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Moderate thermal post-treatment of digestate to improve biomethane production from agricultural- and food waste 对沼渣进行适度的热后处理,提高农业和食品废弃物的生物甲烷产量
Q1 Environmental Science Pub Date : 2024-06-19 DOI: 10.1016/j.biteb.2024.101887
Mette Axelsson Bjerg , Eva-Maria Ekstrand , Ingrid Sundgren , Sepehr Shakeri Yekta , Jan Moestedt , Annika Björn

The aim of this study was to assess the feasibility of moderate thermal treatment (70 °C for one hour) of digestate in combination with post-digestion targeting residual biomethane potentials from three full-scale biogas plants digesting food waste (FW), agricultural waste (AW) and a mixture of AW and manure (AWM). Dissolved organic carbon (DOC), biomethane production, and digestate quality were investigated. For the study six laboratory-scale continuously stirred tank biogas reactors working as post-digesters, with thermally-treated and non-treated digestate were used. DOC for thermally-treated digestates increased significantly (t-test, p < 0.05); FW-digestate (110–200 %), AW-digestate (24–92 %) and for AWM-digestate (4–73 %). Indexes for corresponding DOC quality showed lower apparent organic molecular weights and decreased aromaticity (with the exception of FW-digestate). Thermal treatment of digestate improved the biomethane production during post-digestion by 21–22 % (FW-digestate) and 9 % (AW-digestate). For AMW-digestate no clear positive effect was observed, most likely due to biogas plant operational process disturbances.

本研究的目的是评估对沼渣进行适度热处理(70 °C,一小时)的可行性,同时针对三个全规模沼气厂消化厨余垃圾(FW)、农业废弃物(AW)以及农业废弃物和粪便混合物(AWM)的残余生物甲烷潜力进行后消化。对溶解有机碳 (DOC)、生物甲烷产量和沼渣质量进行了调查。研究使用了六个实验室规模的连续搅拌罐沼气反应器,这些反应器作为后消化器工作,沼渣经过热处理和未经过热处理。热处理沼渣的 DOC 显著增加(t 检验,p < 0.05);FW-沼渣(110-200 %)、AW-沼渣(24-92 %)和 AWM-沼渣(4-73 %)。相应的 DOC 质量指标显示,表观有机分子量降低,芳香度下降(FW-沼渣除外)。沼渣的热处理提高了后消化过程中的生物甲烷产量,FW-沼渣提高了 21-22%,AW-沼渣提高了 9%。对于 AMW-沼渣,没有观察到明显的积极影响,这很可能是由于沼气厂的运行过程受到干扰。
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引用次数: 0
Prediction of anaerobic degradation kinetics based on substrate composition of lignocellulosic biomass 基于木质纤维素生物质底物成分的厌氧降解动力学预测
Q1 Environmental Science Pub Date : 2024-06-17 DOI: 10.1016/j.biteb.2024.101882
Karim Alrefaey , Jana Schultz , Marvin Scherzinger , Mahmoud A. Nosier , Amr Y. Elbanhawy

This study presents a comprehensive biochemical predictive approach for assessing biogas production kinetics across ten lignocellulosic substrates in batch operation. The methodology employs a range of kinetic and regression models, all grounded in the substrates' chemical composition. Among the kinetic models, the cone model demonstrated superior performance, achieving an average error of 1.67 % in describing biogas production from all substrates. The quadratic Monod type model followed closely, with an error of 1.96 %. Among the regression models, on the other hand, the logistic function model exhibited enhanced predictive capabilities, yielding an average error of 6.02 %, while the Chen and Hashimoto one showed a higher error of 60.54 %. The findings underscore the potential of precise biogas production forecasting and tracking the daily rates of gas generation, rather than solely relying on cumulative gas yields at the end of the process.

