Pub Date : 2025-08-11DOI: 10.1016/j.ibiod.2025.106188
Inês Silva , Cátia Salvador , Ana Z. Miller , António Candeias , Ana Teresa Caldeira
Biodeterioration poses a major challenge to the conservation of stone monuments, particularly in UNESCO World Heritage sites like the Convent of Christ and the Batalha Monastery in Portugal. This study introduces novel sampling locations in both monuments, allowing a more targeted characterization of the eukaryotic microbial communities colonizing the Convent of Christ's stone surfaces. Microbiota distribution across distinct zones revealed that key lichenized fungi with biodeteriogenic potential—Dirina, Xanthoria, Purpureocillium, Verrucaria, and Cystocoleus—were predominant, as identified by Next Generation Sequencing (NGS). Additionally, an orange-pink bacterial biofilm from the Batalha Monastery was analyzed through colorimetric techniques and antimicrobial assays, demonstrating that Biotin-T was the most effective treatment, inhibiting bacterial growth while preserving the stone's original appearance and progressively reducing microbial viability over time. These findings provide insights into biodeterioration processes and offer potential solutions for the conservation of historic stone heritage.
{"title":"Addressing biodeterioration at UNESCO stone monuments: Tomar convent and Batalha Monastery","authors":"Inês Silva , Cátia Salvador , Ana Z. Miller , António Candeias , Ana Teresa Caldeira","doi":"10.1016/j.ibiod.2025.106188","DOIUrl":"10.1016/j.ibiod.2025.106188","url":null,"abstract":"<div><div>Biodeterioration poses a major challenge to the conservation of stone monuments, particularly in UNESCO World Heritage sites like the Convent of Christ and the Batalha Monastery in Portugal. This study introduces novel sampling locations in both monuments, allowing a more targeted characterization of the eukaryotic microbial communities colonizing the Convent of Christ's stone surfaces. Microbiota distribution across distinct zones revealed that key lichenized fungi with biodeteriogenic potential—<em>Dirina</em>, <em>Xanthoria</em>, <em>Purpureocillium</em>, <em>Verrucaria</em>, and <em>Cystocoleus</em>—were predominant, as identified by Next Generation Sequencing (NGS). Additionally, an orange-pink bacterial biofilm from the Batalha Monastery was analyzed through colorimetric techniques and antimicrobial assays, demonstrating that Biotin-T was the most effective treatment, inhibiting bacterial growth while preserving the stone's original appearance and progressively reducing microbial viability over time. These findings provide insights into biodeterioration processes and offer potential solutions for the conservation of historic stone heritage.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106188"},"PeriodicalIF":4.1,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-06DOI: 10.1016/j.ibiod.2025.106185
Jiacheng Zou, Jingru Li, Caihong Yu, Banghua He, Halimier Duman, Zhanbin Huang, Yan Ma, Shuaixian Mao, Jinshuai Shi
Public health and ecological security are seriously threatened by hexavalent chromium [Cr(Ⅵ)], whereas bioreduction of Cr(Ⅵ) to trivalent chromium [Cr(Ⅲ)] presents a far lesser risk to the environment. The study examined the performance and removal mechanisms of Cr(VI) by Alcaligenes faecalis S1, known for its strong reducing capacity, and Lysinibacillus macrolides S2, which shows tolerance to Cr(VI). The results indicated that the extracellular secretions of strains S1 and S2 were the main sites of Cr(VI) reduction, primarily in the form of extracellular reduction of hexavalent chromium to trivalent chromium. The genomic findings of bacteria substantiated this idea, as both strains S1 and S2 possessed chromate reduction genes, concurrently demonstrating chromate transporter proteins and DNA repair proteases, among others, that function in reducing Cr(VI) toxicity to themselves. The construction of bacterial consortium S3 was further pursued in order to investigate the synergistic resistance mechanism of strains S1 and S2 against Cr(VI). It was found that S1 and S2 synergistically enhance the Cr(VI) resistance of the bacterial consortium S3 through the expression of reduction and resistance genes, endowing it with broader pH adaptability and excellent Cr(VI) reduction capabilities. This complementary effect allowed S3 to have multiple biological reduction mechanisms: enzyme-mediated reduction of Cr(VI), DNA-repaired enzymes, detoxification enzymes, and Cr(VI) efflux, significantly reducing Cr(VI) toxicity damage. In conclusion, this study has deepened the understanding of the fundamental characteristics of strains S1 and S2, as well as the remediation synergistic mechanisms of Cr(VI), providing a molecular basis and scientific guidance for future bioremediation.
