Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.22
O. Nechytailo, O. Kolotylo
Relevance. The global coronavirus pandemic has made significant adjustments to the higher education system, inducing the search for optimal teaching methods that will optimize the educational process for all its applicants, and will enable to perform an adequate assessment of the level of knowledge of students.Purpose of work. Determine the best methods of online teaching material for students, depending on their characteristics of perception of information, as well as provide an effective way to control knowledge.Material and methods. During the quarantine period and online training, the Google Meet platform was used to conduct online workshops and seminars. Depending on whether the information channel of information perception prevailed among the majority of students in the group, an individual approach to presenting information with different types of clarity was used. Evaluation of the effectiveness of various methods of presenting the material was carried out through intermediate and current control of knowledge, as well as oral questioning of students at the end of the semester.Results. The highest productivity of information perception was found when combining different teaching methods during one lesson, when students take turns using a sound, visual analyzer, and also holding pre-printed materials in their hands. The use of short scenarios of simulation medicine, when there is an opportunity to improve communicative competence, significantly increases the activity of students during the lesson, manifests itself in the number of "raised hands" and comments in the chat. The ingenuity and perseverance of the teacher makes it possible to objectively assess the knowledge of students, despite the obvious difficulties, online learning brings. Conclusion. During quarantine and distance learning, the most effective are the methods of combined presentation of the material, when students have the opportunity to use all channels of information perception.
{"title":"Changes in the modern university: challenges and development trends","authors":"O. Nechytailo, O. Kolotylo","doi":"10.24061/2413-0737.xxv.4.100.2021.22","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.22","url":null,"abstract":"Relevance. The global coronavirus pandemic has made significant adjustments to the higher education system, inducing the search for optimal teaching methods that will optimize the educational process for all its applicants, and will enable to perform an adequate assessment of the level of knowledge of students.Purpose of work. Determine the best methods of online teaching material for students, depending on their characteristics of perception of information, as well as provide an effective way to control knowledge.Material and methods. During the quarantine period and online training, the Google Meet platform was used to conduct online workshops and seminars. Depending on whether the information channel of information perception prevailed among the majority of students in the group, an individual approach to presenting information with different types of clarity was used. Evaluation of the effectiveness of various methods of presenting the material was carried out through intermediate and current control of knowledge, as well as oral questioning of students at the end of the semester.Results. The highest productivity of information perception was found when combining different teaching methods during one lesson, when students take turns using a sound, visual analyzer, and also holding pre-printed materials in their hands. The use of short scenarios of simulation medicine, when there is an opportunity to improve communicative competence, significantly increases the activity of students during the lesson, manifests itself in the number of \"raised hands\" and comments in the chat. The ingenuity and perseverance of the teacher makes it possible to objectively assess the knowledge of students, despite the obvious difficulties, online learning brings. Conclusion. During quarantine and distance learning, the most effective are the methods of combined presentation of the material, when students have the opportunity to use all channels of information perception.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75873473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.9
K. Moiseenko
Background. Ischemic atherothrombotic stroke (IATS) – multifactorial disease caused by a combination of genetic and environmental factors. Despite numerous studies of the pathogenetic component of ischemic stroke, there are no preclinical biomarkers that could support the study of molecular and physiological processes that lead to stroke. That's why a strategy for studying the pathogenesis of stroke is to start by identifying the genes associated with stroke. In particular, with ischemic stroke, an abnormal expression of MALAT1 plays an important role in such processes as angiogenesis, apoptosis, and inflammation. However, the study of the connection of the SNP variant of the MALAT1 with an IATS is few and not carried out in the Ukrainian population.The aim of this study was to investigate the possible association of rs4102217-polymorphism of the MALAT1 gene with ischemic stroke in individuals with normal and high blood pressure. Materials and methods. Venous blood of 200 patients with IATS was used for the study; the control group consisted of 234 practically healthy donors. To determine the SNP of rs4102217 MALAT1 was used polymerase chain reaction (Real-Time PCR). SPSS program (version 17.0, IBM, USA) was used for most statistical analyses. Results. It was found that the ratio of GG, GC and CC genotypes among the persons of the control group that had an increased AP - 84.9%, 13.7% and 1.4% respectively, and in the subgroup of persons from the main group with high blood pressure - 62,7%, 30.0%, 7.3% respectively. By applying χ2 – Pearson criterion was established a statistically significant difference in the distribution of genotypes between the comparative groups (χ2 - 12,015; P - 0,002). The results of logistic regression analysis showed statistically significant associations of IATS polymorphism in three models. So, the risk of development of IATS increases 2,97 times for patients with arterial hypertension, who are carriers of the C-allele (ORadj = 2,97; 95% CI = 1,286-6,785; Padj = 0,011 - for a dominant model). Conclusions. There is a link between the rs4102217-polymorphism of the MALAT1 gene and the development of IATI in people with normal and high blood pressure.
