Food Bioscience最新文献_第9页

Ultrasound enhances the probiotic properties and anti-inflammatory function of Lacticaseibacillus rhamnosus 1.0320 by releasing functional metabolites 超声通过释放功能性代谢物增强鼠李糖乳杆菌1.0320的益生菌特性和抗炎功能

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108293

Xinyu Neng , Xuefei Wu , He Zhang , Jing Zhang , Cong Xu , Jiahui Guo , Zhanmei Jiang , Juncai Hou

Postbiotics are emerging as a potential alternative strategy for regulating gut microbiota health. However, conventional inactivation methods can damage bioactive components, and the effects of ultrasonic processing parameters on postbiotic functionality remain poorly understood. This study aimed to investigate the effects of different ultrasonic parameters (400/800 W, 10–60 min) on the properties of Lacticaseibacillus rhamnosus 1.0320 and elucidate the underlying mechanism. Results indicated that complete inactivation was achieved with 400 W treatment for 60 min or 800 W treatment for 30 min. Cell damage induced by 800 W was significantly greater than that caused by 400 W (p < 0.05). Ultrasonication markedly altered bacterial surface properties, increasing hydrophobicity to 1.8 times that of viable cells, reducing self-aggregation to 11.38 %, and enhancing antioxidant capacity. The 800 W/60 min group exhibited the highest ABTS and DPPH radical scavenging rates. This group also significantly suppressed LPS-induced pro-inflammatory factors (IL-1β, TNF-α, and IL-6) while elevating anti-inflammatory factors (IL-4) (p < 0.05). Non-targeted metabolomics analysis further revealed that the destruction of cell structure triggered by the cavitation effect of ultrasound was the main reason for the functional enhancement. This study demonstrated that ultrasound is an efficient non-thermal method for producing highly active postbiotics, supporting the development of non-living anti-inflammatory microbial agents.

后生物制剂正在成为调节肠道微生物群健康的潜在替代策略。然而，传统的失活方法会破坏生物活性成分，超声波处理参数对生物后功能的影响仍然知之甚少。本研究旨在探讨不同超声参数（400/800 W, 10-60 min）对鼠李糖乳杆菌1.0320特性的影响，并探讨其作用机制。结果表明，400 W处理60 min或800 W处理30 min可达到完全失活。800 W对细胞的损伤显著大于400 W （p < 0.05）。超声能显著改变细菌的表面特性，疏水性提高到活细胞的1.8倍，自聚集性降低到11.38%，抗氧化能力增强。800 W/60 min组ABTS和DPPH自由基清除率最高。同时显著抑制lps诱导的促炎因子（IL-1β、TNF-α、IL-6），升高抗炎因子（IL-4）（p < 0.05）。非靶向代谢组学分析进一步揭示超声空化效应引发的细胞结构破坏是功能增强的主要原因。本研究表明，超声是一种高效的非热方法，用于生产高活性的后生制剂，支持非活性抗炎微生物制剂的开发。

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引用次数: 0

From traditional wet-fermented meat to modern wound -therapeutics: The transboundary journey of probiotic Lacticaseibacillus paracasei YL36 从传统的湿发酵肉到现代伤口治疗：副酪乳杆菌YL36的跨界之旅

