Canned meats are a popular food source that provides protein and the advantage of extended shelf life. However, these products are carbohydrates, fats, vitamins to chemical and microbiological spoilage that can cause changes in their texture, flavor, and odor. Therefore, 60 random samples of locally manufactured canned food were collected from different supermarkets in Alexandria governorate, which were divided into three groups 20 each of canned beef, canned chicken, and canned fish. The obtained results revealed that the mean values of FFA, TVN, and TBA of canned beef, chicken, and fish were 1.17±0.08, 1.07±0.08 & 1.27±0.01; 0.12±0.05, 0.13±0.05 & 0.12±0.04 and 13.4±0.89, 12.75±0.44 & 14.52±0.73, respectively, while the mean of TMA in canned fish was 0.64±0.04 mg/100g. Regarding microbiological quality, the mean values of the aerobic bacterial count were 1.85×104±0.49×104, 1.1×104± 0.47×104, and 7.84×103±2.13×103 Cfu/g in examined canned beef, chicken, and fish products; the mean values of anaerobic bacterial count in the same products were 1.02×104±1.23×104, 0.52×104±0.70×104, and 6.0×103±9.01×103 Cfu/g, respectively. All chemical and bacteriological parameters compared with Egyptian and international standards. Bacillus cereus was detected in 25, 20 and 15% of examined canned beef, chicken, and fish products. On the other hand, Clostridium perfringens couldn’t be detected in any examined canned meat products. Contaminated canned meat products highlighted the need to implement food safety systems such as HACCP during the manufacturing of meat meals.
{"title":"Chemical and Microbiological Spoilage of Canned Meat Products","authors":"Marwa Madian, Mohamed Mousa, Kamal Ayoub","doi":"10.5455/ajvs.157089","DOIUrl":"https://doi.org/10.5455/ajvs.157089","url":null,"abstract":"Canned meats are a popular food source that provides protein and the advantage of extended shelf life. However, these products are carbohydrates, fats, vitamins to chemical and microbiological spoilage that can cause changes in their texture, flavor, and odor. Therefore, 60 random samples of locally manufactured canned food were collected from different supermarkets in Alexandria governorate, which were divided into three groups 20 each of canned beef, canned chicken, and canned fish. The obtained results revealed that the mean values of FFA, TVN, and TBA of canned beef, chicken, and fish were 1.17±0.08, 1.07±0.08 & 1.27±0.01; 0.12±0.05, 0.13±0.05 & 0.12±0.04 and 13.4±0.89, 12.75±0.44 & 14.52±0.73, respectively, while the mean of TMA in canned fish was 0.64±0.04 mg/100g. Regarding microbiological quality, the mean values of the aerobic bacterial count were 1.85×104±0.49×104, 1.1×104± 0.47×104, and 7.84×103±2.13×103 Cfu/g in examined canned beef, chicken, and fish products; the mean values of anaerobic bacterial count in the same products were 1.02×104±1.23×104, 0.52×104±0.70×104, and 6.0×103±9.01×103 Cfu/g, respectively. All chemical and bacteriological parameters compared with Egyptian and international standards. Bacillus cereus was detected in 25, 20 and 15% of examined canned beef, chicken, and fish products. On the other hand, Clostridium perfringens couldn’t be detected in any examined canned meat products. Contaminated canned meat products highlighted the need to implement food safety systems such as HACCP during the manufacturing of meat meals.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"152 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135506723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmed Hadary, Iman Bawab, Kamal Metwaly, Samia Rheem
Semen preservation by cooling or freezing is an essential artificial insemination (AI) step. AI is affected by many factors, such as semen quality. The aim of this study was to evaluate the effect of adding different concentrations of different antioxidants such as selenium, zinc oxide and Vitamin C to the diluted buck semen during cooling in both summer and winter. Eight mature healthy Zaraibi bucks were used in the study. semen was collected twice weekly during summer 2021 (august and September) and winter 2022 (January and February). Semen samples were collected by an electro- ejaculator device. Samples of good quality semen were pooled and diluted by extender and divided according to experimental groups: Group 1 Vitamin C: 50 mg /100 ml, 1oo mg /100 ml, 150 mg /100 ml. Group 2 Zinc oxide: 0.5 mg /100 ml, 1 mg /100 ml, 1.5 mg /100 ml. Group 3 Selenium: 100 μL/100 ml, 200 μL / 100, 300 μL / 100. After cooling by 1 hr. semen parameters were examined as motility, livability, acrosome integrity and cell membrane integrity and at 8 hours from cooling then every 8 hr till 64 hr. Seminal antioxidant activities as SOD and catalase were estimated at 0 hr, 24 hr and 48 hr. The results indicated that selenium 200 μL / 100 ml diluent has a favorable effect on cooled diluted buck semen during summer and winter more than selenium 100 μL and 300 μL. Selenium is better than Zinc oxide and Vitamin C for improving the semen quality as with minimum cost. In conclusion adding selenium as antioxidant to buck semen during cooling preservation with 200 μL concentration per 100 diluents is preferred for buck semen during cooling preservation.
