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Identifi cation of the Postharvest Pink Mold Rot Fungus (Trichothecium roseum) on Grapes in China 中国葡萄采后粉红霉腐菌的鉴定
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-07-31 DOI: 10.12982/cmjs.2023.040
D. Harishchandra, Wei Zhang, Thilini Chethana Kandawatte Wedaralalage, Xinghong Li, R. Cheewangkoon
Grape is an important crop due to its consumption as fresh fruit as well as the secondary products developed from the berries such as wines, jams, juice, jellies, and vinegar. Postharvest losses in grapes can adversely affect their market value and the quality of the secondary products developed from grapes. These losses can be due to pathogenic infections and other damage due to improper handling and storage practices. During a brief survey of the cold storage facilities of the Beijing Academy of Forestry and Pomology Sciences, grape berries showing postharvest rot symptoms were collected. Further onsite observations suggested that the disease was postharvest pink mold rot caused by Trichothecium roseum. Trichothecium roseum is an asexually reproducing soil-borne fungus with an unknown sexual morph. This fungus is known to cause postharvest diseases in many fruits and vegetables around the world. Our objective in this study was to report the fi rst occurrence of pink rot on grapes caused by T. roseum with molecular phylogeny, morphological studies, and pathogenicity tests. Also, this is the fi rst report of T. roseum from grapes in China.
葡萄是一种重要的作物,因为它既可以作为新鲜水果食用,也可以作为葡萄酒、果酱、果汁、果冻和醋等浆果的副产品。葡萄采后的损失会对其市场价值和由葡萄开发的二次产品的质量产生不利影响。这些损失可能是由于致病性感染和由于处理和储存不当造成的其他损害。在对北京市林业与果树科学研究院冷库设施进行的一次简短调查中,收集到有采后腐病症状的葡萄浆果。进一步的现场观察表明,该病害是由玫瑰毛霉引起的采后粉霉病。玫瑰毛霉是一种无性繁殖的土传真菌,性别形态未知。众所周知,这种真菌在世界各地的许多水果和蔬菜中引起采后疾病。通过分子系统发育、形态学研究和致病性试验,首次报道了葡萄红腐病的发生。同时,这也是中国首次报道从葡萄中分离出的roseum。
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引用次数: 0
Future Projections of Precipitation and Temperature in Northeast, Thailand using Bias-Corrected Global Climate Models 利用偏差校正的全球气候模式对泰国东北部降水和温度的未来预估
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-07-31 DOI: 10.12982/cmjs.2023.041
Wittanan Tammadid, Ketmanee Nantasom, Wichayaporn Sirksiri, Supika Vanitchung, Chaiwatchara Promjittiphong, A. Limsakul, P. Hanpattanakit
The increase in anthropogenic greenhouse gases (GHGs) has resulted in global climate change, with the Northeast region of Thailand experiencing the highest rate of change. This poses signifi cant risks of drought and its impact on crop yield production. To better understand the potential consequences of climate change and devise suitable adaptation strategies, this study aims to project precipitation and temperature data for Northeast Thailand from 2015 to 2055, using bias-corrected global climate models under Representative Concentration Pathways (RCPs) 4.5 and 8.5. The results showed that the annual precipitation and temperature increased from 2015 to 2055 under both RCPs. The annual average (minimum-maximum) of precipitation from the CanESM, GFDL, MIROC5, NorESM, and ensemble mean from four models under RCP4.5 were 1,238.83 (904.28-1,629.70), 1,227.82 (865.49- 1,721.31), 1,312.78 (876.60-1,616.38), 1,350.21 (985.55-1,625.00), and 1,282.41 (1,088.43-1,461.49) mm and RCP8.5 were 1,267.96 (864.24-1,712.86), 1,222.20 (863.79-1,835.43), 1,294.07 (843.04-1,752.41), 1,353.14 (1,059.50-1,827.23), and 1,284.34 (1,116.55-1,541.63) mm, respectively. While, those of temperature under RCP4.5 were 29.27 (28.15-30.35), 29.59 (27.98-31.20), 29.12 (27.65-30.70), 28.09 (26.96-29.14), and 29.02 (28.08-29.90) °C and RCP8.5 were 29.50 (28.12-31.40), 29.68 (28.23-31.50), 29.11 (27.51-30.26), 28.37 (27.11-29.88), and 29.17 (28.08-30.66) °C, respectively. These fi ndings suggest that the annual precipitation is lower under RCP4.5 compared to RCP8.5, while the temperature shows an increasing trend under both RCPs. Therefore, it is evident that climate change will manifest differently in Northeast Thailand, depending on local contexts and the measures implemented today. Understanding the impacts and risks of future climate change at a local scale and identifying adaptive solutions pose signifi cant challenges for the future.
