Critical reviews in analytical chemistry最新文献

Biosensors are being studied extensively for their ability to detect and analyze molecules. There has been a growing interest in combining molecular imprinted polymers (MIPs) and aptamers to create hybrid recognition elements that offer advantages such as target binding, sensitivity, selectivity, and stability. These hybrid elements have been successfully used in identifying a wide range of analytes in food samples. However, the application of MIP-based aptasensors in different sensing approaches is still challenging due to the low conductivity of MIPs-aptamers and limited adsorption capacity of MIPs. To address these limitations, researchers have been exploring the use of nanomaterials (NMs) to design efficient multiple-recognition systems that exploit the synergies between aptamers and MIPs. These hybrid systems can enhance the sensitivity and selectivity of MIP-based aptasensors in quantifying analytical samples. This review provides a comprehensive overview of recent advancements in the field of MIP-based aptasensors. It also introduces technologies that combine MIPs and aptamers to achieve higher sensitivity and selectivity in quantifying analytical samples. The review also highlights potential future trends and practical approaches that can be employed to address the limitations of MIP-based aptasensors, including the use of new NMs, the development of new fabrication techniques, and the integration of MIP-based aptasensors with other analytical tools.

Due to their life cycle, viruses can disrupt the metabolism of their hosts, causing diseases. If we want to disrupt their life cycle, it is necessary to identify their presence. For this purpose, it is possible to use several molecular-biological and bioanalytical methods. The reference selection was performed based on electronic databases (2020-2023). This review focused on electrochemical methods with high sensitivity and selectivity (53% voltammetry/amperometry, 33% impedance, and 12% other methods) which showed their great potential for detecting various viruses. Moreover, the aforementioned electrochemical methods have considerable potential to be applicable for care-point use as they are portable due to their miniaturizability and fast speed analysis (minutes to hours), and are relatively easy to interpret. A total of 2011 articles were found, of which 86 original papers were subsequently evaluated (the majority of which are focused on human pathogens, whereas articles dealing with plant pathogens are in the minority). Thirty-two species of viruses were included in the evaluation. It was found that most of the examined research studies (77%) used nanotechnological modifications. Other ones performed immunological (52%) or genetic analyses (43%) for virus detection. 5% of the reports used peptides to increase the method's sensitivity. When evaluable, 65% of the research studies had LOD values in the order of ng or nM. The vast majority (79%) of the studies represent proof of concept and possibilities with low application potential and a high need of further research experimental work.

In precision agriculture, soil spectroscopy has become an invaluable tool for rapid, low-cost, and nondestructive diagnostic approaches. Various instrument configurations are utilized to obtain spectral data over a range of wavelengths, such as homemade sensors, benchtop systems, and mobile instruments. These data are then modeled using a variety of calibration algorithms, including Partial Least Squares Regression (PLSR), Principal Component Regression (PCR), and Support Vector Machines (SVM), these datasets are further improved and optimized. Given the increasing demand for cost-effective and portable solutions, homemade sensors and mobile instruments have gained popularity in recent years. This review paper assesses the current state of soil spectroscopy by comparing the performance, accuracy, precision, and applicability of homemade sensors, mobile spectrometers, and traditional benchtop instruments. The discussion encompasses the technological advancements in homemade sensors, exploring innovative approaches taken by researchers and farmers, as well as developing affordable and efficient soil spectroscopy tools. Mobile and benchtop spectrometers, equipped with cutting-edge technology, have enabled easy soil diagnosis, transforming the landscape of soil analysis.

The amino group is regarded as a multifunctional recognition group in fluorescent probes. It is nucleophilic, a strong electron-donating group and is a polar group with active hydrogen. Based on these characteristics, amino-based fluorescent probes combined with various fluorescent precursors have been constructed, with excellent sensing performance and low cytotoxicity. These probes have significant application value in the detection of food, living cells and organisms. Here, the relevant studies on amino fluorescent probes from 2016 to 2024 are systematically reviewed and their molecular design principles, recognition mechanisms and applications are described. These studies included 14 on exogenous and endogenous formaldehyde detection, five that detected polarity changes in the external environment and organelles in vivo, four intracellular mitochondrial and lysosomal viscosity detections, seven physiological environment and intracellular pH detections, seven metal ion detections in biological and environmental systems and four rapid detections of the hypochlorite anion (ClO^-) in a variety of physiological processes and cells. The application scope of amino fluorescent probes is constantly expanding at present but, research progress in multiple application fields has not been summarized. This article mainly reviews the latest progress in amino fluorescent probes in the fields of food, the environment and the microenvironment, as well as looking forward to the development prospects of these fluorescent probes. Improving the reactivity of amino recognition groups and visual detection may become hot issues in future research.

A living cell is a complex network of molecular, biochemical and physiological processes. Cellular activities, such as ion transport, metabolic processes, and cell-cell interactions can be determined electrochemically by detecting the electrons or ions exchanged in these processes. Electrochemical methods often are noninvasive, and they can enable the real-time monitoring of cellular processes. Scanning electrochemical microscopy (SECM) is an advanced scanning probe electroanalysis technique that can map the surface topography and local reactivity of a substrate with high precision at the micro- or nanoscale. By measuring electrochemical signals, such as redox reactions, ion fluxes, and pH changes, SECM can provide valuable insights into cellular activity. As a result of its compatibility with liquid medium measurements and its nondestructive nature, SECM has gained popularity in living cell research. This review aims to furnish an overview of SECM, elucidating its principles, applications, and its potential to contribute significantly to advancements in cell biology, electroporation, and biosensors. As a multidisciplinary tool, SECM is distinguished by its ability to unravel the intricacies of living cells and offers promising avenues for breakthroughs in our understanding of cellular complexity.