Clinical and Experimental Dental Research最新文献_第8页

Polycaprolactone Impregnated 3D Printed Nanohydroxyapatite for Sinus Augmentation: A Randomized Controlled Trial 聚己内酯浸渍3D打印纳米羟基磷灰石用于鼻窦增强：一项随机对照试验。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-10-07 DOI: 10.1002/cre2.70237

Poommarin Thammanoonkul, Suwit Limpattamapanee, Faungchat Thammarakcharoen, Jintamai Suwanprateeb, Hyun-Chang Lim, Borvornwut Buranawat

Objective

To compare the effect of polycaprolactone impregnated 3D printed nano-hydroxyapatite (3DPHA-PCL) with bovine bone substitute material (BB) in lateral maxillary sinus floor elevation (MSFE).

Materials and Methods

Lateral MSFE with two bone substitute materials was randomly performed in two centers: group BB (n = 11 sinuses) or group 3DPHA-PCL (n = 11 sinuses). Lateral MSFE with two bone substitute materials was performed on 21 participants across two centers, resulting in a total of 22 sinuses analyzed. The sinuses were randomly allocated into two groups: group BB (11 sinuses) and group 3DPHA-PCL (11 sinuses). Cone beam computed tomography (CBCT) was taken before (T0) and immediately after MSFE (T1), at 6 months (implant placement; T2), and 1 year (T3). Dimensional stability of the augmentation was analyzed using serial CBCT scans. At the time of implant placement, bone core biopsy was performed, followed by microcomputed tomographic (micro-CT) and histomorphometric analyses.

Results

Based on the superimposed CBCT images between T1 and T2, the augmented height and volume decreased in both groups without a statistically significant difference between the groups (−0.48 ± 1.01 mm, −53.9 ± 117.8 mm³ in group BB vs. −0.39 ± 0.44 mm, −40.8 ± 101.2 mm³ in group 3DPHA-PCL, p > 0.05). The percentage of newly formed bone was statistically significantly lower in group 3DPHA-PCL (15.7 ± 7.5% histomorphometrically, 16.7 ± 7.5% in micro-CT) than group BB (25.6 ± 7.2%, 26.3 ± 4.1%) (p < 0.05 in both methods). Two implants failed in the 3DPHA-PCL group, while no failures in the BB group.

Conclusions

Dimensional stability of the augmented bone was comparable between the groups. However, group 3DPHA-PCL demonstrated inferior new bone formation and implant survival compared to group BB. Long-term follow-up is warranted to monitor the behavior of 3DPHA-PCL.

目的：比较聚己内酯浸渍3D打印纳米羟基磷灰石（3DPHA-PCL）与牛骨替代材料（BB）在上颌侧窦底提升（MSFE）中的效果。材料和方法：在BB组（n = 11个鼻窦）和3DPHA-PCL组（n = 11个鼻窦）两个中心随机进行两种骨替代材料的侧位MSFE。在两个中心的21名参与者中进行了两种骨替代材料的横向MSFE，共分析了22个鼻窦。随机分为两组：BB组（11个）和3DPHA-PCL组（11个）。分别在术前（T0）、术后（T1）、术后6个月（T2）和术后1年（T3）进行锥形束ct检查。利用连续CBCT扫描分析增强体的尺寸稳定性。植入时进行骨芯活检，随后进行显微计算机断层扫描（micro-CT）和组织形态学分析。结果：T1与T2之间的CBCT叠加图像显示，两组患者的增高高度和体积均有所下降（BB组为-0.48±1.01 mm, -53.9±117.8 mm3, 3DPHA-PCL组为-0.39±0.44 mm, -40.8±101.2 mm3, p > 0.05），但组间差异无统计学意义。3DPHA-PCL组新生成骨百分率（组织形态学为15.7±7.5%，显微ct为16.7±7.5%）低于BB组（25.6±7.2%，显微ct为26.3±4.1%）(p)。结论：两组间增强骨的尺寸稳定性相当。然而，与BB组相比，3DPHA-PCL组的新骨形成和种植体存活率较低。需要长期随访监测3DPHA-PCL的行为。

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引用次数: 0

DNA Methylation of COX-2, IFN-γ, TNF-α, and LINE-1 in Clinically Stable Periodontal Tissues Following Periodontal Therapy 牙周治疗后临床稳定牙周组织中COX-2、IFN-γ、TNF-α和LINE-1的DNA甲基化

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-10-07 DOI: 10.1002/cre2.70229

Giulio Rasperini, Koki Yoshida, Alessandro Martinotti, Valentina Bollati, Letizia Tarantini, Farah Asa'ad

Background

Epigenetic modifications such as DNA methylation play a crucial role in the regulation of gene expression in inflammatory diseases, including periodontitis. While previous studies have examined methylation changes during active disease or shortly after treatment, little is known about the epigenetic landscape of periodontal tissues that have remained clinically stable over the long term after Supportive Periodontal Therapy (SPT).