本研究提出了一种全面的生化预测方法,用于评估批量操作中十种木质纤维素基质的沼气生产动力学。该方法采用了一系列动力学和回归模型,所有模型均以基质的化学成分为基础。在动力学模型中,圆锥模型表现出卓越的性能,在描述所有基质的沼气产量时,平均误差为 1.67%。二次莫诺模型紧随其后,误差为 1.96%。另一方面,在回归模型中,逻辑函数模型的预测能力更强,平均误差为 6.02%,而 Chen 和 Hashimoto 模型的误差则更大,达到 60.54%。这些发现强调了精确预测沼气产量和跟踪每日产气量的潜力,而不是仅仅依赖于过程结束时的累积产气量。
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引用次数: 0
Microbial enzyme production: Unlocking the potential of agricultural and food waste through solid-state fermentation 微生物酶生产:通过固态发酵释放农业和食品废弃物的潜力
Q1 Environmental Science Pub Date : 2024-06-15 DOI: 10.1016/j.biteb.2024.101880
Ashutosh Khaswal , Santosh Kumar Mishra , Neha Chaturvedi , Sonu Saini , Brett Pletschke , Ramesh Chander Kuhad

Agricultural and food waste materials are rich resources of valuable biomolecules, including proteins, carbohydrates, fats, minerals, fibers, etc. Improper disposal methods such as landfill decay or open burning significantly contributed to environmental pollution and global warming. Therefore, there is an urgent need to explore sustainable approaches for utilizing these residues without harming the natural environment. One promising possibility involves leveraging these residues to cultivate microorganisms for the production of value-added products like enzymes, organic acids, and biofuels. Solid-state fermentation has emerged as a promising practice for producing commercially important enzymes utilizing agricultural crop wastes. This study aims to provide an overview of various agricultural and food wastes utilized in enzyme production through solid-state fermentation. Industrial enzymes such as amylases, xylanases, pectinases, lipases, cellulases, fructosyl-transferases, and lactases are among those explored. The review encompasses a diverse range of microbial commodity capable of utilizing these low-cost underutilized materials for growth and development, ultimately yielding a variety of industrial important enzymes.

农业和食品废弃物是丰富的宝贵生物大分子资源,包括蛋白质、碳水化合物、脂肪、矿物质、纤维等。填埋腐烂或露天焚烧等不当处理方法极大地加剧了环境污染和全球变暖。因此,迫切需要探索可持续的方法,在不损害自然环境的情况下利用这些残留物。利用这些残留物培养微生物,生产酶、有机酸和生物燃料等高附加值产品,是一种很有前景的方法。固态发酵已成为利用农作物废料生产具有重要商业价值的酶的一种有前途的做法。本研究旨在概述通过固态发酵生产酶的各种农业和食品废物。其中探讨了淀粉酶、木聚糖酶、果胶酶、脂肪酶、纤维素酶、果糖转移酶和乳糖酶等工业酶。该综述涵盖了能够利用这些低成本、利用率低的材料进行生长和发育的各种微生物商品,最终产生了各种重要的工业酶。
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引用次数: 0
Ozonation for improved sludge reduction, cytotoxicity and estrogenicity control in anaerobic digestion 在厌氧消化过程中使用臭氧改善污泥减量、细胞毒性和雌激素控制
Q1 Environmental Science Pub Date : 2024-06-13 DOI: 10.1016/j.biteb.2024.101879
Shengkun Dong , Sare Asli , Kyu Hur , Nedal Massalha

Anaerobic digestion (AD) is a common treatment for sludge reduction and stabilization. Simultaneously reductions in sludge chemical oxygen demand (COD), cytotoxicity, and estrogenicity are desired for safer handling and reuse. We investigated the impact, mechanism, and control strategy of ozonation (common sludge biodegradability method) on AD, methane production, and sludge cytotoxicity and estrogenicity modulations. Results revealed that ozone treatment could boost the methane production potential and rate by up to 36.5 and 69.2 %, respectively, but only after partial sludge digestion. Ozone modulated sludge cytotoxicity and estrogenicity, significantly improving this effect (maximum reductions of 28 and 23 %, respectively) by introducing anaerobically digested sludge to the ozonation module due to lowered volatile solids (VS) compared to raw sludge and less competition from the abundant biodegradable organics. Results support introducing ozone during AD for simultaneous COD reduction, and cytotoxicity and estrogenicity control.

厌氧消化(AD)是一种常见的污泥减量和稳定化处理方法。为了更安全地处理和再利用污泥,人们希望同时降低污泥的化学需氧量(COD)、细胞毒性和雌激素。我们研究了臭氧处理(常用的污泥生物降解方法)对厌氧消化率、甲烷产量、污泥细胞毒性和雌激素调节的影响、机理和控制策略。结果表明,臭氧处理可使甲烷产生潜力和甲烷产生率分别提高 36.5% 和 69.2%,但仅限于污泥部分消化之后。臭氧可调节污泥的细胞毒性和雌激素毒性,通过将厌氧消化污泥引入臭氧处理模块,可显著改善这种效果(最大降幅分别为 28% 和 23%),原因是与生污泥相比,厌氧消化污泥的挥发性固形物(VS)更低,来自丰富的可生物降解有机物的竞争更少。研究结果支持在厌氧消化过程中引入臭氧,以同时减少 COD、控制细胞毒性和雌激素。
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引用次数: 0
Screening of pure cultures for their efficiency to convert electricity and CO2 into methane 筛选纯培养物将电能和二氧化碳转化为甲烷的效率
Q1 Environmental Science Pub Date : 2024-06-10 DOI: 10.1016/j.biteb.2024.101875
Benjamin Roessler , Sandra Off , Oliver Arendt , Johannes Gescher

