Pub Date : 2018-01-01DOI: 10.35841/BIOLOGY-MEDICINE.2.1.15-20
Ibrahim Aa, M. Amr, Kamel Em, Youssef Rfm, Al-Mathour Rs
Objectives: This study was conducted to assess posture balance and fall risk in obese men. Design: A cross sectional study. Methods: Fifty males were represented the sample of the study. We used Biodex balance system for assessing three tests for the subject i.e., Posture stability testing, Limits of stability testing and Fall risk testing. Results: Descriptive statistics of means and standard deviations and correlation analysis using Pearson correlation coefficient revealed no statistical difference between tested parameters with change in BMI. Conclusion: We concluded that BMI alone is not main cause of balance but other factors may have greater role in stability.
{"title":"The relevance of balance assessment to detect balance deficits in obesesubjects: A cross sectional study.","authors":"Ibrahim Aa, M. Amr, Kamel Em, Youssef Rfm, Al-Mathour Rs","doi":"10.35841/BIOLOGY-MEDICINE.2.1.15-20","DOIUrl":"https://doi.org/10.35841/BIOLOGY-MEDICINE.2.1.15-20","url":null,"abstract":"Objectives: This study was conducted to assess posture balance and fall risk in obese men. Design: A cross sectional study. Methods: Fifty males were represented the sample of the study. We used Biodex balance system for assessing three tests for the subject i.e., Posture stability testing, Limits of stability testing and Fall risk testing. Results: Descriptive statistics of means and standard deviations and correlation analysis using Pearson correlation coefficient revealed no statistical difference between tested parameters with change in BMI. Conclusion: We concluded that BMI alone is not main cause of balance but other factors may have greater role in stability.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"2 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70032319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.35841/biology-medicine.2.2.12-25
An, H. Aram
In the post genomic era, identification of a potential miRNA in a computational approach for the significance of a discovery of systemic biomarker to treat diseases is a challenging task to execute. The challenge was addressed by identifying the associate genes from Pubmed, OMIM and DisgeNet and it was followed by identifying the miRNAs and transcription factors of associate target genes from RegNetworks. In the next step, a miRNA based regulatory network was constructed on the basis of association between gene-miR-TFs. Finally, the network was analyzed on the basis of statistical studies and miRNA based compatibility to identify a potential miRNA to be utilized as a biomarker to treat diseases in future. In this article, the computational approach was used for the identification of a miRNA based systemic biomarker in Psoriasis and in future this approach can also be used for other diseases.
在后基因组时代，通过计算方法鉴定潜在的miRNA，以发现系统性生物标志物对治疗疾病的重要性，是一项具有挑战性的任务。通过从Pubmed、OMIM和DisgeNet中鉴定相关基因，随后从RegNetworks中鉴定相关靶基因的mirna和转录因子，解决了这一挑战。下一步，基于基因- mir - tf之间的关联，构建基于miRNA的调控网络。最后，在统计研究和基于miRNA兼容性的基础上，对网络进行分析，以确定未来可能用作治疗疾病的生物标志物的miRNA。在本文中，计算方法被用于鉴定银屑病中基于miRNA的系统生物标志物，未来该方法也可用于其他疾病。
{"title":"A computational approach to identify microRNA (miRNA) based biomarker from the regulation of disease pathology","authors":"An, H. Aram","doi":"10.35841/biology-medicine.2.2.12-25","DOIUrl":"https://doi.org/10.35841/biology-medicine.2.2.12-25","url":null,"abstract":"In the post genomic era, identification of a potential miRNA in a computational approach for the significance of a discovery of systemic biomarker to treat diseases is a challenging task to execute. The challenge was addressed by identifying the associate genes from Pubmed, OMIM and DisgeNet and it was followed by identifying the miRNAs and transcription factors of associate target genes from RegNetworks. In the next step, a miRNA based regulatory network was constructed on the basis of association between gene-miR-TFs. Finally, the network was analyzed on the basis of statistical studies and miRNA based compatibility to identify a potential miRNA to be utilized as a biomarker to treat diseases in future. In this article, the computational approach was used for the identification of a miRNA based systemic biomarker in Psoriasis and in future this approach can also be used for other diseases.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"2 1","pages":"12-25"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70033165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/0974-8369.1000438
