Pub Date : 1900-01-01DOI: 10.1364/boda.2023.dtu3a.7
Artem Goncharov, Hyou-Arm Joung, Rajesh Ghosh, Gyeo-Re Han, Zachary S. Ballard, Quinn Maloney, Alexandra Bell, Chew Tin Zar Aung, O. Garner, D. Di Carlo, Aydogan Ozcan
We present a multiplexed paper-based point-of-care sensor processed by a hand-held fluorescence reader and neural networks that quantify three cardiovascular disease biomarkers from human serum samples, achieving a limit-of-detection of ≤0.52 ng/mL for each biomarker.
{"title":"Fluorescence-based multiplexed point-of-care sensor using deep learning","authors":"Artem Goncharov, Hyou-Arm Joung, Rajesh Ghosh, Gyeo-Re Han, Zachary S. Ballard, Quinn Maloney, Alexandra Bell, Chew Tin Zar Aung, O. Garner, D. Di Carlo, Aydogan Ozcan","doi":"10.1364/boda.2023.dtu3a.7","DOIUrl":"https://doi.org/10.1364/boda.2023.dtu3a.7","url":null,"abstract":"We present a multiplexed paper-based point-of-care sensor processed by a hand-held fluorescence reader and neural networks that quantify three cardiovascular disease biomarkers from human serum samples, achieving a limit-of-detection of ≤0.52 ng/mL for each biomarker.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125802190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Cisek, Kennedy Brittain, Macaulay Harvey, Saranyan Pillai, Sean D. Christie, D. Tokarz
Polarization-resolved second harmonic generation microscopy was used to elucidate the arrangement of SHG emitters in a collagen-like protein matrix embedded in mammalian inner ear crystals revealing a radial nano- and macro-arrangement.
{"title":"Measurement of the Crystalline Structure of Collagen-Like Scaffolds of Otoconia in the Mouse Vestibular System by Second Harmonic Generation Microscopy","authors":"R. Cisek, Kennedy Brittain, Macaulay Harvey, Saranyan Pillai, Sean D. Christie, D. Tokarz","doi":"10.1364/omp.2023.om4e.1","DOIUrl":"https://doi.org/10.1364/omp.2023.om4e.1","url":null,"abstract":"Polarization-resolved second harmonic generation microscopy was used to elucidate the arrangement of SHG emitters in a collagen-like protein matrix embedded in mammalian inner ear crystals revealing a radial nano- and macro-arrangement.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125929676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.jtu4b.13
M. Giacomelli
Photomultiplier tubes (PMTs) are the standard for high sensitivity detection of fluorescence signals, but are costly, fragile, have limited QE and worse sensitivity at far-red wavelengths. Newer detectors based on silicon photomultiplier (SiPM) technology can greatly improve sensitivity and imaging rates. The advantages and utilization of silicon photomultiplier technology for high sensitivity and high-speed imaging are reviewed.
{"title":"Improved Sensitivity and Imaging Speeds of Fluorescent Imaging Using Silicon Photomultipliers","authors":"M. Giacomelli","doi":"10.1364/boda.2023.jtu4b.13","DOIUrl":"https://doi.org/10.1364/boda.2023.jtu4b.13","url":null,"abstract":"Photomultiplier tubes (PMTs) are the standard for high sensitivity detection of fluorescence signals, but are costly, fragile, have limited QE and worse sensitivity at far-red wavelengths. Newer detectors based on silicon photomultiplier (SiPM) technology can greatly improve sensitivity and imaging rates. The advantages and utilization of silicon photomultiplier technology for high sensitivity and high-speed imaging are reviewed.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127981080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/omp.2023.otu1e.3
Joy Wang, Pradyumna Kedarisetti, Matthew G. Mallay, Jeremy A. Brown, Frank R. Wuest, R. Zemp
Histotripsy is a powerful precision tool for non-invasive, non-ionizing treatment of tumors, but suffers risk of damage to vasculature under poor visualization. Photoacoustic-guided histotripsy allows for visualization and avoidance of major vasculature and hemorrhage monitoring.
{"title":"Ultrasound and Photoacoustic Image-Guided Micro-Histotripsy for Non-Invasive Surgery","authors":"Joy Wang, Pradyumna Kedarisetti, Matthew G. Mallay, Jeremy A. Brown, Frank R. Wuest, R. Zemp","doi":"10.1364/omp.2023.otu1e.3","DOIUrl":"https://doi.org/10.1364/omp.2023.otu1e.3","url":null,"abstract":"Histotripsy is a powerful precision tool for non-invasive, non-ionizing treatment of tumors, but suffers risk of damage to vasculature under poor visualization. Photoacoustic-guided histotripsy allows for visualization and avoidance of major vasculature and hemorrhage monitoring.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129731458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/oma.2023.ath1d.1
J. Fick
We present an overview of optical trapping of different biological specimen such as human cells, algae or bacteria. Good trapping efficiencies were obtained using distinct micro-structured optical fibers in dual and single fiber geometries.