{"title":"Analysis of relationship between rs4102217-MALAT1 gene polymorphism with ischemic atherothrobotic stroke in persons with normal and high blood pressure","authors":"K. Moiseenko","doi":"10.24061/2413-0737.xxv.4.100.2021.9","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.9","url":null,"abstract":"Background. Ischemic atherothrombotic stroke (IATS) – multifactorial disease caused by a combination of genetic and environmental factors. Despite numerous studies of the pathogenetic component of ischemic stroke, there are no preclinical biomarkers that could support the study of molecular and physiological processes that lead to stroke. That's why a strategy for studying the pathogenesis of stroke is to start by identifying the genes associated with stroke. In particular, with ischemic stroke, an abnormal expression of MALAT1 plays an important role in such processes as angiogenesis, apoptosis, and inflammation. However, the study of the connection of the SNP variant of the MALAT1 with an IATS is few and not carried out in the Ukrainian population.The aim of this study was to investigate the possible association of rs4102217-polymorphism of the MALAT1 gene with ischemic stroke in individuals with normal and high blood pressure. Materials and methods. Venous blood of 200 patients with IATS was used for the study; the control group consisted of 234 practically healthy donors. To determine the SNP of rs4102217 MALAT1 was used polymerase chain reaction (Real-Time PCR). SPSS program (version 17.0, IBM, USA) was used for most statistical analyses. Results. It was found that the ratio of GG, GC and CC genotypes among the persons of the control group that had an increased AP - 84.9%, 13.7% and 1.4% respectively, and in the subgroup of persons from the main group with high blood pressure - 62,7%, 30.0%, 7.3% respectively. By applying χ2 – Pearson criterion was established a statistically significant difference in the distribution of genotypes between the comparative groups (χ2 - 12,015; P - 0,002). The results of logistic regression analysis showed statistically significant associations of IATS polymorphism in three models. So, the risk of development of IATS increases 2,97 times for patients with arterial hypertension, who are carriers of the C-allele (ORadj = 2,97; 95% CI = 1,286-6,785; Padj = 0,011 - for a dominant model). Conclusions. There is a link between the rs4102217-polymorphism of the MALAT1 gene and the development of IATI in people with normal and high blood pressure.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89844711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.7
I. Malyshevskyi
The aim – to establish the clinical and pathogenetic significance of overweight and comorbidity in patients with malignant neoplasms (MN) of the abdominal cavity. Material and methods. The study was performed on the basis of the Chernivtsi Regional Clinical Oncology Centre and the Oncology Clinic of Bukovinian State Medical University and covered observation materials of 50 patients with intra-abdominal neoplasms. Among them, 35 individualsforming the main group were obese (body mass index ≥30), the rest formed the comparison group. Comorbidity index, risk complication ratio, confidence intervals, Fisher (P) and χ2 coefficients were calculated according to Yates and Pearson. Results. In the comparison group, comorbidities were detected in only 6 patients (40.0%), in the main group, comorbidities were detected in all patients. The value of the comorbidity index in the main group was 11.23±0.98 against 6.37±0.72 (P=0.005). The risk of complications increases significantly in the presence of obesity (OR+ 2.49, min-1.29, max-4.80). The term of hospitalization increases slightly (22.36±3.14 in the main group, against 16.73±2.50 in the comparison group, P=0.18). Conclusions. In patients with malignant neoplasms of the abdominal cavity, the risk of postoperative complications is 1.29-6.96 times higher than in the absence of obesity. The presence of obesity is a significant factor in aggravating the condition, prolongs the period of hospitalization of patients with malignant neoplasms, creates additional challenges for the oncologist.
