Amanda C Meadows, S. Terry, G. Penner, P. Hucl, T. McAllister, G. Ribeiro
Abstract This study assessed the impact of dry- (DR) versus temper-rolled (TR) and low- (13%, LP) versus high-protein (18%, HP) wheat on ruminal fermentation, growth, and liver abscesses in feedlot cattle. Crossbred Angus steers (302 ± 34 kg; n = 160; 24 ruminally cannulated) were used in a backgrounding (BG) to finishing (FN) trial. The BG diet consisted of 60% barley silage, 35% wheat, and 5% supplement, and the FN diet contained 10% barley silage, 85% wheat, and 5% supplement (dry matter basis). Four transition (TN) diets were used to adapt cattle to the FN diet. A numerical increase in large particles and reduction in small particles occurred when both HP and LP wheat were TR, with this response being greater for HP wheat. Steers experienced lower (P ≤ 0.03) ruminal pH with HP-DR and LP-TR than HP-TR wheat during TN. Steers fed HP wheat BG diets tended to exhibit greater (P ≤ 0.09) gain:feed and NEg than steers fed LP wheat. Greater (P = 0.01) average daily gains were exhibited by FN steers fed LP wheat. Liver abscesses were more (P < 0.001) severe with HP wheat. While HP wheat improved the growth of BG cattle, it increased the severity of liver abscesses during FN.
{"title":"Effect of dry or temper rolling of high- or low-protein wheat and its impact on rumen parameters, growth performance, and liver abscesses in feedlot cattle","authors":"Amanda C Meadows, S. Terry, G. Penner, P. Hucl, T. McAllister, G. Ribeiro","doi":"10.1139/cjas-2022-0110","DOIUrl":"https://doi.org/10.1139/cjas-2022-0110","url":null,"abstract":"Abstract This study assessed the impact of dry- (DR) versus temper-rolled (TR) and low- (13%, LP) versus high-protein (18%, HP) wheat on ruminal fermentation, growth, and liver abscesses in feedlot cattle. Crossbred Angus steers (302 ± 34 kg; n = 160; 24 ruminally cannulated) were used in a backgrounding (BG) to finishing (FN) trial. The BG diet consisted of 60% barley silage, 35% wheat, and 5% supplement, and the FN diet contained 10% barley silage, 85% wheat, and 5% supplement (dry matter basis). Four transition (TN) diets were used to adapt cattle to the FN diet. A numerical increase in large particles and reduction in small particles occurred when both HP and LP wheat were TR, with this response being greater for HP wheat. Steers experienced lower (P ≤ 0.03) ruminal pH with HP-DR and LP-TR than HP-TR wheat during TN. Steers fed HP wheat BG diets tended to exhibit greater (P ≤ 0.09) gain:feed and NEg than steers fed LP wheat. Greater (P = 0.01) average daily gains were exhibited by FN steers fed LP wheat. Liver abscesses were more (P < 0.001) severe with HP wheat. While HP wheat improved the growth of BG cattle, it increased the severity of liver abscesses during FN.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42909452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The present study was carried out to evaluate the effect of cinnamon and Bacillus subtilis on the growth, meat characteristics, and lipid profiles of broiler chickens. In experiments 1 and 2, a total of 320 day-old broiler chicks were assigned to D1 (control), D2 (4 g cinnamon/kg), D3 (6 g cinnamon/kg), D4 (8 g cinnamon/kg) with four replicates, and D1 (control), D2 (0.4 g B. subtilis (BS)/kg), D3 (0.6 g BS/kg), D4 (0.8 g BS/kg), D5 (6 g cinnamon/kg) with three replicates, and 10 chicks/replication for 35 and 30 days, respectively. In experiment 1, no significant difference was observed among diets for growth and meat yield. However, test diets performed better than the control diet in terms of water-holding capacity of meat (WHCM), cooking loss of meat (CLM), blood lipids profiles (BLP), sensory quality score (SQS) (p > 0.05), and flavor (p < 0.05). In experiment 2, there was no significant difference among diets for growth, meat yield, WHCM, CLM, and BLP of the bird (p > 0.05), except for SQS (p < 0.001). Notwithstanding, the D5 tended to increase growth, meat yield, WHCM, and SQS, and decrease CLM and BLP. Thigh meat showed higher SQS than breast meat in both experiments. Therefore, the 6 g cinnamon/kg diet may be used for producing a safe, quality, and cost-effective broiler.
