Pub Date : 2025-10-28DOI: 10.1016/j.hpj.2025.06.018
Yuanpeng Fang, Zehui Wang, Salah F. Abou-Elwafa, Maha Aljabri, Xin Xie
{"title":"Interplay of Cytochrome P450 genes and flavonoid pathways in enhancing plant defense against heavy metal toxicity","authors":"Yuanpeng Fang, Zehui Wang, Salah F. Abou-Elwafa, Maha Aljabri, Xin Xie","doi":"10.1016/j.hpj.2025.06.018","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.06.018","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"26 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145383268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Nutrient Expert system improves nutrient use efficiency and reduces apparent nutrient residues of tomatoes in soil of different fertility in North China","authors":"Binggeng Yang, Jiwen Cui, Mengjiao Liu, Kangrui Fang, Chao Ai, Wencheng Ding, Xinpeng Xu, Ping He, Dali Song, Wei Zhou","doi":"10.1016/j.hpj.2025.07.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.07.012","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"27 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145383273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomato (Solanum lycopersicum L.) is an important horticultural crop, but it is seriously affected by salt stress. GR24, the synthetic analogue of Strigolactones (SLs) is used in the study as an efficient and harmless growth regulator. Therefore, further exploration of the mechanism of exogenous SLs is necessary to improve tomato salt tolerance. In this study, RNA-seq analysis, sulfite whole genome sequencing and methylation-specific PCR were used to conduct salt stress analysis on the leaf samples of the tomato variety "Micro-Tom" seedlings treated with exogenous SLs. Research shows that exogenous SLs alleviate the inhibition of the number of leaves, root surface area and root volume resulting from salt stress. Compared with the 150 mmol·L−1 NaCl treatment, 150 mmol·L−1 NaCl +15 μmol·L−1 GR24 treatment increases the leaf number, root surface area and root volume by 26.67 %, 55.76 % and 55.81 %, respectively, suggesting that exogenous SLs-mediated DNA demethylation may play an important role in the salt tolerance of the four-leaf stage tomato seedlings. RNA-sequencing and genome-wide methylation analysis show that exogenous SLs reduce DNA methylation levels to affect phenylalanine metabolism and phenylpropanoid biosynthesis under salt stress. SLs-mediated DNA demethylation increases phenethylamine (PEA), coumarin, caffeic acid, and lignin contents, but decreases l-Phenylalanine (LPA) and cinnamic acid (CA) contents. Meanwhile, the activities of l-phenylalanine ammonia-lyase (PAL), phenylalanine decarboxylase (HDC), 5-O-(4-coumaroyl)-d-quinate 3′-monooxygenase (CYP98A3), and beta-glucosidase (BGLU) are enhanced, and 6 genes related to phenylpropanoid metabolism (SlPAL5, SlHDC, SlBGLU41, SlCYP98A3, SlCYP73A4, and Sl4CLL7) in the pathway were induced. The demethylation of SlCYP98A3 at CG site in promoter, SlBGLU41 at CG site in gene body, SlPAL5 at CHG site in gene body, SlPAL at CHG site in promoter and SlHDC at CHG site in promoter may result in the transcription of the genes, activating other genes expression. These findings demonstrate that exogenous SLs may improve the salt tolerance of tomato seedlings by regulating phenylalanine metabolism and phenylpropanoid biosynthesis. The results provide a reference for in-depth analysis of the response mechanism of SLs under abiotic stress.
