Food Science and Human Wellness最新文献_第8页

Molecular mechanisms of aflatoxin neurotoxicity and potential neuroprotective agents 黄曲霉毒素神经毒性的分子机制和潜在的神经保护剂

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250201

Aflatoxins (AFTs) represent one of the most notorious classes of deadly mycotoxins produced by certain fungi that are found on agricultural crops. Aflatoxins are highly toxic to mammals and are known to cause a series of detrimental effects, including neuro-, hepato-, nephron-, and immuno-toxicity. In this original review we summarize the mechanisms of aflatoxin-induced neurotoxicity and the clinical potential of novel neuroprotective agents. Aflatoxin B1 (AFB1) is the most toxic congener among the 21 identified AFTs. Recent studies have shown that food borne exposure to AFB1 and/or its metabolites often leads to fatal neurotoxicity in animals and humans. Animal studies indicated that AFB1 exposure could induce abnormal behavioral changes, including anxiety, lethargy disorders, depression-like behavior, cognitive, learning and memory defects, and decreased feeding behavior. Mechanistically, AFB1 exposure has been associated with lipid peroxidation, ablation of non-enzymatic and enzymatic antioxidant defense systems and decreased neurotransmitter levels. AFB1 exposure has also been shown to induce DNA damage, apoptosis, pyroptosis, and mitochondrial dysfunction in the brain tissue. Several signaling pathways, including gasdermin D, toll like receptor 2 (TLR2), TLR4, Akt, NF-κB, ERK/MAPK, protein kinase C (PKC), and mitochondrial apoptotic pathways have been shown to participate in AFB1-induced neuronal or astrocyte cell death. Targeting these pathways by small molecules (e.g., quercetin, curcumin, and gallic acid, and dimethyl fumarate), Chinese herbal extracts (e.g., Artichoke leaf extract, Chelidonium majus ethanolic extract, pumpkin extract, and Crocus sativus L. tea), and probiotic supplements could effectively improve AFB1-induced neurobehavioral abnormalities and neurotoxicity. To date, the precise molecular mechanisms of AFB1-induced neurotoxicity and potential neuroprotective agents remain unclear. In the present review, the clinical manifestations, molecular mechanisms, and potential neuroprotective agents of AFB1-induced neurotoxicity are summarized in the broadest overview. It is most hopeful that this broad reaching review provides valuable insights and stimulates broader discussion to develop the effective neuroprotective agents against aflatoxins.

黄曲霉毒素（AFTs）是最臭名昭著的一类致命霉菌毒素，由农作物上的某些真菌产生。黄曲霉毒素对哺乳动物有剧毒，已知会造成一系列有害影响，包括神经、肝、肾和免疫毒性。在这篇原创性综述中，我们总结了黄曲霉毒素诱发神经毒性的机制以及新型神经保护剂的临床潜力。黄曲霉毒素 B1（AFB1）是已发现的 21 种黄曲霉毒素中毒性最强的同系物。最近的研究表明，动物和人类通过食物摄入黄曲霉毒素 B1 和/或其代谢物往往会导致致命的神经中毒。动物研究表明，摄入 AFB1 可诱发异常行为变化，包括焦虑、嗜睡障碍、抑郁样行为、认知、学习和记忆缺陷以及摄食行为减少。从机理上讲，暴露于 AFB1 与脂质过氧化、非酶和酶抗氧化防御系统的消减以及神经递质水平的下降有关。研究还表明，暴露于 AFB1 会诱发脑组织的 DNA 损伤、细胞凋亡、热凋亡和线粒体功能障碍。一些信号通路，包括 gasdermin D、toll like receptor 2 (TLR2)、TLR4、Akt、NF-κB、ERK/MAPK、蛋白激酶 C (PKC) 和线粒体凋亡通路已被证明参与了 AFB1 诱导的神经元或星形胶质细胞死亡。通过小分子药物（如槲皮素、姜黄素、没食子酸和富马酸二甲酯）、中草药提取物（如朝鲜蓟叶提取物、车前子乙醇提取物、南瓜提取物和鳄梨茶）和益生菌补充剂来靶向这些通路，可有效改善 AFB1 诱导的神经行为异常和神经毒性。迄今为止，AFB1诱导神经毒性的确切分子机制和潜在的神经保护剂仍不清楚。本综述对 AFB1 诱导的神经毒性的临床表现、分子机制和潜在的神经保护剂进行了最广泛的概述。希望这篇内容广泛的综述能提供有价值的见解，并引发更广泛的讨论，从而开发出有效的黄曲霉毒素神经保护剂。