{"title":"Exploring synergistic interactions in Cr(VI) removal by highly Cr(VI)-reductive strain Alcaligenes faecalis S1 and Cr(VI)-tolerant strain Lysinibacillus macrolides S2: genomic and mechanistic insights","authors":"Jiacheng Zou, Jingru Li, Caihong Yu, Banghua He, Halimier Duman, Zhanbin Huang, Yan Ma, Shuaixian Mao, Jinshuai Shi","doi":"10.1016/j.ibiod.2025.106185","DOIUrl":"10.1016/j.ibiod.2025.106185","url":null,"abstract":"<div><div>Public health and ecological security are seriously threatened by hexavalent chromium [Cr(Ⅵ)], whereas bioreduction of Cr(Ⅵ) to trivalent chromium [Cr(Ⅲ)] presents a far lesser risk to the environment. The study examined the performance and removal mechanisms of Cr(VI) by <em>Alcaligenes faecalis</em> S1, known for its strong reducing capacity, and <em>Lysinibacillus macrolides</em> S2, which shows tolerance to Cr(VI). The results indicated that the extracellular secretions of strains S1 and S2 were the main sites of Cr(VI) reduction, primarily in the form of extracellular reduction of hexavalent chromium to trivalent chromium. The genomic findings of bacteria substantiated this idea, as both strains S1 and S2 possessed chromate reduction genes, concurrently demonstrating chromate transporter proteins and DNA repair proteases, among others, that function in reducing Cr(VI) toxicity to themselves. The construction of bacterial consortium S3 was further pursued in order to investigate the synergistic resistance mechanism of strains S1 and S2 against Cr(VI). It was found that S1 and S2 synergistically enhance the Cr(VI) resistance of the bacterial consortium S3 through the expression of reduction and resistance genes, endowing it with broader pH adaptability and excellent Cr(VI) reduction capabilities. This complementary effect allowed S3 to have multiple biological reduction mechanisms: enzyme-mediated reduction of Cr(VI), DNA-repaired enzymes, detoxification enzymes, and Cr(VI) efflux, significantly reducing Cr(VI) toxicity damage. In conclusion, this study has deepened the understanding of the fundamental characteristics of strains S1 and S2, as well as the remediation synergistic mechanisms of Cr(VI), providing a molecular basis and scientific guidance for future bioremediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106185"},"PeriodicalIF":4.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-05DOI: 10.1016/j.ibiod.2025.106182
Jing Hu , Bowen Peng , Zao Liu , Tong Long , Wa Gao , Yongze Wang , Xiaonan Liu , Jinfang Zhao
The biodegradation of polyethylene (PE) presents a promising approach to resolve plastic waste pollution, yet research achievements in this field are relatively scarce. In this study, two PE-degrading strains, Klebsiella variicola ZB-1 and Pseudomonas fulva ZH-2, were isolated from the gut microbiota of Spodoptera frugiperda larvae, and ZB-1 achieved a remarkable 9.79 ± 1.41 % weight loss of low-density polyethylene (LDPE) within 35 days when fed with PE powder. Scanning electron microscopy and water contact angle measurements revealed that these strains could adhere to PE films and render them hydrophilic. Fourier-transform infrared spectroscopy analysis showed an increase in the oxygen-carbon ratio of the PE films, facilitating degradation. In addition, genome sequencing was utilized to explore the underlying PE degradation mechanisms, and a laccase-like multicopper oxidase (CueO) was demonstrated to have obvious PE degradation capability for the first time. This enzyme exhibited outstanding pH stability (optimal pH 2.5) and temperature tolerance (55 °C), with activity further enhanced by Cu2+/Mn2+ ions. Comprehensive characterization using SEM, FTIR, and WCA systematically revealed biofilm-mediated surface erosion and polymer chain destabilization mechanisms. Overall, this study established a novel biological resource library for PE degradation by exploring the genetic information of PE-degrading microorganisms and their key enzyme systems, laying a theoretical foundation for elucidating the molecular mechanisms of polyolefin biodepolymerization.
