Bioresource Technology Reports最新文献_第9页

Circular bioeconomy in the bioproducts industry: Utilizing cocoa and coffee husk as inert supports for the production of Trichoderma koningiopsis (Tricotec® WP) 生物制品行业的循环生物经济：利用可可和咖啡壳作为惰性载体生产柯宁质木霉（Tricotec®WP）

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-24 DOI: 10.1016/j.biteb.2025.102497

Vanessa Chavarro-Anzola , Marcela Franco-Correa , Mauricio Cruz-Barrera

The concept of circular bioeconomy has emerged as a sustainable strategy to addressing waste management, environmental degradation, and the demand for renewable resources. In the bioproducts industry, applying these principles enables the transformation of agricultural waste into valuable bio-based products. This study explores the potential of cocoa and coffee husks-bioproducts of the chocolate and coffee industries- as inert substrates for the production of Trichoderma koningiopsis - Th003, a beneficial fungus used in biocontrol and biofertilization. The research aims to assess the suitability of cocoa and coffee husks as growth substrates for T. koningiopsis – Th003. The husks underwent pre-treatment processes to remove contaminants and enhance their properties. Subsequently, T. koningiopsis- Th003 was inoculated on the treated husks and cultivated under controlled laboratory conditions. Fungal growth kinetics, biomass production, and viability were monitored throughout the process. The highest conidial concentration achieved was

1.12 \times 10^{9}

conidia/g in cocoa husks and

4.30 \times 10^{8}

conidia/g in coffee husks, comparable to the standard substrate (rice with wheat bran). Utilizing these agro-industrial waste materials as substrates for T. koningiopsis-Th003 let to production cost savings of up to 20 %. A second fermentation cycle with the waste materials yielded

2 \times 10^{9}

conidia/g in coffee husks, leading to savings of up to 50 %. These findings demonstrated the potential of coffee and cocoa husks as promising alternatives to cereal-based substrates for production active ingredients.

循环生物经济的概念已成为解决废物管理、环境退化和对可再生资源需求的可持续战略。在生物制品行业，应用这些原则可以将农业废物转化为有价值的生物基产品。本研究探索了可可和咖啡壳——巧克力和咖啡工业的生物制品——作为生产克宁木霉Th003的惰性底物的潜力，这是一种用于生物防治和生物肥料的有益真菌。本研究旨在评估可可和咖啡壳作为T. koningiopsis - Th003生长基质的适宜性。稻壳经过预处理以去除污染物并增强其性能。随后，将T. koningiopsis- Th003接种在处理过的谷壳上，并在实验室控制条件下进行培养。在整个过程中监测真菌生长动力学，生物量生产和活力。在可可壳和咖啡壳中获得的最高分生孢子浓度分别为1.12×109conidia/g和4.30×108conidia/g，与标准底物（稻谷加麦麸）相当。利用这些农用工业废料作为T. koningiopsis-Th003的底物，可以节省高达20%的生产成本。在第二次发酵循环中，废物在咖啡壳中产生2×109conidia/g，从而节省高达50%。这些发现证明了咖啡和可可壳作为谷类基基质生产活性成分的有希望的替代品的潜力。

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引用次数: 0

Marine cyanobacteria Leptolyngbya sp. nov. (KMBMA-1) extract as biofertilizer for Vigna uniguiculata and Solanum lycopersicum 海洋蓝藻leptolyynbya sp. 11 （KMBMA-1）提取物作为紫菜和番茄茄的生物肥料

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-24 DOI: 10.1016/j.biteb.2025.102486

C.C. Manjumol , V.P. Limna Mol , K.G. Nevin

Marine cyanobacteria are gaining attention as sustainable biofertilizer resources due to their bioactive metabolites and nutrient-rich biomass. This study evaluated the growth-promoting potential of a novel marine cyanobacterial strain, Leptolyngbya sp. nov. KMBMA-1, on Vigna unguiculata (cowpea) and Solanum lycopersicum (tomato). The seeds treated with cyanobacterial biomass showed significantly higher growth compared to the controls. By Day 21, in comparison with the control plants (39 cm and 18 ± 1 cm), cowpea and tomato plants recorded higher lengths (52 cm and 25 ± 2 cm) with increased growth rate (45.3 % and 38.9 %, respectively). FTIR analysis revealed hydroxyl, carbonyl, and other biomolecule-associated functional groups, which may contribute to growth enhancement. XRD confirmed the semi-crystalline nature of the biomass, consistent with polysaccharides and proteins. Plant growth was improved with reduced soil nitrogen and phosphorus concentrations in the treated pots, indicating more efficient nutrient uptake. Overall, Leptolyngbya sp. nov. KMBMA-1 demonstrated promising biofertilizer potential, supporting improved crop performance and reduced dependence on synthetic fertilizers.