Methods

We collected gingival tissue samples from 40 individuals, including 20 with a history of periodontitis currently under long-term SPT and 20 periodontally healthy controls. DNA methylation levels of LINE-1 (a marker of global methylation) and inflammation-related genes COX-2 (PTGS2), IFN-γ (IFNG), and TNF-α (TNF) were analyzed using bisulfite pyrosequencing.

Results

The LINE-1 methylation percentage was significantly higher in the periodontitis group than in the healthy group (66.5% ± 2.0 vs. 63.9% ± 4.0; p = 0.03). However, this significance was lost after adjusting for age and gender. No significant differences were observed between groups for COX-2, IFN-γ, or TNF-α. Genomic context analysis using the Encyclopedia of DNA Elements annotations revealed that the CpG sites analyzed for PTGS2, IFNG, and TNF are in distal regulatory regions enriched with enhancer-like elements, histone modifications, and predicted NFKB1 binding motifs.

Conclusions

These findings suggest that LINE-1 methylation in clinically stable gingival tissues may reflect long-term epigenetic memory from previous chronic inflammation. Motif-level analysis highlighted potential regulatory input from NFKB1 at the three loci (PTGS2, IFNG, and TNF). Notably, no significant epigenetic differences were observed in the inflammation-related genes COX-2, IFN-γ, and TNF-α, suggesting that periodontal disease can be effectively treated and that certain inflammatory markers may return to levels comparable to those seen in individuals who have never had the disease. These results highlight the importance of examining DNA methylation dynamics not only during active disease but also during long-term remission.

背景：表观遗传修饰如DNA甲基化在包括牙周炎在内的炎症性疾病的基因表达调控中起着至关重要的作用。虽然以前的研究已经检查了活动性疾病期间或治疗后不久的甲基化变化，但对于支持牙周治疗（SPT）后长期保持临床稳定的牙周组织的表观遗传景观知之甚少。方法：我们收集了40个人的牙龈组织样本，其中20人有牙周炎病史，目前正在接受长期SPT治疗，20人是牙周健康对照。使用亚硫酸氢盐焦磷酸测序分析LINE-1（全局甲基化标志物）和炎症相关基因COX-2 （PTGS2）、IFN-γ (IFNG)和TNF-α (TNF)的DNA甲基化水平。结果：牙周炎组LINE-1甲基化率明显高于健康组（66.5%±2.0∶63.9%±4.0;p = 0.03）。然而，在调整了年龄和性别后，这种重要性就消失了。各组间COX-2、IFN-γ或TNF-α含量无显著差异。使用DNA元件百科全书注释的基因组背景分析显示，PTGS2、IFNG和TNF分析的CpG位点位于远端调控区域，富含增强子样元件、组蛋白修饰和预测的NFKB1结合基序。结论：这些研究结果表明，临床稳定的牙龈组织中的LINE-1甲基化可能反映了以前慢性炎症的长期表观遗传记忆。基序水平分析强调了NFKB1在三个位点（PTGS2、IFNG和TNF）上的潜在调控输入。值得注意的是，在炎症相关基因COX-2、IFN-γ和TNF-α中没有观察到显著的表观遗传差异，这表明牙周病可以有效治疗，某些炎症标志物可能会恢复到与从未患过牙周病的个体相当的水平。这些结果强调了检测DNA甲基化动力学的重要性，不仅在活动性疾病期间，而且在长期缓解期间。

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引用次数: 0

Evaluating Maternal Accuracy in Smartphone-Based Tele-Dentistry for Early Childhood Caries Detection 评估母亲在基于智能手机的远程牙科早期儿童龋齿检测中的准确性。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-10-03 DOI: 10.1002/cre2.70231

Parastoo Iranparvar, Zahra Ghorbani, Sajjad Zandieh, Serlie Hartoonian, Mohsen Shirazi

Background

Early childhood caries (ECC) is a public health challenge, leading to long-term dental problems and costs. Limited access to preventive care underscores the need for innovative screening methods. Tele-dentistry, using smartphone-based imaging, offers a scalable solution for ECC detection and intervention.

Objective

This study evaluates the accuracy of maternal smartphone-based ECC assessments compared to professional evaluations as a reliable screening tool.

Methods

A cross-sectional-study recruited 114 mother–child pairs from primary healthcare centers in Tehran province, Iran. Mothers received online training and conducted visual and smartphone-based caries assessments. Two blinded pediatric dentists remotely reviewed images, with findings compared to in-person dental examinations. Sensitivity, specificity, and intraclass correlation coefficients (ICCs) were calculated.

Results

The mean child age was 4.8 ± 0.9 years with 56.1% boys and 43.9% girls. Mothers had a mean age of 33.2 ± 5.6 years with 55% having a high school diploma or higher. Maternal assessments identified caries in 49/58 cases (84.5%), while pediatric dentists detected 51/58 cases (87.9%). Maternal assessments showed 100% sensitivity and specificity, with strong agreement with professional evaluations (ICC = 0.909–0.988, p < 0.0001). The three methods demonstrated near-perfect concordance (ICC = 0.978, p < 0.0001).

