A. Fadeyibi, K. Alabi, Mary Fadeyibi, Taiwo Olusola Asaniyi
In this research, a maize starch-peanut shell nanocomposite film was applied to package mushroom under varying moisture, thickness, and cold temperature conditions. The film was developed by congealing 18 g starch, 0.38 g peanut nanoparticles, 16 g glycerol in 300 ml distilled water at 70oC, and its thermal, mechanical, barrier and microstructural behaviors were determined using standard methods. The film was applied to package oyster mushroom by varying the thickness (5– 10 mm) and moisture content (77.18 –91.14 %, wb) of the product, and thereafter storing it under 4– 8 oC cold temperature condition. The results revealed ~ 5% weight fraction degradation at ≤ 310 oC with endothermic peaks occurring at 250 oC and 400 oC, which corresponds to phase transition points where the film was thermally stable. The deformation pattern of the film at atomic level mimics a natural plastic material, with a heterogeneous particle size distribution across the film matrix. The permeability coefficients were 0.68× 10-10, 2.10× 10-10 and 14.0× 10-10 cm3 (STP) cm/cm2scm Hg for nitrogen, oxygen, and carbon-dioxide, gases, respectively. Also, the microbial load of the packaged product significantly decreased with an increase pH, moisture, and temperature (p< 0.05). Thus, the film can be suitable for mushroom packaging.
{"title":"APPLICATION OF MAIZE STARCH-PEANUT SHELL NANOCOMPOSITE PACKAGING ON MUSHROOM UNDER VARYING MOISTURE, THICKNESS, AND COLD STORAGE","authors":"A. Fadeyibi, K. Alabi, Mary Fadeyibi, Taiwo Olusola Asaniyi","doi":"10.55251/jmbfs.6071","DOIUrl":"https://doi.org/10.55251/jmbfs.6071","url":null,"abstract":"In this research, a maize starch-peanut shell nanocomposite film was applied to package mushroom under varying moisture, thickness, and cold temperature conditions. The film was developed by congealing 18 g starch, 0.38 g peanut nanoparticles, 16 g glycerol in 300 ml distilled water at 70oC, and its thermal, mechanical, barrier and microstructural behaviors were determined using standard methods. The film was applied to package oyster mushroom by varying the thickness (5– 10 mm) and moisture content (77.18 –91.14 %, wb) of the product, and thereafter storing it under 4– 8 oC cold temperature condition. The results revealed ~ 5% weight fraction degradation at ≤ 310 oC with endothermic peaks occurring at 250 oC and 400 oC, which corresponds to phase transition points where the film was thermally stable. The deformation pattern of the film at atomic level mimics a natural plastic material, with a heterogeneous particle size distribution across the film matrix. The permeability coefficients were 0.68× 10-10, 2.10× 10-10 and 14.0× 10-10 cm3 (STP) cm/cm2scm Hg for nitrogen, oxygen, and carbon-dioxide, gases, respectively. Also, the microbial load of the packaged product significantly decreased with an increase pH, moisture, and temperature (p< 0.05). Thus, the film can be suitable for mushroom packaging.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48685368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vietnam is a country that produces a variety of agricultural products, including vegetables, tubers, fruits, and processed products. Along with the increase in population, the demand for consumers also increases, and the by-products of farming are increasing and being discharged into the environment. This is one of the critical research issues that need to be solved to ensure sustainability in agriculture. This review summarized recent studies on familiar sources of by-products in Vietnam, such as banana peels, citrus peels, dragon fruit skins, rice bran, and rice husks, and their potential in the food industry. Some solutions are also proposed to solve and turn this low-value raw material into a high-value product and serve a variety of products and consumers in the food industry. Especially after the COVID-19 pandemic, the by-products contain valuable and reusable biological resources. These compounds could be future applications to support improving the consumer's immune system and various health benefits. Processed and utilized by-products from food production could not only help increase incomes for farmers, especially in developing countries like Vietnam but also could aid in ensuring food security and sustainability in agricultural production.