{"title":"Use of cinnamon and Bacillus subtilis probiotics in the diet of broiler chickens","authors":"Mohammad Aminul Islam, M. Nishibori","doi":"10.1139/cjas-2022-0018","DOIUrl":"https://doi.org/10.1139/cjas-2022-0018","url":null,"abstract":"Abstract The present study was carried out to evaluate the effect of cinnamon and Bacillus subtilis on the growth, meat characteristics, and lipid profiles of broiler chickens. In experiments 1 and 2, a total of 320 day-old broiler chicks were assigned to D1 (control), D2 (4 g cinnamon/kg), D3 (6 g cinnamon/kg), D4 (8 g cinnamon/kg) with four replicates, and D1 (control), D2 (0.4 g B. subtilis (BS)/kg), D3 (0.6 g BS/kg), D4 (0.8 g BS/kg), D5 (6 g cinnamon/kg) with three replicates, and 10 chicks/replication for 35 and 30 days, respectively. In experiment 1, no significant difference was observed among diets for growth and meat yield. However, test diets performed better than the control diet in terms of water-holding capacity of meat (WHCM), cooking loss of meat (CLM), blood lipids profiles (BLP), sensory quality score (SQS) (p > 0.05), and flavor (p < 0.05). In experiment 2, there was no significant difference among diets for growth, meat yield, WHCM, CLM, and BLP of the bird (p > 0.05), except for SQS (p < 0.001). Notwithstanding, the D5 tended to increase growth, meat yield, WHCM, and SQS, and decrease CLM and BLP. Thigh meat showed higher SQS than breast meat in both experiments. Therefore, the 6 g cinnamon/kg diet may be used for producing a safe, quality, and cost-effective broiler.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45820377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Cargo-Froom, F. Tansil, D. Columbus, C. Marinangeli, E. Kiarie, A. Shoveller
Abstract The objective of this study was to determine the standardized ileal digestibility (SID) of protein and amino acids (AA) and calculate the digestible indispensable AA score (DIAAS) of three varieties of Canadian grown pulses (faba bean, lentil, and yellow pea). Three steam-pelleted (80 °C) diets (faba/lentil/pea; 40% inclusion) and a nitrogen-free diet were fed to eight cannulated growing pigs in a 4 × 4 replicated Latin square design. The SID values were used to calculate DIAAS. There were no differences in SID of protein and AA when all diets were compared (P > 0.05). The SID of methionine was 95% for faba beans, 96% for lentils, and 96% for peas. The SID of lysine was 93% for faba beans, 92% for lentils, and 90% for peas. Tryptophan had the lowest SID across all indispensable AA of all pulses (59% faba, 61% lentil, and 41% pea). All other indispensable AA SID were above 80%. Tryptophan was the limiting indispensable AA for all pulses as determined by DIAAS, with scores of 30 (faba), 57.83 (lentil), and 27.27 (pea). These values will aid in optimal inclusion of steam-pelleted pulses in diets for swine and provide an insight on the protein quality of pulses for humans.
