{"title":"The critical role of CmCIPK1-CmRbohD1/D2 complexes in generating H2O2 signals for enhancing salt tolerance in pumpkins","authors":"Lanxing Wei, Dandi Xu, Lijian Zhou, Hui Chen, Z. Peng, Guoyu Chen, Lihui Wang, H. Cao, Yuquan Peng, Shouyu Geng, Zhilong Bie","doi":"10.1016/j.hpj.2023.12.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.12.004","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140767983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cold tolerance is one of the important traits for grapevine, especially in regions with extremely low temperatures in winter. is wild species in the genus with excellent cold hardiness compared with . However, metabolites that contribute to the cold tolerance of remain unknown. Here, the metabolomics of buds from ‘Zuoshan-1’ during cold acclimation (CA) were identified, and cold-sensitive cultivar ( ‘Jingzaojing’) was used as the control. The buds were collected in October, November, and December in 2016 and 2018. The cold hardiness of the buds increased during CA in the two grapevines. However, browning was observed only in buds at temperature below −10 °C. Among detected metabolites from buds, 443 metabolites were overlapped between two years. Forty-four and thirty differentially accumulated metabolites (DAMs) were identified in and , respectively. Ten DAMs including monoacylglycerol (MAG, 18: 2) isomer 1, trehalose 6-phosphate, and -glucose showed identical variations in the two grapevines, indicating conserved CA responses within the genus. Eighteen DAMs exhibited higher accumulation in than in . Maltotetraose, -glucoronic acid, -aspartic acid, azelaic acid, and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants. Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L-aspartic acid and 1% proanthocyanidins. Potential contributions of other DAMs found in such as Cyanidin 3-O-glucoside need to be further elucidated. Thus, eighteen metabolites accumulated in can be used for practical application in improvement of cold resistance in grapevine. Our findings provide new insights into understanding the cold hardiness of .
耐寒性是葡萄的重要性状之一，尤其是在冬季气温极低的地区。 与......相比，......是葡萄属中耐寒性极佳的野生品种。 然而，导致葡萄耐寒性的代谢物仍然未知。本文鉴定了 "左山-1 "在冷适应（CA）过程中芽的代谢组学，并以对冷敏感的栽培品种（"京早"）为对照。花蕾采集时间为2016年和2018年的10月、11月和12月。在 CA 期间，两种葡萄树花蕾的耐寒性都有所提高。然而，只有在温度低于-10 °C时，芽才会出现褐变。在从花蕾中检测到的代谢物中，有 443 种代谢物在两年中重叠。分别有 44 种和 30 种差异积累代谢物（DAMs）在Ⅴ年和Ⅵ年被发现。包括单酰基甘油（MAG，18: 2）异构体 1、6-磷酸三卤糖和-葡萄糖在内的 10 种 DAMs 在两种葡萄树中表现出相同的变化，这表明在葡萄属中 CA 反应是一致的。有 18 种 DAM 在......中的积累高于在......中的积累。据报道，麦芽四糖、-葡萄糖酸、-天冬氨酸、壬二酸和 4-羟基苯甲酸会在其他植物的 CA 过程中积累。外源 5 mmol L-天冬氨酸和 1%原花青素可增强葡萄叶片的耐寒性。其他 DAMs（如花青素 3-O-葡萄糖苷）的潜在贡献有待进一步阐明。因此，葡萄中积累的 18 种代谢物可用于提高葡萄的抗寒性。我们的发现为了解......葡萄的耐寒性提供了新的视角。
{"title":"Identification of potential metabolites responsible for cold tolerance in buds of Vitis amurensis","authors":"Qingyun Li, Huimin Zhou, Fengmei Chai, Zemin Wang, Linchuan Fang, Wei Duan, Peige Fan, Zhenchang Liang, Shaohua Li, Qingfeng Wang, Haiping Xin","doi":"10.1016/j.hpj.2023.07.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.07.009","url":null,"abstract":"Cold tolerance is one of the important traits for grapevine, especially in regions with extremely low temperatures in winter. is wild species in the genus with excellent cold hardiness compared with . However, metabolites that contribute to the cold tolerance of remain unknown. Here, the metabolomics of buds from ‘Zuoshan-1’ during cold acclimation (CA) were identified, and cold-sensitive cultivar ( ‘Jingzaojing’) was used as the control. The buds were collected in October, November, and December in 2016 and 2018. The cold hardiness of the buds increased during CA in the two grapevines. However, browning was observed only in buds at temperature below −10 °C. Among detected metabolites from buds, 443 metabolites were overlapped between two years. Forty-four and thirty differentially accumulated metabolites (DAMs) were identified in and , respectively. Ten DAMs including monoacylglycerol (MAG, 18: 2) isomer 1, trehalose 6-phosphate, and -glucose showed identical variations in the two grapevines, indicating conserved CA responses within the genus. Eighteen DAMs exhibited higher accumulation in than in . Maltotetraose, -glucoronic acid, -aspartic acid, azelaic acid, and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants. Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L-aspartic acid and 1% proanthocyanidins. Potential contributions of other DAMs found in such as Cyanidin 3-O-glucoside need to be further elucidated. Thus, eighteen metabolites accumulated in can be used for practical application in improvement of cold resistance in grapevine. Our findings provide new insights into understanding the cold hardiness of .","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140340777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-17DOI: 10.1016/j.hpj.2023.06.008