{"title":"FOOD PROCESSING WASTE IN VIETNAM: UTILIZATION AND PROSPECTS IN FOOD INDUSTRY FOR SUSTAINABILITY DEVELOPMENT","authors":"Ngo Van Tai, V. Minh, N. Thuy","doi":"10.55251/jmbfs.9926","DOIUrl":"https://doi.org/10.55251/jmbfs.9926","url":null,"abstract":"Vietnam is a country that produces a variety of agricultural products, including vegetables, tubers, fruits, and processed products. Along with the increase in population, the demand for consumers also increases, and the by-products of farming are increasing and being discharged into the environment. This is one of the critical research issues that need to be solved to ensure sustainability in agriculture. This review summarized recent studies on familiar sources of by-products in Vietnam, such as banana peels, citrus peels, dragon fruit skins, rice bran, and rice husks, and their potential in the food industry. Some solutions are also proposed to solve and turn this low-value raw material into a high-value product and serve a variety of products and consumers in the food industry. Especially after the COVID-19 pandemic, the by-products contain valuable and reusable biological resources. These compounds could be future applications to support improving the consumer's immune system and various health benefits. Processed and utilized by-products from food production could not only help increase incomes for farmers, especially in developing countries like Vietnam but also could aid in ensuring food security and sustainability in agricultural production.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44258786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucia Klongová, Matúš Kyseľ, Veronika Fialková, Eloy Fernández-Cusimamani, Adam Kováčik, J. Žiarovská
Arachis hypogaea L. – peanut (syn. groundnut) belong botanically to annual herbs, more specifically to legumes (family Fabaceae). Cultivated from is characterized as allotetraploid specie and that has a large and genome with high level of complexity as a result of natural hybridization of wild diploid species Arachis cardenasii (nn) and Arachis batizocoi. Individual analysis that are aimed to description of the genetic similarity and variability of wild and cultivated Arachis species are substantial for the knowledge of intaspecific relationships. In this study, conserved DNA-derived polymorphism technique was used to analyse genetic variability of natural genotypes of peanut. CDDP is a simple PCR based method that was proved to be efficient in the studies of plant populations polymorphism. Primers used in CDDP target conserved sequences of plant functional genes that are involved in response to abiotic and biotic stresses. Here, bulked DNA samples of twenty-one natural populations were analysed by with five CDDP primer combinations. Selected primers produced a total of 260 amplicons, among which 107 (41.2%) amplicons were polymorphic. The average number of obtained amplicons per primer was about 51.8. Amplified polymorphic fragments of analysed genotypes ranged from 13 to 33 and percentage of polymorphism ranged from 35 % to 47 %. UPGMA cluster analysis was performed on the base of prepared binary matrices and obtained clustering has grouped the analyzed peanut populations into five major groups with further subgroups. The results of CCDP fingerprinting shown, that this marker technique provide sufficient distinguishing of a wild population of Arachis hypogaea L.. Analysed genotypes of peanut were separated into different groups based on genetic diversity, however, the level of polymorphisms that detected used markers among populations of Arachis hypogaea L. was low.
{"title":"UTILIZATION OF CDDP MARKERS IN ANALYSIS OF GENETIC VARIABILITY OF ARACHIS HYPOGAEA L.","authors":"Lucia Klongová, Matúš Kyseľ, Veronika Fialková, Eloy Fernández-Cusimamani, Adam Kováčik, J. Žiarovská","doi":"10.55251/jmbfs.9884","DOIUrl":"https://doi.org/10.55251/jmbfs.9884","url":null,"abstract":"Arachis hypogaea L. – peanut (syn. groundnut) belong botanically to annual herbs, more specifically to legumes (family Fabaceae). Cultivated from is characterized as allotetraploid specie and that has a large and genome with high level of complexity as a result of natural hybridization of wild diploid species Arachis cardenasii (nn) and Arachis batizocoi. Individual analysis that are aimed to description of the genetic similarity and variability of wild and cultivated Arachis species are substantial for the knowledge of intaspecific relationships. In this study, conserved DNA-derived polymorphism technique was used to analyse genetic variability of natural genotypes of peanut. CDDP is a simple PCR based method that was proved to be efficient in the studies of plant populations polymorphism. Primers used in CDDP target conserved sequences of plant functional genes that are involved in response to abiotic and biotic stresses. Here, bulked DNA samples of twenty-one natural populations were analysed by with five CDDP primer combinations. Selected primers produced a total of 260 amplicons, among which 107 (41.2%) amplicons were polymorphic. The average number of obtained amplicons per primer was about 51.8. Amplified polymorphic fragments of analysed genotypes ranged from 13 to 33 and percentage of polymorphism ranged from 35 % to 47 %. UPGMA cluster analysis was performed on the base of prepared binary matrices and obtained clustering has grouped the analyzed peanut populations into five major groups with further subgroups. The results of CCDP fingerprinting shown, that this marker technique provide sufficient distinguishing of a wild population of Arachis hypogaea L.. Analysed genotypes of peanut were separated into different groups based on genetic diversity, however, the level of polymorphisms that detected used markers among populations of Arachis hypogaea L. was low.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43468730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Zolfaghari, Arezou Khezerlou, Seyed Alireza Banihashemi, M. Tavassoli, A. Ehsani