{"title":"The Effect of Adding Antioxidants on Cooled Zarabi Buck Semen During Different Seasons","authors":"Ahmed Hadary, Iman Bawab, Kamal Metwaly, Samia Rheem","doi":"10.5455/ajvs.164073","DOIUrl":"https://doi.org/10.5455/ajvs.164073","url":null,"abstract":"Semen preservation by cooling or freezing is an essential artificial insemination (AI) step. AI is affected by many factors, such as semen quality. The aim of this study was to evaluate the effect of adding different concentrations of different antioxidants such as selenium, zinc oxide and Vitamin C to the diluted buck semen during cooling in both summer and winter. Eight mature healthy Zaraibi bucks were used in the study. semen was collected twice weekly during summer 2021 (august and September) and winter 2022 (January and February). Semen samples were collected by an electro- ejaculator device. Samples of good quality semen were pooled and diluted by extender and divided according to experimental groups: Group 1 Vitamin C: 50 mg /100 ml, 1oo mg /100 ml, 150 mg /100 ml. Group 2 Zinc oxide: 0.5 mg /100 ml, 1 mg /100 ml, 1.5 mg /100 ml. Group 3 Selenium: 100 μL/100 ml, 200 μL / 100, 300 μL / 100. After cooling by 1 hr. semen parameters were examined as motility, livability, acrosome integrity and cell membrane integrity and at 8 hours from cooling then every 8 hr till 64 hr. Seminal antioxidant activities as SOD and catalase were estimated at 0 hr, 24 hr and 48 hr. The results indicated that selenium 200 μL / 100 ml diluent has a favorable effect on cooled diluted buck semen during summer and winter more than selenium 100 μL and 300 μL. Selenium is better than Zinc oxide and Vitamin C for improving the semen quality as with minimum cost. In conclusion adding selenium as antioxidant to buck semen during cooling preservation with 200 μL concentration per 100 diluents is preferred for buck semen during cooling preservation.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135506724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Medicinal herbs are the most utilized and validated feed additives in the aquaculture industry. This study investigated the dietary effects of anise (Pimpinella anisum L.) in Nile Tilapia (Oreochromis niloticus). Three sets of diets were prepared by spraying Anise essential oil on a commercial basal diet (30% crude protein) at the rate of 0 (control), 1, and 2 mL/kg diet. The experimental groups were assigned a 3 × 2 factorial design (3 level of anise oil; 0, 1, 2 ml) × 2 levels of dissolved oxygen (DO) concentration. The experiment was assembled as a control no anise (high DO), 1 ml + (high DO), 2 ml + (high DO), Control 0 ml (low DO), 1 ml anise (low DO), and 2 ml anise (low DO). Fish were fed the diets for 60 days consecutively at 3% body weight. The feed intake was recorded daily by subtracting the remaining feed in the containers from the total amount of feed before feeding. The results showed a marked enhancement in the growth performance and reduced feed conversion ratio in fish-fed anise compared to the control group. The carcass composition showed that crude protein was meaningfully by 2.5 and 3.5 g anise per kg diet. In conclusion, incorporating anise into the diet of Nile Tilapia is suggested to enhance performances and well-being.
