Pub Date : 2022-12-03DOI: 10.55218/jasr.2022131010
Ankita M. Kadam, Pradnya N. Jagtap, Sumit K. Musale, Vaishnavi P. More, Shweta S. Bobade, Pratibha P. Deshmukh, Komal D. Pol, Ashwini M. Kunjir
In vivo Absorption investigations are time-consuming and expensive in order to find the best medication formulation.Models were employed to research drug permeability and dissolution in order to streamline the search for arecommended formulation. One example of an in vitro model is the use of a goat intestinal section (sac) to assess theabsorption of related drugs prior to formulation and clinical investigations. The investigation of excipient and additiveeffects on drug permeability is an intriguing use of this method. In this paper, the impact of epigallocatechin -3-gallate(EGCG), on the intestinal penetration of acetyl salicylic acid was investigated. When Aspirin was kept with green plantextract, the concentration of absorbed Aspirin was 12, 32 ,45, 49 and 51μg/ml after 15, 30, 45, 60 and 75 minrespectively; whereas 14 ,16 ,17 ,18 and 23 μg/ml of Aspirin was absorbed when kept alone. The study clearly showedthat the green tea extract enhances the absorption of Aspirin from goat intestine and this absorption enhancement activityof the plant is due to polyphenolic content, presence of polyphenols like catechins and epicatechins.
{"title":"EVALUATION OF THE IMPACT OF GREEN TEA PLANT EXTRACT ON INTESTINAL ABSORPTION OF ASPIRIN USING EVERTED SAC TECHNIQUE","authors":"Ankita M. Kadam, Pradnya N. Jagtap, Sumit K. Musale, Vaishnavi P. More, Shweta S. Bobade, Pratibha P. Deshmukh, Komal D. Pol, Ashwini M. Kunjir","doi":"10.55218/jasr.2022131010","DOIUrl":"https://doi.org/10.55218/jasr.2022131010","url":null,"abstract":"In vivo Absorption investigations are time-consuming and expensive in order to find the best medication formulation.Models were employed to research drug permeability and dissolution in order to streamline the search for arecommended formulation. One example of an in vitro model is the use of a goat intestinal section (sac) to assess theabsorption of related drugs prior to formulation and clinical investigations. The investigation of excipient and additiveeffects on drug permeability is an intriguing use of this method. In this paper, the impact of epigallocatechin -3-gallate(EGCG), on the intestinal penetration of acetyl salicylic acid was investigated. When Aspirin was kept with green plantextract, the concentration of absorbed Aspirin was 12, 32 ,45, 49 and 51μg/ml after 15, 30, 45, 60 and 75 minrespectively; whereas 14 ,16 ,17 ,18 and 23 μg/ml of Aspirin was absorbed when kept alone. The study clearly showedthat the green tea extract enhances the absorption of Aspirin from goat intestine and this absorption enhancement activityof the plant is due to polyphenolic content, presence of polyphenols like catechins and epicatechins.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78395559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Urine cytology is used for screening of exfoliated bladder cells from voided urine but lacks sensitivity. This study aims to check the efficacy of 5-aminolevulinic acid (5- ALA) fluorescence cytology and establish a high sensitivity approach in detecting flat, in-situ and/or small lesions that are hardly visible under conventional cystoscopy. Intracellular PPIX allows red fluorescence detection. In this study, 5-ALA fluorescent cytology using urine was compared with conventional cytology in the diagnosis of bladder tumors. In this prospective study, we compared the sensitivity and specificity between conventional cytology, 5-ALA fluorescent cytology and FDA approved commercially available kits (NMP-22 and BTA). The percentage of Protoporphyrin IX facilitated by 5-ALA was amplified in cancer urothelial cells compared to normal urothelial cells. The sensitivity of conventional cytology and 5-ALA fluorescent cytology was 64% and 96% respectively, whereas the specificity was 92% and 98.67% respectively. In conclusion, 5-ALA induced fluorescent urine cytology demonstrated promising outcomes in the detection of bladder carcinoma cells. Furthermore, low grade and low stage tumor cells as well as flat lesions were also positively and accurately interpreted using 5-ALA fluorescent cytology.
