Bronchial Asthma is a chronic inflammatory condition in lung airways resulting in episodic airflow obstruction [1]. Swasa Kasam mentioned in siddha literatures which is equated to Bronchial Asthma with the symptoms of breathlessness, cough with or without expectoration, tightness of chest, wheezing, elevated eosinophil count. Eosinophilia is generally observed in patients with a range of 500 to 2000 eosinophilia/µl in blood. Sai prasath et al [2]., uses ayurveda medicines in the treatment of eosinophilia in bronchial asthma and the author observed the anti-eosinophilic effect of the ayurvedic medicines. In this paper the author uses Siddha medicines (a traditional medicine system) to cure eosinophilia in Bronchial Asthma.This case study may be useful for clinicians practicing siddha system of medicine.
{"title":"A Case Study of Eosinophilia in Bronchial Asthma Through Siddha Medicine","authors":"R.Rushmi Kruthiga","doi":"10.31254/jahm.2023.9302","DOIUrl":"https://doi.org/10.31254/jahm.2023.9302","url":null,"abstract":"Bronchial Asthma is a chronic inflammatory condition in lung airways resulting in episodic airflow obstruction [1]. Swasa Kasam mentioned in siddha literatures which is equated to Bronchial Asthma with the symptoms of breathlessness, cough with or without expectoration, tightness of chest, wheezing, elevated eosinophil count. Eosinophilia is generally observed in patients with a range of 500 to 2000 eosinophilia/µl in blood. Sai prasath et al [2]., uses ayurveda medicines in the treatment of eosinophilia in bronchial asthma and the author observed the anti-eosinophilic effect of the ayurvedic medicines. In this paper the author uses Siddha medicines (a traditional medicine system) to cure eosinophilia in Bronchial Asthma.This case study may be useful for clinicians practicing siddha system of medicine.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"38 8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tribal and local forest dwellers possesses traditional knowledge of their surroundings plants with wide application for the treatment of various ailments. Plant-based herbal medicines have received great attention after post covid-19 scenario due to its economic, efficient, and minimal side effects. Diabetes mellitus is a well-known metabolic disorder of endocrine insulin hormone which makes it as a chronic disease. The present review focuses on the traditional knowledge of the medicinal plants used by the tribal and local people for the treatment and management of Diabetes in Telangana. The study is a combination of literature based data as well as field interaction with people which revealed that 100 species belonging to 45 families are being used directly or in combination with other plants for the treatment of Diabetes. Fabaceae was the dominant family followed by Apocynaceae and Cucurbitaceae while tree is the major habit followed by herbs and climbers. Leaves are mostly used in the drug preparations followed by bark and root/rhizome. It has been found that formulations of only 40 species are known while other 60 species are not disclosed. Among the known formulations, powder is mostly used for treatment followed by paste and decoctions. Overall the present comprehensive reviews have shown the potential of traditional medicinal plants and its related traditional knowledge for curing diabetes which provides a path for future bioprospecting. In addition to this, these species should be conserved and cultivated under in-situ and ex-situ programmes which are necessary for sustainable supply for raw material to the benefit of the society as well as to improve livelihood of tribal/local people.
