Viral outbreaks are the leading causes of economic losses in the poultry industries worldwide. Intensive vaccination programs and excellent management are crucial for successful protection. Certainly, the most vital management factors are feed and water quality. This study evaluated the effect of mycotoxins fed or hard water on IBD, ND, and IB vaccines response and performance in broilers. A total of 400 Cobb chicks were randomly divided into 4 groups; not vaccinated negative control (G1), vaccinated positive control (G2), vaccinated mycotoxins fed (G3) and vaccinated hard water (G4). Blood samples were collected at 4, 14, 21, 28, 35 and 42 days of age. Profolk Plus IBD ELISA Kit, Biocheck IB ELISA Kit and Hemagglutination inhibition (HI) test were used for titers estimation. The feed intake, body weight and the feed conversion ratio were calculated. For the effect of mycotoxins, the G3 showed significantly lower mean titers than G2 from the 35th day for all tested vaccines. For the effect of hard water on IBD vaccine response, the G4 had significantly lower mean titers than G2 on the 42nd day. For IB vaccine response, from the 28th day G4 had significantly lower mean titers than G2. Regarding feed intake and body weight, the G2 was significantly better than G3 and G4. In conclusion, mycotoxins had adverse effects on the humoral immune response for IBD, ND, and IB titers. The adverse effect of hard water on IB vaccines response was significant from the 28th day.
病毒爆发是全世界家禽业经济损失的主要原因。强化疫苗接种规划和优秀的管理是成功保护的关键。当然，最重要的管理因素是饲料和水质。本研究评估了饲喂真菌毒素或硬水对肉鸡IBD、ND和IB疫苗应答和生产性能的影响。选取400只科布雏鸡，随机分为4组;未接种疫苗的阴性对照(G1)、接种疫苗的阳性对照(G2)、接种真菌毒素饲料(G3)和接种硬水(G4)。分别于4、14、21、28、35、42日龄采血。采用Profolk Plus IBD酶联免疫吸附测定试剂盒、Biocheck IB酶联免疫吸附测定试剂盒和血凝抑制(HI)试验进行效价测定。计算采食量、体重和饲料系数。对于霉菌毒素的影响，从第35天开始，所有测试疫苗的G3的平均滴度明显低于G2。对于硬水对IBD疫苗应答的影响，G4在第42天的平均滴度显著低于G2。对于IB疫苗应答，从第28天开始，G4的平均滴度显著低于G2。采食量和体重方面，G2显著优于G3和G4。总之，真菌毒素对IBD、ND和IB滴度的体液免疫反应有不利影响。从第28天开始，硬水对IB疫苗应答的不利影响显著。
{"title":"Effect of Mycotoxins or Hard Water on Newcastle, Gumboro, and Infectious Bronchitis Post Vaccinal Immune Responses and Performance of Broilers","authors":"Ramy Alazzouny, E. Badawy, M. Badawy, T. Ismail","doi":"10.5455/ajvs.158294","DOIUrl":"https://doi.org/10.5455/ajvs.158294","url":null,"abstract":"Viral outbreaks are the leading causes of economic losses in the poultry industries worldwide. Intensive vaccination programs and excellent management are crucial for successful protection. Certainly, the most vital management factors are feed and water quality. This study evaluated the effect of mycotoxins fed or hard water on IBD, ND, and IB vaccines response and performance in broilers. A total of 400 Cobb chicks were randomly divided into 4 groups; not vaccinated negative control (G1), vaccinated positive control (G2), vaccinated mycotoxins fed (G3) and vaccinated hard water (G4). Blood samples were collected at 4, 14, 21, 28, 35 and 42 days of age. Profolk Plus IBD ELISA Kit, Biocheck IB ELISA Kit and Hemagglutination inhibition (HI) test were used for titers estimation. The feed intake, body weight and the feed conversion ratio were calculated. For the effect of mycotoxins, the G3 showed significantly lower mean titers than G2 from the 35th day for all tested vaccines. For the effect of hard water on IBD vaccine response, the G4 had significantly lower mean titers than G2 on the 42nd day. For IB vaccine response, from the 28th day G4 had significantly lower mean titers than G2. Regarding feed intake and body weight, the G2 was significantly better than G3 and G4. In conclusion, mycotoxins had adverse effects on the humoral immune response for IBD, ND, and IB titers. The adverse effect of hard water on IB vaccines response was significant from the 28th day.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84707000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zakaria Bayoumi, A. Edris, Hossam Hossam, M. Latif, R. Shawish
Physico-chemical and microbiological quality of meat products are the main acceptability issue to the consumers. So, the current study aimed to determine the acceptability and fitness of four restructured meat products for human consumption based on their sensory, keeping quality and bacteriological quality trough examination of one-hundred and sixty random samples of minced meat, sausage, beef burger and frankfurter (40 of each), which were collected from different supermarkets located in Menoufia governorate, Egypt, and were examined according to standard guidelines. Results revealed that the examined burger and frankfurter samples revealed higher acceptability scores than those of minced meat and sausage samples. In addition, although it revealed lower pH values, minced meat samples had the lowest keeping quality with significant higher TVN and TBA values than the recorded results of the other examined samples. Moreover, the bacteriological examinations revealed detection of Salmonella and E. coli were detected in 20% and 25%; 17.5% and 20%; 12.5% and 12.5%; 5.0% and 7.5% for the examined minced meat, sausage, burger and frankfurter samples, respectively. In addition, S. aureus was counted (CFU/g) in the examined samples with mean values of 1.98×103, 9.64×102, 5.03×102 and 2.16×102 CFU/g for the same examined samples, respectively. Furthermore, minced meat was the most contaminated samples with Pseudomonas and Aeromonas spp., where P. fluorescens and A. sorbia were the most isolated strains in the examined samples. Meat and meat product safety and acceptability are of a major concern as commercial foods. Minced meat showed lower acceptability levels than the other examined samples, with higher contamination levels. So, monitoring of the chemical and bacteriological quality of restructured meat products is significantly helping in its improvement.