{"title":"Determination of standardized ileal digestibility of crude protein and amino acids and digestible indispensable amino acid score of faba beans, lentils, and yellow peas fed to growing pigs","authors":"C. Cargo-Froom, F. Tansil, D. Columbus, C. Marinangeli, E. Kiarie, A. Shoveller","doi":"10.1139/cjas-2023-0022","DOIUrl":"https://doi.org/10.1139/cjas-2023-0022","url":null,"abstract":"Abstract The objective of this study was to determine the standardized ileal digestibility (SID) of protein and amino acids (AA) and calculate the digestible indispensable AA score (DIAAS) of three varieties of Canadian grown pulses (faba bean, lentil, and yellow pea). Three steam-pelleted (80 °C) diets (faba/lentil/pea; 40% inclusion) and a nitrogen-free diet were fed to eight cannulated growing pigs in a 4 × 4 replicated Latin square design. The SID values were used to calculate DIAAS. There were no differences in SID of protein and AA when all diets were compared (P > 0.05). The SID of methionine was 95% for faba beans, 96% for lentils, and 96% for peas. The SID of lysine was 93% for faba beans, 92% for lentils, and 90% for peas. Tryptophan had the lowest SID across all indispensable AA of all pulses (59% faba, 61% lentil, and 41% pea). All other indispensable AA SID were above 80%. Tryptophan was the limiting indispensable AA for all pulses as determined by DIAAS, with scores of 30 (faba), 57.83 (lentil), and 27.27 (pea). These values will aid in optimal inclusion of steam-pelleted pulses in diets for swine and provide an insight on the protein quality of pulses for humans.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48569859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hajer Sammari, Amani Askri, Sahar Benahmed, L. Saucier, Nabeel Alnahhas
Abstract In this study, 206 breast fillets were purchased from grocery stores in the province of Quebec and evaluated for the presence of different quality defects. Of these fillets, 48.5% showed breast muscle myopathies (BMM), 19.4% showed pale, soft, and exudative (PSE), and 6.8% showed dark, firm, and dry (DFD) attributes. BMM were equally present (P > 0.05) in fillets of economical, commercial, and high-quality brands, while PSE-like fillets were more present in economical brands (P < 0.0001). The combined effect of BMM and DFD induced significantly higher counts of Salmonella (P = 0.03) and Enterobacteriaceae (P = 0.03) in myopathic than in unaffected fillets. These quality defects also altered the nutritional quality of breast meat: BMM-affected fillets had greater fat content (P < 0.0001) and DFD fillets had lower protein content (P = 0.041) than normal fillets. The technological quality was only slightly impacted by BMM, while PSE-like fillets had higher cooking loss (P = 0.009) and a tougher texture after cooking (P < 0.0001) than DFD fillets. For the first time, this study confirmed the presence of multiple quality issues in the Quebec poultry supply chain, and provided valuable data to support future research efforts.
{"title":"A survey of broiler breast meat quality in the retail market of Quebec","authors":"Hajer Sammari, Amani Askri, Sahar Benahmed, L. Saucier, Nabeel Alnahhas","doi":"10.1139/cjas-2023-0001","DOIUrl":"https://doi.org/10.1139/cjas-2023-0001","url":null,"abstract":"Abstract In this study, 206 breast fillets were purchased from grocery stores in the province of Quebec and evaluated for the presence of different quality defects. Of these fillets, 48.5% showed breast muscle myopathies (BMM), 19.4% showed pale, soft, and exudative (PSE), and 6.8% showed dark, firm, and dry (DFD) attributes. BMM were equally present (P > 0.05) in fillets of economical, commercial, and high-quality brands, while PSE-like fillets were more present in economical brands (P < 0.0001). The combined effect of BMM and DFD induced significantly higher counts of Salmonella (P = 0.03) and Enterobacteriaceae (P = 0.03) in myopathic than in unaffected fillets. These quality defects also altered the nutritional quality of breast meat: BMM-affected fillets had greater fat content (P < 0.0001) and DFD fillets had lower protein content (P = 0.041) than normal fillets. The technological quality was only slightly impacted by BMM, while PSE-like fillets had higher cooking loss (P = 0.009) and a tougher texture after cooking (P < 0.0001) than DFD fillets. For the first time, this study confirmed the presence of multiple quality issues in the Quebec poultry supply chain, and provided valuable data to support future research efforts.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44302123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Boonstra, I. Aboagye, T. McAllister, G. Legesse, G. Mengistu, D. L. Fulawka, Marcos R. C. Cordeiro, G. Ribeiro, E. McGeough, K. Ominski
Abstract Greenhouse gas (GHG) and ammonia (NH3) emissions, land and water use associated with feedlot cattle (n = 40 hd treatment−1 trial−1) treated with or without productivity-enhancing technologies were modelled for a multiyear study (n = 4). Heifers (H) were assigned to the following treatments: (1) implanted (HTBA); (2) provided with melengestrol acetate (HMGA); (3) nonimplanted control, weight-adjusted (CON_Adj) to achieve the same final carcass weight (CW) as 1 (HCON_AdjTBA); or (4) CON_Adj to achieve the CW as 2 (HCON_AdjMGA). Steers (S) were assigned as follows: (1) implanted (STBA); (2) implanted and provided with ractopamine hydrochloride (SRAC; conducted in the last 2 years); (3) CON_Adj to achieve the same CW as 1 (SCON_AdjTBA); or (4) CON_Adj to achieve the same CW as 2 (SCON_AdjRAC). The GHG and NH3 emissions from HTBA, HMGA, STBA, and SRAC were 3.8%, 3.0%, 10.1%, and 8.5% lower and 4.3%, 2.9%, 7.4%, and 7.6% lower, respectively, than the respective control cattle. The land required to produce feed was also reduced by 6.6%, 4.8%, 9.9%, and 10.9%, while water use was reduced by 6.4%, 4.8%, 10.1%, and 11.1% for HTBA, HMGA, STBA, and SRAC, respectively. This modelling study clearly demonstrates that conventional beef production systems have a lower environmental footprint than nonconventional systems.