海洋蓝藻因其生物活性代谢物和丰富的生物量而成为可持续的生物肥料资源。本研究评估了一种新的海洋蓝藻菌株leptolyynbya sp. nov. KMBMA-1在豇豆和番茄上的促生长潜力。与对照相比，用蓝藻生物量处理的种子表现出显著更高的生长。第21天，与对照植株（39 cm和18±1 cm）相比，豇豆和番茄植株（52 cm和25±2 cm）长得更高，生长速率分别提高了45.3%和38.9%。FTIR分析显示羟基、羰基和其他生物分子相关官能团可能有助于促进生长。XRD证实了生物质的半结晶性质，与多糖和蛋白质一致。处理后的盆栽土壤氮磷浓度降低，植株生长得到改善，表明养分吸收效率提高。总体而言，leptolyynbya sp. 11 . KMBMA-1显示出很好的生物肥料潜力，支持提高作物性能和减少对合成肥料的依赖。

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引用次数: 0

The CO2 gradient adaptive laboratory evolution of a self-flocculating Desertifilum sp. in wastewater for nutrient removal and phycocyanin production 自絮凝荒漠菌在废水中去除营养物和生产藻蓝蛋白的CO2梯度适应性实验室进化

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-23 DOI: 10.1016/j.biteb.2025.102498

Wanqing Ding , Wenbiao Jin , Xu Zhou , Dan Kang , Tong Li , Yixiang Miao , Xuan Li

This study first reported a newly self-flocculating cyanobacteria Desertifilum sp. for nutrient removal, photosynthetic carbon fixation, and phycocyanin production in municipal wastewater and 15 % CO₂ through CO₂ gradient adaptive laboratory evolution (ALE). The performance of the starting strain AS was evaluated at different levels of CO₂, and results showed the strain could tolerate 5 % CO₂. The strain was subsequently domesticated using ALE over eight rounds, with the CO₂ concentration progressively increased from 8 % to 15 %. This procedure resulted in the domesticated strain 15SR5, which exhibited high CO₂ tolerance. The biomass and carbon fixation rate reached 2.175 g/L and 0.975 g/L/d, and the removal of TN and TP reached 80.60 % and 89.03 %, respectively, within 4 days. The ALE process did not affect the strain's good sedimentation and phycocyanin production. The domesticated strain 15SR5 exhibited enhanced photosynthetic activities and antioxidant properties compared to the starting strain AS. This study contributes to a novel cyanobacterial candidate for nitrogen and phosphorus removal and phycocyanin production, supporting the industrial applications of microalgae or cyanobacteria in wastewater treatment.

本研究首次报道了一种新的自絮凝蓝藻（Desertifilum sp.），通过CO2梯度适应实验室进化（ALE）在城市污水和15% CO2中去除营养物质、光合固定碳和生产藻蓝蛋白。对起始菌株AS在不同CO2浓度下的性能进行了评价，结果表明该菌株可耐受5% CO2。该菌株随后使用ALE驯化了8轮，CO₂浓度从8%逐渐增加到15%。这一过程产生了驯化菌株15SR5，该菌株表现出较高的CO2耐受性。4 d的生物量和固碳率分别达到2.175 g/L和0.975 g/L/d， TN和TP的去除率分别达到80.60%和89.03%。ALE工艺不影响菌株的良好沉降和藻蓝蛋白的生产。驯化菌株15SR5的光合活性和抗氧化性能均高于起始菌株AS。该研究为脱氮除磷和生产藻蓝蛋白提供了一种新的蓝藻候选菌，支持了微藻或蓝藻在废水处理中的工业应用。

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引用次数: 0

Biodegradation of nicosulfuron by dominant strain HX06: Degradation characteristics, pathway, and potential for soil remediation 优势菌株HX06对尼科磺隆的生物降解：降解特性、途径和土壤修复潜力