{"title":"Polyethylene degradation mediated by Klebsiella variicola isolated from the gut of insect larvae","authors":"Jing Hu , Bowen Peng , Zao Liu , Tong Long , Wa Gao , Yongze Wang , Xiaonan Liu , Jinfang Zhao","doi":"10.1016/j.ibiod.2025.106182","DOIUrl":"10.1016/j.ibiod.2025.106182","url":null,"abstract":"<div><div>The biodegradation of polyethylene (PE) presents a promising approach to resolve plastic waste pollution, yet research achievements in this field are relatively scarce. In this study, two PE-degrading strains, <em>Klebsiella variicola</em> ZB-1 and <em>Pseudomonas fulva</em> ZH-2, were isolated from the gut microbiota of <em>Spodoptera frugiperda</em> larvae, and ZB-1 achieved a remarkable 9.79 ± 1.41 % weight loss of low-density polyethylene (LDPE) within 35 days when fed with PE powder. Scanning electron microscopy and water contact angle measurements revealed that these strains could adhere to PE films and render them hydrophilic. Fourier-transform infrared spectroscopy analysis showed an increase in the oxygen-carbon ratio of the PE films, facilitating degradation. In addition, genome sequencing was utilized to explore the underlying PE degradation mechanisms, and a laccase-like multicopper oxidase (CueO) was demonstrated to have obvious PE degradation capability for the first time. This enzyme exhibited outstanding pH stability (optimal pH 2.5) and temperature tolerance (55 °C), with activity further enhanced by Cu<sup>2+</sup>/Mn<sup>2+</sup> ions. Comprehensive characterization using SEM, FTIR, and WCA systematically revealed biofilm-mediated surface erosion and polymer chain destabilization mechanisms. Overall, this study established a novel biological resource library for PE degradation by exploring the genetic information of PE-degrading microorganisms and their key enzyme systems, laying a theoretical foundation for elucidating the molecular mechanisms of polyolefin biodepolymerization.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106182"},"PeriodicalIF":4.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144773226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-28DOI: 10.1016/j.ibiod.2025.106172
Xule Pan , Hao Huang , Yian Wang , Xinping Wang , Xiuwen Ren , Renren Wu , Mutai Bao , Jinpeng Wang , Haoshuai Li
The Yellow River Delta's coastal wetlands represent a vital blue carbon ecosystem, which approximately buries 2087 ± 251 g C/(m2∙yr) and the carbon stock 16.7–21.0 Mg C/ha), significantly contributing to both global carbon cycles and China's regional carbon neutrality goals. However, the TOC decreased in past few years (1999–2023). This review systematically evaluates the key drivers of carbon sequestration, burial mechanisms, and prevalent assessment techniques applied in the region. Special attention is given to the interactions among sediment dynamics, vegetation types, and anthropogenic influences. The synergistic effect of exogenous carbon inputs from the Yellow River and the high productivity of native salt marsh vegetation creates a unique pattern of organic carbon burial. Advanced monitoring approaches, such as the Surface Elevation Table–Marker Horizon (SET-MH) system and the stock difference method, are recommended to quantify fine-scale depositional processes and their contribution to sedimentary carbon stocks. Moreover, the integration of biomarker analysis and eddy covariance flux measurements improves carbon budgeting by enhancing source identification and spatiotemporal flux monitoring. Among environmental factors, freshwater inflow is critical for increasing carbon sequestration efficiency by mitigating soil salinity, whereas frequent redox cycles triggered by tidal flat resuspension hinder organic carbon preservation. Although Spartina alterniflora enhances localized soil organic carbon (SOC) content, it does not significantly increase total SOC stocks and instead undermines ecosystem stability by displacing native species. The review also outlines degradation threats and recommends blue carbon management strategies, including legal restrictions on reclamation and trial implementation of carbon trading mechanisms. Overall, this work reinforces the strategic value of the Yellow River Delta in advancing sustainable coastal wetland management and global carbon neutrality targets.
黄河三角洲滨海湿地是一个重要的蓝碳生态系统，其碳掩埋量约为2087±251 g C/(m2∙yr)，碳储量为16.7 ~ 21.0 Mg C/ha，对全球碳循环和中国区域碳中和目标都有重要贡献。然而，TOC在过去几年（1999-2023）有所下降。本文系统地评价了该地区碳固存的主要驱动因素、埋藏机制和常用的评估技术。特别注意泥沙动力学、植被类型和人为影响之间的相互作用。黄河外源碳输入与盐沼原生植被高产能的协同效应形成了独特的有机碳埋藏模式。建议采用先进的监测方法，如地面高程表-标记-水平（SET-MH）系统和储量差法，量化精细尺度沉积过程及其对沉积碳储量的贡献。此外，生物标志物分析和涡旋相关通量测量的整合通过加强源识别和时空通量监测来改善碳预算。在环境因素中，淡水流入是通过降低土壤盐分来提高固碳效率的关键，而潮滩再悬浮引发的频繁氧化还原循环则阻碍了有机碳的保存。互花米草虽然提高了局部土壤有机碳含量，但并没有显著增加总有机碳储量，反而通过取代本地物种破坏了生态系统的稳定性。该报告还概述了退化威胁，并建议了蓝碳管理战略，包括对开垦的法律限制和试行碳交易机制。总的来说，这项工作加强了黄河三角洲在推进可持续沿海湿地管理和全球碳中和目标方面的战略价值。
