Pub Date : 2011-07-01DOI: 10.5053/EJOBIOS.2011.5.0.7
E. Yalçın, M. Kilinç, H. Kutbay, A. Bilgin, H. Korkmaz
The flora of the grazed and ungrazed alluvial meadows in central the Black Sea Region of Turkey was investigated between 2001 and 2003. The study area is situated along the coast of the central Black Sea Region of Turkey. This area floristically belongs to the Euxine province of the Euro-Siberian phytogeographical region. The studied meadows are used as rangeland. The flora of the study area consists of 29 families, 84 genera, and 122 species and subspecies or varieties. With 19.66% of the plants belonging to, the Euro-Siberian Region, 11.46% are of the Mediterranean floristic region, with 68.85%, of the pluriregional or unknown. The life-form spectrum consists of 69.67% Hemicryptophytes, 22.13% Therophytes, 5.73% Chamaephytes, and 2.45% Geophytes, respectively. The aim of this paper is to define the floristic composition of the natural alluvial meadows in the central Black Sea Region of Turkey. The Flora of Turkey and the East Aegean Islands and The Flora Europea were mainly used to identify the specimens.
{"title":"Floristic properties of lowland meadows in Central Black Sea Region of Turkey","authors":"E. Yalçın, M. Kilinç, H. Kutbay, A. Bilgin, H. Korkmaz","doi":"10.5053/EJOBIOS.2011.5.0.7","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2011.5.0.7","url":null,"abstract":"The flora of the grazed and ungrazed alluvial meadows in central the Black Sea Region of Turkey was investigated between 2001 and 2003. The study area is situated along the coast of the central Black Sea Region of Turkey. This area floristically belongs to the Euxine province of the Euro-Siberian phytogeographical region. The studied meadows are used as rangeland. The flora of the study area consists of 29 families, 84 genera, and 122 species and subspecies or varieties. With 19.66% of the plants belonging to, the Euro-Siberian Region, 11.46% are of the Mediterranean floristic region, with 68.85%, of the pluriregional or unknown. The life-form spectrum consists of 69.67% Hemicryptophytes, 22.13% Therophytes, 5.73% Chamaephytes, and 2.45% Geophytes, respectively. The aim of this paper is to define the floristic composition of the natural alluvial meadows in the central Black Sea Region of Turkey. The Flora of Turkey and the East Aegean Islands and The Flora Europea were mainly used to identify the specimens.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2011.5.0.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70599311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-07-01DOI: 10.5053/EJOBIOS.2011.5.0.14
A. Davoudi, R. Seighalani, S. Aleyasin, A. Tarang, R. Radjabi, Farideh Tahmoressi
Some studies showed analysis of fetal DNA in maternal plasma had been introduced as a new method for non-invasive prenatal diagnosis. Fetal sexing is possible at 8th week of pregnancy, using maternal blood sample testing. The aim was providing a rapid, reliable and non-invasive method for sexing of bovine fetuses. Maternal blood samples were collected from 38 pregnant cows during the 8th-38th w of gestation. Plasma was obtained by centrifugation and DNA was extracted by phenol-chloroform method from 350 L maternal plasma. Two primer pairs for bovine amelogenin, Y-encoded, and testis-specific protein gene were used to amplification three fragments; 260 bp (Y-encoded, testis-specific protein gene), 341 and 467 bp (Y and X chromosome amelogenin gene). The polymerase chain reaction has been optimized for fragments amplification. The 467 bp fragment was detected in all samples. The 341 and 260 bp fragments were detected in 24 of 38 plasma samples of cows with male fetuses. The sensitivity and specificity of test was 100% with no false negative and positive results. The results showed that phenol-chloroform method was a simple and sensational to isolation fetal DNA in maternal plasma. The polymerase chain reaction is a favorable noninvasive method for bovine fetal sexing.