{"title":"EMERGING ROLE OF URINARY BIOMARKERS IN DETECTION OF UROTHELIAL BLADDER CARCINOMA IN SOUTH INDIAN POPULATION","authors":"Rangrez Shadab, S. Bidi, S. Ghagane, R. Nerli","doi":"10.55218/jasr.202213914","DOIUrl":"https://doi.org/10.55218/jasr.202213914","url":null,"abstract":"Urine cytology is used for screening of exfoliated bladder cells from voided urine but lacks sensitivity. This study aims to check the efficacy of 5-aminolevulinic acid (5- ALA) fluorescence cytology and establish a high sensitivity approach in detecting flat, in-situ and/or small lesions that are hardly visible under conventional cystoscopy. Intracellular PPIX allows red fluorescence detection. In this study, 5-ALA fluorescent cytology using urine was compared with conventional cytology in the diagnosis of bladder tumors. In this prospective study, we compared the sensitivity and specificity between conventional cytology, 5-ALA fluorescent cytology and FDA approved commercially available kits (NMP-22 and BTA). The percentage of Protoporphyrin IX facilitated by 5-ALA was amplified in cancer urothelial cells compared to normal urothelial cells. The sensitivity of conventional cytology and 5-ALA fluorescent cytology was 64% and 96% respectively, whereas the specificity was 92% and 98.67% respectively. In conclusion, 5-ALA induced fluorescent urine cytology demonstrated promising outcomes in the detection of bladder carcinoma cells. Furthermore, low grade and low stage tumor cells as well as flat lesions were also positively and accurately interpreted using 5-ALA fluorescent cytology.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85349539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U. Desai, Sangita A. Kale, Haya Alkhaldi, Vinay Manocha
Aceclofenac is a commonly used NSAID drug for treatment of pain and inflammation. Aceclofenac has poor water solubility, hence it corresponds to BCS class II drug. Poor solubility leads to poor oral bioavailability, so a challenge for drug delivery. The objective of the present research work is to improve solubility of aceclofenac by using a novel combination of anti-solvent and sono-precipitation technique. Sonoprecipitation has several advantages over conventional methods, like it is cost effective, produces small particle, process is clean, etc. To make this formulation, we dissolved aceclofenac in ethanol and applied 250W sonification for 5 sec followed by 5 sec of interval. This process was repeated for 12 mins and produced micro-sized Aceclofenac dispersion. Further, we characterized and evaluated Aceclofenac dispersion by X-ray powder diffraction (XRPD), Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetric (DSC) and in vitro solubility and dissolution rate. The results shows that sonoprecipitation process led to the development of partially amorphous microparticles. The particle size range of 1-3 μm with mean diameter of 1.59 μm. The in-vitro studies indicated that the saturation solubility and dissolution rate of aceclofenac microcrystals were enhanced by 2.5 folds and 2-fold respectively, as compared to crude aceclofenac. In conclusion, the process of combining the antisolvent precipitation under sonication produced small, uniform, and stable aceclofenac microparticles with enhanced dissolution, solubility, and bioavaibility.