草药是水产养殖业中最常用和最有效的饲料添加剂。研究了大茴香对尼罗罗非鱼(Oreochromis niloticus)的饲粮效应。将茴香精油分别以0(对照)、1和2 mL/kg饲粮的剂量喷洒在基础饲粮(30%粗蛋白质)上，制备3组饲粮。试验组采用3 × 2因子设计(3水平茴香油;0,1,2 ml) × 2水平的溶解氧(DO)浓度。实验分为对照无大茴香(高DO)、1 ml +(高DO)、2 ml +(高DO)、对照0 ml(低DO)、1 ml大茴香(低DO)和2 ml大茴香(低DO)。按3%体重连续饲喂60 d。采食量以饲喂前总采食量减去容器内剩余采食量的方法记录每日采食量。结果表明，与对照组相比，鱼饲八角显著提高了生长性能，降低了饲料系数。胴体组成表明，每kg日粮粗蛋白质显著提高2.5和3.5 g。综上所述，在尼罗罗非鱼的饲料中添加大茴香可提高其生产性能和健康状况。
{"title":"Effect of Anise Essential Oil Supplementation on Growth Performance of Nile Tilapia Reared at Low Dissolved Oxygen Level","authors":"Marawan Hussain, Mohamed Nossair, Heba Farag, Alaa Mansour","doi":"10.5455/ajvs.153229","DOIUrl":"https://doi.org/10.5455/ajvs.153229","url":null,"abstract":"Medicinal herbs are the most utilized and validated feed additives in the aquaculture industry. This study investigated the dietary effects of anise (Pimpinella anisum L.) in Nile Tilapia (Oreochromis niloticus). Three sets of diets were prepared by spraying Anise essential oil on a commercial basal diet (30% crude protein) at the rate of 0 (control), 1, and 2 mL/kg diet. The experimental groups were assigned a 3 × 2 factorial design (3 level of anise oil; 0, 1, 2 ml) × 2 levels of dissolved oxygen (DO) concentration. The experiment was assembled as a control no anise (high DO), 1 ml + (high DO), 2 ml + (high DO), Control 0 ml (low DO), 1 ml anise (low DO), and 2 ml anise (low DO). Fish were fed the diets for 60 days consecutively at 3% body weight. The feed intake was recorded daily by subtracting the remaining feed in the containers from the total amount of feed before feeding. The results showed a marked enhancement in the growth performance and reduced feed conversion ratio in fish-fed anise compared to the control group. The carcass composition showed that crude protein was meaningfully by 2.5 and 3.5 g anise per kg diet. In conclusion, incorporating anise into the diet of Nile Tilapia is suggested to enhance performances and well-being.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134884406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was performed on 24 calves aged about 30-100 days old, fourteen calves were suffering from signs of pulmonary affections including fever, bilateral muco-purulent nasal discharge, cough and abnormal lung sounds upon auscultation and these calves were enrolled as pulmonary affected group. The other ten calves were apparently clinically healthy as proved by clinical examination and were used as a control healthy group. Blood and serum samples were collected from healthy and diseased calves (before and after treatment). Hematological analysis of diseased calves before treatment showed a significant decrease in the value of RBCs and Hb, with no significant changes in the value of PCV between healthy and diseased calves before and after treatment. The total leucocytic count showed a significant increase in its value in diseased calves with significant increase in neutrophils and lymphocyte (%) as compared with healthy ones. Serum biochemical analysis of pulmonary affected calves showed hypoproteinemia, hypoalbuminemia, hypoglycemia, hyponatremia, hypochloremia and hyperkalemia as compared with healthy control calves. Activities of serum AST, ALT showed significant elevations in pulmonary affected calves with no significant changes in levels of total and direct bilirubin as compared with healthy calves. Analysis of acute phase protein of diseased calves before treatment showed a significant increase in values of Haptoglobin (Hp), serum amyloid A (SAA), Ceruloplasmin (Cp) and C - reactive protein (CRP) as compared with their levels after treatment and in healthy calves.
{"title":"Clinical, hematological, serum biochemical, and acute phase protein indices in pulmonary affected calves","authors":"M. Ismail, A. Metwally, Zeinab Khamees, M. A.","doi":"10.5455/ajvs.143086","DOIUrl":"https://doi.org/10.5455/ajvs.143086","url":null,"abstract":"This study was performed on 24 calves aged about 30-100 days old, fourteen calves were suffering from signs of pulmonary affections including fever, bilateral muco-purulent nasal discharge, cough and abnormal lung sounds upon auscultation and these calves were enrolled as pulmonary affected group. The other ten calves were apparently clinically healthy as proved by clinical examination and were used as a control healthy group. Blood and serum samples were collected from healthy and diseased calves (before and after treatment). Hematological analysis of diseased calves before treatment showed a significant decrease in the value of RBCs and Hb, with no significant changes in the value of PCV between healthy and diseased calves before and after treatment. The total leucocytic count showed a significant increase in its value in diseased calves with significant increase in neutrophils and lymphocyte (%) as compared with healthy ones. Serum biochemical analysis of pulmonary affected calves showed hypoproteinemia, hypoalbuminemia, hypoglycemia, hyponatremia, hypochloremia and hyperkalemia as compared with healthy control calves. Activities of serum AST, ALT showed significant elevations in pulmonary affected calves with no significant changes in levels of total and direct bilirubin as compared with healthy calves. Analysis of acute phase protein of diseased calves before treatment showed a significant increase in values of Haptoglobin (Hp), serum amyloid A (SAA), Ceruloplasmin (Cp) and C - reactive protein (CRP) as compared with their levels after treatment and in healthy calves.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"2 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78631659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ibrahim Elkamash, M. Said, Mansour Aboelneen, S. Zaabel
