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Analysis of Carbon Flux Characteristics in Saline–Alkali Soil Under Global Warming 全球变暖条件下盐碱地的碳通量特征分析
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-01 DOI: 10.1111/jac.12720
Qiu Haonan, Yang Shihong, Wang Guangmei, Liu Xiaoling, Zhang Jie, Xu Yi, Dong Shide, Liu Hanwen, Jiang Zewei

The carbon cycle of saline–alkali ecosystems will be affected to some extent in the context of future global warming. Therefore, we investigated the net ecosystem exchange (NEE) of three typical crops (wheat, maize and soybean) in the saline–alkaline land of the Yellow River Delta. To further investigate CO2 fluxes, NEE was decomposed into gross primary productivity (GPP) and ecosystem respiration (Re). In terms of seasonal variation, wheat and soybean were carbon sources in the early and late growth periods, and carbon sinks in the rest of the period, whereas maize was a carbon sink in the majority of the period, and maize had good carbon sink potential. The cumulative NEE during the growth periods for wheat, maize, and soybean were 414.86, 258.24 and 228.92 g cm−2, respectively, and the daily variation showed that the peak NEE values for the three crops preceded the peak values of both GPP and ecosystem respiration, occurring approximately at 12:00 a.m. In the correlation analysis, NEE and GPP of the three crops were well correlated with photosynthetic photon flux density and net radiation, whereas Re was significantly correlated with air temperature. Through a comparative analysis of CO2 fluxes within various agricultural ecosystems, our findings indicated that wheat demonstrated moderate carbon sequestration capabilities, whereas maize and soybean exhibited strong carbon sink characteristics. Notably, saline–alkali crops exhibited lower Re, whereas GPP levels remained at a moderate range. Therefore, under the global warming trend, the respiration of saline crops and soils will be affected and may change the original carbon sink into a carbon source. Hence, implementing suitable measures targeting saline–alkali areas, such as the establishment of an effective crop rotation system and the enhance saline–alkali land conditions, can reduce emissions of greenhouse gases, thus reducing the pressure of global warming and maintaining a stable carbon cycle in saline–alkali land.

在未来全球变暖的背景下,盐碱地生态系统的碳循环将受到一定程度的影响。因此,我们研究了黄河三角洲盐碱地中三种典型作物(小麦、玉米和大豆)的净生态系统交换(NEE)。为了进一步研究二氧化碳通量,我们将 NEE 分解为总初级生产力(GPP)和生态系统呼吸作用(Re)。从季节变化来看,小麦和大豆在生长前期和后期为碳源,其余时间为碳汇,而玉米在大部分时间为碳汇,玉米具有良好的碳汇潜力。小麦、玉米和大豆在生长期的累积NEE分别为414.86、258.24和228.92 g cm-2,从日变化来看,三种作物的NEE峰值先于GPP和生态系统呼吸的峰值,大约出现在上午12:00。通过对不同农业生态系统中二氧化碳通量的比较分析,我们的研究结果表明,小麦具有中等的固碳能力,而玉米和大豆则表现出较强的碳汇特征。值得注意的是,盐碱地作物的 Re 值较低,而 GPP 水平则保持在中等范围。因此,在全球变暖的趋势下,盐碱地作物和土壤的呼吸作用将受到影响,并可能将原来的碳汇转变为碳源。因此,针对盐碱地采取适当的措施,如建立有效的轮作制度、改善盐碱地条件等,可以减少温室气体的排放,从而减轻全球变暖的压力,维持盐碱地碳循环的稳定。
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引用次数: 0
Multigenerational Effects of Elevated CO2 and N Supply on Leaf Gas Exchange Traits in Wheat Plants 高浓度二氧化碳和氮供应对小麦叶片气体交换性状的多代效应
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-07-01 DOI: 10.1111/jac.12722
Xizi Wang, Eva Rosenqvist, Yuzheng Zong, Xiangnan Li, Fulai Liu

