Eman R. Hassan, Hossam A. Ibrahim, Michael M. Shawky, G. Hamad, M. Wahab
Recently, researchers have been more interested in natural than synthetic antioxidants because consumers and industry prefer foods with natural antioxidants to synthetic ones. During past two decades, many researchers tested essential oils like morenga and wheat germ because they contain chemical compounds that can increase or enhance shelf-life of foods, make them more resistant to oxidation, slow down the microbial or fungal growth, and show positive effect on their physiochemical properties. Therefore, the aim of this study was to evaluate the antioxidant and antimicrobial effect of two essential oils (morenga and wheat germ) to be used as natural preservative in Chicken fillet, So they were applied as natural preservative in Chicken fillet at three concentrations (0.2%, 0.5% and 1%) and compared with negative control and positive control (BHT) and the TBARS, DNPH, pH value, WHC and microbial load was evaluated through a storage period of 12 days and at 4 °C, during storage the treatment showed a results superior to that of the synthetic antioxidant with a lower TBARS value, carbonyl content and microbial load. These findings show that this essential oils at the level of 1% is very effective against lipid and protein oxidation, and a powerful antimicrobial in chicken fillet and is a promising natural antioxidant and antimicrobial replacing the synthetic preservatives in meat processing
{"title":"Influence of Natural Antioxidants from Essential Oils on Shelf Life of Chicken Fillet","authors":"Eman R. Hassan, Hossam A. Ibrahim, Michael M. Shawky, G. Hamad, M. Wahab","doi":"10.5455/ajvs.131448","DOIUrl":"https://doi.org/10.5455/ajvs.131448","url":null,"abstract":"Recently, researchers have been more interested in natural than synthetic antioxidants because consumers and industry prefer foods with natural antioxidants to synthetic ones. During past two decades, many researchers tested essential oils like morenga and wheat germ because they contain chemical compounds that can increase or enhance shelf-life of foods, make them more resistant to oxidation, slow down the microbial or fungal growth, and show positive effect on their physiochemical properties. Therefore, the aim of this study was to evaluate the antioxidant and antimicrobial effect of two essential oils (morenga and wheat germ) to be used as natural preservative in Chicken fillet, So they were applied as natural preservative in Chicken fillet at three concentrations (0.2%, 0.5% and 1%) and compared with negative control and positive control (BHT) and the TBARS, DNPH, pH value, WHC and microbial load was evaluated through a storage period of 12 days and at 4 °C, during storage the treatment showed a results superior to that of the synthetic antioxidant with a lower TBARS value, carbonyl content and microbial load. These findings show that this essential oils at the level of 1% is very effective against lipid and protein oxidation, and a powerful antimicrobial in chicken fillet and is a promising natural antioxidant and antimicrobial replacing the synthetic preservatives in meat processing","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"415 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84898581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bovine viral diarrhea (BVD) is an economically significant diseases in the bovine industry causing severe economic losses and there is no specific treatment so the primary aim of control to detect and remove persistently infected cattle (PI carriers) which serve as a virus source by shedding the virus life along and responsible for the presence of the virus within herds. So early diagnosis of PI animal is the critical point. To our knowledge, there have been no reports about the disease’s situation in New valley province, Egypt. The goal of this study was to serodiagnosis of BVD infection by Serum Neutralization Test (SNT) and early diagnosis of PI animals using BVDV Ag (POC)Test and Double antibody Sandwich ELISA (DS. ELISA) .In this study a total of 320 cattle of different ages, sexes, and breeds (not previously vaccinated with BVD vaccines) were screened clinically for symptoms of BVD infection, 92 cattle out of the totally screened 320 cattle showed clinical signs arousing suspicion of BVD( respiratory manifestation, diarrhea, growth retardation, oral lesion, and abortion) and undergo SNT and the result shows the percent of infection was 31.5% (29/ 92) .20 animal out of 92 clinically diseased undergo BVDV Ag Point-of-Care Test for detection of PI animal, 11 out of 20 examined cattle (55 %) were positive. PI animal was found to be high in cattle aged>2 months – 6 months than in other age groups. Also, male cattle had a greater infection rate than female ones, and infection was high in Frisian than in native bread. For more accurate confirmation five positive PI animal undergo (DS. ELISA). All Five positive PI animal were positive in ELISA (100% compatibility).
