中药材最新文献

Objective: To investigate the active components and potential mechanism of Puerariae Radix in improving insulin resistance by using network pharmacological method.

Methods: Key target proteins related with insulin resistance were selected based on molecular docking technology, and then took the selected components with 31 target proteins of four pathways for docking. Meanwhile, component-target proteins network was established to network analysis by software Cytoscape 3. 2. 1.

Results: 19 compounds had close interactions with four pathways such as AMPK. There were 13 compositions can verify through literature, which revealing that active ingredients and potential molecular mechanism of Puerariae Radix in improving insulin resistance, preliminarily.

Conclusion: The network pharmacological method is helpful to explore the possible active components in Puerariae Radix and elucidate the mechanism.

Objective: To provide the experimental evidence for the appropriate selection of the different prepared products from Gardenia jasminoides fruits by comparing their protection effects on carbon tetrachloride（ CCl4）-induced acute liver injury.

Methods: The activities of ALT,AST,ADA,LDH,ALP and contents of PA,TP,TBIL,DBIL,TBA in serum,the activities of SOD and the content of MDA in liver tissue were measured in acute liver injury rats by carbon tetrachloride. Also the pathological changes of liver tissues were examined under microscope.

Results: The biochemical indexes of AST,ALT,TBA,ADA,LDH and MDA were significantly improved in all groups of prepared products from Gardenia jasminoides fruits,but not SOD and ALP. The lesions of liver tissue had different degrees of reduction.

Conclusion: The different prepared products from Gardenia jasminoides fruits had the effects of liver protection. The nut of Gardeniae Fructus was superior to the peel in enzyme decreasing and liver protection. The crude was superior to the stir-cooked in enzyme decreasing and liver protection.

Objective: To investigate the chemical constituents of Clematis aethusifolia,a traditional Mongolian medicine for resolving hard lump.

Methods: Bioactive guided isolation and purification of Clematis aethusifolia was performed by normal and reverse phase column chromatography, Sephadex LH-20 and preparation HPLC. The structures were identified by 1D,2D-NMR and MS spectral analysis and comparison with literature data. Cytotoxicity of compounds were determined by CCK-8 cell staining method for detecting growth inhibition to five kinds of human solid tumor cell lines.

Results: Eight compounds were isolated and identified as vanillicacid( 1),protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity.

Conclusion: All the compounds are isolated from this plant for the first time, Results: Eight compounds were isolated and identified as vanillicacid( 1), protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4’-O-β-D-glucopyranoside( 4),( +)-syringaresinol-4’-O-β-Dglucopyranoside( 5),7,9,9’-trihydroxy-3,3’-dimethoxy-8-O-4’-neolignan-4-O-β-D-glucopyranoside( 6),butyl-β-D-fructopyranoside( 7),butyl-β-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity. compounds 3,6 ~ 8 are isolated from Clematis genus for the first time, compounds 6 and 8 are isolated from Ranunculaceae family for the first time. Compounds 2,8 may be the effective components in Clematis aethusifolia.

Objective: On the basis of previous studies, to construct a genetic map of Salvia miltiorrhiza by using EST-SSR primers, to build a platform for positioning important traits relating to genes, the cloning and molecular marker-assisted in breeding of new varieties of Salvia miltiorrhiza.

Methods: A total of 411 EST-SSR primers were used for PCR amplification to screen polymorphic markers in F1 mapping population derived from a cross between Salvia miltiorrhiza, two parents of which were the cultivars of ZH74 and BH18. Combined with the molecular marker data of previous studies, Joinmap 4. 0 software was used for map integration.

Results: 411EST-SSR primers were screened from two parents, a total of 328 pairs of primers were amplified stable products,164 pairs of polymorphic primers were obtained.

Conclusion: A linkage map of Salvia miltiorrhiza with 150 marker high density genetic is constructed.

Objective: To breed new Rehmannia glutinosa varieties with best comprehensive properties.

Methods: By space mutation of hybrid seeds of 85-5 and Beijing No. 1,after systemic breeding, Huaidi 81 with excellent comprehensive characters was screened. Fresh weight, benchmark composition content, resistance, chlorophyll and anthocyanin content, photosynthetic characteristics of the new variety and the main cultivars were determined.