{"title":"Carbon stocks in coastal wetlands of the Yellow River Delta and their environmental influencing factors","authors":"Xule Pan , Hao Huang , Yian Wang , Xinping Wang , Xiuwen Ren , Renren Wu , Mutai Bao , Jinpeng Wang , Haoshuai Li","doi":"10.1016/j.ibiod.2025.106172","DOIUrl":"10.1016/j.ibiod.2025.106172","url":null,"abstract":"<div><div>The Yellow River Delta's coastal wetlands represent a vital blue carbon ecosystem, which approximately buries 2087 ± 251 g C/(m<sup>2</sup>∙yr) and the carbon stock 16.7–21.0 Mg C/ha), significantly contributing to both global carbon cycles and China's regional carbon neutrality goals. However, the TOC decreased in past few years (1999–2023). This review systematically evaluates the key drivers of carbon sequestration, burial mechanisms, and prevalent assessment techniques applied in the region. Special attention is given to the interactions among sediment dynamics, vegetation types, and anthropogenic influences. The synergistic effect of exogenous carbon inputs from the Yellow River and the high productivity of native salt marsh vegetation creates a unique pattern of organic carbon burial. Advanced monitoring approaches, such as the Surface Elevation Table–Marker Horizon (SET-MH) system and the stock difference method, are recommended to quantify fine-scale depositional processes and their contribution to sedimentary carbon stocks. Moreover, the integration of biomarker analysis and eddy covariance flux measurements improves carbon budgeting by enhancing source identification and spatiotemporal flux monitoring. Among environmental factors, freshwater inflow is critical for increasing carbon sequestration efficiency by mitigating soil salinity, whereas frequent redox cycles triggered by tidal flat resuspension hinder organic carbon preservation. Although <em>Spartina alterniflora</em> enhances localized soil organic carbon (SOC) content, it does not significantly increase total SOC stocks and instead undermines ecosystem stability by displacing native species. The review also outlines degradation threats and recommends blue carbon management strategies, including legal restrictions on reclamation and trial implementation of carbon trading mechanisms. Overall, this work reinforces the strategic value of the Yellow River Delta in advancing sustainable coastal wetland management and global carbon neutrality targets.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106172"},"PeriodicalIF":4.1,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-27DOI: 10.1016/j.ibiod.2025.106181
Zhimin Xu , Zheng Lin , Runtong Huang , Xueqing Chen , Lei Wang , Xingying Deng , Rui Du , Jiguang Gu , Yifan Wang , Renqiang Yu
The black soldier flies (BSFs) are widely used for efficient conversion of livestock and poultry manure, but the impacts of microplastics (MPS) present in the manure on this process remain unclear. This study investigated the effects of 0.2 % (w/w) PVC microplastics (200 nm) on the bioconversion efficiency of manure by BSFs over a two-week larval development period, incorporating transcriptomic analysis to examine their impacts on larval growth, development, and metabolic processes. Results showed that MPS exposure altered manure physicochemical properties, making it wetter and less palatable, which reduced nitrogen transformation and inhibited larval growth by 11.05 % in body weight and 4.42 % in length. Meanwhile, oxidative stress was significantly elevated, with SOD activity increasing by 106 %, leading to a 26 % increase in early pupation. Transcriptomic analysis revealed that MPS accumulated in the BSF intestine disrupted glutamine metabolism, triggered inflammatory responses, and elevated reactive oxygen species production. Intestinal damage such as villus shedding induced the upregulation of serine endopeptidases. To compensate for impaired digestion, key genes involved in gluconeogenesis were significantly upregulated, including protein kinase A (2.39-fold), L-lactate dehydrogenase (1.72-fold), and pyruvate kinase (2.05-fold), helping to maintain energy homeostasis. Furthermore, nitrogen metabolism pathways showed enhanced expression of glutamate-related genes, facilitating the transition to the prepupal stage. These molecular and physiological responses represent an adaptive mechanism to environmental stress but ultimately resulted in compromised bioconversion efficiency. Therefore, removing MPS from manure is crucial for optimizing BSF-based bioconversion and promoting sustainable waste treatment.
