Pub Date : 2012-10-01DOI: 10.4103/2229-5186.103098
N. Nagdeo, N. Kaore, V. R. Thombare
Background: Many clinical laboratories have problems detecting various β-lactamases. Confusion exists about the importance of these resistance mechanisms, optimal test methods, and appropriate reporting conventions. It is more imperative to use various phenotypic methods for detection of various β-lactamases in routine microbiology laboratory on day-to-day basis to prevent antimicrobial resistance by evidence-based judicious use of antimicrobials. Aims: In view of the multidrug-resistant organisms being reported world over, we planned a cross-sectional prospective analytical study to determine resistance mechanism by various β-lactamases in Gram-negative clinical isolates using various phenotypic methods. Materials and Methods: All nonrepeat, nonenteric clinical isolates of Gram-negative bacilli, resistant to at least two third-generation cephalosporins, were first screened by Novel disc placement method, and isolates showing multiple mechanisms of resistance and reduced zone of inhibition for imipenem were further confirmed for AmpC and metallo β-lactamases. Statistical Analysis: All the data was managed and analyzed in Microsoft Excel. Results: Out of 807 isolates tested, as many as 795 (98.51%) revealed the presence of extended-spectrum β-lactamases (ESBLs). Only 10 isolates of Escherichia coli and 2 of Klebsiella pneumoniae did not show production of ESBL. A total of 450 (55.76%) isolates produced single enzyme,while 345 (42.75%) strains revealed multiple enzyme production simultaneously. Only ESBL production was seen in 315 (39.03%) strains, only AmpC in 75 (9.29%) and only MBL in 60 (7.44%) strains, while ESBL and AmpC together were seen in 219 (27.14%) and AmpC plus MBL in 92 (11.40%) strains. However, ESBL plus MBL were never observed together. All three enzymes were simultaneously detected in 34 (4.21%) strains. Conclusion: This innovative method of disc placement makes it easy, affordable, and reliable method for routine use by basic microbiology laboratories for detection of various β-lactamases, pending confirmation for AmpC and metallo β-lactamase by three-dimensional test and double disc potentiation test, respectively.
{"title":"Phenotypic methods for detection of various β-lactamases in Gram-negative clinical isolates: Need of the hour","authors":"N. Nagdeo, N. Kaore, V. R. Thombare","doi":"10.4103/2229-5186.103098","DOIUrl":"https://doi.org/10.4103/2229-5186.103098","url":null,"abstract":"Background: Many clinical laboratories have problems detecting various β-lactamases. Confusion exists about the importance of these resistance mechanisms, optimal test methods, and appropriate reporting conventions. It is more imperative to use various phenotypic methods for detection of various β-lactamases in routine microbiology laboratory on day-to-day basis to prevent antimicrobial resistance by evidence-based judicious use of antimicrobials. Aims: In view of the multidrug-resistant organisms being reported world over, we planned a cross-sectional prospective analytical study to determine resistance mechanism by various β-lactamases in Gram-negative clinical isolates using various phenotypic methods. Materials and Methods: All nonrepeat, nonenteric clinical isolates of Gram-negative bacilli, resistant to at least two third-generation cephalosporins, were first screened by Novel disc placement method, and isolates showing multiple mechanisms of resistance and reduced zone of inhibition for imipenem were further confirmed for AmpC and metallo β-lactamases. Statistical Analysis: All the data was managed and analyzed in Microsoft Excel. Results: Out of 807 isolates tested, as many as 795 (98.51%) revealed the presence of extended-spectrum β-lactamases (ESBLs). Only 10 isolates of Escherichia coli and 2 of Klebsiella pneumoniae did not show production of ESBL. A total of 450 (55.76%) isolates produced single enzyme,while 345 (42.75%) strains