牛病毒性腹泻(BVD)是牛工业中经济上重要的疾病，造成严重的经济损失，没有特定的治疗方法，因此控制的主要目的是发现和清除持续感染的牛(PI携带者)，它们通过释放病毒生命并导致病毒在牛群中存在而成为病毒源。因此早期诊断是PI动物的关键。据我们所知，没有关于埃及新谷省该病情况的报告。本研究的目的是通过血清中和试验(SNT)诊断BVD感染，并采用BVDV Ag (POC)试验和双抗体夹心ELISA (DS)对PI动物进行早期诊断。本研究对320头不同年龄、性别、品种(未接种BVD疫苗)的牛进行了BVD感染的临床筛查，其中92头牛出现了可疑的BVD临床症状(呼吸症状、腹泻、生长迟缓、口腔病变、腹泻)。结果表明，92头临床患病的20头牛中，有11头(55%)的牛进行了BVDV Ag定点检测(Point-of-Care Test)。结果表明，0 ~ 2 ~ 6月龄牛的PI水平高于其他年龄组。此外，公牛的感染率高于母牛，弗里斯兰面包的感染率高于本地面包。为了更准确的确认，5只PI阳性的动物接受DS。ELISA)。5只PI阳性动物ELISA均为阳性(100%相容性)。
{"title":"Serodiagnosis and Detection of Persistently Infected Cattle with BVDV in New Valley Province, Egypt","authors":"A. Maher, O. Hakim, M. Saad, Yasser F. Elnaker","doi":"10.5455/ajvs.144953","DOIUrl":"https://doi.org/10.5455/ajvs.144953","url":null,"abstract":"Bovine viral diarrhea (BVD) is an economically significant diseases in the bovine industry causing severe economic losses and there is no specific treatment so the primary aim of control to detect and remove persistently infected cattle (PI carriers) which serve as a virus source by shedding the virus life along and responsible for the presence of the virus within herds. So early diagnosis of PI animal is the critical point. To our knowledge, there have been no reports about the disease’s situation in New valley province, Egypt. The goal of this study was to serodiagnosis of BVD infection by Serum Neutralization Test (SNT) and early diagnosis of PI animals using BVDV Ag (POC)Test and Double antibody Sandwich ELISA (DS. ELISA) .In this study a total of 320 cattle of different ages, sexes, and breeds (not previously vaccinated with BVD vaccines) were screened clinically for symptoms of BVD infection, 92 cattle out of the totally screened 320 cattle showed clinical signs arousing suspicion of BVD( respiratory manifestation, diarrhea, growth retardation, oral lesion, and abortion) and undergo SNT and the result shows the percent of infection was 31.5% (29/ 92) .20 animal out of 92 clinically diseased undergo BVDV Ag Point-of-Care Test for detection of PI animal, 11 out of 20 examined cattle (55 %) were positive. PI animal was found to be high in cattle aged>2 months – 6 months than in other age groups. Also, male cattle had a greater infection rate than female ones, and infection was high in Frisian than in native bread. For more accurate confirmation five positive PI animal undergo (DS. ELISA). All Five positive PI animal were positive in ELISA (100% compatibility).","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81682529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present work, two experiments were conducted to investigate the efficiency of supplementing buck sperm medium with Moringa oleifera leaves extract on liquid-chilled storage and cryopreservation capacities of spermatozoa. Twenty ejaculates were collected by an artificial vagina from 5 Damascus bucks, 4 ejaculates each, during September-October. The pooled specimens of each collection session were diluted (1:10) with Tris-citric egg yolk extender, and were split into 6 equal aliquots. The first aliquot served as control (moringa-free), whereas the other five aliquots were supplemented with 0.05, 0.1, 0.2, 0.3 or 0.4 mL/ml (v/v) moringa leaves extract, respectively. The samples were then stored and maintained at 4 oC for the subsequent 48 h during which sperm traits were assessed alongside malondialdehyde (MDA) production and glutathione peroxidase (GPX) activity. The optimum concentration of moringa leaves extract that showed potential for maintaining sperm traits throughout the chilled storage period was further investigated for preserving the lifespan of spermatozoa after exposure to the cryopreservation stress against control. The results showed that moringa leaves extract supplementation reduced (P<0.05) the oxidative stress on spermatozoa and recorded a positive correlation (P<0.01) with sperm motility (%), viability (%), normal sperm (%), acrosome integrity and GPX activity (r= 0.36, 0.42, 0.61, 0.79 and 0.35, respectively) while recording a negative correlation (P<0.01) with production of MDA (r= - 0.56). Furthermore, supplementing buck sperm medium with 0.4 mL/ml moringa leaves extract efficiently improved (P<0.05) all sperm characteristics and decreased (P<0.05) DNA fragmentation index (DFI) post thawing compared to those of control. These results imply the powerful potential of moringa leaves extract as an exogenous antioxidant supplement in buck sperm medium particularly when spermatozoa are used for AI and IVFM applications.