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-23 DOI: 10.1016/j.biteb.2025.102489

Jiale Zhang , Xin Wang , Wanlei Yue , Jia Bao , Mengqin Yao

Nicotinsulfuron, a widely used sulfonylurea herbicide, poses increasing risks of soil contamination and ecological disruption due to its persistence in agricultural settings. To reduce the environmental impact of this compound, a dominant nicotinsulfuron-degrading strain, designated HX06, was isolated from farmland with a history of long-term nicotinsulfuron use and identified as Aspergillus fumigatus. Degradation conditions were optimized using single-factor experiments combined with response surface methodology (RSM). The optimal degradation conditions were determined to be pH 6.79, inoculum volume of 6.09 %, and incubation temperature of 32.02 °C, under which the predicted degradation rate reached 77.06 %, closely matching the experimental result of 79.30 % (p > 0.05), thereby validating the model reliability. Strain HX06 demonstrated strong degradation capacity across a concentration range of 50–100 mg/L, achieving 67.83 % degradation in soil within 20 days. Metabolic pathway analysis revealed key intermediates and suggested potential enzymatic mechanisms. Furthermore, bioremediation experiments showed that the use of HX06 effectively mitigated the phytotoxic effects of nicotinsulfuron on crops. Unlike previously reported bacterial degraders, strain HX06 (Aspergillus fumigatus) represents a novel fungal resource exhibiting superior degradation efficiency and broader adaptability under variable conditions. Overall, this study enriches the microbial resources available for herbicide degradation, expands strain resources for bioremediation applications, and provides theoretical support for improving soil quality and advancing sustainable agricultural practices.

尼古丁磺隆是一种广泛使用的磺脲类除草剂，由于其在农业环境中的持久性，造成土壤污染和生态破坏的风险越来越大。为减少该化合物对环境的影响，从长期使用烟碱磺隆的农田中分离出一株烟碱磺隆降解优势菌株HX06，鉴定为烟曲霉。采用单因素试验结合响应面法（RSM）优化降解条件。在pH为6.79、接种量为6.09%、培养温度为32.02℃的条件下，预测降解率达到77.06%，与实验结果79.30%非常接近（p > 0.05），验证了模型的可靠性。菌株HX06在50 ~ 100 mg/L的浓度范围内均表现出较强的降解能力，在20天内对土壤的降解率达到67.83%。代谢途径分析揭示了关键中间体和潜在的酶促机制。此外，生物修复实验表明，HX06的使用有效地减轻了烟碱磺隆对作物的植物毒性作用。与之前报道的细菌降解物不同，菌株HX06（烟曲霉）是一种新型真菌资源，在不同条件下具有优异的降解效率和更广泛的适应性。本研究丰富了用于除草剂降解的微生物资源，拓展了生物修复应用的菌种资源，为改善土壤质量和推进可持续农业实践提供了理论支持。

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引用次数: 0

Supplementing XYR1-mutated Trichoderma reesei strain cultivation with (SO2-ethanol-water) softwood pulp improves cellulase production 添加（so2 -乙醇-水）软木浆培养xyr1突变的里氏木霉可提高纤维素酶的产量

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-23 DOI: 10.1016/j.biteb.2025.102501

Yan Wang , Nina Aro , Minna Yamamoto , Mikhail Iakovlev , Markku Saloheimo , Kaisa Marjamaa , Emma Master , Ossi Turunen , Anna Kankaanpää

The cellulolytic enzyme cost remains a major bottleneck in converting lignocellulose, especially softwoods, into fuels and chemicals. The aim of this study was to evaluate possibilities to increase enzyme production efficiency by using SO₂-ethanol-water (SEW) pretreated softwood pulp with a Trichoderma reesei strain that expresses a mutant form of the main transcriptional regulator, XYR1, of cellulase- and hemicellulase genes leading to loss of glucose repression/carbon catabolite repression. The (hemi)cellulase enzyme cocktail of this strain was improved by expressing three heterologous enzymes, a beta-glucosidase, a CEL6 (CBH2) exoglucanase and a lytic polysaccharide mono‑oxygenase. Seven bioreactor cultivations were performed using glucose and different cellulose supplementations and glucose feed strategies. We showed that adding 3 %-w/v cellulose to the glucose medium and starting the glucose feed when the glucose was consumed from the batch medium, improved the protein production rate by over 80 % during the first five days compared to total absence of cellulose. With only 3 % cellulose addition to the batch phase, we estimate that over one third of time and total carbon source, including cellulose, could be saved compared to a production process without cellulosic substrate supplementation. Additionally, enzymes produced with SEW pulp in 119 h and those produced with glucose alone in 193 h both achieved 90 % glucose conversion when used for SEW pulp hydrolysis at a protein loading of 4–5 mg/g cellulose. Herein, we have shown that the M2883 strain can produce more than 29 FPU/mL of the complete set of cellulase enzymes both with and without cellulose supplementation.