{"title":"Optical fiber tweezers for biological specimen trapping","authors":"J. Fick","doi":"10.1364/oma.2023.ath1d.1","DOIUrl":"https://doi.org/10.1364/oma.2023.ath1d.1","url":null,"abstract":"We present an overview of optical trapping of different biological specimen such as human cells, algae or bacteria. Good trapping efficiencies were obtained using distinct micro-structured optical fibers in dual and single fiber geometries.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"4 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129226205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1364/boda.2023.jtu4b.19
Aniruddha Das, Julie A. Borovicka, Jacob Icardi, Priyanka Agochiya, Shriya Singh, H. Dana
Concurrent two-photon and third-harmonic generation microscopy recordings from the hippocampus of mice fed with cuprizone to induced demyelination showed a correlation between the severity of myelin loss and reduced neuronal firing in the same mice.
{"title":"Correlated Structural and Functional Deficits in a Mouse Model of Demyelination","authors":"Aniruddha Das, Julie A. Borovicka, Jacob Icardi, Priyanka Agochiya, Shriya Singh, H. Dana","doi":"10.1364/boda.2023.jtu4b.19","DOIUrl":"https://doi.org/10.1364/boda.2023.jtu4b.19","url":null,"abstract":"Concurrent two-photon and third-harmonic generation microscopy recordings from the hippocampus of mice fed with cuprizone to induced demyelination showed a correlation between the severity of myelin loss and reduced neuronal firing in the same mice.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117024346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cell phone microscopes usually suffer from low resolution due to the limitation of size. We propose a PSF-aware deep network in raw domain to perform image restoration task, accomplishing high performance in experimental capture.
{"title":"Cell Phone Microscopy Image Restoration with Deep Prior in Raw Domain","authors":"Xiaofei Song, Liping Ren, Yuanlong Zhang, Jiachen Xie, Jiamin Wu, Haoqian Wang, Qionghai Dai","doi":"10.1364/boda.2023.jtu4a.3","DOIUrl":"https://doi.org/10.1364/boda.2023.jtu4a.3","url":null,"abstract":"Cell phone microscopes usually suffer from low resolution due to the limitation of size. We propose a PSF-aware deep network in raw domain to perform image restoration task, accomplishing high performance in experimental capture.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"83 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121215787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muruga Lokesh, Gokul Nalupurackal, Imdad Uddin Chowdhury, P. S. Mahapatra, Basudev Roy
Optically induced heating of a trapped upconverting particle at the air-water interface of a sessile water droplet creates fluid flows which accelerate the self-assembly of suspended colloidal particles to form a lattice-like structure.
{"title":"Accelerating self-assembly of colloidal particles at air-water interface by laser-induced heating of upconverting particle","authors":"Muruga Lokesh, Gokul Nalupurackal, Imdad Uddin Chowdhury, P. S. Mahapatra, Basudev Roy","doi":"10.1364/oma.2023.aw4d.2","DOIUrl":"https://doi.org/10.1364/oma.2023.aw4d.2","url":null,"abstract":"Optically induced heating of a trapped upconverting particle at the air-water interface of a sessile water droplet creates fluid flows which accelerate the self-assembly of suspended colloidal particles to form a lattice-like structure.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121470711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Villa, E. Tartari, S. Glicenstein, E. Picard, P. Marcoux, M. Zelsmann, G. Resch, E. Hadji, R. Houdré
Rapid analysis of antimicrobial susceptibility is a challenge in the fight against infectious diseases. Here we report how the interaction between integrated optical tweezers and bacteria reveals antibiotic- and bacteriophage-induced physiological stresses.
{"title":"On-Chip Optical Nanotweezers for Ultrafast Antibiotic and Bacteriophage Susceptibility Testing","authors":"N. Villa, E. Tartari, S. Glicenstein, E. Picard, P. Marcoux, M. Zelsmann, G. Resch, E. Hadji, R. Houdré","doi":"10.1364/oma.2023.am2d.2","DOIUrl":"https://doi.org/10.1364/oma.2023.am2d.2","url":null,"abstract":"Rapid analysis of antimicrobial susceptibility is a challenge in the fight against infectious diseases. Here we report how the interaction between integrated optical tweezers and bacteria reveals antibiotic- and bacteriophage-induced physiological stresses.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121393159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Zunino, G. Tortarolo, Francesco Fersini, Giacomo Garré, G. Vicidomini
We introduce a detector array in a laser scanning microscope to perform pixel reassignment and discard the out-of-focus fluorescence light. We enhance the microscope resolution laterally and axially without losing the in-focus photons.
{"title":"Extending the Three-Dimensional Resolution with Focus-ISM","authors":"A. Zunino, G. Tortarolo, Francesco Fersini, Giacomo Garré, G. Vicidomini","doi":"10.1364/ntm.2023.nm2c.4","DOIUrl":"https://doi.org/10.1364/ntm.2023.nm2c.4","url":null,"abstract":"We introduce a detector array in a laser scanning microscope to perform pixel reassignment and discard the out-of-focus fluorescence light. We enhance the microscope resolution laterally and axially without losing the in-focus photons.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"145 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115630544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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