Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.15503
Berlian Beatrix Rarome, Nur Aisah, R. A. Setyoningrum, N. Mertaniasih
The diagnosis of childhood tuberculosis remains a challenge worldwide. The GeneXpert MTB/RIF test, a rapid Mycobacteria tuberculosis diagnostic tool, was recommended for use in children. No pediatric studies of GeneXpert MTB/RIF assessing pulmonary tuberculosis within a hospital setting has been done in Indonesia. We evaluated the performance of the GeneXpert MTB/RIF test compared with sputum culture on Lowenstein-Jensen (LJ) for the diagnosis of childhood pulmonary tuberculosis. This study was conducted in pediatric respirology inpatient and outpatient Dr. Soetomo Hospital, a tertiary care facility in Surabaya between June and August 2015 with a cross-sectional design. We consecutively enrolled 27 children aged 3 months to 14 years who had history of close contact with adult tuberculosis patients and showed symptoms of pulmonary tuberculosis. Sputum collection was performed by induced sputum and three examination methods were performed (microscopic, GeneXpert MTB/RIF and sputum culture) simultaneously followed by a drug sensitivity test for specimens detected with MTB growth. The GeneXpert MTB/RIF test had a sensitivity of 100% (95% CI 100-100) and a specificity of 95% (95% CI 85-100). The positive predictive value for diagnosing pulmonary TB was 89% (95% CI 68-100), the negative predictive value was 100% (95% CI 100-100) and positive likelihood ratio was 20 (95% CI 2.82-128). The GeneXpert MTB/RIF test on one sputum sample rapidly and correctly identified all children with culture-confirmed pulmonary tuberculosis with high specificity. Similar results were obtained between GeneXpert MTB/RIF and sputum culture based on age groups and clinical manifestations. Rifampicin resistance were both detected in GeneXpert MTB/RIF and MTB sensitivity test
儿童结核病的诊断在全世界仍然是一项挑战。推荐在儿童中使用GeneXpert MTB/RIF检测，这是一种结核分枝杆菌快速诊断工具。印度尼西亚还没有开展GeneXpert MTB/RIF在医院环境中评估肺结核的儿科研究。我们评估了GeneXpert MTB/RIF试验与洛温斯坦-詹森(LJ)痰培养诊断儿童肺结核的性能。本研究于2015年6月至8月在泗水三级医疗机构Dr. Soetomo医院的儿科呼吸内科住院和门诊患者中进行，采用横断面设计。我们连续招募了27名年龄在3个月至14岁之间的儿童，这些儿童都曾与成年结核病患者有过密切接触，并表现出肺结核症状。采用诱导痰法采集痰液，同时采用显微镜、GeneXpert MTB/RIF和痰培养三种检查方法，对检测到结核分枝杆菌生长的标本进行药敏试验。GeneXpert MTB/RIF检测的敏感性为100% (95% CI 100-100)，特异性为95% (95% CI 85-100)。诊断肺结核的阳性预测值为89% (95% CI 68 ~ 100)，阴性预测值为100% (95% CI 100 ~ 100)，阳性似然比为20 (95% CI 2.82 ~ 128)。对一份痰样本进行的GeneXpert MTB/RIF检测具有高特异性，能够快速、正确地识别出所有经培养证实的肺结核儿童。基于年龄组和临床表现，GeneXpert MTB/RIF与痰培养结果相似。GeneXpert MTB/RIF和MTB敏感性试验均检测到利福平耐药
{"title":"GeneXpert MTB/RIF and Mycobacterium tuberculosis Sputum Culture in Establishing the Diagnosis of Pulmonary Tuberculosis and Rifampicin Resistance in Suspected Childhood Pulmonary Tuberculosis in Soetomo Hospital","authors":"Berlian Beatrix Rarome, Nur Aisah, R. A. Setyoningrum, N. Mertaniasih","doi":"10.20473/ijtid.v8i3.15503","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.15503","url":null,"abstract":"The diagnosis of childhood tuberculosis remains a challenge worldwide. The GeneXpert MTB/RIF test, a rapid Mycobacteria tuberculosis diagnostic tool, was recommended for use in children. No pediatric studies of GeneXpert MTB/RIF assessing pulmonary tuberculosis within a hospital setting has been done in Indonesia. We evaluated the performance of the GeneXpert MTB/RIF test compared with sputum culture on Lowenstein-Jensen (LJ) for the diagnosis of childhood pulmonary tuberculosis. This study was conducted in pediatric respirology inpatient and outpatient Dr. Soetomo Hospital, a tertiary care facility in Surabaya between June and August 2015 with a cross-sectional design. We consecutively enrolled 27 children aged 3 months to 14 years who had history of close contact with adult tuberculosis patients and showed symptoms of pulmonary tuberculosis. Sputum collection was performed by induced sputum and three examination methods were performed (microscopic, GeneXpert MTB/RIF and sputum culture) simultaneously followed by a drug sensitivity test for specimens detected with MTB growth. The GeneXpert MTB/RIF test had a sensitivity of 100% (95% CI 100-100) and a specificity of 95% (95% CI 85-100). The positive predictive value for diagnosing pulmonary TB was 89% (95% CI 68-100), the negative predictive value was 100% (95% CI 100-100) and positive likelihood ratio was 20 (95% CI 2.82-128). The GeneXpert MTB/RIF test on one sputum sample rapidly and correctly identified all children with culture-confirmed pulmonary tuberculosis with high specificity. Similar results were obtained between GeneXpert MTB/RIF and sputum culture based on age groups and clinical manifestations. Rifampicin resistance were both detected in GeneXpert MTB/RIF and MTB sensitivity test","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48163330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.12226
Edward Muliawan Putera, W. Widodo, N. Mardiana