Access to healthy food, without any undesirable contamination, is one of fundamental human right. Some of mycotoxins, especially aflatoxins (AFs), contamination feed and food that cause problems such as acute damage liver, irritation, and cancer of the liver and teratogenic complications. Among the physical, chemical and biological methods used to prevent the production, reduction, elimination and deactivation of AF in contaminated food, biological methods have been considered, due to maximum efficiency, low cost, eco-friendly and non-degradation of nutritional quality. The protective effect of lactic acid bacteria as probiotic microorganisms against mutagenic factors, such as polycyclic amines, N-nitrosamine compounds, and mycotoxins has been proven. Therefore, in addition to the beneficial properties using these probiotic bacteria with the ability to remove AF can help to enhance food safety. Although some naturally-occurring bacteria in the intestine can be attached to harmful components such as toxins and prevent them from binding to the intestinal layer, but probiotics have the potential to inactivate toxins through surface binding, due to high adhesion properties in their cell wall proteins. Polysaccharides and peptidoglycans in the cell wall are two of the main ingredients for decomposition, bonding and binding of AFs to lactic acid bacteria. Therefore, this review examines the economic and health impacts of AF contamination in foods. Further, this review discusses how lactic acid bacteria are able to detoxify common food AFs.
{"title":"REVIEW ON BIO-DETOXIFICATION OF AFLATOXINS BASED ON LACTIC ACID BACTERIA: MECHANISM AND APPLICATIONS","authors":"H. Zolfaghari, Arezou Khezerlou, Seyed Alireza Banihashemi, M. Tavassoli, A. Ehsani","doi":"10.55251/jmbfs.9242","DOIUrl":"https://doi.org/10.55251/jmbfs.9242","url":null,"abstract":"Access to healthy food, without any undesirable contamination, is one of fundamental human right. Some of mycotoxins, especially aflatoxins (AFs), contamination feed and food that cause problems such as acute damage liver, irritation, and cancer of the liver and teratogenic complications. Among the physical, chemical and biological methods used to prevent the production, reduction, elimination and deactivation of AF in contaminated food, biological methods have been considered, due to maximum efficiency, low cost, eco-friendly and non-degradation of nutritional quality. The protective effect of lactic acid bacteria as probiotic microorganisms against mutagenic factors, such as polycyclic amines, N-nitrosamine compounds, and mycotoxins has been proven. Therefore, in addition to the beneficial properties using these probiotic bacteria with the ability to remove AF can help to enhance food safety. Although some naturally-occurring bacteria in the intestine can be attached to harmful components such as toxins and prevent them from binding to the intestinal layer, but probiotics have the potential to inactivate toxins through surface binding, due to high adhesion properties in their cell wall proteins. Polysaccharides and peptidoglycans in the cell wall are two of the main ingredients for decomposition, bonding and binding of AFs to lactic acid bacteria. Therefore, this review examines the economic and health impacts of AF contamination in foods. Further, this review discusses how lactic acid bacteria are able to detoxify common food AFs.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44006557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miroslava Hlebová, Dominika Veseleniyova, Zuzana Šrámková, Veronika Vojcikova, A. Kováčik, J. Čuboň, Ivana Charousová, Lenka Hutárová, Lukáš Hleba
This study aimed to evaluate the antifungal activity and the potential synergistic activity of ten plant extracts (EXs) and ten essential oils (EOs) obtained from the same plant species, namely Agrimonia eupatoria L., Arctium lappa L., Arnica montana L., Bidens cernua L., Corylus avellana L., Echinacea purpurea L. (Moench), Menta piperita L., Trifolium pratense L., Syzygium aromaticum L., and Angelica sinensis (Oliv.) Diels were used. Three species of the genus Candida, namely C. albicans, C. glabrata, and C. tropicalis, isolated from polluted estuarine water, were used in this study. The chemical composition of all EOs was evaluated by gas chromatography coupled with mass spectrometry (GC–MS) and gas chromatography with flame ionization detector (GC–FID) analysis. Firstly, the susceptibility of Candida spp. strains to EXs and EOs was screened by the agar disc diffusion method. Then the minimum inhibitory concentration (MIC) of the tested EXs and EOs was determined in a concentration range from 256 to 0.125 µL/mL. Following that, the effective EXs and EOs were divided into two groups (strong effect and weak effect) and tested in combination (strong + weak) at various concentrations depending on the tested strain to determine their potential synergistic effect using the chessboard pattern. The most sensitive strain to treatments with EXs and EOs tested alone and in mixtures was C. albicans. The most potent combinations with other EXs/EOs were observed with Syzygium aromaticum L., for both plant extract and essential oil, respectively. In this study the EOs and EXs tested in combination were much more effective than when acting on the growth of Candida spp. separately. So, the synergistic effect of the combinations of the EXs and EOs can lead to a reduction in the doses of commercial antifungals and can provide an effective way to reduce the infections caused by yeasts of the genus Candida.