revealed multiple enzyme production simultaneously. Only ESBL production was seen in 315 (39.03%) strains, only AmpC in 75 (9.29%) and only MBL in 60 (7.44%) strains, while ESBL and AmpC together were seen in 219 (27.14%) and AmpC plus MBL in 92 (11.40%) strains. However, ESBL plus MBL were never observed together. All three enzymes were simultaneously detected in 34 (4.21%) strains. Conclusion: This innovative method of disc placement makes it easy, affordable, and reliable method for routine use by basic microbiology laboratories for detection of various β-lactamases, pending confirmation for AmpC and metallo β-lactamase by three-dimensional test and double disc potentiation test, respectively.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85224936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-10-01DOI: 10.4103/2229-5186.103096
F. M. Patel, J. Dave, P. J. Vyas, C. Patel
Background: A fixed dose combination of cefuroxime sodium (β lactam antibiotic) and sulbactam sodium (β Lactamase inhibitor) is used in ratio of 2:1 as powder for injection for the treatment of resistant lower respiratory tract and other infections. Aims: A simple, precise, and accurate ion-pair reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for determination of cefuroxime Na(CEF) and sulbactam Na(SUL) in injection. Materials and Methods: Isocratic RP-HPLC separation was achieved on an ACE C 18 column (150×4.6 mm id, 5 μm particle size) using the mobile phase 0.002 M tetrabutylammonium hydroxide sulfate (TBAH) in 10 mm potassium di-hydrogen phosphate buffer-acetonitrile (86:14 v/v, pH 3.7) at a flow rate of 1.0 ml/min. Results and Conclusion: The retention time of sulbactam Na and cefuroxime Na were 3.2 min and 10.2 min, respectively. The ion-pairing reagent improved the retention of highly polar sulbactam Na on reverse-phase column. The detection was performed at 210 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability, and specificity. The method was validated for linearity, precision, accuracy, robustness, solution stability, and specificity. The method was linear in the concentration range of 10-100 μg/ml for cefuroxime Na and 5-50 μg/ml for sulbactam Na, with a correlation coefficient of 0.9999 and 0.9998 for the respective drugs. The intraday precision was 0.13-0.21% and 0.48-0.65%, and the interday precision was 0.32-0.81% and 0.60-0.83% for cefuroxime Na and sulbactam Na, respectively. The accuracy (recovery) was found to be in the range of 98.76-100.61% and 98.99-100.30% for cefuroxime Na and sulbactam Na, respectively. The drugs were found to degrade under hydrolytic and oxidative conditions. The drugs could be effectively separated from different degradation products, and hence the method can be used for stability analysis.
{"title":"A validated stability indicating high-performance liquid chromatographic method for simultaneous estimation of cefuroxime sodium and sulbactam sodium in injection dosage form","authors":"F. M. Patel, J. Dave, P. J. Vyas, C. Patel","doi":"10.4103/2229-5186.103096","DOIUrl":"https://doi.org/10.4103/2229-5186.103096","url":null,"abstract":"Background: A fixed dose combination of cefuroxime sodium (β lactam antibiotic) and sulbactam sodium (β Lactamase inhibitor) is used in ratio of 2:1 as powder for injection for the treatment of resistant lower respiratory tract and other infections. Aims: A simple, precise, and accurate ion-pair reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for determination of cefuroxime Na(CEF) and sulbactam Na(SUL) in injection. Materials and Methods: Isocratic RP-HPLC separation was achieved on an ACE C 18 column (150×4.6 mm id, 5 μm particle size) using the mobile phase 0.002 M tetrabutylammonium hydroxide sulfate (TBAH) in 10 mm potassium di-hydrogen phosphate buffer-acetonitrile (86:14 v/v, pH 3.7) at a flow rate of 1.0 ml/min. Results and Conclusion: The retention time of sulbactam Na and cefuroxime Na were 3.2 min and 10.2 min, respectively. The ion-pairing