Hawar Sleman Halshoy, Sadik Kasim Sadik
Pepper is an important agricultural crop because of the nutritional value of the fruit and its economic importance. Various techniques have been practiced to enhance both the productivity and nutritional value of pepper. Therefore, this study was conducted to determine the impact of different training methods and biostimulants applications on the growth, yield, and chemical composition of sweet pepper ( L.) plants under greenhouse conditions. For the training method, unpruned plants were compared with one stem and two stem plants, and for biostimulant applications, control plants were compared with the Disper Root (DR) and Disper Vital (DV). Unpruned plants had the fruit number (33.98), fruit weight (2.18 kg . plant-1), total marketable yield (109 ton . ha-1), and TSS (7.21 %) when considering plants that have either one or two stems for comparison. However, two stem plants had the highest fruit setting (62.41 %), carotenoid (1.37 mg . 100g-1 FW), fruit chlorophyll (35.68 mg . 100g-1 FW), and vitamin C (136.56 mg . 100g FW), the contents were compared to other treatments. DR application resulted to increase in total sugar, carotenoid, vitamin C, and TSS contents significantly compared to both the control and DV application. While, DV application increased fruit setting, plant fruit number, plant fruit weight, and total marketable yield. In addition, integrating one stem plant with the DR application improved fiber, vitamin C, and TSS contents significantly, and two stem plants and the DV application improved fruit setting and carotenoid content. Thus, training methods integrated with biostimulant applications could be considered for developing agricultural practices to obtain commercial yield and improve the chemical composition of sweet pepper, as unpruned plants without biostimulant applications gave a negative impact.
辣椒是一种重要的农作物，因为其果实具有营养价值和重要的经济价值。为了提高辣椒的产量和营养价值，人们采用了各种技术。因此，本研究旨在确定不同的培植方法和生物刺激剂的应用对温室条件下甜椒植株的生长、产量和化学成分的影响。在栽培方法方面，未修剪植株与单茎植株和双茎植株进行了比较；在施用生物刺激剂方面，对照植株与Disper Root（DR）和Disper Vital（DV）进行了比较。在对单茎或双茎植物进行比较时，未修剪植物的果实数量（33.98 个）、果实重量（2.18 千克.株-1）、可销售总产量（109 吨.公顷-1）和总悬浮固体含量（7.21 %）最高。然而，与其他处理相比，双茎植株的坐果率（62.41 %）、类胡萝卜素（1.37 毫克.100 克-1 水分）、果实叶绿素（35.68 毫克.100 克-1 水分）和维生素 C（136.56 毫克.100 克-1 水分）含量最高。与对照和施用 DV 相比，施用 DR 会显著增加总糖、类胡萝卜素、维生素 C 和 TSS 的含量。而施用 DV 则提高了坐果率、植株果实数、植株果实重量和可销售总产量。此外，在施用 DR 的同时种植一株茎叶，纤维、维生素 C 和 TSS 含量都有明显提高；在施用 DV 的同时种植两株茎叶，坐果率和类胡萝卜素含量都有提高。因此，在发展农业实践时，可以考虑采用与施用生物刺激剂相结合的培训方法，以获得甜椒的商业产量并改善其化学成分，因为不施用生物刺激剂的未修剪植株会产生负面影响。
{"title":"Impact of training methods and biostimulant applications on sweet pepper (Capsicum annuum) yield and nutritional values: under greenhouse condition","authors":"Hawar Sleman Halshoy, Sadik Kasim Sadik","doi":"10.1016/j.hpj.2023.06.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.06.008","url":null,"abstract":"Pepper is an important agricultural crop because of the nutritional value of the fruit and its economic importance. Various techniques have been practiced to enhance both the productivity and nutritional value of pepper. Therefore, this study was conducted to determine the impact of different training methods and biostimulants applications on the growth, yield, and chemical composition of sweet pepper ( L.) plants under greenhouse conditions. For the training method, unpruned plants were compared with one stem and two stem plants, and for biostimulant applications, control plants were compared with the Disper Root (DR) and Disper Vital (DV). Unpruned plants had the fruit number (33.98), fruit weight (2.18 kg . plant-1), total marketable yield (109 ton . ha-1), and TSS (7.21 %) when considering plants that have either one or two stems for comparison. However, two stem plants had the highest fruit setting (62.41 %), carotenoid (1.37 mg . 100g-1 FW), fruit chlorophyll (35.68 mg . 100g-1 FW), and vitamin C (136.56 mg . 100g FW), the contents were compared to other treatments. DR application resulted to increase in total sugar, carotenoid, vitamin C, and TSS contents significantly compared to both the control and DV application. While, DV application increased fruit setting, plant fruit number, plant fruit weight, and total marketable yield. In addition, integrating one stem plant with the DR application improved fiber, vitamin C, and TSS contents significantly, and two stem plants and the DV application improved fruit setting and carotenoid content. Thus, training methods integrated with biostimulant applications could be considered for developing agricultural practices to obtain commercial yield and improve the chemical composition of sweet pepper, as unpruned plants without biostimulant applications gave a negative impact.