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引用次数: 0

Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo 何首乌中的薯蓣皂苷可诱导石川人子宫内膜癌细胞和体内细胞凋亡和自噬

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250209

With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma (EC), we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization. The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth. The high-throughput sequencing data indicated that dioscin not only promoted apoptosis, including decrease of poly ADP-ribose polymerase (PARP) and B-cell lymphoma-2 (Bcl-2) and increase of c-PARP and Bcl-2-associcated agonist of cell death (Bad), but also induced autophagy, including increase of autophagic lysosomes and LC3II/LC3Ⅰ ratio. Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway. Besides, the dioscin-regulated p53 pathway was mainly involved in autophagy induction. Furthermore, inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis. Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

为了理解地奥司钦对子宫内膜癌（EC）的精确调控机制，我们首先从西伯利亚何首乌中提取了成分，然后进行了鉴定和结构表征。根据对石川细胞增殖和肿瘤生长的抑制作用，初步确定了薯蓣皂苷的抗子宫内膜癌活性。高通量测序数据表明，薯蓣皂苷不仅能促进细胞凋亡，包括减少多聚 ADP 核糖聚合酶（PARP）和 B 细胞淋巴瘤-2（Bcl-2），增加 c-PARP 和 Bcl-2 细胞死亡激动剂（Bad），还能诱导自噬，包括增加自噬溶酶体和 LC3II/LC3Ⅰ 比值。机理研究表明，地奥辛通过抑制PI3K/AKT/mTOR信号通路诱导自噬和细胞凋亡。此外，地奥司钦调控的p53通路主要参与自噬诱导。此外，石川细胞自噬的抑制与 dioscin 诱导的细胞凋亡有关。我们的数据表明，薯蓣皂苷在开发欧共体功能食品和相关医疗应用方面具有巨大潜力。

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引用次数: 0

Lactobacillus from fermented bamboo shoots prevents inflammation in DSS-induced colitis mice via modulating gut microbiome and serum metabolites 发酵竹笋中的乳酸菌通过调节肠道微生物组和血清代谢物预防 DSS 诱导的结肠炎小鼠的炎症

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250229

Fermented bamboo shoots (FBS) is a region-specific food widely consumed in Southwestern China, with Lactobacillus as the predominant fermenting bacteria. However, the probiotic potential of Lactobacillus derived from FBS reminds largely unexplored, especially for diseases with a low prevalence in areas consuming FBS, namely, inflammatory bowel disease. In this study, Lactiplantibacillus pentosus YQ001 and Lentilactobacillus senioris YQ005 were screening by in vitro probiotic tests to further investigate the probiotic-like bioactivity in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mouse. They exhibited more positive probiotic effects than Lactobacillus rhamnosus GG in preventing intestinal inflammatory response. The results revealed that both strains improved the abundance of deficient intestinal microbiota in UC mice, including Muribaculaceae and Akkermansia. In the serum metabolome, they modulated the DSS-disturbed levels of metabolites, with significant increment of cinnamic acid. Meanwhile, they reduced the expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) inflammatory factors and increased zonula occludens-1 (ZO-1), Occludin, and cathelicidin-related antimicrobial peptide (CRAMP) in the colon. Consequently, these results demonstrated that Lactobacillus spp. isolates derived from FBS showed promising probiotic activity based on the gut microbiome homeostasis modulation, anti-inflammation and intestinal barrier protection in UC mice.

