium dirinariae sp. nov</i>. (<i>Ascomycota, Hypocreales</i>), a new nectrioid lichenicolous species on <i>Dirinaria applanata</i> in Japan.","authors":"A Ohmaki, I Okane, P W Crous, G J M Verkley","doi":"10.3114/fuse.2023.11.01","DOIUrl":"10.3114/fuse.2023.11.01","url":null,"abstract":"<p><p>A nectrioid fungus forming a pinkish colony with mainly solitary phialides producing ellipsoid, aseptate conidia in mucoid packets was isolated from <i>Dirinaria applanata</i>. Our taxonomic study based on morphology and phylogenetic analysis using ITS rDNA sequences revealed that the isolates represented a member of the genus <i>Cylindromonium</i>. Based on further morphological examination, nucleotide sequence comparison, and phylogenetic analysis based on LSU rDNA, <i>tef1</i>, and <i>rpb2</i> in addition to the phylogenetic analysis using the ITS rDNA sequences, the fungus from <i>Dirinaria</i> represents a new species, which is described here as <i>Cylindromonium dirinariae</i> <i>sp. nov</i>. Furthermore, inoculation experiments revealed that this species can also produce perithecia when inoculated on the host lichen in laboratory environments. <b>Citation:</b> Ohmaki A, Okane I, Crous PW, Verkley GJM (2023). <i>Cylindromonium dirinariae</i> <i>sp. nov</i>. (<i>Ascomycota, Hypocreales</i>), a new nectrioid lichenicolous species on <i>Dirinaria</i> <i>applanata</i> in Japan. <i>Fungal Systematics and Evolution</i> <b>11</b>: 1-10. doi: 10.3114/fuse.2023.11.01.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"11 ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9842446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.3114/fuse.2023.11.06-supp
Supplementary information: Supplementary Table Supplementary Table: All Helvella specimens investigated for this study where least one Sanger sequence is available. All specimens are from the nordic countries. Which sequences that are available for each speciemens is marked with GenBank number or marker name. All sequences are available in alignments submitted to DataverseNO repository.The sequences with GenBank accession numbers can also be found in GenBank.
{"title":"Additions to the knowledge of the genus Helvella in Europe. New records and de novo description of five species from the Nordic region","authors":"","doi":"10.3114/fuse.2023.11.06-supp","DOIUrl":"https://doi.org/10.3114/fuse.2023.11.06-supp","url":null,"abstract":"Supplementary information:<br /> Supplementary Table Supplementary Table: All Helvella specimens investigated for this study where least one Sanger sequence is available. All specimens are from the nordic countries. Which sequences that are available for each speciemens is marked with GenBank number or marker name. All sequences are available in alignments submitted to DataverseNO repository.The sequences with GenBank accession numbers can also be found in GenBank.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.3114/fuse.2023.11.03-supp
Suppplementary information: Fig. S1. Phylogenetic tree obtained by ML analyses using ITS sequence data of Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria stevensii (CBS 112553) and B. obtusa (CMW 8232). Ex-type strains are indicated in bold. Isolates from the present study are marked in blue. Fig. S2. Phylogenetic tree obtained by ML analyses using LSU sequence data of the Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria stevensii (CBS 112553). Ex-type strains are indicated in bold. Isolates from the present study are marked in blue. Fig. S3. Phylogenetic tree obtained by ML analyses using tef sequence data of the Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria obtusa (CMW 8232). Ex-type strains are indicated in bold. Isolates from the present study are marked in blue. Fig. S4. Phylogenetic tree obtained by ML analyses using act sequence data of the Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria stevensii (CBS 112553) and B. obtusa (CMW 8232). Extype strains are indicated in bold. Isolates from the present study are marked in blue. Table S1. Morphological comparison of species in the P. concentrica species complex.
