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Encapsulation of black cumin seed (Nigella sativa) oil by using inverse gelation method 反凝胶法包封黑孜然籽油
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100089
Recep Palamutoğlu, Cemal Kasnak, Buket Özen

Black cumin seed oil (BCO) is an important oil source in the food industry. Alginate capsules with aqueous cores can be made by reversing the gelation technique with alginate and CaCl2. The research aims to investigate the physicochemical properties of the capsules as well as to analyze the storage stability of BCO emulsions (emulgators; polyglycerol polyricinoleate (A) and sorbitan monooleate (B)) coated using the inverse gelation method. The peroxide value in group A increased rapidly on the first day and then declined without any significant difference between the third and sixth days (p > 0.05). The peroxide value of group B increased significantly (p < 0.05) on the first day and then declined like in the other groups. BCO (7.42 ± 0.16) had the lowest p-anisidine value at the beginning with the initial p-anisidine value of encapsulated BCO groups A and B (21.74 ± 0.84 and 11.48 ± 1.31, respectively) having significantly higher (p < 0.05). The reverse gelation technique utilized in this study raised the p-anisidine value while lowering the peroxide value of the seed oil. The shelf life of black seed oil can be increased by using this technique in the industry.

黑孜然籽油(BCO)是食品工业中重要的油源。用海藻酸盐和CaCl2逆转凝胶技术可制得带水芯的海藻酸盐胶囊。本研究旨在研究BCO胶囊的物理化学性质,并分析BCO乳剂(乳化剂)的储存稳定性;聚甘油聚蓖麻油酸酯(A)和山梨糖单油酸酯(B))用反凝胶法包被。A组的过氧化值在第1天迅速上升,然后下降,第3天和第6天无显著差异(p >0.05)。B组过氧化值显著升高(p <0.05),随后与其他组一样下降。BCO初始p-茴香胺值最低(7.42±0.16),A组和B组的p-茴香胺初始值分别为21.74±0.84和11.48±1.31,显著高于对照组(p <0.05)。本研究采用的反凝胶技术提高了对茴香胺值,同时降低了种子油的过氧化值。在工业上应用该技术可延长黑籽油的保质期。
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引用次数: 1
Development and characterization of griseofulvin loaded nanostructured lipid carrier gel for treating dermatophytosis 灰黄霉素纳米结构脂质载体凝胶治疗皮肤病的研制与表征
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100074
Neelam Datt , Rajasekhar Reddy Poonuru , Pankaj K. Yadav

The present investigations were aimed at formulating topical gel containing nanostructured lipid carriers (NLCs) of griseofulvin and assess its effectiveness on superficial infections. The drug solubility studies were executed using various lipids and surfactants like Glyceryl monostearate, Oleic acid, Pluronic F 68, and Tween 80, and the concentrations of lipids, surfactants, and emulsifier were optimized using Box-Behnken design (BBD). Microemulsions were made utilizing sonication. The prepared batches (F1 to F15) were analyzed and observed that the optimized batch (F12), containing 0.2% w/w drug, 2% GMS, 2% Pluronic F68 and Tween 80 (in the ratio of 1:1) showed a particle size of 209 nm, zeta potential of -44.12 mV, entrapment level of 85.24% along with a drug release of 92.12%. Carbopol 940, 1.5% was used to make the topical gel. The results of biochemical studies reflected that griseofulvin-loaded-nanogel produced a more significant decrease in lipid peroxidation as compared to the standard drug. The in-vitro cytotoxicity studies showed better safety of nanogel in human keratinocyte cells (HaCaT). The results of antifungal activity showed complete clinical and mycological cure in a duration of 21 days against superficial infections like Tenia pedis and also ringworm in Wistar rats while using T.rubrum and M.canis fungal strains. These preclinical investigations have proved that the nanogels have a better potential in treating the aforementioned superficial infections providing an effective alternative for currently existing products.