发酵竹笋（FBS）是中国西南地区广泛食用的一种地方特色食品，其主要发酵菌是乳酸杆菌。然而，从发酵竹笋中提取的乳酸杆菌的益生潜力尚未得到充分开发，尤其是对食用发酵竹笋的地区发病率较低的疾病（即炎症性肠病）的益生潜力。本研究通过体外益生菌试验筛选了戊糖乳杆菌（Lactiplantibacillus pentosus YQ001）和高级扁豆乳杆菌（Lentilactobacillus senioris YQ005），以进一步研究它们在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）小鼠中的益生菌样生物活性。与鼠李糖乳杆菌 GG 相比，它们在预防肠道炎症反应方面表现出更积极的益生作用。研究结果表明，这两种菌株都能改善 UC 小鼠肠道微生物区系的丰度，包括 Muribaculaceae 和 Akkermansia。在血清代谢组中，这两种菌株调节了被DSS干扰的代谢物水平，其中肉桂酸显著增加。同时，它们降低了结肠中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）等炎症因子的表达水平，并增加了结肠中Zonula occludens-1（ZO-1）、Occludin和cathelicidin相关抗菌肽（CRAMP）的表达水平。因此，这些结果表明，基于对 UC 小鼠肠道微生物组平衡的调节、抗炎和肠道屏障保护，从 FBS 中分离的乳酸杆菌具有良好的益生菌活性。

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引用次数: 0

Longitudinal investigation of mineral composition in human milk and its correlation with infant anthropometric outcomes among Tibetan mother-infant dyads during the first 6 months postpartum 产后 6 个月藏族母婴二人组母乳中矿物质成分及其与婴儿人体测量结果相关性的纵向调查

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250231

Nutrients in human milk, including minerals, relate growth and development of breast-fed infants. Tibetan mother-infant dyads possess unique characteristics on early nutrition due to their featured long-lasting life-style. This study longitudinally investigated the relationship between the mineral composition in human milk and the Z-scores of infants among Tibetan mother-infant dyads during their first 6 months postpartum through a prospective cohort study. The results show that the minerals of Na, Mg, K, Ca, Cu, Zn, and Se were of higher levels in colostrum than other lactation stages. Several minerals were below the recommended values for infants according to Chinese dietary guidelines. Besides, a large proportion of infant Z-scores were below −2 as lactation period continued. Multivariate statistical analysis revealed that classifications and correlations in varying degrees were observed between minerals in human milk and infant Z-scores. These findings will be advantageous for research upon Chinese early nutrition and progress of tailor-made infant formula.

母乳中的营养物质（包括矿物质）与母乳喂养婴儿的生长发育息息相关。藏族母婴由于其独特的长期生活方式，在早期营养方面具有独特的特点。本研究通过前瞻性队列研究，纵向调查了藏族母婴产后 6 个月内母乳中矿物质成分与婴儿 Z 评分之间的关系。结果显示，初乳中 Na、Mg、K、Ca、Cu、Zn 和 Se 等矿物质的含量高于其他哺乳阶段。有几种矿物质低于中国膳食指南对婴儿的推荐值。此外，随着哺乳期的延长，大部分婴儿的 Z 值低于-2。多变量统计分析显示，母乳中的矿物质与婴儿 Z 值之间存在不同程度的分类和相关性。这些研究结果将有助于中国早期营养的研究和量身定制婴儿配方奶粉的进展。

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引用次数: 0

DIA-based quantitative proteomic analysis on porcine meat quality at different chilling rates 基于 DIA 的不同冷冻速率下猪肉质量的定量蛋白质组分析

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250206

The objective of this study was to evaluate the effects of chilling rate on porcine meat quality from the perspective of proteome using data independent acquisition (DIA)-based quantitative proteomic strategy. M. longissimus thoracis et lumborum (n = 9) was assigned randomly to the control group (3.72 °C/h), very fast chilling-I group (VFC-I, 9.31 °C/h) and VFC-II group (14.43 °C/h). The DIA was used to analyze the difference in proteins under different chilling rates. Results showed that tenderness was improved significantly in meat at the chilling rate of 14.43 °C/h. Seventy-nine differential abundant proteins (fold change > 1.5, P < 0.05), including 46 up-regulated and 33 down-regulated proteins, were identified and mainly involved in carbon metabolism, pyruvate metabolism and proteasome pathways. These pathways indicated that VFC delayed cell metabolism and glycolysis by down-regulating the expression of metabolic enzymes. The tenderness was improved by up-regulating the expression of proteasome and m-calpain.

