Pub Date : 2025-01-16DOI: 10.1016/j.fhfh.2025.100196
Xingzhu Wu , Wallace Yokoyama , Yuqing Tan , Glenn Bartley , Ling Chen , James Pan , Priscila Alves Buongiorno , Jose Berrios , Tara McHugh , Zhongli Pan
Pomegranate peel waste is a byproduct of juice processing and 1.6 million tons are produced globally each year. Pomegranate peels have a high dietary fiber content and unique polyphenol profile suggesting it may have health benefits. This study aimed to investigate the hypocholesterolemic and anti-obesity effects of pomegranate peel powder (PPP) and water extract (PPE) in Golden Syrian hamsters fed with high-fat (39 % fat calorie, HF) diets. Hamsters were fed either the HF, or the HF diet supplemented with 2.5 % or 5 % PPE (LE and HE, respectively), or 5 % or 10 % PPP (low-peel (LP) and high-peel (HP), respectively. After 4 weeks of feeding, hamsters gained 16.10- 33.82 g of weight and the feeding efficacy ranged from 0.10 to 0.18. The HP group had the lowest weight gain and feed efficacy while others were not significantly different. The HP group had significantly lower liver-to-body weight ratio (3.10 ± 0.08 % vs 3.65 ± 0.09 %), fasting blood glucose (68.11 ± 5.27 vs 82.94 ± 6.49 mg/dL), and hepatic lipid content (6.31 ± 0.26 vs 7.49 ± 0.22 g/100 g liver) compared to the HF group. PPP ingestion significantly increased LDL but decreased triglycerides. PPP and PPE feeding resulted in microbiota phyla Firmicutes-to-Bacteroidetes ratio characteristic of leaner phenotypes. HMG-CoAR and LDLR expression were reduced, suggesting that decreased uptake of LDL was not sufficient to lower plasma LDL, even with reduced cholesterol synthesis.
石榴皮废料是果汁加工的副产品，全球每年产生160万吨。石榴皮含有很高的膳食纤维含量和独特的多酚成分，这表明石榴皮可能对健康有益。本研究旨在探讨石榴皮粉（PPP）和水提物（PPE）对高脂肪（39%脂肪热量，HF）饲粮喂养的叙利亚金仓鼠的降胆固醇和抗肥胖作用。分别饲喂HF、HF饲粮中添加2.5%或5% PPE（分别为LE和HE）、5%或10% PPP（分别为低削皮（LP）和高削皮（HP））的仓鼠。饲喂4周后，仓鼠增重16.10 ~ 33.82 g，饲喂效率为0.10 ~ 0.18。HP组的增重和饲料效率最低，其他组差异不显著。与HF组相比，HP组的肝体重比（3.10±0.08% vs 3.65±0.09%）、空腹血糖（68.11±5.27 vs 82.94±6.49 mg/dL）和肝脏脂质含量（6.31±0.26 vs 7.49±0.22 g/100 g肝脏）均显著降低。PPP摄入显著增加LDL，但降低甘油三酯。PPP和PPE饲养导致厚壁菌门与拟杆菌门的微生物比例呈较瘦表型特征。HMG-CoAR和LDLR表达降低，表明LDL摄取减少并不足以降低血浆LDL，即使胆固醇合成减少。
{"title":"Pomegranate peel powder and extract improved weight control, lipid metabolism and gut microbiota in hamsters fed with standard american diets","authors":"Xingzhu Wu , Wallace Yokoyama , Yuqing Tan , Glenn Bartley , Ling Chen , James Pan , Priscila Alves Buongiorno , Jose Berrios , Tara McHugh , Zhongli Pan","doi":"10.1016/j.fhfh.2025.100196","DOIUrl":"10.1016/j.fhfh.2025.100196","url":null,"abstract":"<div><div>Pomegranate peel waste is a byproduct of juice processing and 1.6 million tons are produced globally each year. Pomegranate peels have a high dietary fiber content and unique polyphenol profile suggesting it may have health benefits. This study aimed to investigate the hypocholesterolemic and anti-obesity effects of pomegranate peel powder (PPP) and water extract (PPE) in Golden Syrian hamsters fed with high-fat (39 % fat calorie, HF) diets. Hamsters were fed either the HF, or the HF diet supplemented with 2.5 % or 5 % PPE (LE and HE, respectively), or 5 % or 10 % PPP (low-peel (LP) and high-peel (HP), respectively. After 4 weeks of feeding, hamsters gained 16.10- 33.82 g of weight and the feeding efficacy ranged from 0.10 to 0.18. The HP group had the lowest weight gain and feed efficacy while others were not significantly different. The HP group had significantly lower liver-to-body weight ratio (3.10 ± 0.08 % vs 3.65 ± 0.09 %), fasting blood glucose (68.11 ± 5.27 vs 82.94 ± 6.49 mg/dL), and hepatic lipid content (6.31 ± 0.26 vs 7.49 ± 0.22 g/100 g liver) compared to the HF group. PPP ingestion significantly increased LDL but decreased triglycerides. PPP and PPE feeding resulted in microbiota phyla <em>Firmicutes</em>-<em>to</em>-<em>Bacteroidetes</em> ratio characteristic of leaner phenotypes. HMG-CoAR and LDLR expression were reduced, suggesting that decreased uptake of LDL was not sufficient to lower plasma LDL, even with reduced cholesterol synthesis.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100196"},"PeriodicalIF":4.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1016/j.fhfh.2025.100197
Johan Mendoza , Omar Peñuñuri-Miranda , María d.C. Valdez-Cárdenas , Carmen O. Melendez-Pizarro , Daniel Lardizabal-Gutiérrez , Francisco Paraguay-Delgado , Armando Quintero-Ramos
