{"title":"Tubule Clustered Body Accompanied with Glycogen Particles in the Striated Duct Cell of Submandibular Gland of the Volcano Rabbit, Romerolagus diaz","authors":"T. Mineda","doi":"10.2330/JORALBIOSCI1965.43.325","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.325","url":null,"abstract":"特異な小器官であるグリコーゲン顆粒を伴う細管集合小体が, しばしばメキシコウサギ顎下腺の線条部細胞に存在した.細管集合小体は滑面小胞体の集合体として生じ, ときどき短い管状の粗面小胞体と小体の周辺で連結するのが示された.ある断面の切片で, 小体のそれぞれの細管 (直径20-25nm) はリング状, 亜鈴状さらに湾曲した細管状の様相を示す.種々の小体像は, 平行配列した滑面小胞体の螺旋細管系の切断方向によるものである.細胞頂部の小さな小体は多量のグリコーゲン顆粒, 少しの脂肪滴を伴うが, 核下部における発達した小体はグリコーゲン顆粒を伴わない傾向がある.これらの結果は, グリコーゲン付随小体が細胞頂部から核下部細胞質に広く散在し, グリコーゲンおよび, または脂質の代謝に関与することが示唆される.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"3 1","pages":"325-333"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80830874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-06-20DOI: 10.2330/JORALBIOSCI1965.43.278
M. Hattori, Y. Taya, Kaori Sato, T. Aoba
To elucidate how the fluid environment in forming enamel is modified by ion transport through the ameloblasts and associated extracellular events, we studied precipitation reactions in an experimental model utilizing a dialysis chamber. The experimental set-up comprised of upper and lower compartments, which were separated by a dialysis membrane of various cut-off sizes. Synthetic hydroxyapatite and pig enamel proteins were used as seeds and adsorbates, respectively. Seed crystals with or without proteincoating were placed in the lower compartment. Upon feeding the supersaturated solution (containing 1mM or 0.5mM Ca) in the upper compartment, Ca ion was allowed to diffuse into the lower compartment according to the existing concentration gradients. By monitoring time-sequential changes in Ca concentration in the lower solution, we found that Ca supply through the membrane was a rate-determining step, so the Ca concentration in the medium surrounding seed crystals became in proximity to the steady-state condition, under which the Ca concentration ([Ca] s) was maintained at lower levels than that of the supersaturated solution filling the upper compartment. The establishment of [Ca] s in the mineralizing milieu was speculated to be due to the kinetic difference between Ca supply and consumption for precipitation because [Ca] s levels were affected markedly by the presence of regulators (i. e., enamel proteins and fluoride) for precipitation. Notably, fluoride at 1ppm or lower levels exerted decreasing effects on [Ca] s, due to accelerating the precipitation kinetics and lowering the solubility of precipitated fluoridated crystals. The overall results obtained support the importance of the multitudinous physico-chemical processes in determin- ing the fluid composition surrounding forming enamel mineral.
{"title":"Kinetic Consideration of Ca Transport and Associated Crystal Precipitation in an in vitro Precipitation Model Using Dialysis Membranes: Fluoride-dependent Regulation of Ca Concentration in Liquid Environment","authors":"M. Hattori, Y. Taya, Kaori Sato, T. Aoba","doi":"10.2330/JORALBIOSCI1965.43.278","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.278","url":null,"abstract":"To elucidate how the fluid environment in forming enamel is modified by ion transport through the ameloblasts and associated extracellular events, we studied precipitation reactions in an experimental model utilizing a dialysis chamber. The experimental set-up comprised of upper and lower compartments, which were separated by a dialysis membrane of various cut-off sizes. Synthetic hydroxyapatite and pig enamel proteins were used as seeds and adsorbates, respectively. Seed crystals with or without proteincoating were placed in the lower compartment. Upon feeding the supersaturated solution (containing 1mM or 0.5mM Ca) in the upper compartment, Ca ion was allowed to diffuse into the lower compartment according to the existing concentration gradients. By monitoring time-sequential changes in Ca concentration in the lower solution, we found that Ca supply through the membrane was a rate-determining step, so the Ca concentration in the medium surrounding seed crystals became in proximity to the steady-state condition, under which the Ca concentration ([Ca] s) was maintained at lower levels than that of the supersaturated solution filling the upper compartment. The establishment of [Ca] s in the mineralizing milieu was speculated to be due to the kinetic difference between Ca supply and consumption for precipitation because [Ca] s levels were affected markedly by the presence of regulators (i. e., enamel proteins and fluoride) for precipitation. Notably, fluoride at 1ppm or lower levels exerted decreasing effects on [Ca] s, due to accelerating the precipitation kinetics and lowering the solubility of precipitated fluoridated crystals. The overall results obtained support the importance of the multitudinous physico-chemical processes in determin- ing the fluid composition surrounding forming enamel mineral.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"22 1","pages":"278-291"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83941708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-06-20DOI: 10.2330/JORALBIOSCI1965.43.268