The aim of the current study was to use ultrasound imaging to follow the follicular dynamics in dairy cows and to determine the relationship between the diameter of the dominant follicle (DF), the life span of the corpus luteum (CL), interovulatory interval (IOI), and the number of follicular waves. Twenty nine Holstein normal cyclic cows 60 days postpartum were used in this study. The ovaries of the experimental cows were scanned for the DF and CL every three days from ovulation to ovulation. Cows undergo the Ovsynch program to unify the beginning of the ovulation per cycle. The results revealed that, cows that exhibit the two wave patterns have a longer time of IOI than a cow with a one wave pattern (P<0.05), and a shorter length of IOI than cows with three follicular wave patterns (P<0.05). The duration of the DF growth phase in the first follicular wave (Wave 1) was prolonged in a cow that exhibited a one-wave pattern than in the cows that exhibited the two or three-wave patterns (p<0.05). The maximum diameter of DF for a single follicular wave pattern (17.6 ± 0.67 mm). The maximum diameter of the anovulatory follicle in the two-wave patterns (12 ± 2.53 mm) was larger than the maximum diameter of the anovulatory follicle (10.75 ±1.33 mm, p<0.05) of wave one in cows that exhibited three-wave patterns. The lifespan of the corpus luteum in the one-wave pattern was significantly shorter (p<0.05) than in two-wave patterns and three-wave patterns. The length of the IOI was positively increased with the number of the follicular wave and the length of the luteal phase but was negatively related to the diameter of the first dominant follicle in the wave and the duration of the (DF) growth phase in the first wave (p<0.05). In Conclusion, these results demonstrate that the length of the IOI in cows exhibiting the two-wave patterns is shorter than in cows exhibiting three-wave patterns due to the larger diameter of the dominant follicle in wave one, the longer duration of the DF growth phase in the first wave, and the shorter life span of the CL in the cows exhibiting two-wave patterns than in cows exhibiting three-wave patterns.
本研究的目的是利用超声成像技术跟踪奶牛卵泡的动态变化，确定优势卵泡直径(DF)、黄体寿命(CL)、排卵间隔(IOI)与卵泡波数之间的关系。本研究选用29头产后60天正常周期的荷斯坦奶牛。从排卵期到排卵期，每隔3天对试验奶牛卵巢进行DF和CL扫描。奶牛通过ovsync程序来统一每个周期的排卵开始时间。结果表明，两种卵泡波型奶牛的卵泡期比一种卵泡波型奶牛长(P<0.05)，比三种卵泡波型奶牛短(P<0.05)。单波型奶牛的DF生长期在第一卵泡波(波1)中的持续时间比二波型和三波型奶牛的延长(p<0.05)。单个滤泡波形的DF最大直径(17.6±0.67 mm)。两波型奶牛无排卵卵泡最大直径(12±2.53 mm)大于三波型奶牛一波型无排卵卵泡最大直径(10.75±1.33 mm, p<0.05)。黄体单波组的寿命明显短于双波组和三波组(p<0.05)。IOI长度与卵泡波数和黄体期长度呈正相关，而与第一波优势卵泡直径和第一波(DF)生长期持续时间呈负相关(p<0.05)。综上所述，由于第一波优势卵泡直径较大，第一波DF生长期持续时间较长，双波奶牛的IOI长度比三波奶牛短，且双波奶牛的CL寿命比三波奶牛短。
{"title":"The Use of Ultrasound for Monitoring the Relationship Between the Diameter of the Dominant Follicle, The Inter-ovulatory Interval, and Follicular Waves in Dairy Cattle","authors":"Ibrahim Elkamash, M. Said, Mansour Aboelneen, S. Zaabel","doi":"10.5455/ajvs.155956","DOIUrl":"https://doi.org/10.5455/ajvs.155956","url":null,"abstract":"The aim of the current study was to use ultrasound imaging to follow the follicular dynamics in dairy cows and to determine the relationship between the diameter of the dominant follicle (DF), the life span of the corpus luteum (CL), interovulatory interval (IOI), and the number of follicular waves. Twenty nine Holstein normal cyclic cows 60 days postpartum were used in this study. The ovaries of the experimental cows were scanned for the DF and CL every three days from ovulation to ovulation. Cows undergo the Ovsynch program to unify the beginning of the ovulation per cycle. The results revealed that, cows that exhibit the two wave patterns have a longer time of IOI than a cow with a one wave pattern (P<0.05), and a shorter length of IOI than cows with three follicular wave patterns (P<0.05). The duration of the DF growth phase in the first follicular wave (Wave 1) was prolonged in a cow that exhibited a one-wave pattern than in the cows that exhibited the two or three-wave patterns (p<0.05). The maximum diameter of DF for a single follicular wave pattern (17.6 ± 0.67 mm). The maximum diameter of the anovulatory follicle in the two-wave patterns (12 ± 2.53 mm) was larger than the maximum diameter of the anovulatory follicle (10.75 ±1.33 mm, p<0.05) of wave one in cows that exhibited three-wave patterns. The lifespan of the corpus luteum in the one-wave pattern was significantly shorter (p<0.05) than in two-wave patterns and three-wave patterns. The length of the IOI was positively increased with the number of the follicular wave and the length of the luteal phase but was negatively related to the diameter of the first dominant follicle in the wave and the duration of the (DF) growth phase in the first wave (p<0.05). In Conclusion, these results demonstrate that the length of the IOI in cows exhibiting the two-wave patterns is shorter than in cows exhibiting three-wave patterns due to the larger diameter of the dominant follicle in wave one, the longer duration of the DF growth phase in the first wave, and the shorter life span of the CL in the cows exhibiting two-wave patterns than in cows exhibiting three-wave patterns.