一些研究表明，母体血浆中胎儿DNA的分析已成为一种无创产前诊断的新方法。胎儿性别鉴定是在怀孕第8周，使用母体血液样本检测。目的是提供一种快速、可靠、无创的牛胎儿性别鉴定方法。选取38头妊娠第8 ~ 38周的奶牛进行母血采集。350例L产妇血浆经离心获得血浆，用苯酚-氯仿法提取DNA。分别用牛淀粉原蛋白、y编码基因和睾丸特异性蛋白基因的两对引物扩增出三个片段;260 bp (Y编码，睾丸特异性蛋白基因)，341和467 bp (Y和X染色体淀粉原基因)。聚合酶链反应已优化片段扩增。在所有样本中均检测到467 bp的片段。38例有男胎奶牛血浆样品中有24例检测到341和260 bp的片段。检测的敏感性和特异性均为100%，无假阴性和阳性结果。结果表明，苯酚-氯仿法是一种简便、有效的分离母体血浆中胎儿DNA的方法。聚合酶链反应是一种良好的无创牛胎儿性别鉴定方法。
{"title":"The application of amplified TSPY and amelogenin genes from maternal plasma as a non-invasive bovine fetal DNA diagnosis","authors":"A. Davoudi, R. Seighalani, S. Aleyasin, A. Tarang, R. Radjabi, Farideh Tahmoressi","doi":"10.5053/EJOBIOS.2011.5.0.14","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2011.5.0.14","url":null,"abstract":"Some studies showed analysis of fetal DNA in maternal plasma had been introduced as a new method for non-invasive prenatal diagnosis. Fetal sexing is possible at 8th week of pregnancy, using maternal blood sample testing. The aim was providing a rapid, reliable and non-invasive \u0000method for sexing of bovine fetuses. Maternal blood samples were collected from 38 pregnant cows during the 8th-38th w of gestation. Plasma was obtained by centrifugation and DNA was extracted by phenol-chloroform method from 350 L maternal plasma. Two primer pairs for bovine amelogenin, Y-encoded, and testis-specific protein gene were used to amplification three fragments; 260 bp (Y-encoded, testis-specific protein gene), 341 and 467 bp (Y and X chromosome amelogenin gene). The polymerase chain reaction has been optimized for fragments amplification. The 467 bp fragment was detected in all samples. The 341 and 260 bp fragments were detected in 24 of 38 plasma samples of cows with male fetuses. The sensitivity and specificity of test was 100% with no false negative and positive results. The results showed that phenol-chloroform method was a simple and sensational to isolation fetal DNA in maternal plasma. The polymerase chain reaction is a favorable noninvasive method for bovine fetal sexing.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2011.5.0.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70598835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-07-01DOI: 10.5053/EJOBIOS.2011.5.0.13
M. Mollah, K. Taslima, H. Rashid, Z. Hossain, M. Sarowar, Muhammad Rakibul Kabir Khan
The present study was carried out to investigate the embryonic and larval development of freshwater catfish Rita rita. The mature eggs and sperms were collected by using artificial insemination technique and fertilized eggs were incubated in mini circular hatchery with provision of continuous water supply. The fertilized eggs were transparent, demarsal, spherical, non-adhesive and brownish in colour with a diameter ranging between 1.3 to 1.6 mm. First cleavage occurred within 25-30 min post-fertilization at temperature of 28±1°C. Hatching started 22 h post-fertilization and completed within 24 h at the same temperature range. Newly hatched larvae were 2.0 mm in length devoid of mouth and pigmentation and started feeding within 48-60 h post-hatching. To date, this is the first time the early embryonic and larval development of freshwater catfish R. rita is described. Thus the findings of the present study provide valuable information that may help establishing the large scale seed production technique of Rita.
{"title":"Embryonic and larval development of critically endangered riverine catfish Rita rita","authors":"M. Mollah, K. Taslima, H. Rashid, Z. Hossain, M. Sarowar, Muhammad Rakibul Kabir Khan","doi":"10.5053/EJOBIOS.2011.5.0.13","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2011.5.0.13","url":null,"abstract":"The present study was carried out to investigate the embryonic and larval development of freshwater catfish Rita rita. The mature eggs and sperms were collected by using artificial insemination technique and fertilized eggs were incubated in mini circular hatchery with provision of continuous water supply. The fertilized eggs were transparent, demarsal, spherical, non-adhesive and brownish in colour with a diameter ranging between 1.3 to 1.6 mm. First cleavage occurred within 25-30 min post-fertilization at temperature of 28±1°C. Hatching started 22 h post-fertilization and completed within 24 h at the same temperature range. Newly hatched larvae were 2.0 mm in length devoid of mouth and pigmentation and started feeding within 48-60 h post-hatching. To date, this is the first time the early embryonic and larval development of freshwater catfish R. rita is described. Thus the findings of the present study provide valuable information that may help establishing the large scale seed production technique of Rita.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2011.5.0.13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70598728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-01-01DOI: 10.5053/EJOBIOS.2011.5.0.1
F. Uyar, Ilknur Porsuk, G. Kızıl, E. Yilmaz
An alkaline protease producer Bacillus sp. strain CA15 was isolated from soil. The microorganism was found to be closely related to Bacillus cereus based on 16S ribosomal DNA sequencing. The culture conditions for higher protease production were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% skim milk, 1% starch and 0.6% MgSO 4 .7H 2 O, initial pH 8.0 at 35°C. The best enzyme production was obtained during the stationary phase in which the cell density reached to 1.8x108 cells/mL. The level of protease was found to be low in the presence of inorganic nitrogen sources. The protease production was diminished in the presence of sucrose and lactose. The extreme stability towards Triton X-100, Tween 20 and SDS was observed by Bacillus sp. CA15 alkaline protease. The enzyme activity was inhibited by PMSF suggested that presence of serine residues at the active sites.