{"title":"Improving Capacities of Chilled- and Cryo-Preserved Buck Spermatozoa by Enriching Preservation Medium with Moringa oleifera leaves extract","authors":"M. Khalifa","doi":"10.5455/ajvs.146334","DOIUrl":"https://doi.org/10.5455/ajvs.146334","url":null,"abstract":"In the present work, two experiments were conducted to investigate the efficiency of supplementing buck sperm medium with Moringa oleifera leaves extract on liquid-chilled storage and cryopreservation capacities of spermatozoa. Twenty ejaculates were collected by an artificial vagina from 5 Damascus bucks, 4 ejaculates each, during September-October. The pooled specimens of each collection session were diluted (1:10) with Tris-citric egg yolk extender, and were split into 6 equal aliquots. The first aliquot served as control (moringa-free), whereas the other five aliquots were supplemented with 0.05, 0.1, 0.2, 0.3 or 0.4 mL/ml (v/v) moringa leaves extract, respectively. The samples were then stored and maintained at 4 oC for the subsequent 48 h during which sperm traits were assessed alongside malondialdehyde (MDA) production and glutathione peroxidase (GPX) activity. The optimum concentration of moringa leaves extract that showed potential for maintaining sperm traits throughout the chilled storage period was further investigated for preserving the lifespan of spermatozoa after exposure to the cryopreservation stress against control. The results showed that moringa leaves extract supplementation reduced (P<0.05) the oxidative stress on spermatozoa and recorded a positive correlation (P<0.01) with sperm motility (%), viability (%), normal sperm (%), acrosome integrity and GPX activity (r= 0.36, 0.42, 0.61, 0.79 and 0.35, respectively) while recording a negative correlation (P<0.01) with production of MDA (r= - 0.56). Furthermore, supplementing buck sperm medium with 0.4 mL/ml moringa leaves extract efficiently improved (P<0.05) all sperm characteristics and decreased (P<0.05) DNA fragmentation index (DFI) post thawing compared to those of control. These results imply the powerful potential of moringa leaves extract as an exogenous antioxidant supplement in buck sperm medium particularly when spermatozoa are used for AI and IVFM applications.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"140 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86738071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nihal Abdelkader, M. Kammar, H. Ahmed, Ahmed Korritum
A retrospective study of corneo-conjunctival disorders was conducted on 71 dogs at several private pet clinics in Alexandria in Egypt between 2019 and 2022 to determine the incidence of corneo-conjunctival disorders among different breeds, ages, and sexes of dogs using descriptive statistical tools. The overall incidence of corneo-conjunctival disorders in dogs was (35.50%) of the total ocular disorders encountered with Golden retriever breed being the most affected. The majority of corneo-conjunctival affections were met with dogs less than 1 year of age with the cornea being the most affected anatomical location. Corneal Ulcer was the most frequently diagnosed clinical condition. It was also seen that males were affected more than females.