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108288

Yiwei Zuo , Kexin Deng , Wuliang Diao , Jianda Zhou

“Suanrou”, a characteristic moist fermented meat product indigenous to Southwest China, inspiring this study based on “analogical orientation” and “shape-based nourishment” concepts of traditional Chinese medicine. This study aimed to isolate lactic acid bacteria (LAB) with broad-spectrum antibacterial activity against common infectious bacteria in chronic diabetic wounds from Suanrou to promote chronic wound healing. 347 lactic acid bacteria strains were isolated from 6 types of Suanrou samples. After many screening, including antibacterial assay, hemolysis test, antibiotic susceptibility test, and aggregation assay, Lacticaseibacillus paracasei YL36 was identified as strain with optimal comprehensive performance. Subsequent analyses of whole-genome sequencing and metabolomics were conducted, along with further validation using cellular and animal models. L. paracasei YL36 exhibited inhibitory activity against five common pathogenic bacteria in chronic wounds, including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Streptococcus haemolyticus. Genomic analysis revealed that strain harbors gene clusters responsible for synthesizing antimicrobial metabolites such as terpenoids and class II bacteriocins; its genome length was 3,029,192 bp, with a G + C content of 46.38 %. Additionally, L. paracasei YL36 possessed rich and diverse metabolic profile, with clear associations between its metabolic pathways, metabolites, and antibacterial activity. The strain displayed excellent adhesion and colonization capabilities, with no clinically relevant antibiotic resistance genes or virulence factor genes detected in its genome, and a hemolysis rate of <5 %. L. paracasei YL36 is a safe and functionally excellent probiotic candidate strain with potential application value in the field of chronic wound healing.

“酸肉”是一种中国西南地区特有的湿发酵肉制品，启发了本研究基于中医“类比取向”和“形状营养”的概念。本研究旨在从慢性糖尿病创面中分离出对常见感染性细菌具有广谱抗菌活性的乳酸菌，促进慢性糖尿病创面愈合。从6种酸味样品中分离出347株乳酸菌。经抗菌试验、溶血试验、药敏试验、聚集试验等筛选，确定副干酪乳杆菌YL36为综合性能最佳的菌株。随后进行了全基因组测序和代谢组学分析，并使用细胞和动物模型进行了进一步验证。L. paracasei YL36对慢性伤口中常见的5种致病菌，包括金黄色葡萄球菌、肺炎克雷伯菌、铜绿假单胞菌、大肠杆菌和溶血链球菌均有抑制作用。基因组分析显示，该菌株含有负责合成萜类和II类细菌素等抗菌代谢物的基因簇；基因组长度3029192 bp， G + C含量46.38%。此外，L. paracasei YL36具有丰富多样的代谢谱，其代谢途径、代谢物与抗菌活性之间存在明确的关联。该菌株表现出良好的粘附和定植能力，其基因组中未检测到临床相关的抗生素耐药基因和毒力因子基因，溶血率为5%。副casei YL36是一种安全、功能优良的益生菌候选菌株，在慢性伤口愈合领域具有潜在的应用价值。

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引用次数: 0

A comprehensive review on algal bioactive compounds with emphasize on its allergenicity reduction, health benefits and applications in food industry 对藻类生物活性化合物的研究进展进行了综述，重点介绍了藻类生物活性化合物的致敏性、健康益处及其在食品工业中的应用

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-12 DOI: 10.1016/j.fbio.2026.108285

Soubhagya Tripathy , Srutee Rout , Rahel Suchintita Das , Brijesh Kumar Tiwari , Marco Garcia-Vaquero , Prem Prakash Srivastav

Algae represent a sustainable and versatile source of bioactive compounds with immense potential in nutraceutical, pharmaceutical, and functional food applications. This review comprehensively discusses the nutritional composition and therapeutic properties of algal-derived biomolecules such as proteins, polysaccharides, lipids, vitamins, minerals, phenolics, and peptides. These compounds exhibit diverse biological activities, including antioxidant, anticancer, anti-inflammatory, antidiabetic, cardioprotective, and immune-enhancing effects. Despite their benefits, algal products may pose allergenic risks from intrinsic constituents (proteins, pigments, and polysaccharides) and extrinsic sources (marine co-inhabitants). Clinical cases and analytical studies revealing allergenic mechanisms are critically examined. Furthermore, recent advancements in non-thermal and encapsulation technologies, such as cold plasma, pulsed electric fields, high-pressure processing, and microencapsulation, are highlighted as promising strategies for mitigating allergenicity while preserving bioactivity. The application of algal bioactives in bakery, dairy, and packaging industries underscores their potential to enhance nutritional quality, functionality, and sustainability. Additionally, the review emphasizes the growing role of algae in advancing the blue economy through bioresource valorization, renewable energy production, and circular food systems. Key regulatory challenges, consumer perception, and safety concerns limiting large-scale adoption are also discussed. This review provides an integrated perspective on algae as a future-oriented bioresource, addressing their health benefits, allergenicity management, and industrial utilization while identifying knowledge gaps and future research directions toward sustainable food innovation.