纤维素水解酶的成本仍然是将木质纤维素，特别是软木转化为燃料和化学品的主要瓶颈。本研究的目的是评估使用so2 -乙醇-水（SEW）预处理的软木浆和里氏木霉菌株提高酶生产效率的可能性，该菌株表达纤维素酶和半纤维素酶基因的主要转录调节因子XYR1的突变形式，导致葡萄糖抑制/碳分解代谢抑制丧失。该菌株通过表达β -葡萄糖苷酶、CEL6 （CBH2）外葡聚糖酶和多糖单加氧酶3种异源酶，改善了半纤维素酶混合物。采用葡萄糖和不同的纤维素添加物以及葡萄糖饲料策略进行了7个生物反应器培养。我们发现，在葡萄糖培养基中添加3% -w/v的纤维素，当葡萄糖从批处理培养基中消耗时开始葡萄糖饲料，与完全不添加纤维素相比，在前5天内蛋白质产量提高了80%以上。与不添加纤维素底物的生产过程相比，仅在批处理阶段添加3%的纤维素，我们估计可以节省超过三分之一的时间和总碳源，包括纤维素。此外，当蛋白质负荷为4-5 mg/g纤维素时，用SEW纸浆在119小时内产生的酶和仅用葡萄糖在193小时产生的酶都实现了90%的葡萄糖转化率。在这里，我们已经证明M2883菌株在添加和不添加纤维素的情况下都能产生超过29 FPU/mL的全套纤维素酶。

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引用次数: 0

Utilization of agricultural waste: Fiber extraction and characterization from Zingiber zerumbet Pseudo-stems for eco-friendly textiles 农业废弃物的利用：用于环保纺织品的生姜假茎纤维提取和表征

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-22 DOI: 10.1016/j.biteb.2025.102502

Siti Rohmah , Wiah Wardiningsih , Fatimatuz Zahro , Ryan Rudy , Rr Wiwiek Eka Mulyani , Samuel Martin Pradana , Didin Wahidin

The growing fiber gap and environmental concerns in the textile industry necessitate the exploration of renewable raw materials. This study evaluates Zingiber zerumbet pseudo-stems, an underutilized agricultural by-product, as a sustainable fiber source. The pseudo-stems were processed via 30-day water retting without chemicals to extract fibers, which were then characterized. The fibers exhibited an average length of 62.67 cm, fineness of 4.08 Tex, tenacity of 12.69 gf/Tex, elongation of 5.97 %, coefficient of friction of 0.44, and moisture regain of 11.60 %. SEM analysis revealed rough surfaces with visible lumens, XRD indicated a crystallinity index of 49.07 %, and FTIR confirmed the presence of cellulose, hemicellulose, and lignin. Compared with other natural fibers, Zingiber zerumbet fibers showed favorable fineness, adequate strength, and elongation, meeting industrial thresholds for selected textile and composite applications. These results highlight the potential of valorizing Zingiber zerumbet pseudo-stems as an eco-friendly fiber resource, supporting waste reduction and sustainable textile production.

日益增长的纤维缺口和纺织业对环境的关注使探索可再生原料成为必要。本研究评价了一种未充分利用的农业副产品——生姜假茎作为可持续纤维来源的价值。假茎经过30天无化学物质的水浸处理以提取纤维，然后对其进行表征。纤维的平均长度为62.67 cm，细度为4.08 Tex，韧性为12.69 gf/Tex，伸长率为5.97%，摩擦系数为0.44，回潮率为11.60%。SEM分析显示表面粗糙，可见管腔，XRD显示结晶度指数为49.07%，FTIR证实存在纤维素、半纤维素和木质素。与其他天然纤维相比，生姜纤维具有良好的细度，足够的强度和伸长率，达到了纺织和复合材料应用的工业门槛。这些结果强调了将生姜假茎作为一种环保纤维资源的潜力，支持减少废物和可持续纺织品生产。