Results: Per plant fresh weight of Huaidi 81 was higher than those five main varieties and there was extremely significant difference between Huaidi 81 and other varieties,the yield of Huaidi 81( 82 353 kg / hm2) was29. 7% higher than that 5 main varieties. The catalpol content of different Rehmannia glutinosa varieties ranked in the order as follows, Beijing No. 3( 1. 601%) > Qinhuai( 1. 588%) > Huaidi 81( 1. 314%) > Godden nine > 85-5( 1. 073%) > Huaifeng( 0. 924%),the catalpol content of Huaidi 81 was in the middle, and there was no significant difference between Huaidi 81 and Godden nine,but extremely significant difference from other varieties. The acteoside content of different Rehmannia glutinosa varieties ranked in the order as follows,Huaidi 81( 0. 096%) > Qin Huai( 0. 069%) > 85-5( 0. 047%) > Beijing No. 3( 0. 035%) > Huaifeng( 0. 023%) > Golden nine( 0. 022%),the content of acteoside of Huaidi 81 was higher than those five main varieties, and there was extremely significant difference between Huaidi 81 and other varieties. Huaidi 81 had high resistance to Septoris digitalis, and had middle resistance to leaf ring rot, which indicated that Huaidi 81 had good resistance to leaf disease. Huaidi 81 with the highest chlorophyll content and moderate anthocyanin content, and had the highest photosynthetic rate.

Conclusion: The new variety Huaidi 81 with best comprehensive properties is suitable for popularizing as a new Rehmannia glutinosa variety.

Objective: To study the chemical constituents from the fruit of Swietenia macrophylla.

Methods: The chemical constituents were extracted and isolated by silica gel, Sephadex LH-20. The chemical structures of components were further elucidated by the physicochemical characters and MS,NMR spectral data.

Results: 17 compounds were isolated and identified as swietenine( 1),swietenine acetate( 2),febrifugine( 3),khayasin T( 4),3-O-tigloyl-6-O-acetylswietenolide( 5),3-O-tigloylswietenolide( 6),3,6-O,O-diacetylswietenolide( 7),fissinolide( 8),3-O-acetylswietenolide( 9), proceranolide( 10),swietenolide( 11),swietemahonin E( 12),swietemahonin F( 13),7-deacetoxy-7-oxogedunin( 14),secomahoganin( 15),altissimanin B( 16),ororatone( 17).

Conclusion: Compounds3,5,8 ~ 10,16,17 are isolated from this plant for the first time.

Objective: To investigate the effect and mechanism of berberine in attenuating PM2. 5-induced human umbilical vein endothelial cells EA. hy926 injury.

Methods: The samples of fine particulate matter( PM2. 5) were collected and made into suspension. Different concentrations of PM2. 5( 0,20,200,400 mg / L) were added into EA. hy926 cells for 24 h. The viability of EA. hy926 cells was detected by MTT assay, and apoptosis of EA. hy926 cells was detected by flow cytometry, the expressions of p-ERK1 /2,BAX and BCL-2 in the EA. hy926 cells were measured by Western blot,the contents of IL-6,TNF-α were measured by ELISA, the content of MDA, and the activities of SOD and LDH in the EA. hy926 cells culture supernatant were measured, respectively. Different concentrations of berberine( 10,50,100 μmol / L) and PD98059( 20 μmol / L) was added into the EA. hy926 cells to observe the effect of berberine.

Results: Compared with control group,PM2. 5 decreased the viability in a dose dependent manner, and significantly upgraded the protein levels of p-ERK1 /2 and BAX / BCL-2 ratio,PM2. 5 increased the apoptosis of EA. hy926 cells, and increased the contents of IL-6,TNF-α and MDA, increased the activity of LDH, and decreased SOD activity in the EA. hy926 cells( P < 0. 05). Compared with PM2. 5 group, berberine increased the viability of EA. hy926 cells in a dose dependent manner,PM2. 5 significantly downgraded the protein levels of p-ERK1 /2 and BAX / BCL-2 ratio, and decreased the apoptosis of EA. hy926 cells, decreased the contents of IL-6,TNF-α and MDA, and decreased the activity of LDH, and increased SOD activity in the EA. hy926 cells( P < 0. 05).

Conclusion: Berberine attenuates PM2. 5-induced EA. hy926 cells injury by inhibiting ERK1 /2 pathway.

Objective: To investigate the effect of Dahuang-Taoren with different proportion of extraction amount changes of ten kinds of chemical constituents in Rhizoma Rhei.

Methods: Uniform method to set different ratio( 1∶ 5,2∶ 5,2∶ 3,1∶ 1,3∶ 2,5∶ 2,5∶ 1),and set the control group ( Dahuang-Taoren( 1 ∶ 0). HPLC was used to determine the content of ten constituents as gallic acid,( +)-catechin,sennoside B,anthraquinones( aloe-emodin,rhein,emodin,chrysophanol,physcion,chrysophanol-1-O-glucoside,emodin major grape glycoside) in different ratio of drug. Different proportions of Dahuang-Taoren on the extraction amount of ten chemical components in Rhizoma Rhei changes were analyzed. .