reagent improved the retention of highly polar sulbactam Na on reverse-phase column. The detection was performed at 210 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability, and specificity. The method was validated for linearity, precision, accuracy, robustness, solution stability, and specificity. The method was linear in the concentration range of 10-100 μg/ml for cefuroxime Na and 5-50 μg/ml for sulbactam Na, with a correlation coefficient of 0.9999 and 0.9998 for the respective drugs. The intraday precision was 0.13-0.21% and 0.48-0.65%, and the interday precision was 0.32-0.81% and 0.60-0.83% for cefuroxime Na and sulbactam Na, respectively. The accuracy (recovery) was found to be in the range of 98.76-100.61% and 98.99-100.30% for cefuroxime Na and sulbactam Na, respectively. The drugs were found to degrade under hydrolytic and oxidative conditions. The drugs could be effectively separated from different degradation products, and hence the method can be used for stability analysis.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88545534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The semi-climbing sub-shrub Plumbago zeylanica (family: Plumbaginaceae) is a widely accepted ethnomedicine around the world including India, Pakistan, Bangladesh, Sri Lanka, and Australia. The plant is credited with potential therapeutic properties including antiatherogenic, cardiotonic, hepatoprotective, and neuroprotective properties. The present review highlights the various medicinal and pharmacological aspects along with recent updates on phytochemical contents of the plant.
{"title":"An account of phytochemicals from Plumbago zeylanica (Family: Plumbaginaceae): A natural gift to human being #","authors":"N. Kishore, B. B. Mishra, V. Tiwari, V. Tripathi","doi":"10.4103/2229-5186.99564","DOIUrl":"https://doi.org/10.4103/2229-5186.99564","url":null,"abstract":"The semi-climbing sub-shrub Plumbago zeylanica (family: Plumbaginaceae) is a widely accepted ethnomedicine around the world including India, Pakistan, Bangladesh, Sri Lanka, and Australia. The plant is credited with potential therapeutic properties including antiatherogenic, cardiotonic, hepatoprotective, and neuroprotective properties. The present review highlights the various medicinal and pharmacological aspects along with recent updates on phytochemical contents of the plant.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74426976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim : The present investigation evaluated the antimicrobial potential of methanolic extract of Citrus sinensis Linn. (Rutaceae) fruit peel. There is a basis for the traditional use of this plant for local health remedies. Materials and Methods: The antimicrobial activity of methanolic extract of C. sinensis fruit peel was tested against three bacterial and two fungal strains. Turbidimetric or tube dilution method and paper disc diffusion method were followed. Results are expressed as mean ± standard deviation. Results: The C. sinensis fruit peel methanolic extract exhibited antibacterial activity against Escherichia coli with minimum inhibitory concentration of 0.78 μg/ ml and minimum bactericidal concentration of 6.25 μg/ml, and appreciable antifungal activity with minimum inhibitory concentration of 12.5 μg/ml. The phytochemistry of C. sinensis fruit peel methanolic extract revealed the presence of carbohydrates (reducing sugars, hexose sugars, non-reducing polysaccharides, gums, and mucilages), flavonoid glycosides, coumarin glycosides, volatile oils, organic acids, fats and fixed oils. Conclusion: Most of the organic chemical constituents reported are aromatic phenolic compounds, which are known for their wide spectra of antimicrobial activity. Therefore, the bacteriostatic and fungistatic action of the tested extract may be attributed to the presence of polyphenolic compounds. In short, C. sinensis fruit peel methanolic extract is a potential source of natural antimicrobials.