{"title":"Clinical And Surgical Assessments of Disorders of Conjunctiva and Cornea in Dogs: A Retrospective Study","authors":"Nihal Abdelkader, M. Kammar, H. Ahmed, Ahmed Korritum","doi":"10.5455/ajvs.151681","DOIUrl":"https://doi.org/10.5455/ajvs.151681","url":null,"abstract":"A retrospective study of corneo-conjunctival disorders was conducted on 71 dogs at several private pet clinics in Alexandria in Egypt between 2019 and 2022 to determine the incidence of corneo-conjunctival disorders among different breeds, ages, and sexes of dogs using descriptive statistical tools. The overall incidence of corneo-conjunctival disorders in dogs was (35.50%) of the total ocular disorders encountered with Golden retriever breed being the most affected. The majority of corneo-conjunctival affections were met with dogs less than 1 year of age with the cornea being the most affected anatomical location. Corneal Ulcer was the most frequently diagnosed clinical condition. It was also seen that males were affected more than females.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86988075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amr Seify, I. Samaha, Mohammad A. Nossair, Sahar Lami
A total of 200 random samples of processed chicken meat products including frankfurter, luncheon, strips, and nuggets (50 of each) were collected from different supermarkets at Alexandria province. The samples were examined bacteriologically immediately after arrival to the laboratory for isolation and identification of some pathogenic bacteria that may contaminate these products as E. coli, Salmonellae, Campylobacter jejuni, and Staphylococcus aureus. The obtained results revealed that the incidence of E. coli in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 14, 6 and 0%, respectively. It was observed that the highest incidence was recorded in luncheon followed by frankfurter and lastly nuggets, the incidence of Salmonellae in the examined samples was 4, 6, 0 and 0%, respectively. The incidence of Y. enterocolitica in the examined samples of frankfurter, luncheon, strips, and nuggets was 4, 8, 2, and 2%, respectively. The incidence of C. jejuni in the examined samples of frankfurter, luncheon, strips, and nuggets was 6, 8, 0, and 2%, respectively. The incidence of S. aureus in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 8, 6, and 6%, respectively. Based on the recorded results in the current study, it was clear that the rates of isolation of the investigated enteric bacteria were higher in chicken frankfurter and luncheon as compared to strips and nuggets that may be attributed to the hygienic conditions of the working places and the awareness of the workers.
{"title":"Incidence of Enteric Pathogens in Cooked Poultry Products in Relation to Public Health in Alexandria Province","authors":"Amr Seify, I. Samaha, Mohammad A. Nossair, Sahar Lami","doi":"10.5455/ajvs.107272","DOIUrl":"https://doi.org/10.5455/ajvs.107272","url":null,"abstract":"A total of 200 random samples of processed chicken meat products including frankfurter, luncheon, strips, and nuggets (50 of each) were collected from different supermarkets at Alexandria province. The samples were examined bacteriologically immediately after arrival to the laboratory for isolation and identification of some pathogenic bacteria that may contaminate these products as E. coli, Salmonellae, Campylobacter jejuni, and Staphylococcus aureus. The obtained results revealed that the incidence of E. coli in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 14, 6 and 0%, respectively. It was observed that the highest incidence was recorded in luncheon followed by frankfurter and lastly nuggets, the incidence of Salmonellae in the examined samples was 4, 6, 0 and 0%, respectively. The incidence of Y. enterocolitica in the examined samples of frankfurter, luncheon, strips, and nuggets was 4, 8, 2, and 2%, respectively. The incidence of C. jejuni in the examined samples of frankfurter, luncheon, strips, and nuggets was 6, 8, 0, and 2%, respectively. The incidence of S. aureus in the examined samples of frankfurter, luncheon, strips, and nuggets was 10, 8, 6, and 6%, respectively. Based on the recorded results in the current study, it was clear that the rates of isolation of the investigated enteric bacteria were higher in chicken frankfurter and luncheon as compared to strips and nuggets that may be attributed to the hygienic conditions of the working places and the awareness of the workers.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79966212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study aimed to assess the effect of dietary supplementation of DL- Methionine (DL-Meth) on growth performance and health of growing Japanese quails. A total of 300, 2- week-old quails were randomly divided into 4 equal experimental groups, with 8 replicates of 10 quails per pen, for 4 weeks. Quails in the control group were fed a control diet whereas in the other experimental groups the quails were fed a diet supplemented with DL-Meth (0.99%) at 1, 3, and 5g/kg diet. Quail’s performance traits in each experimental group were calculated weekly. At the end of the growth period, eight quails (one/replicate) from each group were chosen randomly for serum collection, carcass attributes measurement. The results revealed that quails’ growth traits; final weight (FW), weight gain (WG), and feed conversion ratio (FCR) showed a significant improvement (p < .05) (linear) as DL-Meth supplementation level increased to 3 and 5g/kg diet (7.2 and 9.1g/kg Meth respectively). Serum superoxide dismutase and catalase activities were improved (p < .05) in quails fed DL-Meth supplemented diet at 3, and 5g/kg. DL-Meth supplementation at 3, and 5g/kg was associated with a significant (p < .05) increase in quails’ breast muscle percentages compared to the other groups. In addition, the sensory evaluation of meat was not influenced by supplementation of methionine at different levels. It is concluded that under our experimental conditions, supplementation of 3g/kg DL- Meth (methionine content, 7.2g/kg) to corn-soybean meal-based diet for Japanese quails, was appropriate for enhancing the birds’ growth, breast muscle yield, and antioxidant activity with maintaining the acceptable sensory quality of birds’ meat.