{"title":"Traditional knowledge of Plants Used for the Treatment of Diabetes in Telangana, India: A Comprehensive Review","authors":"Pankaj Singh, J. M. Chandana, K. Chandraprakash","doi":"10.31254/jahm.2023.9308","DOIUrl":"https://doi.org/10.31254/jahm.2023.9308","url":null,"abstract":"Tribal and local forest dwellers possesses traditional knowledge of their surroundings plants with wide application for the treatment of various ailments. Plant-based herbal medicines have received great attention after post covid-19 scenario due to its economic, efficient, and minimal side effects. Diabetes mellitus is a well-known metabolic disorder of endocrine insulin hormone which makes it as a chronic disease. The present review focuses on the traditional knowledge of the medicinal plants used by the tribal and local people for the treatment and management of Diabetes in Telangana. The study is a combination of literature based data as well as field interaction with people which revealed that 100 species belonging to 45 families are being used directly or in combination with other plants for the treatment of Diabetes. Fabaceae was the dominant family followed by Apocynaceae and Cucurbitaceae while tree is the major habit followed by herbs and climbers. Leaves are mostly used in the drug preparations followed by bark and root/rhizome. It has been found that formulations of only 40 species are known while other 60 species are not disclosed. Among the known formulations, powder is mostly used for treatment followed by paste and decoctions. Overall the present comprehensive reviews have shown the potential of traditional medicinal plants and its related traditional knowledge for curing diabetes which provides a path for future bioprospecting. In addition to this, these species should be conserved and cultivated under in-situ and ex-situ programmes which are necessary for sustainable supply for raw material to the benefit of the society as well as to improve livelihood of tribal/local people.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"64 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manish Kumar Sharma, Pooja Pareek, Priyanka Sharma, Rajesh Sharma, D. C. Sharma
Ayurveda, the ancient system of traditional medicine, has been an integral part of various cultures and communities for centuries. With its holistic approach to health and well-being, Ayurveda offers a wealth of ancient wisdom that can potentially address contemporary health challenges. However, to validate and integrate Ayurvedic practices into modern healthcare systems, rigorous scientific research becomes indispensable. Standardization of Ayurvedic treatments is another vital aspect that can be achieved through systematic research. The establishment of standardized procedures ensures consistency in practice across different regions and among practitioners, enhancing the credibility of Ayurveda as a viable alternative or complementary system of medicine. Preserving and documenting Ayurvedic knowledge not only protects the cultural heritage but also enables the transmission of accurate information to future generations of practitioners and researchers. Research in Ayurveda is of utmost importance to unlock the profound potential of this traditional medical system through the lens of modern science. The integration of ancient wisdom and contemporary knowledge will pave the way for a more holistic and inclusive approach to healthcare, benefitting individuals and societies worldwide. This article emphasizes the critical need for research in Ayurveda to bridge the gap between traditional knowledge and modern scientific understanding. By employing modern scientific methods, researchers can validate the efficacy and safety of Ayurvedic treatments and practices, leading to wider acceptance and recognition within the medical community.
{"title":"The Imperative Need for Research in Ayurveda: Unraveling Ancient Wisdom through Modern Science","authors":"Manish Kumar Sharma, Pooja Pareek, Priyanka Sharma, Rajesh Sharma, D. C. Sharma","doi":"10.31254/jahm.2023.9301","DOIUrl":"https://doi.org/10.31254/jahm.2023.9301","url":null,"abstract":"Ayurveda, the ancient system of traditional medicine, has been an integral part of various cultures and communities for centuries. With its holistic approach to health and well-being, Ayurveda offers a wealth of ancient wisdom that can potentially address contemporary health challenges. However, to validate and integrate Ayurvedic practices into modern healthcare systems, rigorous scientific research becomes indispensable. Standardization of Ayurvedic treatments is another vital aspect that can be achieved through systematic research. The establishment of standardized procedures ensures consistency in practice across different regions and among practitioners, enhancing the credibility of Ayurveda as a viable alternative or complementary system of medicine. Preserving and documenting Ayurvedic knowledge not only protects the cultural heritage but also enables the transmission of accurate information to future generations of practitioners and researchers. Research in Ayurveda is of utmost importance to unlock the profound potential of this traditional medical system through the lens of modern science. The integration of ancient wisdom and contemporary knowledge will pave the way for a more holistic and inclusive approach to healthcare, benefitting individuals and societies worldwide. This article emphasizes the critical need for research in Ayurveda to bridge the gap between traditional knowledge and modern scientific understanding. By employing modern scientific methods, researchers can validate the efficacy and safety of Ayurvedic treatments and practices, leading to wider acceptance and recognition within the medical community.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"151 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr. Prathap Kumar, MN BalakrishnaNair, Punniyamurthy, Balakrishna Nair