{"title":"In vitro antimicrobial status of methanolic extract of Citrus sinensis Linn. fruit peel","authors":"A. Dhiman, A. Nanda, Sayeed Ahmad, B. Narasimhan","doi":"10.4103/2229-5186.99573","DOIUrl":"https://doi.org/10.4103/2229-5186.99573","url":null,"abstract":"Aim : The present investigation evaluated the antimicrobial potential of methanolic extract of Citrus sinensis Linn. (Rutaceae) fruit peel. There is a basis for the traditional use of this plant for local health remedies. Materials and Methods: The antimicrobial activity of methanolic extract of C. sinensis fruit peel was tested against three bacterial and two fungal strains. Turbidimetric or tube dilution method and paper disc diffusion method were followed. Results are expressed as mean ± standard deviation. Results: The C. sinensis fruit peel methanolic extract exhibited antibacterial activity against Escherichia coli with minimum inhibitory concentration of 0.78 μg/ ml and minimum bactericidal concentration of 6.25 μg/ml, and appreciable antifungal activity with minimum inhibitory concentration of 12.5 μg/ml. The phytochemistry of C. sinensis fruit peel methanolic extract revealed the presence of carbohydrates (reducing sugars, hexose sugars, non-reducing polysaccharides, gums, and mucilages), flavonoid glycosides, coumarin glycosides, volatile oils, organic acids, fats and fixed oils. Conclusion: Most of the organic chemical constituents reported are aromatic phenolic compounds, which are known for their wide spectra of antimicrobial activity. Therefore, the bacteriostatic and fungistatic action of the tested extract may be attributed to the presence of polyphenolic compounds. In short, C. sinensis fruit peel methanolic extract is a potential source of natural antimicrobials.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82403413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The aim of this work was a comparative research of trace element structure of various organs of three Pulmonaria species. Materials and Methods: The aerial parts of the most widespread plants of genus Pulmonaria such as Pulmonaria officinalis L., Pulmonaria obscura Dumort. and Pulmonaria mollis Wulf. ex Hornem., which were collected in ending of flowering and were used as the research objects. The amount of trace elements (B, K, P, V, Ca, Co, Cu, Fe, Mg, Mn, Mo, Na, Si, Zn, Ag, Al, Ba, Br, Cr, I, Ni, Se, Sr, and Ti) was determined by means of mass spectroscopy with inductively coupled plasma. Results: The data clustering has shown that floral shoots and rosellate leaves possess essentially various trace element status. At the same time, the trace elements' status of organs of researched plants poorly depends on a taxonomic position of the plant. Thereupon, it is obvious that pharmacological activity is defined by organs of plants from which medicines were made, but not by a species of the used plant. Conclusions: The significant distinction in pharmacological activity of preparations depends on the trace elements' status of used medicinal vegetative raw materials.
{"title":"Trace element structure of the most widespread plants of genus Pulmonaria","authors":"D. Kruglov","doi":"10.4103/2229-5186.99587","DOIUrl":"https://doi.org/10.4103/2229-5186.99587","url":null,"abstract":"Aim: The aim of this work was a comparative research of trace element structure of various organs of three Pulmonaria species. Materials and Methods: The aerial parts of the most widespread plants of genus Pulmonaria such as Pulmonaria officinalis L., Pulmonaria obscura Dumort. and Pulmonaria mollis Wulf. ex Hornem., which were collected in ending of flowering and were used as the research objects. The amount of trace elements (B, K, P, V, Ca, Co, Cu, Fe, Mg, Mn, Mo, Na, Si, Zn, Ag, Al, Ba, Br, Cr, I, Ni, Se, Sr, and Ti) was determined by means of mass spectroscopy with inductively coupled plasma. Results: The data clustering has shown that floral shoots and rosellate leaves possess essentially various trace element status. At the same time, the trace elements' status of organs of researched plants poorly depends on a taxonomic position of the plant. Thereupon, it is obvious that pharmacological activity is defined by organs of plants from which medicines were made, but not by a species of the used plant. Conclusions: The significant distinction in pharmacological activity of preparations depends on the trace elements' status of used medicinal vegetative raw materials.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89786363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Chandu, Santhosh Kumar, C. Bhattacharjee, S. Debnath
Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using "Trypan blue exclusion assay" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract) and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by "Trypan blue exclusion assay". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.