{"title":"Phyllosticta rizhaoensis sp. nov. causing leaf blight of Ophiopogon japonicus in China","authors":"","doi":"10.3114/fuse.2023.11.03-supp","DOIUrl":"https://doi.org/10.3114/fuse.2023.11.03-supp","url":null,"abstract":"Suppplementary information:<br /> Fig. S1. Phylogenetic tree obtained by ML analyses using ITS sequence data of Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria stevensii (CBS 112553) and B. obtusa (CMW 8232). Ex-type strains are indicated in bold. Isolates from the present study are marked in blue.<br /> Fig. S2. Phylogenetic tree obtained by ML analyses using LSU sequence data of the Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria stevensii (CBS 112553). Ex-type strains are indicated in bold. Isolates from the present study are marked in blue.<br /> Fig. S3. Phylogenetic tree obtained by ML analyses using tef sequence data of the Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria obtusa (CMW 8232). Ex-type strains are indicated in bold. Isolates from the present study are marked in blue.<br /> Fig. S4. Phylogenetic tree obtained by ML analyses using act sequence data of the Phyllosticta concentrica species complex. Numbers above the branches indicate ML bootstraps (left, ML ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.9). The tree is rooted with Botryosphaeria stevensii (CBS 112553) and B. obtusa (CMW 8232). Extype strains are indicated in bold. Isolates from the present study are marked in blue.<br /> Table S1. Morphological comparison of species in the P. concentrica species complex.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The genus Ducellieria ( Ducellieriaceae ) contains three species ( D. chodatii , D. tricuspidata , D. corcontica ), and a single variety ( D. chodatii var. armata ) of obligate endobiotic pollen parasites. These organisms have been first assigned to the green alga genus Coelastrum , as they form very similar spherical structures, but the observation of heterokont zoospores has led to their reclassification to the phylum Oomycota . However, despite their widespread nature, these organisms are only known from their descriptive morphology, and life cycle traits of some species still remain incompletely known. Only the type species, D. chodatii , has been rediscovered several times, but the phylogeny of the genus remains unresolved, since none of its species has been studied for their molecular phylogeny. At present the genus is still included in some algal databases. To clarify the evolutionary affiliation of Ducellieria , efforts were undertaken to isolate D. chodatii from pollen grains, to infer its phylogenetic placement based on nrSSU sequences. By targeted isolation, the pollen endoparasitoid was rediscovered from three lakes in Germany (Mummelsee, Okertalsperre, Knappensee). Apart from the typical coelastrum-like spheroids, oomycetes sporulating directly from pollen grains in a lagenidium-like fashion were observed, and molecular sequences of both types of oomycetes were obtained. Phylogenetic reconstruction revealed that coelastrum-like and lagenidium-like forms are unrelated, with the former embedded within the deep branching early-diverging lineages, and the later stage forming a distinct clade in Peronosporales . Consequently, the life cycle of D. chodatii needs careful revision using single-spore isolates of the species, to infer if previous lifecycle reconstructions that involve various different thallus types are stages of a single species or potentially of several ones.
