中药材最新文献

Objective: To study the chemical constituents from fruiting bodies of Postia balsamea.

Methods: The fruiting bodies of Postia balsamea was extracted,the compounds were isolated by silica gel column chromatography. The structures were elucidated by the spectral analysis.

Results: Seven compounds were isolated and identified as botulin ( 1),( 22 E,24R)-5α,8α-epidioxy-ergosta-6,22-dien-3β-ol( 2),( 22 E,24R)-5α,8α-epidioxy-ergosta-6,9( 11),22-trien-3β-ol( 3),( 22 E,24R)-ergosta-7,22-dien-3β-ol( 4), betulinic acid( 5),( 22 E,24R)-ergosta-5,7,22-trien-3β-ol ( 6) and stearic acid( 7).

Conclusion: All the compounds are isolated from this species for the first time.

Objective: To develop a sensitive and reliable ultra performance liquid chromatography tandem mass spectrometry ( UPLC-MS / MS) method for simultaneous determination and pharmacokinetics of protocatechuic acid, kaempferol biotin glucoside and quercitrin in rat plasma, and study their pharmacokinetic characteristics in rats.

Methods: Three compounds were simultaneously determined by UPLC-MS / MS on Waters BEH C18( 50 mm × 2. 1 mm,1. 7 μm) column. The mobile phase was 0. 1% acetonitrile formic acid and0. 1% aqueous formic acid, and programmed in a linear gradient mode. The compounds were ionized in the electrospray ionization ion source of the mass spectrometer and detected in MRM mode.

Results: The t1 /2of protocatechuic acid,kaempferol biotin glucoside and quercitrin after intravenous injection were( 41. 9 ± 12. 3),( 71. 3 ± 56. 8) and( 90. 3 ± 74. 8) min. The Cmaxwere( 1. 7 ± 0. 7) μg / m L,( 2. 1 ± 0. 9) μg/m L,( 8. 7 ± 3. 7) μg/m L.

Conclusion: The established method is specific,rapid,accurate and sensitive,and is proved to meet the requirements of biological sample analyses,and is suitable for the concentration determination of protocatechuic acid, kaempferol biotin glucoside and quercitrin in rat plasma, three compounds are all best fitted to the two-compartment open pharmacokinetic model.

Objective: To investigate the effects of gambogenic acid on proliferation,apoptosis and invasion of human cervical carcinoma HeLa cells.

Methods: HeLa cells were given different concentrations of gambogenic acid( 0. 00,0. 63,1. 25,2. 50,5. 00 and 10. 00 mg / L) for 72 h,MTT assay was used to measure the cells proliferation. Flow cytometry was used to measure cell cycle. Fluorescence microscopy and DNA ladder analysis were used to measure the apoptosis of HeLa cells. Transwell chambers was adopted to detect the cells invasion. The expression levels of mRNA and protein of BCL-2,BAX,NF-κB and E-cadherin were detected by RT-PCR and Western blot.

Results: After treatment with different concetrations of gambogenic acid for 72 h, the proliferation of HeLa cells was significantly inhibited, and in a concentration dependent manner. The ability of invasion was decreased with the concentration of gambogenic acid increased, which was detected by Transwell chamber assays in vitro. RT PCR and Western blot demonstrated that gambogenic acid up-regulated the expressions of BAX and E-cadherin and down-regulated the expression of mRNA and protein of BCL-2 and NF-κB.

Conclusion: Gambogenic acid can inhibite the proliferation and invasion of HeLa cells,and promote the cells apoptosis in a concentration dependent manner.

Objective: On the basis of molecular docking,to study the mechanism of Panax ginseng in the treatment of ischemic stroke.

Methods: The small molecules of Panax ginseng based on molecular docking technology docked with 20 key targets protein of cerebral ischemia, and multi-component protein target network was established by Cytoscape 3. 1. 1 software. At the same time,the two active molecules of the Panax ginseng of Rb2 and 20( R)-ginsenoside Rg2 were also analyzed by VEGF and Caspase-3,which were the key protein in brain ischemia.

Results: There were 31 active molecules of Panax ginseng combined strongly with five or more than five protein targets after molecular docking, and only four active molecules of Panax ginseng combined strongly with ten or more than ten protein targets. The two active molecules of Rb2 and 20( R)-ginsenoside Rg2 in the Panax ginseng had a very strong combination with VEGF and Caspase-3,respectively,and the docking scores were more than 7. 0.

Conclusion: Molecular docking technology screening active substances of Panax ginseng plays a practical significance in the treatment of ischemic stroke, which offers the foundation to study the chemical constituents of composite prescription.

Objective: To optimize the preparation of total saponins of Aralia taibaiensis phospholipid complex( TSAT-PC) by the central composite design-response surface method.

Methods: Total saponins of Aralia taibaiensis phospholipid complex was prepared by using solvent evaporation method, five factors including reaction solvent, reaction time, reaction temperature, ratio of reactants on this reaction, and the concentration of the drug were investigated, then to optimize the preparation of TSAT-PC by the central composite design response surface method, and to study its physicochemical properties.

Results: The optimal process conditions were as follows, the reaction time was 1 h, the reaction temperature was 45 ℃,the ratio of soya lecithine ( SL) and TSAT was 3∶ 1, the reaction concentration was16 mg / m L, the complexing rate was 97. 23%,it was less than 5% with the predicted deviation; IR analysis proved the formation of TSAT-PC, and the solubility in the octyl alcohol was higher than the original drug.

Conclusion: TSAT-PC was successfully developed by the optimized process, enhance the solubility in octyl alcohol, which provide the reference for the further development and utilization of Chinese materia medica preparation.