A. Kavrakova, B. Georgieva, K. Anachkov, K. Yanev, G. Ivanov, V. Mitev, A. Todorova
Background: The aim of the study is to investigate noninvasive urine specimens in suspected prostate cancer (PCa) patients by a panel of PCA3, TMPRSS2-ERG fusions and GSTP1 promoter hypermethylation. Furthermore, we tested urine specimens for the presence of high-risk Human Papilloma Viruses (HPV) as an inflammatory cofactor in the complicated origin of prostate cancer (PCa). Methods: A total of 50 patients with elevated PSA and/or PCa physiological symptoms were analyzed. RNA and DNA isolation; Reverse transcription; Real-time PCR; DNA sequencing; Bisulfite conversion of DNA; Methylationspecific PCR; Cytological preparations and staining were applied. Results: Molecular fluctuations were registered in most of the patients: neoplastic GSTP1 allele, PCA3 strongly elevated expression or hyperexpression. Only in 4 cases a positive TMPRSS2-ERG status was detected. High-risk HPV types were detected in ~ 35% of our urine specimens, obtained from patients at high risk of PCa based on their molecular profiles. Approximately 96% of detected high-risk HPVs are: 16, 33, 35, 31, distributed in the subgroup with highest oncogenic potential. The estimated frequency of high-risk HPV types in control male samples with urothelial infection is significantly lower (11%). The pathological examination on cytological slides from high-risk HPV positive urine specimens showed inflammation; variable adaptations of cellular growth and differentiation and partially viral cytopathic effect. In a proportion of patients with molecular PCa disturbed profile precancerous conditions (increased primitive cells with disturbed maturation; enlarged hyperchromatic nucleus and condensed chromatin) were found. Conclusion: Our molecular PCa findings, were confirmed on the cellular level with cytological findings of high grade alterations: coarse distributed chromatin texture with nuclear membrane irregularity and thickening; high N:C Ratio; prominence of nucleoli and irregularity in shape thereof; identical monotonous nucleoli present in all cells in a group (i.e., "Clonal" pattern); Tumor diathesis. The present study concerns novel data for Bulgarian PCa patients.
{"title":"Investigation of Noninvasive Urine Specimens with Molecular Fluctuations for a Presence of High-Risk Human Papilloma Viruses as an Inflammatory Cofactor in the Prostate Cancer","authors":"A. Kavrakova, B. Georgieva, K. Anachkov, K. Yanev, G. Ivanov, V. Mitev, A. Todorova","doi":"10.4172/0974-8369.1000438","DOIUrl":"https://doi.org/10.4172/0974-8369.1000438","url":null,"abstract":"Background: The aim of the study is to investigate noninvasive urine specimens in suspected prostate cancer (PCa) patients by a panel of PCA3, TMPRSS2-ERG fusions and GSTP1 promoter hypermethylation. Furthermore, we tested urine specimens for the presence of high-risk Human Papilloma Viruses (HPV) as an inflammatory cofactor in the complicated origin of prostate cancer (PCa). Methods: A total of 50 patients with elevated PSA and/or PCa physiological symptoms were analyzed. RNA and DNA isolation; Reverse transcription; Real-time PCR; DNA sequencing; Bisulfite conversion of DNA; Methylationspecific PCR; Cytological preparations and staining were applied. Results: Molecular fluctuations were registered in most of the patients: neoplastic GSTP1 allele, PCA3 strongly elevated expression or hyperexpression. Only in 4 cases a positive TMPRSS2-ERG status was detected. High-risk HPV types were detected in ~ 35% of our urine specimens, obtained from patients at high risk of PCa based on their molecular profiles. Approximately 96% of detected high-risk HPVs are: 16, 33, 35, 31, distributed in the subgroup with highest oncogenic potential. The estimated frequency of high-risk HPV types in control male samples with urothelial infection is significantly lower (11%). The pathological examination on cytological slides from