{"title":"Strigolactone-mediated DNA demethylation induces phenylpropanoid biosynthesis to alleviate salt stress in tomato","authors":"Changxia Li, Yuxia Yao, Xiaping Gong, Xuefang Lu, Shaoxia Li, Wenjin Yu","doi":"10.1016/j.hpj.2025.06.015","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.06.015","url":null,"abstract":"Tomato (<ce:italic>Solanum lycopersicum</ce:italic> L.) is an important horticultural crop, but it is seriously affected by salt stress. GR24, the synthetic analogue of Strigolactones (SLs) is used in the study as an efficient and harmless growth regulator. Therefore, further exploration of the mechanism of exogenous SLs is necessary to improve tomato salt tolerance. In this study, RNA-seq analysis, sulfite whole genome sequencing and methylation-specific PCR were used to conduct salt stress analysis on the leaf samples of the tomato variety \"Micro-Tom\" seedlings treated with exogenous SLs. Research shows that exogenous SLs alleviate the inhibition of the number of leaves, root surface area and root volume resulting from salt stress. Compared with the 150 mmol·L<ce:sup loc=\"post\">−1</ce:sup> NaCl treatment, 150 mmol·L<ce:sup loc=\"post\">−1</ce:sup> NaCl +15 μmol·L<ce:sup loc=\"post\">−1</ce:sup> GR24 treatment increases the leaf number, root surface area and root volume by 26.67 %, 55.76 % and 55.81 %, respectively, suggesting that exogenous SLs-mediated DNA demethylation may play an important role in the salt tolerance of the four-leaf stage tomato seedlings. RNA-sequencing and genome-wide methylation analysis show that exogenous SLs reduce DNA methylation levels to affect phenylalanine metabolism and phenylpropanoid biosynthesis under salt stress. SLs-mediated DNA demethylation increases phenethylamine (PEA), coumarin, caffeic acid, and lignin contents, but decreases <ce:small-caps>l</ce:small-caps>-Phenylalanine (LPA) and cinnamic acid (CA) contents. Meanwhile, the activities of <ce:small-caps>l</ce:small-caps>-phenylalanine ammonia-lyase (PAL), phenylalanine decarboxylase (HDC), 5-O-(4-coumaroyl)-<ce:small-caps>d</ce:small-caps>-quinate 3′-monooxygenase (CYP98A3), and beta-glucosidase (BGLU) are enhanced, and 6 genes related to phenylpropanoid metabolism (<ce:italic>SlPAL5</ce:italic>, <ce:italic>SlHDC</ce:italic>, <ce:italic>SlBGLU41</ce:italic>, <ce:italic>SlCYP98A3</ce:italic>, <ce:italic>SlCYP73A4</ce:italic>, and <ce:italic>Sl4CLL7</ce:italic>) in the pathway were induced. The demethylation of <ce:italic>SlCYP98A3</ce:italic> at CG site in promoter, <ce:italic>SlBGLU41</ce:italic> at CG site in gene body, <ce:italic>SlPAL5</ce:italic> at CHG site in gene body, <ce:italic>SlPAL</ce:italic> at CHG site in promoter and <ce:italic>SlHDC</ce:italic> at CHG site in promoter may result in the transcription of the genes, activating other genes expression. These findings demonstrate that exogenous SLs may improve the salt tolerance of tomato seedlings by regulating phenylalanine metabolism and phenylpropanoid biosynthesis. The results provide a reference for in-depth analysis of the response mechanism of SLs under abiotic stress.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"66 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145311717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-27DOI: 10.1016/j.hpj.2025.06.014
Shuo Ma, Tong Li, Ziquan Feng, Wenping Hou, Shunfeng Ge, Yali Zhang, Yanhui Lv, Han Jiang, Yuanyuan Li
The outermost protective layer of plant cells is known as the cell wall, and it mostly comprises cellulose, hemicellulose, and pectin. The primary component of the hemicellulose in the cell wall of higher plants is xyloglucan, which provides the cell wall with mechanical support and restricts cell growth. XTH gene family members contribute to the remodeling of plant cell walls by encoding proteins with glycosyltransferase/hydrolase activity, which can mediate the cleavage and rearrangement of xyloglucan chains. Plants can enhance their resilience to external stress by modifying the structure and composition of the cell wall. However, few studies have been conducted on the XTH gene family in apples. Here, we successfully isolated MdXTH15 from the apple genome and found that it contained a highly conserved GH16-XET domain. The expression of this gene was highest in the stem of the apple, and it responded to external abiotic stress treatment. The protein was found to be localized to the plasma membrane by subcellular localization analysis. Its overexpression enhanced abiotic stress and pathogen resistance in both apple and Arabidopsis. Furthermore, we elucidated the molecular mechanisms underlying stress resistance at the cell wall level and provided a novel perspective on this phenomenon. Overall, our findings provide a novel approach for enhancing the stress resistance of apples.