{"title":"THE IN VITRO ANTIFUNGAL AND SYNERGISTIC ACTIVITY OF SELECTED PLANT EXTRACTS AND ESSENTIAL OILS AGAINST CANDIDA SPP.","authors":"Miroslava Hlebová, Dominika Veseleniyova, Zuzana Šrámková, Veronika Vojcikova, A. Kováčik, J. Čuboň, Ivana Charousová, Lenka Hutárová, Lukáš Hleba","doi":"10.55251/jmbfs.10167","DOIUrl":"https://doi.org/10.55251/jmbfs.10167","url":null,"abstract":"This study aimed to evaluate the antifungal activity and the potential synergistic activity of ten plant extracts (EXs) and ten essential oils (EOs) obtained from the same plant species, namely Agrimonia eupatoria L., Arctium lappa L., Arnica montana L., Bidens cernua L., Corylus avellana L., Echinacea purpurea L. (Moench), Menta piperita L., Trifolium pratense L., Syzygium aromaticum L., and Angelica sinensis (Oliv.) Diels were used. Three species of the genus Candida, namely C. albicans, C. glabrata, and C. tropicalis, isolated from polluted estuarine water, were used in this study. The chemical composition of all EOs was evaluated by gas chromatography coupled with mass spectrometry (GC–MS) and gas chromatography with flame ionization detector (GC–FID) analysis. Firstly, the susceptibility of Candida spp. strains to EXs and EOs was screened by the agar disc diffusion method. Then the minimum inhibitory concentration (MIC) of the tested EXs and EOs was determined in a concentration range from 256 to 0.125 µL/mL. Following that, the effective EXs and EOs were divided into two groups (strong effect and weak effect) and tested in combination (strong + weak) at various concentrations depending on the tested strain to determine their potential synergistic effect using the chessboard pattern. The most sensitive strain to treatments with EXs and EOs tested alone and in mixtures was C. albicans. The most potent combinations with other EXs/EOs were observed with Syzygium aromaticum L., for both plant extract and essential oil, respectively. In this study the EOs and EXs tested in combination were much more effective than when acting on the growth of Candida spp. separately. So, the synergistic effect of the combinations of the EXs and EOs can lead to a reduction in the doses of commercial antifungals and can provide an effective way to reduce the infections caused by yeasts of the genus Candida.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47816060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Roshdy, M. Abdelhameed, Helmy A. Rashed, Mona S. Abdellateif, Azza Salamony, Sahar S. Abd El-Rahman, S. Shaarawy
Background: The SARS-COV-2 is a worldwide pandemic problem. We developed a herbal extract with potent in-vitro virucidal, anti-inflammatory and immunomodulatory effects called EGIVIR. Our aim is to assess the bioavailability and cytotoxicity of EGYVIR on different organs and biological systems in Sprague Dawley rats as a model of experimental animals. Methods: 128 rats were divided into 16 groups (8 rats each), where Egyvir was assessed in oral doses of 20, 30, and 40 mg/kg body weight, and by inhalation in 0.2, 0.3, and 0.4 mg/kg body weight, four times/day, compared to the control groups. Results: The Egyvir had no significant effect on the blood pressure, pulse, motor activity, histological, hematological, and coagulation profiles. Also, the blood levels of triglycerides, cholesterol, blood glucose, lactate dehydrogenase (LDH), and creatine phosphor kinase (CPK) were not significantly affected. Egyvir had no harmful effect on the kidney and liver functions, blood electrolytes levels and urinary levels of sodium, potassium, and chloride. There was no significant effect on the serum levels of interleukin-1β (IL-1β), IL-2, IL-4, IL-6, IL-10, interferon- γ (IFN-γ), and tumor necrosis factor- α (TNF-α). Additionally, there was no significant change in the levels of Superoxide dismutase (SOD), catalase, reduced glutathione (GSH), and malonaldehyde (MDA) in comparison to the control groups (P<0.05). Conclusion: Egyvir is considered a safe antiviral natural drug. It could be used for the treatment of SARS-COV-2 without any adverse effects when used with the recommended doses. However, these data are a preliminary step for validation in a clinical setting.