Complications such as anemia and its clinical consequences arise as chronic kidney diseases progress,. One renal anemia pathophysiology is a disruption of iron metabolism, regulated by the main iron exporter hormone, hepcidin. Chronic kidney disease patients were constantly in an inflammatory state, represented by an increased in C-reactive protein. This inflammatory state would facilitate the liver to secrete hepcidin, which would subsequently follow a decrease of iron circulation, thus resulting in functional iron deficiency. Both acute phase reactants which used thoroughly as markers in tropical and infectious diseases, had their own roles in chronic kidney disease. The correlation of c-reactive protein and hepcidin in chronic kidney disease patients was still controversial. To analyse the relationship between c-reactive protein and hepcidin in non-dialysis chronic kidney disease patients. We conducted an observational cross-sectional study with 40 non-dialysis chronic kidney disease patients who met the inclusion and exclusion criteria. Patients were enrolled with consecutive sampling and were examined for serum c-reactive protein and hepcidin levels.A total of forty subjects (67.5% male with mean age of 50.23 ± 1.04 years) were eligible for enrolment in this study. The most comorbid factor was hypertension (62.5%). The common stage for chronic kidney disease was stage 3 (40%). The mean hemoglobin value was 10.74 ± 0.36 g/dL, mean blood urea nitrogen was 39.98 ± 29.59 mg/dL, and serum creatinine of 4.12 ± 3.39 mg/dL. Mean serum c-reactive protein levels were 3.52 ± 5.13 mg/l. Mean hepcidin level were 94,03 ± 95,39 ng/ml. Serum C-reactive protein levels correlated positively (r=0.487) and significantly (p-value=0.001) with serum hepcidin value. C-reactive protein and hepcidin was significantly correlated in non-dialysis chronic kidney disease patients.
{"title":"C-reactive Protein and Hepcidin in Non-Dialysis Chronic Kidney Disease","authors":"Edward Muliawan Putera, W. Widodo, N. Mardiana","doi":"10.20473/ijtid.v8i3.12226","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.12226","url":null,"abstract":"Complications such as anemia and its clinical consequences arise as chronic kidney diseases progress,. One renal anemia pathophysiology is a disruption of iron metabolism, regulated by the main iron exporter hormone, hepcidin. Chronic kidney disease patients were constantly in an inflammatory state, represented by an increased in C-reactive protein. This inflammatory state would facilitate the liver to secrete hepcidin, which would subsequently follow a decrease of iron circulation, thus resulting in functional iron deficiency. Both acute phase reactants which used thoroughly as markers in tropical and infectious diseases, had their own roles in chronic kidney disease. The correlation of c-reactive protein and hepcidin in chronic kidney disease patients was still controversial. To analyse the relationship between c-reactive protein and hepcidin in non-dialysis chronic kidney disease patients. We conducted an observational cross-sectional study with 40 non-dialysis chronic kidney disease patients who met the inclusion and exclusion criteria. Patients were enrolled with consecutive sampling and were examined for serum c-reactive protein and hepcidin levels.A total of forty subjects (67.5% male with mean age of 50.23 ± 1.04 years) were eligible for enrolment in this study. The most comorbid factor was hypertension (62.5%). The common stage for chronic kidney disease was stage 3 (40%). The mean hemoglobin value was 10.74 ± 0.36 g/dL, mean blood urea nitrogen was 39.98 ± 29.59 mg/dL, and serum creatinine of 4.12 ± 3.39 mg/dL. Mean serum c-reactive protein levels were 3.52 ± 5.13 mg/l. Mean hepcidin level were 94,03 ± 95,39 ng/ml. Serum C-reactive protein levels correlated positively (r=0.487) and significantly (p-value=0.001) with serum hepcidin value. C-reactive protein and hepcidin was significantly correlated in non-dialysis chronic kidney disease patients.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47528152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.12570
Benaya Yamin Onesiforus, Indra Elisabet Lalangpuling, M. A. Wijayanti, E. H. Murhandarwati