{"title":"Overweight and comorbidity in patients with malignant tumors of the abdominal organs","authors":"I. Malyshevskyi","doi":"10.24061/2413-0737.xxv.4.100.2021.7","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.7","url":null,"abstract":"The aim – to establish the clinical and pathogenetic significance of overweight and comorbidity in patients with malignant neoplasms (MN) of the abdominal cavity. Material and methods. The study was performed on the basis of the Chernivtsi Regional Clinical Oncology Centre and the Oncology Clinic of Bukovinian State Medical University and covered observation materials of 50 patients with intra-abdominal neoplasms. Among them, 35 individualsforming the main group were obese (body mass index ≥30), the rest formed the comparison group. Comorbidity index, risk complication ratio, confidence intervals, Fisher (P) and χ2 coefficients were calculated according to Yates and Pearson. Results. In the comparison group, comorbidities were detected in only 6 patients (40.0%), in the main group, comorbidities were detected in all patients. The value of the comorbidity index in the main group was 11.23±0.98 against 6.37±0.72 (P=0.005). The risk of complications increases significantly in the presence of obesity (OR+ 2.49, min-1.29, max-4.80). The term of hospitalization increases slightly (22.36±3.14 in the main group, against 16.73±2.50 in the comparison group, P=0.18). Conclusions. In patients with malignant neoplasms of the abdominal cavity, the risk of postoperative complications is 1.29-6.96 times higher than in the absence of obesity. The presence of obesity is a significant factor in aggravating the condition, prolongs the period of hospitalization of patients with malignant neoplasms, creates additional challenges for the oncologist.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90152943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.13
O. Radchenko, O. Komarytsia, L. Strilchuk, O. Zimba
Systemic vasculitides are often accompanied by the liver affection, but the data on hemorrhagic vasculitis is limited. The aim of this paper was to determine the structural and functional condition of liver and gallbladder (GB) in patients with hemorrhagic vasculitis. Materials and methods. We retrospectively analyzed data of 75 patients (57% females and 43% males, mean age 47.7±4.3 years) and estimated the structural and functional condition of their liver and GB (total bilirubin, transaminases, gamma-glutamyltranspeptydase (GGTP), alkaline phosphatase). The digital data was statistically processed and represented as M±m. Correlations were estimated according to Pearson (r). Data was considered significant if р<0.05. Results. It was revealed that sonographic signs of liver affection were present in 73.9% of patients with hemorrhagic vasculitis; sonographic signs of GB affection – in 61.3%. It is worth saying that these changes were mentioned in the diagnosis of 3.9% of patients only, which is a sign of insufficient attention of doctors to these changes. The mean values of routine laboratory markers of liver affection were in the normal range. We revealed hypobilirubinemia in 46.5% of patients, which can testify the frequent presence of oxidative stress. In our patients we also noticed hyperbilirubinemia (4.2%) and the increase of aspartate aminotransferase (2.7%), alanine aminotransferase (4.0%), de Ritis index (42.7%), GGTP (36.4%) and alkaline phosphatase (53.3%). Conclusions. Bilirubin level directly correlated with hemoglobin; aspartate and alanine aminotransferases - inversely with total cholesterol; de Ritis index – with alpha-globulines, cholestasis markers, peripheral blood erythrocytes and monocytes; GGTP – with erythrocytes and erythrocyte sedimentation rate.
{"title":"Condition of hepatobiliary zone in patients with hemorrhagic vasculitis","authors":"O. Radchenko, O. Komarytsia, L. Strilchuk, O. Zimba","doi":"10.24061/2413-0737.xxv.4.100.2021.13","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.13","url":null,"abstract":"Systemic vasculitides are often accompanied by the liver affection, but the data on hemorrhagic vasculitis is limited. The aim of this paper was to determine the structural and functional condition of liver and gallbladder (GB) in patients with hemorrhagic vasculitis. Materials and methods. We retrospectively analyzed data of 75 patients (57% females and 43% males, mean age 47.7±4.3 years) and estimated the structural and functional condition of their liver and GB (total bilirubin, transaminases, gamma-glutamyltranspeptydase (GGTP), alkaline phosphatase). The digital data was statistically processed and represented as M±m. Correlations were estimated according to Pearson (r). Data was considered significant if р<0.05. Results. It was revealed that sonographic signs of liver affection were present in 73.9% of patients with hemorrhagic vasculitis; sonographic signs of GB affection – in 61.3%. It is worth saying that these changes were mentioned in the diagnosis of 3.9% of patients only, which is a sign of insufficient attention of doctors to these changes. The mean values of routine laboratory markers of liver affection were in the normal range. We revealed hypobilirubinemia in 46.5% of patients, which can testify the frequent presence of oxidative stress. In our patients we also noticed hyperbilirubinemia (4.2%) and the increase of aspartate aminotransferase (2.7%), alanine aminotransferase (4.0%), de Ritis index (42.7%), GGTP (36.4%) and alkaline phosphatase (53.3%). Conclusions. Bilirubin level directly correlated with hemoglobin; aspartate and alanine aminotransferases - inversely with total cholesterol; de Ritis index – with alpha-globulines, cholestasis markers, peripheral blood erythrocytes and monocytes; GGTP – with erythrocytes and erythrocyte sedimentation rate.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80041215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.8