Ducellieria属(Ducellieriaceae)包含三种(D. chodatii, D. tricuspidata, D. corcontica)和一种专性内源花粉寄生虫(D. chodatii var. armata)。由于它们形成非常相似的球形结构，这些生物最初被分配到绿藻属腔藻，但对异源游动孢子的观察导致它们被重新分类到卵菌门。然而，尽管这些生物分布广泛，但人们只从它们的描述形态中了解它们，一些物种的生命周期特征仍然不完全清楚。只有模式种D. chodatii被重新发现了几次，但该属的系统发育仍然没有得到解决，因为它的物种都没有被研究过它们的分子系统发育。目前，该属仍包括在一些藻类数据库中。为了明确Ducellieria的进化关系，研究人员从花粉粒中分离出chodatii，并根据nrSSU序列推断其系统发育位置。通过定向分离，在德国的三个湖泊(Mummelsee, Okertalsperre, Knappensee)中重新发现了花粉内生寄生虫。除了典型的腔乳状球体外，还观察到直接从花粉粒中以类似于lagenidia的方式产孢的卵菌，并获得了这两种类型卵菌的分子序列。系统发育重建显示，腔乳状体和lagenidim状体是不相关的，前者嵌入在深分支的早期分支谱系中，而后者在Peronosporales中形成一个独特的分支。因此，需要使用该物种的单孢子分离物仔细修改D. chodatii的生命周期，以推断先前涉及各种不同菌体类型的生命周期重建是单一物种的阶段还是可能是多个物种的阶段。
{"title":"Ducellieriales ord. nov. and evidence for a novel clade of endobiotic pollen-infecting “lagenidiaceous” Peronosporomycetes","authors":"A.T. Buaya, M. Thines","doi":"10.3114/fuse.2023.12.12","DOIUrl":"https://doi.org/10.3114/fuse.2023.12.12","url":null,"abstract":"The genus Ducellieria ( Ducellieriaceae ) contains three species ( D. chodatii , D. tricuspidata , D. corcontica ), and a single variety ( D. chodatii var. armata ) of obligate endobiotic pollen parasites. These organisms have been first assigned to the green alga genus Coelastrum , as they form very similar spherical structures, but the observation of heterokont zoospores has led to their reclassification to the phylum Oomycota . However, despite their widespread nature, these organisms are only known from their descriptive morphology, and life cycle traits of some species still remain incompletely known. Only the type species, D. chodatii , has been rediscovered several times, but the phylogeny of the genus remains unresolved, since none of its species has been studied for their molecular phylogeny. At present the genus is still included in some algal databases. To clarify the evolutionary affiliation of Ducellieria , efforts were undertaken to isolate D. chodatii from pollen grains, to infer its phylogenetic placement based on nrSSU sequences. By targeted isolation, the pollen endoparasitoid was rediscovered from three lakes in Germany (Mummelsee, Okertalsperre, Knappensee). Apart from the typical coelastrum-like spheroids, oomycetes sporulating directly from pollen grains in a lagenidium-like fashion were observed, and molecular sequences of both types of oomycetes were obtained. Phylogenetic reconstruction revealed that coelastrum-like and lagenidium-like forms are unrelated, with the former embedded within the deep branching early-diverging lineages, and the later stage forming a distinct clade in Peronosporales . Consequently, the life cycle of D. chodatii needs careful revision using single-spore isolates of the species, to infer if previous lifecycle reconstructions that involve various different thallus types are stages of a single species or potentially of several ones.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"133 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135057245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.3114/fuse.2023.11.02-supp
Supplementary information: Fig. S1 .Maximum likelihood tree with rapid bootstrap values. The species names are followed by voucher or strain number and country of origin. Species names in red are from Benin. Fig. S2. Maximum likelihood (ML) analysis of the genus Microporus with rapid bootstrap values based on the combined ITS-LSU-Tef dataset.Newly generated sequences highlighted in red. The sequence namesare followed by voucher or strain number and country of origin.Table S1. Taxon names and GenBank accession numbers of somesequences used in this study.
{"title":"New records and barcode sequence data of wood-inhabiting polypores in Benin with notes on their phylogenetic placements and distribution","authors":"","doi":"10.3114/fuse.2023.11.02-supp","DOIUrl":"https://doi.org/10.3114/fuse.2023.11.02-supp","url":null,"abstract":"Supplementary information:<br /> Fig. S1 .Maximum likelihood tree with rapid bootstrap values. The species names are followed by voucher or strain number and country of origin. Species names in red are from Benin.<br /> Fig. S2. Maximum likelihood (ML) analysis of the genus Microporus with rapid bootstrap values based on the combined ITS-LSU-Tef dataset.Newly generated sequences highlighted in red. The sequence namesare followed by voucher or strain number and country of origin.Table S1. Taxon names and GenBank accession numbers of somesequences used in this study.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.3114/fuse.2023.11.08-supp
Supplementary information: Supplementary File 1. Sequence alignment of concatenated dataset.The full length of dataset is 1153 bp. Sections: cox2 positions 1–437, nrLSU positions 438–1153. Supplementary File 2. Phylogenetic reconstructions (in Minimum Evolution) for the individual loci. Bootstrap support values from Minimum Evolution and Maximum Likelihood, and posterior probabilities from Bayesian Inference are given on the branches next to the nodes in the respective order. A minus sign denotes an alternate but not highly supported topology (BS < 80 % or PP < 0.98). An “x” represents alternate highly supported topology (BS > 80 % or PP > 0.98). The isolates of Rhipidium interruptum derived from this study are marked in bold. The scale bars represent the number of substitutions per site.