本研究旨在采用基于数据独立采集（DIA）的定量蛋白质组学策略，从蛋白质组的角度评估冷冻速度对猪肉品质的影响。将长胸肌和腰肌（n = 9）随机分配到对照组（3.72 °C/h）、快速冷却-I 组（VFC-I，9.31 °C/h）和 VFC-II 组（14.43 °C/h）。使用 DIA 分析了不同冷却速度下蛋白质的差异。结果表明，冷冻速率为 14.43 °C/h 时，肉的嫩度明显提高。共鉴定出79个差异丰度蛋白（折合变化率为1.5，P为0.05），包括46个上调蛋白和33个下调蛋白，主要涉及碳代谢、丙酮酸代谢和蛋白酶体途径。这些途径表明，VFC 通过下调代谢酶的表达，延迟了细胞代谢和糖酵解。通过上调蛋白酶体和 m-calpain 的表达，嫩度得到改善。

引用次数: 0

Dietary polyphenols reduced the allergenicity of β-lactoglobulin via non-covalent interactions: a study on the structure-allergenicity relationship 膳食多酚通过非共价相互作用降低了β-乳球蛋白的致敏性：关于结构-致敏性关系的研究

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250210

Studies showed that complexation of polyphenols with milk allergens reduced their immunogenic potential. However, the relationship between structures of polyphenols and their hypoallergenic effects on milk allergens in association with physiological and conformational changes of the complexes remain unclear. In this study, polyphenols from eight botanical sources were extracted to prepare non-covalent complexes with β-lactoglobulin (β-LG), a major allergen in milk. The dominant phenolic compounds bound to β-LG with a diminished allergenicity were identified to investigate their respective role on the structural and allergenic properties of β-LG. Extracts from Vaccinium fruits and black soybeans were found to have great inhibitory effects on the IgE- and IgG-binding abilities of β-LG. Among the fourteen structure-related phenolic compounds, flavonoids and tannins with larger MWs and multi-hydroxyl substituents, notably rutin, EGCG, and ellagitannins were more potent to elicit changes on the conformational structures of β-LG to decrease the allergenicity of complexed β-LG. Correlation analysis further demonstrated that a destabilized secondary structure and protein depolymerization caused by polyphenol-binding were closely related to the allergenicity property of formed complexes. This study provides insights into the understanding of structure-allergenicity relationship of β-LG-polyphenol interactions and would benefit the development of polyphenol-fortified matrices with hypoallergenic potential.

研究表明，多酚与牛奶过敏原的复合物可降低其免疫原性。然而，多酚的结构及其对牛奶过敏原的低过敏效应与复合物的生理和构象变化之间的关系仍不清楚。本研究从八种植物中提取多酚，制备与牛奶中主要过敏原 β-乳球蛋白（β-LG）的非共价复合物。研究人员确定了与β-LG结合后过敏性降低的主要酚类化合物，以研究它们各自对β-LG的结构和过敏特性所起的作用。研究发现，从越橘果实和黑大豆中提取的提取物对 β-LG 的 IgE 和 IgG 结合能力有很大的抑制作用。在 14 种结构相关的酚类化合物中，具有较大分子量和多羟基取代基的黄酮类和单宁类化合物，尤其是芦丁、EGCG 和鞣花丹宁类化合物，能更有效地引起 β-LG 构象结构的变化，从而降低与 β-LG 复合物的致敏性。相关分析进一步表明，多酚结合导致的二级结构不稳定和蛋白质解聚与形成的复合物的致敏性密切相关。这项研究为了解β-LG-多酚相互作用的结构-致敏性关系提供了深入的见解，有利于开发具有低致敏潜力的多酚强化基质。

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引用次数: 0

Application and prospects of proteomic technology in inflammation: a review 蛋白质组技术在炎症中的应用与前景：综述

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250248

Senye Wang , Yanhai Chu , Jiajia Yuan , Yiqi Li , Zhenhua Liu , Xiaoyu Chen , Wenyi Kang

Proteomics is a new technology that has been widely applied in the field of life and health science. It effectively addresses issues related to the impact of dietary structure on organs, tissues, and cells, as well as the changes in proteins in various organs, tissues, and cells under disease conditions. The differential proteins identified through proteomics can serve as disease biomarkers and target proteins affecting health and can be used for disease diagnosis and health regulation. In this paper, the application of proteomics in the field of inflammation in recent years was summarized, especially in the therapeutic target and mechanism of action, which opens up a new way for more effective prevention, diagnosis, and treatment of inflammation, and provides medical protection for human life and health.