{"title":"Modelling environmental impacts associated with the removal of productivity-enhancing technologies from Canadian feedlots: a case study","authors":"E. Boonstra, I. Aboagye, T. McAllister, G. Legesse, G. Mengistu, D. L. Fulawka, Marcos R. C. Cordeiro, G. Ribeiro, E. McGeough, K. Ominski","doi":"10.1139/cjas-2022-0132","DOIUrl":"https://doi.org/10.1139/cjas-2022-0132","url":null,"abstract":"Abstract Greenhouse gas (GHG) and ammonia (NH3) emissions, land and water use associated with feedlot cattle (n = 40 hd treatment−1 trial−1) treated with or without productivity-enhancing technologies were modelled for a multiyear study (n = 4). Heifers (H) were assigned to the following treatments: (1) implanted (HTBA); (2) provided with melengestrol acetate (HMGA); (3) nonimplanted control, weight-adjusted (CON_Adj) to achieve the same final carcass weight (CW) as 1 (HCON_AdjTBA); or (4) CON_Adj to achieve the CW as 2 (HCON_AdjMGA). Steers (S) were assigned as follows: (1) implanted (STBA); (2) implanted and provided with ractopamine hydrochloride (SRAC; conducted in the last 2 years); (3) CON_Adj to achieve the same CW as 1 (SCON_AdjTBA); or (4) CON_Adj to achieve the same CW as 2 (SCON_AdjRAC). The GHG and NH3 emissions from HTBA, HMGA, STBA, and SRAC were 3.8%, 3.0%, 10.1%, and 8.5% lower and 4.3%, 2.9%, 7.4%, and 7.6% lower, respectively, than the respective control cattle. The land required to produce feed was also reduced by 6.6%, 4.8%, 9.9%, and 10.9%, while water use was reduced by 6.4%, 4.8%, 10.1%, and 11.1% for HTBA, HMGA, STBA, and SRAC, respectively. This modelling study clearly demonstrates that conventional beef production systems have a lower environmental footprint than nonconventional systems.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44680618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Babatunde, C. Cargo-Froom, Y. Ai, R. Newkirk, C. Marinangeli, A. Shoveller, D. Columbus
Abstract Pulses are important as alternative sources of protein and carbohydrates for the animal industry and, thus, require accurate evaluation of their nutrient profile during processing. Extrusion is a thermal processing of ingredients to induce physiochemical changes that convert them into more valuable products. The current study evaluated the effects of extrusion on the starch and fibre components of Amarillo peas, Dun peas, chickpeas, faba beans, lentils, and soybean meal (SBM). Pulses were extruded at 18% or 22% moisture and 110, 130, or 150 °C. Extrusion decreased (P < 0.05) the starch content in Amarillo and Dun peas but increased (P < 0.05) the same in faba beans, lentils, and SBM when compared with their whole counterparts. There was no difference in the total dietary fibre content of whole and extruded Amarillo peas, Dun peas, chickpeas, and SBM. Extrusion increased (P < 0.05) the soluble dietary fibre (SDF) content of all pulses and SBM except chickpeas. Extrusion increased (P < 0.05) for all fibre types in faba beans. Results indicate that extrusion increased the starch and SDF content of most pulses but had negative or no effects on other fibre components in all pulses except faba beans.