{"title":"Fitness of Some Meat Products for Human Consumption in Relation to Their Physico-Chemical and Bacteriological Quality in the Egyptian Market","authors":"Zakaria Bayoumi, A. Edris, Hossam Hossam, M. Latif, R. Shawish","doi":"10.5455/ajvs.144651","DOIUrl":"https://doi.org/10.5455/ajvs.144651","url":null,"abstract":"Physico-chemical and microbiological quality of meat products are the main acceptability issue to the consumers. So, the current study aimed to determine the acceptability and fitness of four restructured meat products for human consumption based on their sensory, keeping quality and bacteriological quality trough examination of one-hundred and sixty random samples of minced meat, sausage, beef burger and frankfurter (40 of each), which were collected from different supermarkets located in Menoufia governorate, Egypt, and were examined according to standard guidelines. Results revealed that the examined burger and frankfurter samples revealed higher acceptability scores than those of minced meat and sausage samples. In addition, although it revealed lower pH values, minced meat samples had the lowest keeping quality with significant higher TVN and TBA values than the recorded results of the other examined samples. Moreover, the bacteriological examinations revealed detection of Salmonella and E. coli were detected in 20% and 25%; 17.5% and 20%; 12.5% and 12.5%; 5.0% and 7.5% for the examined minced meat, sausage, burger and frankfurter samples, respectively. In addition, S. aureus was counted (CFU/g) in the examined samples with mean values of 1.98×103, 9.64×102, 5.03×102 and 2.16×102 CFU/g for the same examined samples, respectively. Furthermore, minced meat was the most contaminated samples with Pseudomonas and Aeromonas spp., where P. fluorescens and A. sorbia were the most isolated strains in the examined samples. Meat and meat product safety and acceptability are of a major concern as commercial foods. Minced meat showed lower acceptability levels than the other examined samples, with higher contamination levels. So, monitoring of the chemical and bacteriological quality of restructured meat products is significantly helping in its improvement.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90175252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Bolaji, O. Adekunle, Teslim Adebayo, A. Ajayi, Busayomi Dada
ABSTRACT Incidence of intestinal parasites has been described as one of the most common problems of developing countries like Nigeria. This study was carried out to determine the possible presence of parasites amongst asymptomatic food handlers in tertiary institution in Owo. One hundred and twenty three (123) stool samples were randomly collected from food vendors in Achievers University, Owo. Also, questionnaires were administered and collated. The samples were transported to the University Parasitology Laboratory within 1 hour of production and examined microscopically using physiological saline and iodine direct mount and formal ether concentration techniques. Out of the 123 stool samples analyzed, 20 were infected with gastrointestinal parasitic organisms, giving a prevalence of 16.26%. Three different parasites were identified: Ascaris lumbricoides (12.20%), Giardia lambia (5.67%), and Entamoeba histolytica (3.25%). The prevalence with respect to gender showed that parasitic infestation was higher in females (9.76%) than in males (6.50%). The parasitic infestation was higher in age groups 36–45 years (7.32%), followed by age groups 18–25 years (5.69) and least in age group 26–35 years (3.25%). The parasitic infestation was higher among subjects who sell cooked food and snacks (6.50%), followed by those who sell cooked food only (4.88%), and lastly 2.44% among those that sell snacks and roasted meat respectively. In conclusion, this study confirmed the presence of intestinal parasitic infection among food handlers in Achievers University Owo, Ondo State. Good hygiene and safe food-handling should be exercised by food handlers.