{"title":"ASSESSMENT OF THE IMPACT OF THE EGYVIR ON RATS EXPERIMENTAL ANIMALS; A PRECLINICAL STUDY FOR SARS-COV-2 TREATMENT","authors":"W. Roshdy, M. Abdelhameed, Helmy A. Rashed, Mona S. Abdellateif, Azza Salamony, Sahar S. Abd El-Rahman, S. Shaarawy","doi":"10.55251/jmbfs.9658","DOIUrl":"https://doi.org/10.55251/jmbfs.9658","url":null,"abstract":"Background: The SARS-COV-2 is a worldwide pandemic problem. We developed a herbal extract with potent in-vitro virucidal, anti-inflammatory and immunomodulatory effects called EGIVIR. Our aim is to assess the bioavailability and cytotoxicity of EGYVIR on different organs and biological systems in Sprague Dawley rats as a model of experimental animals.\u0000Methods: 128 rats were divided into 16 groups (8 rats each), where Egyvir was assessed in oral doses of 20, 30, and 40 mg/kg body weight, and by inhalation in 0.2, 0.3, and 0.4 mg/kg body weight, four times/day, compared to the control groups. \u0000Results: The Egyvir had no significant effect on the blood pressure, pulse, motor activity, histological, hematological, and coagulation profiles. Also, the blood levels of triglycerides, cholesterol, blood glucose, lactate dehydrogenase (LDH), and creatine phosphor kinase (CPK) were not significantly affected. Egyvir had no harmful effect on the kidney and liver functions, blood electrolytes levels and urinary levels of sodium, potassium, and chloride. There was no significant effect on the serum levels of interleukin-1β (IL-1β), IL-2, IL-4, IL-6, IL-10, interferon- γ (IFN-γ), and tumor necrosis factor- α (TNF-α). Additionally, there was no significant change in the levels of Superoxide dismutase (SOD), catalase, reduced glutathione (GSH), and malonaldehyde (MDA) in comparison to the control groups (P<0.05).\u0000Conclusion: Egyvir is considered a safe antiviral natural drug. It could be used for the treatment of SARS-COV-2 without any adverse effects when used with the recommended doses. However, these data are a preliminary step for validation in a clinical setting.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43079798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monika Ňorbová, A. Vollmannová, Eduard Pintér, Marek Šnirc, H. Franková, Silvia Fedorková, Natália Čeryová
This study aims to determine the vitamin C content and antioxidant activity in different types of fruit: cornelian cherry (Cornus mas L.), service tree (Sorbus domestica), medlar (Mespilus germanica L.), pawpaw (Asimina triloba L.), persimmon (Diospyros kaki L.). The aim of the work was to compare these used methods. The fruit’s vitamin C content was determined by HPLC system Waters Separations Module 2695 with UV detector 2996. The content of vitamin C reached values from 72.238 µg. g-1 (Diospyros kaki L.) to 1114.61 µg.g-1 (Sorbus domestica). The antioxidant activity (DPPH) was determined spectrophotometrically. DPPH radical scavenging activity values were 2.28 (Mespilus germanica L.) – 12.34 μmol TE.g-1 DW (Cornus mas L.). Spearman's test showed a positive relationship between both monitored parameters. The content of vitamin C in the studied species showed a positive relationship with antioxidant activity. Due to the high content of vitamin C and antioxidant activity, future analyses should focus on the determination of other bioactive substances in these fruits.