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引用次数: 0

Effect of different adjuvants on the biocidal and synergistic potential of four types of ZnO and CuO nanoparticles obtained by biological synthesis 不同佐剂对四种生物合成氧化锌和氧化铜纳米颗粒的杀菌和协同作用的影响

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-22 DOI: 10.1016/j.biteb.2025.102503

Antonio Jesús Muñoz , Celia Martín , Francisco Espínola , Manuel Moya , Encarnación Ruiz

Antibiotic resistance is one of today's most pressing problems. Using adjuvants and combinations of biocidal agents appears to be a potential solution. This study examined the biocidal effects of four types of ZnO and CuO nanoparticles, which were previously synthesized using green chemistry and combined with low-cost, low-toxicity adjuvants: dimethyl sulfoxide (DMSO), polyethylene glycol (PEG), and Na₂SiO₃. The data were analyzed alongside previous results obtained for another adjuvant: polyvinyl alcohol (PVA). Dose reductions ranging from two to 32-fold were achieved with the same inhibitory effect against four strains: B. cereus, E. coli, S. epidermidis, and P. fluorescens. Of the three new adjuvants tested, Na₂SiO₃ performed the best, followed by DMSO and PEG. However, PVA (a benchmark in previous studies) proved to be the most effective overall and was selected for the synergy experiments. Additionally, synergy tests were performed with PVA-adjuvanted nanoparticles in combination with three antibiotics (ciprofloxacin, ampicillin, and itraconazole) against the aforementioned bacteria and an additional yeast (R. mucilaginosa). The results identified 164 inhibitory combinations, which were analyzed based on fractional inhibitory concentration (FIC) and isobolographic analysis. This analysis identified 139 combinations with an FIC index of less than 1. This indicates that the combinations are synergistic and that the initial doses of the biocidal agents can be reduced by up to eight times to achieve the same effect. These nanoparticles and adjuvants have great potential to enhance the biocidal response of known antibiotics.

抗生素耐药性是当今最紧迫的问题之一。使用佐剂和杀菌剂的组合似乎是一个潜在的解决方案。这项研究检测了四种ZnO和CuO纳米颗粒的生物杀灭效果，这四种纳米颗粒是之前用绿色化学方法合成的，并与低成本、低毒的佐剂：二甲亚砜（DMSO）、聚乙二醇（PEG）和Na₂SiO₃结合使用。数据与先前获得的另一种佐剂聚乙烯醇（PVA）的结果一起进行分析。剂量减少2- 32倍，对蜡样芽孢杆菌、大肠杆菌、表皮葡萄球菌和荧光假单胞菌四种菌株具有相同的抑制效果。在测试的三种新佐剂中，Na₂SiO₃的效果最好，其次是DMSO和PEG。然而，PVA（前人研究的基准）被证明是最有效的，并被选中进行协同实验。此外，还进行了pva佐剂纳米颗粒与三种抗生素（环丙沙星、氨苄西林和伊曲康唑）联合对上述细菌和另外一种酵母（粘胶酵母）的协同试验。结果确定了164种抑制组合，并基于分数抑制浓度（FIC）和等密度分析对其进行了分析。该分析确定了139个FIC指数小于1的组合。这表明，这些组合具有协同作用，杀灭生物剂的初始剂量最多可减少8倍，以达到同样的效果。这些纳米颗粒和佐剂在增强已知抗生素的生物杀灭反应方面具有很大的潜力。

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引用次数: 0

Biodegradable plastics: A sustainable solution or an emerging environmental threat? – A comprehensive review 可生物降解塑料：可持续解决方案还是新出现的环境威胁？-全面检讨

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-22 DOI: 10.1016/j.biteb.2025.102491

Manju Rawat , Yati Goel , Saloni Sharma , Santushti Raghubanshi , Ajay Chalotra , Hadas Mamane Steindel , Ankisha Vijay