{"title":"Extrusion effects on the starch and fibre composition of Canadian pulses","authors":"O. Babatunde, C. Cargo-Froom, Y. Ai, R. Newkirk, C. Marinangeli, A. Shoveller, D. Columbus","doi":"10.1139/cjas-2022-0127","DOIUrl":"https://doi.org/10.1139/cjas-2022-0127","url":null,"abstract":"Abstract Pulses are important as alternative sources of protein and carbohydrates for the animal industry and, thus, require accurate evaluation of their nutrient profile during processing. Extrusion is a thermal processing of ingredients to induce physiochemical changes that convert them into more valuable products. The current study evaluated the effects of extrusion on the starch and fibre components of Amarillo peas, Dun peas, chickpeas, faba beans, lentils, and soybean meal (SBM). Pulses were extruded at 18% or 22% moisture and 110, 130, or 150 °C. Extrusion decreased (P < 0.05) the starch content in Amarillo and Dun peas but increased (P < 0.05) the same in faba beans, lentils, and SBM when compared with their whole counterparts. There was no difference in the total dietary fibre content of whole and extruded Amarillo peas, Dun peas, chickpeas, and SBM. Extrusion increased (P < 0.05) the soluble dietary fibre (SDF) content of all pulses and SBM except chickpeas. Extrusion increased (P < 0.05) for all fibre types in faba beans. Results indicate that extrusion increased the starch and SDF content of most pulses but had negative or no effects on other fibre components in all pulses except faba beans.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45199563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kai Wang, Xin Ren, X. Shen, Yansen Li, Yangchun Xia, Zhaojian Li, Chunmei Li
Abstract This experiment evaluated the effects of sugarcane extract (SCE) on growing pigs' diarrhea incidence, serum immunity, intestinal morphology, and antioxidant enzyme activity. A total of 116 52-day-old commercial pigs (Duroc × Landrace × Jiaxing Black, average weight of 11 ± 1 kg) were randomly allocated to control (CON, basal diet) and 1% SCE group (SCEG, basal diet + 1% SCE). The experiment lasted four weeks. Compared with CON, diarrhea incidence (SCEG: 74.11% versus CON: 54.76%) and diarrhea index (SCEG: 83.65% versus CON: 73.61%) dropped largely in pigs supplemented with SCE. Villus height in the duodenum (p < 0.01) and the ratio of villus height to crypt depth (p < 0.05) increased in SCEG. Dietary SCE enhanced the activity of catalase (CAT) capacity, and decreased tumor necrosis factor α, and malondialdehyde levels in serum (p < 0.05). CAT activity in the ileum increased (p < 0.05) in piglets supplemented with SCE. Thus, dietary supplementation with SCE improved diarrhea incidence, serum antioxidant capacity and immunity, and intestinal villus morphology and may be used as an efficient antibiotic alternative in weaned piglet feed.