藻类是一种可持续和通用的生物活性化合物来源，在营养保健、制药和功能食品应用方面具有巨大的潜力。本文综述了藻类衍生生物分子的营养成分和治疗特性，如蛋白质、多糖、脂质、维生素、矿物质、酚类物质和多肽。这些化合物具有多种生物活性，包括抗氧化、抗癌、抗炎、抗糖尿病、心脏保护和免疫增强作用。尽管它们有好处，但藻类产品可能会因其内在成分（蛋白质、色素和多糖）和外在来源（海洋共生生物）而造成过敏风险。临床病例和分析研究揭示过敏机制进行严格审查。此外，最近在非热和封装技术方面的进展，如冷等离子体、脉冲电场、高压处理和微封装，被强调为在保持生物活性的同时减轻过敏原的有希望的策略。藻类生物活性物质在烘焙、乳制品和包装行业的应用强调了它们在提高营养质量、功能和可持续性方面的潜力。此外，该综述还强调了藻类在通过生物资源增值、可再生能源生产和循环粮食系统推动蓝色经济方面日益重要的作用。还讨论了主要的监管挑战、消费者认知和限制大规模采用的安全问题。本文综述了藻类作为一种面向未来的生物资源，阐述了它们的健康益处、过敏原管理和工业利用，同时确定了可持续食品创新的知识差距和未来的研究方向。

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引用次数: 0

Evaluation of zinc protoporphyrin IX synthesis and color development in minced meat model system by diverse Staphylococcus strains: a strategy for developing nitrite-free meat products 不同葡萄球菌菌株在肉糜模型系统中合成原卟啉锌IX及显色的评价：开发无亚硝酸盐肉制品的策略

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-10 DOI: 10.1016/j.fbio.2026.108279

Yaru Yuan, Zhengyi Li, Qian Chen, Qian Liu, Baohua Kong, Chao Zhang

The application of nitrites in meat products has been associated with considerable safety concerns, as excessive nitrites can undergo conversion into carcinogenic compounds at high concentrations, thereby presenting serious health risks to consumers. To mitigate this concern, the present study utilized zinc protoporphyrin IX (ZnPP) as a safe and effective alternative to nitrites. Six strains of Staphylococcus were selected and systematically evaluated for their capacity to enhance ZnPP formation and optimize the color development of minced meat model system. All strains exhibited substantially higher intracellular than extracellular ferrochelatase activity, with Staphylococcus equorum demonstrating the most pronounced activity (P < 0.05). All the inoculated samples showed significantly elevated L∗ values and reduced b∗ values versus the controls (P < 0.05). Fluorescence spectra revealed progressively intensifying ZnPP-specific absorption peaks in Staphylococcus-inoculated groups over time. At the endpoint, S. equorum-inoculated minced meat model system achieved peak ZnPP content (893.91 ± 7.14 ng/g). Though lower than nitrite-treated samples, the S. equorum-inoculated samples exhibited significantly elevated a∗ values relative to the controls at the endpoint (P < 0.05). Collectively, S. equorum most effectively promoted ZnPP formation and color improvement in minced meat model system, positioning it as a viable coloring strategy for full/partial nitrite replacement in meat products.

