Food Science and Human Wellness最新文献_第9页

Structural characteristics of phenylboronic acid-modified astaxanthin ester and its effect on DSS-induced ulcerative colitis by blocking reactive oxygen species and maintaining intestinal homeostasis 苯硼酸改性虾青素酯的结构特征及其通过阻断活性氧和维持肠道稳态对 DSS 诱导的溃疡性结肠炎的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250223

A novel and reactive oxygen species (ROS) responsive astaxanthin phenylboronic acid derivative (AstaD-PBA) was constructed by grafting phenylboronic acid (PBA) onto astaxanthin succinate diester (AstaD), and its chemical structure and physicochemical property were identified. AstaD-PBA could effectively improve the ROS quenching ability in the lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation model. Then, the bioactivity of AstaD-PBA was studied by 4 zebrafish ROS-responsive inflammatory models induced by LPS, copper (Cu²⁺), high-fat diet, and dextran sodium sulfate (DSS). The results suggest that AstaD-PBA might have high biosafety and the best effect on ulcerative colitis (UC) induced by DSS. Furtherly, AstaD-PBA significantly alleviated and treated weight loss and colonic shrinkage, inhibited inflammatory cytokines, and maintained microbiota homeostasis to improve UC in C57BL/6J mice. Alistipes and Oscillibacter were expected to be considered UC marker flora according to the Metastats analysis and Pearson correlation Mantel test (P < 0.01) of 16S rRNA gene sequencing data. In conclusion, AstaD-PBA has been promised to be a functional compound to improve UC and maintain intestinal microbiota homeostasis.

通过将苯硼酸（PBA）接枝到虾青素琥珀酸二酯（AstaD）上，构建了一种新型的活性氧（ROS）反应性虾青素苯硼酸衍生物（AstaD-PBA），并鉴定了其化学结构和理化性质。在脂多糖（LPS）诱导的RAW264.7细胞炎症模型中，AstaD-PBA能有效提高ROS淬灭能力。随后，通过LPS、铜（Cu2+）、高脂饮食和右旋糖酐硫酸钠（DSS）诱导的4种斑马鱼ROS反应性炎症模型研究了AstaD-PBA的生物活性。结果表明，AstaD-PBA 可能具有较高的生物安全性，对 DSS 诱导的溃疡性结肠炎（UC）具有最佳效果。此外，AstaD-PBA 还能显著缓解和治疗 C57BL/6J 小鼠的体重减轻和结肠萎缩，抑制炎症细胞因子，维持微生物群平衡，从而改善 UC。根据 16S rRNA 基因测序数据的 Metastats 分析和 Pearson 相关性 Mantel 检验（P < 0.01），Alistipes 和 Oscillibacter 被认为是 UC 标志菌群。总之，AstaD-PBA有望成为改善UC和维持肠道微生物群平衡的功能性化合物。

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引用次数: 0

Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk 脱脂乳中的保加利亚乳杆菌生物降解有机磷农药毒死蜱的分子机制转录组分析

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250244

Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years, though the biodegradation mechanism and derived intermediate products remain unclear. This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide, chlorpyrifos, in milk by Lactobacillus delbrueckii ssp. bulgaricus using gas chromatography- tandem mass spectrometry (GC-MS/MS) combined with transcriptome analysis. After the strain was cultured for 20 h in the presence of chlorpyrifos, differential expressions of 383 genes were detected, including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase, phosphoesterase, diphosphatase, oxidoreductase, dehydratase, as well as membrane transporters. GC-MS/MS analysis revealed the changes of secondary metabolites in L. bulgaricus during milk fermentation due to chlorpyrifos stress. 6-Methylhexahydro-2H-azepin-2-one, 2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates, along with the proposed pathways, might be involved in chlorpyrifos biodegradation by L. bulgaricus.