This study evaluates the effects of pH levels and Zein/Gum Arabic (Z/GA) ratios on key encapsulation parameters of an anthocyanin-rich extract (ARE) derived from purple corn cob, using the antisolvent precipitation method. Parameters analyzed include encapsulation efficiencies for anthocyanins (%AEE) and polyphenols (%PEE), nanoparticle size, polydispersity index, ζ-potential, and thermal stability at 80 and 180 °C. Particles without GA showed poor stability and low %AEE, particularly at lower pH. Conversely, the addition of GA significantly enhanced encapsulation efficiency, especially under acidic conditions (pH 2–4), and improved the particle size uniformity. At Z/GA ratio of 1:1, GA played a crucial role in stabilizing nanoparticles, effectively preventing aggregation even when the net particle charge was near to zero. Characterization by SEM, FTIR and TGA confirmed the morphological, structural, and thermal properties of the encapsulated particles. Thermal stability tests demonstrated that encapsulated anthocyanins exhibited significantly improved resistance to thermal degradation, with half-life extended up to threefold compared to unencapsulated counterparts. These results highlight the potential of encapsulating ARE from purple corn cob in Z/GA matrices as a method to preserve anthocyanins functionality, improve their thermal stability during food processing, and enhance application in food systems. Additionally, this approach offers a sustainable alternative, adding value to agricultural by-products and promoting waste valorization in the food industry. However, further research on scalability, cost-effectiveness, and application in food processing systems are needed.
{"title":"Encapsulation of anthocyanins from purple corn cob via antisolvent precipitation: Effect of pH and zein/gum arabic ratio on the antioxidant activity, particle size and thermal stability","authors":"Johan Mendoza , Omar Peñuñuri-Miranda , María d.C. Valdez-Cárdenas , Carmen O. Melendez-Pizarro , Daniel Lardizabal-Gutiérrez , Francisco Paraguay-Delgado , Armando Quintero-Ramos","doi":"10.1016/j.fhfh.2025.100197","DOIUrl":"10.1016/j.fhfh.2025.100197","url":null,"abstract":"<div><div>This study evaluates the effects of pH levels and Zein/Gum Arabic (Z/GA) ratios on key encapsulation parameters of an anthocyanin-rich extract (ARE) derived from purple corn cob, using the antisolvent precipitation method. Parameters analyzed include encapsulation efficiencies for anthocyanins (%AEE) and polyphenols (%PEE), nanoparticle size, polydispersity index, ζ-potential, and thermal stability at 80 and 180 °C. Particles without GA showed poor stability and low %AEE, particularly at lower pH. Conversely, the addition of GA significantly enhanced encapsulation efficiency, especially under acidic conditions (pH 2–4), and improved the particle size uniformity. At Z/GA ratio of 1:1, GA played a crucial role in stabilizing nanoparticles, effectively preventing aggregation even when the net particle charge was near to zero. Characterization by SEM, FTIR and TGA confirmed the morphological, structural, and thermal properties of the encapsulated particles. Thermal stability tests demonstrated that encapsulated anthocyanins exhibited significantly improved resistance to thermal degradation, with half-life extended up to threefold compared to unencapsulated counterparts. These results highlight the potential of encapsulating ARE from purple corn cob in Z/GA matrices as a method to preserve anthocyanins functionality, improve their thermal stability during food processing, and enhance application in food systems. Additionally, this approach offers a sustainable alternative, adding value to agricultural by-products and promoting waste valorization in the food industry. However, further research on scalability, cost-effectiveness, and application in food processing systems are needed.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100197"},"PeriodicalIF":4.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to develop a novel, degradable antimicrobial bioactive coating by combining Ferula haussknechtii gum and polyethylene oxide with lemongrass essential oil (LGEO) using the emulsion electrospinning technique. The LGEO emulsion in the F. haussknechtii gum/polyethylene oxide solution was prepared via ultrasonic method, and its physicochemical properties, including pH, electrical conductivity, density, and apparent viscosity, were systematically evaluated. The microstructural morphology of the electrospun coating was analyzed using scanning electron microscopy (SEM). The antimicrobial properties and antioxidant potential of the active electrospun coating were also assessed. The resulting electrospun fibers had an average diameter of 0.56 μm and contained over 36 bioactive compounds, exhibiting radical scavenging activity of approximately 74.51 %. The LGEO was incorporated into the bioactive coating at concentrations of 3, 6, and 9 % (v/v). The antimicrobial efficacy of the electrospun coating was tested against Gram-positive Gram-negative, and Aspergillus niger. The highest antimicrobial activity was observed with the electrospun coating containing 9 % LGEO. The results revealed that increasing the LGEO concentration in the emulsion resulted in decreased pH, apparent viscosity, and density, while electrical conductivity increased. SEM analysis confirmed the formation of uniform, bead-free electrospun fibers across all LGEO concentrations. FTIR analysis validated the successful incorporation of emulsified LGEO into the electrospun fibers. These findings demonstrate that the inclusion of LGEO in bioactive edible coatings can significantly enhance antimicrobial protection, particularly for minimally processed foods, while potentially extending shelf life by reducing microbial contamination.