在肉制品中使用亚硝酸盐引起了相当大的安全问题，因为过量的亚硝酸盐在高浓度时可转化为致癌化合物，从而对消费者构成严重的健康风险。为了减轻这一担忧，本研究利用原卟啉锌IX （ZnPP）作为一种安全有效的亚硝酸盐替代品。选择6株葡萄球菌，系统评价其促进ZnPP形成和优化肉糜模型体系颜色发展的能力。所有菌株的胞内铁螯合酶活性都明显高于胞外，其中equorum葡萄球菌表现出最明显的活性（P < 0.05）。与对照组相比，所有接种样品的L∗值显著升高，b∗值显著降低（P < 0.05）。荧光光谱显示，随着时间的推移，葡萄球菌接种组的znpp特异性吸收峰逐渐增强。在终点，接种牡参的肉末模型体系ZnPP含量最高（893.91±7.14 ng/g）。虽然低于亚硝酸盐处理的样品，但在终点处，与对照组相比，刺荆芥接种样品的a∗值显著升高（P < 0.05）。综上所述，在肉糜模型系统中，骏马草最有效地促进了ZnPP的形成和颜色的改善，将其定位为肉制品中完全/部分亚硝酸盐替代的可行着色策略。

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引用次数: 0

Bile resistance mechanisms in probiotics: From structural adaptation to metabolic regulation 益生菌胆阻机制：从结构适应到代谢调节

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-10 DOI: 10.1016/j.fbio.2026.108282

Jing-Wei Hu , Shu-Jun Guo , Lan-Wei Zhang , Chun-Feng Guo

Bile acids, amphipathic molecules synthesized in the liver and modified by gut microbiota, play dual roles as digestive surfactants and antimicrobial agents, significantly shaping the gut microbial ecosystem. This review elucidates bile acid-mediated antimicrobial mechanisms against probiotics and the sophisticated resistance strategies employed by these beneficial microbes. Human bile acid metabolism, encompassing biosynthesis via cytochrome P450 pathways and enterohepatic circulation, establishes dynamic chemical gradients in the intestinal tract. The antimicrobial effects of bile acids arise from membrane destabilization, cytoplasmic acidification, intracellular proteostasis imbalance, and oxidative stress induction. In response, probiotics deploy multilayered defense mechanisms, including active efflux pumps, bile salt hydrolase-mediated deconjugation, two-component signal transduction systems, F₁F₀-ATPase-driven pH regulation, and proteostasis networks. Structural adaptations such as membrane lipid remodeling, surface-layer protein barriers, and exopolysaccharide encapsulation further enhance bile resistance. Furthermore, the limitations in understanding these bile acid resistance mechanisms are discussed, and potential future research directions are suggested. These insights guide probiotic selection and optimization, enhancing their potential to confer health benefits in human applications.

胆汁酸是由肝脏合成并经肠道菌群修饰的两亲性分子，具有消化表面活性剂和抗菌剂的双重作用，对肠道微生物生态系统的塑造具有重要意义。本文综述了胆汁酸介导的抗益生菌机制以及这些有益微生物采用的复杂耐药策略。人体胆汁酸代谢，包括通过细胞色素P450途径和肠肝循环的生物合成，在肠道中建立动态化学梯度。胆汁酸的抗菌作用源于膜不稳定、细胞质酸化、细胞内蛋白酶平衡失衡和氧化应激诱导。作为回应，益生菌部署了多层防御机制，包括主动外排泵、胆汁盐水解酶介导的解偶联、双组分信号转导系统、f1f0 - atp酶驱动的pH调节和蛋白酶平衡网络。结构适应，如膜脂重塑、表层蛋白屏障和外多糖包封，进一步增强胆汁抵抗。此外，还讨论了对这些胆汁酸耐药机制的认识的局限性，并提出了未来可能的研究方向。这些见解指导益生菌的选择和优化，增强其在人类应用中赋予健康益处的潜力。

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引用次数: 0

Exploring the mechanism of lipid rancidity of Trichosanthes kirilowii Seeds during storage based on non-targeted lipidomics 基于非靶向脂质组学的栝槐种子贮藏过程中脂质酸败机理研究

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-10 DOI: 10.1016/j.fbio.2026.108278