{"title":"Optimal conditions for production of extracellular protease from newly isolated Bacillus cereus strain CA15","authors":"F. Uyar, Ilknur Porsuk, G. Kızıl, E. Yilmaz","doi":"10.5053/EJOBIOS.2011.5.0.1","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2011.5.0.1","url":null,"abstract":"An alkaline protease producer Bacillus sp. strain CA15 was isolated from soil. The microorganism was found to be closely related to Bacillus cereus based on 16S ribosomal DNA sequencing. The culture conditions for higher protease production were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% skim milk, 1% starch and 0.6% MgSO 4 .7H 2 O, initial pH 8.0 at 35°C. The best enzyme production was obtained during the stationary phase in which the cell density reached to 1.8x108 cells/mL. The level of protease was found to be low in the presence of inorganic nitrogen sources. The protease production was diminished in the presence of sucrose and lactose. The extreme stability towards Triton X-100, Tween 20 and SDS was observed by Bacillus sp. CA15 alkaline protease. The enzyme activity was inhibited by PMSF suggested that presence of serine residues at the active sites.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2011.5.0.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70598516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-01DOI: 10.5053/EJOBIOS.2010.4.0.12
S. I. M. Houimli, M. Denden, Bouthaina Dridi Mouhandes
Brassinosteroids are steroidal phytohormones that have the ability to overcome plant environmental stress. This study was carried out to investigate the role of 24-epibrassinolide in inducing pepper plant salt tolerance as measured by a range of physiological parameters: growth, chlorophyll, electrolyte leakage and proline. Capsicum annuum cv. Beldi seedlings were sprayed with 24-epibrassinolide both in the presence or the absence of NaCl and were sampled, 28 days after treatments. As a result of analysing the cultures under salinity stress, it was determined that the biomass and the chlorophyll decreased significantly, while the electrolyte leakage and the proline concentration increased considerably under salinity stress. However, the application of 24-epibrassinolide significantly ameliorated the adverse effects of salinity on the examined parameters, confirming the suppositions of previous authors who have claimed that exogenously applied 24-epibrassinolide can increase growth and protect the integrity of the cellular membrane in stressed plants.
{"title":"Effects of 24-epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress","authors":"S. I. M. Houimli, M. Denden, Bouthaina Dridi Mouhandes","doi":"10.5053/EJOBIOS.2010.4.0.12","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.12","url":null,"abstract":"Brassinosteroids are steroidal phytohormones that have the ability to overcome plant environmental stress. This study was carried out to investigate the role of 24-epibrassinolide in inducing pepper plant salt tolerance as measured by a range of physiological parameters: growth, chlorophyll, electrolyte leakage and proline. Capsicum annuum cv. Beldi seedlings were sprayed with 24-epibrassinolide both in the presence or the absence of NaCl and were sampled, 28 days after treatments. As a result of analysing the cultures under salinity stress, it was determined that the biomass and the chlorophyll decreased significantly, while the electrolyte leakage and the proline concentration increased considerably under salinity stress. However, the application of 24-epibrassinolide significantly ameliorated the adverse effects of salinity on the examined parameters, confirming the suppositions of previous authors who have claimed that exogenously applied 24-epibrassinolide can increase growth and protect the integrity of the cellular membrane in stressed plants.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-01DOI: 10.5053/EJOBIOS.2010.4.0.15
N. Mittal, A. Dubey
A novel set of polymorphic ISSR and chloroplast microsatellite markers were identified to mark the genetic relationship among different accessions of Jatropha curcas. A total of 12 polymorphic ISSR primers used, amplified a total of 26 fragments among ten different accessions of J. curcas. The cross-species amplification of microsatellite loci is timesaving as well as a cost-effective approach for developing locus specific markers for new species. Further, in an attempt to reveal a high degree of polymorphism and genetic variation among different accessions of J. curcas chloroplast microsatellite primer pairs developed for Acorus calamus (Acoraceace) were examined for cross-species amplification and validation among them. Out of 18 cpSSRs, six (33.33%) were identified as resulting in good amplifications and polymorphism. The number of amplified alleles per cpSSR primer pair ranged from 2 to 4 with the total number of 18 size variants as whole. The 18 size variants at 6 loci among 10 accessions were combined in 4 different haplotypes. The total chloroplast DNA diversity (HT) and the differentiation (GST) were found as 0.700 and 0.560 respectively. Whereas, Shannon's information index (I), Nei's genetic diversity among different accessions of J. curcas using ISSR and cpSSR markers was estimated at 0.33±0.28; 0.22±0.19 and 0.94±0.35; 0.55±0.18 respectively. The genetic relationship among the accessions was established using UPGMA cluster analysis, which were grouped into two major clusters denoted as cluster-I and cluster-II. The implication of the results in devising the importance of a identified novel set of markers for the genetic relationship and genetic diversity analysis in J. curcas is discussed.