人为温室气体(GHGs)的增加导致了全球气候变化,其中泰国东北部地区的变化率最高。这构成了干旱的重大风险及其对作物产量的影响。为了更好地了解气候变化的潜在后果并制定适当的适应策略,本研究旨在利用代表性浓度路径(rcp) 4.5和8.5下的偏差校正全球气候模型预测2015年至2055年泰国东北部的降水和温度数据。结果表明:2015 ~ 2055年,两种rcp条件下的年降水量和气温均呈上升趋势;年平均(最小最大)的降水CanESM, GFDL, MIROC5, NorESM,和合奏意味着从四个模型在RCP4.5 1238 .83点(904.28)-1629 .70美元,1227 .82(865.49 - 1721 .31点),1312(876.60 -1616 .38点),1350 .21(985.55 -1625),和1282 .41点(1088点- 1461 .49)毫米和RCP8.5 1267 .96点(864.24 -1712 .86点),1222 .20 -1835点(863.79),1294 .07点(843.04 -1752 .41点),1353 .14点(- 1827 .23 1059 .50),和1284 .34(- 1541 .63 1116 .55)毫米,分别。RCP4.5下的温度分别为29.27(28.15 ~ 30.35)、29.59(27.98 ~ 31.20)、29.12(27.65 ~ 30.70)、28.09(26.96 ~ 29.14)、29.02(28.08 ~ 29.90)℃,RCP8.5下的温度分别为29.50(28.12 ~ 31.40)、29.68(28.23 ~ 31.50)、29.11(27.51 ~ 30.26)、28.37(27.11 ~ 29.88)、29.17(28.08 ~ 30.66)℃。这些结果表明,RCP4.5条件下的年降水量比RCP8.5条件下的少,而两种rcpcp条件下的气温均呈上升趋势。因此,很明显,气候变化在泰国东北部的表现会有所不同,这取决于当地的情况和今天实施的措施。了解未来气候变化在局部范围内的影响和风险,并确定适应性解决方案,对未来构成重大挑战。
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引用次数: 0
Analytical Formula for Conditional Moments of Extended Heston-CEV Hybrid Model with Time-dependent Parameters 具有时变参数的扩展Heston-CEV混合模型条件矩的解析公式
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.022
Promsiri Anunak, P. Boonserm, Udomsak Rakwongwan
This paper proposes an analytical formula for conditional moments of the extended Heston- CEV hybrid model, where the underlying asset dynamic is formed under the hybrid framework of a constant elasticity of variance (CEV) process that is the March–Rosenfeld process. The formula is received by successfully solving the partial differential equation corresponding to the infi nitesimal generator for two-dimensional process. The formula obtained is easy to use in practice and relatively more general compared with the currently existing results in the literature. In addition, numerical validations are conducted to illustrate the accuracy of our analytical formula by comparing the results gained from the Monte Carlo (MC) simulations.
本文提出了扩展Heston- CEV混合模型的条件矩解析公式,其中标的资产动态是在恒定方差弹性(CEV)过程即马奇-罗森菲尔德过程的混合框架下形成的。通过成功地求解二维过程的无穷微元发生器对应的偏微分方程,得到了该公式。所得公式易于实际应用,与现有文献结果相比,具有较强的通用性。此外,通过与蒙特卡罗(MC)模拟结果的比较,进行了数值验证,以说明分析公式的准确性。
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引用次数: 0
Potential Suitable Area of Invasive Species Cryptomonas sp. under Climate Change Scenarios in China Sea Areas 气候变化情景下中国海域入侵物种隐单胞菌的潜在适宜区
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.028
Ru Lan, Jing Li, Hai-fang Lin, B. Qiao, Yi Huang, Rulin Wang
Alien invasive algae along with ship ballast water have posed a serious threat to China’s marine ecological security. A clear understanding of the geographical distribution of invasive species and their response to climate change can provide a scientifi c basis for their prevention and control. In this study, combined with environmental variables and distribution data, MaxEnt was used to predict the potential geographical distribution and change trend of Cryptomonas sp. in the four major sea areas of China in current, 2040s (2031-2050) and 2090s (2081-2100), reveal the dominant environmental factors affecting the geographical distribution, analyze the migration trends of centroids the highly suitable area, and clarify the key control sea areas. Results showed that: 1) The most important environmental variable affecting the geographical distribution of Cryptomonas sp. was the Temperature. Range (bio24). 2) Under current climate condition, the suitability index of Cryptomonas sp. in the whole sea area of the Yellow Sea, the northern sea area of the East China Sea, and the corresponding sea areas of Jiaxing, Ningbo, Dongying, Weifang and Zhangzhou, and special attention should be paid to the prevention and control of Cryptomonas sp.. 3) Under the scenarios in 2040s (2031-2050) and 2090s (2081-2100), the total suitable area and highly suitable areas of Cryptomonas sp. in the four sea areas of China showed an increasing trend. 4) Under climate change scenarios in 2040s and 2090s, in the Yellow Sea, the centroid of the highly suitable area of Cryptomonas sp. would generally move to the southeast or southwest. In the Bohai Sea and the East China Sea, the centroid showed a trend of moving to different latitudes and directions under different scenarios. In the Bohai Sea, the centroid showed a trend of moving to the north. These moving trends indicated that Cryptomonas sp. may adapt to climate change by changingtheir distribution areas.
