Pub Date : 1900-01-01DOI: 10.1364/omp.2023.oth1e.4
Wenbo Li, Z. Zeng
An integrated platform for multi-algorithm fluorescence fluctuation super-resolution nanoscopy is developed. The platform integrates 4 super-resolution algorithms for image reconstructions. Subcellular details can be discerned at the resolution beyond the diffraction limit using this platform.
{"title":"An Integrated Platform for Multi-Algorithm Fluorescence Fluctuation Super-Resolution Nanoscopy","authors":"Wenbo Li, Z. Zeng","doi":"10.1364/omp.2023.oth1e.4","DOIUrl":"https://doi.org/10.1364/omp.2023.oth1e.4","url":null,"abstract":"An integrated platform for multi-algorithm fluorescence fluctuation super-resolution nanoscopy is developed. The platform integrates 4 super-resolution algorithms for image reconstructions. Subcellular details can be discerned at the resolution beyond the diffraction limit using this platform.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129408137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dth1a.5
A. Joasil, Aidan M. Therien, C. Hendon
The venoatrial junction is an important substrate of atrial fibrillation. We showcase that OCT sub-images of the venoatrial junction can be classified as left atrium or pulmonary vein using a deep CNN with high accuracy.
{"title":"Optical Coherence Tomography Enabled Classification of the Human Venoatrial Junction","authors":"A. Joasil, Aidan M. Therien, C. Hendon","doi":"10.1364/boda.2023.dth1a.5","DOIUrl":"https://doi.org/10.1364/boda.2023.dth1a.5","url":null,"abstract":"The venoatrial junction is an important substrate of atrial fibrillation. We showcase that OCT sub-images of the venoatrial junction can be classified as left atrium or pulmonary vein using a deep CNN with high accuracy.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115864401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dth1a.3
Maryse Lapierre-Landry, Yehe Liu, Mahdi Bayat, D. L. Wilson, Michael W. Jenkins
We propose digital labeling, a method for automated, three-dimensional segmentation of blood vessels without vascular contrast agents. Our deep learning approach greatly simplifies the sample preparation required for 3D microscopy and accelerate image post-processing.
{"title":"Digital Labeling of the Vasculature: Toward Label-Free and Labor-Free Vessel Segmentation","authors":"Maryse Lapierre-Landry, Yehe Liu, Mahdi Bayat, D. L. Wilson, Michael W. Jenkins","doi":"10.1364/boda.2023.dth1a.3","DOIUrl":"https://doi.org/10.1364/boda.2023.dth1a.3","url":null,"abstract":"We propose digital labeling, a method for automated, three-dimensional segmentation of blood vessels without vascular contrast agents. Our deep learning approach greatly simplifies the sample preparation required for 3D microscopy and accelerate image post-processing.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"148 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115913535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.jw2a.3
Mahyar Dahmardeh, Houman Mirzaalian Dastjerdi, Hisham Mazal, Harald Köstler, V. Sandoghdar
Interferometric scattering (iSCAT) microscopy detects single nanoparticles in a label-free fashion. Utilizing self-supervised machine learning pushes the detection sensitivity of iSCAT to very small proteins and disease markers such as chemokines and cytokines.
{"title":"Breaking new ground in protein detection: Self-supervised machine learning and iSCAT enable label-free detection of single proteins below 10 kDa","authors":"Mahyar Dahmardeh, Houman Mirzaalian Dastjerdi, Hisham Mazal, Harald Köstler, V. Sandoghdar","doi":"10.1364/boda.2023.jw2a.3","DOIUrl":"https://doi.org/10.1364/boda.2023.jw2a.3","url":null,"abstract":"Interferometric scattering (iSCAT) microscopy detects single nanoparticles in a label-free fashion. Utilizing self-supervised machine learning pushes the detection sensitivity of iSCAT to very small proteins and disease markers such as chemokines and cytokines.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117265505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.bm4b.5
Anaïs Parrot, Jérémie Guilbert, Pierre Girard-Collins, Michèle Desjardins
Characterizing neurovascular coupling helps to understand neurodegenerative diseases. Here, neuronal activity and vasculature were simultaneously measured in AAV-injected mice through transcranial windows by two-photon microscopy indicating strongly correlated signals synchronized with whiskers stimulations.
{"title":"In Vivo Imaging of Neuronal Activity and Blood Vessels in AAV-Injected Mice","authors":"Anaïs Parrot, Jérémie Guilbert, Pierre Girard-Collins, Michèle Desjardins","doi":"10.1364/brain.2023.bm4b.5","DOIUrl":"https://doi.org/10.1364/brain.2023.bm4b.5","url":null,"abstract":"Characterizing neurovascular coupling helps to understand neurodegenerative diseases. Here, neuronal activity and vasculature were simultaneously measured in AAV-injected mice through transcranial windows by two-photon microscopy indicating strongly correlated signals synchronized with whiskers stimulations.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125408100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.ntu1c.7
Orna Mukhopadhyay
Near-infrared imaging (NIR) combined with machine learning can provide a high-throughput procedure for detecting tumors with high sensitivity and specificity, We present an end-to-end machine learning-based framework for fast, accurate diagnosis of pancreatic carcinoma.