本研究旨在测定不同类型果实的维生素C含量和抗氧化活性：山茱萸（Cornus mas L.）、服务树（Sorbus domestica）、枸杞（Mespilus germanica L.）、木瓜（Asimina triloba L.）、柿子（Diospyros kaki L.）。通过HPLC系统Waters Separations Module 2695和UV检测器2996测定水果的维生素C含量。维生素C的含量达到72.238µg。g-1（Diospyros kaki L.）至1114.61µg.g-1（Sorbus domestica）。采用分光光度法测定其抗氧化活性（DPPH）。DPPH自由基清除活性值为2.28（德国麦穗）-12.34μmol TE.g-1 DW（美国玉米）。Spearman试验显示两个监测参数之间呈正相关。研究物种的维生素C含量与抗氧化活性呈正相关。由于维生素C和抗氧化活性含量高，未来的分析应侧重于测定这些水果中的其他生物活性物质。
{"title":"THE CONTENT OF VITAMIN C AND ANTIOXIDANT ACTIVITY IN LESS-KNOWN TYPES OF FRUIT","authors":"Monika Ňorbová, A. Vollmannová, Eduard Pintér, Marek Šnirc, H. Franková, Silvia Fedorková, Natália Čeryová","doi":"10.55251/jmbfs.9937","DOIUrl":"https://doi.org/10.55251/jmbfs.9937","url":null,"abstract":"This study aims to determine the vitamin C content and antioxidant activity in different types of fruit: cornelian cherry (Cornus mas L.), service tree (Sorbus domestica), medlar (Mespilus germanica L.), pawpaw (Asimina triloba L.), persimmon (Diospyros kaki L.). The aim of the work was to compare these used methods. The fruit’s vitamin C content was determined by HPLC system Waters Separations Module 2695 with UV detector 2996. The content of vitamin C reached values from 72.238 µg. g-1 (Diospyros kaki L.) to 1114.61 µg.g-1 (Sorbus domestica). The antioxidant activity (DPPH) was determined spectrophotometrically. DPPH radical scavenging activity values were 2.28 (Mespilus germanica L.) – 12.34 μmol TE.g-1 DW (Cornus mas L.). Spearman's test showed a positive relationship between both monitored parameters. The content of vitamin C in the studied species showed a positive relationship with antioxidant activity. Due to the high content of vitamin C and antioxidant activity, future analyses should focus on the determination of other bioactive substances in these fruits.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44724157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Herein we report a sunlight-driven, instant, one-step, green route for the synthesis of silver nanoparticles (AgNPs) using aqueous fruit extract of Terminalia paniculata Roth and in vitro assessment of its antioxidant and anti-inflammatory activities. 5 mL of aqueous fruit extract of T. paniculata was added to 95 mL of 1 mM AgNO3 and exposed to direct sunlight. The T. paniculata fruit extract mediated AgNPs (TpF-AgNPs) were characterized for their morphological, physical, and chemical properties using UV-Vis Spectroscopy, Fourier-Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and High-Resolution Transmission Electron Microscopy (HR-TEM) with Selected Area Electron Diffraction (SAED). The antioxidant activity of TpF-AgNPs was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay and the anti-inflammatory activity by Bovine Serum Albumin (BSA) anti-denaturation assay at different doses. The formation of TpF-AgNPs was observed as a colour change from pale-yellow to reddish-brown within 3 mins under direct sunlight. UV-Vis spectra revealed an absorption peak at 411 nm, which is the characteristic for silver nanoparticles. FTIR spectra revealed the possible functional groups of phytochemicals such as -OH and -NH involved in the reduction and capping of TpF-AgNPs. HR-TEM analysis confirmed the spherical shape of nanoparticles with sizes ranging from 5-50 nm with an average particle size of 26 nm. AFM shows surface topography of TpF-AgNPs. The crystalline nature with Face Centered Cubic structure was confirmed through XRD and SAED. The negative value of zeta potential (-53.9 mV) indicated the excellent stability of nanoparticles. The TpF-AgNPs exhibited maximum % scavenging of 71.72 ± 0.51 at 100 µg/mL for antioxidant activity and maximum % inhibition of 85.88 ± 1.09 at 500 µg/mL for anti-inflammatory activity. The sunlight-driven green synthesis of TpF-AgNPs proved to be an instant method in the formation of small-sized nanoparticles exhibiting good antioxidant and anti-inflammatory activity.