{"title":"A novel set of highly polymorphic chloroplast microsatellite and ISSR markers for the biofuel crop Jatropha curcas","authors":"N. Mittal, A. Dubey","doi":"10.5053/EJOBIOS.2010.4.0.15","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.15","url":null,"abstract":"A novel set of polymorphic ISSR and chloroplast microsatellite markers were identified to mark the genetic relationship among different accessions of Jatropha curcas. A total of 12 polymorphic ISSR primers used, amplified a total of 26 fragments among ten different accessions of J. curcas. The cross-species amplification of microsatellite loci is timesaving as well as a cost-effective approach for developing locus specific markers for new species. Further, in an attempt to reveal a high degree of polymorphism and genetic variation among different accessions of J. curcas chloroplast microsatellite primer pairs developed for Acorus calamus (Acoraceace) were examined for cross-species amplification and validation among them. Out of 18 cpSSRs, six (33.33%) were identified as resulting in good amplifications and polymorphism. The number of amplified alleles per cpSSR primer pair ranged from 2 to 4 with the total number of 18 size variants as whole. The 18 size variants at 6 loci among 10 accessions were combined in 4 different haplotypes. The total chloroplast DNA diversity (HT) and the differentiation (GST) were found as 0.700 and 0.560 respectively. Whereas, Shannon's information index (I), Nei's genetic diversity among different accessions of J. curcas using ISSR and cpSSR markers was estimated at 0.33±0.28; 0.22±0.19 and 0.94±0.35; 0.55±0.18 respectively. The genetic relationship among the accessions was established using UPGMA cluster analysis, which were grouped into two major clusters denoted as cluster-I and cluster-II. The implication of the results in devising the importance of a identified novel set of markers for the genetic relationship and genetic diversity analysis in J. curcas is discussed.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.15","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-01DOI: 10.5053/EJOBIOS.2010.4.0.14
D. Egamberdieva
Many studies provide evidence for the occurrence of opportunistic pathogens in the rhizosphere of plants. The presence of a competitive microflora in soil may restrict root colonization by potentially pathogenic bacteria. The aim of the present work was to study the competitive tomato root tip colonization of potentially human pathogenic bacteria such as Bacillus cereus, Staphylacoccus saprophyticus, Pseudomonas aeruginosa, Acinetobacter sp. against best known root tip colonizer P. fluorescens WCS365 and to characterize for their ability to stimulate plant growth and to suppress tomato foot and root rot disease caused by Fusarium oxysporum f.sp. radicis-lycopersici. In competitive tomato root tip colonization assays all tested bacterial strains showed poor colonization of the tomato rhizosphere except P. aeruginosa which was better competitive colonizer than reference strains. All five bacterial strains stimulated shoot length (up to 35%) and dry matter (up to 58%) of tomato and only one strain B. cereus TSAU80 showed statistically significant disease control (27%) of tomato foot and root rot in comparison to the Fusarium-infected control plants (47%). These results show that the opportunistic pathogens possess beneficial effect on plant growth and are able to colonize the rhizosphere of tomato, potentially resulting risk of contamination for tomato greenhouses. However, their competitive colonization abilities in the rhizosphere are poor, whereas only P. aeruginosa appeared to be better competitive colonizer than reference strain.