蛋白质组学是生命与健康科学领域广泛应用的一项新技术。它能有效解决饮食结构对器官、组织和细胞的影响，以及疾病条件下各种器官、组织和细胞中蛋白质的变化等相关问题。通过蛋白质组学发现的差异蛋白质可作为疾病生物标志物和影响健康的靶蛋白，可用于疾病诊断和健康调控。本文总结了近年来蛋白质组学在炎症领域的应用，尤其是在治疗靶点和作用机制方面的应用，为更有效地预防、诊断和治疗炎症开辟了新途径，为人类生命健康提供医学保障。

引用次数: 0

Effect of exogenous free Nε-(carboxymethyl)lysine on diabetes-associated cognitive dysfunction: neuroinflammation, and metabolic disorders 外源性游离 Nε-(羧甲基)赖氨酸对糖尿病相关认知功能障碍、神经炎症和代谢紊乱的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250240

Diabetes-associated cognitive dysfunction has already been attracted considerable attention. Advanced glycation end products (AGEs) from daily diets are thought to be a vital contributor to the development of this diseases. However, the effect of one of the best-characterized exogenous AGEs N^ε-(carboxymethyl)lysine (CML) on cognitive function is not fully reported. In the present study, diabetical Goto-Kakizaki (GK) rats were treated with free CML for 8-weeks. It was found that oral consumption of exogenous CML significantly aggravated diabetes-associated cognitive dysfunction in behavioral test. In details, exogenous CML increased levels of oxidative stress, promoted the activation of glial cells in the brain, up-regulated the release of inflammatory cytokines interleukin-6, inhibited the protein expression of the brain-derived neurotrophic factor and thus led to neuroinflammation. Furthermore, exogenous CML promoted the amyloidogenesis in the brain of GK rats, and inhibited the expression of GLUT4. Additionally, several tricarboxylic acid cycle and glutamate-glutamine/γ-aminobutyric acid cycle intermediates including pyruvate, succinic acid, glutamine, glutamate were significantly changed in brain of GK rats treated with exogenous free CML. In conclusion, exogenous free CML is a potentially noxious compounds led to aggravated diabetes-associated cognitive dysfunction which could be possibly explained by its effects on neuroinflammation, energy and neurotransmitter amino acid homeostasis.

与糖尿病相关的认知功能障碍已经引起了广泛关注。日常饮食中的高级糖化终产物（AGEs）被认为是导致这种疾病发生的重要因素。然而，关于外源性 AGEs Nε-(羧甲基)赖氨酸（CML）对认知功能的影响，目前还没有充分的报道。在本研究中，糖尿病后藤柿崎（GK）大鼠接受了为期 8 周的游离 CML 治疗。结果发现，在行为测试中，口服外源性 CML 会明显加重糖尿病相关的认知功能障碍。具体而言，外源性 CML 增加了氧化应激水平，促进了脑胶质细胞的活化，上调了炎症细胞因子白细胞介素-6 的释放，抑制了脑源性神经营养因子的蛋白表达，从而导致神经炎症。此外，外源性 CML 会促进 GK 大鼠脑内淀粉样蛋白的生成，并抑制 GLUT4 的表达。此外，用外源性游离 CML 处理的 GK 大鼠脑中的一些三羧酸循环和谷氨酸-谷氨酰胺/γ-氨基丁酸循环中间产物（包括丙酮酸、琥珀酸、谷氨酰胺、谷氨酸）发生了显著变化。总之，外源性游离 CML 是一种可能导致糖尿病相关认知功能障碍加重的有毒化合物，这可能是由于它对神经炎症、能量和神经递质氨基酸平衡的影响。

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引用次数: 0

Anti-biofilm and anti-virulence potential of cell free supernatant of Akkermansia muciniphila against Salmonella Akkermansia muciniphila 细胞游离上清液对沙门氏菌的抗生物膜和抗病毒潜力