{"title":"SUNLIGHT-DRIVEN INSTANT SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS FRUIT EXTRACT OF TERMINALIA PANICULATA ROTH AND IN VITRO ASSESSMENT OF ITS ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES","authors":"Nirupa Vernekar, T. Taranath","doi":"10.55251/jmbfs.9236","DOIUrl":"https://doi.org/10.55251/jmbfs.9236","url":null,"abstract":"Herein we report a sunlight-driven, instant, one-step, green route for the synthesis of silver nanoparticles (AgNPs) using aqueous fruit extract of Terminalia paniculata Roth and in vitro assessment of its antioxidant and anti-inflammatory activities. 5 mL of aqueous fruit extract of T. paniculata was added to 95 mL of 1 mM AgNO3 and exposed to direct sunlight. The T. paniculata fruit extract mediated AgNPs (TpF-AgNPs) were characterized for their morphological, physical, and chemical properties using UV-Vis Spectroscopy, Fourier-Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and High-Resolution Transmission Electron Microscopy (HR-TEM) with Selected Area Electron Diffraction (SAED). The antioxidant activity of TpF-AgNPs was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay and the anti-inflammatory activity by Bovine Serum Albumin (BSA) anti-denaturation assay at different doses. The formation of TpF-AgNPs was observed as a colour change from pale-yellow to reddish-brown within 3 mins under direct sunlight. UV-Vis spectra revealed an absorption peak at 411 nm, which is the characteristic for silver nanoparticles. FTIR spectra revealed the possible functional groups of phytochemicals such as -OH and -NH involved in the reduction and capping of TpF-AgNPs. HR-TEM analysis confirmed the spherical shape of nanoparticles with sizes ranging from 5-50 nm with an average particle size of 26 nm. AFM shows surface topography of TpF-AgNPs. The crystalline nature with Face Centered Cubic structure was confirmed through XRD and SAED. The negative value of zeta potential (-53.9 mV) indicated the excellent stability of nanoparticles. The TpF-AgNPs exhibited maximum % scavenging of 71.72 ± 0.51 at 100 µg/mL for antioxidant activity and maximum % inhibition of 85.88 ± 1.09 at 500 µg/mL for anti-inflammatory activity. The sunlight-driven green synthesis of TpF-AgNPs proved to be an instant method in the formation of small-sized nanoparticles exhibiting good antioxidant and anti-inflammatory activity.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45015437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Campi, Claudia Mancuello, Francisco Ferreira, Y. Maubet, Enzo Cristaldo, Elvio Gayoso, G. Robledo
As a continuation of the study of the biologically active compounds of native neotropical Ganoderma species in Paraguay, the content of total phenolic compounds, total antioxidants and percentage of antioxidant activity of the fractions extracted with ethanol, hexane, diethyl ether, ethyl acetate and water of mycelium in liquid medium, wild and cultivated fruiting bodies of G. tuberculosum, were analyzed. Biologically active compounds were highest in the diethyl ether fractions of wild fruiting bodies, 126.49 ± 4.19 mg. (GAE) g-1 (milligrams of Gallic acid equivalent per grams of crude extract), antioxidant compounds content of 130.94 ± 5.302 mg .g-1 AAE (milligrams of Ascorbic acid equivalent per grams of crude extract) and activity percentage of 70.93% compared to 67.39 ± 1.5 mg. (GAE) g-1, 48.37 ± 0.73.g-1 AAE and 45.63% antioxidant activity. According to the values obtained in the present study, the fruiting body of Ganoderma tuberculosum is a promising source of antioxidant compounds for the food industry.