Conclusion

Maternal-assisted tele-dentistry is a reliable, accessible screening method for ECC, improving early intervention, especially in low resources settings and underserved communities.

背景：儿童早期龋齿（ECC）是一项公共卫生挑战，导致长期的牙齿问题和费用。获得预防保健的机会有限，这突出表明需要创新的筛查方法。远程牙科使用基于智能手机的成像技术，为ECC检测和干预提供了可扩展的解决方案。目的：本研究评估基于智能手机的母体ECC评估与专业评估作为可靠筛查工具的准确性。方法：一项横断面研究从伊朗德黑兰省的初级卫生保健中心招募了114对母子。母亲们接受了在线培训，并进行了基于视觉和智能手机的龋齿评估。两名不知情的儿科牙医远程查看了图像，并将结果与面对面的牙科检查进行了比较。计算敏感性、特异性和类内相关系数（ICCs）。结果：患儿平均年龄为4.8±0.9岁，男童占56.1%，女童占43.9%。母亲的平均年龄为33.2±5.6岁，其中55%具有高中或更高学历。产妇评估发现49/58例（84.5%）有龋齿，而儿科牙医发现51/58例（87.9%）。结论：孕产妇辅助远程牙科是一种可靠的、可获得的ECC筛查方法，可改善早期干预，特别是在资源匮乏和服务欠缺的社区。

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引用次数: 0

Curcumin Versus Corticosteroids for Symptomatic Oral Lichen Planus: A Systematic Review and Meta-Analysis 姜黄素与皮质类固醇治疗症状性口腔扁平苔藓：系统综述和荟萃分析

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-22 DOI: 10.1002/cre2.70227

Sadeq A. Al-Maweri, Gamilah Al-Qadhi, Ahmed Yaseen Alqutaibi, Nadhem M. Sallam, Mounzer Assad, Mahfoudh A. Abdulghani, Marwan Mansoor Ali Mohammed

Objectives

The management of oral lichen planus (OLP) is challenging, with no definitive cure available. This systematic review and meta-analysis aimed to assess the efficacy of curcumin in managing OLP.

Material and Methods

A comprehensive search of PubMed, Scopus, Web of Science, and Google Scholar was conducted for relevant clinical trials published up to March 31, 2025. All clinical trials comparing the efficacy of curcumin to corticosteroids were included. A meta-analysis was conducted on studies with available numerical data to assess the efficacy of curcumin relative to the control group.

Results

Eleven clinical trials, involving 499 OLP patients, were included in this review, with nine studies were incorporated into the meta-analysis. All studies reported curcumin to be efficacious in alleviating the signs and symptoms of OLP. Pooled data showed significantly better efficacy of curcumin in reducing pain at 1 week (SMD, −0.70; 95% CI, −1.33, −0.07; p = 0.03; I² = 66%) compared with corticosteroids. However, no significant differences were observed between the two groups at the 2-, 4-, and 12-week follow-ups. Additionally, curcumin and corticosteroids demonstrated comparable efficacy in clinical improvement of lesions across different time points.

Conclusions

The available evidence suggests that curcumin has promising effects in managing OLP. However, due to methodological limitations, including significant heterogeneity among the studies and a high risk of bias in most of them, further well-designed studies with adequate follow-up periods are needed.

目的口腔扁平苔藓（OLP）的治疗具有挑战性，目前尚无明确的治疗方法。本系统综述和荟萃分析旨在评估姜黄素治疗OLP的疗效。材料与方法综合检索PubMed、Scopus、Web of Science、谷歌Scholar，检索截止2025年3月31日已发表的相关临床试验。所有比较姜黄素与皮质类固醇疗效的临床试验均被纳入。一项荟萃分析对研究进行了可用的数值数据，以评估姜黄素相对于对照组的疗效。结果本综述纳入了11项临床试验，涉及499例OLP患者，其中9项研究纳入meta分析。所有研究都报道姜黄素对缓解OLP的症状和体征有效。综合数据显示，姜黄素在1周时减轻疼痛的疗效明显优于皮质类固醇（SMD, - 0.70; 95% CI, - 1.33, - 0.07; p = 0.03; I2 = 66%）。然而，在2周、4周和12周的随访中，两组之间没有观察到显著差异。此外，姜黄素和皮质类固醇在不同时间点病变的临床改善方面表现出相当的疗效。结论姜黄素对OLP有较好的治疗作用。然而，由于方法学的局限性，包括研究之间的显著异质性和大多数研究的高偏倚风险，需要进一步精心设计的研究和足够的随访期。