high-risk HPV positive urine specimens showed inflammation; variable adaptations of cellular growth and differentiation and partially viral cytopathic effect. In a proportion of patients with molecular PCa disturbed profile precancerous conditions (increased primitive cells with disturbed maturation; enlarged hyperchromatic nucleus and condensed chromatin) were found. Conclusion: Our molecular PCa findings, were confirmed on the cellular level with cytological findings of high grade alterations: coarse distributed chromatin texture with nuclear membrane irregularity and thickening; high N:C Ratio; prominence of nucleoli and irregularity in shape thereof; identical monotonous nucleoli present in all cells in a group (i.e., \"Clonal\" pattern); Tumor diathesis. The present study concerns novel data for Bulgarian PCa patients.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"10 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/0974-8369.1000438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70929846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/0974-8369.1000448
M. Takenokuchi, K. Kadoyama, Daisuke Yoshida, Shigeki Takaki, R. Yamamoto, K. Saigo, T. Taniguchi
Introduction: Collagen exists in various connective tissues and helps to provide them mechanical strength and elasticity. The main component of the tendon is properly arranged collagen fibers withstanding very strong forces. The compactly packed collagen fine fibers inside the bone and cartilage increase their elasticity. Collagen also helps skin elasticity and strength. Hyaluronic acid widely exists in extracellular matrix, such as joints and skin. It plays an important role in maintaining cartilage function by making ultra-macromolecular complexes with aggrecan or protein. These collagen and hyaluronic acid decrease with age. Therefore oral supplementation or cosmetics containing them are developed day by day and catch interests of people trackleing anti-aging. The objective of this study was to evaluate and compare the absorbability of macromolecular substances in the oral mucosa and in the skin. Methods: As animal experiments cannot be performed for the evaluation of cosmetics, we used a threedimensional oral mucosa culture model and a three-dimensional epidermis culture model. The absorbability of macromolecular substances with molecular weights from 4,000 to 2,000,000 Da was compared between the oral mucosa model and the skin model. Results: In the skin model, the amount permeated were quite low on all molecular weight. In the oral mucosa model, it absorbed much more compared with that of skin model, though the amount decreased according to the molecular weight. Conclusion: This result was considered due to the absence of the stratum corneum in the oral mucosa. High absorbability through oral mucosa shows the possibility of a new absorption pathway of macromolecular substances.
{"title":"Evaluation of Absorbability of Macromolecular Substances in the Oral Mucosa and Skin using a Three-Dimensional Tissue Culture Model","authors":"M. Takenokuchi, K. Kadoyama, Daisuke Yoshida, Shigeki Takaki, R. Yamamoto, K. Saigo, T. Taniguchi","doi":"10.4172/0974-8369.1000448","DOIUrl":"https://doi.org/10.4172/0974-8369.1000448","url":null,"abstract":"Introduction: Collagen exists in various connective tissues and helps to provide them mechanical strength and elasticity. The main component of the tendon is properly arranged collagen fibers withstanding very strong forces. The compactly packed collagen fine fibers inside the bone and cartilage increase their elasticity. Collagen also helps skin elasticity and strength. Hyaluronic acid widely exists in extracellular matrix, such as joints and skin. It plays an important role in maintaining cartilage function by making ultra-macromolecular complexes with aggrecan or protein. These collagen and hyaluronic acid decrease with age. Therefore oral supplementation or cosmetics containing them are developed day by day and catch interests of people trackleing anti-aging. The objective of this study was to evaluate and compare the absorbability of macromolecular substances in the oral mucosa and in the skin. Methods: As animal experiments cannot be performed for the evaluation of cosmetics, we used