{"title":"Apple gene MdXTH15, encoding endotransferase/hydrolase for xyloglucan, can improve plant resistance to drought, salt, and pathogen stresses","authors":"Shuo Ma, Tong Li, Ziquan Feng, Wenping Hou, Shunfeng Ge, Yali Zhang, Yanhui Lv, Han Jiang, Yuanyuan Li","doi":"10.1016/j.hpj.2025.06.014","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.06.014","url":null,"abstract":"The outermost protective layer of plant cells is known as the cell wall, and it mostly comprises cellulose, hemicellulose, and pectin. The primary component of the hemicellulose in the cell wall of higher plants is xyloglucan, which provides the cell wall with mechanical support and restricts cell growth. <ce:italic>XTH</ce:italic> gene family members contribute to the remodeling of plant cell walls by encoding proteins with glycosyltransferase/hydrolase activity, which can mediate the cleavage and rearrangement of xyloglucan chains. Plants can enhance their resilience to external stress by modifying the structure and composition of the cell wall. However, few studies have been conducted on the <ce:italic>XTH</ce:italic> gene family in apples. Here, we successfully isolated <ce:italic>MdXTH15</ce:italic> from the apple genome and found that it contained a highly conserved GH16-XET domain. The expression of this gene was highest in the stem of the apple, and it responded to external abiotic stress treatment. The protein was found to be localized to the plasma membrane by subcellular localization analysis. Its overexpression enhanced abiotic stress and pathogen resistance in both apple and <ce:italic>Arabidopsis</ce:italic>. Furthermore, we elucidated the molecular mechanisms underlying stress resistance at the cell wall level and provided a novel perspective on this phenomenon. Overall, our findings provide a novel approach for enhancing the stress resistance of apples.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"30 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145311716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-27DOI: 10.1016/j.hpj.2025.05.018
Ke Wang, Baigeng Hu, Shiqi Wen, Philip James Kear, Lina Shang, Shiwei Chang, Dianqiu Lyu, Hongju Jian
Soil salinization is one of the most prominent abiotic stresses affecting agricultural production. As the third most significant staple crop, the potato exhibits heightened sensitivity to salt stress. Alternative polyadenylation (APA) is a key regulator of gene expression, significantly impacting plant growth and stress response. However, the role of APA in response to salt stress remains elusive in potato, as genetic resources for salt-tolerant potatoes are limited. In this study, germplasms of nine salt-sensitive and seven salt-tolerant accessions were screened, respectively. Salt-tolerant germplasms exhibited superior ROS scavenging capabilities and ionic balance compared to salt-sensitive germplasms. This study characterized APA events in leaves and roots of Morocco 1 (salt-tolerant) and Qingshu 9 (salt-sensitive) under control and salt stress using TAIL-seq. Salt stress induced global APA dynamics in potato. A total of 1 831 and 4 235 APA genes were identified in the leaves and roots of Qingshu 9, respectively. In contrast, Morocco 1 exhibited only 559 and 2 696 APA genes in its leaves and roots, respectively. APA led to an average extension of the 3’ UTR of most genes by 25 bp. Moreover, five candidate genes potentially responsive to salt stress via APA were identified. In summary, our results illustrate that APA is significant for regulating gene expression under salt stress, providing new perspectives for studying salt tolerance in potato.