{"title":"Curcumin Versus Corticosteroids for Symptomatic Oral Lichen Planus: A Systematic Review and Meta-Analysis","authors":"Sadeq A. Al-Maweri, Gamilah Al-Qadhi, Ahmed Yaseen Alqutaibi, Nadhem M. Sallam, Mounzer Assad, Mahfoudh A. Abdulghani, Marwan Mansoor Ali Mohammed","doi":"10.1002/cre2.70227","DOIUrl":"https://doi.org/10.1002/cre2.70227","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The management of oral lichen planus (OLP) is challenging, with no definitive cure available. This systematic review and meta-analysis aimed to assess the efficacy of curcumin in managing OLP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A comprehensive search of PubMed, Scopus, Web of Science, and Google Scholar was conducted for relevant clinical trials published up to March 31, 2025. All clinical trials comparing the efficacy of curcumin to corticosteroids were included. A meta-analysis was conducted on studies with available numerical data to assess the efficacy of curcumin relative to the control group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Eleven clinical trials, involving 499 OLP patients, were included in this review, with nine studies were incorporated into the meta-analysis. All studies reported curcumin to be efficacious in alleviating the signs and symptoms of OLP. Pooled data showed significantly better efficacy of curcumin in reducing pain at 1 week (SMD, −0.70; 95% CI, −1.33, −0.07; <i>p</i> = 0.03; <i>I</i><sup>2</sup> = 66%) compared with corticosteroids. However, no significant differences were observed between the two groups at the 2-, 4-, and 12-week follow-ups. Additionally, curcumin and corticosteroids demonstrated comparable efficacy in clinical improvement of lesions across different time points.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The available evidence suggests that curcumin has promising effects in managing OLP. However, due to methodological limitations, including significant heterogeneity among the studies and a high risk of bias in most of them, further well-designed studies with adequate follow-up periods are needed.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Flexural Strength and Brittleness of a Polyjet 3D-Printed Denture Base Polymer 3d打印义齿基托聚合物抗弯强度和脆性分析

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-21 DOI: 10.1002/cre2.70228

Gregory W. Bennett, Alex Kohler

Objectives

This study sought to understand the effects of print orientation on a novel 3D-printing technology recently made available for the fabrication of dentures.

Material and Methods

A total of 90 experimental samples (30 each at 0, 45, and 90 degrees) were printed using denture resin on a polyjet printer. The milled control samples (n = 10) were milled on a 5-axis dental laboratory mill from a denture base disc. 96 samples were tested for flexural strength and extension at break using a 3-point bend test on a universal testing machine. The data were analyzed using statistical software and evaluated for statistical significance using general linear models with a Dunnett test with α = 0.05.

Results

The results showed that the flexural strength and extension at break were both affected by print orientation with the 90-degree orientation being the lowest performing group for both properties. The 0-degree and 45-degree orientations both exceeded the ISO minimum flexural strength of 65 MPa, with mean flexural strengths of 88.18 and 73.53 MPa. The 90-degree group mean was well below the standard at 28.12 MPa. The milled sample group mean was 65.18 MPa. Extension at break showed similar results with less variation in the printed groups. Mean extension at break of the milled samples group was 15.05 mm, the 0-degree was 5.99 mm, 45-degree was 4.72 mm, and 90-degree was 1.92 mm.

Conclusion

Flexural strength and extension at break were influenced by print orientation. A 0-degree, or horizontal, print orientation yielded the highest values for flexural strength. The milled samples had significantly higher values for extension at break, but similar values for flexural strength.

本研究旨在了解打印方向对一种新型3d打印技术的影响，该技术最近可用于制造假牙。材料与方法使用义齿树脂在聚喷打印机上打印90个实验样品（0度、45度和90度各30个）。磨铣的对照样本（n = 10）在5轴牙科实验室铣床上从义齿基托盘上铣削。在万能试验机上采用三点弯曲试验对96个试样进行了抗弯强度和断裂伸长率的测试。采用统计学软件对资料进行分析，采用一般线性模型和Dunnett检验（α = 0.05）评价统计学显著性。结果打印方向对弯曲强度和断裂伸长率均有影响，其中90度方向对弯曲强度和断裂伸长率影响最小。0°和45°取向均超过ISO最小抗弯强度65 MPa，平均抗弯强度分别为88.18和73.53 MPa。90度组平均值远低于28.12 MPa的标准。磨样组平均为65.18 MPa。休息时伸展显示出相似的结果，在印刷组中变化较小。磨样组断裂伸长率平均为15.05 mm， 0度伸长率为5.99 mm， 45度伸长率为4.72 mm， 90度伸长率为1.92 mm。结论骨折时的屈曲强度和伸长力受打印方向的影响。0度，或水平，印刷方向产生了最高的弯曲强度值。铣削后的试样在断裂时的延伸性值明显较高，但弯曲强度值相似。

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引用次数: 0

Effects of Drug Administration on Postoperative Pain in Orthognathic Surgery: A Systematic Review 药物给药对正颌手术术后疼痛的影响：系统综述

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-21 DOI: 10.1002/cre2.70226

Gianna Dipalma, Angelo Michele Inchingolo, Lilla Riccaldo, Roberta Morolla, Pietro Lauria, Roberto Vito Giorgio, Valeria Colonna, Benito F. P. Pennacchio, Antonio Mancini, Andrea Palermo, Alessio Danilo Inchingolo, Francesco Inchingolo

Objectives

This systematic review aims to offer indications on how drug administration affects postoperative pain in patients undergoing orthognathic surgery.