{"title":"Adding a missing piece to the puzzle of oomycete phylogeny: the placement of Rhipidium interruptum (Rhipidiaceae)","authors":"","doi":"10.3114/fuse.2023.11.08-supp","DOIUrl":"https://doi.org/10.3114/fuse.2023.11.08-supp","url":null,"abstract":"Supplementary information:<br /> Supplementary File 1. Sequence alignment of concatenated dataset.The full length of dataset is 1153 bp. Sections: cox2 positions 1–437, nrLSU positions 438–1153.<br /> Supplementary File 2. Phylogenetic reconstructions (in Minimum Evolution) for the individual loci. Bootstrap support values from Minimum Evolution and Maximum Likelihood, and posterior probabilities from Bayesian Inference are given on the branches next to the nodes in the respective order. A minus sign denotes an alternate but not highly supported topology (BS < 80 % or PP < 0.98). An “x” represents alternate highly supported topology (BS > 80 % or PP > 0.98). The isolates of Rhipidium interruptum derived from this study are marked in bold. The scale bars represent the number of substitutions per site.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H.S. Ferdinandez, D.S. Manamgoda, D. Udayanga, M.S. Munasinghe, L.A. Castlebury
Despite being a small island, Sri Lanka is rich in fungal diversity. Most of the fungi from Sri Lanka have been identified as pathogens of vegetables, fruits, and plantation crops to date. The pleosporalean genus Curvularia ( Dothideomycetes ) includes phytopathogenic, saprobic, endophytic, and human/animal opportunistic pathogenic fungal species. The majority of the plant-associated Curvularia species are known from poaceous hosts. During the current study, 22 geographical locations of the country were explored and collections were made from 10 different poaceous hosts. Morphology and molecular phylogeny based on three loci, including nuclear internal transcribed spacers 1 and 2 with 5.8S nrDNA (ITS), glyceraldehyde-3-phosphate dehydrogenase ( gapdh ), and translation elongation factor 1-α ( tef1 ) supported the description of two new species of fungi described herein as C. aurantia sp. nov. and C. vidyodayana sp. nov. Moreover, novel host-fungal association records for C. chiangmaiensis , C. falsilunata , C. lonarensis , C. plantarum , and C. pseudobrachyspora are updated herein. In addition, five species within the genus Curvularia , viz ., C. asiatica , C. geniculata , C. lunata , C. muehlenbeckiae , and C. verruculosa represent new records of fungi from Sri Lanka.