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140162200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation, epigenetic factor, and post-translational modification. Previously, we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation. However, whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown. In this study, we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence (DAP-Seq) approach. Electrophoresis mobility shift assay (EMSA) and dual-luciferase reporter assay (DLR) demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes , and . Further analyses of yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and Luciferase complementation imaging (LCI) assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1, and this interaction weakened the MaERF11-mediated transcriptional repression capacity. Collectively, our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.
{"title":"Interaction of MaERF11 with an E3 ubiquitin ligase MaRFA1 is involved in regulation of banana starch degradation during postharvest ripening","authors":"Mengge Jiang, Yingying Yang, Wei Wei, Chaojie Wu, Wei Shan, Jianfei Kuang, Jianye Chen, Shouxing Wei, Wangjin Lu","doi":"10.1016/j.hpj.2023.09.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.09.006","url":null,"abstract":"Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation, epigenetic factor, and post-translational modification. Previously, we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation. However, whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown. In this study, we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence (DAP-Seq) approach. Electrophoresis mobility shift assay (EMSA) and dual-luciferase reporter assay (DLR) demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes , and . Further analyses of yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and Luciferase complementation imaging (LCI) assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1, and this interaction weakened the MaERF11-mediated transcriptional repression capacity. Collectively, our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140162196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-16DOI: 10.1016/j.hpj.2023.08.002
Jorge Alemán-Báez, Jose Fernando Acevedo-Zamora, Johan Bucher, Chengcheng Cai, Roeland E. Voorrips, Guusje Bonnema
Cabbage ( var. ) is an economically important vegetable crop grown for its leafy head. Cabbage plants’ vegetative development goes from seedling to rosette, folding and heading stages. Leaves that form the rosette are large and mostly flat, acting as the major photosynthetic organs. In the following developmental stages, the plants produce leaves that curve inward, overlapping the shoot apex to produce the leafy head. These leaves are shielded from the light and act as sink organs. We investigated the role of miRNA-regulated genes in the transition from the rosette to the heading stage. We first phenotyped leaf formation and -expansion for two contrasting (round and pointed) heading cabbage morphotypes to define the rosette and heading developmental stages. This allowed us to collect tissue from young expanding leaves that would develop into, respectively, rosette and heading leaves for miRNA and gene expression analyses. Young leaf tissue of two time points of a non-heading collard green ( var. ) morphotype was used as a control to distinguish the transcripts (miRNAs/genes) that regulate plant age from those defining the transition from rosette to heading leaves in cabbages. For both round and pointed cabbages, we compared the miRNA and mRNA abundances between rosette and heading leaves to identify differentially expressed miRNAs (DEMs) and –genes (DEGs). After correcting for miRNAs and genes related to plant age, we identified 33 DEMs and 1,998 DEGs with roles in the transition from rosette to heading stage. We predicted the target genes of these 33 DEMs and focused on the subset that were DEGs between rosette and heading stage leaves to construct miRNA-target gene interaction networks. Our main finding is a role for miR396b-5p targeting two orthologues of () and - () in pointed cabbage head formation.