{"title":"Intestinal Parasites among Asymptomatic Food Handlers in a Tertiary Institution in Owo, Ondo State, Nigeria","authors":"O. Bolaji, O. Adekunle, Teslim Adebayo, A. Ajayi, Busayomi Dada","doi":"10.5455/ajvs.120293","DOIUrl":"https://doi.org/10.5455/ajvs.120293","url":null,"abstract":"ABSTRACT Incidence of intestinal parasites has been described as one of the most common problems of developing countries like Nigeria. This study was carried out to determine the possible presence of parasites amongst asymptomatic food handlers in tertiary institution in Owo. One hundred and twenty three (123) stool samples were randomly collected from food vendors in Achievers University, Owo. Also, questionnaires were administered and collated. The samples were transported to the University Parasitology Laboratory within 1 hour of production and examined microscopically using physiological saline and iodine direct mount and formal ether concentration techniques. Out of the 123 stool samples analyzed, 20 were infected with gastrointestinal parasitic organisms, giving a prevalence of 16.26%. Three different parasites were identified: Ascaris lumbricoides (12.20%), Giardia lambia (5.67%), and Entamoeba histolytica (3.25%). The prevalence with respect to gender showed that parasitic infestation was higher in females (9.76%) than in males (6.50%). The parasitic infestation was higher in age groups 36–45 years (7.32%), followed by age groups 18–25 years (5.69) and least in age group 26–35 years (3.25%). The parasitic infestation was higher among subjects who sell cooked food and snacks (6.50%), followed by those who sell cooked food only (4.88%), and lastly 2.44% among those that sell snacks and roasted meat respectively. In conclusion, this study confirmed the presence of intestinal parasitic infection among food handlers in Achievers University Owo, Ondo State. Good hygiene and safe food-handling should be exercised by food handlers.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89320468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hoda AbdAllah, Abd Hamed, Mohamed Bakry, Ibtisam Eldin, Magda AbdElal, Dalia Mohamed, Sahar Mohamady
Climatic change is a grave and growing threat to natural systems and aquatic species. Cold stress has caused a great worldwide economic loss in aquacultures worldwide. This study aimed to evaluate the effect of dietary Spirulina (Arthrospira platensis) on immunobiochemical parameters, antioxidants, some metabolic gene expressions, and histological structure of liver and kidney of Nile tilapia (Oreochromis niloticus) under cold stress. One hundred and sixty Nile tilapia were divided into eight groups (10 fish per aquarium) in two replicates. The first four groups were maintained at 26 °C whereas, the rest were subjected to 17 °C cold stress. They were fed three spirulina-contained diets (0.75, 1.5, and 3%) and a control diet for three weeks. Blood and tissue samples were collected from all groups after exposure to cold stress. The results of the cold-stressed group that fed control diet showed a significant decreases in the serum level of cortisol, catalase, superoxide dismutase, phagocytic activity, tumor necrotic factor α, Interleukin 1β, Interleukin 6, and Interleukin 17. Also, it explored a significant increased serum level of heat shock protein 70, glucose, Interleukin 10, and malondialdehyde, beside up-regulation of Phosphoenolpyruvate carboxykinase 1, and mitochondrial transcription factor A genes. In addition to, alteration of liver and kidney biomarkers with severe histopathological perturbations of liver and kidney were detected. Dietary Spirulina supplementation at 0.75% during cold stress ameliorate the immune and antioxidant status, biochemical parameters and protecting the integrated tissue architecture from oxidative damage of Nile tilapia.