{"title":"Emulsion electrospinning of lemongrass essential Oil-Loaded Ferula haussknechtii gum/ Polyethylene oxide as bioactive coating","authors":"Saeedeh Jafari, Abdollah Hematian Sourki, Safoora Pashangeh","doi":"10.1016/j.fhfh.2025.100195","DOIUrl":"10.1016/j.fhfh.2025.100195","url":null,"abstract":"<div><div>This study aimed to develop a novel, degradable antimicrobial bioactive coating by combining <em>Ferula haussknechtii</em> gum and polyethylene oxide with lemongrass essential oil (LGEO) using the emulsion electrospinning technique. The LGEO emulsion in the <em>F. haussknechtii</em> gum/polyethylene oxide solution was prepared via ultrasonic method, and its physicochemical properties, including pH, electrical conductivity, density, and apparent viscosity, were systematically evaluated. The microstructural morphology of the electrospun coating was analyzed using scanning electron microscopy (SEM). The antimicrobial properties and antioxidant potential of the active electrospun coating were also assessed. The resulting electrospun fibers had an average diameter of 0.56 μm and contained over 36 bioactive compounds, exhibiting radical scavenging activity of approximately 74.51 %. The LGEO was incorporated into the bioactive coating at concentrations of 3, 6, and 9 % (v/v). The antimicrobial efficacy of the electrospun coating was tested against Gram-positive Gram-negative<em>,</em> and <em>Aspergillus niger</em>. The highest antimicrobial activity was observed with the electrospun coating containing 9 % LGEO. The results revealed that increasing the LGEO concentration in the emulsion resulted in decreased pH, apparent viscosity, and density, while electrical conductivity increased. SEM analysis confirmed the formation of uniform, bead-free electrospun fibers across all LGEO concentrations. FTIR analysis validated the successful incorporation of emulsified LGEO into the electrospun fibers. These findings demonstrate that the inclusion of LGEO in bioactive edible coatings can significantly enhance antimicrobial protection, particularly for minimally processed foods, while potentially extending shelf life by reducing microbial contamination.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100195"},"PeriodicalIF":4.6,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-20DOI: 10.1016/j.fhfh.2024.100193
Varanya Techasukthavorn , Jirarat Anuntagool
Thickened fluids, or dysphagic drinks, are commonly prescribed for individuals with swallowing difficulties. They help slow down food bolus in the swallowing process, reducing the risk of choking and aspiration. The main goal of this study was to create a set of high-protein supplement drinks varying in caloric density level. Besides, the study aimed to examine both shear and extensional rheological properties along with visual cohesiveness. Soy protein-based formulations A to F were developed with caloric densities: 1, 1.2, 1.5, 2, 2.5, and 3 kcal/mL, respectively. Each formulation was assessed through the International Dysphagia Diet Standardisation Initiative (IDDSI) flow test, shear and extensional rheological behavior, and visual cohesiveness. All samples exhibited shear-thinning behavior with viscosity increased with higher caloric density. Formulations A and B were classified as low-viscosity liquids, while formulations C, D, E, and F were suitable for extensional flow measurements with extensional properties improving as density increased. Visual cohesiveness, assessed through elongation shape and flow behavior, enhances with formulation concentration. Formulations A and B may require the addition of thickening agents to serve as thickened nutritional supplements for dysphagia management. Formulations C, D, and E were mildly to moderately thick, whereas formulation F, characterized by extremely thick with very high yield stress, may cause multiple swallows. These findings highlight the potential for developing higher-calorie supplements without thickeners as a practical strategy to enhance energy and protein intake in individuals with dysphagia. To ensure safe swallowing, future research should validate these results using in-vitro throat models and clinical studies.