{"title":"Effects of sugarcane extract on growth and diarrhea of growing piglets","authors":"Kai Wang, Xin Ren, X. Shen, Yansen Li, Yangchun Xia, Zhaojian Li, Chunmei Li","doi":"10.1139/cjas-2022-0136","DOIUrl":"https://doi.org/10.1139/cjas-2022-0136","url":null,"abstract":"Abstract This experiment evaluated the effects of sugarcane extract (SCE) on growing pigs' diarrhea incidence, serum immunity, intestinal morphology, and antioxidant enzyme activity. A total of 116 52-day-old commercial pigs (Duroc × Landrace × Jiaxing Black, average weight of 11 ± 1 kg) were randomly allocated to control (CON, basal diet) and 1% SCE group (SCEG, basal diet + 1% SCE). The experiment lasted four weeks. Compared with CON, diarrhea incidence (SCEG: 74.11% versus CON: 54.76%) and diarrhea index (SCEG: 83.65% versus CON: 73.61%) dropped largely in pigs supplemented with SCE. Villus height in the duodenum (p < 0.01) and the ratio of villus height to crypt depth (p < 0.05) increased in SCEG. Dietary SCE enhanced the activity of catalase (CAT) capacity, and decreased tumor necrosis factor α, and malondialdehyde levels in serum (p < 0.05). CAT activity in the ileum increased (p < 0.05) in piglets supplemented with SCE. Thus, dietary supplementation with SCE improved diarrhea incidence, serum antioxidant capacity and immunity, and intestinal villus morphology and may be used as an efficient antibiotic alternative in weaned piglet feed.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46975906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Methylsulfonylmethane (MSM) is a natural organic sulfur component that has anti-inflammatory and antioxidant properties. In this study, injury of porcine intestinal epithelial cell (IPEC-J2) models were used to investigate the effect of MSM on lipopolysaccharide (LPS)-induced porcine intestinal epithelium barrier damage. The results of the cell cycle showed that the cells in the G2/M phase decreased significantly with the supplementation of 300 mmol/L MSM (P < 0.05). The ELISA assay revealed that MSM could significantly inhibit the expression of tumor necrosis factor-alpha, interleukin-1, and interleukin-6 (P < 0.01). Meanwhile, MSM could significantly increase the value of cell monolayer transepithelial electrical resistance while reducing the FITC-dextran flux permeability and lactate dehydrogenase activity in IPEC-J2 cells (P < 0.01). Additionally, 300 mmol/L MSM significantly increased both mRNA and protein expression of occludin, claudin-1, and ZO-1 (P < 0.05). Furthermore, MSM prevented the downregulation of epidermal growth factor receptor (EGFR) by LPS, indicating that MSM might enhance tight junction function through mechanisms of activation of EGFR-mediated protein synthesis in IPEC-J2 cells. Therefore, our findings suggested that MSM has protective effects on inflammation and epithelial barrier injury in LPS-induced IPEC-J2 cells, indicating that MSM might be used as a potential therapeutic agent in the pig industry.
{"title":"Protective effects of methylsulfonylmethane (MSM) on barrier function injury of porcine intestinal epithelial cells (IPEC-J2) induced by lipopolysaccharide (LPS)","authors":"Y. Jiao, Hengjiang Li, Ting Ren, I. Kim","doi":"10.1139/cjas-2022-0141","DOIUrl":"https://doi.org/10.1139/cjas-2022-0141","url":null,"abstract":"Abstract Methylsulfonylmethane (MSM) is a natural organic sulfur component that has anti-inflammatory and antioxidant properties. In this study, injury of porcine intestinal epithelial cell (IPEC-J2) models were used to investigate the effect of MSM on lipopolysaccharide (LPS)-induced porcine intestinal epithelium barrier damage. The results of the cell cycle showed that the cells in the G2/M phase decreased significantly with the supplementation of 300 mmol/L MSM (P < 0.05). The ELISA assay revealed that MSM could significantly inhibit the expression of tumor necrosis factor-alpha, interleukin-1, and interleukin-6 (P < 0.01). Meanwhile, MSM could significantly increase the value of cell monolayer transepithelial electrical resistance while reducing the FITC-dextran flux permeability and lactate dehydrogenase activity in IPEC-J2 cells (P < 0.01). Additionally, 300 mmol/L MSM significantly increased both mRNA and protein expression of occludin, claudin-1, and ZO-1 (P < 0.05). Furthermore, MSM prevented the downregulation of epidermal growth factor receptor (EGFR) by LPS, indicating that MSM might enhance tight junction function through mechanisms of activation of EGFR-mediated protein synthesis in IPEC-J2 cells. Therefore, our findings suggested that MSM has protective effects on inflammation and epithelial barrier injury in LPS-induced IPEC-J2 cells, indicating that MSM might be used as a potential therapeutic agent in the pig industry.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42932606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Olanrewaju, J. Purswell, J. Evans, S. Collier, S. Branton
Abstract Light impacts layer hen's welfare and production. This study investigated the effects of a novel LED light system on blood physiological variables in laying hens. In each of the two trials, 320-layer pullets were allocated to 16 groups of 20 birds. Treatments included the prototype LED lighting (two rooms) and the conventional 2700 K LED bulbs (two rooms). On each sampling day, blood samples were collected and analyzed immediately. Results show that only a few physiological variables were affected by treatments, but age had effects on most examined variables. No effects of treatments or age on plasma corticosterone signify a similar level of physiological stress.