Lixuan Chen , Zhiwei Liu , Weiwen Li , Pan Li , Bing Du , Lu Li

As a medicinal-edible resource, Trichosanthes kirilowii seeds are susceptible to lipid rancidity due to high unsaturated fatty acid content, yet the underlying mechanisms remain unclear. This study employed lipidomics to explore the mechanism driving lipid rancidity in Trichosanthes kirilowii seeds during three years of storage at 4 °C. Results indicate that with prolonged storage duration, key oxidation indicators (peroxide value, acid value, and malondialdehyde, etc.) and bitter amino acid content of Trichosanthes kirilowii seeds significantly increased, while umami and sweet amino acid content, along with the oleic-to-linoleic acid ratio, decreased. This confirms a sustained deterioration in quality. Hexanal, pentanal, and 1-pentanol were identified as potential biomarkers for assessing rancidity. Lipidomics identified 670 lipid species, with multivariate analysis revealing significant alterations between fresh seeds and those stored for 3 years. Pathway enrichment analysis pinpointed glycerophospholipid, glycerolipid, and linoleic acid metabolism as the central pathways involved. This research provides foundational data for regulating the storage stability of Trichosanthes kirilowii seeds.

栝楼种子作为一种药食资源，由于其不饱和脂肪酸含量高，容易发生脂质酸败，但其机制尚不清楚。本研究采用脂质组学的方法，探讨了在4℃条件下贮藏3年的栝槐种子脂质酸败的机制。结果表明，随着贮藏时间的延长，栝槐种子的主要氧化指标（过氧化值、酸值、丙二醛等）和苦味氨基酸含量显著增加，鲜味和甜味氨基酸含量随油酸比的降低而降低。这证实了质量的持续恶化。己醛、戊醛和1-戊醇被确定为评估酸败的潜在生物标志物。脂质组学鉴定出670种脂质，多变量分析显示新鲜种子和储存3年的种子之间存在显著变化。途径富集分析指出，甘油磷脂、甘油脂和亚油酸代谢是参与的主要途径。本研究为调控丝瓜种子的贮藏稳定性提供了基础数据。

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引用次数: 0

Water soluble polysaccharides from the fruits of Lycium barbarum L. improve glucose and lipid metabolism and boost immune function in elderly mice fed a high-fat diet 枸杞果实水溶性多糖可改善高脂饮食老年小鼠的糖脂代谢，增强免疫功能

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-10 DOI: 10.1016/j.fbio.2026.108280

Wanyi Wang , Haiyu Shi , Meng Zhang , Jia Mi , Lu Lu , Bo Jin , Lutao Zhang , Abdul Rehman , Chunhong Liu , Yamei Yan , Linwu Ran

Goji berry (Lycium barbarum L.), a traditional Chinese herbal remedy and functional food, contains polysaccharides (LBP) as its main active constituents. LBP has been shown to improve blood glucose and immune function. However, its effects under ageing conditions, particularly with high-fat diet (HFD) consumption, remain unclear. Herein, we evaluated the effects of water-soluble LBP on glucose and lipid metabolism and on immune function in elderly mice fed a high-fat diet. 2-month-old and 17-month-old female C57BL/6 mice were used. The mice were assigned to 5 groups: young, ageing, ageing +0.3 % LBP, ageing + HFD, and ageing + HFD +0.3 % LBP. The results showed that 0.3 % LBP extract treatment significantly reduced fasting blood glucose, insulin, total cholesterol, and low-density lipoprotein cholesterol levels and improved glucose tolerance in HFD-fed ageing mice. Histological and immunohistochemical analysis demonstrated that LBP extract ameliorated hepatic lipid accumulation and adipocyte hypertrophy and increased GLUT4 expression in adipocytes. Flow cytometry analysis demonstrated that in HFD-fed ageing mice, LBP extract intervention significantly decreased the proportion of peripheral MDSCs and increased the CD4⁺/CD8⁺ ratio. Additionally, correlation analysis revealed significant associations among fasting blood glucose, MDSCs proportion, and CD4⁺/CD8⁺ ratio. These findings highlight the preventive effect of LBP on aging-associated metabolic and immune dysfunction.

