The preharvest internal browning of Nane plum fruit, with no visible effects on the appearance of the fruit, has become a serious problem in recent years in its production area in Guangdong Province, China. This study investigated the effects of environmental factors, including temperature, on Nane plum internal browning. Plum orchards at different elevations with different incidences of internal browning were selected. Using fruits with different internal browning incidence levels, the internal browning mechanism was analyzed with transcriptome and metabolome analyses. The results revealed decreased internal browning at high altitudes. Shading treatment significantly reduced internal browning, whereas bagging and insect-proof net-covering treatments significantly increased internal browning. Because bagging and net coverings increase the local ambient temperature, the findings suggest that high temperature is an important factor influencing the internal browning of Nane plum. The metabolome experiments showed that with increased internal browning, the levels of phenolic hydroxyls such as catechol increased, with simultaneous increases in hydrogen peroxide content and oxidase activity. It can be speculated that the oxidation of phenolic hydroxyl substances is the main cause of the preharvest browning of Nane plum. Transcriptome analysis revealed the increased expression of calcium signaling-related and downstream effector genes and indicated an important role of calcium in internal browning, possibly due to its increased content in the fruit. Further, with increasingly serious internal browning, genes related to photosynthesis were down-regulated, while genes related to senescence were up-regulated, thus suggesting the up-regulation of the process of cell senescence during internal browning. In conclusion, heat stress should be eliminated to reduce preharvest internal browning in Nane plum.
{"title":"Transcriptome and metabolome analysis of preharvest internal browning in Nane plum fruit caused by high temperatures","authors":"Cheng Peng, Linping Deng, Hejun Tan, Wancong Meng, Jianliang Luo, Zengwen Zhang, Huiqiong Chen, Jishui Qiu, Xiaoxiao Chang, Yusheng Lu","doi":"10.1016/j.hpj.2023.10.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.10.004","url":null,"abstract":"The preharvest internal browning of Nane plum fruit, with no visible effects on the appearance of the fruit, has become a serious problem in recent years in its production area in Guangdong Province, China. This study investigated the effects of environmental factors, including temperature, on Nane plum internal browning. Plum orchards at different elevations with different incidences of internal browning were selected. Using fruits with different internal browning incidence levels, the internal browning mechanism was analyzed with transcriptome and metabolome analyses. The results revealed decreased internal browning at high altitudes. Shading treatment significantly reduced internal browning, whereas bagging and insect-proof net-covering treatments significantly increased internal browning. Because bagging and net coverings increase the local ambient temperature, the findings suggest that high temperature is an important factor influencing the internal browning of Nane plum. The metabolome experiments showed that with increased internal browning, the levels of phenolic hydroxyls such as catechol increased, with simultaneous increases in hydrogen peroxide content and oxidase activity. It can be speculated that the oxidation of phenolic hydroxyl substances is the main cause of the preharvest browning of Nane plum. Transcriptome analysis revealed the increased expression of calcium signaling-related and downstream effector genes and indicated an important role of calcium in internal browning, possibly due to its increased content in the fruit. Further, with increasingly serious internal browning, genes related to photosynthesis were down-regulated, while genes related to senescence were up-regulated, thus suggesting the up-regulation of the process of cell senescence during internal browning. In conclusion, heat stress should be eliminated to reduce preharvest internal browning in Nane plum.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140038116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Inducing adventitious root (AR) formation in mature walnut species ( L.) is challenging. However, the AR formation of mature trees can be improved by rejuvenation. In rejuvenated cuttings, exogenous indole-3-butyric acid (IBA) is essential for AR formation, and the underlying mechanism is still not well understood. Therefore, we utilized transcriptome sequencing to investigate the mechanism of IBA-induced AR formation. Our results revealed that, in comparison to the control group, IBA treatment (9 mM) significantly increased the endogenous indole-3-acetic acid (IAA) content, leading to an enhanced rooting rate. We performed RNA sequencing to identify differentially expressed genes (DEGs) between the IBA-treated and control (CK) groups at 1, 2, 3, and 5 days after cutting (DAC). The results showed that, compared to the control cuttings, there were 1 539, 889, 785, and 984 up-regulated genes and 2 791, 2 936, 3 017, and 1 752 down-regulated genes, at 1, 2, 3, and 5 DAC, respectively. Analysis of RNA-seq data revealed that (/) and (), associated with IBA transport, were down-regulated in the rejuvenation cuttings. In contrast, () and (), associated with auxin efflux, were up-regulated. We identified 49 ()-encoding genes, including , , , , , , , , and , which were up-regulated at 1–5 DAC in the rejuvenated cuttings. This study highlights that the overexpression of in poplar significantly enhance AR growth, as evidenced by increased root length, surface area, volume, and quantity. Moreover, the co-expression network analysis involving and in walnut cuttings elucidates complex genetic interactions, underscoring their pivotal role in the formation of AR. Our data supported the following molecular mechanism of IBA-induced adventitious root formation. Firstly, IBA is converted to free IAA in peroxisomes. Then, the highly concentrated IAA in the procambium and parenchyma cells induces