{"title":"Cytotoxicity study of plant Aloe vera (Linn)","authors":"A. Chandu, Santhosh Kumar, C. Bhattacharjee, S. Debnath","doi":"10.4103/2229-5186.99595","DOIUrl":"https://doi.org/10.4103/2229-5186.99595","url":null,"abstract":"Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using \"Trypan blue exclusion assay\" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract) and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by \"Trypan blue exclusion assay\". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90897923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Atheroclerosis is the major cause of morbidity and mortality world wide. The drugs used these days have some or other side effects. Aim: Therefore it is an urgent need to develop a safe and cheaper drugs for the society. We have planned this study to investigate lipid lowering and antioxidant activities of Bruguiera cylindrinca in triton and cholesterol fed rats. Materials and Methods: Different extract and fractions were collected from B. cylindrica. Two major compounds were isolated from there and have been tested for their lipid lowering activity along with increase in high density lipoprotein,cholesterol, compared with the cholesterol fed animals. Results: On chronic feeding of active fraction and pure compounds (at 100 mg / kg b.w.) for 30 days. The pure compounds isolated from the active fraction were found showing lipid lowering activity similar to the fraction. Conclusion: Structre modification of the two pure compounds isolated from the active fraction is required for further enhacement of biological activity.
背景:动脉粥样硬化是世界范围内发病率和死亡率的主要原因。现在使用的药物都有这样或那样的副作用。目的:因此，开发一种安全、廉价的药物是社会迫切需要的。本实验旨在探讨白茅对高脂和高胆固醇大鼠的降脂和抗氧化活性。材料与方法:从白茅中提取不同部位的提取物。从那里分离出两种主要化合物，并对它们的降脂活性进行了测试，同时与喂食胆固醇的动物相比，它们的高密度脂蛋白和胆固醇也有所增加。结果:活性部分和纯化合物(剂量为100 mg / kg b.w)长期饲喂30 d。从活性部位分离得到的纯化合物具有与该部位相似的降脂活性。结论:从活性部位分离得到的两种纯化化合物需要进行结构修饰以进一步提高生物活性。
{"title":"Antihyperlipidemic and antioxidant activities of Bruguiera cylindrinca (L)","authors":"V. Lakshmi, Ravi Sonkar, A. Khanna","doi":"10.4103/2229-5186.99596","DOIUrl":"https://doi.org/10.4103/2229-5186.99596","url":null,"abstract":"Background: Atheroclerosis is the major cause of morbidity and mortality world wide. The drugs used these days have some or other side effects. Aim: Therefore it is an urgent need to develop a safe and cheaper drugs for the society. We have planned this study to investigate lipid lowering and antioxidant activities of Bruguiera cylindrinca in triton and cholesterol fed rats. Materials and Methods: Different extract and fractions were collected from B. cylindrica. Two major compounds were isolated from there and have been tested for their lipid lowering activity along with increase in high density lipoprotein,cholesterol, compared with the cholesterol fed animals. Results: On chronic feeding of active fraction and pure compounds (at 100 mg / kg b.w.) for 30 days. The pure compounds isolated from the active fraction were found showing lipid lowering activity similar to the fraction. Conclusion: Structre modification of the two pure compounds isolated from the active fraction is required for further enhacement of biological activity.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74307371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Since drugs used these days to lower the lipids are all synthetic drugs, they have some or the other side effects, therefore in search of cheaper lipid-lowering drugs with no side effects, we have conducted a study on Chlorophytum nimonii for its lipid-lowering and antioxidant properties. Materials and Methods: Chloragin and Gemfibrozil both caused a significant decrease in the serum level of lipids in triton-induced hyperlipidemic rats, and this model has been successfully used for the evaluation of lipid-lowering activity of chloragin in the rats. Results and Discussion: The lipid-lowering action of steroidal saponin and chloragin of the C. nimonii has been studied in triton model (in cholesterol-fed hyperlipidemic rats) in vivo and antioxidant activity in vitro model. Serum lipids were found to be lowered by the steroidal saponin (100 mg/kg body weight) in triton WR-1339-induced hyperlipidemia. Chronic feeding of this compound (50 mg/kg) in animals simultaneously fed with high-fat diet for 30 days caused lowering in the lipid and lipoproteins levels of low-density lipoproteins in experimental animals. Conclusion: Chloragin activates lipolytic enzymes in plasma and liver. Chloragin is mediated through inhibition of hepatic lipids, increased fecal bile acid excretion, and enhanced plasma lecithin cholesterol acyl transferase activities. Chloragin from the C. nimonii showed potent antioxidant activity as well.