{"title":"Rheological properties and visual cohesiveness of soy protein-based formulations without thickening agents in dysphagic management","authors":"Varanya Techasukthavorn , Jirarat Anuntagool","doi":"10.1016/j.fhfh.2024.100193","DOIUrl":"10.1016/j.fhfh.2024.100193","url":null,"abstract":"<div><div>Thickened fluids, or dysphagic drinks, are commonly prescribed for individuals with swallowing difficulties. They help slow down food bolus in the swallowing process, reducing the risk of choking and aspiration. The main goal of this study was to create a set of high-protein supplement drinks varying in caloric density level. Besides, the study aimed to examine both shear and extensional rheological properties along with visual cohesiveness. Soy protein-based formulations A to F were developed with caloric densities: 1, 1.2, 1.5, 2, 2.5, and 3 kcal/mL, respectively. Each formulation was assessed through the International Dysphagia Diet Standardisation Initiative (IDDSI) flow test, shear and extensional rheological behavior, and visual cohesiveness. All samples exhibited shear-thinning behavior with viscosity increased with higher caloric density. Formulations A and B were classified as low-viscosity liquids, while formulations C, D, E, and F were suitable for extensional flow measurements with extensional properties improving as density increased. Visual cohesiveness, assessed through elongation shape and flow behavior, enhances with formulation concentration. Formulations A and B may require the addition of thickening agents to serve as thickened nutritional supplements for dysphagia management. Formulations C, D, and E were mildly to moderately thick, whereas formulation F, characterized by extremely thick with very high yield stress, may cause multiple swallows. These findings highlight the potential for developing higher-calorie supplements without thickeners as a practical strategy to enhance energy and protein intake in individuals with dysphagia. To ensure safe swallowing, future research should validate these results using in-vitro throat models and clinical studies.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"7 ","pages":"Article 100193"},"PeriodicalIF":4.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we analyzed the absorption of linear and cyclic dipeptides containing Gly, Pro, Ala, and Val by human blood following the ingestion of elastin hydrolysate. As in previous studies, Pro-Gly was transferred into blood at the highest concentration (Cmax; 14.63 nmol/mL). Moreover, this is the first study to show that Gly-Pro, Pro-Ala, Gly-Ala, cyclo(Gly-Pro), cyclo(Pro-Ala), cyclo(Pro-Val) and cyclo(Gly-Ala) also increase in blood after the ingestion of elastin hydrolysate. The contents of these cyclic dipeptides, which amounts in elastin hydrolysate is verry small, suggested that elastin digestives may be cyclized during digestion and absorption by human blood following the ingestion of elastin hydrolysate. This study suggested that these blood-transferrable linear and cyclic dipeptides could be candidates for elastin-derived bioactive peptides, and this finding consequently led to the further experiments that has been required for clarifying the bioactivities and mechanisms of beneficial effects of elastin hydrolysate.