{"title":"Microplastics impair black soldier fly bioconversion of pigeon manure: Physiological and transcriptomic insights","authors":"Zhimin Xu , Zheng Lin , Runtong Huang , Xueqing Chen , Lei Wang , Xingying Deng , Rui Du , Jiguang Gu , Yifan Wang , Renqiang Yu","doi":"10.1016/j.ibiod.2025.106181","DOIUrl":"10.1016/j.ibiod.2025.106181","url":null,"abstract":"<div><div>The black soldier flies (BSFs) are widely used for efficient conversion of livestock and poultry manure, but the impacts of microplastics (MPS) present in the manure on this process remain unclear. This study investigated the effects of 0.2 % (w/w) PVC microplastics (200 nm) on the bioconversion efficiency of manure by BSFs over a two-week larval development period, incorporating transcriptomic analysis to examine their impacts on larval growth, development, and metabolic processes. Results showed that MPS exposure altered manure physicochemical properties, making it wetter and less palatable, which reduced nitrogen transformation and inhibited larval growth by 11.05 % in body weight and 4.42 % in length. Meanwhile, oxidative stress was significantly elevated, with SOD activity increasing by 106 %, leading to a 26 % increase in early pupation. Transcriptomic analysis revealed that MPS accumulated in the BSF intestine disrupted glutamine metabolism, triggered inflammatory responses, and elevated reactive oxygen species production. Intestinal damage such as villus shedding induced the upregulation of serine endopeptidases. To compensate for impaired digestion, key genes involved in gluconeogenesis were significantly upregulated, including protein <em>kinase A</em> (2.39-fold), <em>L-lactate dehydrogenase</em> (1.72-fold), and <em>pyruvate kinase</em> (2.05-fold), helping to maintain energy homeostasis. Furthermore, nitrogen metabolism pathways showed enhanced expression of glutamate-related genes, facilitating the transition to the prepupal stage. These molecular and physiological responses represent an adaptive mechanism to environmental stress but ultimately resulted in compromised bioconversion efficiency. Therefore, removing MPS from manure is crucial for optimizing BSF-based bioconversion and promoting sustainable waste treatment.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106181"},"PeriodicalIF":4.1,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-27DOI: 10.1016/j.ibiod.2025.106167
António Carrapiço , Manuel Pico , Elisabete P. Carreiro , Pedro Barrulas , José Mirão , Ana Teresa Caldeira , Jorge Teixeira , Luís Dias , Maria Rosario Martins
Synthesis and application of nanoparticles (NPs) have shown promising results in various scientific fields due to their differentiating properties. However, current nanoparticle synthesis methods present environmental challenges. In this study biosynthesized silver nanoparticles (AgNPs) were produced from microbial culture supernatants as an alternative to traditional routes for controlling biodeteriogenic microorganisms in cultural heritage materials. The particles strongly inhibited cultural heritage colonizing bacteria and fungi, with minimum inhibitory concentrations ranging from 3 to 28 ppm. Kinetic analysis of disc diffusion assays revealed that inhibition zones correlate directly with silver ion release rates, rather than nanoparticle diffusion in agar. This led to a proposition of a simplified mechanistic model of Ag-based NPs behavior in disc diffusion assay. These findings carry broad implications - not only for the conservation of Cultural Heritage but also for diverse sectors, particularly human health - where accurate assessment of antimicrobial efficacy is paramount. To ensure accurate assessments, alternative methods should be standardized.
{"title":"Biosynthesis of silver-based nanoparticles: Critical assessment of antimicrobial assays and diffusion kinetics analysis","authors":"António Carrapiço , Manuel Pico , Elisabete P. Carreiro , Pedro Barrulas , José Mirão , Ana Teresa Caldeira , Jorge Teixeira , Luís Dias , Maria Rosario Martins","doi":"10.1016/j.ibiod.2025.106167","DOIUrl":"10.1016/j.ibiod.2025.106167","url":null,"abstract":"<div><div>Synthesis and application of nanoparticles (NPs) have shown promising results in various scientific fields due to their differentiating properties. However, current nanoparticle synthesis methods present environmental challenges. In this study biosynthesized silver nanoparticles (AgNPs) were produced from microbial culture supernatants as an alternative to traditional routes for controlling biodeteriogenic microorganisms in cultural heritage materials. The particles strongly inhibited cultural heritage colonizing bacteria and fungi, with minimum inhibitory concentrations ranging from 3 to 28 ppm. Kinetic analysis of disc diffusion assays revealed that inhibition zones correlate directly with silver ion release rates, rather than nanoparticle diffusion in agar. This led to a proposition of a simplified mechanistic model of Ag-based NPs behavior in disc diffusion assay. These findings carry broad implications - not only for the conservation of Cultural Heritage but also for diverse sectors, particularly human health - where accurate assessment of antimicrobial efficacy is paramount. To ensure accurate assessments, alternative methods should be standardized.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106167"},"PeriodicalIF":4.1,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibiotics are extensively used in medicine, agriculture, aquaculture, and animal husbandry. Over recent decades, global consumption of both prescribed and unprescribed antibiotics has surged significantly. Due to poor absorption in living organisms, substantial amounts of antibiotics and their metabolites are excreted and enter wastewater systems. Additionally, effluents from healthcare facilities, agricultural runoff, and pharmaceutical industries contribute heavily to environmental antibiotic contamination. The presence of antibiotics in aquatic ecosystems poses serious risks to human health and aquatic life, even at nano- or microgram concentrations. This review offers a comprehensive analysis of antibiotic sources and their ecological impacts, including the dissemination of antibiotic resistance genes and the rise of multidrug-resistant bacteria. To better understand environmental persistence and interactions, the classification and mechanisms of action of major antibiotics are discussed. The review further explores advanced detection methods designed to quantify trace antibiotic levels, emphasizing approaches tailored to specific antibiotic classes. Among various remediation strategies, enzymatic bioremediation is highlighted as a highly promising solution. Enzymes such as β-lactamases, laccases, and peroxidases are evaluated for their efficacy in degrading diverse antibiotic classes. The role of microbial remediation involving bacteria and fungi in antibiotic degradation is also examined. By integrating insights into sources, impacts, detection, and remediation techniques, this review provides a holistic perspective on antibiotic pollution and outlines sustainable pathways to mitigate environmental and public health risks. Importantly, this study underscores enzymatic bioremediation's high specificity and potential for scalability, offering a critical roadmap for advancing sustainable antibiotic removal technologies.