Malnutrition can reduce immune response particularly in cytokine (IL-4, IL-5, IL-10) production and immune effector (eosinophil, IgE, and mast cell), thus increasing the probability of intestinal nematode infection. Through this study, intestinal nematode infections occurred among children under five years, at different nutrition status, in Kokar Public Health center, Alor Regency, East Nusa Tenggara was captured. Hookworm and Strongyloides stercoralis were studied as both of them have devastating impacts compare to other helminthes compare to other helminths. This study is a cross- sectional study with a quote sampling technique. As many as 238 children, aged 12-59 months living in Kokar’s Public Health Center area, Alor regency were recruited in this study i.e. 7.7% severely underweight, 19.2% underweight, 70.5% normal and 2.6% overweight. Data were collected in August - October 2016. Hookworm and S. stercoralis infection were determined from collected fecal samples of all subjects using either Baermann test, Koga Agar Plate (KAP), or Harada- Mori culture method. The prevalence of hookworm and S. stercoralis infection was 8.82%, and 0,42%. Correlation between nutritional status and hookworm infection were analyzed by Mann-Whitney test with p value = 0.54 (p > 0.05). Prevalence of hookworm and S. stercoralis among children under five years in Kokar were 8.82% and 0.42%. There was no significant correlation between nutritional status with hookworm infection prevalence.
{"title":"Correlation of Nutritional Status with Hookworm and Strongyloides stercoralis Infection in Children Under Five Years in Kokar Public Health Center, Alor Regency, East Nusa Tenggara","authors":"Benaya Yamin Onesiforus, Indra Elisabet Lalangpuling, M. A. Wijayanti, E. H. Murhandarwati","doi":"10.20473/ijtid.v8i3.12570","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.12570","url":null,"abstract":"Malnutrition can reduce immune response particularly in cytokine (IL-4, IL-5, IL-10) production and immune effector (eosinophil, IgE, and mast cell), thus increasing the probability of intestinal nematode infection. Through this study, intestinal nematode infections occurred among children under five years, at different nutrition status, in Kokar Public Health center, Alor Regency, East Nusa Tenggara was captured. Hookworm and Strongyloides stercoralis were studied as both of them have devastating impacts compare to other helminthes compare to other helminths. This study is a cross- sectional study with a quote sampling technique. As many as 238 children, aged 12-59 months living in Kokar’s Public Health Center area, Alor regency were recruited in this study i.e. 7.7% severely underweight, 19.2% underweight, 70.5% normal and 2.6% overweight. Data were collected in August - October 2016. Hookworm and S. stercoralis infection were determined from collected fecal samples of all subjects using either Baermann test, Koga Agar Plate (KAP), or Harada- Mori culture method. The prevalence of hookworm and S. stercoralis infection was 8.82%, and 0,42%. Correlation between nutritional status and hookworm infection were analyzed by Mann-Whitney test with p value = 0.54 (p > 0.05). Prevalence of hookworm and S. stercoralis among children under five years in Kokar were 8.82% and 0.42%. There was no significant correlation between nutritional status with hookworm infection prevalence.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43743600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.17553
Agusta Reny Soekoyo, S. Sulistiawati, Wahyu Setyorini, K. Kuntaman
Livestock would be a risk factor of resistant bacteria that impact on human health. Rural area with farms as major economic source has become a risk of the spread of the ESBL producing Enterobacteriaceae The aim of the study was to explore the distribution and risk factor of ESBL (extended-spectrum β-lactamase) producing Enterobacteriaceae in the gut bacterial flora of dairy cows and people surrounding farming area. Total of 204 fecal swab samples were collected, 102 from dairy cows and 102 from farmers. Samples were sub-cultured by streaking on MacConkey agar supplemented with 2 mg/L cefotaxime. The growing colonies were confirmed of the ESBL producer by Modified Double Disk Test (M-DDST) and identification of Enterobacteriaceae by biochemical test. ESBL genes were identified by PCR. ESBL producing bacteria were found 13.7% in dairy cows and 34.3% in farmers. ESBL producing Enterobacteriaceae in dairy cows were 6.9% and in farmers of 33.3%. Statistical analysis showed: Distribution of ESBL producing Enterobacteriaceae strain were insignificant among dairy cows and farmers while blaTEM distribution was significantly different (p= 0,035) and use of antibiotic was identified as a risk factor of colonization of ESBL producing Enterobacteriaceae in farmers (p= 0,007). Farmers had suspected as the source of ESBL producing Enterobacteriaceae based on higher prevalence. Further education of appropriate use of antibiotic need to enhance to control risk factor and prevent the colonization of ESBL producing Enterobacteriaceae