The demand for defossilization and decarbonization resulting from the climate crisis and the lack of storable renewable energy has brought biomethane back into focus. Methanogenic microorganisms can be used to convert renewable energy into biomethane. In a pressurized H-cell reactor, we demonstrate the electroactivity of four different methanogenic archaea under conditions of direct and indirect electromethanogenesis. The cathodes were adjusted to a potential of −300 mV (direct electromethanogenesis) and − 700 mV (indirect electromethanogenesis) relative to a standard hydrogen electrode. At −300 mV, no significant methane formation was observed in all four cultures. At a potential of −700 mV, Methanococcus maripaludis achieved an average daily methane production rate of 61 mmol/m2 per day. The highest methane concentration of 199.4 mmol/m2 and the highest coulombic efficiency of 74.0 % were reached within 72 h by Methanococcus maripaludis.

气候危机导致的去化石和去碳化需求,以及可储存可再生能源的缺乏,使生物甲烷再次成为人们关注的焦点。产甲烷微生物可用于将可再生能源转化为生物甲烷。在加压 H 细胞反应器中,我们展示了四种不同的产甲烷古细菌在直接和间接电生甲烷条件下的电活性。相对于标准氢电极,阴极电位被调节到-300 mV(直接电生甲烷)和-700 mV(间接电生甲烷)。在-300 mV 时,所有四种培养物都没有观察到明显的甲烷形成。在 -700 mV 的电位下,海洋甲烷球菌(Methanococcus maripaludis)的平均日甲烷产生率为 61 mmol/m2/天。海洋甲烷球菌在 72 小时内达到了 199.4 mmol/m2 的最高甲烷浓度和 74.0 % 的最高库仑效率。
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引用次数: 0
Reuse of biogas sludge for the slow-release fertilizer production to improve fertilizer use efficiency and soil fertility 再利用沼气污泥生产缓释肥,提高肥料利用效率和土壤肥力
Q1 Environmental Science Pub Date : 2024-06-07 DOI: 10.1016/j.biteb.2024.101877
Nguyen Xuan Que Vo , Thi Phi Oanh Tran , Pham Thanh Hien Lam , Thi Bich Huyen Trinh

Fertilizer overuse causes negative impacts on the environment and crop yields. Therefore, the production of slow-release fertilizers from biogas sludge is a potential solution for waste reuse and sustainable use of fertilizers. Granular fertilizers containing primary macronutrients (N, P and K) were formulated using biogas sludge and supplementary chemical compounds before coating with a thin film of glutaraldehyde cross-linked gelatin. The coating films reduced the release rates of N and P effectively. The application of formulated biogas fertilizers resulted in the competitive yield and growth rate of mustard greens over the 45-days cultivation compared to the application of commercial fertilizers. Particularly, the application of coated biogas fertilizers led to less nutrient losses in drainage water while maintained a balanced supply of available nutrients and beneficial bacteria in soils. This study emphasizes the feasibility to reuse biogas sludge for slow-release fertilizers production in improving fertilizer use efficiency and soil fertility.

化肥的过度使用会对环境和作物产量造成负面影响。因此,利用沼气污泥生产缓释肥料是废物再利用和肥料可持续使用的潜在解决方案。利用沼气污泥和辅助化学物质配制了含有主要宏量营养元素(氮、磷和钾)的颗粒肥料,然后涂上一层戊二醛交联明胶薄膜。涂膜有效降低了氮和磷的释放率。与施用商业肥料相比,施用配制的沼气肥料使芥菜在 45 天的栽培过程中产量和生长率都具有竞争力。特别是,施用包膜沼气肥减少了排水中的养分损失,同时保持了土壤中可用养分和有益菌的均衡供应。这项研究强调了将沼气污泥再用于生产缓释肥料以提高肥料利用效率和土壤肥力的可行性。
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引用次数: 0
Advanced valorisation for cork wastewater 软木废水的先进增值技术
Q1 Environmental Science Pub Date : 2024-06-01 DOI: 10.1016/j.biteb.2024.101881
Francisco Javier Yuste-Córdoba, Ana Parralejo Alcobendas, Luis Royano Barroso, Jerónimo González Cortés, Belén Godoy-Cancho