{"title":"Combating antibiotic pollution and its impacts on the environment through sustainable remediation options: Current developments and challenges","authors":"Huma Fatima , Amrik Bhattacharya , Sarthak Gupta , Sunil Kumar Khare","doi":"10.1016/j.ibiod.2025.106166","DOIUrl":"10.1016/j.ibiod.2025.106166","url":null,"abstract":"<div><div>Antibiotics are extensively used in medicine, agriculture, aquaculture, and animal husbandry. Over recent decades, global consumption of both prescribed and unprescribed antibiotics has surged significantly. Due to poor absorption in living organisms, substantial amounts of antibiotics and their metabolites are excreted and enter wastewater systems. Additionally, effluents from healthcare facilities, agricultural runoff, and pharmaceutical industries contribute heavily to environmental antibiotic contamination. The presence of antibiotics in aquatic ecosystems poses serious risks to human health and aquatic life, even at nano- or microgram concentrations. This review offers a comprehensive analysis of antibiotic sources and their ecological impacts, including the dissemination of antibiotic resistance genes and the rise of multidrug-resistant bacteria. To better understand environmental persistence and interactions, the classification and mechanisms of action of major antibiotics are discussed. The review further explores advanced detection methods designed to quantify trace antibiotic levels, emphasizing approaches tailored to specific antibiotic classes. Among various remediation strategies, enzymatic bioremediation is highlighted as a highly promising solution. Enzymes such as β-lactamases, laccases, and peroxidases are evaluated for their efficacy in degrading diverse antibiotic classes. The role of microbial remediation involving bacteria and fungi in antibiotic degradation is also examined. By integrating insights into sources, impacts, detection, and remediation techniques, this review provides a holistic perspective on antibiotic pollution and outlines sustainable pathways to mitigate environmental and public health risks. Importantly, this study underscores enzymatic bioremediation's high specificity and potential for scalability, offering a critical roadmap for advancing sustainable antibiotic removal technologies.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106166"},"PeriodicalIF":4.1,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-27DOI: 10.1016/j.ibiod.2025.106171
Anjali Chaudhary, Sana Bano, Ram Chandra
The effluent from kraft paper mills is a significant cause of aquatic pollution owing to the discharge of substantial quantities of contaminated water. The study revealed that wastewater sludge discharged from kraft paper mill has different pollution parameters beyond their permissible limit i.e. phenol (350.21 μg/g), chlorophenol (437.507 μg/g), lignin (1197.512 μg/g), sodium (7164.371 μg/g), chloride (5641.087 μg/g), phosphate (610.621 μg/g), sulphate (5005.944 μg/g), heavy metals Ni (70,123 μg/g), Mn (22.903 μg/g), Cu (62.921 μg/g), Fe (182.256 μg/g), Pb (1.812 μg/g) and Cr (1.801 μg/g). GCMS analysis showed the presence of 3-Ethyltridecane (RT 37.57), 1,2-Pentanediol (RT 16.66), and (Z)-4-methyl-3-decen-2-ol (RT 42.25), 8-Methylnonanoic acid (RT 16.73), Octadecanoic acid ethenyl ester (RT 37.63), and 2-Octen-2-ol acetate (RT 42.35) in upstream and downstream sludge respectively. The detected pollutants are known for their toxicity and EDC properties which significantly cause adverse effect on aquatic organism. Metagenomic analysis showed Treponema & Azotobacter followed by Megasphaera & Prevotella and Pseudomonas & Azotobacter followed by Treponema & Georgenia in the upstream and downstream sludge respectively. Abundance of polymyxin resistance protein in sludge indicated a potential environmental threat. The toxicity test with Tubifex tubifex showed EC50 at 20 % concentration of both the sludge while mortality was noted at 80 % concentration of upstream sludge and 40 % concentration of downstream sludge. Seed germination test of Vigna radiata with sludge inhibited the germination at 20 % concentration of sludge of both site samples. The presented study provides important insights into contaminant features and bacterial communities in kraft paper mill waste contaminated environments.
