The formulation of effective air quality and ventilation standards for sleep environment depends on fundamental data of human emission characteristics and a clear understanding of their impact on sleep quality. This study quantified CO2 and VOC concentrations in thermally neutral confined bedrooms under three typical occupant densities, monitoring sleep stages, environmental perceptions, and physiological responses in eight healthy adults. Results showed an inverse relationship between per-capita CO2 emission rate and CO2 exposure concentration. Relative to the baseline concentration (1,063 ± 182 ppm), this relationship necessitated correction factors of 0.79 and 0.73 for mean indoor CO2 levels of 1,351 ± 343 ppm and 2,194 ± 623 ppm, respectively. Air analysis indicated that 94 % of dual-source human-material compounds (25 compounds across five categories) accumulated with increasing occupant density. Notably, total emission mass concentrations of dual-origin VOCs significantly exceeded those from materials alone: non-benzenoid hydrocarbons by 10-fold, benzenoid hydrocarbons by 3–4 times, and halogenated compounds/esters by 1.5 times. When the average indoor CO₂ concentration at night was 2,194 ± 623 ppm (with a peak of 2,838 ppm), sleep efficiency declined to 86.3 %, vagal nerve hyperactivity manifested, and post-wake systolic blood pressure increased. To ensure sleep quality and health, average CO2 concentration of bedroom should remain below 2200 ppm (peak less than 3000 ppm). VOC purification should prioritize non-benzenoid hydrocarbons (less than 65 μg/m3), benzenoid hydrocarbons (less than 225 μg/m3), halogenated compounds (less than 95 μg/m3), and esters (less than 55 μg/m3), with lower concentrations preferred.
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