外来入侵藻类与船舶压载水一起对中国海洋生态安全构成严重威胁。了解入侵物种的地理分布及其对气候变化的响应,可以为入侵物种的防治提供科学依据。本研究结合环境变量和分布数据,利用MaxEnt预测了中国4个主要海域隐单胞菌在当前、2040年代(2031-2050年)和2090年代(2081-2100年)的潜在地理分布和变化趋势,揭示了影响地理分布的主导环境因素,分析了高度适宜区质心的迁移趋势,明确了重点控制海域。结果表明:1)气温是影响隐单胞菌地理分布最重要的环境变量。范围(bio24)。2)在当前气候条件下,黄海整个海域、东海北部海域以及嘉兴、宁波、东营、潍坊、漳州等相应海域隐单胞菌适宜性指数,应特别注意隐单胞菌的防治。3)在2040年代(2031-2050年)和2090年代(2081-2100年)情景下,中国4个海域隐单胞菌的总适宜面积和高度适宜面积均呈增加趋势。④在2040 ~ 2090年气候变化情景下,黄海隐单胞菌高度适宜区质心总体上向东南或西南方向移动。在渤海和东海,在不同情景下,质心呈现出向不同纬度和方向移动的趋势。在渤海,质心呈北移趋势。这些变化趋势表明,隐单胞菌可能通过改变其分布区域来适应气候变化。
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引用次数: 0
Addition to Erysiphe quercicola, A Powdery Mildew Fungus on Urena lobata in South China 中国南方白粉病真菌白粉病的研究
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.030
Shiqing Ma, Biao Xu, I. Senanayake, Jia Song
Leaves of Caesar weed (Urena lobata) with typical powdery mildew symptoms were collected from Baiyun Mountain Park in Guangzhou, China during winter and summer in 2020 and 2021. Based on the morphology and molecular sequence analyses of Internal Transcribed Spacer (ITS) and large subunit (LSU) rDNA region, the causative agent was identified as Erysiphe quercicola. A description and relevant microscopic images of the pathogen are provided. According to our knowledge, this is the first report of E. quercicola on Urena lobata and this study expands the host range of E. quercicola. Further, we provided details of life cycle and geographical distribution of the fungus.