Knowledge of the source/origin of a toxin, its development and toxicity manifestation as well as appropriate remedial measures is an integral part of any health system. Mycotoxins are chemical toxins produced by fungi with diverse toxic effects depending on their chemical structure. Modern science recognizes mycotoxins as food contaminants and recommends regulatory limits (max permissible limit), due to the impossibility in avoiding the mycotoxin occurrence completely and for the complications in treating the resulting toxicities. This brings traditional systems of medicine such as ayurveda into the limelight to address the global mycotoxin problem. In this regard, there is paucity of literature available describing the mycotoxins from ayurvedic perspectives. Thus, the current article is focused on the transdiciplinary understand of the mycotoxins and its toxic effects, especially comparing modern medicine with ayurveda. While, modern medicine considers mycotoxins as chemical toxins with molecular mechanism involving receptor or chemical binding, ayurveda considers a holistic approach. In addition to considering its effects on cellular level, ayurveda aligns to recognize the toxin for its effects on the system at tissue and organism level, holistically. From this prespective, ayurveda classifies mycotoxins as both incompatible food (virudha ahara) and visha (toxin). To understand the pathogenesis, ayurveda analyses the systemic effects in terms of effect on “the basic body composition - doshas (humors) and dhaatus (tissues) and the impact on toxin/metabolic waste elimination”. Finally, after considering the overall effect ayurveda arrives at the treatment to provide both symptomatic treatment and reinstate the doshas/dhaatus to restore the health status, holistically.
{"title":"Understanding Mycotoxins from Ayurvedic Perspectives: A Trans-disciplinary approach","authors":"Dr. Prathap Kumar, MN BalakrishnaNair, Punniyamurthy, Balakrishna Nair","doi":"10.31254/jahm.2023.9306","DOIUrl":"https://doi.org/10.31254/jahm.2023.9306","url":null,"abstract":"Knowledge of the source/origin of a toxin, its development and toxicity manifestation as well as appropriate remedial measures is an integral part of any health system. Mycotoxins are chemical toxins produced by fungi with diverse toxic effects depending on their chemical structure. Modern science recognizes mycotoxins as food contaminants and recommends regulatory limits (max permissible limit), due to the impossibility in avoiding the mycotoxin occurrence completely and for the complications in treating the resulting toxicities. This brings traditional systems of medicine such as ayurveda into the limelight to address the global mycotoxin problem. In this regard, there is paucity of literature available describing the mycotoxins from ayurvedic perspectives. Thus, the current article is focused on the transdiciplinary understand of the mycotoxins and its toxic effects, especially comparing modern medicine with ayurveda. While, modern medicine considers mycotoxins as chemical toxins with molecular mechanism involving receptor or chemical binding, ayurveda considers a holistic approach. In addition to considering its effects on cellular level, ayurveda aligns to recognize the toxin for its effects on the system at tissue and organism level, holistically. From this prespective, ayurveda classifies mycotoxins as both incompatible food (virudha ahara) and visha (toxin). To understand the pathogenesis, ayurveda analyses the systemic effects in terms of effect on “the basic body composition - doshas (humors) and dhaatus (tissues) and the impact on toxin/metabolic waste elimination”. Finally, after considering the overall effect ayurveda arrives at the treatment to provide both symptomatic treatment and reinstate the doshas/dhaatus to restore the health status, holistically.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jasmeet Kaur, Sanaa Ismael Abdul Jabar, A. Malik, Humaira Farooqi, Sourab Agarwal, B. Panda
The phytochemical profiling of different extracts revealed the presence of high concentration of CBD in 80:20 hydroalcoholic extract, and that of THC in 60:40 hydroalcoholic extract. The MTT assay of combination of CBD and THC highlighted the extracts as potent cytotoxic agents against prostate cancer (PC3) cells, with IC50 values of 1292 ng/ml, 953.3 ng/ml and 1134 ng/ml, respectively for HCP-CO4, HCM-CO1 & HCZ-CO1. The molecular docking study revealed a good binding of androgen receptor (PDB ID, 2am9) with CBD and THC possessing binding affinity energy of -7.1 and -7.2 kcal/mol respectively. Based on the amino acid residual interaction of CBD and THC within the 2am9 receptor, THC reported additional hydrogen bonds as compared to CBD, suggesting it to be more potent antagonist in comparison to CBD. The present study highlighted the potential of CBD and THC as a therapeutic agent for treatment of prostate cancer.