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76261974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study aimed to assess the effect of dietary supplementation of DL- Methionine (DL-Meth) on growth performance and health of growing Japanese quails. A total of 300, 2- week-old quails were randomly divided into 4 equal experimental groups, with 8 replicates of 10 quails per pen, for 4 weeks. Quails in the control group were fed a control diet whereas in the other experimental groups the quails were fed a diet supplemented with DL-Meth (0.99%) at 1, 3, and 5g/kg diet. Quail’s performance traits in each experimental group were calculated weekly. At the end of the growth period, eight quails (one/replicate) from each group were chosen randomly for serum collection, carcass attributes measurement. The results revealed that quails’ growth traits; final weight (FW), weight gain (WG), and feed conversion ratio (FCR) showed a significant improvement (p < .05) (linear) as DL-Meth supplementation level increased to 3 and 5g/kg diet (7.2 and 9.1g/kg Meth respectively). Serum superoxide dismutase and catalase activities were improved (p < .05) in quails fed DL-Meth supplemented diet at 3, and 5g/kg. DL-Meth supplementation at 3, and 5g/kg was associated with a significant (p < .05) increase in quails’ breast muscle percentages compared to the other groups. In addition, the sensory evaluation of meat was not influenced by supplementation of methionine at different levels. It is concluded that under our experimental conditions, supplementation of 3g/kg DL- Meth (methionine content, 7.2g/kg) to corn-soybean meal-based diet for Japanese quails, was appropriate for enhancing the birds’ growth, breast muscle yield, and antioxidant activity with maintaining the acceptable sensory quality of birds’ meat.
{"title":"Effect of DL Methionine Supplementation in Japanese Quail Diets on Growth, Meat Quality and Health","authors":"R. Mahmoud, H. Ghanem, S. Salem","doi":"10.5455/ajvs.147511","DOIUrl":"https://doi.org/10.5455/ajvs.147511","url":null,"abstract":"The present study aimed to assess the effect of dietary supplementation of DL- Methionine (DL-Meth) on growth performance and health of growing Japanese quails. A total of 300, 2- week-old quails were randomly divided into 4 equal experimental groups, with 8 replicates of 10 quails per pen, for 4 weeks. Quails in the control group were fed a control diet whereas in the other experimental groups the quails were fed a diet supplemented with DL-Meth (0.99%) at 1, 3, and 5g/kg diet. Quail’s performance traits in each experimental group were calculated weekly. At the end of the growth period, eight quails (one/replicate) from each group were chosen randomly for serum collection, carcass attributes measurement. The results revealed that quails’ growth traits; final weight (FW), weight gain (WG), and feed conversion ratio (FCR) showed a significant improvement (p < .05) (linear) as DL-Meth supplementation level increased to 3 and 5g/kg diet (7.2 and 9.1g/kg Meth respectively). Serum superoxide dismutase and catalase activities were improved (p < .05) in quails fed DL-Meth supplemented diet at 3, and 5g/kg. DL-Meth supplementation at 3, and 5g/kg was associated with a significant (p < .05) increase in quails’ breast muscle percentages compared to the other groups. In addition, the sensory evaluation of meat was not influenced by supplementation of methionine at different levels. It is concluded that under our experimental conditions, supplementation of 3g/kg DL- Meth (methionine content, 7.2g/kg) to corn-soybean meal-based diet for Japanese quails, was appropriate for enhancing the birds’ growth, breast muscle yield, and antioxidant activity with maintaining the acceptable sensory quality of birds’ meat.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72921077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amr Seify, I. Samaha, Mohammad A. Nossair, Sahar Lami
A total of 200 random samples of processed chicken meat products including frankfurter, luncheon, strips, and nuggets (50 of each) were collected from different supermarkets at Alexandria province. The samples were examined bacteriologically immediately after arrival to the laboratory for isolation and identification of some pathogenic bacteria that may contaminate these products as E. coli, Salmonellae, Campylobacter jejuni, and Staphylococcus aureus. The obtained results revealed that the incidence of E. coli in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 14, 6 and 0%, respectively. It was observed that the highest incidence was recorded in luncheon followed by frankfurter and lastly nuggets, the incidence of Salmonellae in the examined samples was 4, 6, 0 and 0%, respectively. The incidence of Y. enterocolitica in the examined samples of frankfurter, luncheon, strips, and nuggets was 4, 8, 2, and 2%, respectively. The incidence of C. jejuni in the examined samples of frankfurter, luncheon, strips, and nuggets was 6, 8, 0, and 2%, respectively. The incidence of S. aureus in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 8, 6, and 6%, respectively. Based on the recorded results in the current study, it was clear that the rates of isolation of the investigated enteric bacteria were higher in chicken frankfurter and luncheon as compared to strips and nuggets that may be attributed to the hygienic conditions of the working places and the awareness of the workers.