The responses of leaf gas exchange of wheat (Triticum aestivum L.) to elevated atmospheric CO2 concentration (e[CO2]) were often investigated within a single generation, while the long-term acclimation of photosynthesis to growth in e[CO2] over multiple generations has not been systematically studied. Here, five wheat cultivars were grown under either ambient (a[CO2], 400 ppm) or elevated (e[CO2], 800 ppm) CO2 concentration for three consecutive generations (G1 to G3) with two N-fertilisation levels (1N–1 g N pot−1 and 2N–2 g N pot−1) in climate-controlled greenhouses. Leaf gas exchange was determined in each generation of plants under different treatments. It was found that at both N levels, e[CO2] stimulated photosynthetic rate while reducing stomatal conductance, transpiration rate and leaf N concentration, resulting in an enhanced water use efficiency and photosynthetic N use efficiency. The N level modulated the intergenerational responses of photosynthetic capacity to e[CO2]; under low N supply, the maximum carboxylation rate (Vcmax), the maximum electron transport rate (Jmax) and the rate of triose phosphate utilisation (TPU) were significantly downregulated by e[CO2] from the first to the second generation, but recovered in the third generation; whereas at high N levels, photosynthetic acclimation was diminished with the progress of generations, with Vcmax, Jmax and TPU increased under e[CO2] in the third generation. These results suggest that intergenerational adaptation could alleviate the e[CO2]-induced reduction of the photosynthetic capacity, but plants with different N status responded differently to adapt to the long-term exposure to e[CO2]. Among the five cultivars, 325Jimai showed a better photosynthetic performance under e[CO2] over the three generations, while 02-1Shiluan appeared to be more inhibited by CO2 elevation in the long term conditions. These findings provide new insights for breeding strategies in the future CO2-enriched environments.

小麦(Triticum aestivum L.)叶片气体交换对大气二氧化碳浓度(e[CO2])升高的响应通常在单代内进行研究,而光合作用对多代 e[CO2] 生长的长期适应性尚未得到系统研究。在此,五个小麦栽培品种在环境(a[CO2],400 ppm)或高浓度(e[CO2],800 ppm)CO2 条件下连续生长了三代(G1 至 G3),并在气候控制温室中施用了两种氮肥水平(1N-1 g N pot-1 和 2N-2 g N pot-1)。在不同处理条件下测定了每一代植物的叶片气体交换量。结果发现,在两种氮水平下,e[CO2]都能刺激光合速率,同时降低气孔导度、蒸腾速率和叶片氮浓度,从而提高水分利用效率和光合作用氮利用效率。氮水平调节了光合作用能力对 e[CO2] 的代际响应;在低氮供应条件下,e[CO2]显著降低了第一代到第二代光合作用的最大羧化速率(Vcmax)、最大电子传输速率(Jmax)和磷酸三糖利用率(TPU),但在第三代又有所恢复;而在高氮量条件下,光合作用的适应性随着世代的增加而减弱,第三代的 Vcmax、Jmax 和 TPU 在 e[CO2] 条件下有所增加。这些结果表明,代际适应可以缓解 e[CO2] 诱导的光合能力下降,但不同氮素状态的植物对长期暴露于 e[CO2] 的适应反应不同。在五个栽培品种中,325吉迈在三代e[CO2]条件下表现出较好的光合性能,而02-1世绿在长期条件下似乎更受二氧化碳升高的抑制。这些发现为未来二氧化碳富集环境下的育种策略提供了新的启示。
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引用次数: 0
Effects of Semi-Film and Full-Film Mulching on Soybean Growth, Biological Nitrogen Fixation and Grain Yield 半膜和全膜覆盖对大豆生长、生物固氮和谷物产量的影响
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-27 DOI: 10.1111/jac.12724
Shuyue Wen, Pengfei Dang, Dexiao Li, Xiaoliang Qin, Kadambot H. M. Siddique

Soybean film mulching has shown promise in maintaining consistent and high yields in semi-arid regions. However, the specific impacts of full-film and semi-film mulching on soybean growth, root nodule traits and grain yield are poorly understood. This 2-year study (2021–2022) investigates the effects of full-film and semi-film mulching on soil moisture, soybean growth and yield. Our findings revealed that semi-film mulching increased soybean yield by 18.12% compared to full-film mulching, averaged across 2 years. Furthermore, the semi-film treatment significantly enhanced biological nitrogen fixation ability, increasing root nodule numbers by 24.81%–33.43%, compared to full-film mulching. This improvement also positively affected soybean quality, with crude protein content increasing by 5.89% in 2021 and 4.14% in 2022 compared to full-filming. Moreover, semi-film mulching helped maintain soil moisture and temperature during later soybean growth stages. There findings suggest that semi-film mulching is a viable agricultural strategy for soybean cultivation in semi-arid regions, improving soybean quality and efficiency while promoting environmental sustainability.