{"title":"The Epidemiological Pattern and Risk Factor of ESBL (Extended Spectrum Β-Lactamase) Producing Enterobacteriaceae in Gut Bacterial Flora of Dairy Cows and People Surrounding in Rural Area, Indonesia","authors":"Agusta Reny Soekoyo, S. Sulistiawati, Wahyu Setyorini, K. Kuntaman","doi":"10.20473/ijtid.v8i3.17553","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.17553","url":null,"abstract":"Livestock would be a risk factor of resistant bacteria that impact on human health. Rural area with farms as major economic source has become a risk of the spread of the ESBL producing Enterobacteriaceae The aim of the study was to explore the distribution and risk factor of ESBL (extended-spectrum β-lactamase) producing Enterobacteriaceae in the gut bacterial flora of dairy cows and people surrounding farming area. Total of 204 fecal swab samples were collected, 102 from dairy cows and 102 from farmers. Samples were sub-cultured by streaking on MacConkey agar supplemented with 2 mg/L cefotaxime. The growing colonies were confirmed of the ESBL producer by Modified Double Disk Test (M-DDST) and identification of Enterobacteriaceae by biochemical test. ESBL genes were identified by PCR. ESBL producing bacteria were found 13.7% in dairy cows and 34.3% in farmers. ESBL producing Enterobacteriaceae in dairy cows were 6.9% and in farmers of 33.3%. Statistical analysis showed: Distribution of ESBL producing Enterobacteriaceae strain were insignificant among dairy cows and farmers while blaTEM distribution was significantly different (p= 0,035) and use of antibiotic was identified as a risk factor of colonization of ESBL producing Enterobacteriaceae in farmers (p= 0,007). Farmers had suspected as the source of ESBL producing Enterobacteriaceae based on higher prevalence. Further education of appropriate use of antibiotic need to enhance to control risk factor and prevent the colonization of ESBL producing Enterobacteriaceae","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41365415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.16796
Eko Oktiawan Wicaksono, Artaria Tjempakasari, W. Widodo
Chronic kidney disease (CKD) is now a global epidemic, and the prevalence is increasing worldwide. Hemodialysis is one of the ways to treat by kidney function replacement. Infection is the number two cause of death in patients with hemodialysis (HD). Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of bacteriemia in patients with dialysis. The epidemiological data of MRSA carriers in CKD in Indonesia are still scarce. This study was to determine the prevalence of MRSA carriers in patients at The Kidney and Hypertension Outpatient-clinic and Hemodialysis Installation at Dr. Soetomo Academic General Hospital, Surabaya Indonesia. The study design was descriptive-analytic with a cross- sectional study design. Sampling was collected consecutively. Data on the general characteristics of the research subjects will be analyzed using a Chi-Squared test. There were 150 CKD stage five patients included in this study, the number of patients has MRSA carrier were 6 (4%), among them, subjects underwent HD MRSA carrier were 2 subjects(2.7%), while for non-HD patients with MRSA were 4 subjects (5.3 %). There were no significant differences in MRSA carriers between HD and non HD groups (p=0.404). Comorbid factors that accompany MRSA carriers are diabetes mellitus, hypertension, kidney stones, gout, and systemic lupus erythematosus (SLE). This study found, there were no significant differences in the incidence of MRSA carriers in stage five CKD non HD or HD groups. MRSA colonization exists in stage five CKD sufferers, so awareness of MRSA colonization
{"title":"Prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) Carrier in Hemodialysis Patients at Dr. Soetomo Academic General Hospital","authors":"Eko Oktiawan Wicaksono, Artaria Tjempakasari, W. Widodo","doi":"10.20473/ijtid.v8i3.16796","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.16796","url":null,"abstract":"Chronic kidney disease (CKD) is now a global epidemic, and the prevalence is increasing worldwide. Hemodialysis is one of the ways to treat by kidney function replacement. Infection is the number two cause of death in patients with hemodialysis (HD). Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of bacteriemia in patients with dialysis. The epidemiological data of MRSA carriers in CKD in Indonesia are still scarce. This study was to determine the prevalence of MRSA carriers in patients at The Kidney and Hypertension Outpatient-clinic and Hemodialysis Installation at Dr. Soetomo Academic General Hospital, Surabaya Indonesia. The study design was descriptive-analytic with a cross- sectional study design. Sampling was collected consecutively. Data on the general characteristics of the research subjects will be analyzed using a Chi-Squared test. There were 150 CKD stage five patients included in this study, the number of patients has MRSA carrier were 6 (4%), among them, subjects underwent HD MRSA carrier were 2 subjects(2.7%), while for non-HD patients with MRSA were 4 subjects (5.3 %). There were no significant differences in MRSA carriers between HD and non HD groups (p=0.404). Comorbid factors that accompany MRSA carriers are diabetes mellitus, hypertension, kidney stones, gout, and systemic lupus erythematosus (SLE). This study found, there were no significant differences in the incidence of MRSA carriers in stage five CKD non HD or HD groups. MRSA colonization exists in stage five CKD sufferers, so awareness of MRSA colonization","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41551027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.16084