{"title":"Incidence of Enteric Pathogens in Cooked Poultry Products in Relation to Public Health in Alexandria Province","authors":"Amr Seify, I. Samaha, Mohammad A. Nossair, Sahar Lami","doi":"10.5455/ajvs.107272","DOIUrl":"https://doi.org/10.5455/ajvs.107272","url":null,"abstract":"A total of 200 random samples of processed chicken meat products including frankfurter, luncheon, strips, and nuggets (50 of each) were collected from different supermarkets at Alexandria province. The samples were examined bacteriologically immediately after arrival to the laboratory for isolation and identification of some pathogenic bacteria that may contaminate these products as E. coli, Salmonellae, Campylobacter jejuni, and Staphylococcus aureus. The obtained results revealed that the incidence of E. coli in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 14, 6 and 0%, respectively. It was observed that the highest incidence was recorded in luncheon followed by frankfurter and lastly nuggets, the incidence of Salmonellae in the examined samples was 4, 6, 0 and 0%, respectively. The incidence of Y. enterocolitica in the examined samples of frankfurter, luncheon, strips, and nuggets was 4, 8, 2, and 2%, respectively. The incidence of C. jejuni in the examined samples of frankfurter, luncheon, strips, and nuggets was 6, 8, 0, and 2%, respectively. The incidence of S. aureus in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 8, 6, and 6%, respectively. Based on the recorded results in the current study, it was clear that the rates of isolation of the investigated enteric bacteria were higher in chicken frankfurter and luncheon as compared to strips and nuggets that may be attributed to the hygienic conditions of the working places and the awareness of the workers.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79966212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bovine viral diarrhea (BVD) is an economically significant diseases in the bovine industry causing severe economic losses and there is no specific treatment so the primary aim of control to detect and remove persistently infected cattle (PI carriers) which serve as a virus source by shedding the virus life along and responsible for the presence of the virus within herds. So early diagnosis of PI animal is the critical point. To our knowledge, there have been no reports about the disease’s situation in New valley province, Egypt. The goal of this study was to serodiagnosis of BVD infection by Serum Neutralization Test (SNT) and early diagnosis of PI animals using BVDV Ag (POC)Test and Double antibody Sandwich ELISA (DS. ELISA) .In this study a total of 320 cattle of different ages, sexes, and breeds (not previously vaccinated with BVD vaccines) were screened clinically for symptoms of BVD infection, 92 cattle out of the totally screened 320 cattle showed clinical signs arousing suspicion of BVD( respiratory manifestation, diarrhea, growth retardation, oral lesion, and abortion) and undergo SNT and the result shows the percent of infection was 31.5% (29/ 92) .20 animal out of 92 clinically diseased undergo BVDV Ag Point-of-Care Test for detection of PI animal, 11 out of 20 examined cattle (55 %) were positive. PI animal was found to be high in cattle aged>2 months – 6 months than in other age groups. Also, male cattle had a greater infection rate than female ones, and infection was high in Frisian than in native bread. For more accurate confirmation five positive PI animal undergo (DS. ELISA). All Five positive PI animal were positive in ELISA (100% compatibility).