{"title":"Effect of DL Methionine Supplementation in Japanese Quail Diets on Growth, Meat Quality and Health","authors":"R. Mahmoud, H. Ghanem, S. Salem","doi":"10.5455/ajvs.147511","DOIUrl":"https://doi.org/10.5455/ajvs.147511","url":null,"abstract":"The present study aimed to assess the effect of dietary supplementation of DL- Methionine (DL-Meth) on growth performance and health of growing Japanese quails. A total of 300, 2- week-old quails were randomly divided into 4 equal experimental groups, with 8 replicates of 10 quails per pen, for 4 weeks. Quails in the control group were fed a control diet whereas in the other experimental groups the quails were fed a diet supplemented with DL-Meth (0.99%) at 1, 3, and 5g/kg diet. Quail’s performance traits in each experimental group were calculated weekly. At the end of the growth period, eight quails (one/replicate) from each group were chosen randomly for serum collection, carcass attributes measurement. The results revealed that quails’ growth traits; final weight (FW), weight gain (WG), and feed conversion ratio (FCR) showed a significant improvement (p < .05) (linear) as DL-Meth supplementation level increased to 3 and 5g/kg diet (7.2 and 9.1g/kg Meth respectively). Serum superoxide dismutase and catalase activities were improved (p < .05) in quails fed DL-Meth supplemented diet at 3, and 5g/kg. DL-Meth supplementation at 3, and 5g/kg was associated with a significant (p < .05) increase in quails’ breast muscle percentages compared to the other groups. In addition, the sensory evaluation of meat was not influenced by supplementation of methionine at different levels. It is concluded that under our experimental conditions, supplementation of 3g/kg DL- Meth (methionine content, 7.2g/kg) to corn-soybean meal-based diet for Japanese quails, was appropriate for enhancing the birds’ growth, breast muscle yield, and antioxidant activity with maintaining the acceptable sensory quality of birds’ meat.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72921077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramota Lawal, Tagang Aluwong, Musa Suleiman, Lateef Adeniran, Monday Onakpa, Peter Ajagbonna
Diabetes mellitus is a significant public health problem worldwide. Oxidative stress plays a pivotal role in the pathogenesis of diabetes as it is one of the inevitable outcomes of the cellular process. The present study aims to investigate the putative antihyperglycemic, antihyperlipidemic and antioxidant efficacy of limonene and vitamin E compared with metformin, a standard drug for the therapy of diabetes. Diabetes was induced by a single intraperitoneal injection of 150 mg/kg of alloxan. There was a significant (P<0.05) increase in body weight of rats treated with limonene, vitamin E, and combinatory therapy of limonene and vitamin E compared to the untreated diabetic group which showed a decrease in weight. Combinatory therapy treatment of limonene and vitamin E showed a significant difference (P<0.05) when compared with the groups treated with limonene, vitamin E, and metformin. The combinatory therapy also reduced blood glucose levels, significantly (P < 0.05) compared to the non-diabetic treated group treated with limonene and the group treated with vitamin E. Also, the combined treated group ameliorated the effect of diabetes on total cholesterol, triglyceride concentration, high-density lipoprotein, and low-density lipoprotein. Treatment with limonene and vitamin E, significantly (P< 0.05) ameliorated diabetic effect on MDA concentration and SOD activity. Pathological lesions observed in the pancreas treated with limonene, vitamin E, and combinatory therapy of limonene and vitamin E showed normal tissue architecture compared to the group treated with metformin. It was concluded that limonene and vitamin E exerted significant anti-diabetic and anti-hyperglycaemic effects in alloxan-induced diabetic rats.