Results: Compared to the control group, Dahuang-Taoren with different ratio( 5∶ 1,5∶ 2,3∶ 2) in a sample with increasing proportion of Taoren,the extraction amount of ten kinds of constituents of Rhizoma Rhei gradually decreased;Dahuang-Taoren with ratio of 1∶ 1,ten kinds of constituents in extraction of total amount arrived minimum value. Dahuang-Taoren with different ratio( 2∶ 3,2∶ 5,1∶ 5) in a sample with increasing proportion of Taoren,the extraction amount of gallic acid,( +) catechin,chrysophanol of Rhizoma Rhei increased significantly.

Conclusion: There is obvious change in chemical constituents of the extraction amount of Rhizoma Rhei with the change of the ratio in Dahuang-Taoren, and Dahuang-Taoren with the ratio( 2∶ 3,2∶ 5,1∶ 5),the extraction amount of gallic acid,( +)-catechin,sennoside B,aloe-emodin,emodin,chrysophanol,physcion,chrysophanol-1-O-glucoside are significantly higher than control group.

Objective: To study the rapid propagation in vitro of Dioscorea opposita‘Guangfeng’, and to observe the stomas of the transplanting plantlets and potted seedlings, to test chromosome ploidy by FCM, and to detect DNA mutation by ISSR,in order to provide the technical basis for the large-scale production of Dioscorea opposita ‘Guangfeng’ plantlets.

Methods: The technique system of Dioscorea opposita ‘Guangfeng’rapid propagation in vitro was established and optimized by plant tissue culture method. The parameters of transplanting plantlets and potted seedlings were studied as follows, the stomatal parameters were observed by transparent adhesive tape method, chromosome ploidy were analyzed by FCM, and DNA mutation were detected by ISSR molecular marker.

Results: The technique system of Dioscorea opposite ‘Guangfeng’ rapid propagation in vitro was as follows, slightly woody stem segment with a bud were selected and inoculated onto MS + KT 1 mg / L + NAA 0. 2 mg / L solid culture medium and cultured in the photoperiod of 14 h / d( the temperature was( 25 ± 2) ℃ and light intensity was 1 500 ~ 2 000 Lx) after disinfected for 1 min in 70% alcohol prior to sterilized for 12 min with 0. 1% Hg Cl2,the materials were washed with sterile water for 3 times, respectively. The new bud was cut off when it grew to 2 ~ 3cm and inoculated into MS + KT 2 mg / L + NAA 0. 5 mg / L liquid culture medium and continued to culture in above culture conditions. The whole plant was formed after cultured for about 90 d. The sealing membrane was opened in transplanting, and the plantlets was still placed in above culture conditions and cultured for 2 ~ 3 d, and then the whole plant was taken out, and the culture medium washed off and then transferred into the vessel with shallow liquid MS basic culture medium and domesticated indoor. The acclimated plantlets were taken out and transplanted in the outdoor pots with the sandy soil when the new shoots grew out, and watered one time with tap water in the morning and evening per day, the survival rate reached 100%. The results of stomatal observation, FCM analysis and ISSR detection of transplanting plantlets and potted seedlings showed that the stomatal parameters, chromosome ploidy and DNA mutation of plantlets and potted seedlings had no variation.

Conclusion: The results reveal that the establishment and optimization of the technique system of Dioscorea opposita ‘Guangfeng’ rapid propagation in vitro is feasible, and the regenerated plants do not have genetic variation which can ensure the stability of the genetic.

Objective: To study Jianpi Qingre Huoxue decoction( JPQRHX) in preventing colon cancer by observing SW480 cells proliferation,apoptosis,cycle and the expression of P-β-catenin, and to research its mechanism.

Methods: SW480 cells were incubated with serum containing blank serum, different concentrations of JPQRHX decocition and PI3 K blocking agent LY294002 for 24 h,respectively. Cell proliferation was detected by MTT assay, cell cycle and apoptosis were detected by flow cytometry. The protein translocation of P-β-catenin was assayed by immunofluorescent staining.

Results: The inhibitory rate and apoptosis rate in JPQRHX decoction group were higher than control group( P < 0. 05),respectively. S phase cells were increased significantly, and G1 phase cells and LY294002 group cells were decreased significantly( P < 0. 05). The P-β-catenin in JPQRHX decoction groups were mainly expressed in membrane, while the P-β-catenin in the control group was characterized by deletion in membrane and increased in nucleus.

Conclusion: JPQRHX decoction has the ability in curing colon cancer, and the mechanism is associated with altering the expression of P-β-catenin in the cells nucleus, blocking SW480 cells cycle at G1 phase, inhibiting SW480 cells proliferation, and inducing SW480 cell apoptosis.