{"title":"Colonization of tomato roots by some potentially human-pathogenic bacteria and their plant-beneficial properties","authors":"D. Egamberdieva","doi":"10.5053/EJOBIOS.2010.4.0.14","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.14","url":null,"abstract":"Many studies provide evidence for the occurrence of opportunistic pathogens in the rhizosphere of plants. The presence of a competitive microflora in soil may restrict root colonization by potentially pathogenic bacteria. The aim of the present work was to study the competitive tomato root tip colonization of potentially human pathogenic bacteria such as Bacillus cereus, Staphylacoccus saprophyticus, Pseudomonas aeruginosa, Acinetobacter sp. against best known root tip colonizer P. fluorescens WCS365 and to characterize for their ability to stimulate plant growth and to suppress tomato foot and root rot disease caused by Fusarium oxysporum f.sp. radicis-lycopersici. In competitive tomato root tip colonization assays all tested bacterial strains showed poor colonization of the tomato rhizosphere except P. aeruginosa which was better competitive colonizer than reference strains. All five bacterial strains stimulated shoot length (up to 35%) and dry matter (up to 58%) of tomato and only one strain B. cereus TSAU80 showed statistically significant disease control (27%) of tomato foot and root rot in comparison to the Fusarium-infected control plants (47%). These results show that the opportunistic pathogens possess beneficial effect on plant growth and are able to colonize the rhizosphere of tomato, potentially resulting risk of contamination for tomato greenhouses. However, their competitive colonization abilities in the rhizosphere are poor, whereas only P. aeruginosa appeared to be better competitive colonizer than reference strain.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-01DOI: 10.5053/EJOBIOS.2010.4.0.11
M. Ahemad, M. S. Khan
Four fungicides, tebuconazole, hexaconazole, metalaxyl and kitazin were applied at recommended and higher rates to examine their effects on the plant growth promoting activities of the fungicide tolerant Enterobacter asburiae strain PS2 under in vitro conditions. Enterobacter asburiae strain PS2 was isolated from the mustard rhizosphere and was assessed for the fungicide-tolerance and production of plant growth promoting traits (phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production) both in the presence and absence of fungicides. Enterobacter asburiae strain PS2 showed plant growth promoting activities even in the presence of fungicides which however, decreased progressively with the increase in fungicide concentration. Fungicides at recommended dose had little effect while the dose higher than the recommended one adversely affected the physiological traits, like, phosphate solubilization, siderophore, and indole acetic acid synthesis. Among all fungicides, tebuconazole in general, showed maximum toxicity to plant growth promoting activities. Fungicide-tolerant E. asburiae strain PS2 with the inherent ability to produce growth regulators even in the presence of fungicides can be exploited as a bio-inoculant to increase the productivity of crops grown in fungicide stressed soils.
{"title":"Plant growth promoting activities of phosphate- solubilizing Enterobacter asburiae as influenced by fungicides","authors":"M. Ahemad, M. S. Khan","doi":"10.5053/EJOBIOS.2010.4.0.11","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.11","url":null,"abstract":"Four fungicides, tebuconazole, hexaconazole, metalaxyl and kitazin were applied at recommended and higher rates to examine their effects on the plant growth promoting activities of the fungicide tolerant Enterobacter asburiae strain PS2 under in vitro conditions. Enterobacter asburiae strain PS2 was isolated from the mustard rhizosphere and was assessed for the fungicide-tolerance and production of plant growth promoting traits (phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production) both in the presence and absence of fungicides. Enterobacter asburiae strain PS2 showed plant growth promoting activities even in the presence of fungicides which however, decreased progressively with the increase in fungicide concentration. Fungicides at recommended dose had little effect while the dose higher than the recommended one adversely affected the physiological traits, like, phosphate solubilization, siderophore, and indole acetic acid synthesis. Among all fungicides, tebuconazole in general, showed maximum toxicity to plant growth promoting activities. Fungicide-tolerant E. asburiae strain PS2 with the inherent ability to produce growth regulators even in the presence of fungicides can be exploited as a bio-inoculant to increase the productivity of crops grown in fungicide stressed soils.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-01DOI: 10.5053/EJOBIOS.2010.4.0.8
Patchara Pedpradab, Wanlapa Molex, V. Nukoolkarn, Udomsak Darumas
Thirty six organic extracts were prepared from eighteen marine sponges collected from the Andaman Sea, Thailand. The extracts were examined for anti-malaria, anti-Microbacterium tuberculosis, anti-herpes simplex virus, antimicrobial, anti-acetylcholinesterase enzyme and cytotoxic activities. Four extracts showed anti-M. tuberculosis, one anti-malarial, twenty four antimicrobial and one extract exhibited cytotoxic activity. However, anti-acetylcholinesterase enzyme and anti-herpes simplex virus type 1 (HSV-1) activities were not recorded. Dichloromethane extracts prepared from Axinyssa sp., Halichondria sp. and Chondrosia reticulata exhibited potential anti-M. tuberculosis at MIC 50, 100 and 200 mg/mL, respectively. The Hexane part of Phakellia ventilabrum extract showed anti-malarial activity (MIC= 2.8 mg/mL) while the dichloromethane extract showed anti-M. tuberculosis and cytotoxic activity with MIC 200 and IC50 7.1 mg/mL, respectively. Antimicrobial activity was found in both the hexane and dichloromethane parts of extracts.