近年来，利用益生菌对受污染食品中的有机磷农药进行生物修复日益受到关注，但其生物降解机制和衍生的中间产物仍不清楚。本研究旨在填补这一知识空白，采用气相色谱-串联质谱法（GC-MS/MS）结合转录组分析，研究了保加利亚乳杆菌对牛奶中有机磷农药毒死蜱的降解机制。在毒死蜱存在下培养该菌株 20 小时后，检测到 383 个基因有不同的表达，其中包括可能与毒死蜱降解有关的基因，如与水解酶、磷酸酯酶、二磷酸酶、氧化还原酶、脱水酶以及膜转运体有关的基因。气相色谱-质谱/质谱分析表明，在牛奶发酵过程中，由于毒死蜱胁迫，保加利亚鹅膏菌中的次生代谢物发生了变化。作为中间产物的 6-甲基六氢-2H-氮杂卓-2-酮、2,6-二羟基吡啶和 2-氨基氧基-4-甲基戊酸甲酯以及所提出的途径可能参与了保加利亚酵母菌对毒死蜱的生物降解。

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引用次数: 0

Curcumin delivery nanoparticles based on Maillard reaction of Haematococcus pluvialis protein/galactose for alleviating acute alcoholic liver damage 基于血球菌蛋白/半乳糖的马氏反应的姜黄素递送纳米颗粒，用于缓解急性酒精性肝损伤

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250211

The aim of this study is to investigate the feasibility of Maillard reaction products of Haematococcus pluvialis protein and galactose (HPP-GAL) for improving the bioactivities of curcumin (CUR) for alleviating alcoholic liver damage. CUR was embedded into HPP-GAL nanoparticles by the self-assembly of hydrogen bonding and hydrophobic interaction with the particle size around 200 nm. HPP-GAL enhanced the encapsulation efficiency and loading amount of CUR with the value of (89.21 ± 0.33)% and (0.500 ± 0.004)%, respectively. The stabilities of CUR under strong acid, salt ion stability and ultraviolet irradiation conditions were improved by the encapsulation. HPP-GAL-CUR nanoparticles exhibited excellent concentration-dependent in vitro antioxidant activities including DPPH and ABTS scavenging rates, and better protective effect on CUR against gastric acid environment as well as longer release of CUR in simulated intestinal fluid. In addition, the HPP-GAL-CUR delivery system possessed liver targeting property due to the existence of GAL, which could effectively alleviate the alcohol-induced liver damage and the inflammation indexes by inhibiting the oxidative stress. Therefore, HPP-GAL-CUR nanoparticles might be a potential candidate system for the prevention of alcoholic liver damage in the future.

本研究旨在探讨血球菌蛋白与半乳糖的马氏反应产物（HPP-GAL）改善姜黄素（CUR）生物活性以减轻酒精性肝损伤的可行性。通过氢键和疏水作用的自组装，姜黄素被嵌入 HPP-GAL 纳米粒子，粒径约为 200 nm。HPP-GAL提高了CUR的包封效率和负载量，其值分别为（89.21 ± 0.33）%和（0.500 ± 0.004）%。封装后的 CUR 在强酸、盐离子稳定性和紫外线照射条件下的稳定性都得到了改善。HPP-GAL-CUR 纳米粒子具有优异的浓度依赖性体外抗氧化活性，包括 DPPH 和 ABTS 清除率，对 CUR 在胃酸环境中具有更好的保护作用，在模拟肠液中的释放时间更长。此外，由于 GAL 的存在，HPP-GAL-CUR 给药系统具有肝脏靶向性，可通过抑制氧化应激有效缓解酒精引起的肝损伤和炎症指标。因此，HPP-GAL-CUR纳米颗粒可能是未来预防酒精性肝损伤的潜在候选系统。

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引用次数: 0

Antiglycating effects of citrus flavonoids and associated mechanisms 柑橘类黄酮的抗糖化作用及相关机制

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250247

Yunli Xiao , Junfeng Shen , Jianfeng Zhan , Limin Guo , Chi-Tang Ho , Shiming Li

Glycation of proteins and DNA forms advanced glycation end products (AGEs) causing cell and tissue dysfunction and subsequent various chronic diseases, in particular, metabolic and age-related diseases. Targeted AGE inhibition includes scavengers of reactive carbonyl species (RCS) such as methylglyoxal (MG), glyoxalase-1 enhancers, Nrf2/ARE pathway activators, AGE/RAGE formation inhibitors and other antiglycatng agents. Citrus flavonoids have demonstrated antioxidant and anti-inflammatory effects and are also found to be effective antiglycating agents. Herein, we reviewed the up to date progress of the antiglycation effects of citrus flavonoids and associated mechanisms. Major citrus flavonoids, hesperedin and its aglycone, hesperetin, inhibited glycation by scavenging MG forming mono- or di-flavonoid adducts with MG, enhanced the activity of glyoxase-1, activated Akt/Nrf2 signal pathway while inhibiting AGE/RAGE/NF-κB pathway, reduced the formation of N^ε-(carboxylmethyl)lysine (CML) and pentosidine, inhibited aldol reductase activity and decreased the levels of fructosamine. The antiglycating activity and mechanisms of other flavonoids was also summarized in this review. In conclusion, citrus flavonoids possess effective antiglycating activity via different mechanisms, yet there are many challenging questions remaining to be studied in the near future such as in vivo testing and human study of citrus flavonoids for efficacy, effectiveness and adverse effects of citrus flavonoids as a functional food in managing high levels of AGEs and controlling AGE-induced chronic diseases, diabetic complications in particular.