{"title":"Deciphering the microbial and chemical complexity of kraft paper industry sludge through metagenomic and toxicological evaluation","authors":"Anjali Chaudhary, Sana Bano, Ram Chandra","doi":"10.1016/j.ibiod.2025.106171","DOIUrl":"10.1016/j.ibiod.2025.106171","url":null,"abstract":"<div><div>The effluent from kraft paper mills is a significant cause of aquatic pollution owing to the discharge of substantial quantities of contaminated water. The study revealed that wastewater sludge discharged from kraft paper mill has different pollution parameters beyond their permissible limit i.e. phenol (350.21 μg/g), chlorophenol (437.507 μg/g), lignin (1197.512 μg/g), sodium (7164.371 μg/g), chloride (5641.087 μg/g), phosphate (610.621 μg/g), sulphate (5005.944 μg/g), heavy metals Ni (70,123 μg/g), Mn (22.903 μg/g), Cu (62.921 μg/g), Fe (182.256 μg/g), Pb (1.812 μg/g) and Cr (1.801 μg/g). GCMS analysis showed the presence of 3-Ethyltridecane (RT 37.57), 1,2-Pentanediol (RT 16.66), and (Z)-4-methyl-3-decen-2-ol (RT 42.25), 8-Methylnonanoic acid (RT 16.73), Octadecanoic acid ethenyl ester (RT 37.63), and 2-Octen-2-ol acetate (RT 42.35) in upstream and downstream sludge respectively. The detected pollutants are known for their toxicity and EDC properties which significantly cause adverse effect on aquatic organism. Metagenomic analysis showed <em>Treponema</em> & <em>Azotobacter</em> followed by <em>Megasphaera</em> & <em>Prevotella</em> and <em>Pseudomonas</em> & <em>Azotobacter</em> followed by <em>Treponema</em> & <em>Georgenia</em> in the upstream and downstream sludge respectively. Abundance of polymyxin resistance protein in sludge indicated a potential environmental threat. The toxicity test with <em>Tubifex tubifex</em> showed EC50 at 20 % concentration of both the sludge while mortality was noted at 80 % concentration of upstream sludge and 40 % concentration of downstream sludge. Seed germination test of <em>Vigna radiata</em> with sludge inhibited the germination at 20 % concentration of sludge of both site samples. The presented study provides important insights into contaminant features and bacterial communities in kraft paper mill waste contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106171"},"PeriodicalIF":4.1,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-27DOI: 10.1016/j.ibiod.2025.106168
Rule Zhao , Yu Tao , Zhibo Shen , Hongli Huang , Yichun Zhu , Zheng Gong , Anwei Chen , Yaoyu Zhou , Xin Li
The bioremediation of heavy metal contaminated soil has attracted significant scientific interest, though high metal concentrations inhibit microbial activity and viability. Biochar, a carbon-rich material, offers an ideal carrier for microbial immobilization due to its favorable physicochemical properties. In this study, corn straw biochar was produced at pyrolysis temperatures of 400 °C, 600 °C, and 800 °C, and two cadmium(Cd)-tolerant bacterial strains (Achromobacter xylosoxidans and Enterobacter asburiae) were selected for Cd remediation. After confirming that the composite bacteria exhibited greater Cd resistance than individual strains, the microbes were immobilized onto the biochar. The bacteria-loaded biochar was applied to Cd-contaminated soil at dosages of 0.5 %, 1 %, and 2 % (w/w), followed by a 56-day incubation. Results demonstrated that bacteria-loaded biochar enhanced Cd immobilization in the soil, with a 2 % dosage decreasing DTPA-extractable Cd by 18.2 %–38.7 %. Sequential extraction analysis revealed the substantial changes in the distribution of Cd. At the pyrolysis temperature of 600 °C and a 2 % application dosage, the content of exchangeable Cd decreased by 9.3 %, while organic matter-bound and residual fraction increased by 11.3 %. Furthermore, the bacteria-loaded biochar treatment improved soil microbial activity and metabolic capacity, increased microbial diversity, and significantly enriched the relative abundance of Cd-stabilizing microbial communities.