The growing environmental impact of traditional plastic waste has sparked global efforts to find sustainable alternatives. Biodegradable plastics (BDPs) have emerged as a potential solution and are increasingly used across various sectors, including packaging, agriculture, and healthcare. The environmental and practical implications of BDPs are not yet fully understood. This review, therefore explores whether these materials are eco-friendly or if they are becoming yet another source of plastic waste. It clarifies the terms ‘bio-based’ and ‘biodegradable’, emphasizing that not all bioplastics possess low molecular weight structures capable of readily degrading under natural conditions. This comprehensive review critically examines the types, production methods, degradation mechanisms, and real-world performance of biodegradable plastics such as polylactic acid (PLA), polyhydroxyalkanoates (PHA), and starch-based polymers. Special emphasis is placed on the degradation behaviour of these plastics in composting sites, marine environments, and landfills, along with the factors governing these processes. Parallelly, it addresses broader impacts like the formation of microplastics, ecological damage, and greenwashing advertising techniques. In the concluding section, the article places the evolving uses of BDPs in the context of current market trends. By consolidating existing literature, this review highlights both the robustness and shortcomings of BDPs. It stresses the need for thorough life cycle assessments, improved waste management strategies, and clearer standards to ensure their sustainable use in the circular economy.

传统塑料垃圾对环境的影响越来越大，这促使全球努力寻找可持续的替代品。可生物降解塑料（BDPs）已成为一种潜在的解决方案，并越来越多地应用于包装、农业和医疗保健等各个领域。目前尚未完全了解bdp的环境和实际影响。因此，这篇综述探讨了这些材料是否环保，或者它们是否正在成为另一种塑料废物来源。它澄清了“生物基”和“可生物降解”这两个术语，强调并非所有生物塑料都具有在自然条件下易于降解的低分子量结构。本文综述了生物可降解塑料的种类、生产方法、降解机制和实际性能，如聚乳酸（PLA）、聚羟基烷酸酯（PHA）和淀粉基聚合物。特别强调这些塑料在堆肥场、海洋环境和垃圾填埋场中的降解行为，以及控制这些过程的因素。同时，它还解决了更广泛的影响，如微塑料的形成、生态破坏和绿色广告技术。在结论部分，文章将bdp的不断发展的用途置于当前市场趋势的背景下。通过整合现有文献，本综述强调了bdp的稳健性和缺点。它强调需要进行全面的生命周期评估，改进废物管理战略，制定更明确的标准，以确保它们在循环经济中的可持续利用。

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引用次数: 0

Advanced hydroprocessing technology for sustainable diesel: Hydrotreatment of renewable and fossil feedstocks 可持续柴油的先进加氢处理技术：可再生和化石原料的加氢处理

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-22 DOI: 10.1016/j.biteb.2025.102499

Anzhelika M. Eremeeva , Yulia A. Chumachenko , Artyom F. Khasanov , Ivan L. Oleynik

The energy strategies of most countries of the world predict the biofuel market to grow by 29–33 million tons by 2030. To produce high-quality diesel fuel, it is proposed to add a vegetable oil hydrotreating reactor to a diesel fraction hydrotreating unit followed by a mixing unit. The hydrodeoxygenation of sunflower oil was carried out in a fixed-bed flow reactor at temperature of 380 °C, under a hydrogen pressure of 4.0 MPa and at weight hourly space velocity of 1 h⁻¹. The composition and properties of the “Green Diesel” samples were determined using gas chromatography and analysis methods specified in the standard EN 590:2009. The addition of bio-components Green diesel increases the cetane number of fuel. An optimal blended fuel includes 31 % mono- and 7 % polycyclic aromatic compounds, 31 % iso- and cycloalkanes, and 31 % n-alkanes. An algorithm for determining the optimal individual composition of a mixed fuel has been developed and presented. The GreenDieselReactor software has been developed in the Python programming language with the ability to select raw materials, calculate the parameters of the hydrotreating process and reactor sizes, and determine the mass and energy balance. The proposed enhancement of the hydrotreating plant, when integrated with a reactor of vegetable oil hydroprocessing with a capacity of 250,000 metric tons per year, is capable of producing 214,000 tons of diesel annually.