{"title":"Impact of Spirulina Supplementation on Some Immune and Biochemical Parameters of Nile Tilapia Under Cold Stress","authors":"Hoda AbdAllah, Abd Hamed, Mohamed Bakry, Ibtisam Eldin, Magda AbdElal, Dalia Mohamed, Sahar Mohamady","doi":"10.5455/ajvs.164820","DOIUrl":"https://doi.org/10.5455/ajvs.164820","url":null,"abstract":"Climatic change is a grave and growing threat to natural systems and aquatic species. Cold stress has caused a great worldwide economic loss in aquacultures worldwide. This study aimed to evaluate the effect of dietary Spirulina (Arthrospira platensis) on immunobiochemical parameters, antioxidants, some metabolic gene expressions, and histological structure of liver and kidney of Nile tilapia (Oreochromis niloticus) under cold stress. One hundred and sixty Nile tilapia were divided into eight groups (10 fish per aquarium) in two replicates. The first four groups were maintained at 26 °C whereas, the rest were subjected to 17 °C cold stress. They were fed three spirulina-contained diets (0.75, 1.5, and 3%) and a control diet for three weeks. Blood and tissue samples were collected from all groups after exposure to cold stress. The results of the cold-stressed group that fed control diet showed a significant decreases in the serum level of cortisol, catalase, superoxide dismutase, phagocytic activity, tumor necrotic factor α, Interleukin 1β, Interleukin 6, and Interleukin 17. Also, it explored a significant increased serum level of heat shock protein 70, glucose, Interleukin 10, and malondialdehyde, beside up-regulation of Phosphoenolpyruvate carboxykinase 1, and mitochondrial transcription factor A genes. In addition to, alteration of liver and kidney biomarkers with severe histopathological perturbations of liver and kidney were detected. Dietary Spirulina supplementation at 0.75% during cold stress ameliorate the immune and antioxidant status, biochemical parameters and protecting the integrated tissue architecture from oxidative damage of Nile tilapia.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahmoud Sedeik, Nahed Shall, Ahmed Salaheldin, Raghda Goda, Ashraf Awad
The antiviral and immunostimulant effect of dietary supplementation of Spirulina platensis (SP) against Infectious bursal disease (IBD) was assessed as follows; 126 one-day-old cobb broiler chicks were randomly distributed into six groups (21 birds/group with three replicates, 7 birds each): negative control (non-vaccinated, and non-challenged ; NC), positive control (non-vaccinated, and challenged; PC), vaccinated control (vaccinated, and challenged; VC), Spirulina 0.1% (vaccinated, 0.1% SP-supplemented, and challenged; VSP-0.1), Spirulina 0.3% (vaccinated, 0.3% SP-supplemented, and challenged; VSP-0.3), and Spirulina 0.5% (vaccinated, 0.5% SP-supplemented, and challenged; VSP-0.5). The chicks in NC, PC, and VC groups were not supplemented with SP. All vaccinated groups, regardless SP supplementation, showed significant improvement of body weight gain (19% in 0.3% SP) and FCR during IBDv challenge (10 dpc) (P˂0.05). On the 10th dpc, IBD ELISA titer in SP-supplemented groups were decreased (P>0.05) than that of G3, suggesting an antiviral efficacy of SP. This antiviral activity was evident by the viral RNA load in the cloacal swabs as well. The viral shedding peak was on the 5th day pc which was significantly decreased on G4-6 (P˂0.05) in comparison to G2 and G3. Also, the bursal ratio was improved in SP supplemented groups comparable to G3. Moreover, in unchallenged broilers, the gene expression of INF-γ and IL-18 were upregulated while that of IL-10 was downregulated in spleen of SP-supplemented chicks. Finally, severe histopathological lesions of kidneys, bursa, thymus, and spleen were observed in G2 which moderately minimized in G3 and were more alleviated in SP-supplemented groups. Collectively, SP supplementation improved body weight gain and decreasing viral shedding with antiviral activity. The mechanisms of SP against IBDV challenge may include cell-mediated immunity promoting effect, antiviral activity, and/or anti-inflammatory effect that should be further studied
{"title":"Evaluation of Spirulina Platensis Efficacy Against Local Field Vvibdv Challenge in Broiler Chickens","authors":"Mahmoud Sedeik, Nahed Shall, Ahmed Salaheldin, Raghda Goda, Ashraf Awad","doi":"10.5455/ajvs.148911","DOIUrl":"https://doi.org/10.5455/ajvs.148911","url":null,"abstract":"The antiviral and immunostimulant effect of dietary supplementation of Spirulina platensis (SP) against Infectious bursal disease (IBD) was assessed as follows; 126 one-day-old cobb broiler chicks were randomly distributed into six groups (21 birds/group with three replicates, 7 birds each): negative control (non-vaccinated, and non-challenged ; NC), positive control (non-vaccinated, and challenged; PC), vaccinated control (vaccinated, and challenged; VC), Spirulina 0.1% (vaccinated, 0.1% SP-supplemented, and challenged; VSP-0.1), Spirulina 0.3% (vaccinated, 0.3% SP-supplemented, and challenged; VSP-0.3), and Spirulina 0.5% (vaccinated, 0.5% SP-supplemented, and challenged; VSP-0.5). The chicks in NC, PC, and VC groups were not supplemented with SP. All vaccinated groups, regardless SP supplementation, showed significant improvement of body weight gain (19% in 0.3% SP) and FCR during IBDv challenge (10 dpc) (P˂0.05). On the 10th dpc, IBD ELISA titer in SP-supplemented groups were decreased (P>0.05) than that of G3, suggesting an antiviral efficacy of SP. This antiviral activity was evident by the viral RNA load in the cloacal swabs as well. The viral shedding peak was on the 5th day pc which was significantly decreased on G4-6 (P˂0.05) in comparison to G2 and G3. Also, the bursal ratio was improved in SP supplemented groups comparable to G3. Moreover, in unchallenged broilers, the gene expression of INF-γ and IL-18 were upregulated while that of IL-10 was downregulated in spleen of SP-supplemented chicks. Finally, severe histopathological lesions of kidneys, bursa, thymus, and spleen were observed in G2 which moderately minimized in G3 and were more alleviated in SP-supplemented groups. Collectively, SP supplementation improved body weight gain and decreasing viral shedding with antiviral activity. The mechanisms of SP against IBDV challenge may include cell-mediated immunity promoting effect, antiviral activity, and/or anti-inflammatory effect that should be further studied","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135447648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Omotosho, Sandra Ukatu, M. Okandeji, O. Okunlade, A. Adeola, B. Emikpe