{"title":"Increase in blood-transferable linear and cyclic dipeptides in human plasma following ingestion of elastin hydrolysate","authors":"Yu Iwasaki , Mikako Sato , Yoshinori Katakura , Yukihiro Sugawara , Yasutaka Shigemura","doi":"10.1016/j.fhfh.2024.100188","DOIUrl":"10.1016/j.fhfh.2024.100188","url":null,"abstract":"<div><div>In this study, we analyzed the absorption of linear and cyclic dipeptides containing Gly, Pro, Ala, and Val by human blood following the ingestion of elastin hydrolysate. As in previous studies, Pro-Gly was transferred into blood at the highest concentration (Cmax; 14.63 nmol/mL). Moreover, this is the first study to show that Gly-Pro, Pro-Ala, Gly-Ala, cyclo(Gly-Pro), cyclo(Pro-Ala), cyclo(Pro-Val) and cyclo(Gly-Ala) also increase in blood after the ingestion of elastin hydrolysate. The contents of these cyclic dipeptides, which amounts in elastin hydrolysate is verry small, suggested that elastin digestives may be cyclized during digestion and absorption by human blood following the ingestion of elastin hydrolysate. This study suggested that these blood-transferrable linear and cyclic dipeptides could be candidates for elastin-derived bioactive peptides, and this finding consequently led to the further experiments that has been required for clarifying the bioactivities and mechanisms of beneficial effects of elastin hydrolysate.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"6 ","pages":"Article 100188"},"PeriodicalIF":4.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.fhfh.2024.100190
Joy J. Adeyemi , Abayomi M. Ajayi , Tolulope O. Ajala
Phyllantus amarus and P. muellerianus are herbs reported to possess anti-inflammatory activity. Antinociceptive and anti-inflammatory activities of alginate-based microsphere loaded with extracts of P. amarus and P. muellerianus was here reported. The extract-loaded microspheres were prepared using the ionotropic gelation method. The particle size, swelling index, entrapment efficiency, and FTIR spectroscopy were determined. Antinociceptive and anti-inflammatory activities of extract-loaded microspheres were evaluated in hotplate-induced nociception in mice and carrageenan-induced rat paw oedema, respectively. The physicohemical results showed rigid, free-flowing and spherical microspheres, with particle sizes ranging from 985.977±13.65 to 1232±12.99 µm and entrapment efficiencies of 20.9 ± 0.341 to 45.0 ± 0.002 %. Polymer-drug interaction revealed bands indicating aromatics, alcohols and alkenes. The extract-loaded microspheres showed improved antinociceptive and anti-inflammatory activities when compared to the extracts alone. The sodium alginate-based microspheres loaded with Phyllanthus amarus and Phyllanthus muellerianus extracts showed acceptable physicochemical properties and had improved antinociceptive and anti-inflammatory activity compared to the extracts alone.
{"title":"Alginate-based microencapsulation enhances antinociceptive and anti-inflammatory activities of Phyllanthus amarus and Phyllanthus muellerianus","authors":"Joy J. Adeyemi , Abayomi M. Ajayi , Tolulope O. Ajala","doi":"10.1016/j.fhfh.2024.100190","DOIUrl":"10.1016/j.fhfh.2024.100190","url":null,"abstract":"<div><div><em>Phyllantus amarus</em> and <em>P. muellerianus</em> are herbs reported to possess anti-inflammatory activity. Antinociceptive and anti-inflammatory activities of alginate-based microsphere loaded with extracts of <em>P. amarus</em> and <em>P. muellerianus</em> was here reported. The extract-loaded microspheres were prepared using the ionotropic gelation method. The particle size, swelling index, entrapment efficiency, and FTIR spectroscopy were determined. Antinociceptive and anti-inflammatory activities of extract-loaded microspheres were evaluated in hotplate-induced nociception in mice and carrageenan-induced rat paw oedema, respectively. The physicohemical results showed rigid, free-flowing and spherical microspheres, with particle sizes ranging from 985.977±13.65 to 1232±12.99 µm and entrapment efficiencies of 20.9 ± 0.341 to 45.0 ± 0.002 %. Polymer-drug interaction revealed bands indicating aromatics, alcohols and alkenes. The extract-loaded microspheres showed improved antinociceptive and anti-inflammatory activities when compared to the extracts alone. The sodium alginate-based microspheres loaded with <em>Phyllanthus amarus</em> and <em>Phyllanthus muellerianus</em> extracts showed acceptable physicochemical properties and had improved antinociceptive and anti-inflammatory activity compared to the extracts alone.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"6 ","pages":"Article 100190"},"PeriodicalIF":4.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.fhfh.2024.100191
Maria Lilibeth Manzanilla-Valdez , Christine Boesch , Cristina Martinez-Villaluenga , Sarita Montaño , Alan Javier Hernández-Álvarez
Quinoa (Chenopodium quinoa Willd) has gained popularity as a plant-based protein source due to its high protein content and complete amino acid profile. However, protein extraction methods such as alkaline solubilization coupled to isoelectric precipitation (ASIP), can affect protein structure, digestibility, nutritional quality, and the composition of antinutritional factors. This study aimed to assess the effects of ASIP on the secondary structure, protein quality and antinutritional factors (ANFs) composition from three quinoa varieties. The results showed that quinoa protein isolates exhibited a decrease in random coil structures, while β-turns and β-sheets increased, as indicated by FTIR analysis. In vitro protein digestibility improved after protein extraction, ranging from 82.12% to 84.50%. The amino acid score ranged from 0.67 – 0.88, with Yellow quinoa protein concentrate exhibiting the highest value. Black quinoa protein isolate showed the lowest total oxalate content (105.00 mg/100g), while Red quinoa protein concentrate presented higher levels of phytic acid (2.0 g/100 g), saponins (150.0 mg/g), and total phenolic compounds (161.5 mg GAE/100g). Notably, gluten content decreased in all samples following protein extraction. Despite the presence of certain ANFs in quinoa protein isolates/concentrates, the protein quality of quinoa isolates and concentrates was not adversely affected. In conclusion, the extraction process reduced several ANFs, including lectins, oxalates, and gluten, while enhancing the overall protein quality.