{"title":"Transcriptome profiling of Indole-3-Butyric Acid-Induced Adventitious Root Formation in softwood Cuttings of walnut","authors":"Xiaobo Song, Ruimin Huang, Hao Liu, Zhang Junpei, Yingying Chang, Dong Pei","doi":"10.1016/j.hpj.2023.04.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.04.013","url":null,"abstract":"Inducing adventitious root (AR) formation in mature walnut species ( L.) is challenging. However, the AR formation of mature trees can be improved by rejuvenation. In rejuvenated cuttings, exogenous indole-3-butyric acid (IBA) is essential for AR formation, and the underlying mechanism is still not well understood. Therefore, we utilized transcriptome sequencing to investigate the mechanism of IBA-induced AR formation. Our results revealed that, in comparison to the control group, IBA treatment (9 mM) significantly increased the endogenous indole-3-acetic acid (IAA) content, leading to an enhanced rooting rate. We performed RNA sequencing to identify differentially expressed genes (DEGs) between the IBA-treated and control (CK) groups at 1, 2, 3, and 5 days after cutting (DAC). The results showed that, compared to the control cuttings, there were 1 539, 889, 785, and 984 up-regulated genes and 2 791, 2 936, 3 017, and 1 752 down-regulated genes, at 1, 2, 3, and 5 DAC, respectively. Analysis of RNA-seq data revealed that (/) and (), associated with IBA transport, were down-regulated in the rejuvenation cuttings. In contrast, () and (), associated with auxin efflux, were up-regulated. We identified 49 ()-encoding genes, including , , , , , , , , and , which were up-regulated at 1–5 DAC in the rejuvenated cuttings. This study highlights that the overexpression of in poplar significantly enhance AR growth, as evidenced by increased root length, surface area, volume, and quantity. Moreover, the co-expression network analysis involving and in walnut cuttings elucidates complex genetic interactions, underscoring their pivotal role in the formation of AR. Our data supported the following molecular mechanism of IBA-induced adventitious root formation. Firstly, IBA is converted to free IAA in peroxisomes. Then, the highly concentrated IAA in the procambium and parenchyma cells induces","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140038123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An association analysis of lipidome and transcriptome highlights the involvement of MmGDPD1 in regulating low phosphorus tolerance in Malus mandshurica","authors":"Hong-yuan Zhao, Yawei Wu, Luonan Shen, Zhengchun Li, Lin Deng, Xiaopeng Wen","doi":"10.1016/j.hpj.2024.01.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.01.006","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140405189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-29DOI: 10.1016/j.hpj.2023.06.006
Esther Gimeno-Páez, Jaime Prohens, María Moreno-Cerveró, Ana de Luis-Margarit, María José Díez, Pietro Gramazio
Unlike other major crops, little research has been performed on tomato to reduce the generation time for speed breeding. We evaluated several agronomic treatments to reduce the generation time of tomato in the ‘M82’ (determinate) and ‘Moneymaker’ (indeterminate) varieties and evaluated the best combination in conjunction with embryo rescue. Five container sizes with volumes of 0.2 L (XS), 0.45 L (S), 0.8 L (M), 1.3 L (L), and 6 L (XL), were evaluated in the first experiment under the autumn cycle. We found that plants grown in XL containers exhibited better development and required less time from sowing to anthesis (DSA) and from anthesis to fruit ripening (DAR). In the second experiment, using XL containers in the autumn–winter cycle, we evaluated the effects of cold priming at the cotyledonary stage, water stress, P supplementation, and K supplementation on generation time. Compared to the control, we found that cold priming significantly reduced the number of leaves, plant height to first the inflorescence, and DSA (2.7 d), whereas K supplementation reduced the DAR (8.8 d). In contrast, water stress and P supplementation did not significantly affect any of the measured traits like DAR, DSA or fruit set. To validate these data, in a third experiment with XL containers in the spring-summer cycle, the combination of cold priming and K supplementation was tested, confirming the significant effect of this combination on the reduction of generation time (2.9 d for DSA and 3.9 d for DAR) compared to the control. Embryo rescue during the cell expansion cycle (average of 22.0 d and 23.3 d after anthesis for ‘M82’ and ‘Moneymaker’, respectively) allowed the shortening of the generation time by 8.7 d in ‘M82’ and 11.6 d in ‘Moneymaker’ compared to the fruit ripening. The combination of agronomic treatments with embryo rescue can effectively increase the number of generations per year from three to four for speed breeding of tomato.