{"title":"Biological activities of extracts from Andaman Sea sponges, Thailand","authors":"Patchara Pedpradab, Wanlapa Molex, V. Nukoolkarn, Udomsak Darumas","doi":"10.5053/EJOBIOS.2010.4.0.8","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.8","url":null,"abstract":"Thirty six organic extracts were prepared from eighteen marine sponges collected from the Andaman Sea, Thailand. The extracts were examined for anti-malaria, anti-Microbacterium tuberculosis, anti-herpes simplex virus, antimicrobial, anti-acetylcholinesterase enzyme and cytotoxic activities. Four extracts showed anti-M. tuberculosis, one anti-malarial, twenty four antimicrobial and one extract exhibited cytotoxic activity. However, anti-acetylcholinesterase enzyme and anti-herpes simplex virus type 1 (HSV-1) activities were not recorded. Dichloromethane extracts prepared from Axinyssa sp., Halichondria sp. and Chondrosia reticulata exhibited potential anti-M. tuberculosis at MIC 50, 100 and 200 mg/mL, respectively. The Hexane part of Phakellia ventilabrum extract showed anti-malarial activity (MIC= 2.8 mg/mL) while the dichloromethane extract showed anti-M. tuberculosis and cytotoxic activity with MIC 200 and IC50 7.1 mg/mL, respectively. Antimicrobial activity was found in both the hexane and dichloromethane parts of extracts.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70597901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-07-01DOI: 10.5053/EJOBIOS.2010.4.0.7
N. Kandemir
The morphological and anatomical characteristics of Crocus reticulatus subsp. hittiticus and Crocus cancellatus subsp. lycius, which are under the danger of extinction, have been investigated. The distributions of the two endemic subspecies are limited in Turkey. The plant samples were collected from the South Anatolia Region. The subsp. hittiticus flowers in the early spring whereas subsp. lycius flowers in the autumn. Their corm tunics are coarsely reticulate-fibrous. The subsp. hittiticus has blackish anthers and purple striped outer segments. The subsp. lycius has flowers with very pale lilac coloured and veined. Also, the style of this subsp. is equal to their anthers. These morphological properties are characteristics for two the subspecies investigated. The transverse-sections of the root, stem and leaf parts of these subspecies have been examined and revealed. Their morphologic and anatomic structures are illustrated. The anatomic properties of the two subspecies have been determined to be similar to the anatomic properties of other species of the genus Crocus. Moreover, the crystal type on the organs of the subsp. lycius was investigated.
{"title":"A morphological and anatomical investigation about two rare and endemic Crocus taxa (Iridaceae) from Southern Anatolia","authors":"N. Kandemir","doi":"10.5053/EJOBIOS.2010.4.0.7","DOIUrl":"https://doi.org/10.5053/EJOBIOS.2010.4.0.7","url":null,"abstract":"The morphological and anatomical characteristics of Crocus reticulatus subsp. hittiticus and Crocus cancellatus subsp. lycius, which are under the danger of extinction, have been investigated. The distributions of the two endemic subspecies are limited in Turkey. The plant samples were collected from the South Anatolia Region. The subsp. hittiticus flowers in the early spring whereas subsp. lycius flowers in the autumn. Their corm tunics are coarsely reticulate-fibrous. The subsp. hittiticus has blackish anthers and purple striped outer segments. The subsp. lycius has flowers with very pale lilac coloured and veined. Also, the style of this subsp. is equal to their anthers. These morphological properties are characteristics for two the subspecies investigated. The transverse-sections of the root, stem and leaf parts of these subspecies have been examined and revealed. Their morphologic and anatomic structures are illustrated. The anatomic properties of the two subspecies have been determined to be similar to the anatomic properties of other species of the genus Crocus. Moreover, the crystal type on the organs of the subsp. lycius was investigated.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2010.4.0.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70598161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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