{"title":"“Enhancing quinoa (Chenopodium quinoa Willd) protein extraction: Alkaline solubilization coupled to isoelectric precipitation effects on structure, digestibility and antinutrients”","authors":"Maria Lilibeth Manzanilla-Valdez , Christine Boesch , Cristina Martinez-Villaluenga , Sarita Montaño , Alan Javier Hernández-Álvarez","doi":"10.1016/j.fhfh.2024.100191","DOIUrl":"10.1016/j.fhfh.2024.100191","url":null,"abstract":"<div><div>Quinoa (<em>Chenopodium quinoa</em> Willd) has gained popularity as a plant-based protein source due to its high protein content and complete amino acid profile. However, protein extraction methods such as alkaline solubilization coupled to isoelectric precipitation (ASIP), can affect protein structure, digestibility, nutritional quality, and the composition of antinutritional factors. This study aimed to assess the effects of ASIP on the secondary structure, protein quality and antinutritional factors (ANFs) composition from three quinoa varieties. The results showed that quinoa protein isolates exhibited a decrease in random coil structures, while β-turns and β-sheets increased, as indicated by FTIR analysis. <em>In vitro</em> protein digestibility improved after protein extraction, ranging from 82.12% to 84.50%. The amino acid score ranged from 0.67 – 0.88, with Yellow quinoa protein concentrate exhibiting the highest value. Black quinoa protein isolate showed the lowest total oxalate content (105.00 mg/100g), while Red quinoa protein concentrate presented higher levels of phytic acid (2.0 g/100 g), saponins (150.0 mg/g), and total phenolic compounds (161.5 mg GAE/100g). Notably, gluten content decreased in all samples following protein extraction. Despite the presence of certain ANFs in quinoa protein isolates/concentrates, the protein quality of quinoa isolates and concentrates was not adversely affected. In conclusion, the extraction process reduced several ANFs, including lectins, oxalates, and gluten, while enhancing the overall protein quality.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"6 ","pages":"Article 100191"},"PeriodicalIF":4.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.fhfh.2024.100189
Samaa Abdullah , Nabil A. Alhakamy , Hatim S. AlKhatib , Rana Abu Huwaij , Hadil Alahdal , Abeer A. Altamimi
Vortioxetine (VTX) is a new atypical antidepressant used to treat major depression and other mental disorders. Due to its low water solubility, oral absorption, and fast metabolism, VTX has been commercially manufactured and sold as a hydrobromide. Long-term VTX hydrobromide therapy is frequently associated with respiratory irritation and digestive dysfunction. Two techniques were developed for dissolution, swelling, adherence, and penetration enhancements. The techniques were the VTX and casein (CAS) complexation using the maximum loading capacity, and VTX-polymeric nano micelle using the “Sandwich Technique”. This study includes the maximum VTX-CAS binding capacity determination, VTX-CAS complex preparation, polymeric nano micelle encapsulating VTX-CAS complex optimizations, physiochemical characterisations, solubility assessment, VTX release analysis, swelling analysis and mucus-penetrating study of the VTX-CAS complex and VTX polymeric nano micelle in comparison to the VTX raw material. The optimum VTX-polymeric nano micelle dissolution, swelling, adherence, and penetration enhancements were supported by the results of 91.10±16.34 nm, +19 mV zeta-potential, structural arrangements, and enhanced amorphic character with the morphology and size distribution (50–100 nm). The VTX-polymeric nano micelle could serve as an oral alternative to the VTX hydrobromide therapy based on the results of the biocompatibility and in vivo absorption studies for the VTX-polymeric nano micellar system.