蛋白质和 DNA 的糖化会形成高级糖化终产物（AGEs），导致细胞和组织功能障碍，进而引发各种慢性疾病，尤其是代谢性疾病和与年龄有关的疾病。有针对性的 AGE 抑制剂包括甲基乙二醛（MG）等活性羰基（RCS）清除剂、乙醛缩醛酶-1 增强剂、Nrf2/ARE 途径激活剂、AGE/RAGE 形成抑制剂和其他抗糖化剂。柑橘类黄酮具有抗氧化和抗炎作用，也是有效的抗糖化剂。在此，我们回顾了柑橘类黄酮抗糖化作用及相关机制的最新进展。主要的柑橘类黄酮、橙皮素及其苷元橙皮素通过清除与 MG 形成单或双黄酮加合物的 MG 来抑制糖化，增强糖化酶-1 的活性、激活 Akt/Nrf2 信号通路，同时抑制 AGE/RAGE/NF-κB 通路，减少 Nε-（羧甲基）赖氨酸（CML）和喷托糖苷的形成，抑制醛醇还原酶的活性，降低果糖胺的水平。本综述还总结了其他黄酮类化合物的抗糖化活性和机制。总之，柑橘类黄酮通过不同的机制具有有效的抗糖化活性，但在不久的将来仍有许多具有挑战性的问题有待研究，如柑橘类黄酮的体内试验和人体研究，以了解柑橘类黄酮作为功能食品在控制高浓度 AGEs 和控制 AGE 引起的慢性疾病（尤其是糖尿病并发症）方面的功效、有效性和不良影响。

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引用次数: 0

Fufang E’jiao Jiang’s effect on immunity, hematopoiesis, and angiogenesis via a systematic “compound-effect-target” analysis 通过系统的 "复合效应-靶标 "分析，了解扶芳二条姜对免疫、造血和血管生成的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250228

Fufang E’jiao Jiang (FEJ) as a healthy food consisting of medicine food homology materials approved by China’s Ministry of Health has been extensively applied to replenish qi and nourish blood, and it has a positive impact on women’s health. To find out the material basis and mechanism of FEJ, a systematic “compound-effect-target” analysis including chemical composition resolution, zebrafish, network pharmacology, molecular docking, transcriptome, and bibliometric analysis was adopted. 124 chemical components including ginsenosides, and phenylethanoid glycosides in FEJ were discovered, and effects of FEJ on promoting the generation of immune cells, erythropoiesis and angiogenesis in zebrafish were exhibited. Based on network pharmacology, molecular docking and in vivo activity assay, 6 compounds including jionoside A1, isoacteoside, echinacoside, acteoside, lobetyolin, and rehmannioside D were identified as active components of FEJ. Transcriptome data showed that several pathways such as complement and coagulation cascades, ECM-receptor interaction, and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ. 19 common targets were obtained through combined analysis of network pharmacology and transcriptomics, and 5 targets of them were verified by PCR. The bibliometric analysis of these common targets revealed that FEJ was related to energy metabolism, pathway in cancer, etc., which was consistent with the results of network pharmacology and transcriptome. The studies suggested that FEJ could replenish qi and nourish blood through multi-compound and multi-targets.