{"title":"Machine Learning-based Analysis of ICG-Assisted NIR Spectral Data for Diagnosing Pancreatic Carcinoma","authors":"Orna Mukhopadhyay","doi":"10.1364/ntm.2023.ntu1c.7","DOIUrl":"https://doi.org/10.1364/ntm.2023.ntu1c.7","url":null,"abstract":"Near-infrared imaging (NIR) combined with machine learning can provide a high-throughput procedure for detecting tumors with high sensitivity and specificity, We present an end-to-end machine learning-based framework for fast, accurate diagnosis of pancreatic carcinoma.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125839328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/brain.2023.btu1b.5
Nischal Khanal, Jonah A. Padawer-Curry, Kevin Schulte, T. Voss, A. Bice, Jin-Moo Lee, A. Bauer
We demonstrate a hybrid method utilizing DeepLabCut and LBMM to concurrently localize motor representations of multiple limbs in mice. Results suggest that motor movements involving multiple limbs reside in overlapping cortical representations of each limb.
{"title":"Deep Learning Driven Light-Based Motor Mapping of Multiple Limb Movements in Mice Reveals Behaviorally Relevant Movements","authors":"Nischal Khanal, Jonah A. Padawer-Curry, Kevin Schulte, T. Voss, A. Bice, Jin-Moo Lee, A. Bauer","doi":"10.1364/brain.2023.btu1b.5","DOIUrl":"https://doi.org/10.1364/brain.2023.btu1b.5","url":null,"abstract":"We demonstrate a hybrid method utilizing DeepLabCut and LBMM to concurrently localize motor representations of multiple limbs in mice. Results suggest that motor movements involving multiple limbs reside in overlapping cortical representations of each limb.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125911894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu3a.1
Guozheng Xu, Isabelle Garnreiter, Thomas J. Smart, Michael G. Chambers, Eduard Durech, Ringo Ng, Jennifer Sun, M. Sarunic
We present our progress on image-guided (wavefront sensor-less) adaptive optics using Deep Learning Methods to optimize the image quality using high numerical aperture OCT and confocal microscopy with a custom developed instrument.
{"title":"Deep Learning Methods for Sensorless Adaptive Optics","authors":"Guozheng Xu, Isabelle Garnreiter, Thomas J. Smart, Michael G. Chambers, Eduard Durech, Ringo Ng, Jennifer Sun, M. Sarunic","doi":"10.1364/boda.2023.dtu3a.1","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu3a.1","url":null,"abstract":"We present our progress on image-guided (wavefront sensor-less) adaptive optics using Deep Learning Methods to optimize the image quality using high numerical aperture OCT and confocal microscopy with a custom developed instrument.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126860228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Paterson, A. Matheson, A. Wright, Tania Mendonca, M. Tassieri, P. Dalgarno
Optical tweezer have been combined with multiplane microscopy to measure particle trajectories in three dimensions and to calculate the viscosity of the suspending media in three dimensions at micrometer length scales. The method is applicable to biological samples.
{"title":"Optical tweezers with integrated multiplane microscopy","authors":"L. Paterson, A. Matheson, A. Wright, Tania Mendonca, M. Tassieri, P. Dalgarno","doi":"10.1364/oma.2023.aw3d.3","DOIUrl":"https://doi.org/10.1364/oma.2023.aw3d.3","url":null,"abstract":"Optical tweezer have been combined with multiplane microscopy to measure particle trajectories in three dimensions and to calculate the viscosity of the suspending media in three dimensions at micrometer length scales. The method is applicable to biological samples.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122532898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/ntm.2023.nth1c.5
C. Liu, W. Eschen, L. Loetgering, D. Molina, R. Klas, A. Iliou, M. Steinert, S. Herkersdorf, A. Kirsche, T. Pertsch, F. Hillmann, J. Limpert, J. Rothhardt
We report on our latest biological imaging results on a tabletop ptychographic microscope at 13.5 nm wavelength. Retrieved amplitude and phase images with sub-60-nm resolution enable the identification of the nanoscale material composition inside microorganisms.
{"title":"Revealing the ultra-structure of microorganisms using tabletop extreme ultraviolet ptychography","authors":"C. Liu, W. Eschen, L. Loetgering, D. Molina, R. Klas, A. Iliou, M. Steinert, S. Herkersdorf, A. Kirsche, T. Pertsch, F. Hillmann, J. Limpert, J. Rothhardt","doi":"10.1364/ntm.2023.nth1c.5","DOIUrl":"https://doi.org/10.1364/ntm.2023.nth1c.5","url":null,"abstract":"We report on our latest biological imaging results on a tabletop ptychographic microscope at 13.5 nm wavelength. Retrieved amplitude and phase images with sub-60-nm resolution enable the identification of the nanoscale material composition inside microorganisms.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129514163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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