O. Mykhaloiko
High platelet reactivity in patients with ischemic noncardioembolic stroke is associated with an increased risk of recurrent ischemic events. However, their determinants are still poorly understood. The aim of our study was to examine changes in mean platelet volume in patients with ischemic stroke in the early recovery period depending on comorbidity.Material and methods. Clinical and laboratory analysis of 90 patients with acute noncardioembolic stroke of the early recovery period. Among those surveyed were 49 men and 41 women aged (60.42 ± 6.8) years. Platelet count (PLT) and mean platelet volume (MPV) were determined on an Abacus 30 hematology analyzer.Results. Significantly more often, the highest mean platelet counts were observed in patients with atherothrombotic subtype of ischemic stroke with greater neurological deficit. Patients with the highest MPV rates had a higher prevalence of diabetes, an elevated body mass index, and a degree of cerebral atherosclerosis compared with patients with lower MPV levels.Conclusion. The increase in MPV in patients with ischemic stroke is observed in parallel with the increase in the influence of known factors in the development of cardiovascular diseases that cause the progression of vascular endothelial damage and atherothrombosis and indicates an increased risk of recurrent stroke.
{"title":"Variability of mean platelet volume in patients with ischemic stroke in the early recovery period depending on comorbidity","authors":"O. Mykhaloiko","doi":"10.24061/2413-0737.xxv.4.100.2021.8","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.8","url":null,"abstract":"High platelet reactivity in patients with ischemic noncardioembolic stroke is associated with an increased risk of recurrent ischemic events. However, their determinants are still poorly understood. The aim of our study was to examine changes in mean platelet volume in patients with ischemic stroke in the early recovery period depending on comorbidity.Material and methods. Clinical and laboratory analysis of 90 patients with acute noncardioembolic stroke of the early recovery period. Among those surveyed were 49 men and 41 women aged (60.42 ± 6.8) years. Platelet count (PLT) and mean platelet volume (MPV) were determined on an Abacus 30 hematology analyzer.Results. Significantly more often, the highest mean platelet counts were observed in patients with atherothrombotic subtype of ischemic stroke with greater neurological deficit. Patients with the highest MPV rates had a higher prevalence of diabetes, an elevated body mass index, and a degree of cerebral atherosclerosis compared with patients with lower MPV levels.Conclusion. The increase in MPV in patients with ischemic stroke is observed in parallel with the increase in the influence of known factors in the development of cardiovascular diseases that cause the progression of vascular endothelial damage and atherothrombosis and indicates an increased risk of recurrent stroke.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77516489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.15
V. Ruden
Objective. The establishment and substantiation of biostatistical trends in the state of mortality of the population of Ukraine due to diseases of the circulatory system (I00-I99) in the context of the place of residence of the deceased for the period 2009-2019.Material and methods. An epidemiological, one-time, continuous, prospective scientific study was carried out using statistical data of the State Statistics Service of Ukraine and the Center for Medical Statistics of the Ministry of Health of Ukraine for the period 2009-2019, where the author performed the compilation and processing in spreadsheets of Microsoft Office Еxcel (2019). Retrospective, biostatistical, mathematical, abstract and graphic research methods are used, as well as methods of copying, deductive awareness, structural and logical analysis taking into account the principles of systematicity.Results and discussion. The obtained results indicate that mortality due to DCS (I00-I99), during the studied period (2009-2019), is dominant in the overall mortality structure of the population of Ukraine, where ischemic heart disease (I20, I24-I25) ranks first in terms of mortality, especially among the inhabitants of rural areas of the country, while the following rankings: second, third, fourth, consistently and intransigently share cerebrovascular disease (I60-I69), myocardial infarction (I21-I24) and hypertension (I10-I15), which are dominant in the inhabitants of urban areas.Conclusions. Given the excessive and direct burden of DCS mortality on the quantitative expression of the country's population and its negative impact on people's quality of life, as well as the state of public health, socio-economic well-being and resource provision of society, the state of Ukraine is required to make decisive and effective priority adequate management decisions of preventive content, to minimize cardiovascular pathology (I00-I99) in the context of "European guidelines for the prevention of cardiovascular disease in clinical practice" (2016).