馥芳娥娇姜作为中国卫生部批准的药食同源健康食品，在补气养血方面得到了广泛应用，对女性健康产生了积极影响。为探明人参皂苷的物质基础和作用机理，采用了化学成分解析、斑马鱼、网络药理学、分子对接、转录组和文献计量学等系统的 "化合物-效应-靶标 "分析方法。发现了 FEJ 中包括人参皂苷、苯乙醇苷在内的 124 种化学成分，并展示了 FEJ 促进斑马鱼免疫细胞生成、红细胞生成和血管生成的作用。基于网络药理学、分子对接和体内活性检测，6 个化合物，包括琼瑶苷 A1、异内酯苷、棘鲷苷、放线菌苷、龙脑香苷和雷曼薯蓣皂苷 D 被鉴定为 FEJ 的活性成分。转录组数据显示，补体和凝血级联、ECM-受体相互作用和 PI3K-Akt 信号通路等多个通路与 FEJ 的促血管生成作用有关。通过网络药理学和转录组学的联合分析，获得了19个共同靶点，并对其中的5个靶点进行了PCR验证。对这些共同靶点的文献计量分析表明，FEJ与能量代谢、癌症通路等有关，这与网络药理学和转录组学的结果一致。这些研究表明，FEJ 可通过多化合物和多靶点来补气养血。

{"title":"Fufang E’jiao Jiang’s effect on immunity, hematopoiesis, and angiogenesis via a systematic “compound-effect-target” analysis","authors":"","doi":"10.26599/FSHW.2022.9250228","DOIUrl":"10.26599/FSHW.2022.9250228","url":null,"abstract":"<div><div><em>Fufang E</em>’<em>jiao Jiang</em> (FEJ) as a healthy food consisting of medicine food homology materials approved by China’s Ministry of Health has been extensively applied to replenish <em>qi</em> and nourish blood, and it has a positive impact on women’s health. To find out the material basis and mechanism of FEJ, a systematic “compound-effect-target” analysis including chemical composition resolution, zebrafish, network pharmacology, molecular docking, transcriptome, and bibliometric analysis was adopted. 124 chemical components including ginsenosides, and phenylethanoid glycosides in FEJ were discovered, and effects of FEJ on promoting the generation of immune cells, erythropoiesis and angiogenesis in zebrafish were exhibited. Based on network pharmacology, molecular docking and <em>in vivo</em> activity assay, 6 compounds including jionoside A1, isoacteoside, echinacoside, acteoside, lobetyolin, and rehmannioside D were identified as active components of FEJ. Transcriptome data showed that several pathways such as complement and coagulation cascades, ECM-receptor interaction, and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ. 19 common targets were obtained through combined analysis of network pharmacology and transcriptomics, and 5 targets of them were verified by PCR. The bibliometric analysis of these common targets revealed that FEJ was related to energy metabolism, pathway in cancer, etc., which was consistent with the results of network pharmacology and transcriptome. The studies suggested that FEJ could replenish <em>qi</em> and nourish blood through multi-compound and multi-targets.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 5","pages":"Pages 2813-2832"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135372281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile SERS screening of melamine in bovine milk with 2D printed AgNPs/glass fabric filter paper as the flexible substrate 用二维印刷的 AgNPs/玻璃纤维滤纸作为柔性基底，对牛乳中的三聚氰胺进行简便的 SERS 筛选

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250208

Melamine is one of the most frequently detected adulterants in dairy products. The current study proposes a surface-enhanced Raman spectroscopy (SERS)-based analytical tool for fast and reliable screening of melamine in bovine milk. A hand-held Raman spectrometer was used in conjunction with a substrate composed of silver nanoparticles (AgNPs) that was 2D printed onto glass fiber (GF) filter paper. Under optimized conditions, a sensitive and fingerprint-like signal at 674 cm⁻¹ was obtained. The AgNPs/GF substrate exhibited high sensitivity to melamine in milk down to 1.9498 × 10⁻⁵ mg/mL, which is well below the USA and EU safety limits (2.5 × 10⁻³ mg/mL). Remarkably, the proposed technology was also highly reproducible, showing spot-to-spot and block-to-block variations below 3.3 % and 4.9 % at 674 cm⁻¹ in Raman intensity, respectively. The characteristic peak intensity and concentration of melamine showed an acceptable linear relationship (R² = 0.9909) within the range of 0.0001–1 mg/mL. Overall, the method established in this study can provide an efficient and effective method for the quantitative target screening and detection of melamine in dairy products.