a threedimensional oral mucosa culture model and a three-dimensional epidermis culture model. The absorbability of macromolecular substances with molecular weights from 4,000 to 2,000,000 Da was compared between the oral mucosa model and the skin model. Results: In the skin model, the amount permeated were quite low on all molecular weight. In the oral mucosa model, it absorbed much more compared with that of skin model, though the amount decreased according to the molecular weight. Conclusion: This result was considered due to the absence of the stratum corneum in the oral mucosa. High absorbability through oral mucosa shows the possibility of a new absorption pathway of macromolecular substances.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"10 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70930080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/0974-8369.1000442
Rekha M. Ravindran, S. Kumar, J. Rajkumar, Sujata Roy, S. Sathiya, C. Babu, M. J. Equbal
Aims and objectives: The current study characterized the morphology of Ambrex formulation by Scanning Electron Microscopy and assessed its cardioprotective activity against Isoproterenol (ISPH)-induced myocardial necrosis in rats by biochemical and histopathological evaluations, and also attempted to predict the prospective protein-targets of Ambrex and the signaling pathway that mediates this activity through molecular docking approach. Materials and methods: Sprague–Dawley male rats (4 groups, 6 rats per group) chosen for the current study were acclimatized to the laboratory conditions for 7 days prior to actual treatment; they were pretreated with Ambrex (40 mg/kg b.wt/day, p.o) everday for 21 days and then intoxicated with ISPH (85 mg/kg b.wt, s.c) on day-20 and 21 to experimentally induce myocardial necrosis. The extent of ISPH-induced myocardial necrosis was quantified in terms of the serum levels of two cardiac biomarkers: creatine kinase-MB and lactate dehydrogenase. The extent of ISPH-induced oxidative stress was quantified in terms of the tissue levels of five oxidative stress biomarkers: superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase and lipid peroxidation. Results and discussion: The Scanning Electron Microscopy image of Ambrex formulation showed the formation of nanoparticles with thickness of 65 nm, making Ambrex a unique metal-deficient Siddha-medicine based polyherbal nano-formulation characterized and evaluated in India. Pretreatment with Ambrex attenuated the extent of ISPH-induced oxidative stress, lipid peroxidation and generation of reactive oxygen species as reflected by biochemical evaluations, and also ameliorated the degree of ISPH-induced myocardial necrosis and membrane damage as reflected by histopathological evaluations. The results of molecular docking revealed that Withaferin-A and Methyl Commate-A (the key metabolites of Withania somnifera and Ambrex respectively) inhibit Protein KinaseC Beta, and renders Ambrex its cardioprotective activity by maintaining the intracellular antioxidant homeostasis and myocardial membrane architecture.
{"title":"Inhibition of PKCβ Mediates Cardioprotective Activity of Ambrex against Isoproterenol-Induced Myocardial Necrosis: in vivo and in silico Studies","authors":"Rekha M. Ravindran, S. Kumar, J. Rajkumar, Sujata Roy, S. Sathiya, C. Babu, M. J. Equbal","doi":"10.4172/0974-8369.1000442","DOIUrl":"https://doi.org/10.4172/0974-8369.1000442","url":null,"abstract":"Aims and objectives: The current study characterized the morphology of Ambrex formulation by Scanning Electron Microscopy and assessed its cardioprotective activity against Isoproterenol (ISPH)-induced myocardial necrosis in rats by biochemical and histopathological evaluations, and also attempted to predict the prospective protein-targets of Ambrex and the signaling pathway that mediates this activity through molecular docking approach. Materials and methods: Sprague–Dawley male rats (4 groups, 6 rats per group) chosen for the current study were acclimatized to the laboratory conditions for 7 days prior to actual treatment; they were pretreated with Ambrex (40 mg/kg b.wt/day, p.o) everday for 21 days and then intoxicated with ISPH (85 mg/kg b.wt, s.c) on day-20 and 21 to experimentally