{"title":"Expression changes of miRNA-regulated genes associated with the formation of the leafy head in cabbage (Brassica oleracea var. capitata).","authors":"Jorge Alemán-Báez, Jose Fernando Acevedo-Zamora, Johan Bucher, Chengcheng Cai, Roeland E. Voorrips, Guusje Bonnema","doi":"10.1016/j.hpj.2023.08.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.08.002","url":null,"abstract":"Cabbage ( var. ) is an economically important vegetable crop grown for its leafy head. Cabbage plants’ vegetative development goes from seedling to rosette, folding and heading stages. Leaves that form the rosette are large and mostly flat, acting as the major photosynthetic organs. In the following developmental stages, the plants produce leaves that curve inward, overlapping the shoot apex to produce the leafy head. These leaves are shielded from the light and act as sink organs. We investigated the role of miRNA-regulated genes in the transition from the rosette to the heading stage. We first phenotyped leaf formation and -expansion for two contrasting (round and pointed) heading cabbage morphotypes to define the rosette and heading developmental stages. This allowed us to collect tissue from young expanding leaves that would develop into, respectively, rosette and heading leaves for miRNA and gene expression analyses. Young leaf tissue of two time points of a non-heading collard green ( var. ) morphotype was used as a control to distinguish the transcripts (miRNAs/genes) that regulate plant age from those defining the transition from rosette to heading leaves in cabbages. For both round and pointed cabbages, we compared the miRNA and mRNA abundances between rosette and heading leaves to identify differentially expressed miRNAs (DEMs) and –genes (DEGs). After correcting for miRNAs and genes related to plant age, we identified 33 DEMs and 1,998 DEGs with roles in the transition from rosette to heading stage. We predicted the target genes of these 33 DEMs and focused on the subset that were DEGs between rosette and heading stage leaves to construct miRNA-target gene interaction networks. Our main finding is a role for miR396b-5p targeting two orthologues of () and - () in pointed cabbage head formation.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140162209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-09DOI: 10.1016/j.hpj.2023.11.006
Ho-Young Jeong, Yoonseo Lim, Myeong-Gyun Seo, Soon Ju Park, Chanhui Lee, Young-Joon Park, Choon-Tak Kwon
Senescence, a crucial developmental process in the life cycle of plants, involves programmed destruction of cellular components of leaves. The onset of senescence is synchronized with other developmental processes for successful reproduction since senescence eventually leads to cell death. Arabinosyltransferase FASCIATED AND BRANCHED 2 (FAB2) is known to control meristem proliferation. Here, we show that FAB2 can inhibit premature leaf senescence in tomato plants. Both chemically mutagenized and CRISPR-generated mutants exhibited excessively accelerated senescence, which resulted in sterility. Transcriptome analysis revealed that extended leaf longevity by suppressing transcription of genes highly expressed in mature leaves. Transcription of was increased in younger leaves, potentially inhibiting premature leaf senescence. The precocious senescence of mutants was in contrast to () mutants, which carried mutations in a hydroxyproline O-arabinosyltransferase gene, leading to meristem overproliferation. Our observations indicate that complex genetic hierarchy in the cascade of tomato arabinosyltransferases could control different aspects of developmental processes such as stem cell proliferation and senescence.