{"title":"Effects of bacteria-loaded biochar at different pyrolysis temperatures on the immobilization of cadmium in soil","authors":"Rule Zhao , Yu Tao , Zhibo Shen , Hongli Huang , Yichun Zhu , Zheng Gong , Anwei Chen , Yaoyu Zhou , Xin Li","doi":"10.1016/j.ibiod.2025.106168","DOIUrl":"10.1016/j.ibiod.2025.106168","url":null,"abstract":"<div><div>The bioremediation of heavy metal contaminated soil has attracted significant scientific interest, though high metal concentrations inhibit microbial activity and viability. Biochar, a carbon-rich material, offers an ideal carrier for microbial immobilization due to its favorable physicochemical properties. In this study, corn straw biochar was produced at pyrolysis temperatures of 400 °C, 600 °C, and 800 °C, and two cadmium(Cd)-tolerant bacterial strains (<em>Achromobacter xylosoxidans</em> and <em>Enterobacter asburiae</em>) were selected for Cd remediation. After confirming that the composite bacteria exhibited greater Cd resistance than individual strains, the microbes were immobilized onto the biochar. The bacteria-loaded biochar was applied to Cd-contaminated soil at dosages of 0.5 %, 1 %, and 2 % (w/w), followed by a 56-day incubation. Results demonstrated that bacteria-loaded biochar enhanced Cd immobilization in the soil, with a 2 % dosage decreasing DTPA-extractable Cd by 18.2 %–38.7 %. Sequential extraction analysis revealed the substantial changes in the distribution of Cd. At the pyrolysis temperature of 600 °C and a 2 % application dosage, the content of exchangeable Cd decreased by 9.3 %, while organic matter-bound and residual fraction increased by 11.3 %. Furthermore, the bacteria-loaded biochar treatment improved soil microbial activity and metabolic capacity, increased microbial diversity, and significantly enriched the relative abundance of Cd-stabilizing microbial communities.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106168"},"PeriodicalIF":4.1,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-23DOI: 10.1016/j.ibiod.2025.106163
Xiao Hui , Zulhelmi Amir , Mohd Usman Mohd Junaidi , Fathiah Mohamed Zuki , Xuecheng Zheng
The residual Partially Hydrolyzed Polyacrylamide (HPAM) and the crosslinker (Cr3+) after polymer gel formation caused blockages and decreased recovery. Microorganisms can degrade HPAM to address the issues, but Cr3+ inhibits the process and consequently reduces oil recovery. This study investigates an underexplored area—the biodegradation of HPAM in the presence of Cr3+. HPAM and crude oil were innovatively used as the sole nitrogen(N) and carbon(C) sources, respectively, to isolate the highest-growing strain from Daqing Oilfield production water, identified as Bacillus licheniformis SP01. Fourier transform infrared spectroscopy, high-performance liquid chromatography and scanning electron microscopy analyses revealed Cr3+-induced structural and morphological changes in HPAM after biodegradation. B. licheniformis SP01 exhibited a maximum HPAM degradation rate of 39.66 %, which declined to 32.41 % (a 7.25 % decrease) upon exposure to Cr3+. This was accompanied by a 43.9 % drop in biomass and declines in amidase and urease activity by 35.2 % and 19.5 %, respectively, implying that Cr3+ impairs microbial growth and enzymatic activities, thereby reducing HPAM biodegradation. Mass balance and stoichiometry investigations of C and N in HPAM indicate that Cr3+ alters both the biodegradation pathway and final degradation products. Furthermore, this study explores the impact of reservoir conditions on biodegradation with residual Cr3+ present and predict the optimal degradation rate of 37.10 %. These results highlight the inhibitory effects of Cr3+ on HPAM biodegradation mechanism and propose a bioremediation strategy to mitigate reservoir plugging, improving oil recovery after polymer gel formation.
{"title":"Residual Cr3+ on the biodegradation of partially hydrolyzed polyacrylamide after polymer gel formation by Bacillus licheniformis SP01","authors":"Xiao Hui , Zulhelmi Amir , Mohd Usman Mohd Junaidi , Fathiah Mohamed Zuki , Xuecheng Zheng","doi":"10.1016/j.ibiod.2025.106163","DOIUrl":"10.1016/j.ibiod.2025.106163","url":null,"abstract":"<div><div>The residual Partially Hydrolyzed Polyacrylamide (HPAM) and the crosslinker (Cr<sup>3+</sup>) after polymer gel formation caused blockages and decreased recovery. Microorganisms can degrade HPAM to address the issues, but Cr<sup>3+</sup> inhibits the process and consequently reduces oil recovery. This study investigates an underexplored area—the biodegradation of HPAM in the presence of Cr<sup>3+</sup>. HPAM and crude oil were innovatively used as the sole nitrogen(N) and carbon(C) sources, respectively, to isolate the highest-growing strain from Daqing Oilfield production water, identified as <em>Bacillus licheniformis</em> SP01. Fourier transform infrared spectroscopy, high-performance liquid chromatography and scanning electron microscopy analyses revealed Cr<sup>3+</sup>-induced structural and morphological changes in HPAM after biodegradation. <em>B. licheniformis</em> SP01 exhibited a maximum HPAM degradation rate of 39.66 %, which declined to 32.41 % (a 7.25 % decrease) upon exposure to Cr<sup>3+</sup>. This was accompanied by a 43.9 % drop in biomass and declines in amidase and urease activity by 35.2 % and 19.5 %, respectively, implying that Cr<sup>3+</sup> impairs microbial growth and enzymatic activities, thereby reducing HPAM biodegradation. Mass balance and stoichiometry investigations of C and N in HPAM indicate that Cr<sup>3+</sup> alters both the biodegradation pathway and final degradation products. Furthermore, this study explores the impact of reservoir conditions on biodegradation with residual Cr<sup>3+</sup> present and predict the optimal degradation rate of 37.10 %. These results highlight the inhibitory effects of Cr<sup>3+</sup> on HPAM biodegradation mechanism and propose a bioremediation strategy to mitigate reservoir plugging, improving oil recovery after polymer gel formation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"205 ","pages":"Article 106163"},"PeriodicalIF":4.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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