牛病毒性腹泻(BVD)是牛工业中经济上重要的疾病，造成严重的经济损失，没有特定的治疗方法，因此控制的主要目的是发现和清除持续感染的牛(PI携带者)，它们通过释放病毒生命并导致病毒在牛群中存在而成为病毒源。因此早期诊断是PI动物的关键。据我们所知，没有关于埃及新谷省该病情况的报告。本研究的目的是通过血清中和试验(SNT)诊断BVD感染，并采用BVDV Ag (POC)试验和双抗体夹心ELISA (DS)对PI动物进行早期诊断。本研究对320头不同年龄、性别、品种(未接种BVD疫苗)的牛进行了BVD感染的临床筛查，其中92头牛出现了可疑的BVD临床症状(呼吸症状、腹泻、生长迟缓、口腔病变、腹泻)。结果表明，92头临床患病的20头牛中，有11头(55%)的牛进行了BVDV Ag定点检测(Point-of-Care Test)。结果表明，0 ~ 2 ~ 6月龄牛的PI水平高于其他年龄组。此外，公牛的感染率高于母牛，弗里斯兰面包的感染率高于本地面包。为了更准确的确认，5只PI阳性的动物接受DS。ELISA)。5只PI阳性动物ELISA均为阳性(100%相容性)。
{"title":"Serodiagnosis and Detection of Persistently Infected Cattle with BVDV in New Valley Province, Egypt","authors":"A. Maher, O. Hakim, M. Saad, Yasser F. Elnaker","doi":"10.5455/ajvs.144953","DOIUrl":"https://doi.org/10.5455/ajvs.144953","url":null,"abstract":"Bovine viral diarrhea (BVD) is an economically significant diseases in the bovine industry causing severe economic losses and there is no specific treatment so the primary aim of control to detect and remove persistently infected cattle (PI carriers) which serve as a virus source by shedding the virus life along and responsible for the presence of the virus within herds. So early diagnosis of PI animal is the critical point. To our knowledge, there have been no reports about the disease’s situation in New valley province, Egypt. The goal of this study was to serodiagnosis of BVD infection by Serum Neutralization Test (SNT) and early diagnosis of PI animals using BVDV Ag (POC)Test and Double antibody Sandwich ELISA (DS. ELISA) .In this study a total of 320 cattle of different ages, sexes, and breeds (not previously vaccinated with BVD vaccines) were screened clinically for symptoms of BVD infection, 92 cattle out of the totally screened 320 cattle showed clinical signs arousing suspicion of BVD( respiratory manifestation, diarrhea, growth retardation, oral lesion, and abortion) and undergo SNT and the result shows the percent of infection was 31.5% (29/ 92) .20 animal out of 92 clinically diseased undergo BVDV Ag Point-of-Care Test for detection of PI animal, 11 out of 20 examined cattle (55 %) were positive. PI animal was found to be high in cattle aged>2 months – 6 months than in other age groups. Also, male cattle had a greater infection rate than female ones, and infection was high in Frisian than in native bread. For more accurate confirmation five positive PI animal undergo (DS. ELISA). All Five positive PI animal were positive in ELISA (100% compatibility).","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81682529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Roshdy, R. Khalil, Hani latif, T. Saad, M. Tanekhy
This study aimed to determine and identify the common bacterial infection in different stages of cultured sea bass during the different season. In addition Eexperimentally infected the sea bass with the Vibrio alginolyticus and Vibrio parahemolyticus. The aquarium trial was carried out with European seabass (Dicentrarchus labrax) in private fish farm at Borg El-Arab, Alexandria governorate Egypt. At total 50 of seabass was used during the study. The study lasted 10 weeks, starting with 50 ± 5 gram /fish. Prior to the start of the trial, the fish were fed with a commercial diet (45 percent CP, crude protein). Water quality parameters were monitored during fish acclimatization and throughout the trial. Water temperature, pH and dissolved oxygen remained within acceptable ranges for marine production during the trial. Our study concluded that, Vibrio alginolyticus and Vibrio parahemolyticus causes a great losses in cluttered sea bass Dicentrarchus Labrax reared in cages The presence of histopathological lesions in the internal organs clearly explained the septicemic nature of Vibrio infection. Altogether, the full confirmation of Vibrio infection ought to incorporate, examination of fishes for the visible clinical signs, postmortem examination, isolation of the causative agent using specific media and biochemical tests, and histopathological examination of the internal organs as a proof of septicemic nature of the Vibrio infection. The sensitivity test revealed sensitive to ciprofloxacin (Cipro 5μg), novobiocine (NV 30 μg), intermediate sensitivity was found to Oxytetracycline (OT 30 μg) while, the resistance was observed to amoxicillin (Aml 10 μg) and colisitine sulphate (CT 10 μg)
{"title":"Diseases problems in cluttered sea bass Dicentrarchus Labrax reared in cages from Fingerlings until marketing size in Mariout Valley at King Mariout in Alexandria province","authors":"M. Roshdy, R. Khalil, Hani latif, T. Saad, M. Tanekhy","doi":"10.5455/ajvs.126197","DOIUrl":"https://doi.org/10.5455/ajvs.126197","url":null,"abstract":"This study aimed to determine and identify the common bacterial infection in different stages of cultured sea bass during the different season. In addition Eexperimentally infected the sea bass with the Vibrio alginolyticus and Vibrio parahemolyticus. The aquarium trial was carried out with European seabass (Dicentrarchus labrax) in private fish farm at Borg El-Arab, Alexandria governorate Egypt. At total 50 of seabass was used during the study. The study lasted 10 weeks, starting with 50 ± 5 gram /fish. Prior to the start of the trial, the fish were fed with a commercial diet (45 percent CP, crude protein). Water quality parameters were monitored during fish acclimatization and throughout the trial. Water temperature, pH and dissolved oxygen remained within acceptable ranges for marine