{"title":"Apophegma on population mortality due to diseases of the circulatory system in the context of the residence of the deceased as aporia in the public life of Ukraine","authors":"V. Ruden","doi":"10.24061/2413-0737.xxv.4.100.2021.15","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.15","url":null,"abstract":"Objective. The establishment and substantiation of biostatistical trends in the state of mortality of the population of Ukraine due to diseases of the circulatory system (I00-I99) in the context of the place of residence of the deceased for the period 2009-2019.Material and methods. An epidemiological, one-time, continuous, prospective scientific study was carried out using statistical data of the State Statistics Service of Ukraine and the Center for Medical Statistics of the Ministry of Health of Ukraine for the period 2009-2019, where the author performed the compilation and processing in spreadsheets of Microsoft Office Еxcel (2019). Retrospective, biostatistical, mathematical, abstract and graphic research methods are used, as well as methods of copying, deductive awareness, structural and logical analysis taking into account the principles of systematicity.Results and discussion. The obtained results indicate that mortality due to DCS (I00-I99), during the studied period (2009-2019), is dominant in the overall mortality structure of the population of Ukraine, where ischemic heart disease (I20, I24-I25) ranks first in terms of mortality, especially among the inhabitants of rural areas of the country, while the following rankings: second, third, fourth, consistently and intransigently share cerebrovascular disease (I60-I69), myocardial infarction (I21-I24) and hypertension (I10-I15), which are dominant in the inhabitants of urban areas.Conclusions. Given the excessive and direct burden of DCS mortality on the quantitative expression of the country's population and its negative impact on people's quality of life, as well as the state of public health, socio-economic well-being and resource provision of society, the state of Ukraine is required to make decisive and effective priority adequate management decisions of preventive content, to minimize cardiovascular pathology (I00-I99) in the context of \"European guidelines for the prevention of cardiovascular disease in clinical practice\" (2016).","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"348 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75946339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.10
S. Nykytyuk, I. Klishch, S. Klymnyuk
Lyme borreliosis is an endemic disease of Western Ukraine. Infections are caused by members of the genus Borrelia.Main part. 275 children aged from 1 to 18 were examined in the laboratory of the Center for the Study of Lyme-borreliosis and other tick-borne infections.Material and methods. Serological examination of children was performed by the method of two-stage diagnosis, using ELISA primarily and confirming the results by immunoblotting. Baseline studies related to clinical and immunological examinations were performed, including data from Elisa and Immunoblot EUROLINE Borrelia RN-AT.Results. 275 children were examined. Among them were 179 children from the Ternopil region, 31 from Zhytomyr region, 30 from Khmelnytsky region, and 35 from Rivne region - aged 1-18. Children had non-specific manifestations of infection, namely, weakness, lethargy, malaise. They were screened for the presence of Ig M I IgG to B. burgdorferi s.l. Conclusions.1. The genotype of B. burgdorferi sensu stricto was found in children of some regions of Ukraine, Ternopil, Zhytomyr, Khmelnytsky, Rivne regions. 2. IgM to Ospc B. garinii antigens prevailed over Ospc b. afzelii, Ospc b. burdorferi in children of Ternopil region. 3. IgG to Vlse B. burdorferi antigens prevailed over the corresponding antigens of B. afzelii, Vlse B. garinii in children of Ternopil region. 4. The level of antibodies to antigens Ospc B. garinii, Ospc b. afzelii, Ospc b. burdorferi in the Khmelnytsky region predominate over the level of antibody antigens in other surveyed areas. 5. Compared to Ternopil oblast, Khmelnytsky oblast is the second most endemic for Lyme disease, as the level of IgG antibody titer in children of Ternopil (17.3 %) and Khmelnytsky (10 %) region are much higher than in Zhytomyr (6.4 %) and Rivne (2.8 %) area.