在半干旱地区,大豆薄膜覆盖有望保持稳定高产。然而,人们对全膜和半膜覆盖对大豆生长、根瘤性状和谷物产量的具体影响知之甚少。这项为期两年(2021-2022 年)的研究调查了全膜和半膜覆盖对土壤水分、大豆生长和产量的影响。我们的研究结果表明,与全膜覆盖相比,半膜覆盖使大豆产量提高了 18.12%,两年的平均值为 18.12%。此外,与全膜覆盖相比,半膜覆盖显著提高了生物固氮能力,使根瘤数量增加了 24.81%-33.43% 。这种改善也对大豆质量产生了积极影响,与全膜覆盖相比,2021 年和 2022 年的粗蛋白含量分别增加了 5.89% 和 4.14%。此外,在大豆生长后期,半膜覆盖有助于保持土壤湿度和温度。研究结果表明,在半干旱地区种植大豆,半膜覆盖是一种可行的农业策略,既能提高大豆的质量和效率,又能促进环境的可持续发展。
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引用次数: 0
Severe Preharvest Drought Elevates Respiration and Storage Rot in Postharvest Sugarbeet Roots 收获前的严重干旱会提高收获后甜菜根部的呼吸作用和贮藏腐烂程度
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-25 DOI: 10.1111/jac.12718
Abbas M. Lafta, John D. Eide, Mohamed F. R. Khan, Fernando L. Finger, Karen K. Fugate

Sugarbeets are largely produced without irrigation, making drought stress inevitable when rainfall is insufficient. Whether drought stress impacts root storage, however, is currently unknown. Research was conducted to determine the effect of preharvest water stress on postharvest sugarbeet root respiration rate and susceptibility to storage rots as these traits are the primary determinants for sucrose loss and quality deterioration. Greenhouse-grown plants were subjected to four levels of water deficit by discontinuing watering for 0, 7, 14 or 21 days prior to harvest. Plants receiving water-restrictive treatments displayed physiological stress by leaf epinasty, reductions in net photosynthetic rate and leaf relative water content and increases in leaf temperature, whereas the water content of roots harvested from these plants progressively decreased with the severity of the preharvest water-deficit treatment. Harvested roots from all watering treatments were stored at 10°C and 95% relative humidity for up to 12 weeks and evaluated for respiration rate and susceptibility to storage rot. Root respiration rate during storage was inversely related to root water content at harvest by second-order equations, such that respiration was not significantly affected by minor reductions in root water content but increased exponentially for roots obtained from severely drought-stressed plants with water contents at harvest of ≤75%. Similarly, roots with water contents ≤75% had elevated levels of electrolyte leakage, a measure of cellular membrane damage, and were more susceptible to dehydration and fungal infection during storage. In separate experiments, roots harvested from water-stressed plants were inoculated with Botrytis cinerea or Penicillium vulpinum, two causal agents for storage rots. In these experiments, preharvest water stress quantitatively increased root rot and qualitatively altered symptoms of their infection. Overall, these results demonstrate that severe preharvest drought stress is likely to significantly increase sugarbeet root storage losses caused by root respiration and storage rots and that storage losses are likely to accelerate with time in storage. However, mild-to-moderate drought conditions prior to harvest are expected to have no or minimal effect on storage losses from root respiration or storage rots.