Yoshimasa Imaminato, K. Ishizuka, T. Murakami
The aim of this study was to clarify the role of spinal trigeminal subnucleus interporalis (Sp 5 I) and subnucleus caudalis (Sp 5 C) on the trigeminal-salivary reflex. Extracellular recordings were made from a total of 119 superior salivatory nucleus (SSN) neurons innervating the submandibular and sublingual glands in urethane-chloralose anaesthetized cats. Responses to stimulation of the inferior alveolar (IA) nerve were investigated in trigeminal tractotomized animals at the level of the obex (Sp 5 C animals) and at the level of 2.5-3mm rostral from the obex (Sp 5 I animals) as well as in non-tractotomized (control) animals. In control, Sp 5 C and Sp 5 I animals, 50% (23/46), 46% (15/33) and 10% (4/40) of the identified SSN neurons orthodromically responded to stimulation of the IA nerve, respectively.In Sp 5 C animals, no significant reduction of the response rate was found in comparison with control animals, and there was no significant difference in the mean onset latency between control and Sp 5 C animals. However, only the responses activated by the medium-threshold afferent fibres or by high-threshold afferent fibres could be observed.In Sp 5 I animals, a marked reduction of the response rate was noted in comparison with control animals, and there was no significant difference in the mean onset latency between control and Sp 5 I animals. However, only the responses excited by the medium-threshold afferent fibres or by high-threshold afferent fibres could be observed.As the relay nucleus in the trigeminal-salivary reflex, Sp 5 I has a more important role and Sp 5 C has a lesser role.
{"title":"Analysis of the Central Pathway of the Trigeminal-Salivary Reflex by the Spinal Trigeminal Tractotomy","authors":"Yoshimasa Imaminato, K. Ishizuka, T. Murakami","doi":"10.2330/JORALBIOSCI1965.43.268","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.268","url":null,"abstract":"The aim of this study was to clarify the role of spinal trigeminal subnucleus interporalis (Sp 5 I) and subnucleus caudalis (Sp 5 C) on the trigeminal-salivary reflex. Extracellular recordings were made from a total of 119 superior salivatory nucleus (SSN) neurons innervating the submandibular and sublingual glands in urethane-chloralose anaesthetized cats. Responses to stimulation of the inferior alveolar (IA) nerve were investigated in trigeminal tractotomized animals at the level of the obex (Sp 5 C animals) and at the level of 2.5-3mm rostral from the obex (Sp 5 I animals) as well as in non-tractotomized (control) animals. In control, Sp 5 C and Sp 5 I animals, 50% (23/46), 46% (15/33) and 10% (4/40) of the identified SSN neurons orthodromically responded to stimulation of the IA nerve, respectively.In Sp 5 C animals, no significant reduction of the response rate was found in comparison with control animals, and there was no significant difference in the mean onset latency between control and Sp 5 C animals. However, only the responses activated by the medium-threshold afferent fibres or by high-threshold afferent fibres could be observed.In Sp 5 I animals, a marked reduction of the response rate was noted in comparison with control animals, and there was no significant difference in the mean onset latency between control and Sp 5 I animals. However, only the responses excited by the medium-threshold afferent fibres or by high-threshold afferent fibres could be observed.As the relay nucleus in the trigeminal-salivary reflex, Sp 5 I has a more important role and Sp 5 C has a lesser role.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"137 1","pages":"268-277"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89765917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-06-20DOI: 10.2330/JORALBIOSCI1965.43.316
M. Matsuo, Takatsuna Nakamura, Kazuto Takahashi
In this study we investigated vascular regeneration and bone formation in regard to the morphological view and image analysis.The mandibular molars of beagle dogs were extracted, and healing was allowed for 90 days. A sandblasted and acid-etched (SLA) implant was inserted on one side and as a control, a titanium plasma-splayed (TPS) implant was inserted on the contralateral side. Vascular resin injection was given at 3 and 6 weeks after the implantation. Specimens were freeze dried, and observed by scanning electron microscope (SEM).At 3 weeks after the implantation, the bone surface of the SLA implant was relatively smooth and there were some osseous lacunae. In contrast, rough, porous bone tissue was formed in the TPS implant and the bone surface had more lacunae than the SLA implant. At 6 weeks vessels in the osseous lacunae on the bone surface of the SLA implants, were reduced, while new bone formation was not completed around the TPS implants. On image analysis, a higher bone contact ratio was noted in the SLA group (54.7% 3W, 70.8% at 6W) than the TPS group (48.3% 3W, 60.6% at 6W).These result suggests that a surface treatment for implants can accelerate the regeneration of perimplant bone and blood vessels.