{"title":"Casein and acryl amide complexation and bio-adhesive polymeric nano micelles influence on vortioxetine dissolution, penetration enhancement and in vivo absorption","authors":"Samaa Abdullah , Nabil A. Alhakamy , Hatim S. AlKhatib , Rana Abu Huwaij , Hadil Alahdal , Abeer A. Altamimi","doi":"10.1016/j.fhfh.2024.100189","DOIUrl":"10.1016/j.fhfh.2024.100189","url":null,"abstract":"<div><div>Vortioxetine (VTX) is a new atypical antidepressant used to treat major depression and other mental disorders. Due to its low water solubility, oral absorption, and fast metabolism, VTX has been commercially manufactured and sold as a hydrobromide. Long-term VTX hydrobromide therapy is frequently associated with respiratory irritation and digestive dysfunction. Two techniques were developed for dissolution, swelling, adherence, and penetration enhancements. The techniques were the VTX and casein (CAS) complexation using the maximum loading capacity, and VTX-polymeric nano micelle using the “Sandwich Technique”. This study includes the maximum VTX-CAS binding capacity determination, VTX-CAS complex preparation, polymeric nano micelle encapsulating VTX-CAS complex optimizations, physiochemical characterisations, solubility assessment, VTX release analysis, swelling analysis and mucus-penetrating study of the VTX-CAS complex and VTX polymeric nano micelle in comparison to the VTX raw material. The optimum VTX-polymeric nano micelle dissolution, swelling, adherence, and penetration enhancements were supported by the results of 91.10±16.34 nm, +19 mV zeta-potential, structural arrangements, and enhanced amorphic character with the morphology and size distribution (50–100 nm). The VTX-polymeric nano micelle could serve as an oral alternative to the VTX hydrobromide therapy based on the results of the biocompatibility and <em>in vivo</em> absorption studies for the VTX-polymeric nano micellar system.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"6 ","pages":"Article 100189"},"PeriodicalIF":4.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-17DOI: 10.1016/j.fhfh.2024.100187
Andrés Felipe Alzate-Arbeláez , Farid B. Cortés , Benjamín A. Rojano
In this study, the nutraceutical properties of ethanolic extract of Hyeronima macrocarpa fruits, immobilized on nanocellulose-based aerogels (NCAG) synthesized from the seeds were studied. Specifically, bioactives with antioxidant properties of the pulp were determined, NCAG and homologs of acetate (NCAG-A) and sulfate (NCAG-S) were obtained, and characterized from the seed, the aerogels loaded with antioxidants were studied to determine the anti-radical activity, digestion patterns, protein oxidation inhibition, and toxicological properties. The berries presented a high anthocyanin content of 1317.4 mg C3G/100 g FW and ORAC value of 12,732 µmol Trolox/100 g FW, which make an important source of antioxidants. The seeds presented cellulose content of 61.4 % with a NC yield of 38.4 %. NCAG and their surface homologs were successfully synthesized and characterized by FTIR, DLS, and TEM finding the characteristic bands of the main functional groups, NC presented particle sizes ranging from 64 to 141 nm, BET analysis showed surface areas of 71.1, 102.3, and 183.5 m2/g for NCAG-A, NCAG, and NCAG-S, respectively, and pore sizes of 36–38 nm called mesopores. NCAG presented the highest capacity to trap reactive oxygen species (106.8 mg catechin Eq./g., 86.5 % OH• trapped, respectively). All samples showed the capacity to delay the oxidation of a protein system in a dose-dependent manner, with IC50 values of 70 mg/L (NCAG), 176.3 mg/L (NCAG-A), and 255.6 mg/L (NCAG-S). In vitro digestion showed that NCAG-S was more efficient in delivering anthocyanins under gastric conditions (bioaccessibility of 59.3 %), and NCAG under duodenal conditions (bioaccessibility of 88.2 %). The differences found in samples for the different functional assays can be explained by the various types of interactions generated between the antioxidant molecules and aerogels, in the various media where the analyses are carried out. The results indicate nanocellulose-based aerogels, synthesized from lignocellulosic residues of H. macrocarpa seeds, proved to be porous matrices capable of carrying bioactive substances, and presented interesting properties for the delivery and conservation of antioxidant molecules such as anthocyanins and other polyphenols, achieving an in vitro protective effect against the oxidation of biomolecules.