本研究于2020年和2021年冬夏在广州白云山公园采集具有典型白粉病症状的凯撒草叶片。根据内部转录间隔区(ITS)和rDNA大亚基区(LSU)的形态和分子序列分析,确定病原菌为槲皮Erysiphe quercicola。提供了病原体的描述和相关显微图像。据我们所知,这是首次报道槲寄生寄生在白叶百合上,扩大了槲寄生的寄主范围。此外,我们还提供了真菌的生命周期和地理分布的细节。
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引用次数: 0
Extraction and Characterization of β-Glucan from Kombucha Bacterial Cellulose and Concentrated Kombucha: Evaluating their Bile Acid Binding Capacity 康普茶细菌纤维素和浓缩康普茶中β-葡聚糖的提取与表征:评价其胆汁酸结合能力
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.033
Sonthirat Charoenrak, M. Wakayama, S. Charumanee, P. Sirisa-ard, S. Bovonsombut, Suwalee Kiatkarun, T. Chitov, S. Bovonsombut
β-glucan is a water-soluble polysaccharide present in natural products and has many health benefi ts such as its role as a dietary fi ber. This study aimed to extract and characterize β-glucan in kombucha bacterial cellulose (KBC) powder and concentrated kombucha. The extraction method used was ultrasound sonication combined with enzymatic treatment. The results showed that the content of β-glucan was 19.70% (w/w) in KBC extract and 8.34% (w/v) in concentrated kombucha extract, determined using β-glucan assay kit (Mixed linkage). Fourier-transform infrared (FTIR) spectroscopy analysis confi rmed the presence of β-glucan with β-(1→3) and β-(1→4) glucan linkages, as well as the presence of proteins, mannans, and other components in both KBC β-glucan and concentrated kombucha β-glucan extracts. Regarding bile acid binding potential, concentrated kombucha β-glucan exhibited a high bile acid binding capacity for up to 24 hours, while KBC β-glucan only retained this capacity for up to 4 hours. Altogether, these fi ndings highlight the presence and properties of β-glucan in kombucha, suggesting its potential as a functional ingredient with health benefi ts.
β-葡聚糖是一种水溶性多糖,存在于天然产品中,具有许多健康益处,例如它作为膳食纤维的作用。本研究旨在对康普茶细菌纤维素(KBC)粉末和浓缩康普茶中β-葡聚糖进行提取和表征。采用超声超声联合酶法提取。结果表明:用β-葡聚糖测定试剂盒(混合连锁)测定,KBC提取物中β-葡聚糖含量为19.70% (w/w),浓缩康普茶提取物中β-葡聚糖含量为8.34% (w/v)。傅里叶变换红外(FTIR)光谱分析证实了β-葡聚糖具有β-(1→3)和β-(1→4)键,并且在KBC β-葡聚糖和浓缩康普茶β-葡聚糖提取物中存在蛋白质、甘露聚糖和其他成分。在胆汁酸结合电位方面,浓缩的康普茶β-葡聚糖表现出高达24小时的高胆汁酸结合能力,而KBC β-葡聚糖仅保持这种能力长达4小时。总之,这些发现强调了康普茶中β-葡聚糖的存在和特性,表明它可能是一种具有健康益处的功能成分。
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引用次数: 0
Mathematical Modeling of Antibiotic Resistance in Hospital with Dysbiosis 医院菌群失调患者抗生素耐药性的数学建模
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.027
Luzz Claire P. Llenos, Ivy Lorelei G. Miranda, A. A. Domogo, J. Collera
In this paper, we propose and study a transmission model among individuals in a hospital of antibiotic-resistant bacteria considering dysbiosis. The transmission of such bacteria in a hospital setting has been the focus of several previous researches. However, the aspect and contribution of antibioticinduced dysbiosis was not considered in the existing literature. Antibiotics impact the human intestinal microbiome for it unintentionally affects the needed gut microbiota diversity which are fundamental drivers of health and disease in humans. This unintentional destabilization of the healthy human microbiome results in microbial imbalance called dysbiosis. The goal of this paper is to analyze the dynamics of the proposed model in order to come up with insights and possible strategies to control antibiotic-resistant bacterial transmission among individuals in a hospital considering dysbiosis. Possible equilibria of the model system include the resistance-free equilibrium and the endemic equilibrium. The stability of the former means that the antibiotic resistance dies out, while the stability of the latter implies that the antibiotic resistance persists. We determined using sensitivity analysis that the most infl uential parameter is the drug 1 treatment rate. Moreover, we found a threshold value for this parameter, using numerical continuation, where the antibiotic resistance persists. These results provide insights on how to strategize to control the transmission of antibiotic-resistant bacteria in this setting.