本研究旨在制备含有灰黄霉素纳米结构脂质载体(nlc)的局部凝胶,并评估其对浅表感染的有效性。采用单硬脂酸甘油、油酸、Pluronic f68和Tween 80等多种脂类和表面活性剂进行药物溶解度研究,并采用Box-Behnken设计(BBD)对脂类、表面活性剂和乳化剂的浓度进行优化。利用超声法制备微乳液。对制备的批(F1 ~ F15)进行分析,发现最佳批(F12)的粒径为209 nm, zeta电位为-44.12 mV,包封率为85.24%,释药率为92.12%。F12为0.2% w/w药物、2% GMS、2% Pluronic F68和Tween 80(比例为1:1)。卡波波尔940,1.5%制备外用凝胶。生化研究结果表明,与标准药物相比,负载灰黄蛋白的纳米凝胶产生了更显著的脂质过氧化降低。体外细胞毒性研究表明纳米凝胶在人角质形成细胞(HaCaT)中的安全性较好。抗真菌活性的结果表明,在21天的时间内,使用红毛霉和狗毛霉菌株对Wistar大鼠的足癣和癣等浅表感染有完全的临床和真菌学治愈。这些临床前研究证明纳米凝胶在治疗上述表面感染方面具有更好的潜力,为现有产品提供了有效的替代方案。
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引用次数: 3
Synthesis of N, N, N-trimethyl chitosan-based nanospheres for the prolonged release of curcumin N, N, N-三甲基壳聚糖纳米微球的合成及其对姜黄素缓释的影响
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100092
Xueqin Zhao , Chao Lu , Songlin Yang , Rui Ni , Tianqing Peng , Jin Zhang

It is critical to develop a hydrophilic drug carrier with positive charge on the surface to enhance the bioavailability of curcumin to overcome the tissue barrier, e.g., blood brain barrier. In this paper, a quaternized chitosan derivative, N,N,N-Trimethyl Chitosan (TMC) was produced which is a cationic polysaccharide. Nuclear magnetic resonance (1H-NMR) and Infrared Spectroscopy (FTIR) have been used to verify the synthesis of TMC. A simple nanoemulsion process has been developed to produce TMC-based nanosphere to load curcumin. A high encapsulation efficiency (over 90%) can be observed. The average particle size of nanospheres made of TMC with 2.5 mg/mL and 4.0 mg/ mL is estimated at 555.3±117.7 nm and 771.2±123.2 nm, respectively. The effect of the concentrations of TMC on the release profile has been investigated. It is found that nanospheres made of a higher concentration of TMC, 4.0 mg/mL, could lead to an extended release of curcumin, and the first-order release kinetics can be observed when release time increases from 0 to 265 h. The release kinetics of curcumin loaded in TMC nanospheres is also influenced by pH value. In addition, the cytotoxicity study shows that no toxic effect can be found when cells are treated with synthetic TMC. The relative cell viability of mouse cardiac endothelial cells treated with curcumin loaded TMC nanospheres is higher than that when cells treated with curcumin alone.

为了提高姜黄素的生物利用度,克服组织屏障,如血脑屏障,开发一种表面带正电荷的亲水性药物载体至关重要。本文制备了一种季铵化壳聚糖衍生物N,N,N-三甲基壳聚糖(TMC),它是一种阳离子多糖。利用核磁共振(1H-NMR)和红外光谱(FTIR)对TMC的合成进行了验证。研究了一种简单的纳米乳法制备负载姜黄素的纳米球。可观察到高封装效率(90%以上)。2.5 mg/mL和4.0 mg/mL的TMC制备的纳米球的平均粒径分别为555.3±117.7 nm和771.2±123.2 nm。研究了TMC浓度对其释放曲线的影响。结果表明,当TMC的浓度达到4.0 mg/mL时,姜黄素的释放时间延长,且随着释放时间的增加,姜黄素的释放动力学呈一级释放,且其释放动力学也受pH值的影响。此外,细胞毒性研究表明,用合成TMC处理细胞时,没有发现毒性作用。姜黄素负载TMC纳米球处理的小鼠心脏内皮细胞的相对细胞活力高于单独姜黄素处理的细胞。
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引用次数: 2
Recent progress in alginate-based carriers for ocular targeting of therapeutics 以海藻酸盐为基础的眼部靶向治疗载体的研究进展
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100071
Sandip Karmakar , Sreejan Manna , Sourav Kabiraj , Sougata Jana