There is increasing global threat of antimicrobial resistance (AMR) mostly with regards to the exchange of potentially harmful microbes across human and animal populations. There is also limited information in literature on the microbial population of Nigerian Indigenous Pigs (NIP). This study was designed as a preliminary investigation to provide baseline information on the AMR profile of nasal flora of NIP. Thirty-one nasal swabs were collected from NIPs on the Teaching and Research Farm, University of Ibadan, Nigeria. Bacteria were isolated, characterized and identified using cultural and biochemical characteristics. Antibiotic Susceptibility Testing (AST) was carried out on the pure strains of bacteria using the gram positive and negative antibiotic sensitivity discs. Three media were used for isolation; Nutrient agar, MacConkey agar and Mannitol Salt Agar. Data were analysed using descriptive statistics. Ten multi-drug resistant bacteria were identified; Staphylococcus aureus (96.8%), Escherichia coli (87.1%), Shigella species (80.6%), Bacillus species 51.6% (16/31) and Serratia species 51.6% (16/31) were the most frequently isolated. Of the isolated organisms, the gram positive organisms showed high resistance to Ceftazidime (81.1%), Ceftriaxone (86.9%), Cloxacilin (93.4%), Augmentin (100%), and Cefuroxime (100%) with considerable sensitivity to Ofloxacin (74.6%) and Gentamicin (80.9%). While the gram negative bacteria were highly resistant to Tetracycline (77.6%), Cefuroxime (90%), Ceftriaxone (87.1%), Sulbactarm (83.2%) and Ampiclox (85.7%) with considerable sensitivity to Levofloxacin (78.9%) and Nitrofurantoin (93.4%). This study reveals a high microbial load of AMR bacteria in the nasal cavity of NIPs making them a possible reservoir. These findings unveil the need for genetic data to determine the pattern, extent and basis of AMR resistance in the nasal flora of NIPs. We therefore recommend more rational use of antibiotics in pigs and other food animals to safeguard animal and human health.
{"title":"Antimicrobial Resistance Profile of Aerobic Nasal Microflora of Nigerian Indigenous Pigs","authors":"O. Omotosho, Sandra Ukatu, M. Okandeji, O. Okunlade, A. Adeola, B. Emikpe","doi":"10.5455/ajvs.70219","DOIUrl":"https://doi.org/10.5455/ajvs.70219","url":null,"abstract":"There is increasing global threat of antimicrobial resistance (AMR) mostly with regards to the exchange of potentially harmful microbes across human and animal populations. There is also limited information in literature on the microbial population of Nigerian Indigenous Pigs (NIP). This study was designed as a preliminary investigation to provide baseline information on the AMR profile of nasal flora of NIP. Thirty-one nasal swabs were collected from NIPs on the Teaching and Research Farm, University of Ibadan, Nigeria. Bacteria were isolated, characterized and identified using cultural and biochemical characteristics. Antibiotic Susceptibility Testing (AST) was carried out on the pure strains of bacteria using the gram positive and negative antibiotic sensitivity discs. Three media were used for isolation; Nutrient agar, MacConkey agar and Mannitol Salt Agar. Data were analysed using descriptive statistics. Ten multi-drug resistant bacteria were identified; Staphylococcus aureus (96.8%), Escherichia coli (87.1%), Shigella species (80.6%), Bacillus species 51.6% (16/31) and Serratia species 51.6% (16/31) were the most frequently isolated. Of the isolated organisms, the gram positive organisms showed high resistance to Ceftazidime (81.1%), Ceftriaxone (86.9%), Cloxacilin (93.4%), Augmentin (100%), and Cefuroxime (100%) with considerable sensitivity to Ofloxacin (74.6%) and Gentamicin (80.9%). While the gram negative bacteria were highly resistant to Tetracycline (77.6%), Cefuroxime (90%), Ceftriaxone (87.1%), Sulbactarm (83.2%) and Ampiclox (85.7%) with considerable sensitivity to Levofloxacin (78.9%) and Nitrofurantoin (93.4%). This study reveals a high microbial load of AMR bacteria in the nasal cavity of NIPs making them a possible reservoir. These findings unveil the need for genetic data to determine the pattern, extent and basis of AMR resistance in the nasal flora of NIPs. We therefore recommend more rational use of antibiotics in pigs and other food animals to safeguard animal and human health.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91248813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ola Elkhateeb, M. Badawy, H. Ahmed, M. Kammar, H. Elkhenany
Natural extracts (EXTR) have been proven to effectively regenerate tissues however may need long treatment periods. Here in this study, we investigated curcumin (CURC) and Propolis (PROP) encapsulated in the form of nanostructure lipid carrier (NLC) form. The results have shown that this technology has preserved a higher flavonoid content compared to crude EXTR from both materials under investigation. Interestingly, phenolic content in PROP NLC was significantly higher than EXTR, however, CURC NLC did not show the same pattern compared to its relative EXTR. Further investigations are needed to prove the efficacy of this PROP and CURC NLC as antioxidant, antibacterial and wound healing proficiency.