在本文中,我们提出并研究了一种考虑生态失调的耐药细菌在医院个体之间的传播模型。这种细菌在医院环境中的传播一直是以前几项研究的重点。然而,现有文献没有考虑抗生素引起的生态失调的方面和贡献。抗生素影响人类肠道微生物群,因为它无意中影响了人类健康和疾病的基本驱动因素所需的肠道微生物群多样性。这种对健康人体微生物群的无意破坏导致微生物失衡,称为生态失调。本文的目的是分析所提出的模型的动力学,以便提出见解和可能的策略来控制医院中考虑生态失调的个体之间的抗生素耐药细菌传播。模型系统可能的平衡包括无阻力平衡和地方性平衡。前者的稳定性意味着抗生素耐药性的消失,而后者的稳定性意味着抗生素耐药性的持续存在。我们通过敏感性分析确定影响最大的参数是药物1治疗率。此外,我们发现了这个参数的阈值,使用数值延拓,其中抗生素耐药性持续存在。这些结果为在这种情况下如何制定控制抗生素耐药细菌传播的策略提供了见解。
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引用次数: 0
Isolation and Identifi cation of Postharvest Fungal Pathogens of Some Selected Fruits in Yunnan, China 云南部分水果采后真菌病原菌的分离鉴定
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.023
Cheng Dao, S. Karunarathna, A. H. Ekanayaka, Li Lu, Er-Fu Yang, S. Tibpromma, Kai Yan
Fruits are one of the major economic commodities worldwide and postharvest rot diseases is one of the major problems that led to decreasing fruit production. Fungal contaminants reduce the quality of fresh fruits after harvest and it is one of the leading causes of economic loss in global fresh fruits. The purpose of this research was to isolate and identify pathogens causing postharvest rots on common fruits available in markets in Panlong District, Kunming, Yunnan Province, China. The fruits viz. apple (Malus domestica), dragon fruit (Hylocereus undatus), peach (Amygdalus persica) and pear (Pyrus sorotina) were selected to examine post-harvest rots since they are commonly available fruits in Yunnan markets. We isolated six fungal strains from the above-mentioned fruits. The pathogenicity tests and phylogenetic analyses were carried out for the fungi isolated from fruits. Our results showed that Fusarium spp. are common on apple, peach and dragon fruit. In the pathogenicity test, Monilinia yunnanensis inoculated on the pear had the strongest effect on the pear, and the disease spot expanded over half of the total area of the fruit. Our results are expected to provide a reference and basis for preventing postharvest diseases of common fruits in Yunnan.
水果是世界上主要的经济商品之一,采后腐病是导致水果产量下降的主要问题之一。真菌污染降低了新鲜水果收获后的质量,是全球新鲜水果经济损失的主要原因之一。本研究旨在分离和鉴定云南省昆明市盘龙区市场上常见水果采后腐烂的病原菌。选择苹果(Malus domestica)、火龙果(Hylocereus undatus)、桃(Amygdalus persica)和梨(Pyrus sorotina)等水果进行采收后腐烂检查,因为它们是云南市场上常见的水果。从上述果实中分离到6株真菌。对从果实中分离的真菌进行了致病性试验和系统发育分析。结果表明,苹果、桃、火龙果中常见镰刀菌。在致病性试验中,接种在梨上的云南念珠菌对梨的影响最大,病斑扩大到果实总面积的一半以上。研究结果可为云南常见水果采后病害防治提供参考和依据。
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引用次数: 1
Biosurfactant-Producing Bacteria and Microbial Analysis in Mae Soon Reservoir, Fang Oil Field, Chiang Mai, Thailand 泰国清迈芳油田Mae Soon油藏生物表面活性剂产菌及微生物分析
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.024
Ladapa Kumdhitiahutsawakul, T. Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Y. Tragoolpua, S. Bovonsombut
Microbial Enhanced Oil Recovery (MEOR) is an effective alternative method for oil recovery in reservoirs using microorganisms or their secondary metabolites. This research aimed to evaluate the indigenous bacteria from Mae Soon reservoir by culture-dependent and culture-independent methods and to investigate the potential of biosurfactant-producing bacteria using a drop-collapsed assay. Indigenous bacteria were isolated from the oil sands of the reserved core of Mae Soon reservoir using fi ve different media (nutrient, Luria-Bertani, mineral salt, tryptic soy, and peptone yeast extract). Fifty-four facultative anaerobic bacterial isolates were obtained. Seven isolates showed their potential as biosurfactant producers in the drop-collapse assay. Based on 16S rRNA gene analysis, six of the biosurfactant-producing bacterial isolates belonged to the species Bacillus licheniformis and one belonged to the species B. subtilis. The biosurfactant producers and microbial community in the oil sands were determined using Denaturing Gradient Gel Electrophoresis (DGGE). Interestingly, DGGE bands corresponding to bacteria belonging to the genus Geobacillus sp. were detected. Overall, the results obtained from this work showed that indigenous bacteria in Mae Soon reservoir oil well were prospective for use in MEOR.