甜菜的生产基本上不需要灌溉,因此当降雨量不足时,干旱胁迫不可避免。然而,干旱胁迫是否会影响根系的贮藏,目前还不得而知。研究旨在确定收获前水分胁迫对收获后甜菜根部呼吸速率和贮藏腐烂易感性的影响,因为这些性状是蔗糖损失和质量下降的主要决定因素。通过在收获前 0、7、14 或 21 天停止浇水,对温室种植的植物施加四种程度的水分亏缺。接受限水处理的植株表现出生理应激反应,叶片脱落,净光合速率和叶片相对含水量降低,叶温升高,而从这些植株上收获的根的含水量随着收获前缺水处理的严重程度而逐渐降低。在 10°C、相对湿度 95% 的条件下,将所有浇水处理下收获的根储存长达 12 周,并评估其呼吸速率和对贮藏腐烂的敏感性。根系在贮藏期间的呼吸速率与根系收获时的含水量呈二阶反比关系,因此根系含水量的轻微降低对呼吸速率的影响不大,但对于收获时含水量≤75%的严重干旱胁迫植株的根系,呼吸速率呈指数增长。同样,含水量≤75% 的根的电解质渗漏水平升高,这是细胞膜损伤的一个指标,而且在贮藏期间更容易脱水和受到真菌感染。在不同的实验中,从水分胁迫植物上收获的根部接种了灰霉病菌或青霉,这是两种导致贮藏腐烂的病原菌。在这些实验中,收获前的水分胁迫在量上增加了根腐病的发生,在质上改变了其感染症状。总之,这些结果表明,严重的收获前干旱胁迫可能会显著增加甜菜根部因根部呼吸和贮藏腐烂而造成的贮藏损失,而且贮藏损失可能会随着贮藏时间的延长而加快。不过,收获前的轻度至中度干旱条件预计不会对根呼吸或贮藏腐烂造成的贮藏损失产生影响或影响极小。
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引用次数: 0
Diurnal and Seasonal Variations of Water Use Efficiency of Rice–Wheat Rotation Cropland in the Jianghuai River Basin of China 中国江淮流域稻麦轮作耕地水分利用效率的日变化和季节变化
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-25 DOI: 10.1111/jac.12719
Xiaohan Zhao, Fangmin Zhang, Shengheng Weng, Chunfeng Duan, Yanyu Lu

Rice–wheat rotation cropland is one of the most important agroecosystems in South China, the escalation of conflict between food demand augment and water supply shortage increased with climate change. Water use efficiency plays a more significant role in optimising water and carbon management. Thus, the diurnal and seasonal variations of water use efficiency were assessed by the 3-year eddy covariance observations in the Shouxian National Observatory, a typical rice–wheat rotation station. The results revealed a ‘U’-shaped diurnal pattern of water use efficiency for winter wheat (Triticum aestivum L.) and rice (Oryza sativa L.). Seasonal water use efficiency had two peaks with the highest in the winter wheat-growing season. The average water use efficiency for the rice–wheat rotation cropland was 2.85 g C kg−1 H2O over the whole year with 2.62 and 3.11 g C kg−1 H2O for winter wheat and rice, respectively. However, gross primary productivity and evapotranspiration of rice were higher than those of winter wheat. Temperature, photosynthetically active radiation were the principal impact factors of water use efficiency in the rice-growing season. Comparatively, soil water and vapour pressure deficit dominated the water use efficiency changes in the winter wheat-growing season. Our analyses can help understand the water use requirements for carbon assimilation on rice–wheat rotation cropland on the field scale.

水稻-小麦轮作耕地是中国南方最重要的农业生态系统之一,随着气候变化,粮食需求增加与水资源供应短缺之间的矛盾日益加剧。用水效率在优化水与碳管理方面发挥着更重要的作用。因此,通过在寿县国家观测站(一个典型的稻麦轮作站)进行为期 3 年的涡度协方差观测,评估了用水效率的昼夜和季节变化。结果表明,冬小麦和水稻的水分利用效率呈 "U "形昼夜变化规律。季节用水效率有两个峰值,其中冬小麦生长季节用水效率最高。稻麦轮作耕地的全年平均用水效率为 2.85 g C kg-1 H2O,冬小麦和水稻分别为 2.62 和 3.11 g C kg-1 H2O。不过,水稻的总初级生产力和蒸散量均高于冬小麦。温度和光合有效辐射是水稻生长期水分利用效率的主要影响因素。相对而言,土壤水分和蒸汽压力亏缺主导了冬小麦生长期水分利用效率的变化。我们的分析有助于了解稻麦轮作耕地田间碳同化对水分的需求。
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引用次数: 0
Heat Stress Resulting From Late Sowing Impairs Grain Yield and Quality of Quinoa Genotypes Facing Drought and Salt Stress Under Field Conditions 晚播导致的热应激损害了田间条件下面临干旱和盐胁迫的藜麦基因型的谷物产量和质量
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-25 DOI: 10.1111/jac.12717
Ghulam Abbas, Behzad Murtaza, Muhammad Amjad, Muhammad Saqib, Muhammad Akram, Muhammad Asif Naeem, Ghulam Mustafa Shah, Mohsin Raza, Qasim Ali, Khalil Ahmed