{"title":"Molecular phylogeny and morphology reveal two new graminicolous species, Curvularia aurantia sp. nov. and C. vidyodayana sp. nov. with new records of Curvularia spp. from Sri Lanka","authors":"H.S. Ferdinandez, D.S. Manamgoda, D. Udayanga, M.S. Munasinghe, L.A. Castlebury","doi":"10.3114/fuse.2023.12.11","DOIUrl":"https://doi.org/10.3114/fuse.2023.12.11","url":null,"abstract":"Despite being a small island, Sri Lanka is rich in fungal diversity. Most of the fungi from Sri Lanka have been identified as pathogens of vegetables, fruits, and plantation crops to date. The pleosporalean genus Curvularia ( Dothideomycetes ) includes phytopathogenic, saprobic, endophytic, and human/animal opportunistic pathogenic fungal species. The majority of the plant-associated Curvularia species are known from poaceous hosts. During the current study, 22 geographical locations of the country were explored and collections were made from 10 different poaceous hosts. Morphology and molecular phylogeny based on three loci, including nuclear internal transcribed spacers 1 and 2 with 5.8S nrDNA (ITS), glyceraldehyde-3-phosphate dehydrogenase ( gapdh ), and translation elongation factor 1-α ( tef1 ) supported the description of two new species of fungi described herein as C. aurantia sp. nov. and C. vidyodayana sp. nov. Moreover, novel host-fungal association records for C. chiangmaiensis , C. falsilunata , C. lonarensis , C. plantarum , and C. pseudobrachyspora are updated herein. In addition, five species within the genus Curvularia , viz ., C. asiatica , C. geniculata , C. lunata , C. muehlenbeckiae , and C. verruculosa represent new records of fungi from Sri Lanka.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135800692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Downy mildew is one of the most important diseases of commercial sunflower and other Asteraceae hosts, including ornamental Rudbeckia . Plasmopara halstedii has historically been identified as the causal agent of this disease, considered a complex of species affecting nearly 35 genera in various tribes. However, with the use of molecular DNA characters for phylogenetic studies, distinct lineages of P. halstedii in the Asteraceae have been identified, confirmed as distinct or segregated as new species. During August of 2022, a downy mildew was observed on potted Echinacea purpurea grown in a retail greenhouse in Jefferson County, Wisconsin, USA. Phylogenetic analyses of the cytochrome c oxidase subunit 2 ( cox2 ) and nuclear large subunit ribosomal RNA (nc LSU rDNA) gene regions indicated these Plasmopara sp. isolates are not conspecific with P. halstedii . Based on phylogenetic evidence and new host association, the Plasmopara isolates from E. purpurea are here described as Plasmopara echinaceae . Diagnostic morphological characters for this new species were not observed when compared with other isolates of P. halstedii or other Plasmopara species found on Asteraceae hosts, and therefore a list of species-specific substitutions in the cox2 region are provided as diagnostic characters. As this study corresponds to the first observation of downy mildew in cone flowers, it is recommended to follow the required disease prevention guidelines to prevent outbreaks and the establishment of this plant pathogen in production sites.
{"title":"Plasmopara echinaceae, a new species of downy mildew affecting cone flowers (Echinacea purpurea) in the United States","authors":"C. Salgado-Salazar, M.K. Romberg, B. Hudelson","doi":"10.3114/fuse.2023.12.10","DOIUrl":"https://doi.org/10.3114/fuse.2023.12.10","url":null,"abstract":"Downy mildew is one of the most important diseases of commercial sunflower and other Asteraceae hosts, including ornamental Rudbeckia . Plasmopara halstedii has historically been identified as the causal agent of this disease, considered a complex of species affecting nearly 35 genera in various tribes. However, with the use of molecular DNA characters for phylogenetic studies, distinct lineages of P. halstedii in the Asteraceae have been identified, confirmed as distinct or segregated as new species. During August of 2022, a downy mildew was observed on potted Echinacea purpurea grown in a retail greenhouse in Jefferson County, Wisconsin, USA. Phylogenetic analyses of the cytochrome c oxidase subunit 2 ( cox2 ) and nuclear large subunit ribosomal RNA (nc LSU rDNA) gene regions indicated these Plasmopara sp. isolates are not conspecific with P. halstedii . Based on phylogenetic evidence and new host association, the Plasmopara isolates from E. purpurea are here described as Plasmopara echinaceae . Diagnostic morphological characters for this new species were not observed when compared with other isolates of P. halstedii or other Plasmopara species found on Asteraceae hosts, and therefore a list of species-specific substitutions in the cox2 region are provided as diagnostic characters. As this study corresponds to the first observation of downy mildew in cone flowers, it is recommended to follow the required disease prevention guidelines to prevent outbreaks and the establishment of this plant pathogen in production sites.","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135649800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号