{"title":"ACECLOFENAC SOLUBILITY ENHANCEMENT BY SONO-PRECIPITATION METHOD: FORMULATION OPTIMIZATION, CHARACTERIZATION AND IN-VITRO EVALUATION","authors":"U. Desai, Sangita A. Kale, Haya Alkhaldi, Vinay Manocha","doi":"10.55218/jasr.202213906","DOIUrl":"https://doi.org/10.55218/jasr.202213906","url":null,"abstract":"Aceclofenac is a commonly used NSAID drug for treatment of pain and inflammation. Aceclofenac has poor water solubility, hence it corresponds to BCS class II drug. Poor solubility leads to poor oral bioavailability, so a challenge for drug delivery. The objective of the present research work is to improve solubility of aceclofenac by using a novel combination of anti-solvent and sono-precipitation technique. Sonoprecipitation has several advantages over conventional methods, like it is cost effective, produces small particle, process is clean, etc. To make this formulation, we dissolved aceclofenac in ethanol and applied 250W sonification for 5 sec followed by 5 sec of interval. This process was repeated for 12 mins and produced micro-sized Aceclofenac dispersion. Further, we characterized and evaluated Aceclofenac dispersion by X-ray powder diffraction (XRPD), Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetric (DSC) and in vitro solubility and dissolution rate. The results shows that sonoprecipitation process led to the development of partially amorphous microparticles. The particle size range of 1-3 μm with mean diameter of 1.59 μm. The in-vitro studies indicated that the saturation solubility and dissolution rate of aceclofenac microcrystals were enhanced by 2.5 folds and 2-fold respectively, as compared to crude aceclofenac. In conclusion, the process of combining the antisolvent precipitation under sonication produced small, uniform, and stable aceclofenac microparticles with enhanced dissolution, solubility, and bioavaibility.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84142691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L-proline is an organic material, potassium iodide is an inorganic material and these two materials were mixed to form a semi-organic material viz. L-proline potassium iodide (LPPI) crystal in this work. Single crystals of L-proline potassium iodide were grown in an aqueous solution with slow evaporation process. LPPI crystals were obtained after the growth period of 35 days. XRD studies revealed the grown LPPI crystal to be crystallizing in a tetragonal structure. The grown LPPI crystal was analyzed for mechanical stability. Dielectric studies of the LPPI crystals were carried out to evaluate the dielectric constant, dielectric loss and AC conductivity. UV-visible-NIR spectral studies were performed in the wavelength range 200-1100 nm, and linear optical properties like transmittance, absorption coefficient and extinction coefficient of the sample were evaluated. Using Kurtz-Perry technique, second order NLO studies of the grown crystal of LPPI were carried out. In order to confirm the functional groups of the prepared sample, FTIR study was taken. EDS study was done to find the weight percent of the elements in the sample. Electronic polarizability and LDT value of LPPI crystal were estimated and the results were analyzed.
l -脯氨酸是一种有机材料，碘化钾是一种无机材料，本文将这两种材料混合形成半有机材料即l -脯氨酸碘化钾晶体(LPPI)。采用缓慢蒸发法制备了l -脯氨酸碘化钾单晶。生长35 d后获得LPPI晶体。XRD研究表明，生长的LPPI晶体呈四方晶型。对生长后的LPPI晶体进行了力学稳定性分析。研究了LPPI晶体的介电常数、介电损耗和交流电导率。在200-1100 nm波长范围内进行紫外-可见-近红外光谱研究，评估样品的透过率、吸收系数和消光系数等线性光学性质。利用Kurtz-Perry技术，对LPPI生长晶体进行了二阶NLO研究。为了确定所制备样品的官能团，进行了FTIR研究。EDS研究是为了找出样品中元素的重量百分比。对LPPI晶体的电子极化率和LDT值进行了估计，并对结果进行了分析。
{"title":"SYNTHESIS AND CHARACTERIZATION OF L-PROLINE POTASSIUM IODIDE CRYSTAL","authors":"L. R. Latha, P. Selvarajan, J. Maheswari","doi":"10.55218/jasr.202213913","DOIUrl":"https://doi.org/10.55218/jasr.202213913","url":null,"abstract":"L-proline is an organic material, potassium iodide is an inorganic material and these two materials were mixed to form a semi-organic material viz. L-proline potassium iodide (LPPI) crystal in this work. Single crystals of L-proline potassium iodide were grown in an aqueous solution with slow evaporation process. LPPI crystals were obtained after the growth period of 35 days. XRD studies revealed the grown LPPI crystal to be crystallizing in a tetragonal