Climate change is causing drastic reduction in crop yields around the globe due to increase in soil salinity, drought and heat stress. Quinoa (Chenopodium quinoa Willd) is regarded as a very significant food security crop considering the climate change scenario. Two quinoa genotypes (Puno and Titicaca) were cultivated on salt affected soil under drought stress with different sowing dates. Compared with early sowing, late sowing combined with salinity and drought stress caused drastic decline in plant growth and grain yield due to imposition of heat stress. Plant biomass and grain yield decreased by 26% and 39% in Puno, and by 34% and 49% in Titicaca under late sowing accompanied by salt and drought stress. Relative water contents and stomatal conductance of leaves declined in the same trend in both genotypes. Shoot Na+ concentration was the highest whereas K+ concentration was the lowest in both genotypes when drought and salt stress were combined under late sowing. Grain minerals (Ca, Mg, Fe, Zn, Cu, K, P, N and Mn) and dietary contents (protein, lipids, carbohydrates and fibre) were decreased more under the combination of salinity and drought for late sowing as compared to early sowing. When salinity and drought stress were combined under late sowing, the contents of H2O2 and TBARS were 1.9 and 2.2-fold higher in Puno and 2.4 and 2.6-fold higher in Titicaca, respectively. The oxidative stress was mitigated by enhanced activities of antioxidant enzymes (CAT, SOD and POD) more in Puno than Titicaca. Plant biomass and grain yield were higher in Puno with better grain quality than Titicaca. Hence, this genotype should be cultivated on salt affected soils facing drought and high temperatures.

由于土壤盐碱化、干旱和热胁迫的加剧,气候变化正在导致全球作物产量急剧下降。考虑到气候变化情况,藜麦(Chenopodium quinoa Willd)被认为是一种非常重要的粮食安全作物。两种藜麦基因型(Puno 和 Titicaca)以不同的播种日期在干旱胁迫下受盐分影响的土壤中种植。与早播相比,晚播加上盐分和干旱胁迫会导致热胁迫引起的植物生长和谷物产量急剧下降。在晚播并伴有盐胁迫和干旱胁迫的情况下,普诺的植物生物量和谷物产量分别减少了 26% 和 39%,的的喀喀湖的植物生物量和谷物产量分别减少了 34% 和 49%。两种基因型叶片的相对含水量和气孔导度呈相同的下降趋势。在干旱和盐胁迫同时存在的晚播条件下,两种基因型的芽中 Na+ 浓度最高,而 K+ 浓度最低。与早播相比,晚播在盐度和干旱胁迫下,谷物矿物质(钙、镁、铁、锌、铜、钾、磷、氮和锰)和膳食含量(蛋白质、脂类、碳水化合物和纤维)下降幅度更大。当盐度和干旱胁迫同时存在时,普诺的 H2O2 和 TBARS 含量分别是早播的 1.9 倍和 2.2 倍,的的喀喀湖的 2.4 倍和 2.6 倍。普诺的抗氧化酶(CAT、SOD 和 POD)活性高于的的喀喀省,从而减轻了氧化胁迫。与 Titicaca 相比,Puno 的植株生物量和谷物产量更高,谷物品质更好。因此,该基因型应在面临干旱和高温的盐渍土上种植。
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引用次数: 0
Optimising Sowing Window for Wheat Cultivars Under RCP 4.5 and RCP 6.0 Scenarios During the 21st Century in Indian Punjab 21 世纪印度旁遮普省在 RCP 4.5 和 RCP 6.0 情景下优化小麦品种播种期
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-21 DOI: 10.1111/jac.12711
Prabhjyot-Kaur, Sandeep Singh Sandhu, Shivani Kothiyal