Eye is one of the susceptible organs of the human body which is associated with several internal organs including the brain. Due to the protective mechanisms of body and ocular barrier properties, the area of ocular drug delivery presents a challenge to pharmaceutical researchers. Alginate is a naturally occurring polysaccharide obtained from marine brown seaweeds and bacterial sources. According to the Food and Drug Administration, (FDA) alginate is generally recognized as safe (GRAS). It is widely used as a gelling agent and thickener in the food industries. In the field of drug delivery, alginate is extensively investigated as an excipient. In ocular targeting of therapeutics, sodium alginate offers many advantages including ion sensitive in situ gelation, non-toxic and biodegradable behaviour in combination with mucoadhesive nature of the polymer. The instant gelation ability of alginate allows it to increase the ocular residence time and enhances the ocular drug bioavailability reducing the requirement of frequent administration of drug. The abundant availability and attractive physicochemical properties of alginate has encouraged pharmaceutical scientists to explore newer strategies in ocular drug targeting. In this review, the efficacy of alginate in delivering various therapeutic agents has been discussed.

眼睛是人体的易感器官之一,它与包括大脑在内的几个内脏器官都有联系。由于机体和眼屏障特性的保护机制,眼内给药成为药学研究的一个挑战。藻酸盐是一种天然存在的多糖,从海洋棕色海藻和细菌来源中获得。根据美国食品和药物管理局(FDA),海藻酸盐通常被认为是安全的(GRAS)。在食品工业中广泛用作胶凝剂和增稠剂。在给药领域,海藻酸盐作为一种赋形剂被广泛研究。在眼部靶向治疗中,海藻酸钠具有许多优点,包括离子敏感的原位凝胶,无毒和可生物降解的行为,以及聚合物的黏附性。海藻酸盐的即时凝胶能力使其能够增加眼部停留时间,提高眼部药物的生物利用度,减少频繁给药的需要。海藻酸盐丰富的可用性和吸引人的理化性质促使药理学家探索眼部药物靶向治疗的新策略。本文综述了海藻酸盐在各种治疗药物输送中的作用。
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引用次数: 17
Using pure Fucoidan and radiolabeled Fucoidan (99mTc-Fucoidan) as a new agent for inflammation diagnosis and therapy 使用纯岩藻糖聚糖和放射性标记岩藻糖聚糖(99mtc -岩藻糖聚糖)作为炎症诊断和治疗的新药物
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2021.100049
Bianca Costa , Luana Barbosa Corrêa , Patrícia MachadoRodrigues e Silva , Yago Amigo Pinho Jannini de Sá , Fernanda Verdini Guimarães , Luciana Magalhães Rebelo Alencar , Rafael Loureiro Simões , Edward Helal-Neto , Eduardo Ricci-Junior , Maria das Graças Muller de Oliveira Henriques , Elaine Cruz Rosas , Ralph Santos-Oliveira

Inflammation is a phenomenon responsible for the perturbation of homeostasis in several levels, with many sources, such as infection, injury, and exposure to contaminants. The necessity of new products that are effective in treating inflammation processes as can selectively imaging an inflammation site is a global issue. In this study, we have evaluated the applicability of Fucoidan as a therapeutic and imaging agent. We have assessed the Fucoidan in two inflammation models for therapeutic purposes: arthritis and lungs (LPS). In the case of use as an imaging agent, we evaluated the radiolabeled Fucoidan with 99mTc in inflamed lungs (LPS). The results demonstrated that Fucoidan has a therapeutic anti-inflammatory effect, especially in the lung model (LPS). Additionally, the imaging application demonstrated that radiolabeled Fucoidan (99mTc-Fucoidan) has an important chemoattraction for inflammation sites with very high bioaccumulation, which permits to think in an imaging application.