Objective: To study the relationship between the component content of curcuminoids in Curcumae Longae Rhizoma and powder color indeces L*, a* and b* those were measured by chromaticity instrument, in order to provide scientific basis for quality assessment of Curcuma longa medicinal materials.

Methods: Detect the content of curcumin,demethoxycurcumin,bisdemethoxycurcumin,then detect the content of curcuminoids by the Chinese Pharmacopoeia method. Measure the color indeces L*, a* and b* of Curcumae Longae Rhizoma powder by colorimeter. Finally, the relationship between the content and the color was analyzed by using the Grey Relational Analysis, Pearson correlation coefficient and regression analysis.

Results: There was positive correlation between the content of curcuminoids and a*. But there was no definitely relation between the content of curcuminoids and b* or L*.

Conclusion: The content of curcuminoids is closely related with the degree of the color red, the higher the content, the red is deeper. a* could be recognized as an important basis of Curcumae Longae Rhizoma medicinal materials quality.

Objective: To explore the preventive effects of Jiawei Yinchen Sini Decoction on the gene expression of Smad7 and Collagen,Ⅲ in mice with hepatic fibrosis.

Methods: 48 male ICR mice were randomly divided into normal group, model group, colchicine group, JWYSN high dose group, medium dose group and low dose group, with 8 mice in each group. The hepatic fibrosis model was established by intraperitoneal injection of 30% CCl4( 1. 5 mg/kg, dissolved in olive oil). At the same time, the mice were treated with intragastric adminstration of drugs. After 14 days, the liver index was calculated; the levels of serum hyaluronic acid( HA) and laminin protein( LN) were detected by Enyme-linked immunosorbent assay; the pathological fibrosis change was observed by performing HE staining and was made a grading of hepatic fibrosis. mRNA expression of Smad7 and Collagen I, Ⅲ were detected by RT-q PCR analysis.

Results: (1) Compared with model group, the liver index and levels of HA and LN in JWYSN medium dose group and low dose group were obviously decreased( P < 0. 05).( 2) The pathological results showed that, compared with model group, the hepatic fibrosis were significantly relief in different dose of JWYSN group( P < 0. 05).( 3) The expressions of Collagen Ⅰ mRNA in each treatment group were significantly lower than model group( P < 0. 05); In addition to JWYSN high dose group, the expressions of Smad7 mRNA in each treatment group increased significantly( P < 0. 05); In addition to JWYSN low dose group, the expression of CollagenⅢmRNA in each treatment group decreased significantly( P < 0. 05).

Conclusion: Jiawei Yinchen Sini decoction can up-regulate the expressions of Smad7 mRNA and inhibit expressions of mRNA and CollagenⅢmRNA in mice with hepatic fibrosis,thus attenuate the development of hepatic fibrosis.

Objective: To study the chemical constituents of Litchi chinensis pericarp.

Methods: The compounds were isolated by Sephadex LH-20, MCI gel CHP 20 P, Toyopearl Butly-650 C, Toyopearl WH-40 F column chromatographies and semi-preparative HPLC, the structures were elucidated by NMR and mass spectroscopic MS data analysis.

Results: 14 compounds were obtained and their structures were identified as quercetin( 1), chrysoeriol( 2),kaemperol-3-O-β-D-glucoside( 3), manghaslin( 4), isorhamnetin-3-O-robinobioside( 5), ( +)-gallocatechin( 6), (-) epicatechin-3-O-gallate( 7), cinnamtannin B-1( 8), isolariciresinol-9-O-β-D-xyloside( 9), ( +)-5-methoxyisolariciresinol-9-O-β-D-xylopyranoside( 10), vanillic acid( 11), 3, 4, 3’, 4’-tetrahydroxy biphenyl( 12), tachioside( 13) and isotachioside( 14).

Conclusion: Compounds 1 ~ 7,9 ~ 14 are obtained from this plant pericarp for the first time.

Objective: To study the chemical constituents of Swertia binchuanensis.

Methods: The constituents were separated and purifyed by conventional methods. Their structures were identified on the basis of spectral analysis such as 1H-NMR,13C-NMR.

Results: Seven compounds were isolated and identified as 1, 2, 3, 4-tetrahydro-1, 4, 8-trihydroxy-6-methoxyxanhone（ Ⅰ）, erythrocentaurin（ Ⅱ）,1-O-β-D-glucopyranosyl-1, 2, 3, 4-tetrahydro-4, 8-dihydroxy-6-methoxyxanthone（ Ⅲ）, maslinic acid（ Ⅳ）,1-hydroxy-3, 7-dimethoxyxanthone（ Ⅴ）,1, 8-dihydroxy-3, 5-dimethoxyxanthone（ Ⅵ）,1, 7-dihydroxy-3, 4, 8-termethoxyxanthone（ Ⅶ）.

Conclusion: All compounds were isolated from Swertia binchuanensis for the first time.

Objective: To investigate the chemical constituents from stems of Kadsura longipedunculata.

Methods: The constituents were isolated and purified by various chromatographic methods,and their structures were elucidated by spectroscopic analysis and comparison with literatures.

Results: Seven compounds were isolated from the stems of Kadsura longipedunculata and were identified as longipedunin D( 1),renchangianin A( 2),renchangianin B( 3),meso-dihydroguaiaretic acid( 4),isolariciresinol-9-O-β-D-xyloside( 5),(-)-gallocatechin( 6) and( +)-catechin( 7).

Conclusion: Compound 1 is a novel lignan,and compounds 2,3 are isolated from this plant for the first time.