Febriana Aquaresta, A. P. Kawilarang, P. Endraswari
Invasive candidiasis is an important health-care-associated fungal infection. Candida is often described as an opportunistic pathogen. It is commensal flora in the gastrointestinal tract. Invasive candidiasis can happen usually because of a consequence of increased or abnormal colonization together with a local or generalized defect in host defenses. Candidiasis can occur in patients with HIV, therapy with a broad-spectrum antibiotic, transplant organ, and immunocompromised. Most cases of gastric perforation occur as complications of Peptic Ulcer Disease (PUD), Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) and gastric neoplasms, but candidiasis as a cause of gastric perforation is very rare. This study aims to reveal the correlation between gastric perforation with candidiasis and NSAIDs. It was reported that a 57-year-old East Java Indonesian female presented with severe epigastric pain, generalized peritonitis, fever, nausea also vomiting and had a history of NSAIDs used for five years. The patient was taken to the general surgery of Dr. Sutomo Surabaya Hospital and performed exploratory laparotomy. A gastric perforation was discovered in the antrum. Microbiology culture examination from biopsy gastric tissue revealed an intense fungal growth from sabouraudagar medium and there is no other microorganism that grew in aerobic culture. Candida albicans was identified by VITEK® 2 COMPACT. Histopathological examination from biopsy gastric tissue was performed by Olympus CX-21 microscope, showed invasive Candida albicans consisting of numerous fungal yeasts and pseudohyphae invading and destroying the gastric wall. The patient was subsequently treated with fluconazole anti-fungal and discharge home after nine days postoperative period in good condition. From this result, we suggest using an antifungal treatment for patients who use NSAIDs for long periods to prevent candidiasis.
{"title":"Gastric Perforation Associated with Candidiasis and NSAIDS","authors":"Febriana Aquaresta, A. P. Kawilarang, P. Endraswari","doi":"10.20473/ijtid.v8i3.16084","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.16084","url":null,"abstract":"Invasive candidiasis is an important health-care-associated fungal infection. Candida is often described as an opportunistic pathogen. It is commensal flora in the gastrointestinal tract. Invasive candidiasis can happen usually because of a consequence of increased or abnormal colonization together with a local or generalized defect in host defenses. Candidiasis can occur in patients with HIV, therapy with a broad-spectrum antibiotic, transplant organ, and immunocompromised. Most cases of gastric perforation occur as complications of Peptic Ulcer Disease (PUD), Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) and gastric neoplasms, but candidiasis as a cause of gastric perforation is very rare. This study aims to reveal the correlation between gastric perforation with candidiasis and NSAIDs. It was reported that a 57-year-old East Java Indonesian female presented with severe epigastric pain, generalized peritonitis, fever, nausea also vomiting and had a history of NSAIDs used for five years. The patient was taken to the general surgery of Dr. Sutomo Surabaya Hospital and performed exploratory laparotomy. A gastric perforation was discovered in the antrum. Microbiology culture examination from biopsy gastric tissue revealed an intense fungal growth from sabouraudagar medium and there is no other microorganism that grew in aerobic culture. Candida albicans was identified by VITEK® 2 COMPACT. Histopathological examination from biopsy gastric tissue was performed by Olympus CX-21 microscope, showed invasive Candida albicans consisting of numerous fungal yeasts and pseudohyphae invading and destroying the gastric wall. The patient was subsequently treated with fluconazole anti-fungal and discharge home after nine days postoperative period in good condition. From this result, we suggest using an antifungal treatment for patients who use NSAIDs for long periods to prevent candidiasis.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43091253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.11776
T. H. Sucipto, A. Wibrianto, F. Martak, Siti Churrotin, I. H. Amarullah, H. Setyawati, Puspa Wardhani, A. Aryati, S. Soegijanto
Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many efforts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 μg/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections
{"title":"Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell","authors":"T. H. Sucipto, A. Wibrianto, F. Martak, Siti Churrotin, I. H. Amarullah, H. Setyawati, Puspa Wardhani, A. Aryati, S. Soegijanto","doi":"10.20473/ijtid.v8i3.11776","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.11776","url":null,"abstract":"Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many efforts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 μg/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47316638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.20473/ijtid.v8i3.17961
Sp.P Laksmi Wulandari, Putri Mega Juwita
Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis (Mtb) of which attacking various organs particularly the lungs. Tuberculosis can occur together with malignancy or manifest as malignancy. Lung tuberculosis may appear in a variety of clinical and radiological manifestations caused by other diseases including tumors. These tumors are called pseudo-tumors. TB pseudo-tumor is a rare manifestation that can occur in immunocompetent patients in both primary and post-primary TB. The clinical presentation of TB pseudo-tumor is nonspecifi c and the clinical suspicion must be increased to diagnose related diseases. Radiological features can also be challenging to be distinguished from actual tumors. The classic manifestations of pulmonary TB are generally easy to diagnose due to the distinctive clinical and radiological characteristics nonetheless some pulmonary TB symptoms are also often found in patients with lung cancer. Infection patients resemble malignancies most were asymptomatic (> 27%) and the remaining 27% showed symptoms that varied with the average symptoms experienced about 1 month earlier. Clinical presentations that require a lot of misdiagnosis result in delayed treatment and unnecessary procedures. Establishing a diagnosis in cases of tuberculosis that causes malignancy is very important since the management and outcomes of the infection and malignancy process are quite diff erent. Consequently we report a 24-year-old man with tuberculosis possible lung cancer with multiple bone metastase. Extrapulmonary tuberculosis which attacks bones and joints constitutes 10% to 20% of all TB cases. The location of bone and joint TB generally develops in the lumbar or thoracic vertebrae.
{"title":"Disseminated Tuberculosis Mimicking Lung Cancer with Multiple Bone Metastasis: A Case Report","authors":"Sp.P Laksmi Wulandari, Putri Mega Juwita","doi":"10.20473/ijtid.v8i3.17961","DOIUrl":"https://doi.org/10.20473/ijtid.v8i3.17961","url":null,"abstract":"Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis (Mtb) of which attacking various organs particularly the lungs. Tuberculosis can occur together with malignancy or manifest as malignancy. Lung tuberculosis may appear in a variety of clinical and radiological manifestations caused by other diseases including tumors. These tumors are called pseudo-tumors. TB pseudo-tumor is a rare manifestation that can occur in immunocompetent patients in both primary and post-primary TB. The clinical presentation of TB pseudo-tumor is nonspecifi c and the clinical suspicion must be increased to diagnose related diseases. Radiological features can also be challenging to be distinguished from actual tumors. The classic manifestations of pulmonary TB are generally easy to diagnose due to the distinctive clinical and radiological characteristics nonetheless some pulmonary TB symptoms are also often found in patients with lung cancer. Infection patients resemble malignancies most were asymptomatic (> 27%) and the remaining 27% showed symptoms that varied with the average symptoms experienced about 1 month earlier. Clinical presentations that require a lot of misdiagnosis result in delayed treatment and unnecessary procedures. Establishing a diagnosis in cases of tuberculosis that causes malignancy is very important since the management and outcomes of the infection and malignancy process are quite diff erent. Consequently we report a 24-year-old man with tuberculosis possible lung cancer with multiple bone metastase. Extrapulmonary tuberculosis which attacks bones and joints constitutes 10% to 20% of all TB cases. The location of bone and joint TB generally develops in the lumbar or thoracic vertebrae.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47529359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-31DOI: 10.20473/ijtid.v8i2.16027
Erika Marfiani, Jusri Ichwani, Novira Widajanti, Daniel Maranatha, M. Amin