{"title":"In-silico and in-vitro studies of two cannabinoids of Cannabis sativa against prostate cancer","authors":"Jasmeet Kaur, Sanaa Ismael Abdul Jabar, A. Malik, Humaira Farooqi, Sourab Agarwal, B. Panda","doi":"10.31254/jahm.2023.9304","DOIUrl":"https://doi.org/10.31254/jahm.2023.9304","url":null,"abstract":"The phytochemical profiling of different extracts revealed the presence of high concentration of CBD in 80:20 hydroalcoholic extract, and that of THC in 60:40 hydroalcoholic extract. The MTT assay of combination of CBD and THC highlighted the extracts as potent cytotoxic agents against prostate cancer (PC3) cells, with IC50 values of 1292 ng/ml, 953.3 ng/ml and 1134 ng/ml, respectively for HCP-CO4, HCM-CO1 & HCZ-CO1. The molecular docking study revealed a good binding of androgen receptor (PDB ID, 2am9) with CBD and THC possessing binding affinity energy of -7.1 and -7.2 kcal/mol respectively. Based on the amino acid residual interaction of CBD and THC within the 2am9 receptor, THC reported additional hydrogen bonds as compared to CBD, suggesting it to be more potent antagonist in comparison to CBD. The present study highlighted the potential of CBD and THC as a therapeutic agent for treatment of prostate cancer.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. P. Verma, Md Abu Nayyer, Saudan Singh, Saba Siddiqui, D. Kumar, B. Agri, R. Padalia
Fenugreek (Trigonella foenum-graecum L.) is an important spice and medicinal herb that is widely cultivated in Uttarakhand, India. The state of Uttarakhand is known for its rich diversity of fenugreek, which provides a valuable source of genetic material for breeding programs to improve the crop's productivity, quality, and resilience. Fenugreek has a long history of use in the region, both as a food and as a medicine, and its diversity is an important part of the cultural heritage of the region. In this review, we provide an overview of the diversity of fenugreek in Uttarakhand, including its geographical distribution, genetic and morphological diversity, nutritional and medicinal properties, and traditional uses. We also discuss the threats to fenugreek diversity in the region and the importance of conserving it for food security, medicinal value, cultural heritage, and environmental sustainability. The review highlights the need for continued research and conservation efforts to ensure the preservation of fenugreek diversity in Uttarakhand.
{"title":"Exploring genetic and morphological diversity of fenugreek (Trigonella foenum-graecum L.) in Uttarakhand: A Review","authors":"P. P. Verma, Md Abu Nayyer, Saudan Singh, Saba Siddiqui, D. Kumar, B. Agri, R. Padalia","doi":"10.31254/jahm.2023.9307","DOIUrl":"https://doi.org/10.31254/jahm.2023.9307","url":null,"abstract":"Fenugreek (Trigonella foenum-graecum L.) is an important spice and medicinal herb that is widely cultivated in Uttarakhand, India. The state of Uttarakhand is known for its rich diversity of fenugreek, which provides a valuable source of genetic material for breeding programs to improve the crop's productivity, quality, and resilience. Fenugreek has a long history of use in the region, both as a food and as a medicine, and its diversity is an important part of the cultural heritage of the region. In this review, we provide an overview of the diversity of fenugreek in Uttarakhand, including its geographical distribution, genetic and morphological diversity, nutritional and medicinal properties, and traditional uses. We also discuss the threats to fenugreek diversity in the region and the importance of conserving it for food security, medicinal value, cultural heritage, and environmental sustainability. The review highlights the need for continued research and conservation efforts to ensure the preservation of fenugreek diversity in Uttarakhand.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"229 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Overweight and obesity are the commonest lifestyle disorder found in developed countries worldwide. Many a times, obesity causes life threatening complications. Simple remedies for this disease are rare. Eranda Patra Kshara is selected from Bhaishajya Ratnavali and is prepared as a remedial medicine for treating obesity. Aims and objectives: To prepare the Eranda Patra Kshara as per the classical literature, Bhaishajya Ratnavali and study its efficacy in Sthaulya [1]. Materials and Methods: Thirty patients were selected for the clinical study. One gram of Kshara along with 500 mg of Suddha Hing (asafoetida) and 50 ml of Manda (rice gruel) was given before breakfast, once daily, for 30 days. No side effects were observed in the treatment period and in follow-up period. Results: The reduction in the weight of the raw drug (i.e., original 12 kgs) when compared to that of the final product (i.e., 310 gms) was observed to be 97.42%. Statistically significant change was observed in some of the signs like nidradhikya and angagaurava. Overall effect of therapy in signs and symptoms was around 70%. Eranda Patra Kshara is Kapha hara, Medo hara and is useful in the treatment of Sthaulya.