枸杞（Lycium barbarum L.）是一种传统的中药和功能性食品，其主要活性成分是多糖（LBP）。LBP已被证明可以改善血糖和免疫功能。然而，它在衰老条件下的影响，特别是高脂肪饮食（HFD）的消耗，仍不清楚。在此，我们评估了水溶性枸杞多糖对高脂饮食老年小鼠糖脂代谢和免疫功能的影响。选用2月龄和17月龄雌性C57BL/6小鼠。将小鼠分为5组：年轻组、衰老组、衰老+ 0.3% LBP组、衰老+ HFD组、衰老+ HFD + 0.3% LBP组。结果表明，0.3%枸杞多糖提取物显著降低了hfd喂养的衰老小鼠的空腹血糖、胰岛素、总胆固醇和低密度脂蛋白胆固醇水平，并改善了葡萄糖耐量。组织学和免疫组织化学分析表明，枸杞多糖提取物改善了肝脏脂质积累和脂肪细胞肥大，增加了脂肪细胞中GLUT4的表达。流式细胞术分析显示，LBP提取物干预显著降低hfd喂养的衰老小鼠外周血MDSCs比例，提高CD4+/CD8+比值。此外，相关分析显示空腹血糖、MDSCs比例和CD4+/CD8+比值存在显著相关性。这些发现强调了LBP对衰老相关代谢和免疫功能障碍的预防作用。

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引用次数: 0

Protective effects of Alaria esculenta and Palmaria palmata extracts on intestinal epithelial cells: insights from cell-based assays and transcriptomics 阿拉麦草和棕榈叶提取物对肠上皮细胞的保护作用：基于细胞的分析和转录组学的见解

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-10 DOI: 10.1016/j.fbio.2026.108281

Pierre Liboureau , Klevia Dishnica , Agnes Mols-Mortensen , Daniela Maria Pampanin

Seaweeds contain diverse bioactive compounds and are promising candidates for functional foods and nutraceuticals, though many studies lack in vivo relevance. This research used cell-based assays and transcriptomics to assess the effects of digested extracts from dried Alaria esculenta (Phaeophyceae), Palmaria palmata (Rhodophyta), and combinations of the two on human intestinal epithelium health. Caco-2 cells were exposed to seaweed extracts obtained through in vitro digestion and tested for viability, reactive oxygen species (ROS) production, wound healing, and IL-8 inflammatory response. At realistic intake concentrations (0.5–2 g), all extracts significantly inhibited ROS production induced by external stress. Cell viability was significantly increased by both species and their 1:1 mixture. No changes in anti-inflammatory or wound healing activities were recorded. Transcriptomics analysis revealed species-specific molecular response: A. esculenta activated cell defence pathways, while P. palmata enhanced nutrient transport and cellular functions. These differences suggest a potential complementary aspect of the two species for intestinal health, although analyses of the mixture effects suggest they may be antagonistic rather than synergistic. Small, significant differences between Norwegian and Faroese seaweed extracts suggest population and environmental effects on bioactive properties. Overall, both species showed protective properties against oxidative stress and may be good candidates as intestinal health supplements.

海藻含有多种生物活性化合物，是功能性食品和营养保健品的有希望的候选者，尽管许多研究缺乏体内相关性。本研究使用基于细胞的检测和转录组学来评估干燥的阿拉木叶（Phaeophyceae）， Palmaria palmata （Rhodophyta）的消化提取物，以及两者的组合对人类肠道上皮健康的影响。Caco-2细胞暴露于通过体外消化获得的海藻提取物中，并测试其活力、活性氧（ROS）产生、伤口愈合和IL-8炎症反应。在实际摄入浓度（0.5-2 g）下，所有提取物均能显著抑制外部应激诱导的ROS生成。两种菌种及其1:1混合物均显著提高了细胞活力。抗炎和伤口愈合活性均无变化。转录组学分析揭示了物种特异性的分子反应：马蹄莲激活了细胞防御途径，而马蹄莲则增强了营养转运和细胞功能。这些差异表明，这两个物种对肠道健康具有潜在的互补作用，尽管对混合效应的分析表明，它们可能是拮抗的，而不是协同的。挪威和法罗海藻提取物之间微小而显著的差异表明种群和环境对生物活性特性的影响。总的来说，这两种植物都显示出抗氧化应激的保护作用，可能是肠道健康补充剂的良好候选者。