{"title":"Agronomic treatments combined with embryo rescue for rapid generation advancement in tomato speed breeding","authors":"Esther Gimeno-Páez, Jaime Prohens, María Moreno-Cerveró, Ana de Luis-Margarit, María José Díez, Pietro Gramazio","doi":"10.1016/j.hpj.2023.06.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.06.006","url":null,"abstract":"Unlike other major crops, little research has been performed on tomato to reduce the generation time for speed breeding. We evaluated several agronomic treatments to reduce the generation time of tomato in the ‘M82’ (determinate) and ‘Moneymaker’ (indeterminate) varieties and evaluated the best combination in conjunction with embryo rescue. Five container sizes with volumes of 0.2 L (XS), 0.45 L (S), 0.8 L (M), 1.3 L (L), and 6 L (XL), were evaluated in the first experiment under the autumn cycle. We found that plants grown in XL containers exhibited better development and required less time from sowing to anthesis (DSA) and from anthesis to fruit ripening (DAR). In the second experiment, using XL containers in the autumn–winter cycle, we evaluated the effects of cold priming at the cotyledonary stage, water stress, P supplementation, and K supplementation on generation time. Compared to the control, we found that cold priming significantly reduced the number of leaves, plant height to first the inflorescence, and DSA (2.7 d), whereas K supplementation reduced the DAR (8.8 d). In contrast, water stress and P supplementation did not significantly affect any of the measured traits like DAR, DSA or fruit set. To validate these data, in a third experiment with XL containers in the spring-summer cycle, the combination of cold priming and K supplementation was tested, confirming the significant effect of this combination on the reduction of generation time (2.9 d for DSA and 3.9 d for DAR) compared to the control. Embryo rescue during the cell expansion cycle (average of 22.0 d and 23.3 d after anthesis for ‘M82’ and ‘Moneymaker’, respectively) allowed the shortening of the generation time by 8.7 d in ‘M82’ and 11.6 d in ‘Moneymaker’ compared to the fruit ripening. The combination of agronomic treatments with embryo rescue can effectively increase the number of generations per year from three to four for speed breeding of tomato.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140038143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-29DOI: 10.1016/j.hpj.2023.07.008
Jinita Sthapit Kandel, Ivan Simko, Ryan J. Hayes, Beiquan Mou
Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP). Even in MAP, fresh-cut lettuce has short shelf life that results in loss of nutrients. Lettuce cultivars exhibit genetic variation for shelf life in MAP, but their variation for nutrient retention is not known. Fifty accessions were evaluated for initial content of ascorbic acid (AsA), carotenoids, and sugars and their retention in storage. Accessions with high content and/or good retention of one or more nutrients were identified. The romaine accession ‘Floricos’ had high levels of all the three nutrients. Accessions with relatively high retention of all the three nutrients were ‘Salinas 88’, ‘Siskiyou’, ‘Solar’, SM09A, ‘Romance’, and ‘Green Towers’. Romaine cultivars, ‘Balady Barrage’, ‘Green Towers’, and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their retention. There was no clear correlation between initial AsA/carotene concentrations and their retention rates, suggesting that besides content, retention of nutrients should also be a breeding target in a lettuce nutritional improvement program. Statistical analyses with the Pearson’s correlation coefficient determined a negative relationship between tissue deterioration (AUDePS) and retention of all tested nutrients [ of -0.52 ( < 0.0001) for AsA, -0.27 ( < 0.01) for total carotene, and -0.59 ( < 0.0001) for total sugars], suggesting that an increase in tissue deterioration intensifies nutrient decay. Broad-sense heritability () across the experiments was 0.15 for AsA, 0.23 for total carotene, and 0.50 for total sugars. Identification of germplasm with high nutrient content, extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.
{"title":"Concentration and retention of ascorbic acid, carotenoids, and sugars in fresh-cut lettuce in modified atmosphere packaging","authors":"Jinita Sthapit Kandel, Ivan Simko, Ryan J. Hayes, Beiquan Mou","doi":"10.1016/j.hpj.2023.07.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.07.008","url":null,"abstract":"Fresh-cut lettuce is widely used in ready-to-eat salads sold in modified atmosphere packages (MAP). Even in MAP, fresh-cut lettuce has short shelf life that results in loss of nutrients. Lettuce cultivars exhibit genetic variation for shelf life in MAP, but their variation for nutrient retention is not known. Fifty accessions were evaluated for initial content of ascorbic acid (AsA), carotenoids, and sugars and their retention in storage. Accessions with high content and/or good retention of one or more nutrients were identified. The romaine accession ‘Floricos’ had high levels of all the three nutrients. Accessions with relatively high retention of all the three nutrients were ‘Salinas 88’, ‘Siskiyou’, ‘Solar’, SM09A, ‘Romance’, and ‘Green Towers’. Romaine cultivars, ‘Balady Barrage’, ‘Green Towers’, and ‘Darkland’ had relatively high initial levels of all tested nutrients and good rate of their retention. There was no clear correlation between initial AsA/carotene concentrations and their retention rates, suggesting that besides content, retention of nutrients should also be a breeding target in a lettuce nutritional improvement program. Statistical analyses with the Pearson’s correlation coefficient determined a negative relationship between tissue deterioration (AUDePS) and retention of all tested nutrients [ of -0.52 ( < 0.0001) for AsA, -0.27 ( < 0.01) for total carotene, and -0.59 ( < 0.0001) for total sugars], suggesting that an increase in tissue deterioration intensifies nutrient decay. Broad-sense heritability () across the experiments was 0.15 for AsA, 0.23 for total carotene, and 0.50 for total sugars. Identification of germplasm with high nutrient content, extended shelf life and good nutrient retention provides valuable information for the lettuce industry and associated breeding programs.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140038119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：