induce myocardial necrosis. The extent of ISPH-induced myocardial necrosis was quantified in terms of the serum levels of two cardiac biomarkers: creatine kinase-MB and lactate dehydrogenase. The extent of ISPH-induced oxidative stress was quantified in terms of the tissue levels of five oxidative stress biomarkers: superoxide dismutase, catalase, reduced glutathione, glutathione peroxidase and lipid peroxidation. Results and discussion: The Scanning Electron Microscopy image of Ambrex formulation showed the formation of nanoparticles with thickness of 65 nm, making Ambrex a unique metal-deficient Siddha-medicine based polyherbal nano-formulation characterized and evaluated in India. Pretreatment with Ambrex attenuated the extent of ISPH-induced oxidative stress, lipid peroxidation and generation of reactive oxygen species as reflected by biochemical evaluations, and also ameliorated the degree of ISPH-induced myocardial necrosis and membrane damage as reflected by histopathological evaluations. The results of molecular docking revealed that Withaferin-A and Methyl Commate-A (the key metabolites of Withania somnifera and Ambrex respectively) inhibit Protein KinaseC Beta, and renders Ambrex its cardioprotective activity by maintaining the intracellular antioxidant homeostasis and myocardial membrane architecture.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"10 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70929698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-01-01DOI: 10.4172/0974-8369.1000450
M. Ciampolini
List of Abbreviations: BG: Blood Glucose; an index of energy availability in blood for the whole body; Initial Hunger Meal Pattern (IHMP): Energy intake is adjusted to three arousals of initial hunger per day; Initial Hunger: This consists of gastric pangs or physical weakness associated with low blood glucose that arise after meal suspension for either few minutes or hours; This arousal misses in diabetic patients; MBG: mean blood glucose: mean of BG measurements before three main meals in a week.
{"title":"The Biological Role of Intestine: Overall Inflammation","authors":"M. Ciampolini","doi":"10.4172/0974-8369.1000450","DOIUrl":"https://doi.org/10.4172/0974-8369.1000450","url":null,"abstract":"List of Abbreviations: BG: Blood Glucose; an index of energy availability in blood for the whole body; Initial Hunger Meal Pattern (IHMP): Energy intake is adjusted to three arousals of initial hunger per day; Initial Hunger: This consists of gastric pangs or physical weakness associated with low blood glucose that arise after meal suspension for either few minutes or hours; This arousal misses in diabetic patients; MBG: mean blood glucose: mean of BG measurements before three main meals in a week.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70930210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-11-15DOI: 10.4172/0974-8369.1000424
Huma Butt
Leukemia is a third fatal disease in developing countries. According to Punjab cancer registry 2014 report, leukemia incidence in Pakistan is 18.8%. Many treatments are available for leukemia now days but still there is a mortality rate measured. BCL2 controls cell apoptosis but when overexpressed due to the presence of promoter region single nucleotide polymorphism 938C>A, it causes anticancer drug resistance and represses apoptosis of malfunctioned cells resulting in immature leucocytes. The aim of the present study was genotyping of promoter region SNP in acute lymphoblastic leukemia patients of Pakistan and screening of naturally occurring phytochemical to identify BCL2 active site targeting compounds to control its expression. Study was performed on 104 cases taking similar control size showing allelic frequency C=0.466 and A=0.534 in cases, indicating an association with the disease by having chi-square p-value 0.0032. Screening of 90 phytochemicals was performed out of which one compound was selected as a lead compound based upon drug likeliness properties, binding with protein, and natural existence that may cause the least harm to human body and greater effect on leukemia cells. The information provided by this research can be used to make new-targeted drugs to control leukemia by inhibiting the mutated proteins.