The cork manufacturing process generates cork boiling wastewater, characterized by high COD and phenolic compounds content. The main aim of this study is the advanced valorization of these spills through a green and efficient process with two main stages. Firstly, most of the organic matter was removed by an optimal design of a precipitation with calcium. The resulting depuration rates were in the ranges 37–53 % for COD and 70–87 % for TPC. Secondly, the precipitates generated were subjected to a biodigestion process with production of methane (up to 234 L CH4·kg VS−1) and fertilizers. In conclusion, good recovery of interesting by-products and a good yield of depuration were achieved with the technology proposed, avoiding the use of toxic reagents and demonstrating the potential of concentrate precipitates from CBWs as substrates for biodigestion.

软木塞生产过程中会产生软木塞煮沸废水,其特点是化学需氧量和酚类化合物含量较高。本研究的主要目的是通过一种绿色、高效的工艺(包括两个主要阶段)对这些溢出物进行先进的价值评估。首先,通过钙沉淀的优化设计去除大部分有机物。由此产生的化学需氧量净化率为 37%-53%,总石油碳酸净化率为 70%-87%。其次,产生的沉淀物经过生物发酵过程,产生甲烷(高达 234 L CH4-kg VS-1)和肥料。总之,所提出的技术实现了有趣的副产品的良好回收和良好的净化产量,避免了有毒试剂的使用,并证明了将化学生物武器的浓缩沉淀物作为生物发酵基质的潜力。
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引用次数: 0
Pulp carbon fixation via pyrolysis using ammonium dihydrogen phosphate as an additive 使用磷酸二氢铵作为添加剂通过热解进行纸浆固碳
Q1 Environmental Science Pub Date : 2024-06-01 DOI: 10.1016/j.biteb.2024.101873
Yosuke Muranaka, Taisuke Maki, Hiroyuki Nakagawa, Takashi Mukaeda, Akane Tsuchida, Kazuhiro Mae

In recent years, waste pulp incineration has become a problem due to the large amount of carbon dioxide emissions. The effect of ammonium dihydrogen phosphate (ADP) as an additive agent was investigated by pyrolyzing ADP-supplemented pulp, and the yields of carbon fixation, phosphorus recovery, and nitrogen recovery were calculated. An ADP/pulp ratio of 0.01 mol/g was found to be the optimal amount of ADP addition according to the pyrolysis profile, N fixation, carbon yield, and surface properties. The addition of ADP significantly increased the carbon fixation yield from approximately 30 % to over 80 %. The product activated carbon with ADP possessed surface area of 730 m2/g, which was a 2.4-fold increase compared to 300 m2/g without ADP. In addition, the effect of ADP on intramolecular dehydration of pulp was quantitatively discussed by identifying the amount of water, which was about three water molecules from a 2-unit repetition of pulp structure.

近年来,废纸浆焚烧因排放大量二氧化碳而成为一个问题。通过热解添加 ADP 的纸浆,研究了磷酸二氢铵(ADP)作为添加剂的效果,并计算了碳固定、磷回收和氮回收的产量。根据热解曲线、氮固定率、碳产量和表面特性,ADP/纸浆比为 0.01 mol/g,是最佳的 ADP 添加量。添加 ADP 后,固碳率从 30% 左右大幅提高到 80% 以上。添加了 ADP 的活性炭产品的表面积为 730 平方米/克,比未添加 ADP 的 300 平方米/克增加了 2.4 倍。此外,通过确定水量,定量讨论了 ADP 对纸浆分子内脱水的影响。
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引用次数: 0
Use of residual barley to bioethanol production: Valoration, mycotoxins decontamination and feed production 利用残留大麦生产生物乙醇:估价、霉菌毒素净化和饲料生产
Q1 Environmental Science Pub Date : 2024-06-01 DOI: 10.1016/j.biteb.2024.101878
Gabrielle Fusiger Berwian , Alan Rempel , Jaqueline Garda-Buffon , Luciane Maria Colla