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引用次数: 0

Lactiplantibacillus plantarum JLAU103 fermented walnut-milk dual-protein yogurt: Physicochemical property, volatile flavor profile, antioxidant activity, and isolation of neuroprotective peptides targeting Aβ1-42 oligomers 植物乳杆菌JLAU103发酵核桃乳双蛋白酸奶：理化性质、挥发性风味特征、抗氧化活性和针对a - β1-42寡聚物的神经保护肽的分离

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-09 DOI: 10.1016/j.fbio.2026.108260

Lixia Ma, Dina Chang, Jiaxin Lv, Yan Yan, Anxu Zheng, Xuehang Wang, Yue Leng, Bin Jiang, Dayong Ren, Ji Wang

Walnuts are nutrient-dense with diverse physiological functions. This study developed a walnut-milk dual-protein yogurt (WY) using Lactiplantibacillus plantarum JLAU103, investigating its refrigerated storage (4 °C) properties, microstructure, volatile profile, sensory traits, antioxidant activity, and isolating antioxidant peptides. The WY exhibited excellent storage stability over a 21-day period, maintaining an optimal and stable pH range of 4.48–4.78 and titratable acidity of 63.3–72.66 °T. Compared with the MY control, WY showed markedly reduced syneresis, enhanced water-holding capacity, and superior textural and viscosity properties, while sustaining viable L. plantarum JLAU103 populations above 10⁷ CFU/mL. Rheological and microstructural analyses showed WY had higher elastic (G′)/viscous (G″) moduli and a denser protein network, enhancing gel stability. WY contained 200 volatile compounds, with higher aldehydes/furans (key for nutty/roasted aromas) and better sensory acceptability than MY. Ultrafiltration fractionated WY proteins into <3 kDa (superior antioxidant) and 3–10 kDa fractions; the <3 kDa fraction was purified to sub-fraction B1, which exhibited strong radical scavenging (ABTS⁺: 15.86 %, DPPH: 53.48 %, •OH: 48.21 %) and Fe²⁺ chelating (98.23 %) capacities. Nano-LC-MS/MS identified 1972 peptides in B1, 10 peptides with predicted antioxidant activity via BIOPEP-UWM. Peptide GPYHFR bound Aβ_1-42 oligomers (−7.7 kcal/mol), showing potential to mitigate memory impairment. This study provides a theoretical/technical basis for high-quality functional walnut fermented dairy products and walnut bioactive peptide exploitation.

核桃营养丰富，具有多种生理功能。本研究利用植物乳杆菌JLAU103制备了核桃乳双蛋白酸奶（WY），研究了其冷藏（4°C）特性、微观结构、挥发性特征、感官特性、抗氧化活性和抗氧化肽的分离。在21天内，WY表现出良好的储存稳定性，保持了4.48-4.78的最佳稳定pH范围和63.3-72.66°T的可滴定酸度。与MY对照相比，WY显著降低了植物L. plantarum JLAU103的协同作用，增强了保水能力，并具有优越的结构和黏度特性，使植物L. plantarum JLAU103的活菌数量维持在107 CFU/mL以上。流变学和微观结构分析表明，WY具有更高的弹性（G′）/粘性（G″）模量和更致密的蛋白质网络，增强了凝胶的稳定性。WY含有200种挥发性化合物，具有较高的醛类/呋喃（坚果/烘烤香气的关键），并且比MY具有更好的感官接受度。超滤将WY蛋白分成3 kDa（优质抗氧化剂）和3 - 10 kDa两部分；3 kDa组分纯化为B1亚组分，具有较强的自由基清除能力（ABTS+: 15.86%, DPPH: 53.48%，•OH: 48.21%）和Fe2+螯合能力（98.23%）。通过BIOPEP-UWM，纳米lc -MS/MS鉴定了B1中1972个肽类，其中10个肽类具有预测的抗氧化活性。肽GPYHFR结合a - β1-42寡聚物（−7.7 kcal/mol），显示出减轻记忆障碍的潜力。本研究为优质功能性核桃发酵乳制品和核桃活性肽的开发提供了理论/技术依据。