Material and Methods

We searched Pubmed, Scopus, and Web of Science for articles pertaining to our research topic. The search strategy was created by combining terms associated with our review's goal, which is to ascertain whether drugs used before, during, and after surgery help lessen postoperative pain. The keywords used were "orthodontic surgery AND postoperative pain." Inclusion criteria used to select articles were human subjects only, open-access studies, clinical and randomized controlled trials, English language, and studies published no more than 10 years before the current edition. Exclusion conditions such as eta-analyses, systematic reviews, off-topics, abstracts, posters, and public trials were excluded.

Results

Thirteen randomized control trials and one comparative study comprised the design of the chosen research. The studies show that administering a certain drug in comparison to another can lessen the severity of postoperative sequelae.

Conclusions

A multimodal approach is needed to alleviate post-discomfort after orthognathic surgery. Medication given before surgery can lessen discomfort and the need for opioids. Pain intensity can be decreased, and early opioid tolerance can be avoided with intraoperative treatments. Non-steroidal anti-inflammatory drugs and iontophoresis-based transdermal medication administration are other alternatives.

目的：本系统综述旨在提供药物给药如何影响正颌手术患者术后疼痛的适应症。材料和方法我们检索了Pubmed， Scopus和Web of Science关于我们研究主题的文章。搜索策略是通过结合与我们综述目标相关的术语创建的，目的是确定手术前、手术中和手术后使用的药物是否有助于减轻术后疼痛。关键词是“正畸手术和术后疼痛”。用于选择文章的纳入标准为仅限人类受试者、开放获取研究、临床和随机对照试验、英语语言以及在当前版本之前发表不超过10年的研究。排除条件包括meta分析、系统评价、偏离主题、摘要、海报和公开试验。结果13项随机对照试验和1项比较研究组成了所选研究的设计。研究表明，与另一种药物相比，使用某种药物可以减轻术后后遗症的严重程度。结论需要多模式入路来减轻正颌手术后的不适。手术前给予的药物可以减轻不适和对阿片类药物的需求。术中治疗可降低疼痛强度，避免早期阿片类药物耐受性。非甾体抗炎药和基于离子电阻的透皮给药是其他选择。

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引用次数: 0

Low Intensity Thermal Stimulation to Enhance Early Osteointegration in Implants: A Preclinical Study in Rabbits 低强度热刺激促进兔植入物早期骨整合的临床前研究。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-19 DOI: 10.1002/cre2.70223

Zhu Xiufeng, Wang Miao, Zhou Huixia, Xu Boya, Chang Xiaofeng, He Longlong

Objectives

This study is the first to integrate 3D finite element modeling, in vitro validation, and preclinical animal experiments to determine the efficacy of low-intensity thermal stimulation (LITS) in enhancing dental implant osseointegration. The study seeks to provide experimental evidence for applying thermal stimulation as a possible approach to enhance osseointegration.

Material and Methods

A 3D finite element implant-femur model and in vitro implant-bone system were developed to simulate heat distribution. LITS conditions (50°C/5 s) were validated to avoid exceeding the osteoblast safety threshold (47°C). Eighteen rabbits received femoral implants divided into: control (no heating), T1 (single 50°C/5 s heating cycle), and T2 (three cycles). Outcomes included implant stability (IST), reverse torque, bone volume fraction (BV/TV), and histomorphometric osseointegration rate at 6 weeks.

Results

Finite element and in vitro analyses confirmed 50°C/5 s as the optimal protocol, maintaining implant surface temperatures ≤ 46.3°C and complete thermal recovery within 1 min. T1 significantly increased in vivo rabbit model reverse torque (p < 0.05) and BV/TV (p < 0.05), while T2 showed no BV/TV improvement. Both T1 and T2 exhibited higher osseointegration rates (p < 0.05). Implant stability (IST) remained unchanged across groups (p > 0.05).

Conclusion

LITS at 50°C/5 s safely enhances early osseointegration in rabbits, increasing biomechanical anchorage and peri-implant bone formation. This study provides preliminary experimental evidence for the potential of thermal application in enhancing implant osseointegration.