{"title":"Bone Formation and Microvascular Changes after Placement of Sandblasted and Acid-etched (SLA) and Titanium Plasma-splayed (TPS) Implants in Dogs","authors":"M. Matsuo, Takatsuna Nakamura, Kazuto Takahashi","doi":"10.2330/JORALBIOSCI1965.43.316","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.316","url":null,"abstract":"In this study we investigated vascular regeneration and bone formation in regard to the morphological view and image analysis.The mandibular molars of beagle dogs were extracted, and healing was allowed for 90 days. A sandblasted and acid-etched (SLA) implant was inserted on one side and as a control, a titanium plasma-splayed (TPS) implant was inserted on the contralateral side. Vascular resin injection was given at 3 and 6 weeks after the implantation. Specimens were freeze dried, and observed by scanning electron microscope (SEM).At 3 weeks after the implantation, the bone surface of the SLA implant was relatively smooth and there were some osseous lacunae. In contrast, rough, porous bone tissue was formed in the TPS implant and the bone surface had more lacunae than the SLA implant. At 6 weeks vessels in the osseous lacunae on the bone surface of the SLA implants, were reduced, while new bone formation was not completed around the TPS implants. On image analysis, a higher bone contact ratio was noted in the SLA group (54.7% 3W, 70.8% at 6W) than the TPS group (48.3% 3W, 60.6% at 6W).These result suggests that a surface treatment for implants can accelerate the regeneration of perimplant bone and blood vessels.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"3 1","pages":"316-324"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77983763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-06-20DOI: 10.2330/JORALBIOSCI1965.43.229
Y. Kudo
{"title":"Expression of p27Kip1 and Its Roles in Oral Squamous Cell Carcinoma","authors":"Y. Kudo","doi":"10.2330/JORALBIOSCI1965.43.229","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.229","url":null,"abstract":"","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"5 1 1","pages":"229-238"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78328517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-06-20DOI: 10.2330/JORALBIOSCI1965.43.239
K. Takeda
{"title":"Regulation of Cell Survival, Apoptosis and Differentiation by ASK 1","authors":"K. Takeda","doi":"10.2330/JORALBIOSCI1965.43.239","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.239","url":null,"abstract":"","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"17 1","pages":"239-246"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91030494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-06-20DOI: 10.2330/JORALBIOSCI1965.43.310
C. Iwai, S. Tsurusako, Y. Ohara, M. Obuchi, N. Segami
To elucidate the pathogenesis of dental focal infection associated with skin lesion, serological and bacteriological studies in 52 patients with various dermatoses were performed. None of the 52 patients were accompanied with tonsillar or sinus lesions, but they all had chronic dental lesions. The isolation of causative microbes and the evaluation of serum anti-streptolysin O (ASO), anti-streptokinase (ASK), anti-deoxyribonuclease B (ADN-B) were performed. Alpha-hemolytic cocci were most frequently isolated and ASO, ASK, and ADN-B values, which are the inarkers for β-hemolytic streptococcal infection, were negative in many cases. In patients in whom α-hemolytic streptococci were isolated, agglutinin titers were examined and we found that these patients generally had high agglutination titers against the isolated α-hemolytic streptococci. Some humoral immunological reactions to bacterial surface determinants may play a role in the pathogenesis.
{"title":"Dental Focal Infection Associated with Skin Diseases","authors":"C. Iwai, S. Tsurusako, Y. Ohara, M. Obuchi, N. Segami","doi":"10.2330/JORALBIOSCI1965.43.310","DOIUrl":"https://doi.org/10.2330/JORALBIOSCI1965.43.310","url":null,"abstract":"To elucidate the pathogenesis of dental focal infection associated with skin lesion, serological and bacteriological studies in 52 patients with various dermatoses were performed. None of the 52 patients were accompanied with tonsillar or sinus lesions, but they all had chronic dental lesions. The isolation of causative microbes and the evaluation of serum anti-streptolysin O (ASO), anti-streptokinase (ASK), anti-deoxyribonuclease B (ADN-B) were performed. Alpha-hemolytic cocci were most frequently isolated and ASO, ASK, and ADN-B values, which are the inarkers for β-hemolytic streptococcal infection, were negative in many cases. In patients in whom α-hemolytic streptococci were isolated, agglutinin titers were examined and we found that these patients generally had high agglutination titers against the isolated α-hemolytic streptococci. Some humoral immunological reactions to bacterial surface determinants may play a role in the pathogenesis.","PeriodicalId":14631,"journal":{"name":"Japanese Journal of Oral Biology","volume":"22 1","pages":"310-315"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89394180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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