{"title":"Nanostructured Lipid Carrier Technology to Enhance Natural Extracts Delivery: Potential of Skin Regeneration","authors":"Ola Elkhateeb, M. Badawy, H. Ahmed, M. Kammar, H. Elkhenany","doi":"10.5455/ajvs.107122","DOIUrl":"https://doi.org/10.5455/ajvs.107122","url":null,"abstract":"Natural extracts (EXTR) have been proven to effectively regenerate tissues however may need long treatment periods. Here in this study, we investigated curcumin (CURC) and Propolis (PROP) encapsulated in the form of nanostructure lipid carrier (NLC) form. The results have shown that this technology has preserved a higher flavonoid content compared to crude EXTR from both materials under investigation. Interestingly, phenolic content in PROP NLC was significantly higher than EXTR, however, CURC NLC did not show the same pattern compared to its relative EXTR. Further investigations are needed to prove the efficacy of this PROP and CURC NLC as antioxidant, antibacterial and wound healing proficiency.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84398439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Sule, M. Omokanye, O. Adekunle, Jemilat Abdulkareem, I. Adebesin, Olawuyi Awofisayo, Shamah A. Agboola, Wasilat Abdulwaheed
Cattle can harbour enterohaemorrhagic Escherichia coli O157:H7 serotype in their faecal matters. This study aimed to isolate E. coli O157:H7 from the intestinal digesta, cattle dungs and water; and assess the antibiotic susceptibility and production of shiga toxins by the isolates. The counts of viable bacteria and faecal coliforms in 13 each of intestinal digesta and cattle dung and the 12 water samples were determined using nutrient agar and Eosin methylene blue agar respectively. Sorbitol MacConkey agar (SMAC) was used to screen for E. coli O157:H7 among the 38 E. coli isolates. PCR amplification of uidA genes was used to authenticate the isolates as enterohaemorrhagic E. coli O157:H7 serotype while amplification of stx1 and stx2 showed the production of shiga toxins. The antibiotic susceptibility patterns of the isolates were determined using standard disk diffusion method. The count of viable bacteria and faecal coliform was highest in the intestinal digesta followed by cattle dungs. There was 100% susceptibility to ofloxacin coupled with 100% resistant to augmentin by all the 8 E. coli O157 and 10 non-O157 isolates. The E. coli O157 isolates were more susceptible to ciprofloxacin, gentamicin, ampicillin and cefuroxime than non-O157 isolates but were less susceptible to nitrofurantoin and ceftazidime than non-O157. Eight (44.4%) out of the 18 presumptive E. coli O157 on SMAC amplified uidA genes and were confirmed as E. coli O157. They were isolated only from intestinal digesta and cattle dung. The prevalence of stx1 and stx2 genes among the E. coli O157 was 37.5% and 12.5% respectively. It is concluded from this study that intestinal digesta and cattle dung harboured E. coli O157: H7 some of which possessed shiga toxin.