目的：本研究首次将三维有限元建模、体外验证和临床前动物实验相结合，以确定低强度热刺激（LITS）增强种植体骨整合的效果。本研究旨在为应用热刺激作为促进骨整合的可能方法提供实验证据。材料与方法：建立三维有限元种植体-股骨模型和体外种植体-骨系统，模拟热分布。验证了LITS条件（50°C/5 s）以避免超过成骨细胞安全阈值（47°C）。18只兔接受股骨植入物治疗，分为对照组（不加热）、T1（单次50°C/5 s加热周期）和T2（3个周期）。结果包括6周时种植体稳定性（IST）、反向扭矩、骨体积分数（BV/TV）和组织形态骨整合率。结果：有限元和体外分析证实50°C/5 s为最佳方案，维持种植体表面温度≤46.3°C，并在1 min内完全热恢复。T1显著增加了兔体内模型反向转矩（p 0.05）。结论：50°C/5 s的LITS安全增强家兔早期骨整合，增加生物力学锚定和种植体周围骨形成。本研究为热应用在增强种植体骨整合方面的潜力提供了初步的实验证据。

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引用次数: 0

Investigation of the Combined Impact of Zinc Oxide Nanoparticles and Bacterial Cellulose Nanocrystals on the Bond Strength to Dentin and Fracture Resistance of Resin-Modified Glass Ionomer Cement in Primary Molars 氧化锌纳米颗粒和细菌纤维素纳米晶体对树脂改性玻璃离聚体水门合剂与牙本质结合强度及抗断裂性能影响的研究

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-19 DOI: 10.1002/cre2.70224

Ali Nozari, Fatemeh Parvizi, Zahra Jowkar, Farnaz Haji Abbas Oghli, Hosein Askari, Seyed Ahmadreza Hamidi

Objectives

This study aimed to evaluate the microshear bond strength (µSBS) of resin-modified glass ionomer cement (RMGIC) to primary dentin and the fracture resistance of primary molars restored with RMGIC, with and without the incorporation of mesoporous zinc oxide nanoparticles (ZnO NPs) and bacterial cellulose nanocrystals (BCNCs).

Materials and Methods

A total of 100 extracted primary mandibular second molars were divided into two tests: the µSBS test (40 teeth) and the fracture resistance test (60 teeth). The µSBS test included four groups: (1) RMGIC (control), (2) RMGIC + 5 wt.% mesoporous ZnO NPs, (3) RMGIC + 1 wt.% BCNCs, and (4) RMGIC + 5 wt.% mesoporous ZnO NPs and 1 wt.% BCNCs. The fracture resistance test included these groups along with an intact teeth group (positive control) and a prepared but unrestored teeth group (negative control). A universal testing machine was used for all mechanical tests.

Results

The RMGIC + 1 wt.% BCNCs group exhibited the highest µSBS (6.35 ± 1.98 MPa), significantly surpassing the control and other experimental groups (p < 0.001). For fracture resistance, the negative control had the lowest value (422.70 ± 44.50 N, p < 0.05), while the positive control had the highest, significantly outperforming all groups except RMGIC + 1 wt.% BCNCs (p > 0.05). The RMGIC + 1 wt.% BCNCs group (1280.40 ± 340.87 N) demonstrated significantly greater fracture resistance than both RMGIC and RMGIC + 5 wt.% mesoporous ZnO (p < 0.05).

Conclusions

Incorporating 1 wt.% BCNCs into RMGIC significantly enhanced both microshear bond strength and fracture resistance, leading to a higher proportion of restorable fractures. The positive correlation between bond strength and fracture resistance suggests that BCNCs-modified RMGIC is a promising restorative material for improving durability in primary molars.

目的：研究树脂改性玻璃离子水门合剂（RMGIC）与初级牙本质的微剪切结合强度（µSBS），以及在添加介孔氧化锌纳米粒子（ZnO NPs）和细菌纤维素纳米晶体（BCNCs）和未添加介孔氧化锌纳米粒子（ZnO NPs）和细菌纤维素纳米晶体（BCNCs）的情况下，RMGIC修复初级磨牙的抗断裂能力。材料与方法：100颗拔除的下颌第二磨牙分为两组试验：µSBS试验（40牙）和抗折试验（60牙）。µSBS试验包括四组：(1)RMGIC（对照），(2)RMGIC + 5 wt。%介孔ZnO NPs， (3) RMGIC + 1 wt。% BCNCs和(4)RMGIC + 5 wt。%介孔ZnO NPs和1 wt。% BCNCs。抗折试验包括这些组以及完整牙组（阳性对照）和准备但未修复的牙组（阴性对照）。所有的机械试验都使用了万能试验机。结果：RMGIC + 1 wt。% BCNCs组的µSBS最高（6.35±1.98 MPa），显著高于对照组和其他实验组（p 0.05）。RMGIC + 1 wt。% BCNCs组（120.40±340.87 N）的抗骨折性明显高于RMGIC和RMGIC + 5。结论：加入1 wt。BCNCs加入RMGIC后，微剪切结合强度和抗断裂能力均显著增强，可修复骨折比例更高。粘结强度与断裂抗力呈正相关，表明bcncs改性RMGIC是一种很有前途的提高磨牙耐久性的修复材料。