structure. The grown LPPI crystal was analyzed for mechanical stability. Dielectric studies of the LPPI crystals were carried out to evaluate the dielectric constant, dielectric loss and AC conductivity. UV-visible-NIR spectral studies were performed in the wavelength range 200-1100 nm, and linear optical properties like transmittance, absorption coefficient and extinction coefficient of the sample were evaluated. Using Kurtz-Perry technique, second order NLO studies of the grown crystal of LPPI were carried out. In order to confirm the functional groups of the prepared sample, FTIR study was taken. EDS study was done to find the weight percent of the elements in the sample. Electronic polarizability and LDT value of LPPI crystal were estimated and the results were analyzed.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86929186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neomycin sulphate is official in IP 2018, USP 2004 and BP 2003; which includes Microbial assay (Cylinder Plate assay) for the estimation therefore chemical derivatization of Neomycin Sulphate is done using 1-Fluoro-2, 4-Dinitrobenzene (DNFB); also known as Sanger’s reagent, to make it detectable by UV with good accuracy and precision. A fixed dose combination of Neomycin Sulphate, Clobetasol Propionate and Chlorocresol (preservative) in cream formulation is used to treat different types of Skin infections. The literature review reveals that methods like UV, HPLC, HPTLC, GC-MS etc. have been reported for Neomycin Sulphate and Clobetasol Propionate individually and along with other drugs. But no reported method found in combination for Neomycin sulphate and Clobetasol propionate. A simple, accurate, precise and economical UV spectrophotometric i.e. Area Under Curve Method (AUC) was developed. All the dilutions of drugs were prepared in Methanol: Acetonitrile (50:50). Area under curve was integrated in the wavelength range of 235.0- 245.0 nm, 252.0-262.0 nm and 223.0-233.0 nm for CSP, NMS and CCS respectively. The AUC method for Clobetasol Propionate (CSP), Neomycin Sulphate (NMS) and Chlorocresol (CCS) was found to be linear over the range of 0.8-1.2 μg/ml, 8.0-12.0 μg/ml and 1.6-2.4 μg/ml respectively. The result of analysis was analysed and validated statistically and recovery study was found within range of 98-102%. The % RSD was not more than 2.0 % which indicates good precision. All the validation parameters were carried out according to ICH Q2 (R1) guidelines.
{"title":"DERIVATIZATION OF NEOMYCIN SULPHATE AND AREA UNDER CURVE METHOD FOR ESTIMATION OF NEOMYCIN SULPHATE AND CLOBETASOL PROPIONATE IN CREAM","authors":"Alisha P. Patel, Heli Desai, Ankita Patel","doi":"10.55218/jasr.202213912","DOIUrl":"https://doi.org/10.55218/jasr.202213912","url":null,"abstract":"Neomycin sulphate is official in IP 2018, USP 2004 and BP 2003; which includes Microbial assay (Cylinder Plate assay) for the estimation therefore chemical derivatization of Neomycin Sulphate is done using 1-Fluoro-2, 4-Dinitrobenzene (DNFB); also known as Sanger’s reagent, to make it detectable by UV with good accuracy and precision. A fixed dose combination of Neomycin Sulphate, Clobetasol Propionate and Chlorocresol (preservative) in cream formulation is used to treat different types of Skin infections. The literature review reveals that methods like UV, HPLC, HPTLC, GC-MS etc. have been reported for Neomycin Sulphate and Clobetasol Propionate individually and along with other drugs. But no reported method found in combination for Neomycin sulphate and Clobetasol propionate. A simple, accurate, precise and economical UV spectrophotometric i.e. Area Under Curve Method (AUC) was developed. All the dilutions of drugs were prepared in Methanol: Acetonitrile (50:50). Area under curve was integrated in the wavelength range of 235.0- 245.0 nm, 252.0-262.0 nm and 223.0-233.0 nm for CSP, NMS and CCS respectively. The AUC method for Clobetasol Propionate (CSP), Neomycin Sulphate (NMS) and Chlorocresol (CCS) was found to be linear over the range of 0.8-1.2 μg/ml, 8.0-12.0 μg/ml and 1.6-2.4 μg/ml respectively. The result of analysis was analysed and validated statistically and recovery study was found within range of 98-102%. The % RSD was not more than 2.0 % which indicates good precision. All the validation parameters were carried out according to ICH Q2 (R1) guidelines.