{"title":"D-Limonene and Vitamin E Combinatory Therapy Stimulate Erythropoiesis and Repair Of Β-Cells Atrophy in Alloxan–Induced Diabetic Rats","authors":"Ramota Lawal, Tagang Aluwong, Musa Suleiman, Lateef Adeniran, Monday Onakpa, Peter Ajagbonna","doi":"10.5455/ajvs.106781","DOIUrl":"https://doi.org/10.5455/ajvs.106781","url":null,"abstract":"Diabetes mellitus is a significant public health problem worldwide. Oxidative stress plays a pivotal role in the pathogenesis of diabetes as it is one of the inevitable outcomes of the cellular process. The present study aims to investigate the putative antihyperglycemic, antihyperlipidemic and antioxidant efficacy of limonene and vitamin E compared with metformin, a standard drug for the therapy of diabetes. Diabetes was induced by a single intraperitoneal injection of 150 mg/kg of alloxan. There was a significant (P<0.05) increase in body weight of rats treated with limonene, vitamin E, and combinatory therapy of limonene and vitamin E compared to the untreated diabetic group which showed a decrease in weight. Combinatory therapy treatment of limonene and vitamin E showed a significant difference (P<0.05) when compared with the groups treated with limonene, vitamin E, and metformin. The combinatory therapy also reduced blood glucose levels, significantly (P < 0.05) compared to the non-diabetic treated group treated with limonene and the group treated with vitamin E. Also, the combined treated group ameliorated the effect of diabetes on total cholesterol, triglyceride concentration, high-density lipoprotein, and low-density lipoprotein. Treatment with limonene and vitamin E, significantly (P< 0.05) ameliorated diabetic effect on MDA concentration and SOD activity. Pathological lesions observed in the pancreas treated with limonene, vitamin E, and combinatory therapy of limonene and vitamin E showed normal tissue architecture compared to the group treated with metformin. It was concluded that limonene and vitamin E exerted significant anti-diabetic and anti-hyperglycaemic effects in alloxan-induced diabetic rats.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"156 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135261762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Areej Shehata, Mostafa Kassem, Mahmoud Kammar, Howaida Ahmed
The present anesthetic investigation was aimed to evaluate anesthetic clinico-physiological and hematobiochemical effects in addition to the anesthetic properties of propofol or ketamine premedicated with diazepam . For this purpose, 12 apparently healthy dogs were randomly divided into Two groups: A and B. All dogs were premedicated with Diazepam (0.2mg/kg Bwt/IM) then intravenous anesthesia was induced in group A with propofol intravenous (1mg/kg Bwt) ,while group B with ketamine intravenous (5 mg/kg Bwt). Blood samples were collected from the Two groups 15 minutes before anesthesia induction, then at (15, 30, 45 and 60) minutes after treatments for the hemato-biochemical evaluation, and for clinico-physiological observations. A slight alteration in some physiological parameters and hemato-biochemical values was observed after anesthesia induction, but it still safe to induce general anesthesia in dogs. in conclusion, the present anesthetic investigation showed the longest period of anesthesia with complete muscle relaxation following intravenous ketamine injection than propofol in premedicated dogs with diazepam.