衰老是植物生命周期中的一个关键发育过程，涉及叶片细胞成分的程序性破坏。由于衰老最终会导致细胞死亡，因此衰老的开始要与其他发育过程同步，这样才能成功繁殖。已知阿拉伯糖基转移酶 FASCIATED AND BRANCHED 2（FAB2）能控制分生组织的增殖。在这里，我们发现 FAB2 可以抑制番茄植株叶片的过早衰老。化学诱变和 CRISPR 产生的突变体都表现出过度加速衰老，从而导致不育。转录组分析揭示，通过抑制成熟叶片中高表达基因的转录，延长了叶片的寿命。年轻叶片中的基因转录增加，可能抑制了叶片的过早衰老。突变体的过早衰老与（）突变体形成鲜明对比，后者携带羟脯氨酸 O-阿拉伯糖基转移酶基因突变，导致分生组织过度增殖。我们的观察结果表明，番茄阿拉伯糖基转移酶级联中复杂的遗传层次可控制干细胞增殖和衰老等发育过程的不同方面。
{"title":"Tomato arabinosyltransferase prevents precocious senescence","authors":"Ho-Young Jeong, Yoonseo Lim, Myeong-Gyun Seo, Soon Ju Park, Chanhui Lee, Young-Joon Park, Choon-Tak Kwon","doi":"10.1016/j.hpj.2023.11.006","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.11.006","url":null,"abstract":"Senescence, a crucial developmental process in the life cycle of plants, involves programmed destruction of cellular components of leaves. The onset of senescence is synchronized with other developmental processes for successful reproduction since senescence eventually leads to cell death. Arabinosyltransferase FASCIATED AND BRANCHED 2 (FAB2) is known to control meristem proliferation. Here, we show that FAB2 can inhibit premature leaf senescence in tomato plants. Both chemically mutagenized and CRISPR-generated mutants exhibited excessively accelerated senescence, which resulted in sterility. Transcriptome analysis revealed that extended leaf longevity by suppressing transcription of genes highly expressed in mature leaves. Transcription of was increased in younger leaves, potentially inhibiting premature leaf senescence. The precocious senescence of mutants was in contrast to () mutants, which carried mutations in a hydroxyproline O-arabinosyltransferase gene, leading to meristem overproliferation. Our observations indicate that complex genetic hierarchy in the cascade of tomato arabinosyltransferases could control different aspects of developmental processes such as stem cell proliferation and senescence.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-08DOI: 10.1016/j.hpj.2024.01.004
Mughair Abdul Aziz, Khaled Masmoudi
Advancements in molecular approaches have been utilized to breed crops with a wide range of economically valuable traits to develop superior cultivars. This review provides a concise overview of modern breakthroughs in molecular plant production. Genotyping and high-throughput phenotyping methods for predictive plant breeding are briefly discussed. In this study, we explore contemporary molecular breeding techniques for producing desirable crop varieties. These techniques include cisgenesis, clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) gene editing, haploid induction, and de novo domestication. We examine the speed breeding approach—a strategy for cultivating plants under controlled conditions. We further highlight the significance of modern breeding technologies in efficiently utilizing agricultural resources for crop production in urban areas. The deciphering of crop genomes has led to the development of extensive DNA markers, quantitative trait loci (QTLs), and pangenomes associated with various desirable crop traits. This shift to the genotypic selection of crops considerably expedites the plant breeding process. Based on the plant population used, the connection between genotypic and phenotypic data provides several genetic elements, including genes, markers, and alleles that can be used in genomic breeding and gene editing. The integration of speed breeding with genomic-assisted breeding and cutting-edge genome editing tools has made it feasible to rapidly manipulate and generate multiple crop cycles and accelerate the plant breeding process. Breakthroughs in molecular techniques have led to substantial improvements in modern breeding methods.