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90674242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Effects of 1, 8, 15, 22-tetranitro Co(II) and Zn(II) phthalocyanines [3-NO2CoPc, 3-NO2ZnPc], 1,8,15,22-tetracyano Co(II) phthalocyanine [3-CNCoPc] and 2, 9, 16, 23-tetranitro iron(III)chlorophthalocyanine [4-NO2FePc] on the growth of the fungus Aspergillus niger were studied. All the four complexes inhibited the radial spread of the fungus selected for the study to different extents. A considerable inhibitory effect on growth was seen even in l ppm concentration. The order of inhibitory effect, measured for a period of 5 days is 3-NO2 ZnPc > 3-CNCoPc > 3-NO2CoPc > 4-NO2FePc. The presence of the peripherally substituted phthalocyanine complexes is found to alter the pigmentation property of the selected fungus. Safranin-phthalocyanine double staining technique using 3-NO2CoPc, 3-NO2ZnPc, 4-NO2FePc and 2, 9, 16, 23-tetrahydroxy iron(III)chlorophthalocyanine [4-OHFePc] on the stem sections of Hemidesmus indicus R Br and Polygonum punctatum Buch-Hum have been studied.
{"title":"ANTIFUNGAL ACTIVITY AND STAINING PROPERTIES OF SOME SYMMETRICALLY FUNCTIONALIZED METAL PHTHALOCYANINE DYES","authors":"M. P. Somashekarappa, J. Keshavayya","doi":"10.55218/jasr.202213911","DOIUrl":"https://doi.org/10.55218/jasr.202213911","url":null,"abstract":"Effects of 1, 8, 15, 22-tetranitro Co(II) and Zn(II) phthalocyanines [3-NO2CoPc, 3-NO2ZnPc], 1,8,15,22-tetracyano Co(II) phthalocyanine [3-CNCoPc] and 2, 9, 16, 23-tetranitro iron(III)chlorophthalocyanine [4-NO2FePc] on the growth of the fungus Aspergillus niger were studied. All the four complexes inhibited the radial spread of the fungus selected for the study to different extents. A considerable inhibitory effect on growth was seen even in l ppm concentration. The order of inhibitory effect, measured for a period of 5 days is 3-NO2 ZnPc > 3-CNCoPc > 3-NO2CoPc > 4-NO2FePc. The presence of the peripherally substituted phthalocyanine complexes is found to alter the pigmentation property of the selected fungus. Safranin-phthalocyanine double staining technique using 3-NO2CoPc, 3-NO2ZnPc, 4-NO2FePc and 2, 9, 16, 23-tetrahydroxy iron(III)chlorophthalocyanine [4-OHFePc] on the stem sections of Hemidesmus indicus R Br and Polygonum punctatum Buch-Hum have been studied.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88689228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Essential oil-bearing medicinal and aromatic plant Pelargonium graveolense is commonly known as rose-scented geranium because of its characteristic rose like smell. Its essential oil possesses medicinal properties such as insect repellent properties and also used in fragrance and food industry. Its demand is very high because of the above use in India and the worldwide market. This experiment is designed to develop an agro technique method for large scale production of geranium plantlets (cultivar CIM-Pawan) at field conditions using simple stem cutting method at North Indian field conditions in Uttar Pradesh, India. And also introduce a new method of intercropping cultivation of geranium in Brinjal (Solanum melongena) that is developed. This experiment was designed in such a manner to developed thousand plantlets from one year old a single mother plants. As we know near about 30,000 stem cuttings are required for planting an area of one hectare land. So focusing on large demands of plantlets developed an agro technique that is able to fulfil the requirements. The crops were raised using one month olds plantlets transplanting, viz. with intercropping in Brinjal. After harvesting oil was extracted by hydro-distillation using Clevenger apparatus and analyzed by gas chromatography (GC). The Citronellol/Geraniol ratio (C: G), quality determining factor was found to 1.23. The GC and GC-MS analysis results indicated that the essential oil components, quality and quantity were significant. The major constituents of the essential oil were Citronellol (29.81%), Geraniol (24.23%), Linalool (3.12%), Iso-menthone (4.09%), Citronellyl formate (7.51%), Geranyl formate (6.07%), and 10-epi-γ-eudesmol (2.70%).