{"title":"Polymorphisms of BCL2 Gene in Acute Lymphoblastic Leukemia Patients in Pakistan and Screening of Phytochemicals to Overcome its Expression","authors":"Huma Butt","doi":"10.4172/0974-8369.1000424","DOIUrl":"https://doi.org/10.4172/0974-8369.1000424","url":null,"abstract":"Leukemia is a third fatal disease in developing countries. According to Punjab cancer registry 2014 report, leukemia incidence in Pakistan is 18.8%. Many treatments are available for leukemia now days but still there is a mortality rate measured. BCL2 controls cell apoptosis but when overexpressed due to the presence of promoter region single nucleotide polymorphism 938C>A, it causes anticancer drug resistance and represses apoptosis of malfunctioned cells resulting in immature leucocytes. The aim of the present study was genotyping of promoter region SNP in acute lymphoblastic leukemia patients of Pakistan and screening of naturally occurring phytochemical to identify BCL2 active site targeting compounds to control its expression. Study was performed on 104 cases taking similar control size showing allelic frequency C=0.466 and A=0.534 in cases, indicating an association with the disease by having chi-square p-value 0.0032. Screening of 90 phytochemicals was performed out of which one compound was selected as a lead compound based upon drug likeliness properties, binding with protein, and natural existence that may cause the least harm to human body and greater effect on leukemia cells. The information provided by this research can be used to make new-targeted drugs to control leukemia by inhibiting the mutated proteins.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"10 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2017-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41740964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-10-18DOI: 10.4172/0974-8369.1000416
Bassam Aboul-Nasr, Abdel-Naser A. Zohri, M. Adam, E. Amer
Aspergillosis remains one of the most challenging areas of illness. It has recently emerged as a world-wide health care problem, owing to extensive use of broad spectrum antibiotics, immunosuppressive agents and increasing population of terminally ill and debilitated patients. The ultimate aim of this investigation is to minimize morbidity and mortality of mechanically ventilated aspergillosis suspected patients, who have been notoriously difficult to be diagnosed and treated, by developing an accurate and easy diagnostic tool comparable to other conventional methods. Two hundred twenty seven suspected aspergillosis patients were screened for fungal infection using different conventional tools to evaluate different respiratory specimens sputum, bronchoalveolar lavage, plural fluid, blood, biopsy and ELISA technique for galactomannan and (1-3)-β-D-glucan antigens in serum and bronchoalveolar lavage. Four Aspergillus species (A. niger, A. fumigatus, A. flavus and A. terreus) were isolated and identified morphologically and genetically using the conventional diagnostic tools from suspected patients in all positive cultures. The results of the exhalation air of twenty three mechanically ventilated aspergillosis suspected patients came in accordance with those of the conventional methods used. Thus, exhalation air culture is an easily performed bedside tool for diagnosis of aspergillosis in ventilated patients.
曲霉病仍然是最具挑战性的疾病领域之一。由于广泛使用广谱抗生素、免疫抑制剂以及越来越多的绝症患者和衰弱患者，它最近已成为一个世界性的保健问题。本研究的最终目的是通过开发一种与其他常规方法相媲美的准确、简便的诊断工具，最大限度地减少机械通气曲霉病疑似患者的发病率和死亡率，这些患者一直是出了名的难以诊断和治疗。对227例疑似曲霉病患者进行真菌感染筛查，采用不同常规工具评估不同呼吸道标本(痰、支气管肺泡灌洗液、多元液、血液、活检)及ELISA法检测血清和支气管肺泡灌洗液中半乳甘露聚糖和(1-3)-β- d -葡聚糖抗原。从所有阳性培养的疑似患者中分离出4种曲霉(黑曲霉、烟曲霉、黄曲霉和地曲霉)，并利用常规诊断工具进行形态学和遗传学鉴定。对23例机械通气曲霉病疑似患者呼气结果与常规方法一致。因此，呼气空气培养是一种易于进行的床边工具，诊断曲霉病的通气患者。
{"title":"Exhalation Air Fungal Culture of Mechanically Ventilated Aspergillosis Suspected Patients in Concordance with Other Conventional Diagnostic Techniques","authors":"Bassam Aboul-Nasr, Abdel-Naser A. Zohri, M. Adam, E. Amer","doi":"10.4172/0974-8369.1000416","DOIUrl":"https://doi.org/10.4172/0974-8369.1000416","url":null,"abstract":"Aspergillosis remains one of the most challenging areas of illness. It has recently emerged as a world-wide health care problem, owing to extensive use of broad spectrum antibiotics, immunosuppressive agents and increasing population of terminally ill and debilitated patients. The ultimate aim of this investigation is to minimize morbidity and mortality