{"title":"Investigation of the Combined Impact of Zinc Oxide Nanoparticles and Bacterial Cellulose Nanocrystals on the Bond Strength to Dentin and Fracture Resistance of Resin-Modified Glass Ionomer Cement in Primary Molars","authors":"Ali Nozari, Fatemeh Parvizi, Zahra Jowkar, Farnaz Haji Abbas Oghli, Hosein Askari, Seyed Ahmadreza Hamidi","doi":"10.1002/cre2.70224","DOIUrl":"10.1002/cre2.70224","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to evaluate the microshear bond strength (µSBS) of resin-modified glass ionomer cement (RMGIC) to primary dentin and the fracture resistance of primary molars restored with RMGIC, with and without the incorporation of mesoporous zinc oxide nanoparticles (ZnO NPs) and bacterial cellulose nanocrystals (BCNCs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 100 extracted primary mandibular second molars were divided into two tests: the µSBS test (40 teeth) and the fracture resistance test (60 teeth). The µSBS test included four groups: (1) RMGIC (control), (2) RMGIC + 5 wt.% mesoporous ZnO NPs, (3) RMGIC + 1 wt.% BCNCs, and (4) RMGIC + 5 wt.% mesoporous ZnO NPs and 1 wt.% BCNCs. The fracture resistance test included these groups along with an intact teeth group (positive control) and a prepared but unrestored teeth group (negative control). A universal testing machine was used for all mechanical tests.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The RMGIC + 1 wt.% BCNCs group exhibited the highest µSBS (6.35 ± 1.98 MPa), significantly surpassing the control and other experimental groups (<i>p</i> < 0.001). For fracture resistance, the negative control had the lowest value (422.70 ± 44.50 N, <i>p</i> < 0.05), while the positive control had the highest, significantly outperforming all groups except RMGIC + 1 wt.% BCNCs (<i>p</i> > 0.05). The RMGIC + 1 wt.% BCNCs group (1280.40 ± 340.87 N) demonstrated significantly greater fracture resistance than both RMGIC and RMGIC + 5 wt.% mesoporous ZnO (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Incorporating 1 wt.% BCNCs into RMGIC significantly enhanced both microshear bond strength and fracture resistance, leading to a higher proportion of restorable fractures. The positive correlation between bond strength and fracture resistance suggests that BCNCs-modified RMGIC is a promising restorative material for improving durability in primary molars.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Penicillin Disrupts Dental Mineralization in Rats: A Comparative Study With Tetracycline Highlighting Prenatal and Postnatal Risks 青霉素破坏大鼠牙齿矿化：与四环素突出产前和产后风险的比较研究。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-19 DOI: 10.1002/cre2.70225

Sedigheh Mozafar, Fateme Mashhadi Abbas, Majid Mehran, Somayeh Kameli, Motahare Ahmadvand, Amir Mohammad Sharafi, Reza Omid, Morteza Banakar

Objectives

Penicillin's impact on enamel defects remains understudied, particularly regarding its ability to cause structural issues even at therapeutic doses.

Material and Methods

Pregnant Wistar rats received daily gavage from gestational day 13–22 with saline (control), 130 mg/kg tetracycline, 50 mg/kg penicillin, or 100 mg/kg penicillin. After birth, pups received the same treatment for 12 days. Upper first molars were analyzed for enamel/dentin thickness, maturation, and histopathological changes.

Results

Tetracycline significantly reduced enamel and dentin thickness, disrupted their development, and caused flattening of the dentin-enamel junction (DEJ) compared to the control group. Penicillin at 100 mg/kg also significantly decreased enamel and dentin thickness, impaired their maturation, and led to DEJ flattening. At 50 mg/kg, penicillin did not significantly affect enamel and dentin thickness but still disrupted their development and caused DEJ flattening.

Conclusions

Penicillin at 100 mg/kg adversely affected enamel and dentin development, causing significant defects similar to those caused by tetracycline, although the severity and mechanisms may differ. Even at 50 mg/kg, penicillin disrupted enamel and dentin development, underscoring the clinical relevance of these findings and the need for caution when prescribing penicillin during pregnancy, even at lower doses, due to its potential to disrupt dental development.

目的：青霉素对牙釉质缺陷的影响仍未得到充分研究，特别是在治疗剂量下其引起结构问题的能力方面。材料与方法：妊娠Wistar大鼠从妊娠第13-22天开始，每天灌胃生理盐水（对照组）、四环素130 mg/kg、青霉素50 mg/kg或青霉素100 mg/kg。出生后，幼鼠接受同样的治疗12天。分析上颌第一磨牙的牙釉质/牙本质厚度、成熟度和组织病理学变化。结果：与对照组相比，四环素显著降低了牙釉质和牙本质的厚度，破坏了它们的发育，导致牙本质-牙釉质交界处（DEJ）变平。100 mg/kg的青霉素也显著降低了牙釉质和牙本质的厚度，破坏了它们的成熟，导致DEJ变平。在50 mg/kg剂量下，青霉素对牙本质和牙釉质厚度影响不显著，但仍会破坏牙釉质和牙本质的发育，导致DEJ变平。结论：100mg /kg的青霉素对牙釉质和牙本质发育有不良影响，引起与四环素类似的严重缺陷，尽管严重程度和机制可能不同。即使在50 mg/kg时，青霉素也会破坏牙釉质和牙本质的发育，这强调了这些发现的临床相关性，并且在怀孕期间开青霉素处方时需要谨慎，即使是较低的剂量，因为它有可能破坏牙齿发育。