光照影响蛋鸡的福利和生产。本研究研究了一种新型LED光系统对蛋鸡血液生理指标的影响。在两个试验中，每个试验将320个蛋鸡分配给16组，每组20只鸡。处理包括原型LED照明(两个房间)和传统的2700 K LED灯泡(两个房间)。在每个采样日，立即采集血样并进行分析。结果表明，只有少数生理变量受到治疗的影响，但年龄对大多数被检查的变量有影响。治疗或年龄对血浆皮质酮没有影响，表明生理应激水平相似。
{"title":"Age-related effects of high-frequency LED lighting in laying hens. Part 1: blood physiological variables","authors":"H. Olanrewaju, J. Purswell, J. Evans, S. Collier, S. Branton","doi":"10.1139/cjas-2022-0119","DOIUrl":"https://doi.org/10.1139/cjas-2022-0119","url":null,"abstract":"Abstract Light impacts layer hen's welfare and production. This study investigated the effects of a novel LED light system on blood physiological variables in laying hens. In each of the two trials, 320-layer pullets were allocated to 16 groups of 20 birds. Treatments included the prototype LED lighting (two rooms) and the conventional 2700 K LED bulbs (two rooms). On each sampling day, blood samples were collected and analyzed immediately. Results show that only a few physiological variables were affected by treatments, but age had effects on most examined variables. No effects of treatments or age on plasma corticosterone signify a similar level of physiological stress.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63967921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. van Staaveren, E. Hyland, K. Houlahan, C. Lynch, F. Miglior, D. Kelton, F. Schenkel, C. Baes
Abstract Calf diseases remain a challenge for dairy producers from both an economic and welfare perspective. Genetically selecting for disease resistance in calves is a promising approach that could contribute to sustainable dairy farming. Genetic evaluations, however, require well-defined and consistently recorded phenotypes to be successful. Therefore, this study aimed to understand the current state of calf disease recording on Ontario farms. Calf disease records of respiratory illness and diarrhea were available from the national milk recording organization (Lactanet Canada, Guelph, Ontario, Canada) from 2009 to 2020. A case study was conducted to describe calf disease diagnoses and recording practices by surveying a subset of 13 Ontario dairy producers. The percentage of milk recorded farms that recorded calf respiratory illness and calf diarrhea increased from 2.6% in 2009 to 11.1% in 2020. Potential sources of data loss were identified along the information chain from farm to genetic evaluation database. Clear definitions and thresholds to diagnose calf disease, standard operating procedures for data recording, as well as a data transfer pipeline, which includes exchange formats, are needed to facilitate the inclusion of calf health traits in genetic evaluations.
{"title":"Recording of calf diseases for potential use in breeding programs: a case study on calf respiratory illness and diarrhea","authors":"N. van Staaveren, E. Hyland, K. Houlahan, C. Lynch, F. Miglior, D. Kelton, F. Schenkel, C. Baes","doi":"10.1139/cjas-2022-0112","DOIUrl":"https://doi.org/10.1139/cjas-2022-0112","url":null,"abstract":"Abstract Calf diseases remain a challenge for dairy producers from both an economic and welfare perspective. Genetically selecting for disease resistance in calves is a promising approach that could contribute to sustainable dairy farming. Genetic evaluations, however, require well-defined and consistently recorded phenotypes to be successful. Therefore, this study aimed to understand the current state of calf disease recording on Ontario farms. Calf disease records of respiratory illness and diarrhea were available from the national milk recording organization (Lactanet Canada, Guelph, Ontario, Canada) from 2009 to 2020. A case study was conducted to describe calf disease diagnoses and recording practices by surveying a subset of 13 Ontario dairy producers. The percentage of milk recorded farms that recorded calf respiratory illness and calf diarrhea increased from 2.6% in 2009 to 11.1% in 2020. Potential sources of data loss were identified along the information chain from farm to genetic evaluation database. Clear definitions and thresholds to diagnose calf disease, standard operating procedures for data recording, as well as a data transfer pipeline, which includes exchange formats, are needed to facilitate the inclusion of calf health traits in genetic evaluations.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46934247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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