莱姆病是乌克兰西部的一种地方病。感染是由疏螺旋体属的成员引起的。主要部分。在莱姆病和其他蜱传感染研究中心的实验室里，对275名1至18岁的儿童进行了检查。材料和方法。患儿血清学检查采用两阶段诊断法，以ELISA为主，免疫印迹法确认结果。进行了与临床和免疫学检查相关的基线研究，包括Elisa和免疫印迹EUROLINE伯氏疏螺旋体rn - at的数据。275名儿童接受了检查。其中179名儿童来自捷尔诺波尔地区，31名来自日托米尔地区，30名来自赫梅利尼茨基地区，35名来自里夫涅地区，年龄为1-18岁。患儿感染有非特异性表现，即虚弱、嗜睡、不适。对其进行了免疫球蛋白I和IgG的检测。在乌克兰、捷尔诺波尔、日托米尔、赫梅利尼茨基、里夫涅等地区的儿童中发现了严格感伯氏疏螺旋体基因型。2. 在捷尔诺波尔地区儿童中，对格瑞尼Ospc B. garinii抗原的IgM高于阿兹利Ospc B. afzelii和伯氏Ospc B. burdorferi。3.在捷尔诺波尔地区儿童中，对伯氏疏螺旋体抗原的IgG含量高于阿夫泽利螺旋体、加里尼疏螺旋体相应抗原。4. 赫梅利尼茨基地区对抗原Ospc B. garinii、Ospc B. afzelii、Ospc B. burdorferi的抗体水平高于其他调查地区的抗体抗原水平。5. 与捷尔诺波尔州(Ternopil)相比，赫梅利尼茨基州(Khmelnytsky)是莱姆病的第二大流行地区，因为捷尔诺波尔地区(17.3%)和赫梅利尼茨基地区(10%)儿童的IgG抗体滴度远高于日托米尔地区(6.4%)和里夫涅地区(2.8%)。
{"title":"Clinical and serological features of Lyme borreliosis in different regions of Ukraine","authors":"S. Nykytyuk, I. Klishch, S. Klymnyuk","doi":"10.24061/2413-0737.xxv.4.100.2021.10","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.10","url":null,"abstract":"Lyme borreliosis is an endemic disease of Western Ukraine. Infections are caused by members of the genus Borrelia.Main part. 275 children aged from 1 to 18 were examined in the laboratory of the Center for the Study of Lyme-borreliosis and other tick-borne infections.Material and methods. Serological examination of children was performed by the method of two-stage diagnosis, using ELISA primarily and confirming the results by immunoblotting. Baseline studies related to clinical and immunological examinations were performed, including data from Elisa and Immunoblot EUROLINE Borrelia RN-AT.Results. 275 children were examined. Among them were 179 children from the Ternopil region, 31 from Zhytomyr region, 30 from Khmelnytsky region, and 35 from Rivne region - aged 1-18. Children had non-specific manifestations of infection, namely, weakness, lethargy, malaise. They were screened for the presence of Ig M I IgG to B. burgdorferi s.l. \u0000Conclusions.1. The genotype of B. burgdorferi sensu stricto was found in children of some regions of Ukraine, Ternopil, Zhytomyr, Khmelnytsky, Rivne regions. 2. IgM to Ospc B. garinii antigens prevailed over Ospc b. afzelii, Ospc b. burdorferi in children of Ternopil region. 3. IgG to Vlse B. burdorferi antigens prevailed over the corresponding antigens of B. afzelii, Vlse B. garinii in children of Ternopil region. 4. The level of antibodies to antigens Ospc B. garinii, Ospc b. afzelii, Ospc b. burdorferi in the Khmelnytsky region predominate over the level of antibody antigens in other surveyed areas. 5. Compared to Ternopil oblast, Khmelnytsky oblast is the second most endemic for Lyme disease, as the level of IgG antibody titer in children of Ternopil (17.3 %) and Khmelnytsky (10 %) region are much higher than in Zhytomyr (6.4 %) and Rivne (2.8 %) area.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83714088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.19
S. Biletskyi, T. Kazantseva, O. Petrynych, V. Petrynych
Literature data on the pathogenesis of atherosclerosis, the use of statins, ezetimibe and inhibitors of subtilisin / kexin type 9 proprotein convertase monoclonal antibodies (PCSK9) in the prevention and treatment of cardiovascular diseases have been presented.Objective - to systematize current data on the pathogenesis of atherosclerosis, the effectiveness of lipid-lowering therapy of cardiovascular diseases.Conclusion. To date, the role of dyslipidemia and inflammation in the pathogenesis of atherosclerosis, the effectiveness of statins, ezetimibe and PCSK9 inhibitors in the prevention and treatment of cardiovascular diseases have been established.