炎症是一种在多个层面上扰乱体内平衡的现象,其来源有许多,如感染、损伤和暴露于污染物。需要有效治疗炎症过程的新产品,因为可以选择性地成像炎症部位是一个全球性的问题。在这项研究中,我们评估了岩藻糖聚糖作为治疗和显像剂的适用性。我们已经评估了岩藻聚糖在两种炎症模型中的治疗目的:关节炎和肺(LPS)。在使用显像剂的情况下,我们评估了99mTc在炎症肺(LPS)中的放射性标记岩藻糖聚糖。结果表明岩藻多糖具有治疗性抗炎作用,特别是对肺模型(LPS)。此外,成像应用表明,放射性标记的岩藻多糖(99mtc -岩藻多糖)对具有很高生物积累的炎症部位具有重要的化学吸引力,这允许在成像应用中进行思考。
{"title":"Using pure Fucoidan and radiolabeled Fucoidan (99mTc-Fucoidan) as a new agent for inflammation diagnosis and therapy","authors":"Bianca Costa ,&nbsp;Luana Barbosa Corrêa ,&nbsp;Patrícia MachadoRodrigues e Silva ,&nbsp;Yago Amigo Pinho Jannini de Sá ,&nbsp;Fernanda Verdini Guimarães ,&nbsp;Luciana Magalhães Rebelo Alencar ,&nbsp;Rafael Loureiro Simões ,&nbsp;Edward Helal-Neto ,&nbsp;Eduardo Ricci-Junior ,&nbsp;Maria das Graças Muller de Oliveira Henriques ,&nbsp;Elaine Cruz Rosas ,&nbsp;Ralph Santos-Oliveira","doi":"10.1016/j.fhfh.2021.100049","DOIUrl":"https://doi.org/10.1016/j.fhfh.2021.100049","url":null,"abstract":"<div><p>Inflammation is a phenomenon responsible for the perturbation of homeostasis in several levels, with many sources, such as infection, injury, and exposure to contaminants. The necessity of new products that are effective in treating inflammation processes as can selectively imaging an inflammation site is a global issue. In this study, we have evaluated the applicability of Fucoidan as a therapeutic and imaging agent. We have assessed the Fucoidan in two inflammation models for therapeutic purposes: arthritis and lungs (LPS). In the case of use as an imaging agent, we evaluated the radiolabeled Fucoidan with 99mTc in inflamed lungs (LPS). The results demonstrated that Fucoidan has a therapeutic anti-inflammatory effect, especially in the lung model (LPS). Additionally, the imaging application demonstrated that radiolabeled Fucoidan (99mTc-Fucoidan) has an important chemoattraction for inflammation sites with very high bioaccumulation, which permits to think in an imaging application.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"2 ","pages":"Article 100049"},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025921000418/pdfft?md5=e854c97104d3df20c7fe14a4e555473b&pid=1-s2.0-S2667025921000418-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91979105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of emulsification/internal gelation-based microencapsulation on the viability of Akkermansia muciniphila upon prolonged storage and simulated gastrointestinal passage 乳化/内凝胶微胶囊化对嗜粘阿克曼氏菌长时间储存和模拟胃肠道生存能力的影响
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100084
Diana Almeida , Daniela Machado , Sérgio Sousa , Catarina Leal Seabra , Joana Cristina Barbosa , José Carlos Andrade , Ana Maria Gomes , Ana Cristina Freitas

Akkermansia muciniphila is a common human intestinal commensal with a mucin-degrading nature. Its immunomodulatory characteristics and regulatory role of mucus layer and gut barrier integrity highlight the potential benefits of using this bacterium as an interventional player against inflammatory/cardio-metabolic disorders. In this work, we evaluate the effect of microencapsulation by the emulsification/internal gelation method on A. muciniphila survival during aerobic storage (0, 15, 30 and 95 days) and subsequent exposure to simulated gastrointestinal passage, in comparison with that of free cells. The present results show that microencapsulation by internal gelation promotes a 64.4 % entrapment efficacy of A. muciniphila cells (maintaining a 108 order of magnitude for cell viability). Moreover, physical characterization showed that microparticles mean size was 53,5 ± 12,1 µm and, as observed by electron scanning microscopy, microcapsules were spherical in shape. More importantly, as storage time increased, encapsulated A. muciniphila demonstrated higher stability in GI conditions, when compared to its free counterpart. In conclusion, microencapsulation by internal gelation seems to be an appropriate strategy in protecting A. muciniphila against the detrimental gastrointestinal transit after long periods of aerobic refrigerated storage.

嗜粘菌是一种常见的人类肠道共生菌,具有降解粘蛋白的性质。它的免疫调节特性和黏液层和肠道屏障完整性的调节作用突出了使用这种细菌作为炎症/心脏代谢紊乱的介入参与者的潜在益处。在这项工作中,我们评估了乳化/内凝胶法微胶囊化对嗜粘杆菌在有氧储存(0、15、30和95天)和随后暴露于模拟胃肠道中的存活的影响,并与自由细胞进行了比较。本研究结果表明,内部凝胶微胶囊化可使嗜粘杆菌细胞的包封效率提高64.4%(维持细胞活力108个数量级)。物理表征表明,微胶囊的平均粒径为53,5±12,1µm,电子扫描显微镜观察到微胶囊呈球形。更重要的是,随着储存时间的增加,包封的嗜粘杆菌在胃肠道条件下表现出更高的稳定性。总之,内部凝胶微胶囊化似乎是保护嗜粘杆菌在长时间有氧冷藏后免受有害胃肠道运输的一种适当策略。
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引用次数: 3
Nutritional and technological aspects of barley β-glucan enriched biscuits containing isomaltulose as sucrose replacer 以异麦芽糖代替蔗糖的大麦β-葡聚糖饼干的营养和工艺研究
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100060
Athina Lazaridou, Kali Kotsiou, Costas G. Biliaderis