{"title":"Penicillin Disrupts Dental Mineralization in Rats: A Comparative Study With Tetracycline Highlighting Prenatal and Postnatal Risks","authors":"Sedigheh Mozafar, Fateme Mashhadi Abbas, Majid Mehran, Somayeh Kameli, Motahare Ahmadvand, Amir Mohammad Sharafi, Reza Omid, Morteza Banakar","doi":"10.1002/cre2.70225","DOIUrl":"10.1002/cre2.70225","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Penicillin's impact on enamel defects remains understudied, particularly regarding its ability to cause structural issues even at therapeutic doses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Pregnant Wistar rats received daily gavage from gestational day 13–22 with saline (control), 130 mg/kg tetracycline, 50 mg/kg penicillin, or 100 mg/kg penicillin. After birth, pups received the same treatment for 12 days. Upper first molars were analyzed for enamel/dentin thickness, maturation, and histopathological changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Tetracycline significantly reduced enamel and dentin thickness, disrupted their development, and caused flattening of the dentin-enamel junction (DEJ) compared to the control group. Penicillin at 100 mg/kg also significantly decreased enamel and dentin thickness, impaired their maturation, and led to DEJ flattening. At 50 mg/kg, penicillin did not significantly affect enamel and dentin thickness but still disrupted their development and caused DEJ flattening.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Penicillin at 100 mg/kg adversely affected enamel and dentin development, causing significant defects similar to those caused by tetracycline, although the severity and mechanisms may differ. Even at 50 mg/kg, penicillin disrupted enamel and dentin development, underscoring the clinical relevance of these findings and the need for caution when prescribing penicillin during pregnancy, even at lower doses, due to its potential to disrupt dental development.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Laboratory Contamination of Implant Abutment Screw and Connection on Reverse Torque Value – An In Vitro Study 实验室污染对种植基牙螺钉和连接体反扭矩值的影响-体外研究。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-09-16 DOI: 10.1002/cre2.70222

Hamed Bahrami Maleki, Mona Bazooband, Parviz Amini

Objectives

This in vitro experimental study aimed to evaluate the effects of laboratory contamination of implant abutment screws and connection surfaces on reverse torque values (RTVs), as an indicator of screw preload loss.

Material and Methods

Forty-five Dentis One Q implants and 45 CCM UCLA abutments were randomly assigned into three groups (n = 15 per group). Group 1 (control) involved uncontaminated abutments and screws with no restorations. Group 2 (screw contamination) used new abutments attached with screws contaminated by laboratory materials (porcelain powder, metal debris, and polishing paste). Group 3 (connection contamination) included screw-retained restorations fabricated under contaminated conditions and attached using new screws. All samples were subjected to standardized torque (250 N·cm), thermocycling (1500 cycles between 5°C and 55°C), and subsequent RTV measurement. One-way ANOVA and Tukey's post hoc tests were used for statistical analysis (α = 0.05).

Results

Mean RTVs (SD) were 218 (0.15) N·cm (control), 181 (0.14) N·cm (screw contamination), and 207 (0.11) N·cm (connection contamination). RTVs in the screw contamination group were significantly lower than both the control (p < 0.001) and connection contamination groups (p < 0.001). The difference between the control and connection contamination groups was not statistically significant (p = 0.08).

Conclusions

Laboratory contamination of implant components can significantly reduce reverse torque values, particularly in cases of screw contamination, indicating an increased risk of screw loosening. Contamination control during prosthetic procedures is essential to maintaining implant stability.

目的：本体外实验研究旨在评估实验室污染对反扭矩值（RTVs）的影响，作为螺钉预载荷损失的指标。材料与方法：将45个dental One Q种植体和45个CCM UCLA基牙随机分为3组，每组15个。组1（对照组）为未受污染的基台和螺钉，无修复体。第2组（螺钉污染）使用新基台，用实验室材料（瓷粉、金属碎片、抛光膏）污染的螺钉连接。组3（连接污染）包括在污染条件下制造的螺钉保留修复体，并使用新螺钉连接。所有样品都经过标准化扭矩（250 N·cm）、热循环（在5°C至55°C之间循环1500次）和随后的RTV测量。采用单因素方差分析和Tukey事后检验进行统计学分析（α = 0.05）。结果：平均rtv （SD）分别为218 (0.15)N·cm（对照）、181 (0.14)N·cm（螺钉污染）和207 (0.11)N·cm（连接污染）。螺钉污染组的rtv明显低于对照组(p)。结论：实验室污染的种植体组件可以显著降低反向扭矩值，特别是在螺钉污染的情况下，这表明螺钉松动的风险增加。假体过程中的污染控制对维持假体的稳定性至关重要。

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引用次数: 0