Pub Date : 2023-11-22DOI: 10.2174/0115734137259242231109174821
Qing Wang, Xianyu Li
Background: In our previous studies, we have identified Gsk-3β as a crucial target molecule in response to Danhong injection for cerebral ischemia intervention. Furthermore, it can serve as a molecular imaging probe for medical diagnosis. Bacterial magnetic particles (BMPs), synthesized by magnetotactic bacteria, are regarded as excellent natural nanocarriers. Methods: In this study, we utilized biological modification and chemical crosslinking techniques to produce a multifunctional BMP known as "RVG29-BMP-FA-Gsk-3β-Ab", which exhibits both magnetic properties and brain-targeting capabilities. Then, a combination of analytical techniques was used to characterize the properties of the multifunctional BMPs. Finally, we evaluated the cell targeting ability of the RVG29-BMP-FA-Gsk-3β-Ab. Results: The multifunctional BMPs were observed to possess uniform size and shape using TEM analysis, with a particle size of 70.1±7.33 nm. Zeta potential analysis revealed that the nanoparticles exhibited a regular and non-aggregative distribution of particle sizes. Relative fluorescence intensity results demonstrated that the complex of 1mg of RVG29-BMP-FA-Gsk- 3β-Ab could bind to FITC-RVG29 polypeptide at a concentration of 2189.5 nM. Cell viability analysis indicated its high biocompatibility and minimal cytotoxicity. The RVG29-BMP-FAGsk- 3β-Ab was observed to possess active targeting towards neuronal cells and fluorescence imaging capabilities in vitro, as evidenced by fluorescence imaging assays. The complex of RVG29-BMP-FA-Gsk-3β-Ab exhibited favourable properties for early diagnosis and efficacy evaluation of traditional Chinese medicine in treating cerebral ischemia. Conclusion: This study establishes a fundamental basis for the prospective implementation of multimodal imaging in traditional Chinese medicine for cerebral ischemia.
{"title":"Strategy for Targeting Medical Diagnosis of Cerebral Ischemia Regions by Linking Gsk-3β Antibody and RVG29 to Magnetosomes","authors":"Qing Wang, Xianyu Li","doi":"10.2174/0115734137259242231109174821","DOIUrl":"https://doi.org/10.2174/0115734137259242231109174821","url":null,"abstract":"Background: In our previous studies, we have identified Gsk-3β as a crucial target molecule in response to Danhong injection for cerebral ischemia intervention. Furthermore, it can serve as a molecular imaging probe for medical diagnosis. Bacterial magnetic particles (BMPs), synthesized by magnetotactic bacteria, are regarded as excellent natural nanocarriers. Methods: In this study, we utilized biological modification and chemical crosslinking techniques to produce a multifunctional BMP known as \"RVG29-BMP-FA-Gsk-3β-Ab\", which exhibits both magnetic properties and brain-targeting capabilities. Then, a combination of analytical techniques was used to characterize the properties of the multifunctional BMPs. Finally, we evaluated the cell targeting ability of the RVG29-BMP-FA-Gsk-3β-Ab. Results: The multifunctional BMPs were observed to possess uniform size and shape using TEM analysis, with a particle size of 70.1±7.33 nm. Zeta potential analysis revealed that the nanoparticles exhibited a regular and non-aggregative distribution of particle sizes. Relative fluorescence intensity results demonstrated that the complex of 1mg of RVG29-BMP-FA-Gsk- 3β-Ab could bind to FITC-RVG29 polypeptide at a concentration of 2189.5 nM. Cell viability analysis indicated its high biocompatibility and minimal cytotoxicity. The RVG29-BMP-FAGsk- 3β-Ab was observed to possess active targeting towards neuronal cells and fluorescence imaging capabilities in vitro, as evidenced by fluorescence imaging assays. The complex of RVG29-BMP-FA-Gsk-3β-Ab exhibited favourable properties for early diagnosis and efficacy evaluation of traditional Chinese medicine in treating cerebral ischemia. Conclusion: This study establishes a fundamental basis for the prospective implementation of multimodal imaging in traditional Chinese medicine for cerebral ischemia.","PeriodicalId":10827,"journal":{"name":"Current Nanoscience","volume":"27 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138538992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}