Ukrainskii biokhimicheskii zhurnal (1978)最新文献

The mechanisms of TCR/CD3 complex and phospholipase C interaction, inositol-1,4,5-triphosphate role in both initial transient and sustained elevation of intracellular Ca2+ and in calcium oscillations driving following T cells stimulation are discussed. Data on IP3-receptors structure and localisation in lymphocytes are summarized.

The influence of the tricyclic antidepressants imipramine and ftoracizin on platelet aggregation and smooth muscle contractility was investigated in comparison with action of known smooth muscle relaxant and platelet aggregation inhibitor, papaverine. It has been shown that the tricyclic antidepressants possess potent spasmolytic activity but unlike papaverine have no effect on platelet aggregation. The biochemical mechanisms of the non-specific action of tricyclic antidepressants as well as some other structurally related-drugs are discussed.

The structure, physical, chemical and catalitical properties, functions and biological role of mammalian basic carboxypeptidases are observing. On the strength of the genetic and filogenic research data it is supposed the existence of a family of basic metal-dependent carboxypeptidases and the plan of it evolution is proposed. The connections between structure, localisation and function of this enzyme are discussed.

Analysis of 17 haemostasis indexes has been done in 10 insulin-dependent diabetes mellitus patients, 5 insulin-independent diabetes mellitus patients and in 9 healthy donors. It was determined some of the same type changes and peculiarities which must be taken into consideration both by the estimation of blood system coagulation activity of patients treatment and by definition of disease prognosis etc.

Genome instability of adaptive nature is formed under the experimental influence on a cell. Under critical conditions, strategy of organism is to damage the cells that cannot be restored and controlled by including the program of apoptosis. The ordered internucleosomal DNA degradation is considered to be one of the proof attributes of immunocompetent cell apoptosis. We investigated the effects of various doses of irradiation on the thymocytes chromatine fragmentation in 1,2,3 hours after a single X-ray exposure or after chronic influence in conditions of Chernobyl research base. By the means of electrophoresis in agarose and judging by polydeoxyribonucleotides accumulation we observed the "ladder pattern" of degradation in 3 hr after single 1 Gr irradiation (the smallest dose displaying the effect). We suppose that the influence of both chronic low-intensity irradiation taking place in Chernobyl and single X-ray exposure result in intensifying of DNA fragmentation in the cells of immunocompetent organs.

The alimentary tocopherol deficiency is accompanied by decreased hydroxylase, demethylase, NADH- and NADPH-reductase, aldehyde dehydrogenase, arylesterase and glutathione reductase activity in rat's liver. It decreased the reduced glutathione and increased it's oxidized form concentration in the tocopherol deficient animals. The stability of microsomal membrane is decreased to solubilizing action of deoxycholate and trypsin. This changes, possibly, caused elevation of alteration of function enzyme's and microsomal membrane after nitrosodimethylamine (NDMA) administration in deficient rats. The 7-days injection of tocopherol (20 and 100 mg/kg), dibunol (80 mg/kg), sodium selenite (30 mkg/kg) increased aldehyde dehydrogenase, esterase, glutathione-dependent enzymes activity and increased of reduced glutathione concentration in liver, suppressed lipid peroxidation and increased survival rats after lethal dose carcinogen treatment. Supplementation of tocopherol decreased harmful action of nitrosodimethylamine on microsomal membrane and enzymes activity.

The organization of cytochrome systems in pelagic (Mugil so-iuy) and benthic (Platichthys flesus luscus) species of sea fishes were investigated. The total cytochrome content was 3.6-3.7 times higher (p < 0.001) in muscles of active M. so-iuy and made 67.5 +/- 5.3 nM g-1 for red and 5.91 +/- 0.58 nM g-1 for white muscle tissue. Cytochrome stechiometry had non-compensation character in benthic P. flesus luscus. Cytochrome ratio-b/aa3, was less than one and was equivalent 0.80-0.83 for both muscle groups. On the contrary it was higher than one--1.47-1.66 (p < 0.001) in M. so-iuy. Differences in cytochrome organization were connected with oxygen state of muscle tissue of these fishes. Mean oxygen tension in P. flesus luscus muscles was lower for 20.1-22.5% (p < 0.001). A number of anoxic and hypoxic zones (less than 6 mm Hg) in muscle tissue of this fish made 70 and 4-6% for white and red muscles accordingly. This value was 1.7-2.5 times lower in M. so-iuy muscles.

This article shows that in oxygen transported and antioxidative systems considerable changes take place during dynamic of adaptation to oxygen deficiency of inhaled air (every day 4 hours stay of animals in barochamber under pO2 7.45 kPa during 12 days). Single influence of hypoxic factor induced in erythrocytes peripheral blood increasing of haemoglobin affinity to oxygen, decreasing SOD and catalase activity on the background of increasing of malon aldehyde level. On 12-th day of adaptation of animals contents of TBA-active products in erythrocytes is saved on high level, activity of antioxidative enzymes is approached to original meanings, haemoglobin affinity to oxygen is decreased in comparison with control. Detected increasing content of creatine in erythrocytes during dynamic of rat adaptation to hypoxic hypoxia witnessed about erythropoiesis activation and quickened exit out of marrow young forms of erythrocytes.

Pigs intoxication by chloride manganese in dose 50 mg/kg perk day for 10 days brings to lowering antioxidant protection in blood and the level of respiratory neutrophils' burst. In liver and kidneys superoxide dismutase activity increases, in liver--increasing of initiate and final malondialdehyde contents and lowering of concentration of restored glutathione and ATP.

The comparative study of the sensitivity of Na+, K(+)-ATPase isozymes from cerebral cortex to ascorbate-dependent membrane peroxidation was conducted. With highly inactivated Na+, K(+)-ATPase the degree of inactivation of the SH-dependent ouabain-sensitive forms alpha+ (alpha 2 and alpha 3) is higher than glycoside-resistant isoform alpha 1. The process is accompanied by simultaneous lipid peroxidation and decrease of SH-groups amount in enzyme preparations. The combined nature of the oxidative Na+, K(+)-ATPase inactivation, accompanied by the direct oxidation of enzyme SH-groups and modification of lipid environment is supposed.