{"title":"A Clinical study to examine the impact of Eranda Patra Kshara on Sthaulya (Obesity)","authors":"S. Sapna, Menakshi Pachori, M. Mohanan","doi":"10.31254/jahm.2023.9303","DOIUrl":"https://doi.org/10.31254/jahm.2023.9303","url":null,"abstract":"Background: Overweight and obesity are the commonest lifestyle disorder found in developed countries worldwide. Many a times, obesity causes life threatening complications. Simple remedies for this disease are rare. Eranda Patra Kshara is selected from Bhaishajya Ratnavali and is prepared as a remedial medicine for treating obesity. Aims and objectives: To prepare the Eranda Patra Kshara as per the classical literature, Bhaishajya Ratnavali and study its efficacy in Sthaulya [1]. Materials and Methods: Thirty patients were selected for the clinical study. One gram of Kshara along with 500 mg of Suddha Hing (asafoetida) and 50 ml of Manda (rice gruel) was given before breakfast, once daily, for 30 days. No side effects were observed in the treatment period and in follow-up period. Results: The reduction in the weight of the raw drug (i.e., original 12 kgs) when compared to that of the final product (i.e., 310 gms) was observed to be 97.42%. Statistically significant change was observed in some of the signs like nidradhikya and angagaurava. Overall effect of therapy in signs and symptoms was around 70%. Eranda Patra Kshara is Kapha hara, Medo hara and is useful in the treatment of Sthaulya.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"196 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The root of Berchemia lineata (L.) DC is used to treat rheumatoid arthritis in Vietnam. The chemical compounds and evaluation of the xanthine oxidase inhibitory capacity of root extracts were studied in this work. Two compounds of quercetin and emodin were the identified compounds in the roots. Rubrofusarin 6-O-α-L-rhamnosyl-(1 → 6)-O-β-D-glucopyranoside (compound 2) being new compounds was firstly identified in the roots. The compound 2 has highest xanthine oxidase enzyme inhibitory capacity compared to quercetin, emodin and E70.