The study evaluates the production of ethanol from waste barley, a potential raw material for biofuel production. The objective of this research is to understand the processes involved in this type of production, including the challenging issue of mycotoxin contamination. These toxins can be metabolized by microorganisms during the fermentation process, making the material suitable for animal feed. The selection of articles was made through Scopus and VOSviewer, which were analyzed to understand the feasibility of producing bioethanol from residual barley. Concluding that residual barley is a viable option for bioethanol production, as it contains significant amounts of starch. Furthermore, it can bring economic and environmental benefits, presenting advantages for the entire production chain, aligning with the principles of a circular economy by utilizing waste that would otherwise be discarded. The research also highlights the importance of adopting sustainable practices in biofuel production.

这项研究评估了利用废大麦生产乙醇的情况,废大麦是生产生物燃料的一种潜在原料。这项研究的目的是了解这类生产所涉及的过程,包括具有挑战性的霉菌毒素污染问题。这些毒素可在发酵过程中被微生物代谢掉,从而使原料适合用作动物饲料。通过 Scopus 和 VOSviewer 对文章进行了筛选和分析,以了解利用残留大麦生产生物乙醇的可行性。结论是,残留大麦是生产生物乙醇的可行选择,因为它含有大量淀粉。此外,它还能带来经济和环境效益,为整个生产链带来优势,通过利用原本会被丢弃的废物,符合循环经济的原则。研究还强调了在生物燃料生产中采用可持续做法的重要性。
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引用次数: 0
Enhanced productivity of nutrient-rich single cell protein from Paradendryphiella arenariae PG1 through valorization of agro-industrial waste: A Green symphony from Waste-to-Protein Approach 通过农用工业废弃物的价值化,提高了从Paradendryphiella arenariae PG1中提取营养丰富的单细胞蛋白的生产率:从废物到蛋白质方法的绿色交响乐
Q1 Environmental Science Pub Date : 2024-06-01 DOI: 10.1016/j.biteb.2024.101884
Priya Rajkumar Sagar , Gopalkumar G. Raol , Darshankumar Prajapati , Dhruv Kapdi , Bhavya Kiri

The rise in global population strains agro-industrial waste management, positioning single-cell protein (SCP) production as a cost-effective solution. Evaluating eight agro-waste residues identified wheat bran with a high solid recovery rate (67.15 %). SCP production increased from 1.29 ± 0.23 gL−1 to 21.05 ± 0.33 gL−1, achieving a microbial conversion efficiency of 90.88 % by Paradendryphiella arenariae (PG1). Nucleic acid content in SCP was reduced by 31 % using NaOH and 36 % via heat shock, significantly enhancing SCP quality, digestibility, and safety. SCP's impact on Caenorhabditis elegans (C. elegans) revealed a maximum lifespan of 18 ± 0.2 days, notable pharyngeal pumping rates (117 ± 2.541 and 67 ± 3.473 min−1 on days 5 and 10), and body bending rates (115.3 ± 0.86 and 44.8 ± 0.28 min−1 on days 5 and 10). This comprehensive approach demonstrates the promise of SCP production in sustainable waste management, resource optimization, and detailed toxicity analysis in C. elegans, broadening the potential applications of SCP in various fields.

全球人口的增长给农用工业废物管理带来了压力,因此单细胞蛋白 (SCP) 生产成为一种具有成本效益的解决方案。对八种农业废料残留物进行评估后发现,麦麸的固体回收率较高(67.15%)。SCP 的产量从 1.29 ± 0.23 gL-1 增加到 21.05 ± 0.33 gL-1,Paradendryphiella arenariae (PG1) 的微生物转化效率达到 90.88%。通过使用 NaOH 和热休克,SCP 中的核酸含量分别降低了 31% 和 36%,从而显著提高了 SCP 的质量、消化率和安全性。SCP 对 elegans(秀丽隐杆线虫)的影响表明,秀丽隐杆线虫的最长寿命为 18 ± 0.2 天,显著的咽部抽吸率(第 5 天和第 10 天分别为 117 ± 2.541 和 67 ± 3.473 min-1)和身体弯曲率(第 5 天和第 10 天分别为 115.3 ± 0.86 和 44.8 ± 0.28 min-1)。这种全面的方法证明了 SCP 生产在可持续废物管理、资源优化和详细的秀丽隐杆线虫毒性分析方面的前景,拓宽了 SCP 在各个领域的潜在应用。
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引用次数: 0
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