{"title":"DEVELOPMENT OF AN AGRO TECHNIQUE FOR LARGE-SCALE PRODUCTION OF GERANIUM’S PLANTLETS FOR CULTIVATION AND BRINJAL INTERCROPPING","authors":"S. K. Bose, R. Bansal, N. Sangwan","doi":"10.55218/jasr.202213905","DOIUrl":"https://doi.org/10.55218/jasr.202213905","url":null,"abstract":"Essential oil-bearing medicinal and aromatic plant Pelargonium graveolense is commonly known as rose-scented geranium because of its characteristic rose like smell. Its essential oil possesses medicinal properties such as insect repellent properties and also used in fragrance and food industry. Its demand is very high because of the above use in India and the worldwide market. This experiment is designed to develop an agro technique method for large scale production of geranium plantlets (cultivar CIM-Pawan) at field conditions using simple stem cutting method at North Indian field conditions in Uttar Pradesh, India. And also introduce a new method of intercropping cultivation of geranium in Brinjal (Solanum melongena) that is developed. This experiment was designed in such a manner to developed thousand plantlets from one year old a single mother plants. As we know near about 30,000 stem cuttings are required for planting an area of one hectare land. So focusing on large demands of plantlets developed an agro technique that is able to fulfil the requirements. The crops were raised using one month olds plantlets transplanting, viz. with intercropping in Brinjal. After harvesting oil was extracted by hydro-distillation using Clevenger apparatus and analyzed by gas chromatography (GC). The Citronellol/Geraniol ratio (C: G), quality determining factor was found to 1.23. The GC and GC-MS analysis results indicated that the essential oil components, quality and quantity were significant. The major constituents of the essential oil were Citronellol (29.81%), Geraniol (24.23%), Linalool (3.12%), Iso-menthone (4.09%), Citronellyl formate (7.51%), Geranyl formate (6.07%), and 10-epi-γ-eudesmol (2.70%).","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83584694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lagerstroemia speciosa medicinal properties suggest that it may have anti-inflammatory effects on acute inflammation. The present study was performed to investigate the anti-inflammatory effect of L. speciosa ethanolic leaf, flower, and seed extract (LELE, LEFE, and LESE) on carrageenan-induced acute inflammation in rats. This plant is commonly called Pride of India refers to the various parts of this plant, including the leaves, flowers, and seeds, which are used as folk medicine. It has been demonstrated that the phytochemical and anti-inflammatory activities are effective in the treatment of inflammatory conditions in traditional medicine. The qualitative phytochemical investigation of L. speciosa leaf, flower, and seed revealed that it contained steroids, terpenoids, glycosides, polyphenolic compounds, amino acids, saponins, alkaloids, flavonoids, reducing sugars, tannins, and many other active metabolites. Studies have shown active antiinflammatory ingredients in L. speciosa ethanolic extracts of leaf (LELE), flower (LEFE), and seed (LESE). The ethanolic extracts of L. speciosa (LELE, LEFE and LESE) were investigated for anti-inflammatory activity in carrageenan-induced paw edema in albino rats and compared to control and standard drug, Diclofenac injection. The experimental plant extracts were given orally at a concentration of 250 and 500 mg/kg b.w. before carrageenan injection. In the paw edema evaluation, the paw volume was measured in the early (from 0 min-3 h) and late (4-6 h) phases of edema formation. In acute inflammation paw models, the ethanol extracts significantly reduced paw edema. The result obtained is indicative that the ethanolic extract of leaf and seed with high dose of 500 mg/kg b.w. produced 42% of inhibition and is low as compared to the standard drug. Low dose of ethanolic flower extract of L. speciosa inhibitory is equal to the low dose of seed extract; it is 1% lower than the standard and 2% lower than the control.