Elderly are especially prone to the adverse health effects of chronic obstructive pulmonary disease (COPD). COPD is a typical aging disease which is found to be about 12% in the age group more than 64 years old. Frailty is an important geriatics syndrome, while adiponectin is an important adipokine that regulate homeostasis of energy. Adiponectin is affected by age, especially in males. Adiponectin levels increases as subcutaneous fat cells in older men decreases. Increased adiponectin can lead to muscle wasting which will further reduce body weight and body mass index (BMI), which indirectly also increases the degree of frailty. Until now the relationship between adiponectin with frailty degree in advanced COPD is still unknown.The aims of this study was to investigate the relationship between plasma adiponectin level and frailty in COPD elders. This was an observational analytic cross-sectional study. All anthropometric parameters, including weight, height, and body mass index (BMI), were measured. Adiponectin was measured by ELISA methods obtained from venous blood samples. Aged more than or equal to 60 years old, the patients underwent spirometry to determine the degree of airflow limitation and the degree of frailty defined by the Fried criteria. Statistic analysis used Rank Spearman. Thirty-eight male COPD patients became the subject of the study. The average age was 70-74 years, with a total of 13 robust, 12 prefrails and 13 frail patients. Level of adiponectin (mean and SD) in robust, prefrail, and frail were 6.84+ 2.66 , 6.58 + 4.27, and 11.62 + 4.90 respectively, p=0.015. Further analysis showed that level of adiponectin rose progresively with an increasing number of components of frailty. The degree of obstruction mostly with mild (42.1%), and no subjects with very severe. There were an increase in serum adiponectin levels in all subjects. In conclusion, level of adiponectin serum correlates positively with the degree of frailty.
{"title":"RELATIONSHIP BETWEEN LEVEL OF SERUM ADIPONECTIN AND FRAILTY IN ELDERLY PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE","authors":"Erika Marfiani, Jusri Ichwani, Novira Widajanti, Daniel Maranatha, M. Amin","doi":"10.20473/ijtid.v8i2.16027","DOIUrl":"https://doi.org/10.20473/ijtid.v8i2.16027","url":null,"abstract":"Elderly are especially prone to the adverse health effects of chronic obstructive pulmonary disease (COPD). COPD is a typical aging disease which is found to be about 12% in the age group more than 64 years old. Frailty is an important geriatics syndrome, while adiponectin is an important adipokine that regulate homeostasis of energy. Adiponectin is affected by age, especially in males. Adiponectin levels increases as subcutaneous fat cells in older men decreases. Increased adiponectin can lead to muscle wasting which will further reduce body weight and body mass index (BMI), which indirectly also increases the degree of frailty. Until now the relationship between adiponectin with frailty degree in advanced COPD is still unknown.The aims of this study was to investigate the relationship between plasma adiponectin level and frailty in COPD elders. This was an observational analytic cross-sectional study. All anthropometric parameters, including weight, height, and body mass index (BMI), were measured. Adiponectin was measured by ELISA methods obtained from venous blood samples. Aged more than or equal to 60 years old, the patients underwent spirometry to determine the degree of airflow limitation and the degree of frailty defined by the Fried criteria. Statistic analysis used Rank Spearman. Thirty-eight male COPD patients became the subject of the study. The average age was 70-74 years, with a total of 13 robust, 12 prefrails and 13 frail patients. Level of adiponectin (mean and SD) in robust, prefrail, and frail were 6.84+ 2.66 , 6.58 + 4.27, and 11.62 + 4.90 respectively, p=0.015. Further analysis showed that level of adiponectin rose progresively with an increasing number of components of frailty. The degree of obstruction mostly with mild (42.1%), and no subjects with very severe. There were an increase in serum adiponectin levels in all subjects. In conclusion, level of adiponectin serum correlates positively with the degree of frailty.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48870699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-31DOI: 10.20473/ijtid.v8i2.12657
A. Widyawaruyanti, A. A. Permanasari, Laila Nur Hidayatus, L. Tumewu, T. Wahyuni, A. Hafid