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250215

Akkermansia muciniphila is one of the commensals residing within the mammalian gut and co-evolving with the host. Numerous studies have demonstrated the benefits of A. muciniphila in ameliorating metabolic disorders, while little is known about the antimicrobial potential of A. muciniphila against pathogens. Here, we examined the antimicrobial and anti-virulence properties of cell free supernatant (CFS) of A. muciniphila against Salmonella Typhimurium. CFS retarded bacterial growth and inhibited the motility of S. Typhimurium SL1344 and S. Typhimurium 14028. CFS dose-dependently reduced cell hydrophobicity and auto-aggregation of both strains. Also, CFS from A. muciniphila significantly attenuated biofilm formation. Compared with untreated bacteria, CFS-treated bacteria significantly decreased adhesion and invasion to Caco-2 cells, and reduced intracellular survival in macrophages. CFS maintained antimicrobial properties after treatment with high temperatures and various proteases, while it lost its antimicrobial activity after pH neutralization. Gas chromatography-mass spectrometry (GC-MS) confirmed that A. muciniphila produced a certain amount of acetate and propionate, and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) identified other organic acids and metabolites in CFS. In summary, CFS from A. muciniphila exhibited anti-biofilm and anti-virulence properties against Salmonella and could be potentially utilized in the food industry for controlling Salmonella contamination and reducing infection.

Akkermansia muciniphila 是哺乳动物肠道内的共生菌之一，与宿主共同进化。大量研究表明，粘多糖对改善代谢紊乱有好处，但对粘多糖针对病原体的抗菌潜力却知之甚少。在这里，我们研究了粘菌素细胞游离上清液（CFS）对鼠伤寒沙门氏菌的抗菌和抗病毒特性。CFS 可延缓细菌生长并抑制鼠伤寒沙门氏菌 SL1344 和鼠伤寒沙门氏菌 14028 的运动。CFS剂量依赖性地降低了这两种菌株的细胞疏水性和自动聚集性。此外，来自 A. muciniphila 的 CFS 能显著减少生物膜的形成。与未经处理的细菌相比，经 CFS 处理的细菌能明显减少对 Caco-2 细胞的粘附和侵袭，并降低巨噬细胞的细胞内存活率。CFS 在经过高温和各种蛋白酶处理后仍能保持抗菌特性，而在 pH 中和后则失去了抗菌活性。气相色谱-质谱法（GC-MS）证实，A. muciniphila 产生了一定量的乙酸盐和丙酸盐，超高效液相色谱-质谱法（UHPLC-MS）鉴定出了 CFS 中的其他有机酸和代谢物。总之，粘液虹彩菌的 CFS 具有抗沙门氏菌生物膜和抗沙门氏菌病毒的特性，可用于食品工业控制沙门氏菌污染和减少感染。

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引用次数: 0

Mechanisms and active substances of targeting lipid peroxidation in ferroptosis regulation 以脂质过氧化为目标调控铁变态反应的机制和活性物质

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250204

Ferroptosis is a novel form of cell death driven by iron-dependent lipid peroxidation and it is implicated in various diseases, such as liver disease, acute kidney injury, cardiovascular disease, neurodegenerative disease and cancer. Lipid-based reactive oxygen species (ROS), particularly lipid hydroperoxides in the cellular membrane can lead to membrane disruption and cell death mediated by ferroptosis. There are three necessary stages involving in the process of lipid peroxidation regulation in ferroptosis, including the synthesis of membrane phospholipids, initiation of lipid peroxidation and clearance of lipid peroxides. In this review, we summarized the molecular modulation mechanisms of lipid peroxidation in ferroptosis from the above three stages, as well as various ferroptosis modulators targeting lipid peroxidation, including commonly used products, natural bioactive compounds and selenocompounds. Collectively, these findings suggest the vital role of lipid peroxidation in ferroptosis, and targeting lipid peroxidation in ferroptosis is potential to treat ferroptosis-associated diseases.

铁变态反应是由铁依赖性脂质过氧化驱动的一种新型细胞死亡形式，与肝病、急性肾损伤、心血管疾病、神经退行性疾病和癌症等多种疾病有关。以脂质为基础的活性氧（ROS），特别是细胞膜上的脂质氢过氧化物，可导致膜破坏，并在铁氧化作用的介导下导致细胞死亡。铁变态反应中的脂质过氧化调节过程涉及三个必要阶段，包括膜磷脂的合成、脂质过氧化的启动和脂质过氧化物的清除。在这篇综述中，我们从上述三个阶段总结了铁变态反应中脂质过氧化的分子调节机制，以及针对脂质过氧化的各种铁变态反应调节剂，包括常用产品、天然生物活性化合物和硒化合物。总之，这些研究结果表明脂质过氧化在铁变态反应中的重要作用，而针对铁变态反应中的脂质过氧化具有治疗铁变态反应相关疾病的潜力。

引用次数: 0