The objective of this work was to evaluate the physicochemical, sensory and potential glycemic response properties of a biscuit (β-GB) containing barley β-glucan at 6% w/w, sufficient to satisfy the EFSA's health claims related to cholesterol lowering and fecal bulk increase, and isomaltulose as sweetener. The freshly prepared β-GB was softer (p < 0.05) than the control biscuit (formulated with sucrose and without β-glucan); differences in hardness between the two biscuit formulations diminished during storage. The glucose release kinetics, evaluated by an in vitro enzymic assay, showed that after 5 h of digestion, glucose release was significantly (p < 0.05) lower for the β-GB (16.4%) compared to the control (26.4%) or to several commercial products of similar composition, which were lacking the β-glucan as ingredient (22.7 – 33.7%). The extent of in vitro glucose released was negatively correlated (r = -0.72; p = 0.020) to the viscosity values of ‘physiological extracts’ enclosed in dialysis tubes after 5 h incubation, and to their total dietary fiber content (r = -0.79; p = 0.006) after 20 min of digestion. The fortified biscuits with β-glucan and isomaltulose were found to be satisfactory in terms of flavor, sweetness, and texture by a group of “diabetic individuals” who rated these products highly, with an average of 5.8, in a 7-point hedonic overall acceptability scale. The low extent of glucose released along with the sensory results suggested that isomaltulose containing biscuits fortified with β-glucans could simultaneously provide various health benefits and be highly acceptable by consumers with glucose metabolism disorders.

本研究的目的是评估含有6% w/w大麦β-葡聚糖的饼干(β-GB)的物理化学、感官和潜在的血糖反应特性,足以满足欧洲食品安全局关于降低胆固醇和增加粪便体积的健康声明,并将异麦芽糖作为甜味剂。新鲜制备的β-GB较软(p <0.05),高于对照饼干(添加蔗糖,不添加β-葡聚糖);两种饼干配方之间的硬度差异在储存过程中减小了。体外酶法测定葡萄糖释放动力学表明,消化5 h后,葡萄糖释放显著(p <0.05), β-葡聚糖的含量(16.4%)低于对照组(26.4%),也低于几种成分相似、不含β-葡聚糖的商品(22.7% - 33.7%)。体外葡萄糖释放程度呈负相关(r = -0.72;P = 0.020)与透析管中封闭5 h后的“生理提取物”粘度值和其总膳食纤维含量(r = -0.79;P = 0.006)。一组“糖尿病患者”发现,含有β-葡聚糖和异麦芽糖的强化饼干在风味、甜味和质地方面都令人满意,他们对这些产品的评价很高,在7分的享乐总体可接受度量表中,平均得分为5.8分。低葡萄糖释放量和感官结果表明,添加β-葡聚糖的含异麦芽糖饼干可以同时提供多种健康益处,并且可以被糖代谢紊乱的消费者高度接受。
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引用次数: 5
Insights on β-glucan as a prebiotic coadjuvant in the treatment of diabetes mellitus: A review β-葡聚糖作为益生元辅助药物治疗糖尿病的研究进展
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100056
Katia Sivieri , Sonia Mariza de Oliveira , Audrey de Souza Marquez , Jara Pérez-Jiménez , Susana Nogueira Diniz