{"title":"Detection of uidA, stx1, and stx2 Genes in Escherichia coli O157:H7 Isolated from Cattle Faecal Matter and River Water","authors":"I. Sule, M. Omokanye, O. Adekunle, Jemilat Abdulkareem, I. Adebesin, Olawuyi Awofisayo, Shamah A. Agboola, Wasilat Abdulwaheed","doi":"10.5455/ajvs.136270","DOIUrl":"https://doi.org/10.5455/ajvs.136270","url":null,"abstract":"Cattle can harbour enterohaemorrhagic Escherichia coli O157:H7 serotype in their faecal matters. This study aimed to isolate E. coli O157:H7 from the intestinal digesta, cattle dungs and water; and assess the antibiotic susceptibility and production of shiga toxins by the isolates. The counts of viable bacteria and faecal coliforms in 13 each of intestinal digesta and cattle dung and the 12 water samples were determined using nutrient agar and Eosin methylene blue agar respectively. Sorbitol MacConkey agar (SMAC) was used to screen for E. coli O157:H7 among the 38 E. coli isolates. PCR amplification of uidA genes was used to authenticate the isolates as enterohaemorrhagic E. coli O157:H7 serotype while amplification of stx1 and stx2 showed the production of shiga toxins. The antibiotic susceptibility patterns of the isolates were determined using standard disk diffusion method. The count of viable bacteria and faecal coliform was highest in the intestinal digesta followed by cattle dungs. There was 100% susceptibility to ofloxacin coupled with 100% resistant to augmentin by all the 8 E. coli O157 and 10 non-O157 isolates. The E. coli O157 isolates were more susceptible to ciprofloxacin, gentamicin, ampicillin and cefuroxime than non-O157 isolates but were less susceptible to nitrofurantoin and ceftazidime than non-O157. Eight (44.4%) out of the 18 presumptive E. coli O157 on SMAC amplified uidA genes and were confirmed as E. coli O157. They were isolated only from intestinal digesta and cattle dung. The prevalence of stx1 and stx2 genes among the E. coli O157 was 37.5% and 12.5% respectively. It is concluded from this study that intestinal digesta and cattle dung harboured E. coli O157: H7 some of which possessed shiga toxin.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73137043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vaccines are the most effective method for animal immunization against infectious diseases. This work aimed to study the effect of some vaccines commonly, used in sheep, on some innate immunological and clinicopathological parameters. For this purpose, forty-five Barki ewes were housed at the sustainable development Centre of Matrouh resources farm. They were divided into: Pox group: fifteen ewes were injected with live-attenuated pox vaccine. FMD+RVF group: fifteen ewes were injected separately with two vaccines (polyvalent inactivated foot and mouth disease oil adjuvant vaccine and rift valley inactivated vaccine). Ultrabac group: fifteen ewes were injected with 2.5 ml bacterial toxoid (Ultrabac® 8, Zoetis). Blood samples were collected then immunological and clinicopathological parameters were estimated and statistically analyzed. The three studied groups showed a significant (P˂0.05) increase in the activity of pro-inflammatory cytokines, ceruloplasmin, and oxidative stress, associated with serious clinicopathological changes (anemia, leukocytosis, increased liver and kidney functions, hyperglobulinemia, hypoalbuminemia). These alterations peaked on the 14th day, and subsequently moved towards their baseline values. They were more prominent and sustained in the Ultrabac group (with selective hyperproteinemia) than in the other two groups. The FMD+RVF group displayed a higher degree of cytokines activity, APR, oxidative stress, leukocytosis, and AST values than the Pox group. Conclusion: Sheep vaccination evokes an innate immune response which results in clinicopathological changes. These alterations are more pronounced in bacterial and combined vaccines than viral and single vaccines. The first 14 days after vaccination is a critical period and needs proper management.
{"title":"Clinicopathological study on some vaccines in Barki ewes","authors":"A. Darwish, Ahmed Sify, T. Allam","doi":"10.5455/ajvs.142770","DOIUrl":"https://doi.org/10.5455/ajvs.142770","url":null,"abstract":"Vaccines are the most effective method for animal immunization against infectious diseases. This work aimed to study the effect of some vaccines commonly, used in sheep, on some innate immunological and clinicopathological parameters. For this purpose, forty-five Barki ewes were housed at the sustainable development Centre of Matrouh resources farm. They were divided into: Pox group: fifteen ewes were injected with live-attenuated pox vaccine. FMD+RVF group: fifteen ewes were injected separately with two vaccines (polyvalent inactivated foot and mouth disease oil adjuvant vaccine and rift valley inactivated vaccine). Ultrabac group: fifteen ewes were injected with 2.5 ml bacterial toxoid (Ultrabac® 8, Zoetis). Blood samples were collected then immunological and clinicopathological parameters were estimated and statistically analyzed. The three studied groups showed a significant (P˂0.05) increase in the activity of pro-inflammatory cytokines, ceruloplasmin, and oxidative stress, associated with serious clinicopathological changes (anemia, leukocytosis, increased liver and kidney functions, hyperglobulinemia, hypoalbuminemia). These alterations peaked on the 14th day, and subsequently moved towards their baseline values. They were more prominent and sustained in the Ultrabac group (with selective hyperproteinemia) than in the other two groups. The FMD+RVF group displayed a higher degree of cytokines activity, APR, oxidative stress, leukocytosis, and AST values than the Pox group. Conclusion: Sheep vaccination evokes an innate immune response which results in clinicopathological changes. These alterations are more pronounced in bacterial and combined vaccines than viral and single vaccines. The first 14 days after vaccination is a critical period and needs proper management.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75331591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Bolaji, O. Adekunle, A. Ajayi, Abolaji T. Adeyemo, A. Ojewuyi, A. Ibrahim, A. Adeyemo, A. Ajayi, Akinwumi Akindele, O. Adeyeba