微生物提高采油(MEOR)是利用微生物或其次生代谢产物进行油藏采油的一种有效替代方法。本研究旨在通过培养依赖型和非培养依赖型两种方法对Mae Soon水库中的本地细菌进行评价,并利用滴落法研究产生生物表面活性剂的细菌的潜力。采用5种培养基(营养物、Luria-Bertani、无矿盐、色氨酸大豆和蛋白胨酵母提取物)从美顺储层保留岩心的油砂中分离出原生细菌。分离得到54株兼性厌氧细菌。7株分离菌在滴崩试验中显示出作为生物表面活性剂的生产潜力。根据16S rRNA基因分析,6株产生物表面活性剂的分离菌属于地衣芽孢杆菌,1株属于枯草芽孢杆菌。采用变性梯度凝胶电泳(DGGE)技术对油砂中生物表面活性剂的生成物和微生物群落进行了测定。有趣的是,检测到Geobacillus sp.属细菌对应的DGGE条带。综上所述,本研究结果表明,Mae Soon油藏油井中的原生细菌具有较好的MEOR应用前景。
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引用次数: 0
Screening of Cellulose and Pectin Degrading Actinomycetes and Evaluation of the Ability of Composite Flora to Degrade Corn Straw 纤维素和果胶降解放线菌的筛选及复合菌群降解玉米秸秆能力的评价
IF 0.4 4区 综合性期刊 Q3 Mathematics Pub Date : 2023-05-31 DOI: 10.12982/cmjs.2023.021
Xiaofei Liu, J. Lian, Zhihui Zheng, Ye Qi, Guangjian Zhang, Wei Wang, Naipu Zhang
Corn straw is a type of high-yield agricultural waste in China, but it has not been fully utilized owing to the diffi culty of degrading it and its low utilization rate. To improve the rate of degradation of corn straw and ensure that the straw resources are utilized more fully, we obtained an actinomycete that could simultaneously degrade cellulose and pectin from the cold region and black glebe of northeast China. The strain was named DPA-3-18, and its biological morphology and the sequence of its 16S rRNA were identifi ed. These analyses determined that it is a species of Streptomyces. The strain was combined with the lab-stored GS-30 and Bacillus subtilis to construct composite fl ora and conduct a single factor experiment. Signifi cantly higher factors were selected by Plackett Burman, and its fermentation conditions were optimized using response surface methodology. The optimal cultivation conditions were 4% of inoculum, a culture temperature of 28.51°C, an initial pH value of the medium of 7.43, and the oscillation during fermentation took place at 199.62 rpm. Under these conditions, 40.38% of the corn straw was degraded. F rom the results of SEM, we found that the structure of corn straw before degradation was complete, and the internal structure of corn straw after degradation with c omposite fl ora was destructed. The FT-IR spectra shown that the absorption peaks in all places of corn straw were reduced after degradation by the composite fl ora, the absorption peaks at 1640 cm-1 of corn straw after degradation were lower than that of before degrad ation, which confi rmed the decrease in cellulose. It indicated that the corn straw was signifi cantly degraded by the composite fl ora. This study contributed to understanding the change in corn straw after fermentation by the composite fl ora, and providing a theoretical basis for comprehensive utilization of corn straw.
玉米秸秆在中国是一种高产农业废弃物,但由于其降解难度大,利用率低,尚未得到充分利用。为了提高玉米秸秆的降解速度,保证秸秆资源的充分利用,我们获得了一种能同时降解东北寒区和黑地纤维素和果胶的放线菌。该菌株被命名为DPA-3-18,对其生物学形态和16S rRNA序列进行了鉴定,确定其为链霉菌属。将该菌株与实验室保存的GS-30和枯草芽孢杆菌联合构建复合菌群并进行单因素实验。通过Plackett Burman筛选出较高的影响因子,并利用响应面法对其发酵条件进行优化。最佳培养条件为接种量为4%,培养温度为28.51℃,培养基初始pH值为7.43,发酵过程中振荡发生在199.62 rpm。在此条件下,玉米秸秆的降解率为40.38%。从SEM的结果可以看出,降解前的玉米秸秆结构是完整的,而c复合菌降解后的玉米秸秆内部结构被破坏。FT-IR光谱显示,复合溶剂降解后玉米秸秆各部位的吸收峰均降低,降解后玉米秸秆在1640 cm-1处的吸收峰低于降解前,证实了纤维素的减少。结果表明,复合菌群对玉米秸秆降解效果显著。本研究有助于了解复合菌落发酵后玉米秸秆的变化,为玉米秸秆的综合利用提供理论依据。
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引用次数: 0
期刊
Chiang Mai Journal of Science
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