Hepatitis C Virus (HCV) infection is a serious public health problem since HCV is the ribonucleic acid (RNA) virus that easy to mutate. The HCV standard treatment has rapidly developed but the possibility of resistance and effectiveness of treatment needs to be considered. The medicinal plants are a source of various compounds that may potentially cure diseases including infectious diseases. Since a long years ago, medicinal plants were famous as an inherited treatment that believed to cure the disease. One of the medicinal plants is Scoparia dulcis (S. dulcis) that belongs to Scrophulariaceae family and traditionally used as remedies for digestive problems, hypertension, diabetes mellitus, bronchitis, and as an analgesic & antipyretic agent. The previous report showed that S. dulcis was known active as an antiviral against Herpes Simplex Virus (HSV) type 1 in vitro and in vivo. The aim of the study is to determine the biactivity potential of S. dulcis against HCV. Scoparia dulcis was extracted using 80% ethanol (EE) then further separated by liquid-liquid fractionation using dichloromethane (DCMF), ethyl acetate (EAF), butanol solvent (BF) and water (WF). The in vitro anti-HCV analysis was performed with Huh7it cells and HCV JFH1 (genotype 2a) by determining i nhibition concentration 50 (IC 50 ). The toxicity (Cytotoxicity Con c entration 50, CC 50 ) test wa s performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and mechanism of action were analyzed using time addition experiment. Phytochemical groups as the suspected active compounds of S. dulcis were identified by Thin Layer Chromatography (TLC) and observed under UV 254 nm, UV 365 nm, before and after sprayed using H 2 SO 4 10% and heated at 105 o C for 5 min utes. The IC 50 test result of 80% EE and DCMF showed anti-HCV activity with a value of 12.7±4.8 µg/ml and 5.8±0.69 µg/ml , while EAF, BF, and AF respectively resulted in IC 50 value of >100 µg/ml that suggested there was no inhibition effect on HCV JFH1 . The DCMF was the most active fraction but toxic to the cell with CC 50 value >23 µg/ml and selectivity index (SI) >3.9. According to the time addition experiment data, DCMF of S. dulcis inhibited post entry step HCV JFH1 infection that it means the possibility was to inhibit virus replication and or virion release. Scoparia dulcis contain chlorophyll, flavonoids and terpenoids as the suspected active compounds for inhibition of HCV JFH1 infecton . Futher study of post-entry inhibitions of HCV infection was needed.
{"title":"ANTI HEPATITIS C ACTIVITY AND TOXICITY OF Scoparia dulcis LINN. HERB","authors":"A. Widyawaruyanti, A. A. Permanasari, Laila Nur Hidayatus, L. Tumewu, T. Wahyuni, A. Hafid","doi":"10.20473/ijtid.v8i2.12657","DOIUrl":"https://doi.org/10.20473/ijtid.v8i2.12657","url":null,"abstract":"Hepatitis C Virus (HCV) infection is a serious public health problem since HCV is the ribonucleic acid (RNA) virus that easy to mutate. The HCV standard treatment has rapidly developed but the possibility of resistance and effectiveness of treatment needs to be considered. The medicinal plants are a source of various compounds that may potentially cure diseases including infectious diseases. Since a long years ago, medicinal plants were famous as an inherited treatment that believed to cure the disease. One of the medicinal plants is Scoparia dulcis (S. dulcis) that belongs to Scrophulariaceae family and traditionally used as remedies for digestive problems, hypertension, diabetes mellitus, bronchitis, and as an analgesic & antipyretic agent. The previous report showed that S. dulcis was known active as an antiviral against Herpes Simplex Virus (HSV) type 1 in vitro and in vivo. The aim of the study is to determine the biactivity potential of S. dulcis against HCV. Scoparia dulcis was extracted using 80% ethanol (EE) then further separated by liquid-liquid fractionation using dichloromethane (DCMF), ethyl acetate (EAF), butanol solvent (BF) and water (WF). The in vitro anti-HCV analysis was performed with Huh7it cells and HCV JFH1 (genotype 2a) by determining i nhibition concentration 50 (IC 50 ). The toxicity (Cytotoxicity Con c entration 50, CC 50 ) test wa s performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and mechanism of action were analyzed using time addition experiment. Phytochemical groups as the suspected active compounds of S. dulcis were identified by Thin Layer Chromatography (TLC) and observed under UV 254 nm, UV 365 nm, before and after sprayed using H 2 SO 4 10% and heated at 105 o C for 5 min utes. The IC 50 test result of 80% EE and DCMF showed anti-HCV activity with a value of 12.7±4.8 µg/ml and 5.8±0.69 µg/ml , while EAF, BF, and AF respectively resulted in IC 50 value of >100 µg/ml that suggested there was no inhibition effect on HCV JFH1 . The DCMF was the most active fraction but toxic to the cell with CC 50 value >23 µg/ml and selectivity index (SI) >3.9. According to the time addition experiment data, DCMF of S. dulcis inhibited post entry step HCV JFH1 infection that it means the possibility was to inhibit virus replication and or virion release. Scoparia dulcis contain chlorophyll, flavonoids and terpenoids as the suspected active compounds for inhibition of HCV JFH1 infecton . Futher study of post-entry inhibitions of HCV infection was needed.","PeriodicalId":13538,"journal":{"name":"Indonesian Journal of Tropical and Infectious Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45828808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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