The prevalence of Diabetes Mellitus and, particularly, type 2 diabetes (T2D) is increasing every year worldwide. Therefore, changed glucose homeostasis is associated with altered gut microbiota and with the development of type 2 diabetes mellitus (T2DM) and related complications. Among other concerns, an important aspect within this issue is the reversion of dysbiosis reported to be present in T2D patients, in which diet plays a key role, and particularly dietary fiber has shown a promising position. β-glucans are heterogeneous non-starch polysaccharides, constituted by D-glucose monomers linked through different β-glycosidic bonds, and changes in their structure or molecular weight affect the expressed biological properties. They appear in certain cereals, yeasts, or mushrooms and are widely known by their hypocholesterolemia effect. This study explored the current knowledge on the potential of β-glucans to modulate glucose homeostasis due to their prebiotic action, by performing a bibliometric analysis using the VOSviewer software and a narrative review. The bibliometric analysis showed that, despite the high number of references dealing with β-glucan and diabetes, there are few articles on glucans, diabetes, and intestinal dysbiosis. The detailed study on preclinical studies and clinical trials conducted during the last decade showed an improvement in glucose homeostasis due to β-glucan supplementation, studies on biochemical markers, and gut microbiota, and β-glucan are scarce. Nevertheless, existing data showed, both in animals and humans, a tendency towards an increase in beneficial bacteria and in the production of short-chain fat acids (SCFAs), particularly butyric acid. These aspects should be confirmed in the years to come to establish clear recommendations for β-glucan as a prebiotic coadjutant in the management of Diabetes Mellitus.

糖尿病的患病率,特别是2型糖尿病(T2D)在世界范围内每年都在增加。因此,葡萄糖稳态的改变与肠道菌群的改变以及2型糖尿病(T2DM)及相关并发症的发生有关。在其他问题中,这个问题的一个重要方面是,据报道,在t2dm患者中存在生态失调的逆转,其中饮食起着关键作用,特别是膳食纤维已显示出有希望的地位。β-葡聚糖是由d -葡萄糖单体通过不同的β-糖苷键连接而成的非淀粉多糖类,其结构或分子量的变化会影响其表达的生物学特性。它们出现在某些谷物、酵母或蘑菇中,并因其降低胆固醇血症的作用而广为人知。本研究通过使用VOSviewer软件进行文献计量学分析和叙述性回顾,探索了β-葡聚糖由于其益生元作用而调节葡萄糖稳态的潜力的现有知识。文献计量学分析显示,尽管有大量文献涉及β-葡聚糖和糖尿病,但关于葡聚糖、糖尿病和肠道生态失调的文章很少。近十年来的临床前研究和临床试验的详细研究表明,由于β-葡聚糖的补充,葡萄糖稳态的改善,对生化标志物,肠道微生物群和β-葡聚糖的研究很少。然而,现有数据显示,在动物和人类中,有益细菌和短链脂肪酸(SCFAs),特别是丁酸的生产都有增加的趋势。这些方面应该在未来的几年里得到证实,以建立β-葡聚糖作为治疗糖尿病的益生元辅助剂的明确建议。
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引用次数: 16
Oxidized high-amylose starch as pickering stabilizer for oil-in-water emulsion and delivery of bioactive compound 氧化高直链淀粉作为水包油乳剂的酸洗稳定剂及生物活性化合物的输送
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100104
Zhi-Hua Zhang, Zhi-Yu Gu, Meng-Fan Li, Shan Liang, Zhi Huang, Min-Hua Zong, Xiao-Ling Wu, Wen-Yong Lou

Polysaccharide based emulsions have received considerable attention due to their biodegradability, biocompatibility and food-safety. In this study, oxidized high-amylose starch (OHAS) is adopted for stabilization of oil-in-water (O/W) emulsion. Results showed that OHAS with oxidation degree of 90% (DO90-OHAS) exhibited higher wettability with three-phase contact angle of 70.6°. The obtained emulsion was stable within a wide range of different solution condition, with pH ranging from 3 to 7, salt concentration as high as 1.0 M and temperature ranging from -25 to 80 ℃. The gel-like network structure of DO90-OHAS effectively stabilized the emulsion and prevented the aggregation of oil droplets under different pH, as evidenced by confocal laser scanning microscopy (CLSM) observation. Furthermore, DO90-OHAS based emulsion prepared at different pH showed satisfactory long-term storage stability (up to 30 days). The fabricated emulsion was utilized for encapsulation of hydrophobic bioactive substances, with ∼72.5% encapsulation efficiency for β-carotene. The DO90-OHAS based emulsion enabled the controlled-release of β-carotene in vitro, with antioxidant activity maintained ∼50% of initial activity when exposed to elevated temperature (80°C). This research may provide a new avenue for OHAS based Pickering emulsions preparation and bioactive components delivery.