{"title":"Lipid-lowering therapy of cardiovascular diseases (literature review)","authors":"S. Biletskyi, T. Kazantseva, O. Petrynych, V. Petrynych","doi":"10.24061/2413-0737.xxv.4.100.2021.19","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.19","url":null,"abstract":"Literature data on the pathogenesis of atherosclerosis, the use of statins, ezetimibe and inhibitors of subtilisin / kexin type 9 proprotein convertase monoclonal antibodies (PCSK9) in the prevention and treatment of cardiovascular diseases have been presented.Objective - to systematize current data on the pathogenesis of atherosclerosis, the effectiveness of lipid-lowering therapy of cardiovascular diseases.Conclusion. To date, the role of dyslipidemia and inflammation in the pathogenesis of atherosclerosis, the effectiveness of statins, ezetimibe and PCSK9 inhibitors in the prevention and treatment of cardiovascular diseases have been established.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"231 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77471172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.14
M. Ryznychuk, T. Khmara, T. Komshuk, I. Zamorskii, I. Kiiun, A. Petriuk
Objective. To establish sex and age differences in the ventricular system of the brain in preschool children.Material and Methods. We examined 36 patients aged from two to five who live in the Chernivtsi region and sought medical care in MMI “Regional Children's Clinical Hospital” in Chernivtsi during 2019-2020, of which 22 were girls and 14 were boys. The analysis of the obtained results was carried out by the package "STATISTICA 5.5" (using nonparametric methods).Results. When studying the Echo-EG indices, we detected no interhemispheric asymmetry in any case. The distance to the terminal complex on the right and left was more significant in girls than boys in almost all age categories, except for the three-year-old age, here the opposite trend was found. The analysis of M-echo by age revealed a physiological increase in the index at the age of two in boys and girls. Subsequently, the distance to M-echo gradually decreases with age in boys. In girls, this index maximally reduced at the years 3 of age. Comparison of the M-echo width by age revealed a physiological increase in the index at the age of two years in both sexes with a subsequent decrease until the age of five. In all examined children, the shape of the median complex was in the form of peaks with a narrow, clear base, with one apex. Comparison by age parameters revealed a stable value of the cerebral cortex index in all age groups. In all examined children, the “pulsation” index did not exceed 20%.Conclusions. When studying the indices: distance to the terminal complex, distance to the M-echo, the width of the M-echo, a physiological increase of this index in two-year-old children with its subsequent decrease until the age of five was revealed. CCI and pulsation index practically did not change at all ages.
{"title":"Morphometric parameters of the ventricular system of the brain in preschool children","authors":"M. Ryznychuk, T. Khmara, T. Komshuk, I. Zamorskii, I. Kiiun, A. Petriuk","doi":"10.24061/2413-0737.xxv.4.100.2021.14","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.14","url":null,"abstract":"Objective. To establish sex and age differences in the ventricular system of the brain in preschool children.Material and Methods. We examined 36 patients aged from two to five who live in the Chernivtsi region and sought medical care in MMI “Regional Children's Clinical Hospital” in Chernivtsi during 2019-2020, of which 22 were girls and 14 were boys. The analysis of the obtained results was carried out by the package \"STATISTICA 5.5\" (using nonparametric methods).Results. When studying the Echo-EG indices, we detected no interhemispheric asymmetry in any case. The distance to the terminal complex on the right and left was more significant in girls than boys in almost all age categories, except for the three-year-old age, here the opposite trend was found. The analysis of M-echo by age revealed a physiological increase in the index at the age of two in boys and girls. Subsequently, the distance to M-echo gradually decreases with age in boys. In girls, this index maximally reduced at the years 3 of age. Comparison of the M-echo width by age revealed a physiological increase in the index at the age of two years in both sexes with a subsequent decrease until the age of five. In all examined children, the shape of the median complex was in the form of peaks with a narrow, clear base, with one apex. Comparison by age parameters revealed a stable value of the cerebral cortex index in all age groups. In all examined children, the “pulsation” index did not exceed 20%.Conclusions. When studying the indices: distance to the terminal complex, distance to the M-echo, the width of the M-echo, a physiological increase of this index in two-year-old children with its subsequent decrease until the age of five was revealed. CCI and pulsation index practically did not change at all ages.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87255945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-29DOI: 10.24061/2413-0737.xxv.4.100.2021.4
O. Zaliavska, O. Khukhlina, Y. Tkach, O. Nika