A simulation study was conducted for assessing the climate change impact on wheat cultivars (HD2967 and PBW725) under RCP 4.5 and RCP 6.0 scenarios for four agroclimatic zones (AZ) of Punjab. The yield trend during 70 years (2025–95) using the CERES-Wheat model was assessed for different sowing windows (end October to end November). The maximum/minimum temperature and rainfall, respectively, during the season varied between 25–27°C/9–12°C and 27–103 mm (AZ II), 24–27°C/8–13°C and 37–105 mm (AZ III), 24–26°C/9–12°C and 20–80 mm (AZ IV) and 23–26°C/9–12°C and 30–71 mm (AZ V). The climatic conditions largely vary across the state, because of which only AZs II, III and V were found productive for wheat crop with most of the years lying in the high yield (>5000 kg/ha) category. The sowing of HD2967 during mid to end November would be suitable adaptation strategy for wheat growers in the state.

在 RCP 4.5 和 RCP 6.0 情景下,对旁遮普省的四个农业气候区(AZ)进行了模拟研究,以评估气候变化对小麦品种(HD2967 和 PBW725)的影响。利用 CERES 小麦模型评估了不同播种期(10 月底至 11 月底)70 年(2025-95 年)的产量趋势。该季节的最高/最低温度和降雨量分别为 25-27°C/9-12°C 和 27-103 毫米(AZ II)、24-27°C/8-13°C 和 37-105 毫米(AZ III)、24-26°C/9-12°C 和 20-80 毫米(AZ IV)以及 23-26°C/9-12°C 和 30-71 毫米(AZ V)。全州各地的气候条件差异很大,因此只有第二、第三和第五行政区的小麦作物产量较高,大部分年份都属于高产(>5000 公斤/公顷)类别。在 11 月中下旬播种 HD2967 将是该州小麦种植者的适当适应策略。
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引用次数: 0
Genome-Wide Identification of CLE Gene Family and Function Analysis of SbCLE39 Under Salt Stress in Sorghum 高粱盐胁迫下 CLE 基因家族的全基因组鉴定和 SbCLE39 的功能分析
IF 3.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-18 DOI: 10.1111/jac.12714
Zengting Chen, Yanling Zhang, Xin Xue, Haowei Tian, Ying Kong, Guocheng Ren

CLE proteins are a class of signalling factors involved in plant growth and abiotic stress response. They play crucial roles in processes such as cell differentiation, chlorophyll synthesis and abscisic acid (ABA) signal transduction. However, the function of the CLE genes in Sorghum bicolor remains unclear. In this study, 42 sorghum CLE genes were identified, and their evolutionary relationship, gene structure, amino acid sequence and homologous genes were analysed. We also examined the expression levels of CLE genes under various treatment conditions. Transcriptome data showed that there were significant differences in the expression patterns of 42 CLE genes in different tissues and organs. It is worth noting that SbCLE39 is mainly highly expressed in sorghum roots. At the same time, the expression of SbCLE39 decreased significantly under salt and ABA treatment. Compared with wild-type yeast cells (EV), yeast cells with high expression of SbCLE39 had lower tolerance to salt stress. In addition, the excessive accumulation of ABA caused by external application of SbCLE39p reduced the salt tolerance of sorghum. These findings suggest that SbCLE39 negatively regulates the salt tolerance of sorghum. These results lay a foundation for revealing the mechanism of CLE genes regulating the salt tolerance of sorghum and are of great significance for the cultivation of salt-tolerant crops.