世界上大多数国家的能源战略预测，到2030年，生物燃料市场将增长2900 - 3300万吨。为了生产高质量的柴油，建议在柴油馏分加氢处理装置上增加一个植物油加氢反应器，然后再增加一个混合装置。在固定床流动反应器中，温度380℃，氢气压力4.0 MPa，重量时空速1 h−1，对葵花籽油进行了加氢脱氧。“绿色柴油”样品的组成和性能采用气相色谱法和标准EN 590:2009中规定的分析方法进行测定。绿色柴油中生物成分的加入提高了燃料的十六烷值。一种最佳混合燃料包括31%的单环芳香族化合物和7%的多环芳香族化合物、31%的异烷烃和环烷烃以及31%的正烷烃。提出了一种确定混合燃料最优组分的算法。GreenDieselReactor软件是用Python编程语言开发的，能够选择原材料，计算加氢处理过程和反应器尺寸的参数，并确定质量和能量平衡。拟议的加氢处理厂的改进，如果与年产能为25万吨的植物油加氢处理反应堆相结合，每年将能够生产21.4万吨柴油。

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引用次数: 0

Solvent-free chemoenzymatic epoxidation of raw orange essential oil: Process development and evaluation of antimicrobial and antiviral activities 原料橙精油的无溶剂化学酶环氧化：抗菌和抗病毒活性的工艺开发和评价

Q1 Environmental Science

Bioresource Technology Reports

Pub Date : 2025-12-22 DOI: 10.1016/j.biteb.2025.102500

Salvador E. Brandolín , Florencia Martinez , María Cecilia Carpinella , José A. Scilipoti , Carolina Merlo , Ivana Magario

Oxygenated terpenes are promising biobased agents for pest control; however, they are typically found at low concentrations in raw essential oils. This work investigates the chemo-enzymatic epoxidation of orange peel oil as a strategy to enhance the oxygenated terpene content of this abundant and cost-effective limonene source. The in situ Prilezhaev formation of peroxy acids was catalyzed by immobilized Candida antarctica lipase B. The effects of solvent type, carboxylic acid identity and concentration, oxidant dosage and addition mode, temperature, as well as catalyst loading, were evaluated in relation to limonene conversion, limonene epoxide yields, and catalyst reusability over 4 h reaction cycles. The solvent-free system outperformed the conventional toluene-based method. While limonene-1,2-epoxide remained stable, the diepoxide underwent hydrolysis forming a mixture of water-miscible hydroxylated dipentene products. However, this side-reaction could be drastically diminished by decreasing the reaction temperature at 20 °C. Under optimized conditions—a molar ratio of 1:0.2:1.3 (limonene:octanoic acid:hydrogen peroxide), the highest biocatalyst loading (20 wt% relative to limonene), and 20 °C—yields of 57 % for limonene-1,2-epoxide and 43 % for limonene diepoxide were achieved and maintained for three catalyst uses declining thereafter. The epoxidized oil exhibited moderate, synergistic antimicrobial activity attributed to its increased limonene-1,2-epoxide content. Moreover, it showed activity against Zika virus and enhanced cytotoxic properties. The elimination of toluene and the direct use of raw orange peel oil–derived limonene drastically reduced the E-factor, contributing to the sustainability and scalability of this synthetic approach.

氧合萜烯是一种很有前途的生物基害虫防治剂；然而，它们通常以低浓度存在于原始精油中。这项工作研究了化学酶环氧化的橙皮油作为一种策略，以提高氧合萜含量丰富和成本效益的柠檬烯来源。研究了固定化南极念珠菌脂肪酶b催化过氧化物酸的原位Prilezhaev生成。考察了溶剂类型、羧酸种类和浓度、氧化剂用量和添加方式、温度和催化剂负载对4 h反应周期内柠檬烯转化率、环氧烯收率和催化剂可重复使用性的影响。无溶剂体系优于传统的基于甲苯的方法。虽然柠檬烯-1,2-环氧化物保持稳定，但二环氧化物经过水解形成与水混溶的羟基化二戊烯产物的混合物。然而，当反应温度降低到20℃时，这种副反应可以大大减弱。在优化的摩尔比为1:0.2:1.3（柠檬烯：辛酸：过氧化氢）的条件下，最高的生物催化剂负载（相对于柠檬烯重量为20%）和20℃的温度下，柠檬烯-1,2-环氧化物的产率达到57%，柠檬烯-二氧化二烯的产率达到43%。环氧化油表现出适度的协同抗菌活性，这是由于它增加了柠檬烯-1,2-环氧化物的含量。此外，它还显示出对寨卡病毒的活性和增强的细胞毒性。消除甲苯和直接使用生橙皮油衍生的柠檬烯大大降低了e因子，有助于这种合成方法的可持续性和可扩展性。

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引用次数: 0