{"title":"DOES THE SOURCE MATTER? PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY FROM MYCELIUM IN LIQUID MEDIUM, WILD AND CULTIVATED FRUITING BODIES OF THE NEOTROPICAL SPECIES GANODERMA TUBERCULOSUM","authors":"M. Campi, Claudia Mancuello, Francisco Ferreira, Y. Maubet, Enzo Cristaldo, Elvio Gayoso, G. Robledo","doi":"10.55251/jmbfs.6148","DOIUrl":"https://doi.org/10.55251/jmbfs.6148","url":null,"abstract":"As a continuation of the study of the biologically active compounds of native neotropical Ganoderma species in Paraguay, the content of total phenolic compounds, total antioxidants and percentage of antioxidant activity of the fractions extracted with ethanol, hexane, diethyl ether, ethyl acetate and water of mycelium in liquid medium, wild and cultivated fruiting bodies of G. tuberculosum, were analyzed. Biologically active compounds were highest in the diethyl ether fractions of wild fruiting bodies, 126.49 ± 4.19 mg. (GAE) g-1 (milligrams of Gallic acid equivalent per grams of crude extract), antioxidant compounds content of 130.94 ± 5.302 mg .g-1 AAE (milligrams of Ascorbic acid equivalent per grams of crude extract) and activity percentage of 70.93% compared to 67.39 ± 1.5 mg. (GAE) g-1, 48.37 ± 0.73.g-1 AAE and 45.63% antioxidant activity. According to the values obtained in the present study, the fruiting body of Ganoderma tuberculosum is a promising source of antioxidant compounds for the food industry.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48104751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maryam Armand, M. Faezi Ghasemi, M. Fazeli, M. Mirpour
Probiotics such as Lactobacilli are important in improving normal intestinal flora and hindering the growth of harmful bacteria in the digestive system. Given the above reasons, the industrial production of probiotics and the use of high-yield strains is of great importance. The present study compares the biomass production by Lactobacillus rhamnosus GG ATCC 53103 in batch and fed-batch cultures conditions at a pilot plant scale. An optimized medium containing the following compounds (g/L): glucose 112.50, sugar beet molasses 56.25, casein 18.75, yeast extract 18.75, K2HPO4 13.13, Tween 80 1.88, MgSO4.7H2O 0.3750, MnSO4. 4H2O 0.0750, CaCl2. 2H2O 0.1875 and Simethicone 1.25 was used for biomass production. During the fermentation process, culture conditions such as pH, temperature, and oxygen concentration were monitored using process analytical technology (PAT). Based on the obtained results, the maximum biomass production in the batch condition in the first 20 hours of culture in the optimized medium was about 68.14 g/L. After three stages of fed-batch culture, the biomass production by L. rhamnosus GG ATCC 53103 reached 93.5 g/L at 37°C with agitation and aeration rates of 100 rev/min and 300 VV-1min-1, respectively. Therefore, biomass production increased about 2.67-fold more than the basal medium.
{"title":"PILOT-PLANT SCALE BIOMASS PRODUCTION BY LACTOBACILLUS RHAMNOSUS GG ATCC 53103: A COMPARISON BETWEEN BATCH AND FED-BATCH FERMENTATION","authors":"Maryam Armand, M. Faezi Ghasemi, M. Fazeli, M. Mirpour","doi":"10.55251/jmbfs.9718","DOIUrl":"https://doi.org/10.55251/jmbfs.9718","url":null,"abstract":"Probiotics such as Lactobacilli are important in improving normal intestinal flora and hindering the growth of harmful bacteria in the digestive system. Given the above reasons, the industrial production of probiotics and the use of high-yield strains is of great importance. The present study compares the biomass production by Lactobacillus rhamnosus GG ATCC 53103 in batch and fed-batch cultures conditions at a pilot plant scale. An optimized medium containing the following compounds (g/L): glucose 112.50, sugar beet molasses 56.25, casein 18.75, yeast extract 18.75, K2HPO4 13.13, Tween 80 1.88, MgSO4.7H2O 0.3750, MnSO4. 4H2O 0.0750, CaCl2. 2H2O 0.1875 and Simethicone 1.25 was used for biomass production. During the fermentation process, culture conditions such as pH, temperature, and oxygen concentration were monitored using process analytical technology (PAT). Based on the obtained results, the maximum biomass production in the batch condition in the first 20 hours of culture in the optimized medium was about 68.14 g/L. After three stages of fed-batch culture, the biomass production by L. rhamnosus GG ATCC 53103 reached 93.5 g/L at 37°C with agitation and aeration rates of 100 rev/min and 300 VV-1min-1, respectively. Therefore, biomass production increased about 2.67-fold more than the basal medium.","PeriodicalId":16348,"journal":{"name":"Journal of microbiology, biotechnology and food sciences","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45088655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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