CLE 蛋白是一类参与植物生长和非生物胁迫响应的信号因子。它们在细胞分化、叶绿素合成和脱落酸(ABA)信号转导等过程中发挥着至关重要的作用。然而,CLE 基因在双色高粱(Sorghum bicolor)中的功能仍不清楚。本研究鉴定了 42 个高粱 CLE 基因,分析了它们的进化关系、基因结构、氨基酸序列和同源基因。我们还研究了不同处理条件下 CLE 基因的表达水平。转录组数据显示,42 个 CLE 基因在不同组织器官中的表达模式存在显著差异。值得注意的是,SbCLE39 主要在高粱根部高表达。同时,在盐和 ABA 处理下,SbCLE39 的表达量明显下降。与野生型酵母细胞(EV)相比,高表达 SbCLE39 的酵母细胞对盐胁迫的耐受性较低。此外,外部施用 SbCLE39p 导致的 ABA 过度积累也降低了高粱的耐盐性。这些发现表明,SbCLE39 对高粱的耐盐性有负向调节作用。这些结果为揭示CLE基因调控高粱耐盐性的机制奠定了基础,对耐盐作物的培育具有重要意义。
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引用次数: 0
Short-Term Soil Waterlogging Improves Cotton Tolerance to High Temperature by Triggering Antioxidant Defence System in Cotton Seedlings 短期土壤涝害通过触发棉花幼苗的抗氧化防御系统提高棉花对高温的耐受性
IF 3.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-18 DOI: 10.1111/jac.12713
Haimiao Wang, Li Huang, Pan Yang, Xianghua Zeng, Yuli Huang, Wenting Yuan, Yixuan Kou, Zhiyong Zhang

Soil waterlogging and high temperature (HT) are serious abiotic stresses that negatively affect cotton growth and yield. Yet effects of prewaterlogging to HT subsequently in cotton seedlings have not been obtained. To address this, two temperature conditions (30/20°C and 35/25°C) and two soil waterlogging levels (0 and 3 days) were established during the cotton seedling stage. Results showed that indexes of plant performance were decreased markedly under HT. Unexpectedly, plant performance for the treatment of HT combined with 3 days of soil waterlogging (HW) was better than HT treatment (specifically, increase of 7.9%, 9.0%, 10.2%, 5.4% and 4.6% in leaf area, plant height, belowground biomass, aboveground biomass and root-to-shoot ratio was detected). Decreases in MDA (malondialdehyde), H2O2 (hydrogen peroxide) contents and O2$$ {mathrm{O}}_2^{-} $$ (superoxide radicals) generation rate under HW treatment were observed by 14.1%, 7.7% and 14.1%, respectively, compared with HT. Moreover, ASA (ascorbic acid) content and DHAR (dehydroascorbate reductase) activity were improved by 19.7% and 13.8% for HW treatment relative to HT, however, the opposite situation for activities of APX (ascorbate peroxidase) and GR (glutathione reductase). Besides, activities of SOD (superoxide dismutase), CAT (catalase) and POD (peroxidase) in HW treatment were increased by 16.7%, 8.3% and 18.4%, separately. Thus, we concluded that short-term soil waterlogging improved cotton cross-tolerance to the continued high-HT stress by enhanced SOD, CAT, POD and DHAR activities, increased ASA content in cotton seedlings. These results were expected to provide a theoretical basis for understanding cotton's cross-tolerance to abiotic stress.

土壤涝害和高温(HT)是严重的非生物胁迫,对棉花的生长和产量产生不利影响。然而,尚未获得棉花幼苗受涝前对高温随后的影响。为此,在棉花幼苗期设定了两种温度条件(30/20°C 和 35/25°C)和两种土壤积水程度(0 天和 3 天)。结果表明,在高温条件下,植株性能指标明显下降。出乎意料的是,高温热害结合 3 天土壤积水(HW)处理的植株表现优于高温热害处理(具体而言,叶面积、株高、地下生物量、地上生物量和根芽比分别增加了 7.9%、9.0%、10.2%、5.4% 和 4.6%)。MDA(丙二醛)、H2O2(过氧化氢)含量和 O 2 - $$ {mathrm{O}}_2^{-}$ (超氧自由基)含量均有所下降。$$ (超氧自由基)的产生率分别降低了 14.1%、7.7% 和 14.1%。此外,与 HT 相比,HW 处理的 ASA(抗坏血酸)含量和 DHAR(脱氢抗坏血酸还原酶)活性分别提高了 19.7% 和 13.8%,但 APX(抗坏血酸过氧化物酶)和 GR(谷胱甘肽还原酶)活性则相反。此外,SOD(超氧化物歧化酶)、CAT(过氧化氢酶)和 POD(过氧化物酶)的活性在 HW 处理中分别提高了 16.7%、8.3% 和 18.4%。因此,我们得出结论:短期土壤涝害通过提高棉苗的 SOD、CAT、POD 和 DHAR 活性以及增加棉苗的 ASA 含量,改善了棉花对持续高寒胁迫的交叉耐受性。这些结果有望为理解棉花对非生物胁迫的交叉耐受性提供理论依据。
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引用次数: 0
Evaluating Water Stress Adaptation in Cotton: Multivariate Analysis in F6–F7 Generations for Yield, Fibre Quality and Variety Selection 评估棉花的水胁迫适应性:F6-F7代产量、纤维质量和品种选择的多变量分析
IF 3.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-06-18 DOI: 10.1111/jac.12716
Hatice Kübra Gören, Öner Canavar, Uğur Tan