Disorders of metabolic processes in the "proteoglycans-collagen" system, changes in enzymatic reactions in patients with reactive arthritis (ReA) are often preceded by many complications, including impaired renal function. Classical regression methods are not very informative in determining the mutual burden of ReA and chronic pyelonephritis (CP), as well as the primacy of the disease.Objective - to investigate the possible relationship between ReA and CP in order to establish early criteria for predicting the development of CP on the background of ReA on the indicators of connective tissue metabolism. Material and methods. 113 patients were examined, which were divided into two groups: the first group - patients with urogenic ReA, activity I-III. FTS I-III st. (n = 65); the second group - patients with urogenic ReA and CKD I-II: pyelonephritis in the acute phase (n = 48). The control group consisted of 20 healthy individuals. The average duration of the disease of the examined patients was 24.4 ± 4.7 months. The mean age of patients was 32.5 ± 1.2 years. DataMining clustering and classification methods were used to process the obtained research results.Results. As a result of clustering methods (k-means and fuzzy clustering), the same results of cluster membership were obtained. In particular, ReA disease was correctly diagnosed in 48 cases, which is 74% of all diagnosed, 17 people (26%) were assigned to cluster "2". There is also a mismatch in the second cluster. In particular, out of 32 people diagnosed with ReA + CP, according to mathematical calculations, 28 people (88%) got into this cluster. Four patients (13%) were assigned to cluster "1" – to the group of persons in whom only one ReA disease should be diagnosed. This indicates that the boundary between the ReA and ReA + CP clusters are somewhat blurred. And this is the basis for establishing the fact that ReA disease can gradually lead to CP.Conclusions. Using DataMining clustering methods the greatest significance was defined in the diagnostic algorithm of ReA progression of such indicators as free oxyproline (FOP), protein-bound oxyproline (PBOP), the degree of collagenolytic activity (CLA) (intensity of azocol lysis), which showed a direct dependence on the degree of activity of the inflammatory process. Increases in FOP> 13.8 μmol/l, PBOP> 65.0 μmol/l and CLA (azocol)> 0.85 μg / ml in 1 h are probable risk factors for progression and early criteria for severe ReA and CP.
{"title":"Application of DataMining clustering and classification methods in the study of the mechanisms of mutual burdening of reactive arthritis and chronic pyelonephritis in terms of connective tissue metabolism","authors":"O. Zaliavska, O. Khukhlina, Y. Tkach, O. Nika","doi":"10.24061/2413-0737.xxv.4.100.2021.4","DOIUrl":"https://doi.org/10.24061/2413-0737.xxv.4.100.2021.4","url":null,"abstract":"Disorders of metabolic processes in the \"proteoglycans-collagen\" system, changes in enzymatic reactions in patients with reactive arthritis (ReA) are often preceded by many complications, including impaired renal function. Classical regression methods are not very informative in determining the mutual burden of ReA and chronic pyelonephritis (CP), as well as the primacy of the disease.Objective - to investigate the possible relationship between ReA and CP in order to establish early criteria for predicting the development of CP on the background of ReA on the indicators of connective tissue metabolism. Material and methods. 113 patients were examined, which were divided into two groups: the first group - patients with urogenic ReA, activity I-III. FTS I-III st. (n = 65); the second group - patients with urogenic ReA and CKD I-II: pyelonephritis in the acute phase (n = 48). The control group consisted of 20 healthy individuals. The average duration of the disease of the examined patients was 24.4 ± 4.7 months. The mean age of patients was 32.5 ± 1.2 years. DataMining clustering and classification methods were used to process the obtained research results.Results. As a result of clustering methods (k-means and fuzzy clustering), the same results of cluster membership were obtained. In particular, ReA disease was correctly diagnosed in 48 cases, which is 74% of all diagnosed, 17 people (26%) were assigned to cluster \"2\". There is also a mismatch in the second cluster. In particular, out of 32 people diagnosed with ReA + CP, according to mathematical calculations, 28 people (88%) got into this cluster. Four patients (13%) were assigned to cluster \"1\" – to the group of persons in whom only one ReA disease should be diagnosed. This indicates that the boundary between the ReA and ReA + CP clusters are somewhat blurred. And this is the basis for establishing the fact that ReA disease can gradually lead to CP.Conclusions. Using DataMining clustering methods the greatest significance was defined in the diagnostic algorithm of ReA progression of such indicators as free oxyproline (FOP), protein-bound oxyproline (PBOP), the degree of collagenolytic activity (CLA) (intensity of azocol lysis), which showed a direct dependence on the degree of activity of the inflammatory process. Increases in FOP> 13.8 μmol/l, PBOP> 65.0 μmol/l and CLA (azocol)> 0.85 μg / ml in 1 h are probable risk factors for progression and early criteria for severe ReA and CP.","PeriodicalId":9270,"journal":{"name":"Bukovinian Medical Herald","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89148275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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