{"title":"Functional properties and toxicological analysis of nanocellulose-based aerogels loaded with polyphenols from Hyeronima macrocarpa berries","authors":"Andrés Felipe Alzate-Arbeláez , Farid B. Cortés , Benjamín A. Rojano","doi":"10.1016/j.fhfh.2024.100187","DOIUrl":"10.1016/j.fhfh.2024.100187","url":null,"abstract":"<div><div>In this study, the nutraceutical properties of ethanolic extract of <em>Hyeronima macrocarpa</em> fruits, immobilized on nanocellulose-based aerogels (NCAG) synthesized from the seeds were studied. Specifically, bioactives with antioxidant properties of the pulp were determined, NCAG and homologs of acetate (NCAG-A) and sulfate (NCAG-S) were obtained, and characterized from the seed, the aerogels loaded with antioxidants were studied to determine the anti-radical activity, digestion patterns, protein oxidation inhibition, and toxicological properties. The berries presented a high anthocyanin content of 1317.4 mg C3G/100 g FW and ORAC value of 12,732 µmol Trolox/100 g FW, which make an important source of antioxidants. The seeds presented cellulose content of 61.4 % with a NC yield of 38.4 %. NCAG and their surface homologs were successfully synthesized and characterized by FTIR, DLS, and TEM finding the characteristic bands of the main functional groups, NC presented particle sizes ranging from 64 to 141 nm, BET analysis showed surface areas of 71.1, 102.3, and 183.5 m<sup>2</sup>/g for NCAG-A, NCAG, and NCAG-S, respectively, and pore sizes of 36–38 nm called mesopores. NCAG presented the highest capacity to trap reactive oxygen species (106.8 mg catechin Eq./g., 86.5 % OH• trapped, respectively). All samples showed the capacity to delay the oxidation of a protein system in a dose-dependent manner, with IC<sub>50</sub> values of 70 mg/L (NCAG), 176.3 mg/L (NCAG-A), and 255.6 mg/L (NCAG-S). <em>In vitro</em> digestion showed that NCAG-S was more efficient in delivering anthocyanins under gastric conditions (bioaccessibility of 59.3 %), and NCAG under duodenal conditions (bioaccessibility of 88.2 %). The differences found in samples for the different functional assays can be explained by the various types of interactions generated between the antioxidant molecules and aerogels, in the various media where the analyses are carried out. The results indicate nanocellulose-based aerogels, synthesized from lignocellulosic residues of <em>H. macrocarpa</em> seeds, proved to be porous matrices capable of carrying bioactive substances, and presented interesting properties for the delivery and conservation of antioxidant molecules such as anthocyanins and other polyphenols, achieving an <em>in vitro</em> protective effect against the oxidation of biomolecules.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"6 ","pages":"Article 100187"},"PeriodicalIF":4.6,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Citri Reticulatae Pericarpium ‘Chachi’ (Citrus reticulata ‘Chachi’) is a traditional Chinese medicine with dual medicinal and dietary uses. As its main component, pectin exhibited various biological activities. However, research and development on it, especially pectin oligosaccharides, remain limited. In this study, ‘Chachi’ pectin oligosaccharides (CPOS) was extracted and prepared for the first time, resulting in the purification of two oligosaccharide components, CPOS3 and CPOS4. Structural characterization of the predominant oligosaccharide, CPOS3, revealed it to be composed of a backbone α-D-GalpA-(1→2)-α-L-Rhap-(1→4)-α-D-3-OAc-GalpA-(1→4)-α-D-GalpA-(1→4)-α-D-GalpA and a side chain [→5)-α-L-Araf-(1→]4 attached at the C-4 of Rhap. Subsequent experiments have shown that CPOS3 can significantly promote the proliferation and migration of human immortalized keratinocyte cell. Further investigation revealed that it facilitated cell proliferation by stimulating DNA synthesis in the S phase of the cell cycle. Additionally, CPOS3 exhibited good thermal stability and rheological characteristics. Based on the good wound healing activity and favorable physicochemical properties of CPOS3, its prospects in the fields of wound dressings and tissue repair are promising.
{"title":"Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration","authors":"Zhongcan Peng , Shurong Tian , Depo Yang , Longping Zhu , Jianing Zhang , Wenfeng Li , Guodong Zheng , Zhimin Zhao","doi":"10.1016/j.fhfh.2024.100185","DOIUrl":"10.1016/j.fhfh.2024.100185","url":null,"abstract":"<div><div>Citri Reticulatae Pericarpium ‘Chachi’ (<em>Citrus reticulata</em> ‘Chachi’) is a traditional Chinese medicine with dual medicinal and dietary uses. As its main component, pectin exhibited various biological activities. However, research and development on it, especially pectin oligosaccharides, remain limited. In this study, ‘Chachi’ pectin oligosaccharides (CPOS) was extracted and prepared for the first time, resulting in the purification of two oligosaccharide components, CPOS3 and CPOS4. Structural characterization of the predominant oligosaccharide, CPOS3, revealed it to be composed of a backbone α-D-Gal<em>p</em>A-(1→2)-α-L-Rha<em>p</em>-(1→4)-α-D-3-OAc-Gal<em>p</em>A-(1→4)-α-D-Gal<em>p</em>A-(1→4)-α-D-Gal<em>p</em>A and a side chain [→5)-α-L-Ara<em>f</em>-(1→]<sub>4</sub> attached at the C-4 of Rha<em>p</em>. Subsequent experiments have shown that CPOS3 can significantly promote the proliferation and migration of human immortalized keratinocyte cell. Further investigation revealed that it facilitated cell proliferation by stimulating DNA synthesis in the S phase of the cell cycle. Additionally, CPOS3 exhibited good thermal stability and rheological characteristics. Based on the good wound healing activity and favorable physicochemical properties of CPOS3, its prospects in the fields of wound dressings and tissue repair are promising.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"6 ","pages":"Article 100185"},"PeriodicalIF":4.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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