The impact of drought stress on productivity of cotton (Gossypium hirsutum L.) is a well-known challenge in agricultural production, and concurrently, the question of whether using the same or different selection criteria in well-watered and water-deficit conditions to select drought-tolerant cotton varieties remains unclear. This study aimed to comprehensively assess the single plant progeny lines within the F6 and F7 generations for determine response to DS and select the tolerant lines within the F7 generation. Single plant progeny rows were established, with the deficit water condition comprising 108 and 136 single plants for the F6 and F7 generations, respectively, and the WW condition consisting of 120 and 156 single plants for the F6 and F7 generations, respectively, with four blocks in Augmented experimental design. These progeny rows have length of 12 m, incorporate five control varieties (Karizma, Gloria, Carla, Candia and Claudia) to facilitate a comprehensive comparison. The study findings showed that fibre length, boll number and lint percentage were identified as the most crucial selection criteria under both WW and deficit irrigation conditions through principal component analysis. These indicators are highly beneficial for evaluating cotton's drought tolerance and screening potential drought-tolerant lines under both irrigation scenarios. According to the decision tree analysis, FL and BN have emerged as the most critical decision-making parameter in both irrigation conditions. Furthermore, the analysis revealed that each selection criterion has different impact in the comprehensive selection process. Also, as a result of all statistical analysis results and breeder observations, a total of 10 cotton lines were selected in the F7 generation. These selected genotypes hold promise for future cotton breeding programmes, providing an avenue to enhance drought tolerance and elevate cotton yield and productivity.

Clinical Trial Registration: This study does not involve a clinical trial, and therefore, clinical trial registration is not applicable.

干旱胁迫对棉花(Gossypium hirsutum L.)产量的影响是农业生产中一个众所周知的挑战,同时,在水分充足和缺水条件下使用相同或不同的选择标准来选择耐旱棉花品种的问题仍不清楚。本研究旨在全面评估 F6 代和 F7 代单株后代对干旱的反应,并在 F7 代中筛选出耐旱品系。研究建立了单株后代行,缺水条件下 F6 代和 F7 代分别有 108 株和 136 株单株后代,WW 条件下 F6 代和 F7 代分别有 120 株和 156 株单株后代。这些后代行长 12 米,包括五个对照品种(Karizma、Gloria、Carla、Candia 和 Claudia),以便进行综合比较。研究结果表明,通过主成分分析,纤维长度、棉铃数和皮棉率被确定为在WW和缺水灌溉条件下最关键的选择标准。在两种灌溉条件下,这些指标都非常有利于评估棉花的耐旱性和筛选潜在的耐旱品系。根据决策树分析,FL 和 BN 成为两种灌溉条件下最关键的决策参数。此外,分析表明,每个选择标准在综合选择过程中都有不同的影响。此外,根据所有统计分析结果和育种者的意见,F7 代共选出了 10 个棉花品系。这些被选中的基因型为未来的棉花育种计划提供了希望,为增强抗旱性、提高棉花产量和生产率提供了途径:本研究不涉及临床试验,因此不适用临床试验注册。
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引用次数: 0
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Journal of Agronomy and Crop Science
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