{"title":"Anesthetic and clinical assessments of I/V propofol versus ketamine in Dogs premedicated with diazepam","authors":"Areej Shehata, Mostafa Kassem, Mahmoud Kammar, Howaida Ahmed","doi":"10.5455/ajvs.159481","DOIUrl":"https://doi.org/10.5455/ajvs.159481","url":null,"abstract":"The present anesthetic investigation was aimed to evaluate anesthetic clinico-physiological and hematobiochemical effects in addition to the anesthetic properties of propofol or ketamine premedicated with diazepam . For this purpose, 12 apparently healthy dogs were randomly divided into Two groups: A and B. All dogs were premedicated with Diazepam (0.2mg/kg Bwt/IM) then intravenous anesthesia was induced in group A with propofol intravenous (1mg/kg Bwt) ,while group B with ketamine intravenous (5 mg/kg Bwt). Blood samples were collected from the Two groups 15 minutes before anesthesia induction, then at (15, 30, 45 and 60) minutes after treatments for the hemato-biochemical evaluation, and for clinico-physiological observations. A slight alteration in some physiological parameters and hemato-biochemical values was observed after anesthesia induction, but it still safe to induce general anesthesia in dogs. in conclusion, the present anesthetic investigation showed the longest period of anesthesia with complete muscle relaxation following intravenous ketamine injection than propofol in premedicated dogs with diazepam.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135447653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study, a total of 273 commercial Cobb chicks were subjectively divided into seven groups to evaluate the effects of a probiotic mixture, prebiotic (Isomaltooligosaccharide), and their combination additions on growth performance, intestinal microbiome, caecal colonisation of Salmonella of broilers challenged with S. enteritidis and S. typhimurium. The groups included G01: basal diet (negative control), G02; basal diet and challenged with Salmonella on 3rd day of life (positive control), G03: received probiotic mixture in drinking water and then challenged with Salmonella, G04: received prebiotic in feed and then orally challenged with Salmonella, G05: received probiotic mix and prebiotic (synbiotic) and then challenged with Salmonella, G06: received probiotic mix in drinking water, and G07: received prebiotic in the feed. The obtained data exhibited that probiotic mixture, prebiotic, and synbiotic supplements noteworthy, upgraded growth performance, reduced total Salmonella counts, and augmented the total numbers of Lactobacillus and Enterococcus in the caeca of challenged birds compared with those of challenge untreated group. Compared to the probiotic mix and prebiotic single supplements, the synbiotic effects were notable. Also, probiotic mixture and prebiotic supplements significantly enhanced growth performance and adjusted the caecum microbial composition of uninfected birds compared with those of the uninfected untreated group. In conclusion, probiotic mixture, prebiotic, and in particular, synbiotic supplements could enhance growth performance and increase Lactobacillus and Enterococcus population counts in caeca of broilers experimentally challenged with S. enteritidis and S. typhimurium.
{"title":"Effects of Probiotics and Prebiotics and their Combinations on Growth Performance and Intestinal Microbiota of Broilers Infected with Mixed Salmonellae","authors":"R. Tarabees, S. A., A. S, D. A, EL A, Gaber M","doi":"10.5455/ajvs.143849","DOIUrl":"https://doi.org/10.5455/ajvs.143849","url":null,"abstract":"In the present study, a total of 273 commercial Cobb chicks were subjectively divided into seven groups to evaluate the effects of a probiotic mixture, prebiotic (Isomaltooligosaccharide), and their combination additions on growth performance, intestinal microbiome, caecal colonisation of Salmonella of broilers challenged with S. enteritidis and S. typhimurium. The groups included G01: basal diet (negative control), G02; basal diet and challenged with Salmonella on 3rd day of life (positive control), G03: received probiotic mixture in drinking water and then challenged with Salmonella, G04: received prebiotic in feed and then orally challenged with Salmonella, G05: received probiotic mix and prebiotic (synbiotic) and then challenged with Salmonella, G06: received probiotic mix in drinking water, and G07: received prebiotic in the feed. The obtained data exhibited that probiotic mixture, prebiotic, and synbiotic supplements noteworthy, upgraded growth performance, reduced total Salmonella counts, and augmented the total numbers of Lactobacillus and Enterococcus in the caeca of challenged birds compared with those of challenge untreated group. Compared to the probiotic mix and prebiotic single supplements, the synbiotic effects were notable. Also, probiotic mixture and prebiotic supplements significantly enhanced growth performance and adjusted the caecum microbial composition of uninfected birds compared with those of the uninfected untreated group. In conclusion, probiotic mixture, prebiotic, and in particular, synbiotic supplements could enhance growth performance and increase Lactobacillus and Enterococcus population counts in caeca of broilers experimentally challenged with S. enteritidis and S. typhimurium.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74554617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanim S Heikal, Hanan Elsyd, Rania Tahan, H. Mahboub