This study was undertaken to determine the prevalence of intestinal parasites and enteropathogenic bacteria in fecal effluents from abattoir. A total of 162 samples were examined out of which 70 faecal samples were collected from cow, 35 from pig, 35 from goat and 22 from sheep. These samples were processed using standard parasitological techniques ( macroscopy, microscopy; the Formol-ether concentration technique and zinc sulphate floatation technique) and bacteriological (culture, Gram reaction and biochemical characterization). Data was analyzed using Chi-square test. One hundred and eleven (111) samples were found positive with one or more parasites giving an overall prevalence of 68.5%. Pigs recorded the highest prevalence of 77.1% for intestinal parasites, followed by goat and cow with prevalence of 68.6% and 65.7% respectively and the least prevalence was observed in sheep, 63.6%. The prevalence of different parasites encountered include Fasciolopsis buski (8.6%), Hookworm (8.0%), Ascaris suum (7.4%), Balantidium coli (7.4%), Fasciola hepatica (7.4%), Entamoeba histolytica (7.4%), Taenia species (6.2%), Fasciola gigantica (4.3%) and Toxocara species (1.2%). Mixed infections of Balantidium coli+Entamoeba histolytica has the highest frequency of 4(2.5%) followed by Entamoeba histolytica+Hookworm and Toxocara species+Balantidium coli with the frequency of 3(1.9%) each, Ascaris suum+Taenia species, Ascaris suum+Taenia species+Hookworm and Balantidium coli+Fasciola hepatica with a frequency of 2(1.2%) each and Toxocara species+Balantidium coli recorded the lowest frequency of 1(0.6%). Also the prevalence of potentially pathogenic bacteria and yeast cells encountered include Bacillus species (29.6%), Escherichia coli (23.5%), Campylobacter species (17.3%), Pseudomonas aeruginosa (11.1%), Staphylococcus aureus (10.5%), Yeast cells (4.9%) and Staphylococcus species (3.1%). This study shows high degrees of fecal contamination of intestinal parasites and bacterial agents. As a result of this sanitary measures and Government policy should be strictly employed as this will go a long way to help check environmental contamination and reduce potential risks posed by these pathogens.
{"title":"Prevalence of Pathogenic Intestinal Parasites and Enteropathogenic Bacteria in Faecal Samples Obtained from Abattoirs in Ogbomoso, Oyo State, Nigeria","authors":"O. Bolaji, O. Adekunle, A. Ajayi, Abolaji T. Adeyemo, A. Ojewuyi, A. Ibrahim, A. Adeyemo, A. Ajayi, Akinwumi Akindele, O. Adeyeba","doi":"10.5455/ajvs.148234","DOIUrl":"https://doi.org/10.5455/ajvs.148234","url":null,"abstract":"This study was undertaken to determine the prevalence of intestinal parasites and enteropathogenic bacteria in fecal effluents from abattoir. A total of 162 samples were examined out of which 70 faecal samples were collected from cow, 35 from pig, 35 from goat and 22 from sheep. These samples were processed using standard parasitological techniques ( macroscopy, microscopy; the Formol-ether concentration technique and zinc sulphate floatation technique) and bacteriological (culture, Gram reaction and biochemical characterization). Data was analyzed using Chi-square test. One hundred and eleven (111) samples were found positive with one or more parasites giving an overall prevalence of 68.5%. Pigs recorded the highest prevalence of 77.1% for intestinal parasites, followed by goat and cow with prevalence of 68.6% and 65.7% respectively and the least prevalence was observed in sheep, 63.6%. The prevalence of different parasites encountered include Fasciolopsis buski (8.6%), Hookworm (8.0%), Ascaris suum (7.4%), Balantidium coli (7.4%), Fasciola hepatica (7.4%), Entamoeba histolytica (7.4%), Taenia species (6.2%), Fasciola gigantica (4.3%) and Toxocara species (1.2%). Mixed infections of Balantidium coli+Entamoeba histolytica has the highest frequency of 4(2.5%) followed by Entamoeba histolytica+Hookworm and Toxocara species+Balantidium coli with the frequency of 3(1.9%) each, Ascaris suum+Taenia species, Ascaris suum+Taenia species+Hookworm and Balantidium coli+Fasciola hepatica with a frequency of 2(1.2%) each and Toxocara species+Balantidium coli recorded the lowest frequency of 1(0.6%). Also the prevalence of potentially pathogenic bacteria and yeast cells encountered include Bacillus species (29.6%), Escherichia coli (23.5%), Campylobacter species (17.3%), Pseudomonas aeruginosa (11.1%), Staphylococcus aureus (10.5%), Yeast cells (4.9%) and Staphylococcus species (3.1%). This study shows high degrees of fecal contamination of intestinal parasites and bacterial agents. As a result of this sanitary measures and Government policy should be strictly employed as this will go a long way to help check environmental contamination and reduce potential risks posed by these pathogens.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89543429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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