{"title":"Inhibitory capacity of enzyme xanthine oxidase of extract and compounds from roots of Berchemia lineata (L.) DC","authors":"Quang Ung Le, Thi-Ngoc Lanh","doi":"10.31254/jahm.2023.9305","DOIUrl":"https://doi.org/10.31254/jahm.2023.9305","url":null,"abstract":"The root of Berchemia lineata (L.) DC is used to treat rheumatoid arthritis in Vietnam. The chemical compounds and evaluation of the xanthine oxidase inhibitory capacity of root extracts were studied in this work. Two compounds of quercetin and emodin were the identified compounds in the roots. Rubrofusarin 6-O-α-L-rhamnosyl-(1 → 6)-O-β-D-glucopyranoside (compound 2) being new compounds was firstly identified in the roots. The compound 2 has highest xanthine oxidase enzyme inhibitory capacity compared to quercetin, emodin and E70.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rohith Mohanan, Sherin Thomas, Nisaath Begum, R. Shankar, Arya Mohan, S. Sujith, A. Nisha
Scientific world is in search of newer and effective therapies against cancer and nature form a good source of drugs. The present study was undertaken to assess the antiproliferative potential of methanolic extract of A. cobbe in C127I cell lines. The leaves of A. cobbe were shade dried and was extracted using methanol and qualitative phytochemical analysis was performed. The extract was assessed for its cytotoxicity by MTT dye reduction assay in C127 I cells maintained using DMEM and 10 per cent foetal bovine serum at concentrations of 320, 160, 80, 40, 20, 20 and 5 µg/mL and the percent cell inhibition and IC50 were calculated. Acridine Orange/Ethidium bromide staining was used to detect the possible mechanism of cytotoxicity. From the results of MTT assay, it could be seen that there was a dose dependent inhibition of cell proliferation of C127I which was maximum at a concentration of 320 µg/mL. The IC50 value of the methanolic extracts was found to be 64.63 µg/mL respectively. The effect was comparable to doxorubicin. The extract and positive control treated cells showed orange to red fluorescence when stained with Acrdine Orange/ Ethidium bromide compared to greenish fluorescence in the control cells indicating apoptosis in the treated cells. The study concluded that methanolic extract of A. cobbe induced cytotoxicity by apoptosis of cancer cells.
{"title":"Antiproliferative effect of methanolic extract of Allophylus cobbe in C127I cell lines","authors":"Rohith Mohanan, Sherin Thomas, Nisaath Begum, R. Shankar, Arya Mohan, S. Sujith, A. Nisha","doi":"10.31254/jahm.2023.9203","DOIUrl":"https://doi.org/10.31254/jahm.2023.9203","url":null,"abstract":"Scientific world is in search of newer and effective therapies against cancer and nature form a good source of drugs. The present study was undertaken to assess the antiproliferative potential of methanolic extract of A. cobbe in C127I cell lines. The leaves of A. cobbe were shade dried and was extracted using methanol and qualitative phytochemical analysis was performed. The extract was assessed for its cytotoxicity by MTT dye reduction assay in C127 I cells maintained using DMEM and 10 per cent foetal bovine serum at concentrations of 320, 160, 80, 40, 20, 20 and 5 µg/mL and the percent cell inhibition and IC50 were calculated. Acridine Orange/Ethidium bromide staining was used to detect the possible mechanism of cytotoxicity. From the results of MTT assay, it could be seen that there was a dose dependent inhibition of cell proliferation of C127I which was maximum at a concentration of 320 µg/mL. The IC50 value of the methanolic extracts was found to be 64.63 µg/mL respectively. The effect was comparable to doxorubicin. The extract and positive control treated cells showed orange to red fluorescence when stained with Acrdine Orange/ Ethidium bromide compared to greenish fluorescence in the control cells indicating apoptosis in the treated cells. The study concluded that methanolic extract of A. cobbe induced cytotoxicity by apoptosis of cancer cells.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77886178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayurveda prophesizes that living in tune with nature's cycles is one of the keys to good health. All living things have a circadian clock which keeps track of night and day in the brain, orchestrates our daily rhythms of physiological, behavioural and molecular processes, and plays an explicit role in the immune system. This cycle regulates cytokine production and the activity of immune cells, such as T cells and B cells, which makes it closely intertwined with our immune system.
{"title":"Role of the Ayurvedic clock in boosting the immune system","authors":"Punyakishore Maibam","doi":"10.31254/jahm.2023.9208","DOIUrl":"https://doi.org/10.31254/jahm.2023.9208","url":null,"abstract":"Ayurveda prophesizes that living in tune with nature's cycles is one of the keys to good health. All living things have a circadian clock which keeps track of night and day in the brain, orchestrates our daily rhythms of physiological, behavioural and molecular processes, and plays an explicit role in the immune system. This cycle regulates cytokine production and the activity of immune cells, such as T cells and B cells, which makes it closely intertwined with our immune system.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82042938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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