多糖基乳剂因其生物可降解性、生物相容性和食品安全性而受到广泛关注。本研究采用氧化高直链淀粉(OHAS)稳定水包油(O/W)乳液。结果表明,氧化度为90%的OHAS (DO90-OHAS)具有较高的润湿性,三相接触角为70.6°。在pH为3 ~ 7、盐浓度为1.0 M、温度为-25 ~ 80℃的不同溶液条件下,所制得的乳状液具有较好的稳定性。共聚焦激光扫描显微镜(CLSM)观察表明,DO90-OHAS的凝胶状网络结构在不同pH下都能有效地稳定乳液,防止油滴聚集。此外,在不同pH下制备的DO90-OHAS基乳液表现出令人满意的长期储存稳定性(长达30天)。制备的乳液用于疏水生物活性物质的包封,β-胡萝卜素包封率为~ 72.5%。基于DO90-OHAS的乳液能够在体外控制β-胡萝卜素的释放,当暴露于高温(80℃)时,其抗氧化活性保持在初始活性的50%左右。本研究为OHAS基皮克林乳剂的制备及生物活性成分的传递提供了新的途径。
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引用次数: 1
Enzymatic modification of Fish Gelatin and Beet Pectin using Horseradish peroxidase 用辣根过氧化物酶对鱼明胶和甜菜果胶进行酶修饰
IF 1.9 Q1 CHEMISTRY, APPLIED Pub Date : 2022-12-01 DOI: 10.1016/j.fhfh.2022.100080
Ebenezer Asiamah , Dominic Aboagye , Ahmed A. Zaky , Charles Asakiya , Ethel Juliet Serwa Blessie

The Fish Gelatin (FG), a good alternative for unhealthy and limited socio-cultural mammalian gelatin appears to possess endogenous structural limitations. The goal of this work was to use enzymatic crosslinking to modify cold-water Fish Gelatin (FG) with Beet Pectin. Reaction conditions were optimized by a single factorial experiment and covalent crosslinking was measured by ultraviolet (UV)-Vis spectroscopy at 340 nm to indicate Horseradish Peroxidase (HRP) catalyzes Beet Pectin (BP). At 50 °C for 4 h, the highest weight ratio of heterologous adducts between FG-BP was 1:3, with HRP and Hydrogen peroxide (H2O2) of 2 µg/mL and 0.067%, (v/v), respectively. Intermolecular cross-linking was found between treated samples using ATR-FTIR and Sodium Dodecyl Sulphur and Polyacrylamide Gel Electrophoresis (SDS-PAGE). The heterologous product, control FG, and BP as well as a mixture of untreated FG-BP had a β-sheet of 41.14%, 39.65%, 39.9%, and 40.0%, respectively. The maximum reduction in elution was obtained in heterogeneous FG-BP complex. Furthermore, a schematic mechanism for Cold-water Fish Gelatin and Beet Pectin was proposed. Overall, peroxidase crosslinked BP was able to modify cold-water Fish Gelatin. The use of Horseradish peroxidase on Fish Gelatin could provide a practical way of building the FG-BP complex as a basis for understanding the FG functionalities comprehensively.

鱼类明胶(FG),一个很好的替代不健康和有限的社会文化哺乳动物明胶似乎具有内源性结构限制。本研究的目的是利用酶交联技术,用甜菜果胶改性冷水鱼明胶。通过单因子实验优化反应条件,并在340 nm紫外-可见光谱下测定共价交联反应,证实辣根过氧化物酶(HRP)催化甜菜果胶(BP)。在50℃作用4 h时,FG-BP间外源加合物的最大重量比为1:3,HRP和过氧化氢(H2O2)分别为2µg/mL和0.067% (v/v)。利用ATR-FTIR、十二烷基硫钠和聚丙烯酰胺凝胶电泳(SDS-PAGE)发现处理后的样品之间存在分子间交联。异种产物、对照FG和BP以及未处理FG-BP的混合物的β-sheet分别为41.14%、39.65%、39.9%和40.0%。在非均相FG-BP络合物中洗脱效果最大。并对冷水鱼明胶和甜菜果胶的合成机理进行了初步探讨。总的来说,过氧化物酶交联BP能够修饰冷水鱼明胶。辣根过氧化物酶在鱼明胶上的应用为FG- bp复合物的构建提供了一种实用的方法,为全面了解FG的功能奠定了基础。
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引用次数: 2
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Food Hydrocolloids for Health
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