三聚氰胺是乳制品中最常检测到的掺假物质之一。本研究提出了一种基于表面增强拉曼光谱（SERS）的分析工具，用于快速、可靠地筛查牛乳中的三聚氰胺。研究人员将手持式拉曼光谱仪与二维印刷在玻璃纤维（GF）滤纸上的银纳米颗粒（AgNPs）基底结合使用。在优化条件下，在 674 cm-1 处获得了灵敏的指纹样信号。AgNPs/GF 基质对牛奶中三聚氰胺的灵敏度高达 1.9498 × 10-5 mg/mL，远低于美国和欧盟的安全限值（2.5 × 10-3 mg/mL）。值得注意的是，该技术的重现性也很高，在拉曼强度674 cm-1处，点与点之间和块与块之间的变化分别低于3.3%和4.9%。在 0.0001-1 mg/mL 的范围内，特征峰强度与三聚氰胺的浓度呈可接受的线性关系（R2 = 0.9909）。总之，本研究建立的方法可为乳制品中三聚氰胺的定量目标筛选和检测提供一种高效、有效的方法。

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引用次数: 0

Impact of phytoconstituents on oral health practices: a post COVID-19 observation 植物成分对口腔卫生做法的影响：COVID-19 后的观察结果

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250197

Appropriate oral hygiene significantly reduces the possibility of oral infections. However, dental caries and periodontal diseases are major oral health issues causing chronic diseases due to poor oral health. Recently, herbal compounds have gained interest in maintaining oral health. Extracts of burdock root (Arctium), noni fruit (Morinda citrifolia), and neem leaf (Azadirachta indica) are now used as intracanal medicaments in endodontics and periodontics. Plectranthus amboinicus species and other plants produces essential oil like β-caryophyllene, p-cymene, and γ-terpinene can exhibit antibacterial activity; highlighting phytoconstituents plays a vital role in oral health. The COVID-19 pandemic highlighted the importance of hygiene and sanitization, to curb SARS-CoV-2. Oral cavity is among the gateways for virus entry into saliva. Saliva is a potential reservoir of SARS-CoV-2, and there is an increased risk of infection if there is any fissure in the mouth. This enables entry of virus into the vascular system through gingival or periodontal pocket, possibly reaching lung periphery then to lung vessels by interacting with endothelial surface receptors triggering pulmonary vasoconstriction and lung damage due to endothelial dysfunction. This review aims to draw attention to the possible route of SARS-CoV-2 infection via the oral cavity and the importance of oral hygiene against COVID-19.

适当的口腔卫生能大大降低口腔感染的可能性。然而，龋齿和牙周病是主要的口腔健康问题，由于口腔健康状况不佳而导致慢性疾病。最近，草药复合物在维护口腔健康方面引起了人们的兴趣。牛蒡根（牛蒡）、诺丽果（巴戟天）和印楝叶（Azadirachta indica）的提取物目前已被用作牙髓病学和牙周病学的根管内药物。Plectranthus amboinicus 品种和其他植物产生的精油，如 β-茶叶烯、对伞花烯和 γ-萜品烯，具有抗菌活性；突出植物成分在口腔健康中的重要作用。COVID-19 大流行凸显了卫生和消毒对遏制 SARS-CoV-2 的重要性。口腔是病毒进入唾液的门户之一。唾液是 SARS-CoV-2 的潜在贮存库，如果口腔有任何裂缝，感染的风险就会增加。这使得病毒能够通过牙龈或牙周袋进入血管系统，可能到达肺部外围，然后通过与内皮表面受体相互作用进入肺部血管，引发肺血管收缩和内皮功能障碍导致的肺损伤。本综述旨在提请人们注意经口腔感染 SARS-CoV-2 的可能途径，以及口腔卫生对 COVID-19 的重要性。

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引用次数: 0

Oligomeric procyanidins combined with Parabacteroides distasonis ameliorate high-fat diet-induced atherosclerosis by regulating lipid metabolism, inflammation reaction and bile acid metabolism in ApoE−/− mice 通过调节载脂蛋白E-/-小鼠的脂质代谢、炎症反应和胆汁酸代谢，低聚原花青素与副嗜酸杆菌结合可改善高脂饮食引起的动脉粥样硬化