{"title":"EVALUATION OF ANTI-INFLAMMATORY PROPERTIES OF ETHANOLIC LEAF, FLOWER AND SEED EXTRACTS OF LAGERSTROEMIA SPECIOSA (L.) PERS (LYTHRACEAE) AGAINST CARRAGEENAN-INDUCED ACUTE INFLAMMATION IN ALBINO RATS","authors":"S. Pavithran, P. Sujatha","doi":"10.55218/jasr.202213907","DOIUrl":"https://doi.org/10.55218/jasr.202213907","url":null,"abstract":"Lagerstroemia speciosa medicinal properties suggest that it may have anti-inflammatory effects on acute inflammation. The present study was performed to investigate the anti-inflammatory effect of L. speciosa ethanolic leaf, flower, and seed extract (LELE, LEFE, and LESE) on carrageenan-induced acute inflammation in rats. This plant is commonly called Pride of India refers to the various parts of this plant, including the leaves, flowers, and seeds, which are used as folk medicine. It has been demonstrated that the phytochemical and anti-inflammatory activities are effective in the treatment of inflammatory conditions in traditional medicine. The qualitative phytochemical investigation of L. speciosa leaf, flower, and seed revealed that it contained steroids, terpenoids, glycosides, polyphenolic compounds, amino acids, saponins, alkaloids, flavonoids, reducing sugars, tannins, and many other active metabolites. Studies have shown active antiinflammatory ingredients in L. speciosa ethanolic extracts of leaf (LELE), flower (LEFE), and seed (LESE). The ethanolic extracts of L. speciosa (LELE, LEFE and LESE) were investigated for anti-inflammatory activity in carrageenan-induced paw edema in albino rats and compared to control and standard drug, Diclofenac injection. The experimental plant extracts were given orally at a concentration of 250 and 500 mg/kg b.w. before carrageenan injection. In the paw edema evaluation, the paw volume was measured in the early (from 0 min-3 h) and late (4-6 h) phases of edema formation. In acute inflammation paw models, the ethanol extracts significantly reduced paw edema. The result obtained is indicative that the ethanolic extract of leaf and seed with high dose of 500 mg/kg b.w. produced 42% of inhibition and is low as compared to the standard drug. Low dose of ethanolic flower extract of L. speciosa inhibitory is equal to the low dose of seed extract; it is 1% lower than the standard and 2% lower than the control.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76083942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Among all the nanoparticles synthesized; silver nanoparticles have attained special place in the area of nano technology because of their antimicrobial, nontoxic, environmentally safe and biomedical applications. In general; their syntheses involves the use of hazardous chemicals or costly physical methods. However, the biological processes are making their ways in between and proving their advantages over them. The use of plants and their extracts is one of the most valuable methods which are gaining concerns due to their imperative biological benefits. Plants are not only beautiful but majestic because they are rich sources of various medicinally important substances. They explore the huge diversity which can be utilized towards rapid and single step protocol preparatory method for various nanoparticles keeping intact “the green principles” over the conventional ones and proving their dominance for medicinal importance. Here, in the presented work “one pot synthesis of silver nanoparticles” is described. Therefore; a simple, cost effective bio-reduction on the principle of “green synthesis” of silver nanoparticles using the Alchemilla vulgaris plant extract is reported. The beauty of the synthesis is: no involvement of any surfactant, catalyst or template. The aqueous silver ions are reduced to silver nanoparticles when exposed to plant extract. The bio-reduction and stabilization of so formed silver nanoparticles was monitored by UV-Vis spectrophotometry, FTIR spectroscopy, SEM, particle size and zeta potential.