Abstract: The aim of this work was to evaluate the impact of crossing pure Gimmizah or Bandara (local breeds) with pure Ross (a foreign breed) on growth rate, and the expression of IGF genes. Two local breeds, Gimmizah (G) and Bandara (B), plus one exotic strain of meat-type Ross 308 (R) parents, were used. Combinations of these genotypes were tested on a total of 120 birds from three pure parents. The 180 hatched chicks were divided into three purebreds G×G, B×B, and R×R and three crossbreds R×G, R×B, and G×R and reared for 7 weeks. Body weight and weight gain were recorded for each genetic group. The feed intake and feed conversion ratio were calculated. At the 7th week of age, tissue samples from breast muscle and pituitary gland were collected for IGF gene mRNA expression analysis. The results revealed that the R×R chicks displayed significantly higher final body weight, average daily weight gain, and average daily feed intake when compared to the G×G, B×B, and other crossbred chick lines. While both R×B and G×R crossbred chicks had significantly higher final body weights and average daily weight gains than R×G crossbred chicks and local chicks (G×G and B×B). A better feed conversion ratio was observed in G×R, B×B, R×R and R×B chicks. Crossbred lines (R×B and G×R) had higher dressing percentages than R×G and local breeds. The highest muscular expression of the IGF-II gene was recorded in R×G chicks, while the other crossbred lines, R×B and G×R expressed higher levels of the IGF-II gene than purebred lines. In relation to IGF-I*, R×B chicks had higher relative expression of this gene than other crossbred and purebred lines. When compared to other crossbred lines (R×B and G×R), the expression of IGF-I in brain tissues was significantly higher in the R×G crossbred line. IGF-II and IGF-I* expression levels in brain tissue were higher in the R×G chicks compared to the G×R, G×G, B×B, and R×R chicks. It was concluded that crossing pure Gimmizah or Bandara, as local breeds, with pure Ross 308, as a foreign breed, improved growth traits in the obtained crossbred chicks.
{"title":"Impact of Crossbreeding Between Local and Foreign Strains on the Growth Traits and the Expression of Insulin-Like Growth Factor Genes on Their Chicks","authors":"Hanim S Heikal, Hanan Elsyd, Rania Tahan, H. Mahboub","doi":"10.5455/ajvs.138355","DOIUrl":"https://doi.org/10.5455/ajvs.138355","url":null,"abstract":"Abstract: The aim of this work was to evaluate the impact of crossing pure Gimmizah or Bandara (local breeds) with pure Ross (a foreign breed) on growth rate, and the expression of IGF genes. Two local breeds, Gimmizah (G) and Bandara (B), plus one exotic strain of meat-type Ross 308 (R) parents, were used. Combinations of these genotypes were tested on a total of 120 birds from three pure parents. The 180 hatched chicks were divided into three purebreds G×G, B×B, and R×R and three crossbreds R×G, R×B, and G×R and reared for 7 weeks. Body weight and weight gain were recorded for each genetic group. The feed intake and feed conversion ratio were calculated. At the 7th week of age, tissue samples from breast muscle and pituitary gland were collected for IGF gene mRNA expression analysis. The results revealed that the R×R chicks displayed significantly higher final body weight, average daily weight gain, and average daily feed intake when compared to the G×G, B×B, and other crossbred chick lines. While both R×B and G×R crossbred chicks had significantly higher final body weights and average daily weight gains than R×G crossbred chicks and local chicks (G×G and B×B). A better feed conversion ratio was observed in G×R, B×B, R×R and R×B chicks. Crossbred lines (R×B and G×R) had higher dressing percentages than R×G and local breeds. The highest muscular expression of the IGF-II gene was recorded in R×G chicks, while the other crossbred lines, R×B and G×R expressed higher levels of the IGF-II gene than purebred lines. In relation to IGF-I*, R×B chicks had higher relative expression of this gene than other crossbred and purebred lines. When compared to other crossbred lines (R×B and G×R), the expression of IGF-I in brain tissues was significantly higher in the R×G crossbred line. IGF-II and IGF-I* expression levels in brain tissue were higher in the R×G chicks compared to the G×R, G×G, B×B, and R×R chicks. It was concluded that crossing pure Gimmizah or Bandara, as local breeds, with pure Ross 308, as a foreign breed, improved growth traits in the obtained crossbred chicks.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"372 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72615218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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