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250230

Atherosclerosis (AS) is the main pathological basis of cardiovascular diseases. Hence, the prevention and treatment strategies of AS have attracted great research attention. As a potential probiotic, Pararabacteroides distasonis has a positive regulatory effect on lipid metabolism and bile acids (BAs) profile. Oligomeric procyanidins have been confirmed to be conducive to the prevention and treatment of AS, whose anti-atherosclerotic effect may be associated with the promotion of gut probiotics. However, it remains unclear whether and how oligomeric procyanidins and P. distasonis combined (PPC) treatment can effectively alleviate high-fat diet (HFD)-induced AS. In this study, PPC treatment was found to significantly decrease atherosclerotic lesion, as well as alleviate the lipid metabolism disorder, inflammation and oxidative stress injury in ApoE^−/− mice. Surprisingly, targeted metabolomics demonstrated that PPC intervention altered the BA profile in mice by regulating the ratio of secondary BAs to primary BAs, and increased fecal BAs excretion. Further, quantitative polymerase chain reaction (qPCR) analysis showed that PPC intervention facilitated reverse cholesterol transport by upregulating Srb1 expression; In addition, PPC intervention promoted BA synthesis from cholesterol in liver by upregulating Cyp7a1 expression via suppression of the farnesoid X receptor (FXR) pathway, thus exhibiting a significant serum cholesterol-lowering effect. In summary, PPC attenuated HFD-induced AS in ApoE^−/− mice, which provides new insights into the design of novel and efficient anti-atherosclerotic strategies to prevent AS based on probiotics and prebiotics.

动脉粥样硬化（AS）是心血管疾病的主要病理基础。因此，动脉粥样硬化的预防和治疗策略备受研究关注。作为一种潜在的益生菌，Pararabacteroides distasonis 对脂质代谢和胆汁酸（BAs）具有积极的调节作用。寡聚原花青素已被证实有利于强直性脊柱炎的预防和治疗，其抗动脉粥样硬化作用可能与肠道益生菌的促进作用有关。然而，低聚原花青素和P. distasonis（PPC）联合治疗是否能有效缓解高脂饮食（HFD）诱导的强直性脊柱炎，以及如何缓解，目前仍不清楚。本研究发现，PPC治疗可显著减少动脉粥样硬化病变，并缓解载脂蛋白E/-小鼠的脂质代谢紊乱、炎症和氧化应激损伤。令人惊讶的是，靶向代谢组学研究表明，PPC 通过调节次生 BA 与原生 BA 的比例，改变了小鼠体内 BA 的分布，并增加了粪便中 BA 的排泄量。此外，定量聚合酶链反应（qPCR）分析表明，PPC干预通过上调Srb1的表达，促进了胆固醇的逆向转运；PPC干预还通过抑制类雌激素X受体（FXR）通路，上调Cyp7a1的表达，促进了肝脏中胆固醇合成BA，因此具有显著的降低血清胆固醇的作用。总之，PPC可减轻HFD诱导的载脂蛋白E-/-小鼠强直性脊柱炎，这为设计基于益生菌和益生元的新型高效抗动脉粥样硬化策略以预防强直性脊柱炎提供了新的思路。

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引用次数: 0

Capsaicin alleviates the hepatic clock gene disruption and gut microbiota dysbiosis in circadian rhythm disorder mouse model 辣椒素缓解昼夜节律紊乱小鼠模型中的肝脏时钟基因紊乱和肠道微生物群失调

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250238

As the body’s internal clock, the circadian rhythm regulates the energy expenditure, appetite, and sleep. There exists a close relationship between the host circadian rhythm and gut microbiota. In this work, a circadian disorder mouse model induced by constant darkness (CD) was constructed to investigate the regulating effects of capsaicin (CAP) on disturbances of metabolism homeostasis and gut microbiota in the respect of circadian rhythm-related mechanisms. Our results indicated that CAP reduced weight gain induced by circadian rhythm disorder in mice by inhibiting fat accumulation in liver and adipose tissue. The rhythmic expressions of circadian clock genes and lipid-metabolism related genes in liver were also recovered by CAP. Microbial study using 16S rRNA sequencing revealed that CAP modulated the gut microbiota richness, diversity and composition, and restored diurnal oscillations of gut microbes at the phylum and family level. These results indicated that CAP could alleviate CD-induced hepatic clock gene disruption and gut microbiota dysbiosis in mice, providing theoretical basis for CAP to be used as a muti-functional ingredient with great health-promoting effects.