分子方法的进步已被用于培育具有多种经济价值性状的作物，以培育优良品种。本综述简要概述了分子植物育种的现代突破。简要讨论了用于预测性植物育种的基因分型和高通量表型方法。在本研究中，我们探讨了培育理想作物品种的现代分子育种技术。这些技术包括顺式育种、聚类规则间隔短回文重复（CRISPR/Cas9）基因编辑、单倍体诱导和从头驯化。我们研究了快速育种方法--一种在受控条件下培育植物的策略。我们进一步强调了现代育种技术在有效利用农业资源促进城市地区作物生产方面的重要意义。作物基因组的解密导致了与各种理想作物性状相关的大量 DNA 标记、数量性状位点（QTL）和泛基因组的开发。向作物基因型选择的转变大大加快了植物育种进程。根据所使用的植物群体，基因型和表型数据之间的联系提供了若干遗传要素，包括基因、标记和等位基因，可用于基因组育种和基因编辑。速度育种与基因组辅助育种和尖端基因组编辑工具的结合，使得快速操作和生成多个作物周期以及加速植物育种过程变得可行。分子技术的突破极大地改进了现代育种方法。
{"title":"Molecular Breakthroughs in Modern Plant Breeding Techniques","authors":"Mughair Abdul Aziz, Khaled Masmoudi","doi":"10.1016/j.hpj.2024.01.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.01.004","url":null,"abstract":"Advancements in molecular approaches have been utilized to breed crops with a wide range of economically valuable traits to develop superior cultivars. This review provides a concise overview of modern breakthroughs in molecular plant production. Genotyping and high-throughput phenotyping methods for predictive plant breeding are briefly discussed. In this study, we explore contemporary molecular breeding techniques for producing desirable crop varieties. These techniques include cisgenesis, clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) gene editing, haploid induction, and de novo domestication. We examine the speed breeding approach—a strategy for cultivating plants under controlled conditions. We further highlight the significance of modern breeding technologies in efficiently utilizing agricultural resources for crop production in urban areas. The deciphering of crop genomes has led to the development of extensive DNA markers, quantitative trait loci (QTLs), and pangenomes associated with various desirable crop traits. This shift to the genotypic selection of crops considerably expedites the plant breeding process. Based on the plant population used, the connection between genotypic and phenotypic data provides several genetic elements, including genes, markers, and alleles that can be used in genomic breeding and gene editing. The integration of speed breeding with genomic-assisted breeding and cutting-edge genome editing tools has made it feasible to rapidly manipulate and generate multiple crop cycles and accelerate the plant breeding process. Breakthroughs in molecular techniques have led to substantial improvements in modern breeding methods.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In grapevine, previous studies have suggested that, gibberellin (GA) inhibits bud break before dormancy release while enhanced after dormancy release; the capacity of GA accumulation shows a trend of first inhibition and then upregulation. However, the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release. In this study, we further validated the effect of GA and hydrogen cyanamide (HC) on bud break of ‘Red globe’ grape, confirmed inhibition and promotion effect, respectively. Restricted GA biosynthetic genes’ expression and enhanced GA catabolic gene’s expression were observed in the early stage after HC treatment, while opposite expression trend showed in the late stage. , a MADS-box transcription factor gene, was downregulated in the late stage, which might play an important role in regulating GA metabolism genes’ expression. It was shown that, VvSVP1 could bind to the promoter regions of GA biosynthetic gene and catabolic gene , negatively and positively regulated the corresponding genes’ expression, respectively; the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed , while the ratio of GAs related to GA2ox were significantly increased. Taken together, VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC. Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.
以往的研究表明，赤霉素（GA）在葡萄休眠解除前对花芽分化有抑制作用，而在休眠解除后对花芽分化有促进作用；GA的积累能力呈现先抑后扬的趋势。然而，休眠解除过程中 GA 代谢基因表达的调控机制尚不清楚。本研究进一步验证了GA和氢氰酸酰胺（HC）对'红地球'葡萄花芽分化的影响，分别证实了抑制和促进作用。HC处理后，早期GA生物合成基因表达受限，GA分解代谢基因表达增强，而后期则表现出相反的表达趋势。在后期，MADS-box 转录因子基因Ⅴ表达下调，这可能在调控 GA 代谢基因的表达中起着重要作用。研究表明，VvSVP1能与GA生物合成基因和分解代谢基因的启动子区域结合，分别对相应基因的表达产生负调控和正调控作用；在过表达VvSVP1的葡萄胼胝体中，与GA20ox相关的GA含量显著降低，而与GA2ox相关的GA比例显著增加。综上所述，VvSVP1可在HC诱导的休眠芽断裂前通过调控GA代谢基因的表达来调节内源GA水平。我们的研究结果可为多年生木本果树花芽休眠调控的研究提供一些新的理论依据。