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引用次数: 0

Interactive effects of abiotic factors on the ecophysiology characteristics of Aspergillus flavus under future climate conditions 未来气候条件下非生物因子对黄曲霉生理生态特性的交互影响

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience

Pub Date : 2026-01-09 DOI: 10.1016/j.fbio.2026.108272

Xuran Fu , Jiadi Sun , Honglin Yan , Xiyu Cao , Chuanzhi Jiang , Jin Ye , Wei Wang , Jia-Sheng Wang , Xiulan Sun

Climate change could affect the growth and colonization of Aspergillus flavus (A. flavus) in maize, increasing the risk of mycotoxins in aflatoxins (AFs) and cyclopiazonic acid (CPA). However, changes in ecophysiological characteristics of A. flavus under future climate scenarios remain unclear, particularly under alternating temperatures resulting from day and night variations. This study aims to explore the interactive effects of alternating temperatures (28/20 °C and 33/25 °C) under the photoperiod with 13 h light/11h darkness, CO₂ (400 ppm and 1000 ppm), and different water activities (0.93 aw, 0.95 aw and 0.99 aw). The growth rate, lag phase, conidia production, oxidative stress indicators and related mycotoxins (AFs and CPA) of A. flavus were analyzed under a combination of different climatic factors in maize flour agar (MFA) and potato dextrose agar (PDA) medium. The results indicate that water activity is the primary factor regulating A. flavus growth, conidia production, and mycotoxin production. Its single-factor effects and interactions with other factors exert a highly significant influence on all indicators (p < 0.001). Elevated alternating temperatures promote the growth of A. flavus. The impact of CO₂ is relatively limited, with significant effects observed only on conidia and CPA. The interaction between alternating temperature, water activity, and CO₂ concentration have an effect on growth and mycotoxin production in A. flavus. However, mycotoxin production does not fully correlate with gene expression. These findings provide valuable baseline data on the interactions between temperature, water activity, and CO₂ concentration in relation to A. flavus growth and mycotoxin production.

气候变化可能影响玉米中黄曲霉（Aspergillus flavus）的生长和定植，增加黄曲霉毒素（AFs）和环吡唑酸（CPA）真菌毒素的风险。然而，黄曲霉的生理生态特性在未来气候情景下的变化尚不清楚，特别是在昼夜交替的温度变化下。本研究旨在探讨在光周期（13 h光照/11h黑暗）下交替温度（28/20°C和33/25°C）、CO2 （400 ppm和1000 ppm）和不同水活度（0.93 aw、0.95 aw和0.99 aw）的交互效应。研究了不同气候条件下黄曲霉在玉米粉琼脂（MFA）和马铃薯葡萄糖琼脂（PDA）培养基中的生长速率、滞后期、分生孢子产量、氧化胁迫指标及相关真菌毒素（AFs和CPA）。结果表明，水分活性是调节黄曲霉生长、分生孢子产生和霉菌毒素产生的主要因素。它的单因素效应和与其他因素的相互作用对所有指标都有非常显著的影响（p < 0.001）。升高的交替温度促进黄曲霉的生长。CO2的影响相对有限，仅对分生孢子和CPA有显著影响。交变温度、水分活度和CO2浓度的交互作用对黄曲霉的生长和霉菌毒素的产生有影响。然而，霉菌毒素的产生并不完全与基因表达相关。这些发现为温度、水分活度和CO2浓度与黄曲霉生长和霉菌毒素产生之间的相互作用提供了有价值的基线数据。

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引用次数: 0