作为人体的内部时钟，昼夜节律调节着能量消耗、食欲和睡眠。宿主的昼夜节律与肠道微生物群之间存在着密切的关系。本研究构建了一个由持续黑暗（CD）诱导的昼夜节律紊乱小鼠模型，以研究辣椒素（CAP）在昼夜节律相关机制方面对代谢平衡紊乱和肠道微生物群的调节作用。结果表明，辣椒素通过抑制肝脏和脂肪组织的脂肪积累，减少了昼夜节律紊乱引起的小鼠体重增加。CAP 还能恢复肝脏中昼夜节律基因和脂质代谢相关基因的节律表达。使用 16S rRNA 测序法进行的微生物研究表明，CAP 可调节肠道微生物群的丰富度、多样性和组成，并在门和科水平上恢复肠道微生物的昼夜振荡。这些结果表明，CAP 可以缓解 CD 引起的小鼠肝脏时钟基因紊乱和肠道微生物群失调，为 CAP 作为一种具有巨大健康促进作用的多功能成分提供了理论依据。

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引用次数: 0

Structural characterization and anti-inflammatory activities of novel polysaccharides obtained from Pleurotus eryngii 从刺五加中提取的新型多糖的结构特征和抗炎活性

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250245

Natural polysaccharides named PEP-0.1-1, PEP-0-1 and PEP-0-2 from edible mushroom species Pleurotus eryngii were obtained in the present study. Results showed that molecular weights of these polysaccharides were 3235, 2041 and 23933 Da, respectively. Further, structural characterization revealed that PEP-0.1-1 had a →4-α-D-Glcp-1→ backbone and contained →4)-α-D-Glcp and →4)-β-D-Glcp reducing end groups. PEP-0-1 backbone contained →4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-1→, and the side chains contained α-D-Glcp, β-D-Manp-1→ and α-D-Glcp-3→. However, PEP-0-2 backbone consisted of →4-α-D-Glcp-1→ and →6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→ while the side chains contained α-D-Glcp and β-D-Manp-1→. Biological activity analysis was then carried out and found that all these polysaccharides could significantly suppress the relative mRNA expression of toll-like receptor 4, nitric oxide, tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in lipopolysaccharide (LPS)-induced inflammation of RAW264.7 cells, as well as the over secretion of the above cell cytokines. Moreover, Western blotting analysis revealed that all these purified fractions displayed significant inhibition effects on the expression of c-Jun N-terminal kinases protein protein induced by LPS in mitogen activated protein kinase pathway, along with the relieving on the inhibition effect of LPS on IκB-α protein expression. In summary, the information generated by the present study could provide a theoretical basis for the exploration of novel healthy food materials from edible mushroom with anti-inflammation activities.

本研究从食用菌品种刺五加（Pleurotus eryngii）中获得了名为 PEP-0.1-1、PEP-0-1 和 PEP-0-2 的天然多糖。结果表明，这些多糖的分子量分别为 3235、2041 和 23933 Da。进一步的结构表征显示，PEP-0.1-1 的骨架为 →4-α-D-Glcp-1→ ，含有 →4)-α-D-Glcp 和 →4)-β-D-Glcp 还原端基团。PEP-0-1 主干含有→4-α-D-Glcp-1→和→6-α-3-O-Me-D-Galp-1→，侧链含有α-D-Glcp、β-D-Manp-1→和α-D-Glcp-3→。然而，PEP-0-2 的骨架由 →4-α-D-Glcp-1→ 和 →6-α-3-O-Me-D-Galp-(1→6)-α-D-Galp-1→ 组成，侧链包含 α-D-Glcp 和 β-D-Manp-1→。然后进行了生物活性分析，发现所有这些多糖都能显著抑制脂多糖（LPS）诱导的 RAW264.7 细胞炎症中收费样受体 4、一氧化氮、肿瘤坏死因子-α、白细胞介素（IL）-1β 和 IL-6 的相对 mRNA 表达，以及上述细胞因子的过度分泌。此外，Western 印迹分析表明，所有这些纯化的馏分都对 LPS 在丝裂原活化蛋白激酶通路中诱导的 c-Jun N 端激酶蛋白的表达有显著的抑制作用，同时也缓解了 LPS 对 IκB-α 蛋白表达的抑制作用。总之，本研究获得的信息可为探索从食用菌中提取具有抗炎活性的新型健康食品材料提供理论依据。

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