{"title":"Screening of salt-tolerant potato germplasms and dynamic changes of APA in response to salt stress","authors":"Ke Wang, Baigeng Hu, Shiqi Wen, Philip James Kear, Lina Shang, Shiwei Chang, Dianqiu Lyu, Hongju Jian","doi":"10.1016/j.hpj.2025.05.018","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.05.018","url":null,"abstract":"Soil salinization is one of the most prominent abiotic stresses affecting agricultural production. As the third most significant staple crop, the potato exhibits heightened sensitivity to salt stress. Alternative polyadenylation (APA) is a key regulator of gene expression, significantly impacting plant growth and stress response. However, the role of APA in response to salt stress remains elusive in potato, as genetic resources for salt-tolerant potatoes are limited. In this study, germplasms of nine salt-sensitive and seven salt-tolerant accessions were screened, respectively. Salt-tolerant germplasms exhibited superior ROS scavenging capabilities and ionic balance compared to salt-sensitive germplasms. This study characterized APA events in leaves and roots of Morocco 1 (salt-tolerant) and Qingshu 9 (salt-sensitive) under control and salt stress using TAIL-seq. Salt stress induced global APA dynamics in potato. A total of 1 831 and 4 235 APA genes were identified in the leaves and roots of Qingshu 9, respectively. In contrast, Morocco 1 exhibited only 559 and 2 696 APA genes in its leaves and roots, respectively. APA led to an average extension of the 3’ UTR of most genes by 25 bp. Moreover, five candidate genes potentially responsive to salt stress via APA were identified. In summary, our results illustrate that APA is significant for regulating gene expression under salt stress, providing new perspectives for studying salt tolerance in potato.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"198 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145311720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although both abscisic acid (ABA) and methyl jasmonate (MeJA) play significant roles in regulating the development and quality of grape (Vitis vinifera L.) berries, the regulatory effects and mechanisms of the combined application of ABA and MeJA remain unclear. To further explore the optimal combination of these hormones for regulating the development of grape quality, combined ABA and MeJA treatments were carried out in this study, with ‘Jumeigui’ grape used as the material. The results indicated that the combined treatment of high-ABA and low-MeJA (HA + LM) increased the sugar-acid ratio, promoted the accumulation of phenolic substances in grape skins, and resulted in anthocyanin content 168.9 % higher than that of the control, significantly enhancing coloration. Additionally, the combined treatment of low-ABA and low-MeJA (LA + LM) was more conducive to the accumulation of phenols in grape, especially phenolic acid and resveratrol, as the total phenolic content increased by 38.96 % relative to that of the control. Moreover, the expressions of aroma-related genes were upregulated by the combined high-MeJA treatments. The combined treatment of high-ABA and high-MeJA (HA + HM) markedly increased terpene biosynthesis, followed by the LA + HM treatment, increasing the intensity of the rose flavor characteristics of the ‘Jumeigui’ grape. Therefore, the combination of MeJA and ABA at different concentrations had distinct effects on fruit quality and appropriate combinations can be selected according to the specific needs for the targeted metabolites.
{"title":"Effects of combined application of abscisic acid and methyl jasmonate on the regulation of anthocyanin and monoterpene biosynthesis in ‘Jumeigui’ grape","authors":"Zihan Zhang, Yujie Hu, Yangkang Zhang, Zhihao Deng, Li Chen, Wanping Li, Yulin Fang, Keqin Chen, Kekun Zhang","doi":"10.1016/j.hpj.2025.06.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.06.013","url":null,"abstract":"Although both abscisic acid (ABA) and methyl jasmonate (MeJA) play significant roles in regulating the development and quality of grape (<ce:italic>Vitis vinifera</ce:italic> L.) berries, the regulatory effects and mechanisms of the combined application of ABA and MeJA remain unclear. To further explore the optimal combination of these hormones for regulating the development of grape quality, combined ABA and MeJA treatments were carried out in this study, with ‘Jumeigui’ grape used as the material. The results indicated that the combined treatment of high-ABA and low-MeJA (HA + LM) increased the sugar-acid ratio, promoted the accumulation of phenolic substances in grape skins, and resulted in anthocyanin content 168.9 % higher than that of the control, significantly enhancing coloration. Additionally, the combined treatment of low-ABA and low-MeJA (LA + LM) was more conducive to the accumulation of phenols in grape, especially phenolic acid and resveratrol, as the total phenolic content increased by 38.96 % relative to that of the control. Moreover, the expressions of aroma-related genes were upregulated by the combined high-MeJA treatments. The combined treatment of high-ABA and high-MeJA (HA + HM) markedly increased terpene biosynthesis, followed by the LA + HM treatment, increasing the intensity of the rose flavor characteristics of the ‘Jumeigui’ grape. Therefore, the combination of MeJA and ABA at different concentrations had distinct effects on fruit quality and appropriate combinations can be selected according to the specific needs for the targeted metabolites.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"48 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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