{"title":"Steroidal saponin from Chlorophytum nimonii (Grah) with lipid-lowering and antioxidant activity","authors":"V. Lakshmi, A. Mahdi, S. K. Agarwal, A. Khanna","doi":"10.4103/2229-5186.99592","DOIUrl":"https://doi.org/10.4103/2229-5186.99592","url":null,"abstract":"Aim: Since drugs used these days to lower the lipids are all synthetic drugs, they have some or the other side effects, therefore in search of cheaper lipid-lowering drugs with no side effects, we have conducted a study on Chlorophytum nimonii for its lipid-lowering and antioxidant properties. Materials and Methods: Chloragin and Gemfibrozil both caused a significant decrease in the serum level of lipids in triton-induced hyperlipidemic rats, and this model has been successfully used for the evaluation of lipid-lowering activity of chloragin in the rats. Results and Discussion: The lipid-lowering action of steroidal saponin and chloragin of the C. nimonii has been studied in triton model (in cholesterol-fed hyperlipidemic rats) in vivo and antioxidant activity in vitro model. Serum lipids were found to be lowered by the steroidal saponin (100 mg/kg body weight) in triton WR-1339-induced hyperlipidemia. Chronic feeding of this compound (50 mg/kg) in animals simultaneously fed with high-fat diet for 30 days caused lowering in the lipid and lipoproteins levels of low-density lipoproteins in experimental animals. Conclusion: Chloragin activates lipolytic enzymes in plasma and liver. Chloragin is mediated through inhibition of hepatic lipids, increased fecal bile acid excretion, and enhanced plasma lecithin cholesterol acyl transferase activities. Chloragin from the C. nimonii showed potent antioxidant activity as well.","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82601843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prasoon Gupta, Upasana Sharma, P. Gupta, R. Maurya
Bioassay-directed fractionation led to the identification of a new compound, 4-hydroxy-heptadec-6-enoic acid ethyl ester (1) together with three known compounds (2-4) from Tinospora sinensis. The structure of 1 was determined by analysis of spectroscopic data. The isolated compounds were evaluated for their protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. Compounds 1 and 2 displayed significant inhibitory activity with IC 50 values 61.1 and 74.2 μM, respectively
{"title":"A novel protein tyrosine phosphatase 1B inhibitor from Tinospora sinensis","authors":"Prasoon Gupta, Upasana Sharma, P. Gupta, R. Maurya","doi":"10.4103/2229-5186.99569","DOIUrl":"https://doi.org/10.4103/2229-5186.99569","url":null,"abstract":"Bioassay-directed fractionation led to the identification of a new compound, 4-hydroxy-heptadec-6-enoic acid ethyl ester (1) together with three known compounds (2-4) from Tinospora sinensis. The structure of 1 was determined by analysis of spectroscopic data. The isolated compounds were evaluated for their protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. Compounds 1 and 2 displayed significant inhibitory activity with IC 50 values 61.1 and 74.2 μM, respectively","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74064648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Special issue in honor of Dr. Norman R. Farnsworth","authors":"Prasoon Gupta","doi":"10.4103/2229-5186.99555","DOIUrl":"https://doi.org/10.4103/2229-5186.99555","url":null,"abstract":"","PeriodicalId":10187,"journal":{"name":"Chronicles of Young Scientists","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76785860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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