{"title":"VvSVP1 negatively regulates gibberellin accumulation before the dormant bud break of grapevine triggered by hydrogen cyanamide","authors":"Jingyi Li, Pinqi Sun, Anni Chen, Jilong Xu, Peiyong Xin, Jinfang Chu, Chuanlin Zheng","doi":"10.1016/j.hpj.2023.12.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.12.002","url":null,"abstract":"In grapevine, previous studies have suggested that, gibberellin (GA) inhibits bud break before dormancy release while enhanced after dormancy release; the capacity of GA accumulation shows a trend of first inhibition and then upregulation. However, the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release. In this study, we further validated the effect of GA and hydrogen cyanamide (HC) on bud break of ‘Red globe’ grape, confirmed inhibition and promotion effect, respectively. Restricted GA biosynthetic genes’ expression and enhanced GA catabolic gene’s expression were observed in the early stage after HC treatment, while opposite expression trend showed in the late stage. , a MADS-box transcription factor gene, was downregulated in the late stage, which might play an important role in regulating GA metabolism genes’ expression. It was shown that, VvSVP1 could bind to the promoter regions of GA biosynthetic gene and catabolic gene , negatively and positively regulated the corresponding genes’ expression, respectively; the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed , while the ratio of GAs related to GA2ox were significantly increased. Taken together, VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC. Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N-methyladenosine (mA) RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic organisms. Despite its significance, a comprehensive analysis of mA-related genes in non-model plants, such as kiwifruit, is lacking. Here, we identified 36 mA-related genes in the kiwifruit genome according to homology and phylogenetic inference. We performed bioinformatics and evolutionary analyses of the writer, eraser, and reader families of mA modification. Reanalysis of public RNA-seq data collected from samples under various biotic and abiotic stresses indicated that most mA-related genes were remarkably expressed under different conditions. Through construction of gene co-expression networks, we found significant correlations between several mA-related genes and transcription factors (TFs) as well as receptor-like genes during the development and ripening of kiwifruit. Furthermore, we performed ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory mechanisms of mA-related genes. We identified 10 common open chromatin regions that were present in at least two tissues, and these regions might serve as potential binding sites for MADS protein, C2H2 protein, and other predicted TFs. Our study offers comprehensive insights into the gene family of mA-related components in kiwifruit, which will lay foundation for exploring mechanisms of post-transcriptional regulation involved in development and adaptation of kiwifruit.
N-甲基腺苷（mA）RNA修饰是一种调节真核生物中RNA命运的保守机制。尽管其意义重大，但目前还缺乏对猕猴桃等非模式植物中 mA 相关基因的全面分析。在此，我们根据同源性和系统发育推断在猕猴桃基因组中鉴定了 36 个 mA 相关基因。我们对mA修饰的书写器、橡皮擦和阅读器家族进行了生物信息学和进化分析。对从各种生物和非生物胁迫下的样本中收集的公开 RNA-seq 数据进行的再分析表明，大多数 mA 相关基因在不同条件下都有显著表达。通过构建基因共表达网络，我们发现在猕猴桃的发育和成熟过程中，多个 mA 相关基因与转录因子（TFs）以及受体类基因之间存在显著的相关性。此外，我们还对不同的猕猴桃组织进行了 ATAC-seq 分析，以研究 mA 相关基因的调控机制。我们发现了 10 个共同的开放染色质区域，它们至少存在于两个组织中，这些区域可能是 MADS 蛋白、C2H2 蛋白和其他预测的 TFs 的潜在结合位点。我们的研究全面揭示了猕猴桃中 mA 相关成分的基因家族，这将为探索猕猴桃发育和适应过程中的转录后调控机制奠定基础。
{"title":"Identification and characterization of genes related to m6A modification in kiwifruit using RNA-seq and ATAC-seq","authors":"Xiaoli Hu, Tong Li, Changbin Xu, Yanna Xu, Congjun You, Xinyi Li, Jinli Gong, Xiaolong Li, Xuepeng Sun","doi":"10.1016/j.hpj.2024.02.001","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.02.001","url":null,"abstract":"N-methyladenosine (mA) RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic organisms. Despite its significance, a comprehensive analysis of mA-related genes in non-model plants, such as kiwifruit, is lacking. Here, we identified 36 mA-related genes in the kiwifruit genome according to homology and phylogenetic inference. We performed bioinformatics and evolutionary analyses of the writer, eraser, and reader families of mA modification. Reanalysis of public RNA-seq data collected from samples under various biotic and abiotic stresses indicated that most mA-related genes were remarkably expressed under different conditions. Through construction of gene co-expression networks, we found significant correlations between several mA-related genes and transcription factors (TFs) as well as receptor-like genes during the development and ripening of kiwifruit. Furthermore, we performed ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory mechanisms of mA-related genes. We identified 10 common open chromatin regions that were present in at least two tissues, and these regions might serve as potential binding sites for MADS protein, C2H2 protein, and other predicted TFs. Our study offers comprehensive insights into the gene family of mA-related components in kiwifruit, which will lay foundation for exploring mechanisms of post-transcriptional regulation involved in development and adaptation of kiwifruit.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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