production during the trial. Our study concluded that, Vibrio alginolyticus and Vibrio parahemolyticus causes a great losses in cluttered sea bass Dicentrarchus Labrax reared in cages The presence of histopathological lesions in the internal organs clearly explained the septicemic nature of Vibrio infection. Altogether, the full confirmation of Vibrio infection ought to incorporate, examination of fishes for the visible clinical signs, postmortem examination, isolation of the causative agent using specific media and biochemical tests, and histopathological examination of the internal organs as a proof of septicemic nature of the Vibrio infection. The sensitivity test revealed sensitive to ciprofloxacin (Cipro 5μg), novobiocine (NV 30 μg), intermediate sensitivity was found to Oxytetracycline (OT 30 μg) while, the resistance was observed to amoxicillin (Aml 10 μg) and colisitine sulphate (CT 10 μg)","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85532989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present work, two experiments were conducted to investigate the efficiency of supplementing buck sperm medium with Moringa oleifera leaves extract on liquid-chilled storage and cryopreservation capacities of spermatozoa. Twenty ejaculates were collected by an artificial vagina from 5 Damascus bucks, 4 ejaculates each, during September-October. The pooled specimens of each collection session were diluted (1:10) with Tris-citric egg yolk extender, and were split into 6 equal aliquots. The first aliquot served as control (moringa-free), whereas the other five aliquots were supplemented with 0.05, 0.1, 0.2, 0.3 or 0.4 mL/ml (v/v) moringa leaves extract, respectively. The samples were then stored and maintained at 4 oC for the subsequent 48 h during which sperm traits were assessed alongside malondialdehyde (MDA) production and glutathione peroxidase (GPX) activity. The optimum concentration of moringa leaves extract that showed potential for maintaining sperm traits throughout the chilled storage period was further investigated for preserving the lifespan of spermatozoa after exposure to the cryopreservation stress against control. The results showed that moringa leaves extract supplementation reduced (P<0.05) the oxidative stress on spermatozoa and recorded a positive correlation (P<0.01) with sperm motility (%), viability (%), normal sperm (%), acrosome integrity and GPX activity (r= 0.36, 0.42, 0.61, 0.79 and 0.35, respectively) while recording a negative correlation (P<0.01) with production of MDA (r= - 0.56). Furthermore, supplementing buck sperm medium with 0.4 mL/ml moringa leaves extract efficiently improved (P<0.05) all sperm characteristics and decreased (P<0.05) DNA fragmentation index (DFI) post thawing compared to those of control. These results imply the powerful potential of moringa leaves extract as an exogenous antioxidant supplement in buck sperm medium particularly when spermatozoa are used for AI and IVFM applications.
{"title":"Improving Capacities of Chilled- and Cryo-Preserved Buck Spermatozoa by Enriching Preservation Medium with Moringa oleifera leaves extract","authors":"M. Khalifa","doi":"10.5455/ajvs.146334","DOIUrl":"https://doi.org/10.5455/ajvs.146334","url":null,"abstract":"In the present work, two experiments were conducted to investigate the efficiency of supplementing buck sperm medium with Moringa oleifera leaves extract on liquid-chilled storage and cryopreservation capacities of spermatozoa. Twenty ejaculates were collected by an artificial vagina from 5 Damascus bucks, 4 ejaculates each, during September-October. The pooled specimens of each collection session were diluted (1:10) with Tris-citric egg yolk extender, and were split into 6 equal aliquots. The first aliquot served as control (moringa-free), whereas the other five aliquots were supplemented with 0.05, 0.1, 0.2, 0.3 or 0.4 mL/ml (v/v) moringa leaves extract, respectively. The samples were then stored and maintained at 4 oC for the subsequent 48 h during which sperm traits were assessed alongside malondialdehyde (MDA) production and glutathione peroxidase (GPX) activity. The optimum concentration of moringa leaves extract that showed potential for maintaining sperm traits throughout the chilled storage period was further investigated for preserving the lifespan of spermatozoa after exposure to the cryopreservation stress against control. The results showed that moringa leaves extract supplementation reduced (P<0.05) the oxidative stress on spermatozoa and recorded a positive correlation (P<0.01) with sperm motility (%), viability (%), normal sperm (%), acrosome integrity and GPX activity (r= 0.36, 0.42, 0.61, 0.79 and 0.35, respectively) while recording a negative correlation (P<0.01) with production of MDA (r= - 0.56). Furthermore, supplementing buck sperm medium with 0.4 mL/ml moringa leaves extract efficiently improved (P<0.05) all sperm characteristics and decreased (P<0.05) DNA fragmentation index (DFI) post thawing compared to those of control. These results imply the powerful potential of moringa leaves extract as an exogenous antioxidant supplement in buck sperm medium particularly when spermatozoa are used for AI and IVFM applications.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"140 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86738071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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