of mechanically ventilated aspergillosis suspected patients, who have been notoriously difficult to be diagnosed and treated, by developing an accurate and easy diagnostic tool comparable to other conventional methods. Two hundred twenty seven suspected aspergillosis patients were screened for fungal infection using different conventional tools to evaluate different respiratory specimens sputum, bronchoalveolar lavage, plural fluid, blood, biopsy and ELISA technique for galactomannan and (1-3)-β-D-glucan antigens in serum and bronchoalveolar lavage. Four Aspergillus species (A. niger, A. fumigatus, A. flavus and A. terreus) were isolated and identified morphologically and genetically using the conventional diagnostic tools from suspected patients in all positive cultures. The results of the exhalation air of twenty three mechanically ventilated aspergillosis suspected patients came in accordance with those of the conventional methods used. Thus, exhalation air culture is an easily performed bedside tool for diagnosis of aspergillosis in ventilated patients.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":" ","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44047062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-21DOI: 10.14302/ISSN.2574-4526.JDDD-17-1498
Tilley Jc, J. Dubuisson
We report a rare case of association between a mature cystic teratoma (MCT) and an endometrioma in the ovary. This entity is extremely rare and its diagnostic is a challenge clinically and radiologically. To our knowledge we report the fourth case of the coexistence of a non-neoplastic endometrioma and a benign neoplastic mature cystic teratoma in the left ovary.
{"title":"Coexistence of Mature Cystic Teratoma and Endometrioma in an Ovarian Cyst","authors":"Tilley Jc, J. Dubuisson","doi":"10.14302/ISSN.2574-4526.JDDD-17-1498","DOIUrl":"https://doi.org/10.14302/ISSN.2574-4526.JDDD-17-1498","url":null,"abstract":"We report a rare case of association between a mature cystic teratoma (MCT) and an endometrioma in the ovary. This entity is extremely rare and its diagnostic is a challenge clinically and radiologically. To our knowledge we report the fourth case of the coexistence of a non-neoplastic endometrioma and a benign neoplastic mature cystic teratoma in the left ovary.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"1 1","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2017-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46248766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-27DOI: 10.4172/0974-8369.1000405
N. Vilalta, I. Tirado, J. Mateo, L. Romero, Marina Carrasco, J. Souto, J. Fontcuberta
Miyawaky et al. have identified recently a new prothrombin gene mutation c.1787G>T in a Japanese family with hereditary thrombophilia [4]. This mutation is located in the last exon of the prothrombin gene resulting in Arg596Leu replacement. Djordjevic et al. [5] also have described another mutation at the same prothrombin gene position (c. 1787G>A prothrombin Belgrade) that results in aminoacid change (Arg596Gln). The mutant prothrombins were shown to have reduced activity in clotting assays and the produced thrombin was markedly resistant to inhibition by antithrombin.
{"title":"The c.1787G>T and c.1787G>A Mutations are not Found in the Prothrombin Gene in a Spanish Population","authors":"N. Vilalta, I. Tirado, J. Mateo, L. Romero, Marina Carrasco, J. Souto, J. Fontcuberta","doi":"10.4172/0974-8369.1000405","DOIUrl":"https://doi.org/10.4172/0974-8369.1000405","url":null,"abstract":"Miyawaky et al. have identified recently a new prothrombin gene mutation c.1787G>T in a Japanese family with hereditary thrombophilia [4]. This mutation is located in the last exon of the prothrombin gene resulting in Arg596Leu replacement. Djordjevic et al. [5] also have described another mutation at the same prothrombin gene position (c. 1787G>A prothrombin Belgrade) that results in aminoacid change (Arg596Gln). The mutant prothrombins were shown to have reduced activity in clotting assays and the produced thrombin was markedly resistant to inhibition by antithrombin.","PeriodicalId":90454,"journal":{"name":"Biology and medicine (Aligarh)","volume":"2017 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/0974-8369.1000405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42804136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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