{"title":"GREEN SYNTHESIS AND CHARACTERIZATION OF ALCHEMILLA VULGARIS SILVER NANOPARTICLES","authors":"Shilpee Jain, A. Yadav, R. Gothalwal","doi":"10.55218/jasr.202213909","DOIUrl":"https://doi.org/10.55218/jasr.202213909","url":null,"abstract":"Among all the nanoparticles synthesized; silver nanoparticles have attained special place in the area of nano technology because of their antimicrobial, nontoxic, environmentally safe and biomedical applications. In general; their syntheses involves the use of hazardous chemicals or costly physical methods. However, the biological processes are making their ways in between and proving their advantages over them. The use of plants and their extracts is one of the most valuable methods which are gaining concerns due to their imperative biological benefits. Plants are not only beautiful but majestic because they are rich sources of various medicinally important substances. They explore the huge diversity which can be utilized towards rapid and single step protocol preparatory method for various nanoparticles keeping intact “the green principles” over the conventional ones and proving their dominance for medicinal importance. Here, in the presented work “one pot synthesis of silver nanoparticles” is described. Therefore; a simple, cost effective bio-reduction on the principle of “green synthesis” of silver nanoparticles using the Alchemilla vulgaris plant extract is reported. The beauty of the synthesis is: no involvement of any surfactant, catalyst or template. The aqueous silver ions are reduced to silver nanoparticles when exposed to plant extract. The bio-reduction and stabilization of so formed silver nanoparticles was monitored by UV-Vis spectrophotometry, FTIR spectroscopy, SEM, particle size and zeta potential.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79701270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Duraivathi, J. Jeya, J. Poongodi, H. J. Johnson
An endeavor has been made to report the herbal synthesis of chromium oxide (Cr2O3) nanoparticles using aqueous extract of nigella sativa seed extract as a capping agent and chromic sulphateas a precursor material. The structural, morphological and optical properties of the Cr2O3 nanoparticles were analyzed by X - ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and UV- Visible spectroscopy. The antibacterial efficacy of chromium oxide nanoparticles was tested against Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa. The photocatalytic activity of biosynthesized Cr2O3 nanoparticles was evaluated by degrading methylene blue dye and Congo red dye under the irradiation of visible light. Apart from this, ultrasonic velocity, density and viscosity were measured for different concentrations of Cr2O3 nanofluid using ultrasonic techniques. Thermoacoustical such as adiabatic compressibility, free length, acoustic impedance, relaxation time and absorption coefficient values are computed from the measured data and the results are interpreted in terms of particle - fluid interactions.
{"title":"GREEN SYNTHESIS OF CHROMIUM OXIDE NANOPARTICLES - STUDY OF ITS ANTIBACTERIAL, PHOTOCATALYTIC AND THERMODYNAMIC PROPERTIES","authors":"C. Duraivathi, J. Jeya, J. Poongodi, H. J. Johnson","doi":"10.55218/jasr.202213904","DOIUrl":"https://doi.org/10.55218/jasr.202213904","url":null,"abstract":"An endeavor has been made to report the herbal synthesis of chromium oxide (Cr2O3) nanoparticles using aqueous extract of nigella sativa seed extract as a capping agent and chromic sulphateas a precursor material. The structural, morphological and optical properties of the Cr2O3 nanoparticles were analyzed by X - ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and UV- Visible spectroscopy. The antibacterial efficacy of chromium oxide nanoparticles was tested against Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa. The photocatalytic activity of biosynthesized Cr2O3 nanoparticles was evaluated by degrading methylene blue dye and Congo red dye under the irradiation of visible light. Apart from this, ultrasonic velocity, density and viscosity were measured for different concentrations of Cr2O3 nanofluid using ultrasonic techniques. Thermoacoustical such as adiabatic compressibility, free length, acoustic impedance, relaxation time and absorption coefficient values are computed from the measured data and the results are interpreted in terms of particle - fluid interactions.","PeriodicalId":14906,"journal":{"name":"Journal of Advanced Scientific Research","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86108959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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